| urea-hydrolyzing activity of a t-strain mycoplasma: ureaplasma urealyticum. | the urea-hydrolyzing activity of a t-strain mycoplasma was studied in experiments using whole cells and cell-free enzyme preparations by measuring the release of 14co2 from 14curea. under the conditions used, the urea concentration optimum is approximately 5.6 x 10(-3) m urea. the activity is soluble and not membrane bound. it is stable at -70 c for several weeks but is more labile at higher temperatures. the ph optimum is between 5.0 and 6.0. the effect of several inhibitors on the activity was ... | 1976 | 1381 |
| immunofluorescence, bacteriological and patho-morphological studies of the organs of respiration of children who died of acute pneumonia. | | 1975 | 1919 |
| in vitro susceptibility studies with josamycin and erythromycin. | the in vitro activity of josamycin and erythromycin against five bacterial species was compared. in general, erythromycin was slightly more active by weight than josamycin, although both agents had a similar range of activity. | 1976 | 4012 |
| associated seroconversions to respiratory viruses in volunteers with experimental influenza infection. | serological examinations of 573 volunteers with mild experimental influenza infection and 86 volunteers of a control group hospitalized in a special clinic revealed a significant rise in the titre of antibodies (seroconversion) not only to influenza a or b viruses used for the experimental infection but in 23.3 to 29.8% of cases also to other respiratory viruses. based on a number of arguments, associated seroconversions are interpreted as due to mixed or sequential infections of different aetio ... | 1976 | 5868 |
| cultivation of mycoplasmas in a modified tissue culture medium. | a new medium, which contained a chemically defined tissue culture base ("medium 199"), was developed for the cultivation of mycoplasmas. when supplemented with albumin, glucose, serum, and yeast extract, the new medium adequately supported the growth of mycoplasma and acholeplasma species. | 1976 | 7199 |
| reduced nicotinamide adenine dinucleotide oxidase activity in membranes and cytoplasm of acholeplasma laidlawii and mycoplasma mycoides subsp. capri. | the properties of the membrane-bound reduced nicotinamide adenine dinucleotide (nadh) oxidase of acholeplasma laidlawii were compared with those of the corresponding cytoplasmic activity of mycoplasma mycoides subsp. capri. the striking differences in ph optima, susceptibility to inhibitors and detergents, and heat inactivation between the nadh oxidase activity, with oxygen as an electron acceptor, and the nadh oxidoreductase activity, with dichlorophenol indophenol (dcpip) as an alternate elect ... | 1976 | 11208 |
| recent advances in equine reproduction. | mares rarely ovulate in winter; ovulation is induced by increase in daylight length. ova accumulate in the oviducts of unserved mares. during pregnancy, corpora lutea accumulate; all regress together at mid pregnancy. plasma progesterone levels rise and oestrogen levels fall towards the end of pregnancy. methods are available for early termination of pregnancy and for induction of parturition. pregnancy can be diagnosed efficiently by rectal examination, and by immunological assay of pregnant ma ... | 1977 | 13992 |
| motility of mycoplasma pneumoniae. | cell of mycoplasma pneumoniae fh gliding on a glass surface in liquid medium were examined by microscopic observation and quantitatively by microcinematography (30 frames per min). comparisons were made only within the individual experiments. the cells moved in an irregular pattern with numerous narrow bends and circles. they never changed their leading end. the average speed (without pauses) was relatively constant between o.2 and 0.5 mum/s. the maximum speed was about 1.5 to 2.0 mum/s. the mov ... | 1977 | 14925 |
| synthesis and biological activity of some vinyl-substituted 2-nitroimidazoles. | in previous studies 1-methyl-2-nitro-1h-imidazole-5-carboxaldehyde and 1-methyl-2-nitro-5-vinyl-1h-imidazole were found to posses interesting antimicrobial activities. we have now prepared some 2-nitro-1h-imidazoles in which the 5-vinyl chain bears selected functional groups (cho, coch3, no2) as well as nitrogen-condensation derivatives of the carbonyl functions. furthermore, 5-methyl-2-nitro-1-vinyl-1h-imidazole has been synthesized. all the compounds, and some intermediates, have been assayed ... | 1977 | 16132 |
| clinical manifestations due to or associated with mycoplasma pneumoniae infection in adults. | | 1977 | 17904 |
| etiology of acute pneumonia in young children. | | 1977 | 18702 |
| hepatotoxicity of erythromycin estolate during pregnancy. | women in the second half of pregnancy, who were infected with genital mycoplasmas and who gave written informed consent, were randomly assigned to receive capsules of identical appearance containing erythromycin estolate, clindamycin hydrochloride, or a placebo for 6 weeks. levels of serum glutamic oxalacetic transaminase (sgot) were determined before and during treatment by a fluorometric method. all pretreatment levels of sgot were normal (<41 units). participants who received erythromycin est ... | 1977 | 21610 |
| induction of interferon in mice and cell cultures by mycoplasma pneumoniae. | inoculation of mice and l and human embryonic lung (hel) cell cultures with mycoplasma pneumoniae failed to induce the production of interferon. m. pneumoniae multiplied in these cell cultures without a marked cytopathic effect. m. pneumoniae induced interferon in human peripheral blood leukocytes with maximum titres of 32 units/ml at 48 hours after infection. | 1977 | 23667 |
