| [bioinformatics analysis of co i gene of simulium quinquestriatum]. | the genomic dna was extracted from simulium quinquestriatum (sq) and its co i gene was amplified by pcr. the pcr product was purified and cloned into plasmid pmd18-t vector. the recombinant plasmid was transformed into escherichia coli dh5alpha and then identified by digestion with restriction enzyme and pcr amplification. the amplified fractions (1 621 bp) included complete co i gene (1 542 bp, genbank accession number: dq534949), 5' trna-tyr and 3' trna-leu partial fraction. the co i gene sequ ... | 2010 | 21500540 |