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[aspects related to the antigenic structure and serological specificity of brucellae phylogenetically related to s- and r-forms and dissociated into r-variants].the antigenic structures and serological properties of brucellae s- and r-forms, phylogenetically differentiated, and dissociated r-variants are studied by means of gel-precipitation, immunoelectrophoresis and fixation of the complement. the brucella suis 1330s, brucella suis 1330r, brucella ovis 02 and brucella abortus 99 are involved in the experiments. both specific and general antigenic structures are established in all strains studied, but only homologous antibodies from the antigen-antibod ...197888791
identification of amino sugars from bacterial lipopolysaccharides by gas chromatography electron impact and chemical ionization mass spectrometry.amino sugars isolated from lipopolysaccharides of brucella suis, brucella abortus and neisseria gonorrhoeae colony types 1 and 4 were identified using gas chromatography electron impact and chemical ionization mass spectrometry. lipopolysaccharides were obtained by aqueous ether or aqueous phenol extraction. isolated lipopolysaccharides were hydrolyzed in 1% acetic acid followed by hydrolysis of the polysaccharide moiety in 2 nhcl for 6 h at 100 degrees c. amino sugars were first isolated by elu ...1978107981
mononucleosis-like picture. brucella suis infection. 1976176619
[adherent cell and t lymphocyte cooperation for the "in vitro" production of brucella-induced interferon by murine spleen cells (author's transl)].although brucella is a good in vivo inducer of interferon, in vitro infection of murine spleen cells by brucella suis has not, to the present time, led to in vitro synthesis. in the present work we show that normal spleen cells can however synthetize interferon in vitro when cultured together with adherent cells obtained from the spleens of syngeneic mice 45 min after in vivo inoculation. induction and synthesis are thus shown to be distinct phenomena. moreover soluble factors are shown to be in ...1978310656
[brucella suis infection]. 1979423632
[outbreak of brucella suis biotype 2 infection in swine]. 1979451518
brucella-induced cholecystitis.acute cholecystitis is a rare complication of systemic brucellosis. this report details the occurrence of acute noncalculous cholecystitis in which brucella suis was cultured from both the blood and the gallbladder. a discussion of the various diagnostic tests and the recommended therapy for brucellosis is included.1979453141
immunologic properties of soluble materials from brucella species.the purpose in this study was to examine the immunogenic properties of various preparations of aqueous ether extracts of brucella suis and brucella canis. the b suis strain 3b and b canis strain rm-6-66 were grown on tryptose agar, and aqueous ether extracts were prepared from the cells. the ether was removed, and the extracts were clarified by centrifugation for 10 hours at 144,000 x g and fractionated by gel chromatography. the b suis endotoxin-containing precipitate, obtained from aqueous eth ...1979464362
brucella suis infection in pregnant cattle.six pregnant, bos taurus cows with stages of gestation ranging from 11 to 33 weeks were each inoculated into the right conjunctival sac with 0.2 ml of a smooth culture of brucella suis type i containing 27 x 10(6) viable organisms. the 6 cows produced 7 calves of which one single calf and one twin calf were stillborn, the cause of which was not determined. br. suis was not isolated from any of the cows or calves using either special media or guinea pig inoculation. no abnormality was found in an ...1979543824
comparative studies on the ultrastructure of l forms obtained from s and r variants of brucella suis 1330.electron-microscopic studies were carried out on s and r variants of brucella suis 1330 and the production of l forms induced with the use of penicillin. no variations were established in the ultrastructure of the initial forms. however, essential differences were observed in the ultrastructure between the latter and the l-transformed brucellae as well as changes that differentiated the ultrastructure of l forms obtained from s variants from that of l forms obtained from r variants. in both case ...1978636707
brucellosis in reindeer, rangifer tarandus l., inoculated experimentally with brucella suis, type 4.an investigation of brucellosis caused by brucella suis, type 4, in reindeer, rangifer tarandus l., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific transmission might occur among confined animals. titres rose to maximum levels within 1 to 2 months following inoculation, decreased during the next 4 months, and persisted at low ...1978647470
[brucella suis infections in the dog]. 1978697724
cell-mediated immune responses in swine from a herd infected with brucella suis.cell-mediated immune (cmi) responses in swine naturally infected with brucella suis biotype 3, swine suckling an infected sow and brucella-noninfected swine were studied by an in vitro lymphocyte transformation procedure. the antigen used was a soluble antigen prepared from killed cells of b suis biotype 3. lymphocytes were prepared from peripheral swine blood by the ficoll-hypaque technique, suspended in rpmi-1640 medium (1.5 x 10(6) lymphocytes/ml), cultured with b suis-soluble antigen or conc ...1978717873
brucellosis in feral swine in florida.serums from 95 feral swine trapped in glades county, florida, were tested for brucella antibodies, using the standard tube, card, rivanol, and complement-fixation tests. fifty (53%) of the animals were seropositive to at least 1 test. the standard tube test detected 98% of the serums positive to any of the 4 procedures. the prevalence of brucella antibodies was significantly lower (p less than 0.0005) in feral swine less than 6 months old than in those greater than or equal to 6 months old. the ...1978738942
[histoimmunofluorescent demonstration of br. suis in necropsy material].use was made of the direct immunofluorescence method for the demonstration of brucella suis bacteria in paraffin and frozen histosections following the respective handling. the method was tested on necropsy material from experimentally infected test animals and spontaneously infected swine embryos. compared with the cultural and serologic methods the histoimmunofluorescence one showed far better diagnostic possibilities. the use of the histoimmunofluorescence method is suggested along with the r ...1976799861
