| ramie: its potentialities as a vascular prosthetic graft preliminary report of an experimental appraisal. | | 1966 | 5915023 |
| arg²³⁵ is an essential catalytic residue of bacillus pumilus dks1 pectate lyase to degum ramie fibre. | after 24 h of incubation with only purified pectate lyase isolated from bacillus pumilus dks1 (ef467045), the weight loss of the ramie fibre was found to be 25%. to know the catalytic residue of pectate lyase the pel gene encoding a pectate lyase from the strain bacillus pumilus dks1 was cloned in e. coli xl1blue and expressed in e. coli bl21 (de3) plyss. the pel gene was sequenced and showed 1032 bp length. after purification using cm-sepharose the enzyme showed molecular weight of 35 kda and m ... | 2011 | 20596756 |
| lignin variability in plant cell walls: contribution of new models. | lignin is a major component of certain plant cell walls. the enzymes and corresponding genes associated with the metabolic pathway leading to the production of this complex phenolic polymer have been studied for many years now and are relatively well characterized. the use of genetically modified model plants (arabidopsis, tobacco, poplar.) and mutants has contributed greatly to our current understanding of this process. the recent utilisation and/or development of a number of dedicated genomic ... | 2011 | 21889043 |
| enhancing production of alkaline polygalacturonate lyase from bacillus subtilis by fed-batch fermentation. | alkaline polygalacturonate lyase (pgl, ec 4.2.2.2) is an enzyme used in many industries. we developed a fed-batch fermentation process that combines the enzymatic pretreatment of the carbon source with controlling the ph of the fermentative broth to enhance the pgl production from bacillus subtilis 7-3-3 to decrease the production cost. maintaining the fermentation broth at ph 6.5 prior to feeding with ammonia and at ph 6.0 after feeding significantly improved pgl activity (743.5 u ml-1) compare ... | 2014 | 24603713 |
| transgenic plants of ramie (boehmeria nivea gaud.) obtained by agrobacterium mediated transformation. | a regeneration and transformation protocol for ramie (boehmeria nivea gaud.) is presented. regeneration was obtained from leaf discs placed on solid b-5 medium (gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. co-cultivation of leaf discs with agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by southern blot and analysis of expression of the gus-marker gene. | 1993 | 24201876 |
| improvement of degummed ramie fiber properties upon treatment with cellulase secreting immobilized a. larrymoorei a1. | degummed ramie fiber is widely used in the textile industry. cellulase enzyme can be effectively used for bio-polishing of the ramie fiber. we immobilized agrobacterium larrymoorei a1, a potent extra-cellular cellulase producing bacteria, in ca-alginate. the production of enzyme significantly increased with increasing alginate concentration and reached a maximum activity of 0.28 iu/ml at 20 g/l, which was 32% higher as compared to free cells. these immobilized cells were used on ramie fibers. sc ... | 2015 | 25194464 |
| directed evolution and structural analysis of alkaline pectate lyase from the alkaliphilic bacterium bacillus sp. strain n16-5 to improve its thermostability for efficient ramie degumming. | thermostable alkaline pectate lyases have potential applications in the textile industry as an alternative to chemical-based ramie degumming processes. in particular, the alkaline pectate lyase from bacillus sp. strain n16-5 (bsppela) has potential for enzymatic ramie degumming because of its high specific activity under extremely alkaline conditions without the requirement for additional ca(2+). however, bsppela displays poor thermostability and is inactive after incubation at 50°c for only 30 ... | 2015 | 26070675 |
| cloning, expression and characterization of a pectate lyase from paenibacillus sp. 0602 in recombinant escherichia coli. | biotechnological applications of microbial pectate lyases (pels) in plant fiber processing are considered as environmentally friendly. as such, they become promising substitutes for conventional chemical degumming process. since applications of pels in various fields are widening, it is necessary to explore new pectolytic microorganisms and enzymes for efficient and effective usage. here, we describe the cloning, expression, characterization and application of the recombinant pel protein from a ... | 2014 | 24612647 |
| the alkaline pectate lyase pel168 of bacillus subtilis heterologously expressed in pichia pastoris is more stable and efficient for degumming ramie fiber. | the conventional degumming process of ramie with alkaline treatment at high temperature causes severe environmental pollution. pectate lyases can be used to remove pectin from ramie in a degumming process with reduced environmental pollution and energy consumption. pectate lyase pel168 from bacillus subtilis has been previously characterized and the protein structure was resolved. however, bacillus is not a suitable host for pectate lyases during the degumming process since most bacillus produce ... | 2013 | 23510095 |
| characteristics of polygalacturonate lyase c from bacillus subtilis 7-3-3 and its synergistic action with pela in enzymatic degumming. | an alkaline polygalacturonate lyase (pgl) from bacillus subtilis 7-3-3, pelc, with diverse depolymerization abilities for different pectin substrates was found. the pgl activity of pelc decreased with increasing degree of methyl esterification of the substrate. pela and pelc displayed notable synergistic effects in the enzymatic degumming of ramie fibers. gum loss rates increased by 62% when pelc was used to replace up to three-eighths of the pela dose (pelc, 60 u g(-1) ramie fibers). to the bes ... | 2013 | 24236123 |
| the effects of boehmeria nivea (l.) gaud. on embryonic development: in vivo and in vitro studies. | boehmeria nivea (l.) gaud. was commonly used to treat miscarriages clinically. the aim of this study was to examine its safety for embryonic development. | 2010 | 21195153 |
| molecular characterization of ramie mosaic virus isolates detected in jiangsu and zhejiang provinces, china. | virus isolates were obtained from three ramie samples (boehmeria nivea l.) showing yellow mosaic symptoms collected in jiangsu and zhejiang provinces, china. comparison of partial dna-a fragments amplified with begomovirus universal primers pa/pb revealed that these viral isolates shared a high sequence identity. the complete dna-a sequences of two isolates j4 and z1 were determined to be 2736 and 2737 nts, respectively, sharing 94.7% nucleotide sequence identity with each other. also, the dna-b ... | 2010 | 20822317 |
| constitutional tolerance to heavy metals of a fiber crop, ramie (boehmeria nivea), and its potential usage. | it is observed that ramie (boehmeria nivea), an economic fiber crop, can establish and colonize metal-contaminated sites in china. metal tolerance and accumulation by ramie originating from 13 metal-contaminated and 4 "clean" sites in china were compared under field and hydroponic conditions. all selected populations and germplasms displayed good growth performance under diverse metal-contaminated habitats; while growth responses, metal accumulation and tolerance were similar among the 8 populat ... | 2010 | 19828218 |