| exacerbations of chronic bronchitis: exogenous or endogenous infection? | six male patients with chronic bronchitis, who were known previously to have excreted streptococcus pneumoniae and/or haemophilus influenzae, both at the times of exacerbations and during remission, were studied for 43 to 52 months. sputum was examined fortnightly and at the time of exacerbations. strains of strep. pneumoniae were serotyped and those of haemophilus species were typed by antibiograms along with other supporting methods. sera collected before or at the time of an exacerbation and ... | 1978 | 25664 |
| effect of osmotic potential, ph, and temperature on the growth of a helical, motile mycoplasma causing corn stunt disease. | growth characteristics of corn stunt spiroplasma, a helical, motile mycoplasma, were studied over a range of osmolality, ph, and temperature in a simple medium containing 20% (v/v) agamma horse serum, 1.5% (w/v) pplo broth, and various concentrations of sucrose. the spiroplasma was able to grow in a wide spectrum of osmolalities from 360 to 1120 mosm. optimal growth was observed in media that contained 0.25-0.35 m sucrose. the organism became longer and thinner in media adjusted to 0.65 m sucros ... | 1978 | 25704 |
| caseinolytic activity of human mycoplasmas. | | 1978 | 26478 |
| initiation and characterization of a diploid cell line from larval tissues of aedes dorsalis (meigen). | mosquito cell cultures were initiated from the minced tissues of newly hatched aedes dorsalis (meigen) larvae. continuous cell division occurred only after an adaptive period of approximately 6 months. optimal growth of the cells required a relatively low ph of 6.5. karyological studies showed that the cells have remained diploid (2n = 6) for 60 serial passages and that the cultures are free of contaminating cells. the cultures also were shown to be free of bacteria (including mycoplasma), fungi ... | 1978 | 27445 |
| immunochemical studies on a mycoplasma pneumoniae polysaccharide fraction: cross-reactions with type 23 and 32 antipneumococcal rabbit sera. | lipid-free polysaccharide fraction 2 extracted from mycoplasma pneumoniae strain fh by prescott et al. (j. bacteriol. 91:2117-2115, 1966) was examined for its ability to cross-precipitate antibody from type-specific rabbit antipneumococcal sera types 1 to 34 inclusive. cross-precipitation in type-specific pneumococcal anti-type 23 and anti-type 32 sera was examined in detail and could be attributed to a rhamnose-galactose-rich component of crude m. pneumoniae polysaccharide fraction 2 recovered ... | 1978 | 27459 |
| macrolide antibiotics m-4365 produced by micromonospora. iii. in vitro antimicrobial activity of antibiotic m-4365g2 (de-epoxy rosamicin). | antibiotic m-4365g2 (de-epoxy rosamicin) produced by micromonospora capillata mcrl 0940 is a new basic 16-membered macrolide antibiotic with activity equal to or superior to erythromycin and josamycin against gram-positive bacteria. of interest are the high degree of activity against gram-negative bacilli and mycoplasmas, and striking inhibitory effects against indole-producing proteus spp. bactericidal activity of m-4365g2 is also to be noticed. | 1978 | 27495 |
| morphological characteristics of acute respiratory tract diseases of mixed etiology in children. | the analysis of 30 etiologically deciphered (by the direct coons method) autopsy observations of acute respiratory diseases in children under 8 is presented. monoinfections were established only in 6 cases. morphological examinations of respiratory organs in 24 cases of mixed acute respiratory diseases (associations of virus-bacteria and virus-mycoplasma pneumoniae-diplostreptococcus) revealed marked circulatory disorders and desquamative, dystrophic, and inflammatory processes in segmental bron ... | 1978 | 28114 |
| the value of immunoelectroosmophoresis (ieop) for etiological diagnosis of acute respiratory tract infections due to pneumococci and mycoplasma pneumoniae. | immunoelectroosmophoresis (ieop) has been used to detect pneumococcal and mycoplasma pneumoniae antigen in 324 sputum samples from 224 patients. pneumococcal antigen was found in 30/37 samples from which pneumococci had earlier been isolated and in 72/243 specimens where they had not been found. of these 72 samples 69 were from patients treated with antibiotics. mycoplasma antigen was found in 9/57 sputum samples from which mycoplasma had been isolated and in 2/32 other samples from patients wit ... | 1978 | 30990 |
| serological diagnosis of pneumococcal disease with enzyme-linked immunosorbent assay (elisa). | antibody response to pneumococcal type-specific polysaccharide was measured with enzyme-linked immunosorbent assay (elisa). the test was shown to be up to 500 times more sensitive than indirect hemagglutination using chromic chloride-treated red blood cells. in 16/17 patients with pneumococcal pneumonia a significant antibody increase was seen as measured with elisa. only 6/23 patients with pneumonia caused by mycoplasma pneumoniae gave a pneumococcal antibody rise and in those cases the increas ... | 1978 | 30991 |