| retraction. transgenic ramie [boehmeria nivea (l.) gaud.]: factors affecting the efficiency of agrobacterium tumefaciens-mediated transformation and regeneration. | | 2009 | 19774385 |
| transgenic ramie [boehmeria nivea (l.) gaud.]: factors affecting the efficiency of agrobacterium tumefaciens-mediated transformation and regeneration. | in the present study, an efficient agrobacterium-mediated gene transformation system was developed for ramie [boehmeria nivea (l.) gaud.] based on the examinations of several factors affecting plant transformation efficiency. the effects of agrobacterium cell density, acetosyringone, co-cultivation temperature, co-cultivation duration, co-cultivation photoperiod and ph on stable transformation were evaluated. agrobacterium at a concentration of od = 0.5-0.8 improved the efficiency of transformat ... | 2009 | 19533144 |
| nematode problems affecting agriculture in the philippines. | nematodes are considered major pests on most economic crops in the philippines, particularly on banana, pineapple, citrus, tomato, ramie, and sugarcane. radopholus similis is the most destructive nematode on banana, while meloidogyne spp. are more serious on various vegetable crops such as tomato, okra, and celery and on fiber crops such as ramie. tylenchulus semipenetrans is a problem on citrus and rotylenchulus reniformis on pineapple and some legume crops. hirschmanniella oryzae and aphelench ... | 1988 | 19290204 |
| involvement of grp78 in inhibition of hbv secretion by boehmeria nivea extract in human hepg2 2.2.15 cells. | previous studies showed that the root extract of boehmeria nivea (bne) can significantly suppress the production of hepatitis b virus (hbv) in vitro and in vivo. in this study, viral core and large-surface proteins accompanied with their encapsidated viral dna were observed to accumulate within the cells. notably, 78-kda glucose-regulated protein (grp78) was found to be suppressed by bne, and stimulation of the grp78 expression by thapsigargin could rescue virus production initially inhibited by ... | 2009 | 19228285 |
| large-scale degumming of ramie fibre using a newly isolated bacillus pumilus dks1 with high pectate lyase activity. | a combined (enzymatic and chemical) process using a bacillus pumilus strain (dks1), isolated from the soil, was used to degum ramie bast fibres. after 24 h of incubation with the isolated pectinolytic strain using a low-cost medium, the weight loss of the ramie fibre was found to be 25% under small scale. high activity of pectate lyase was detected in the culture supernatants; 400 kg of ramie fibres was degummed with 24% weight loss in large-scale degumming under field conditions. no cellulase a ... | 2009 | 18956216 |
| the anti-hepatitis b virus activity of boehmeria nivea extract in hbv-viremia scid mice. | boehmeria nivea extract (bne) is widely used in southern taiwan as a folk medicine for hepato-protection and hepatitis treatment. in previous studies, we demonstrated that bne could reduce the supernatant hepatitis b virus (hbv) dna in hbv-producing hepg2 2.2.15 cells. in the present study, we established an animal model of hbv viremia and used it to validate the efficacy of bne in vivo. in this animal model, serum hbv dna and hbsag were elevated in accordance with tumor growth. to evaluate the ... | 2010 | 18955304 |
| enzymes in bast fibrous plant processing. | the program cost action 847 textile quality and biotechnology (2000-2005) has given an excellent chance to review the possibilities of the research, aiming at development of the industrial application of enzymes for bast fibrous plant degumming and primary processing. the recent advancements in enzymatic processing of bast fibrous plants (flax, hemp, jute, ramie and alike plants) and related textiles are given. the performance of enzymes in degumming, modification of bast fibres, roving, yarn, r ... | 2006 | 16791732 |
| a marked enhancement in the production of a highly alkaline and thermostable pectinase by bacillus pumilus dcsr1 in submerged fermentation by using statistical methods. | the production of a highly alkaline and thermostable pectinase of bacillus pumilus was optimized in submerged fermentation using plackett-burman design and response surface methodology. three fermentation variables (c:n ratio, k(2)hpo(4), and ph), which were identified to significantly affect pectinase production by plackett-burman design were further optimized using response surface methodology of central composite design (ccd). an over all 34- and 41-fold increase in enzyme production was achi ... | 2006 | 15936940 |
| [study on the production of beta-mannanase by bacillus m50]. | bacillus sp. m50 produced beta-mannanase efficiently in the medium composed of 4% konjac powder, 1.0% (nh4)2so4 and 0.35% na2co3. fermentation of beta-mannanase by bacillus sp. m50 has been carried out in 100 l tank. the temperature, ventitation quantity and agitation speed were 30-32 degrees c, 1:0.75 vvm and 220 r/min respectively. the fermentation cycle was 28 h and the highest beta-mannanase activity was 330 u/ml. the optimal temperature and ph for beta-mannanase reaction were 50 degrees c a ... | 2000 | 12548880 |
| cp/mas (13)c nmr study of cellulose and cellulose derivatives. 2. complete assignment of the (13)c resonance for the ring carbons of cellulose triacetate polymorphs. | complex ring (13)c resonance lines of the cross-polarization/magic angle spinning (cp/mas) (13)c nmr spectra of cellulose triacetate (cta) i and cta ii were completely assigned, for the first time, by (13)c-enriched cta allomorphs. the (13)c-enriched cta i was prepared by heterogeneous acetylation of bacterial cellulose which was biosynthesized by acetobacter xylinum (a. xylinum) atcc10245 from culture medium containing d-(2-(13)c)-, d-(3-(13)c)-, or d-(5-(13)c)glucose as a carbon source, while ... | 2002 | 12071761 |
| degumming of ramie fibers by alkalophilic bacteria and their polysaccharide-degrading enzymes. | three strains of alkalophilic bacteria, bacillus sp. nt-39, nt-53 and nt-76, were selected for the degumming of ramie fibers and production of polysaccharide-degrading enzymes. after 48 h of incubation with the strains, the loss of the gum might amount to 5.0% or more of the fibers and a number of polysaccharide-degrading enzymes were secreted to the culture supernatants. the residual gum of the fibers decreased to 9.4% after 5 h of enzymatic degumming. analysis of gum contents and enzyme activi ... | 2001 | 11265793 |
| cloning, sequencing and expression of the xylanase gene from a bacillus subtilis strain b10 in escherichia coli. | bacillus subtilis strain b10 was isolated for degumming of ramie blast fibers, and a fragment of 642-bp was amplified from chromosomal dna by using primers directed against the sequence of bacillus subtilis xylanase gene given in genbank. the positive clones were screened on the selected lb agar plates supplemented with xylan by congo-red staining method. the recombinant plasmid from one positive clone was used for further analysis and dna sequencing. the gene sequence is different from the repo ... | 2006 | 15951169 |
| inhibition of hepatitis b virus production by boehmeria nivea root extract in hepg2 2.2.15 cells. | to explore the anti-hepatitis b virus (hbv) effects of boehmeria nivea (b. nivea) root extract (bne) by using the hepg2 2.2.15 cell model system. | 2006 | 17007029 |
| evaluation of the hepatoprotective and antioxidant activity of boehmeria nivea var. nivea and b. nivea var. tenacissima. | in this study, the relationship between liver protective effects and antioxidant activity of boehmeria nivea var. nivea (= b. nivea) and b. nivea var. tenacissima (= b. frutescens) was investigated. the water extracts of both plants exhibited a hepatoprotective activity against ccl4-induced liver injury. b. nivea var. nivea and b. nivea var. tenacissima, also showed anti-oxidant effects in fecl2-ascorbate induced lipid peroxidation in rat liver homogenate. moreover, the active oxygen species sca ... | 1998 | 9533427 |
| purification and characterization of an extracellular pectate lyase from an amycolata sp. | the extracellular pectate lyase (ec 4.2.2.2) of a nonsporulating amycolata sp. was purified to homogeneity by anion- and cation-exchange chromatographies followed by hydrophobic interaction chromatography. the enzyme cleaved polygalacturonate but not highly esterified pectin in a random endolytic transeliminative mechanism that led to the formation of a wide range of 4,5-unsaturated oligogalacturonates. as shown by high-performance anion-exchange chromatography and pulsed amperometric detection, ... | 1995 | 7486993 |
| cloning, evaluation, and high-level expression of a thermo-alkaline pectate lyase from alkaliphilic bacillus clausii with potential in ramie degumming. | alkaline pectate lyases (pels) have potential application in bioscouring of the textile industry. in this study, a thermo-alkaline pel (bacpela) gene from an alkaliphilic bacillus clausii strain was cloned and overexpressed in escherichia coli. the mature bacpela exhibited maximum activity at ph 10.5 and 70 °c and showed high cleavage capability on methylated pectins. bacpela showed the highest specific activity of 936.2 u mg(-1) on ≥85% methylated pectin and 675.5 u mg(-1) on standard substrate ... | 2017 | 28184988 |
| enzymatic degumming of ramie bast fibers. | bast fibers from ramie (boehmeria nivea) were treated with cell-free culture supernatants from an amycolata sp. and a recombinant streptomyces lividans strain expressing the amycolata pectate lyase to investigate the degumming effects of different extracellular polysaccharide-degrading enzymes. culture supernatants from the amycolata sp. with high pectate lyase activities were most effective in fiber separation and reduced the gum content of ramie fibers by 30% within 15 h. xylanase activity pro ... | 2000 | 10784295 |
| degumming and characterization of ramie fibre using pectate lyase from immobilized bacillus pumilus dks1. | the present study was aimed at finding the optimal conditions for the production of pectate lyase using immobilized bacillus pumilus dks1 cells in calcium-alginate (ca-alginate) beads and determining the efficient degumming of ramie fibre. | 2009 | 19416461 |
| degumming of ramie fiber and the production of reducing sugars from waste peels using nanoparticle supplemented pectate lyase. | banana, citrus and potato peels were subjected to treatment with hydroxyapatite nanoparticle (np) supplemented purified pectate lyase (np-pl), isolated from bacillus megaterium ak2 to produce reducing sugar (rs). at both 50 and 90°c production of rs by np-pl was almost twofold greater than that by untreated pectate lyase (pl) from each of the three peels. the optimal production of rs from banana and citrus peels were after 24 and 6h of incubation while it was 24 and 4h for potato peels at 50 and ... | 2013 | 23587821 |
| improving the thermoactivity and thermostability of pectate lyase from bacillus pumilus for ramie degumming. | thermostable alkaline pectate lyases can be potentially used for enzymatically degumming ramie in an environmentally sustainable manner and as an alternative to the currently used chemical-based ramie degumming processes. to assess its potential applications, pectate lyase from bacillus pumilus (atcc 7061) was cloned and expressed in escherichia coli. evolutionary strategies were applied to generate efficient ramie degumming enzymes. obtained from site-saturation mutagenesis and random mutagenes ... | 2015 | 25287558 |
| effects of different substrates on lignocellulosic enzyme expression, enzyme activity, substrate utilization and biological efficiency of pleurotus eryngii. | pleurotus eryngii is one of the most valued and delicious mushrooms which are commercially cultivated on various agro-wastes. how different substrates affect lignocellulosic biomass degradation, lignocellulosic enzyme production and biological efficiency in pleurotus eryngii was unclear. | 2016 | 27607466 |
| draft genome sequence of an industrially important bacillus sp. from mandarmani coastal waters in midnapur district, west bengal, india. | reported here is the draft genome sequence of an amylase-, protease-, dnase-, oxidase-, gelatinase-, and catalase-producing, gram-positive diplobacillus (bacillus sp. sm1 strain mcc2138), which was isolated from marine coastal waters and has the ability to degum raw silk fabric as well as ramie fiber. the genome comprises 1.76 mb with a gc content of 34.5%. | 2016 | 27540077 |
| ramie leaf extracts suppresses adipogenic differentiation in 3t3-l1 cells and pig preadipocytes. | the present study was carried out to evaluate the anti-obesity effect of different concentrations of extracts of hot air-dried ramie leaf (hr) and freeze-dried ramie leaf (fr) in 3t3-l1 cells and pig preadipocytes. to analyze the effect on cell proliferation, cells were treated with 25 μg/ml or 100 μg/ml hr or fr extract for 2 days. cell differentiation was evaluated by measuring glycerol-3-phosphate dehydrogenase and lipoprotein lipase (lpl) activities and intracellular triglyceride content. tr ... | 2016 | 26954122 |
| investigation of the adsorption-reduction mechanisms of hexavalent chromium by ramie biochars of different pyrolytic temperatures. | to investigate the relationship between cr(vi) adsorption mechanisms and physio-chemical properties of biochar, ramie residues were oxygen-limited pyrolyzed under temperature varying from 300 to 600°c. batch adsorption experiments indicated that higher pyrolysis temperature limits cr(vi) sorption in terms of capacity and affinity due to a higher aromatic structure and fewer polar functional groups in biochar. both electrostatic (physical) and ionic (chemical) interactions were involved in the cr ... | 2016 | 27376834 |
| characterization by suppression subtractive hybridization of transcripts that are differentially expressed in leaves of anthracnose-resistant ramie cultivar. | for the purpose of screening putative anthracnose resistance-related genes of ramie (boehmeria nivea l. gaud), a cdna library was constructed by suppression subtractive hybridization using anthracnose-resistant cultivar huazhu no. 4. the cdnas from huazhu no. 4, which were infected with colletotrichum gloeosporioides, were used as the tester and cdnas from uninfected huazhu no. 4 as the driver. sequencing analysis and homology searching showed that these clones represented 132 single genes, whic ... | 2012 | 24415836 |
| fiber from ramie plant (boehmeria nivea): a novel suture biomaterial. | the quest for developing an ideal suture material prompted our interest to develop a novel suture with advantageous characters to market available ones. from natural origin only silk, cotton and linen fibers are presently available in market as non-absorbable suture biomaterials. in this study, we have developed a novel, cost-effective, and biocompatible suture biomaterial from ramie plant, boehmeria nivea fiber. field emission scanning electron microscopy (fe-sem), energy-dispersive x-ray spect ... | 2016 | 26952488 |