| eperythrozoon ovis. iii. the effect of infection on blood ph, concentrations of pyruvate, lactate and glucose in blood, haematology, and on concurrent and superimposed trypanosoma vivax infection in sheep. | infections of sheep with eperythrozoon ovis has been shown to increase pyruvate and lactate concentrations in blood and to reduce blood ph over a period of 10-14 days post-inoculation. there was no effect on the concentration of blood glucose. however, if care is not taken to inhibit glycolysis, the concentration of blood glucose in samples of blood taken for analysis falls markedly. rapid destruction of erythrocytes started about 10 days post-inoculation and was progressive. superimposition of ... | 1978 | 33476 |
| the microbiology of serous and mucoid otitis media. | one hundred forty-four serous and mucoid effusions were cultured for aerobic bacteria, mycoplasma pneumoniae, and virus. thirty percent of all effusions yielded an unequivocally positive culture for aerobic bacteria. although serous effusions were culture positive as often as mucoid effusions, haemophilus influenzae was isolated predominantly from serous effusions and staphylococcus epidermidis predominantly from mucoid samples. only one of 73 effusions yielded a viral isolate (herpesvirus homin ... | 1979 | 36591 |
| adherence of erythrocytes to mycoplasma pneumoniae. | the human pathogen mycoplasma pneumoniae adheres to a variety of cells, including erythrocytes. a hemadsorption technique was developed to quantitate adherence by photometric measurement of lysates of erythrocytes that attached to sheets of m. pneumoniae grown in cups of linbro plates. attachment of sheep erythrocytes (se) increased with higher ionic strength, was unaffected by minor ph variations (6 to 9), and was blocked by anti-m. pneumoniae antiserum, but was not inhibited by a variety of su ... | 1979 | 39034 |
| adherence of mycoplasma pneumoniae to glass surfaces. | attachment of m. pneumoniae to glass was quantitated in an experimental system enabling the settling down of [3h]palmitic acid-labeled cells onto glass cover slips. attachment of mycoplasmas suspended in buffer increased with temperature, decreased with higher ionic strength, and showed a maximum at about ph 5.5. the findings suggest a participation of ionic bonds in the attachment process. trypsin did not detach glass-bound mycoplasmas, and treatment of the cells with glutaraldehyde did not red ... | 1979 | 40883 |
| pathogenicity of mycoplasmas for man, animals, plants and insects. xii international congress of microbiology, münchen, september 3-8, 1978. | | 1979 | 44606 |
| pathogenesis of mycoplasma pneumoniae disease. | | 1979 | 44607 |
| autoimmune reactions associated with mycoplasma pneumoniae infection. | patients with mycoplasma pneumoniae infection often develop various autoantibodies including cold agglutinins, cold antibodies reacting with lymphocytes, antibodies against various tissue antigens, for instance brain and lung and smooth muscle antibodies. various mechanisms that may be responsible for the induction of these autoimmune responses and the possible pathogenicity of these autoantibodies are discussed. | 1979 | 44608 |
| pathogenicity of ureaplasmas for animals and man. | since their original isolation from the genital tract of man, ureaplasmas, previously termed t-strain mycoplasmas, have been isolated from a variety of animal species. under experimental conditions they have been shown to cause mastitis in cattle, goats and mice, and observations made on naturally-occurring bovine pneumonia, as well as the results of experimental inoculation, suggest that ureaplasmas are responsible for a portion of bovine cuffing pneumonia. ureaplasmas have been isolated from t ... | 1979 | 44609 |
| pathogenicity of the subspecies mycoides of mycoplasma mycoides for cattle, sheep and goats. | recent work has shown that strains classified as m. mycoides subsp. mycoides may be separated into 2 types according to their growth rate and their behaviour in certain biochemical tests. the large colony (lc) types, most of which are from goats, are pathogenic for sheep and goats but apparently not for cattle. the small colony (sc) types include the classical contagious bovine pleuropneumonia (cbpp) strains from cattle and four strains from goats. these sc types are potentially pathogenic for c ... | 1979 | 44610 |
| the pathogenicity of avian mycoplasmas. | based on literature data and own experiences the author gives an outlook about pathogenicity of avian mycoplasmas. in chickens and turkeys m. gallisepticum and m. synoviae (in addition to it m. meleagridis exclusively in turkeys) are the most important mycoplasmas producing respiratory disease, inflamation of synovial membranes and other lesions. their pathogenic effect is very much influenced by dose of agent, route of entry of microorganism, age of birds, virulence and tropism of organism as w ... | 1979 | 44612 |
| [method of obtaining biological masses of mycoplasma]. | | 1975 | 45966 |
| letter: nitroblue-tetrazolium test. | | 1975 | 45995 |
| a simple cytochemical technique for demonstration of dna in cells infected with mycoplasmas and viruses. | | 1975 | 46112 |
| morphologic observations of mycoplasmas and neisseria gonorrhoeae in associated growth patterns. | mycoplasma hominis and t-colony mycoplasmas were observed growing among or developing within gonococcal colonies on primary isolation plates streaked for detection of n. gonorrhoeae in urogenital specimens. the frequency of such association of mycoplasmas with gonococci was as high as 84% among patients screened for gonorrhea in several social hygiene clinics of the city of new york, department of health, bureau of venereal disease control. the individual morphology of both mycoplasmal and gonoc ... | 1975 | 46128 |