| large-scale developing of simple sequence repeat markers and probing its correlation with ramie (boehmeria nivea l.) fiber quality. | marker-assisted selection is an important component of the discipline of molecular breeding. using dna markers to assist in plant breeding, the efficiency and precision could be greatly increased. however, the scarcity number of identified dna markers has hindered the research and the breeding process of ramie (boehmeria nivea l.) in many aspects, especially fiber quality, one of the top-priority breeding objectives of ramie. in this study, 4230 ssr loci were identified in 3969 unigenes (6.80 % ... | 2016 | 26577947 |
| biotechnological potential of agro residues for economical production of thermoalkali-stable pectinase by bacillus pumilus dcsr1 by solid-state fermentation and its efficacy in the treatment of ramie fibres. | the production of a thermostable and highly alkaline pectinase by bacillus pumilus dcsr1 was optimized in solid-state fermentation (ssf) and the impact of various treatments (chemical, enzymatic, and in combination) on the quality of ramie fibres was investigated. maximum enzyme titer (348.0 ± 11.8 ug(-1) dbb) in ssf was attained, when a mixture of agro-residues (sesame oilseed cake, wheat bran, and citrus pectin, 1 : 1 : 0.01) was moistened with mineral salt solution (a(w) 0.92, ph 9.0) at a su ... | 2012 | 22928091 |
| robot-assisted minimally invasive esophagectomy for esophageal cancer: a systematic review. | this paper describes the technique of robot-assisted minimally invasive esophagectomy. (ramie) also, a systematic literature search was performed. safety and feasibility of ramie was demonstrated in all reports. short term oncologic results show radical resection rates of 77-100% and 18-43 lymph nodes harvested. ramie offers great visualization of the mediastinum and enables meticulous dissection in the mediastinum from diaphragm to thoracic inlet. | 2015 | 26390285 |
| functional application of noble metal nanoparticles in situ synthesized on ramie fibers. | different functions were imparted to ramie fibers through treatment with noble metal nanoparticles including silver and gold nanoparticles. the in situ synthesis of silver and gold nanoparticles was achieved by heating in the presence of ramie fibers in the corresponding solutions of precursors. the unique optical property of synthesized noble metal nanoparticles, i.e., localized surface plasmon resonance, endowed ramie fibers with bright colors. color strength (k/s) of fibers increased with hea ... | 2015 | 26383541 |
| high-resolution bacterial growth inhibition profiling combined with hplc-hrms-spe-nmr for identification of antibacterial constituents in chinese plants used to treat snakebites. | bacterial infection is one of the main secondary infections caused by snakebite. the 88 plant species investigated in this study have been used as folk remedies for treatment of snakebite, and it is therefore the aim of this study to investigate whether the plants contain compounds with bacterial growth inhibition. | 2014 | 25043779 |
| anti-hepatitis b virus activity of boehmeria nivea leaf extracts in human hepg2.2.15 cells. | boehmeria nivea (linn.) gaudich of the urticaceae family is a perennial ratoon herbal plant, the root of which is used in traditional chinese medicine and possesses a variety of pharmacological properties. the 20% ethanol boehmeria nivea root extract was shown to exert an anti-hepatitis b virus (hbv) effect in vitro and in vivo; however, whether the boehmeria nivea leaf (bnl) extract possesses similar properties has not been determined. in this study, we aimed to investigate the anti-hbv effects ... | 2014 | 24649087 |
| removal of copper from acid wastewater of bioleaching by adsorption onto ramie residue and uptake by trichoderma viride. | a continuous batch bioleaching was built to realize the bioleaching of sewage sludge in large scale. in the treatment, heavy metal in acid wastewater of bioleaching was removed by adsorption onto ramie residue. then, acid wastewater was reused in next bioleaching batch. in this way, most time and water of bioleaching was saved and leaching efficiency of copper, lead and chromium kept at a high level in continuous batch bioleaching. it was found that residual heavy metal in sewage sludge is highl ... | 2013 | 23567687 |
| development of a core collection for ramie by heuristic search based on ssr markers. | there are more than 2000 ramie germplasms in the national ramie germplasm nursery affiliated with the institute of bast fiber crops, chinese academy of agricultural science, china. as it is difficult to perform effective conservation, management, evaluation, and utilization of redundant genetic resources, it is necessary to construct a core collection by using molecular markers. in this study, a core collection of ramie consisting of 22 germplasms was constructed from 108 accessions by heuristic ... | 2014 | 26019563 |
| isolation, identification, and environmental adaptability of heavy-metal-resistant bacteria from ramie rhizosphere soil around mine refinery. | six bacteria strains from heavy-metal-polluted ramie rhizosphere soil were isolated through cd(2+) stress, which were numbered as jj1, jj2, jj10, jj11, jj15, and jj18. sequence alignment and phylogenic analysis showed that strain jj1 belonged to pseudomonas, strain jj2 belonged to cupriavidus, strains jj11 and jj15 belonged to bacillus, and strains jj10 and jj18 belonged to acinetobacter. the tolerance capability of all the strains was the trend of pb(2+) > zn(2+) > cu(2+) > cd(2+), the maximum ... | 2017 | 28391469 |
| modeling of the morphological change of cellulose microfibrils caused with aqueous naoh solution: the longitudinal contraction and laterally swelling during decrystallization. | the conformation of cellulose microfibrils treated with aqueous naoh was modeled as partially decrystallized cellulose chains before completing conversion to cellulose ii, in order to elucidate the change in morphology of ramie fiber caused by naoh treatment. equations for the relative length and width of the microfibrils were derived on the basis of partially decrystallized microfibrils modeling. each equation contains four parameters, n, β, w c , and c r , which correspond to the number of glu ... | 2017 | 28332081 |
| preservation of replaced left hepatic artery during robotic-assisted minimally invasive esophagectomy: a case series. | finding of a significant replaced left hepatic artery (rlha) during esophagectomy is relatively rare, with an incidence of approximately 5%. sparing of the artery may be required to avoid complications of liver ischemia. robotic assistance during esophagectomy may provide a technically superior method of artery preservation with minimally invasive approaches. | 2017 | 28251793 |
| classification and identification of plant fibrous material with different species using near infrared technique-a new way to approach determining biomass properties accurately within different species. | plant fibrous material is a good resource in textile and other industries. normally, several kinds of plant fibrous materials used in one process are needed to be identified and characterized in advance. it is easy to identify them when they are in raw condition. however, most of the materials are semi products which are ground, rotted or pre-hydrolyzed. to classify these samples which include different species with high accuracy is a big challenge. in this research, both qualitative and quantit ... | 2016 | 28105037 |