| antigenic and enzymatic changes in infected and transformed human diploid cells. | human diploid fibroblasts, wi-38, were infected with various agents and the levels of lysosomal enzymes determined by immunochemical quantitation. esterase levels were raised by mumps virus and toxoplasma gondii infection. the concentration of beta-d-glucuronidase was reduced by these same agents. beta-d-n-acetyl glucosaminidase was greatly increased in cultures infected with t. gondii and decreased by mycoplasma infection. two cultures transformed by sv40 showed reduced levels of esterase compa ... | 1975 | 46837 |
| histamine release from human leukocytes when stimulated by mycoplasma salivarium. | incubated mixtures of mycoplasma salivarium and nonsensitized mixed leukocytes released histamine. spectrophotofluorometric detection of histamine occurred at physiological temperature and ph independent of complement and specific antiserum. | 1975 | 46845 |
| a virological and epidemiological study of patients with acute hearing loss. | virological and epidemiological data were examined in thirty-nine patients with acute hearing loss. the hearing loss was unilateral in thirty-one patients and bilateral in eight. there was a history of upper-respiratory-tract infection before onset of deafness in ten of the patients. cases were more common in the autumn, with nineteen of the cases occurring in the 3 months from september to november. about half of the patients were 20-40 years old and half 46-73 years old; only one patient w ... | 1975 | 46953 |
| investigation into the relationship of m ulcerans to m. buruli and other mycobacteria. | the main characteristics of mycoplasma ulcerans are its remarkably distinct drug sensitivity pattern and its rather poor enzymatic activity. m. microti is clearly distinguishable from m. bovis and m. tuberculosis. some of the m. buruli strains appear to be identical with m. ulcerans, whereas others show similarity either to m. microti or to m. tuberculosis. it seems that m. buruli is not a legitimate species. | 1975 | 47730 |
| ultrastructure of lipopolysaccharide isolated from thermoplasma acidophilum. | the fine structure of lipopolysaccharide isolated from thermoplasma acidophilum was examined by electron microscopy. negative staining of the lipopolysaccharide revealed long, ribbon-like structures with some branching. the average width of the lipopolysaccharide ribbons was 5 nm. treatment of the lipopolysaccharide with 0.5% sodium dodecyl sulfate resulted in the dissociation of the ribbon-like structures to spherical- and vesicular-shaped particles and some short, rodlike structures. results s ... | 1975 | 46226 |
| turkey syndrome 65, oedema syndrome and mycoplasma meleagridis. | comparisons of liver histology and serum protein characteristics from cases of oedema syndrome and from poults with mycoplasma meleagridis induced ascites revealed that the two conditions, though presenting grossly similar post mortem appearances, are different entities. serum albumin concentrations, but not total serum protein levels, were markedly reduced in poults with m meleagridis induced ascites and in those with turkey syndrome 65. poults which were infected with m meleagridis but which f ... | 1975 | 48271 |
| t-mycoplasmas: a study of the morphology, ultrastructure and mode of division of some human strains. | the morphology of 10 strains of t-mycoplasma was studied in wet preparations of viable cells by darkfield, phase-contrast and interference microscopy, and in fixed preparations by various techniques of electron microscopy. mycoplasma-like artefacts in the horse-serum component of the medium were eliminated by filtration. all 10 strains were similar. individual cells were spherical, 0-25-1-0 mum in size, with a bounding trilaminar membrane, 10 nm thick and containing 7-5-12-5-nm particles, and a ... | 1975 | 49431 |
| [the differenziation of swine-mycoplasma by acrylamide-gel-electrophoresis (author's transl)]. | the strains of swine mycoplasma (tab. 1) were grown aerobically in whittlestone's medium containing swine serum for 4-5 days at 37 degrees c. after centrifugation and washing they were freeze-dried and extracted with a phenol-acetic acid-water solution (4:2:1) (1 ml/5 mg mycoplasma dru weight) at 4 degrees c over 48 h. after centrifugation 2 volumes of the supernatant were mixed with 1 volume of a 40% sucrose solution in 35% acetic acid. 0,15 or 0,2 ml of which were added to the acrylamide-gel ( ... | 1975 | 49991 |
| the skin-reactive antigens of mycoplasma pneumoniae. | guinea pigs experimentally infected with mycoplasma pneumoniae or immunized with the orgnaism in combination with freund's complete adjuvant developed a delayed hypersensitive skin reaction following on intradermal injection of the m. pneumoniae antigen. the amount of protein necessary to produce the delayed skin reaction was as low as 0.01 mug. when the sonicated whole cells were extracted with aqueous acetone, the delayed skin reactivity was found mostly in the acetone insoluble (lipid-deplete ... | 1975 | 50469 |
| [identification of mycoplasma isolated from the male urogenital tract]. | | 1975 | 50491 |
| proceedings: krebs-henseleit urea cycle in cultured human diploid fibroblasts. | | 1975 | 50817 |