| structure-based engineering of a pectate lyase with improved specific activity for ramie degumming. | biotechnological applications of microbial pectate lyases (pels) in plant fiber processing are promising, eco-friendly substitutes for conventional chemical degumming processes. however, to potentiate the enzymes' use for industrial applications, resolving the molecular structure to elucidate catalytic mechanisms becomes necessary. in this manuscript, we report the high resolution (1.45 å) crystal structure of pectate lyase (peln) from paenibacillus sp. 0602 in apo form. through sequence alignme ... | 2017 | 28028551 |
| a ramie bzip transcription factor bnbzip2 is involved in drought, salt, and heavy metal stress response. | bzip transcription factors play key roles in plant growth, development, and stress signaling. a bzip gene bnbzip2 (genbank accession number: kp642148) was cloned from ramie. bnbzip2 has a 1416 base pair open reading frame, encoding a 471 amino acid protein containing a characteristic bzip domain and a leucine zipper. bnbzip2 shares high sequence similarity with bzip factors from other plants. the bnbzip2 protein is localized to both nuclei and cytoplasm. transcripts of bnbzip2 were found in vari ... | 2016 | 27845851 |
| biochar pyrolyzed from mgal-layered double hydroxides pre-coated ramie biomass (boehmeria nivea (l.) gaud.): characterization and application for crystal violet removal. | a novel biochar/mgal-layered double hydroxides composite (cb-ldh) was prepared for the removal of crystal violet from aqueous solution by pyrolyzing mgal-ldh pre-coated ramie stem (boehmeria nivea (l.) gaud.). pyrolysis played dual role for both converting biomass into biochar and calcining mgal-ldh during the pyrolysis process. scanning electron microscopy (sem), transmission electron microscopy (tem), energy-dispersive x-ray analysis (eds), x-ray photoelectron spectroscopy (xps), fourier trans ... | 2016 | 27591848 |
| isobaric tags for relative and absolute quantitation (itraq)-based comparative proteome analysis of the response of ramie under drought stress. | in this study, we conducted the first isobaric tags for relative and absolute quantitation (isobaric tags for relative and absolute quantitation (itraq))-based comparative proteomic analysis of ramie plantlets after 0 (minor drought stress), 24 (moderate drought stress), and 72 h (severe drought stress) of treatment with 15% (w/v) poly (ethylene glycol)6000 (peg6000) to simulate drought stress. in our study, the association analysis of proteins and transcript expression revealed 1244 and 968 ass ... | 2016 | 27689998 |
| [short-term outcomes of robot-assisted minimally invasive esophagectomy for esophageal cancer]. | to evaluate the feasibility, safety and short-term clinical outcomes of robot-assisted minimally invasive esophagectomy (ramie). | 2016 | 27680066 |
| attaining proficiency in robotic-assisted minimally invasive esophagectomy while maximizing safety during procedure development. | robotic-assisted minimally invasive esophagectomy (ramie) is an emerging complex operation with limited reports detailing morbidity, mortality, and requirements for attaining proficiency. our objective was to develop a standardized ramie technique, evaluate procedure safety, and assess outcomes using a dedicated operative team and 2-surgeon approach. | 2016 | 27662372 |
| [cd runoff load and soil profile movement after implementation of some typical contaminated agricultural soil remediation strategies]. | owing to the strong ability to immobilize and hyperaccumulate some toxic heavy metals in contaminated soils, the biochar, lime and such as hyperaccumulator ramie received increasing interests from crops and environment safety in recent years. outdoor pot experiment was conducted to compare the impacts of lime and biochar addition in paddy rice treatment, hyperaccumulator ramie and ramie combined with edta of plant phytoremediation methods on soil available cd dynamics in rainfall runoff and the ... | 2016 | 27363167 |
| transcriptome analysis of ramie (boehmeria nivea l. gaud.) in response to ramie moth (cocytodes coerulea guenée) infestation. | the ramie moth cocytodes coerulea guenée (rm) is an economically important pest that seriously impairs the yield of ramie, an important natural fiber crop. the molecular mechanisms that underlie the ramie-pest interactions are unclear up to date. therefore, a transcriptome profiling analysis would aid in understanding the ramie defense mechanisms against rm. in this study, we first constructed two cdna libraries derived from rm-challenged (ch) and unchallenged (ck) ramie leaves. the subsequent s ... | 2016 | 27034936 |
| biochemical and metabolic changes in arsenic contaminated boehmeria nivea l. | arsenic (as) is identified by the epa as the third highest toxic inorganic contaminant. almost every 9th or 10th human in more than 70 countries including mainland china is affected by as. arsenic along with other toxins not only affects human life but also creates alarming situations such as the deterioration of farm lands and desertion of industrial/mining lands. researchers and administrators have agreed to opt for phytoremediation of as over costly cleanups. boehmeria nivea l. can soak up va ... | 2016 | 27022603 |
| identification of small auxin-up rna (saur) genes in urticales plants: mulberry (morus notabilis), hemp (cannabis sativa) and ramie (boehmeria nivea). | small auxin-up rna (saur) genes are important gene families in auxin signalling transduction and are commonly used as early auxin responsive markers. till date, no saur gene is identified in urticales plants despite of the published bioinformation of mulberry, hemp and ramie. in this study, we used arabidopsis sequences as query to search against mulberry, hemp genomes and ramie transcriptome database. in total, we obtained 62, 56 and 71 saur genes in mulberry, hemp and ramie, respectively. phyl ... | 2016 | 27019439 |
| recycling agriculture wastes of ramie stalk as bioadsorbents for cd(2+) removal: a kinetic and thermodynamic study. | in this study, we exhibit the recycling of agriculture wastes of ramie stalk as bioadsorbents for cd(2+) removal. based on our experimental results, it is realized that cd(2+) adsorption to ramie stalk is highly ph sensitive, indicating the adsorption is driven by surface complexation reaction. the high adsorption capacity of ramie stalk toward cd(2+) (qm = 10.33 mg g(-1), 0.09 mol-cd g(-1)), which corresponds to around 21.95% of active adsorption sites available of ramie stalk, is believed to b ... | 2016 | 26819396 |
| effects of exogenous calcium and spermidine on cadmium stress moderation and metal accumulation in boehmeria nivea (l.) gaudich. | cadmium (cd) is a detrimental metal in the environment and it is easily taken up by plants, thus entering the food chain and posing a severe threat to human health. phytoremediation being low cost, highly stable, and environmentally friendly has been considered as a promising green technology for cd remediation. the addition of exogenous substances to the culture media has been recognized as an efficient strategy to improve plant phytoremediation capability. pot trials were conducted to investig ... | 2016 | 26801927 |