| virus retrieval studies in human neoplasia. | short- and long-term co-cultures of 49 cases of human osteosarcoma cells with bone marrow or peripheral blood cells of patients with different types of leukemia were studied. morphological changes were observed in 7 of 13 long-term co-cultures resembling those induced by rna tumor viruses. the changes were accompanied by appearance of cytoplasmic antigen as shown by fixed immunofluorescence test with sera from patients with osteosarcoma, leukemia, and of some apparently normal blood donors. abso ... | 1975 | 51629 |
| pathogenicity of mycoplasmas for arthropods. | | 1979 | 44614 |
| the pathogenicity of mycoplasmas for plants. | many plant diseases belonging to the yellows group are believed to be caused by wall-free prokaryotes resembling mycoplasmas, which are spread by leafhopper vectors. im most cases the evidence for mycoplasma aetiology rests upon the finding by electron microscopy of mycoplasma-like bodies in phloem tissue of diseased plants, coupled in some cases with symptom remission following treatment of plants with tetracyclines. the only plant-pathogenic mycoplasmas which have so far been cultured are the ... | 1979 | 44613 |
| adherence of mycoplasma gallisepticum to glass. | attachment of washed mycoplasma gallisepticum cells to glass was quantified with organisms in which membrane lipids were labelled with 3h. siliconization of the test tubes decreased attachment, while centrifugation increased it. attachment increased with temperature, decreased with increasing ph and ionic strength of the attachment mixture, but was unaffected by ca2+, mg2+ and edta. this suggests that ionic bonds, but not salt bridges, participate in the attachment process. glycophorin, the majo ... | 1979 | 37284 |
| active k+ transport in mycoplasms mycoides var. capri. relationships between k+ distribution, electrical potential and atpase activity. | the addition of 5 . 10(-5) m or less of dicyclohexylcarbodiimide to mycoplasma mycoides var. capri preferentially influences k+ influx rather than efflux and reduces by 30--40% the activity of the membrane-bound mg2+- atpase. adding valinomycin to metabolizing cells does not markedly affect k+ distribution but induces a rapid and complete loss of intracellular k+ in non-metabolizing cells. uncoupling agents such as dinitrophenol, carbonylcyanide p-trifluoromethoxyphenylhydrazone, dissipate the k ... | 1979 | 36912 |
| effect of preparatory techniques on the gross morphology of mycoplasma hominis. | a method is presented whereby cells of mycoplasma hominis can be prepared with minimal distortion for electron microscopy. after the addition of glutaraldehyde to broth cultures, incubation is continued for 1 h. the cells are then collected by centrifugation, washed in distilled water, and used for negative-contrast preparations. | 1975 | 52651 |
| size and shape of mycoplasma virus mv-lg-l 172 dna. | sedimentation of dna of mycoplasma virus mv-lg-l 172 in neutral and alkaline density gradients showed that the chromosome of this virus is most probably formed by a double-stranded circular dna molecule with a molecular weight of approx. 10(7) daltons. these results were confirmed by direct electron microscopy of the viral dna. gamma-irradiation of the virus caused in its dna single breaks on the average per each 66 ev of absorbed energy. virus survival depended exponentially on the irradiation ... | 1978 | 35941 |
| [value of the antibiogram for the treatment of urogenital ureaplasma infections]. | we have currently established the spectrum of sensitivity of the isolated strains to various antibiotics before prescribing the appropriate treatment. a microtechnic has been used. the most active antibiotics are: minocyclin, pristinamycin, erythromycin, chloramphenicol, oxytetracyclin, kanamycin, tobramycin, streptomycin, in this order. | 1978 | 34136 |
| prostatitis: man's hidden infection. | prostatitis exists when inflammation of prostatic glands and tissues results from infection or allergy. gram-positive and negative bacteria cause most prostatic infections, but infections may also be caused by fungi, mycoplasma, viruses, and other nonbacterial infecting agents. precise diagnostic localization of infection to prostatic glands is accomplished by obtaining divided urinary specimens and prostatic fluid and observing numbers of bacteria (or other infecting agents) present in each spe ... | 1975 | 52931 |
| the practice laboratory: the interpretation of blood smears. | | 1975 | 53937 |
| ultrastructural study of mycoplasma pneumoniae in organ culture. | the ultrastructure of mycoplasma pneumoniae m129 was studied by using specialized staining methods for thin-section transmission electron microscopy. nucleic acid was shown in the cytoplasmic granules and fibrillar material in the nuclear region. the central filament of the highly structured tip contained basic protein. with one method of fixation, parallel filaments were seen in the central core. m. pneumoniae was enveloped in an extracellular mucoprotein layer that was especially concentrated ... | 1976 | 54354 |
| mycoplasma t strains and perinatal death. | in 23 out of 290 perinatal deaths in addis ababa, mycoplasma t strains were the only organisms responsible for congenital pneumonia and death. all but 2 of the infants were stillborn, 16 dying during the last six weeks of gestation. infections apparently occurred through intact fetal membranes. | 1976 | 54632 |
| [method of isolation and cultivation of mycoplasma]. | | 1975 | 56518 |