| growth, photosynthesis, and defense mechanism of antimony (sb)-contaminated boehmeria nivea l. | ramie (boehmeria nivea l.) is the oldest cash fiber crop in china and is widely grown in antimony (sb) mining areas. to evaluate the extent of sb resistance and tolerance, the growth, tolerance index (ti), sb content in plant parts and in hoagland solution, bioaccumulation factor (bf), photosynthesis, and physiological changes in sb-contaminated b. nivea (20, 40, 80, and 200 mg l(-1) sb) grown hydroponically were investigated. the sb tolerance and resistance of ramie were clearly revealed by gro ... | 2016 | 26711292 |
| secretome analysis of pleurotus eryngii reveals enzymatic composition for ramie stalk degradation. | pleurotus eryngii (p. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass. in spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible. identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation. in this report, secretomic analysis was used to search for the new or interesting enzymes for ligno ... | 2016 | 26525014 |
| pretreatment by naoh swelling and then hcl regeneration to enhance the acid hydrolysis of cellulose to glucose. | a simple pretreatment method, naoh swelling at a low temperature and then hcl regeneration, was developed for depolymerization of cellulose to glucose. cellulose was transformed into amorphous hydrogel during the treatment; and due to the easy diffusion of h(+) and cl(-) ions into the cellulose hydrogel network as well as the strong ability of cl(-) to disrupt the massive hydrogen bond, the hydrolysis rate was greatly enhanced. the method is effective for α-cellulose, microcrystalline cellulose, ... | 2015 | 26280097 |
| efficient production of glucose by microwave-assisted acid hydrolysis of cellulose hydrogel. | to improve the production of glucose from cellulose, a simple and effective route was developed. this process uses a combination of a step of cellulose dissolution in aqueous naoh/urea solution and then regeneration with water, followed by an acid hydrolysis step under microwave irradiation. the method is effective to obtain glucose from α-cellulose, microcrystalline cellulose, filter paper, ramie fiber and absorbent cotton. increased with the acid concentration the glucose yield from hydrogel h ... | 2015 | 26038330 |
| oncologic long-term results of robot-assisted minimally invasive thoraco-laparoscopic esophagectomy with two-field lymphadenectomy for esophageal cancer. | open transthoracic esophagectomy is the worldwide gold standard in the treatment of resectable esophageal cancer. robot-assisted minimally invasive thoraco-laparoscopic esophagectomy (ramie) for esophageal cancer may be associated with reduced blood loss, shorter intensive care unit (icu) stay, and less cardiopulmonary morbidity; however, long-term oncologic results have not been reported to date. | 2015 | 26023036 |
| boehmeria nivea attenuates the development of dextran sulfate sodium-induced experimental colitis. | we examined the therapeutic effect of an ethanol extract derived from boehmeria nivea (linn.) gaudich in a mouse model of experimental colitis. treatment with 70% ethanol extract derived from b. nivea (ebn) at a dose of 100, 200, or 500 mg/(kg · d) improved colon shortening, body weight, the disease activity index (dai), and histopathological score of dss-induced colitis mice. dss significantly increased the levels of cyclooxygenase-(cox-) 2 in colon tissue relative to that of the untreated cont ... | 2014 | 25045208 |
| variability of cadmium, lead, and zinc tolerance and accumulation among and between germplasms of the fiber crop boehmeria nivea with different root-types. | crop germplasms substantially vary in their tolerance for and accumulation of heavy metals, and assessment of this variability plays a significant role in selecting species to use in phytoremediation projects. here, we examined germplasm-variations in cadmium (cd), lead (pb), and zinc (zn) tolerance and accumulation in ramie (boehmeria nivea), a fiber crop native to china, which has received little attention. in an 8-week greenhouse test, fourteen germplasms of ramie, among and within deep, midd ... | 2015 | 25948381 |
| [determination of cotton content in cotton/ramie blended fabric by nir spectra and variable selection methods]. | rapid detection of textile fiber components is very important for production process of quality control, trading and market surveillance. the objective of this research was to assess cotton content in cotton/ramie blended fabric quickly by near infrared (nir) spectrum technology and variable selection methods. reflectance spectra of samples were acquired by a nirflex n-500 fourier spectroscopy in the range of 4000~10,000 cm(-1), primary election of spectral range and pretreatment analysis were c ... | 2014 | 25881419 |
| diversity of arbuscular mycorrhizal fungi associated with a sb accumulator plant, ramie (boehmeria nivea), in an active sb mining. | arbuscular mycorrhizal fungi (amf) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. however, little information is available about the symbiosis of amf associated with an antimony (sb) accumulator plant under natural conditions. therefore, the objective of this study was to investigate the colonization and molecular diversity of amf associated with the sb accumulator ramie (boehmeria nivea) growing in sb-contaminated soils. four sb mine s ... | 2015 | 25876600 |
| molecular identity of ramie germplasms using simple sequence repeat markers. | dna identity is highly effective and efficient for distinguishing crop varieties regardless of their phenotypic similarities. to establish dna identity in ramie, 21 simple sequence repeat primers were amplified in 108 accessions of domestic and exotic ramie germplasms. sixty polymorphic bands were obtained, with an average of 2.9 bands per locus and 2-8 band types per primer locus (average of 5.19 band types). the simpson's diversity index of the 21 simple sequence repeat loci ranged from 0.158 ... | 2015 | 25867376 |
| start codon targeted (scot) polymorphism reveals genetic diversity in wild and domesticated populations of ramie (boehmeria nivea l. gaudich.), a premium textile fiber producing species. | twenty-four start codon targeted (scot) markers were used to assess genetic diversity and population structure of indigenous, introduced and domesticated ramie (boehmeria nivea l. gaudich.). a total of 155 genotypes from five populations were investigated for scot polymorphism, which produced 136 amplicons with 87.5% polymorphism. polymorphism information content and resolving power of the scot markers were 0.69 and 3.22, respectively. the indian ramie populations exhibited high scot polymorphis ... | 2015 | 25750860 |
| expression profiling of cadmium response genes in ramie (boehmeria nivea l.) root. | ramie (boehmeria nivea), a perennial herb belongs to urticaceae family, is a rapid growth and high biomass crop with highly tolerant and accumulative to heavy metals. however, the gene expression and regulation caused by cadmium (cd) in ramie has not been well studied. in the present study, a gene expression database of ramie root in the absence (control) or presence of 100 μm cd was established. solexa high-throughput sequencing technology showed that 3,654,395 and 3,572,333 tags have been obta ... | 2015 | 25724673 |