| serological studies of bovine mycoplasmas. ii. determination of taxonomical relatedness by means of seven tests. | | 1976 | 56834 |
| the isolation of carbon dioxide-requiring (carboxyphillic) type 19 pneumococcus (streptococcus pneumoniae) from diseased guinea-pigs. | type 19 pneumococcus was isolated only in anaerobic cultures from tissue fluids of guinea-pigs suffering from exudative pericarditis and pleuropneumonia. the organism was pathogenic to mice even after 40 subcultures. further study showed it to be carboxyphilic rather than anaerobic. the necessity of specifying various atmospheric conditions in the diagnostic bacteriological isolation routine is emphasized. | 1978 | 32435 |
| 2nd conference of the international organization for mycoplasmology (iom) august 28--september 1, 1978 in freiburg i. br. 1. symposium: "molecular biology of mycoplasmas". abstracts. | | 1978 | 31747 |
| mycoplasma in tissue culture: overview of detection methods. | mycoplasma contamination of tissue cultures is too costly, scientifically damaging and inappropriate, and perhaps, even medically hazardous to be ignored. prevention by continuous surveillance demands adequate mycoplasma testing procedures. despite a proliferation of indirect methods, the present consensus indicates that careful and extensive microbiological testing is still the cornerstone of any mycoplasma detection program. the finding of 'non-cultivable' or possible tissue-adapted micro-orga ... | 1976 | 57945 |
| adherence of mycoplasma gallisepticum to human erythrocytes. | pathogenic mycoplasmas adhere to and colonize the epithelial lining of the respiratory and genital tracts of infected animals. an experimental system suitable for the quantitative study of mycoplasma adherence has been developed by us. the system consists of human erythrocytes (rbc) and the avian pathogen mycoplasma gallisepticum, in which membrane lipids were labeled. the amount of mycoplasma cells attached to the rbc, which was determined according to radioactivity measurements, decreased on i ... | 1978 | 29007 |
| effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum (t-strain mycoplasma). | the effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum type viii was determined by using a well-buffered broth medium containing 10 mm urea. the addition of nh4cl to the medium at concentrations up to 10 mm did not affect growth; however, addition of larger quantities progressively decreased both the specific growth rate (mu) and the maximum yield of the culture, with concentrations of 80 mm completely inhibiting growth. addition of either 150 mm kcl or ... | 1978 | 26668 |
| an investigation into the viability of broth cultures of the t1 strain of mycoplasma mycoides sub-species mycoides. | the t1 broth vaccine against contagious bovine pleuropneumonia can only be stored for four weeks at +4 degrees c because after that time the titre of mycoplasma mycoides sub-species mycoides falls below the minimal vaccinating dose. an investigation into the death of these organisms during the stationary growth phase was made. the culture medium in which the vaccine was prepared was found to contain adequate nutrients. control of the ph by the addition of koh was shown to preserve the viability ... | 1978 | 24242 |
| [metabolism inhibition test for isolation of antibodies to mycoplasma pneumoniae]. | | 1976 | 61320 |
| infection and intracranial arterial thrombosis. | | 1976 | 61548 |
| [genital infections by viruses, mycoplasma and chlamydozoa (author's transl)]. | | 1976 | 61630 |
| oxygen uptake and lung function in mice infected with streptococcus pneumoniae, influenza virus, or mycoplasma pulmonis. | model systems of respiratory infection in mice were established with streptococcus pneumoniae, influenza virus, and mycoplasma pulmonis. the lt50 for s. pneumoniae was 2 1/2 days, for lethal influenza 6 days, and for m. pulmonis 5 days. morbidity in sublethal influenza infections reached a peak during days 5 to 10, with recovery indicated by the third week. the course of each pulmonary infection was followed by use of the animal's maximal ability to consume oxygen (vo2max by determining the weig ... | 1978 | 23401 |
| [identification of mycoplasma by the adsorption tests]. | | 1976 | 63605 |
| differential response of chlamydial and ureaplasma-associated urethritis to sulphafurazole (sulfisoxazole) and aminocyclitols. | 91 men with non-gonococcal urethritis (n.g.u.) were randomly treated with either sulphafurazole (sulfisoxazole), 500 mg orally q.i.d. for 10 days, or an aminocyclitol (streptomycin or spectinomycin), 2 g intramuscularity for 1 to 3 doses at 12 h intervals. initial urethral cultures were positive for chlamydia trachomatis (c) in 36 (40%). ureaplasma urealyticum (u) was isolated from the urethra or urine from20 (95%) of 21 white men in a first episode of n.g.u. who had negative chlamydia cultures. ... | 1976 | 63748 |
| problems related to the use of serum and trypsin in the growth of monkey kidney cells. | a function of serum in the growth medium for primary monkey kidney cells has been shown to be inhibition of proteolytic enzymes. serum inactivates the residual trypsin remaining from enzymatic digestion of the kidneys and the proteolytic enzymes subsequently synthesized by the cells. freshly trypsinized cells could be grown to monolayers in the absence of serum provided that they were repeatedly washed to remove residual trypsin. in the absence of serum, cell growth ceased on the 4-5th day after ... | 1976 | 22471 |