| correction: transcriptome sequencing and profiling of expressed genes in phloem and xylem of ramie (boehmeria nivea l. gaud). | [this corrects the article doi: 10.1371/journal.pone.0110623.]. | 2015 | 25679512 |
| [robot-assisted minimally invasive esophagectomy. german version]. | esophagolymphadenectomy is the cornerstone of multimodality treatment for resectable esophageal cancer. the preferred surgical approach is transthoracic, with a two-field lymph node dissection and gastric conduit reconstruction. a minimally invasive approach has been shown to reduce postoperative complications and increase quality of life. robot-assisted minimally invasive esophagectomy (ramie) was developed to facilitate this complex thoracoscopic procedure. ramie has been shown to be safe with ... | 2016 | 27484825 |
| construction and co-expression of polycistronic plasmids encoding bio-degumming-related enzymes to improve the degumming process of ramie fibres. | to research the inherent properties of the co-expression of three types degumming-related enzymes and breed more powerful degumming strains. | 2016 | 27627896 |
| identification of ramie genes in response to pratylenchus coffeae infection challenge by digital gene expression analysis. | root lesion disease, caused by pratylenchus coffeae, seriously impairs the growth and yield of ramie, an important natural fiber crop. the ramie defense mechanism against p. coffeae infection is poorly understood, which hinders efforts to improve resistance via breeding programs. in this study, the transcriptome of the resistant ramie cultivar qingdaye was characterized using illumina sequence technology. about 46.3 million clean pair end (pe) reads were generated and assembled into 40,826 unige ... | 2015 | 26378527 |
| genome-wide transcriptional changes of ramie (boehmeria nivea l. gaud) in response to root-lesion nematode infection. | ramie fiber extracted from stem bark is one of the most important natural fibers. the root-lesion nematode (rln) pratylenchus coffeae is a major ramie pest and causes large fiber yield losses in china annually. the response mechanism of ramie to rln infection is poorly understood. in this study, we identified genes that are potentially involved in the rln-resistance in ramie using illumina pair-end sequencing in two rln-infected plants (inf1 and inf2) and two control plants (co1 and co2). approx ... | 2014 | 25218245 |
| robot-assisted minimally invasive esophagectomy. | esophagolymphadenectomy is the cornerstone of multimodality treatment for resectable esophageal cancer. the preferred surgical approach is transthoracic, with a two-field lymph node dissection and gastric conduit reconstruction. a minimally invasive approach has been shown to reduce postoperative complications and increase quality of life. robot-assisted minimally invasive esophagectomy (ramie) was developed to facilitate this complex thoracoscopic procedure. ramie has been shown to be safe with ... | 2017 | 27470056 |
| [robot-assisted radical esophagectomy]. | robot-assisted esophagectomy for esophageal cancer began in 2003. both of robot-assisted minimally invasive esophagectomy (ramie) and robot-assisted transhiatal esophagectomy (rate) have been applied to date. their reports show that the short term results focused on the safety and oncological aspects are equal to the conventional procedures, though the robot-assisted esophagectomy still does not spread. we have developed the new procedure, a non-transthoracic esophagectomy combining a video-assi ... | 2016 | 27440039 |
| enhanced efficiency of cadmium removal by boehmeria nivea (l.) gaud. in the presence of exogenous citric and oxalic acids. | boehmeria nivea (l.) gaud. is a potential candidate for the remediation of cd contaminated sites. the present investigation aims to explore cd tolerance threshold and to quickly identify the role of exogenous organic acids in cd uptake and abiotic metal stress damage. elevated cd levels (0-10mg/l) resulted in an obvious rise in cd accumulation, ranging from 268.0 to 374.4 in root and 25.2 to 41.2mg/kg dry weight in shoot, respectively. citric acid at 1.5 mmol/l significantly facilitated cd uptak ... | 2014 | 25499499 |
| cloning of expansin genes in ramie (boehmeria nivea l.) based on universal fast walking. | gene cloning is the first step to study the expression profiles and functions of a particular gene; considerable cloning methods have been developed. expansin, thought to involve in the cell-wall modification events, was not cloned in ramie (boehmeria nivea l.), which is one of the most important bast fiber crops with little conducted molecular research, especially on its fiber development. studying the expansin gene family will uncover its possible relationship with ramie fiber development and ... | 2015 | 25481635 |
| transcript profiling reveals auxin and cytokinin signaling pathways and transcription regulation during in vitro organogenesis of ramie (boehmeria nivea l. gaud). | in vitro organogenesis, one of the most common pathways leading to in vitro plant regeneration, is widely used in biotechnology and the fundamental study of plant biology. although previous studies have constructed a complex regulatory network model for arabidopsis in vitro organogenesis, no related study has been reported in ramie. to generate more complete observations of transcriptome content and dynamics during ramie in vitro organogenesis, we constructed a reference transcriptome library an ... | 2014 | 25415356 |
| transcriptome sequencing and profiling of expressed genes in phloem and xylem of ramie (boehmeria nivea l. gaud). | ramie (boehmeria nivea l. gaud) is a highly versatile herbaceous plant which is widely cropped in southern china. the success of this herbaceous plant relies on wide use in modern industry. understanding the profiling of expressed genes in phloem and xylem of ramie is crucial for improving its industrial performance. herein, we uncover the transcriptome profile in phloem and xylem in present study. using illumina paired-end sequencing technology, 57 million high quality reads were generated. de ... | 2014 | 25354139 |
| transcriptome profiling using pyrosequencing shows genes associated with bast fiber development in ramie (boehmeria nivea l.). | ramie (boehmeria nivea l.), popularly known as "china grass", is one of the oldest crops in china and the second most important fiber crop in terms of area sown. ramie fiber, extracted from the plant bast, is important in the textile industry. however, the molecular mechanism of ramie fiber development remains unknown. | 2014 | 25339420 |
| phytochemical profiles and antioxidant activities in six species of ramie leaves. | increased consumption of vegetables or plant food has been associated with decreased risk of developing major chronic diseases, such as cancers, diabetes, cardiovascular diseases, and age-related functional decline. ramie leaves are rich in phenolics and flavonoids, which have been suggested for human health benefits. phenolic contents, flavonoid contents, phenolic compounds, and anti-cancer properties in six species of ramie leaves were analyzed by folin-reagent method, sodium borohydride/chlor ... | 2014 | 25243741 |