| characterization of membrane and cytoplasmic antigens of mycoplasma arginini by two-dimensional (crossed) immunoelectrophoresis. | two-dimensional immunoelectrophoresis was employed to electrophoretically identify membrane and cytoplasmic antigens of mycoplasma arginini g-230. five distinct cytoplasmic antigens were observed in soluble fractions prepared by digitonin lysis with electrophoretic mobilities (relative to bovine albumin) ranging from 0.36 to 0.86; four of these were common to other m. arginini strains: leonis and 23243. five membrane antigens were identified, two of which (0.4 and 0.2) were common to the other m ... | 1977 | 64457 |
| dna polymerase of mitochondria is a gamma-polymerase. | mitochondria isolated from rat liver cells or mycoplasma-free hela cells contain a single dna polymerase activity which is closely related to, or identical to, the dna polymerase gamma activity found in the homologous cell. in rat liver cells, about 16% of the total cytoplasmic gamma-polymerase activity is found associated with mitochondria and in hela cells about 20% of the total cellular gamma-polymerase is mitochondria associated. since mitochondria possess no unique dna polymerase activity, ... | 1977 | 16896 |
| morphology of ureaplasma urealyticum (t-mycoplasma) organisms and colonies. | the morphology of ureaplasm urealyticum in broth cultures was studied by phase-contrast microscopy. most organisms appeared singly or in pairs. long filaments and long chains of cocci, common in classical mycoplasma cultures, were not observed. on solid medium, u. urealyticum produced "fried-egg" colonies which developed according to the scheme suggested by razin and oliver (j. gen. microbiol., 1961) for the morphogenesis of the classical mycoplasma colonies. the formation of the peripheral zone ... | 1977 | 15986 |
| localization of enzymes in ureaplasma urealyticum (t-strain mycoplasma). | ureaplasma urealyticum cells were lysed by osmotic shock or by digitonin. the membrane fraction contained four to ten times as much protein as the cytoplasmic fraction. these values are in large excess of those reported for classical mycoplasmas, suggesting that the ureaplasma membrane fraction was heavily contaminated with proteins derived from the growth medium. the u. urealyticum urease activity was localized in the cytoplasmic fraction, whereas the adenosine triphosphatase activity was local ... | 1977 | 15980 |
| effects of carbon dioxide, urea, and ammonia on growth of ureaplasma urealyticum (t-strain mycoplasma). | by use of a simple device for continuous co2 gassing of ureaplasma urealyticum cultures growing in a liquid medium, we have been able to separate some of the effects of urea, co2, ammonia, and ph on growth. the co2 acted as a superior buffer in the ph range 5.7 to 6.8, which is optimal for ureaplasma growth. it was, therefore, possible to observe the effect of repeated additions of urea to the culture without alkalinization of the growth medium. we found that the repeated additions of urea did n ... | 1977 | 15979 |
| chlamydial and ureaplasma-associated urethritis. | | 1977 | 67307 |
| aetiology of acute epididymitis. | 24 patients with acute epididymitis were examined and underwent urethral and urine cultures for neisseria gonorrhoeae, ureaplasma urealyticum, herpes-simplex virus, chlamydia trachomatis, cytomegalovirus, and gram-negative aerobic bacteria. the results suggest that in young men the sexually transmitted organisms which cause urethritis (n. gonorrhoeae, c. trachomatis, and possibly u. urealyticum) may also lead to acute epididymitis, whereas in older men coliforms and pseudomonas are the predomina ... | 1977 | 67333 |
| [fluorescent test of mycoplasma colonies by agar diffusion technique (author's transl)]. | an agar diffusion test to directly stain mycoplasma colonies using a new fluorochrome 4,6-diamidino-2-phenyl-indol (dapi) is described. next to a colony in question a well is punched into the agar and filled with dapi. 30 min later the colonies in the diffusion zone show a light yellow - light green fluorescence. due to the high affinity of dapi to dna it is easily possible to distinguish between granular mycoplasma colonies and unspecific substances e.g. proteins and lipids from clinical materi ... | 1977 | 69747 |
| immunological analysis of plasma membranes of a t-strain of mycoplasma (ureaplasma urealyticum). | the cell membranes of a t-strain of mycoplasma, obtained by ultrasonic disruption, were as effective as whole organisms in eliciting metabolism-inhibiting and complement-fixing antibodies. the soluble fraction separated from cell membranes by centrifugation at 35,000 x g showed a minor ability to elicit an antibody response as measured by metabolism inhibition and complement fixation tests. after a further centrifugation at 100,000 x g, the immunogenic activity of the soluble fraction was comple ... | 1977 | 70405 |
| new fluorescent microscopical techinque in diagnostic microbiology. | | 1977 | 74007 |
| mucocutaneous reactions during mycoplasma pneumoniae infection. | | 1978 | 76182 |
| transformation of human embryo cells with the use of cell-free extracts of a human rhabdomyosarcoma cell line (hus-2): brief communication. | cell-free extracts of the human rhabdomyosarcoma cell line hus-2 caused the transformation of human embryo fibroblasts. this transformation included morphologic alteration, karyotypic change, and an increase in culture longevity. with the use of sex markers, multiple karyotypes confirmed that the human embryo fibroblasts were transformed, and the use of cell-free material further suggested the presence of a transforming virus. rna-dependent dna polymerase activity in a particle with a specific g ... | 1978 | 76683 |
| mucocutaneous reactions during mycoplasma pneumoniae infection. | | 1978 | 76914 |
| mucocutaneous reactions during mycoplasma pneumoniae infection. | | 1978 | 77406 |
| neotetrazolium staining of three avian mycoplasma serotypes for microagglutination test. | mycoplasma meleagridis, m. gallisepticum, and m. synoviae antigens were stained with neotetrazolium chloride for use in the microagglutination test. only the stained antigens of m. gallisepticum were acceptable in color intensity and test readability. | 1978 | 79413 |
| [method of isolation of mycoplasma from the microbial mixtures]. | | 1978 | 79692 |
| membrane-bound thioesterase activity in mycoplasmas. | thioesterase activity was found in all mycoplasmas tested. activity was highest in acholeplasma species, whereas most of the sterol-requiring mycoplasma species showed little activity. the thioesterase activity of acholoplasma laidlawii is confined to the cell membrane. the enzyme could not be released from the membrane by either low- or high-ionic-strength solutions, with or without ethylenediaminetetraacetic acid, nor solubilized by detergents. the enzyme has a general specificity for long-cha ... | 1977 | 14108 |
| identification of ureaplasma urealyticum (t-strain mycoplasma) in patient with polyarthritis. | a 10-year-old boy with confirmed congenital agammaglobulinaemia presented with polyarthritis while on gammaglobulin replacement therapy. initial cultures of material aspirated from an abscess and of joint fluid were negative, and symptoms progressed despite antibiotic therapy. synovial-biopsy material, cultured specifically for mycoplasmas, was positive for ureaplasma urealyticum as were the blood, abscess fluids, throat-swab, and nasopharyngeal secretions. therapy, based on in-vitro studies of ... | 1978 | 81929 |
| examination of sputum in legionnaires' disease. | | 1978 | 82006 |
| thermoplasma acidophilum: intracellular ph and potassium concentration. | thermoplasma acidophilum is a free-living thermophilic mycoplasma. although the organism lacks a cell wall, it can grow in medium as dilute as 66 mosm. the intracellular k+ concentration can be as low as 17 mm, but varies according to the osmolality of the culture medium. the internal ph can be measured by taking advantage of the fact that t. acidophilum undergoes lysis when the ph is adjusted to neutrality. thus, by appropriate analysis of titration curves, it is possible to conclude that the i ... | 1976 | 12804 |
| microcalorimetric detection of growth of mycoplasmatales. | a static ampoule microcalorimeter was used to study the growth of mycoplasmas, acholeplasmas and ureaplasmas. growth as indicated by thermograms was compared with the results of conventional methods, namely, terminal dilution counts, plate counts, turbidimetric measurements, glucose consumption and ph changes. removal of oxygen had little effect on mycoplasma growth. the microcalorimetric method is potentially useful for identifying and enumerating the members of the mycoplasmatales. | 1976 | 11273 |
| eperythrozoon ovis. i. serological diagnosis of infection by the indirect immunoflurescent antibody test. | the indirect immunofluorescent antibody test (ifat) was evaluated for the detection of eperythrozoon ovis infection in sheep. the test first became positive, on the average, 10 days following exposure to e. ovis and remained positive for 24 months, the longest period checked. reciprocal titres in the first three days after becoming positive in the ifat were between 4 and 80, but rose to 640 within 9 weeks; in general titres were between 4 and 640, exceptionally titres of 1280 were obtained. nega ... | 1978 | 83044 |
| uptake, transbilayer distribution, and movement of cholesterol in growing mycoplasma capricolum cells. | | 1978 | 83152 |
| cold hemagglutinin cross-reactivity with mycoplasma pneumoniae. | convalescent sera from proven cases of infection with mycoplasma pneumoniae, and rabbit antisera to m. pneumoniae and to human erythrocyte glycoprotein contained cold hemagglutinins which were reactive only for human erythrocytes. only the human serum cold agglutinins were inhibited by soluble integral glycoproteins derived from human erythrocyte ghosts by treatment with chloroform-methanol. rabbit antiserum to chloroform-methanol glycoprotein, as well as to m. pneumoniae, fixed complement with ... | 1978 | 83298 |
| evidence for a ruthenium red-staining extracellular layer as the haemagglutinin of the wvu 1853 strain of mycoplasma synoviae. | | 1978 | 84665 |
| detection of serum proteins in the electrophoretic patterns of total proteins of mycoplasma cells. | the contamination of mycoplasma cell preparations by serum proteins originating from culture medium was studied. a. laidlawii and m. arthritidis cells were grown in the presence of 14c-aminoacids, and the cells were washed with 0-9% nac1 by threefold centrifugation. total proteins of the washed cells were analysed by sds gel electrophoresis. coomassie-stained electrophoretic patterns were compared with autoradiographs of the same gels. the stained electrophoretic pattern of washed a. laidlawii g ... | 1976 | 10333 |