| effect of exogenous nitric oxide on antioxidative system and s-nitrosylation in leaves of boehmeria nivea (l.) gaud under cadmium stress. | cadmium (cd)-induced growth inhibition is one of the primary factors limiting phytoremediation effect of boehmeria nivea (l.) gaud in contaminated soil. sodium nitroprusside (snp), a donor of nitric oxide (no), has been evidenced to alleviate cd toxicity in many plants. however, as an important mechanism of no in orchestrating cellular functions, s-nitrosylation is still poorly understood in its relation with cd tolerance of plants. in this study, higher exogenous no levels were found to coincid ... | 2015 | 25242592 |
| intraoperative near-infrared fluorescence imaging as an adjunct to robotic-assisted minimally invasive esophagectomy. | during esophagectomy, identification and preservation of the right gastroepiploic vascular arcade are critical and may be challenging with minimally invasive approaches. we assessed the use of near-infrared fluorescence imaging fluorescence angiography (nifi-fa) during robotic-assisted minimally invasive esophagectomy (ramie) as an aid to visualize the gastric vasculature with mobilization. after intravenous administration of 10 mg of indocyanine green, a robotic platform with near-infrared opti ... | 2015 | 25238427 |
| fast adsorption of cd²⁺ and pb²⁺ by egta dianhydride (egtad) modified ramie fiber. | in this study, the removal of cd(2+) and pb(2+) from aqueous solutions was investigated using a novel chelating material. the first part described the synthesis of ethylene glycol-bis(2-aminoethylether)-n,n,n',n'-tetraacetic acid dianhydride (egtad), mercerization treatment of ramie fiber (mrf), and the mrf was then reacted with egtad to prepare the new material (erf). the obtained material was characterized by weight gain, sem, ftir, and elemental analysis. the results of ftir and elemental ana ... | 2014 | 25181330 |
| [pretreatment of ramie and kenaf stalk for bioethanol production]. | ramie and kenaf were traditional fiber crops in china, but their stalk after decorticating has not been used effectively. the stalk contains a lot of cellulose, and can therefore be used for the production of bioethanol. we studied the effects of different chemical pretreatment on enzymatic digestibility of ramie stalk and kenaf stalk. ramie and kenaf stalks pretreated with alkali were chosen to produce ethanol using quasi-simultaneous saccharification and fermentation (q-ssf) process. the resul ... | 2014 | 25118401 |
| a proteomics sample preparation method for mature, recalcitrant leaves of perennial plants. | sample preparation is key to the success of proteomics studies. in the present study, two sample preparation methods were tested for their suitability on the mature, recalcitrant leaves of six representative perennial plants (grape, plum, pear, peach, orange, and ramie). an improved sample preparation method was obtained: tris and triton x-100 were added together instead of chaps to the lysis buffer, and a 20% tca-water solution and 100% precooled acetone were added after the protein extraction ... | 2014 | 25028960 |
| evaluation of heavy metal and polycyclic aromatic hydrocarbons accumulation in plants from typical industrial sites: potential candidate in phytoremediation for co-contamination. | the heavy metal and polycyclic aromatic hydrocarbons (pahs) contents were evaluated in surface soil and plant samples of 18 wild species collected from 3 typical industrial sites in south central china. the accumulative characteristics of the plant species for both heavy metal and pahs were discussed. the simultaneous accumulation of heavy metal and pahs in plant and soil was observed at all the investigated sites, although disparities in spatial distributions among sites occurred. both plant an ... | 2014 | 24946706 |
| transcriptome comparison reveals the patterns of selection in domesticated and wild ramie (boehmeria nivea l. gaud). | ramie is an old fiber crop, cultivated for thousands of years in china. the cultivar ramie evolved from the wild species qingyezhuma (qyzm, boehmeria nivea var. tenacissima). however, the mechanism of domestication of this old fiber crop is poorly understood. in order to characterize the selective pattern in ramie domestication, orthologous genes between the transcriptomes of domesticated ramie variety zhongzhu 1 (zz1) and wild qyzm were assessed using bidirectional best-hit method and ratio of ... | 2014 | 24934879 |
| robotic-assisted minimally invasive esophagectomy: the ivor lewis approach. | robotic-assisted minimally invasive esophagectomy (ramie) is emerging as a potential alternative approach to standard minimally invasive esophagectomy (mie). however, early reports vary widely in operative approach, method, and reporting of outcomes, including operative complications. a formal, prospective academic program to evaluate ramie was initiated at the authors' institution, with the primary goal of maximizing patient safety during the introduction of new technology into the operating ro ... | 2014 | 24780426 |
| identification of 32 full-length nac transcription factors in ramie (boehmeria nivea l. gaud) and characterization of the expression pattern of these genes. | nam, ataf, and cuc (nac) genes are plant-specific transcription factors (tfs) that play key roles in plant growth, development, and stress tolerance. to date, none of the ramie nac (bnnac) genes had been identified, even though ramie is one of the most important natural fiber crops. in order to mine the bnnac tfs and identify their potential function, the search for bnnac genes against two pools of unigenes de novo assembled from the rna-seq in our two previous studies was performed, and a total ... | 2014 | 24691727 |
| structural basis for unique hierarchical cylindrites induced by ultrahigh shear gradient in single natural fiber reinforced poly(lactic acid) green composites. | a local shear flow field was feasibly generated by pulling the ramie fiber in single fiber reinforced poly(lactic acid) (pla) composites. this was featured by an ultrahigh shear gradient with a maximum shear rate up to 1500 s(-1), a level comparable to that frequently occurring during the practical polymer processing. to distinguish shear-induced self-nucleation and ramie fiber-induced heterogeneous nucleation, the shear history was classified by pulling the fiber for 5 s (pulled sample) and pul ... | 2014 | 24650138 |
| comparative proteome analysis of the response of ramie under n, p and k deficiency. | ramie is an important natural fiber. there has been little research on the molecular mechanisms of ramie related to the absorption, utilization and metabolism of nitrogen (n), phosphorus (p) and potassium (k). one approach to reveal the mechanisms of n, p and k (npk) utilization and metabolism in ramie is comparative proteome analysis. the differentially expressed proteins in the leaves of ramie were analyzed by proteome analysis after 6 days of n- and k-deficient treatments and 3 days of p-defi ... | 2014 | 24573224 |
| agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [boehmeria nivea (l.) gaud]. | in this study, leaf midribs, the elite explants, were used for the first time to develop an efficient regeneration and transformation protocol for ramie [boehmeria nivea (l.) gaud.] via agrobacterium-mediated genetic transformation. sensitivity of leaf midribs regeneration to kanamycin was evaluated, which showed that 40 mg l(-1) was the optimal concentration needed to create the necessary selection pressure. factors affecting the ramie transformation efficiency were evaluated, including leaf ag ... | 2014 | 24488319 |