serologic studies of brucella suis and brucella canis.brucella suis strain 3b and brucella canis strain rm-6-66 were grown on tryptose agar, and aqueous ether extracts were prepared from the cells. the aqueous phase was clarified by centrifugation for a total of 10 hours at 144,700 times g and fractionated with gels and with diethylaminoethyl (deae) cellulose. two fractions from b suis and 1 fraction from b canis were reactive in the indirect hemagglutination test. the sensitizing ability was destroyed by pronase, but was unaffected by ribonuclease ...1975803805
[demonstration of brucella antigens in situ in mouse cells and spleen by electron microscopy].the immunoglobulins of rabbit anti-brucella immunsera were conjugated to horse radish peroxydase and used to revell the bacterial antigens during the experimental infection of mice with brucella suis. the technique seems to make possible the identification of bacteria that have undergone morphological modifications after phagocytosis.1975806390
[nitrofurantoin-test for the differentiation of bordetella bronchiseptica (author's transl)].bordetella bronchiseptica is primarily resistant against nitrofurantoin (mic greater than 200 mug/ml), and this feature can be used for the differentiation of the organism from other gram-negative coccobacteria. nitrofurantoin paper disks (300 mug) failed to affect the growth of 150 strains of b. bronchiseptica isolated from different animal hosts, but they produced marked inhibition zones in the cultures of the followingspecies: pasteurella multocida, pasteurella pneumotropica, pasteurella haem ...1975809944
[development of spleenic infection in the mouse following intravenous inoculation of various species of brucella in smooth (s) or rough (r) phases].the bacterial population in the spleen of mice inoculated intravenously by brucella abortus 544 (s), brucella suis 1330 (s), brucella melitensis 115 (r) or brucella canis rm 666(r) is studied kinetically. like the s strains of brucella, the r strains are able to multiply in the mouse spleen. the consequences for the tests of vaccines against natural infections produced by s or r strains of brucella are discussed.1975810263
[isolation of "brucella suis" biotype 5 from a bitch, in madagascar. validity of the species name "brucella canis" (author's transl)].a gram-negative organism isolated from a btich, in madagascar, was examined by bacteriologic, immunologic and metabolic methods, in parallel with cultures representative of the brucella species. the organism fits well into the genus brucella on the basis of its growth, biochemical and antigenic characteristics and was found to have the metabolic pattern on l-asparagine (-), l-arginine (+) and dl-ornithine (+) that identifies and defines the species brucella suis. it is of rough colonial morpholo ...1975811148
evolution and taxonomy in the genus brucella: concepts on the origins of the contemporary species.the flow of thought on evolution of the organisms in the genus brucella was traced through the literature. the finding of brucella suis type 2 in hares provided impetus for much speculation on the changeability of species characteristics. recent reports, largely by soviet investigators, focus on the subjects of the sweep of evolution in this genus through eons of the geologic time scale and also on the possible parentages of the recently discovered species. these papers were translated and perti ...1976816230
evolution and taxonomy in the genus brucella: steroid hormone induction of filterable forms with altered characteristics after reversion.several strains of brucella abortus, brucella suis, and brucella melitensis were exposed to physiologic concentrations of testosterone, progesterone, and diethylstilbestrol by incorporating them into the growth medium. the hormones induced brucella to form cell wall-defective organisms, including filterable forms. the filterable forms from 1 strain of b melitensis had altered characteristics when it reverted to growth as an intact cell. this is the 1st reported instance of induction of filterabl ...1976816232
[localization of the interferon inducer in brucella suis]. 1976817813
[morphological study of 70 cases of brucellosis in rabbits caused by brucella suis].seventy cases of morphologically developed cases or brucellosis in hares are described. twenty-five of the cases were tested by the cultivation methods and the causative agent was identified as brucella suis. in females the authors found 57 cases, in males 13 cases. brucellosis of hares was characterized by a chronical course, by a conspicuous local progression, and by a predominance of pathological changes in the reproductive organs of both sexes. males showed changes mainly on testes. nodal, s ...1976826004
letter: brucella suis in feral pigs. 1976971150
brucellosis: localized pulmonary nodule due to brucella suis. 1976978014
organ-specificity and diagnostic value of cell-mediated immunity against a liver-specific membrane protein: studies in hepatic and non-hepatic diseases.in chronic active hepatitis (cah, n=58) 70% of the hbsag negative and 48% of the hbsag positive cases showed a cmi against human liver specific proteins (hlpi). using hbsag as antigen only 12% of the hbsag negative and 24% of the hbsag positive cases gave a cmi response. on the basis of hbsag and autoantibodies in the serum cah patients could be divided into 4 subgroups. a close correlation between cmi against hlpi, sex, ana and hl-a-8 could be detected. in a follow-up study of patients with acu ...19751083922
further observations on rangiferine brucellosis in alaskan carnivores.antibodies against rangiferine brucellosis, brucella suis type 4, are commonly found in the serum of various domestic and wild alaskian carnivores which feed on caribou, rangifer tarandus granti, arctic alaska. sled dogs from five native villages on the range of the artic caribou herd, but not from two villages on the the range of the porcupine caribou herd, are commonly infected. wolves (canis lupus) and red foxes (vulpes fulva) are less commonly infected. about 90% of the grizzly bears (ursus ...19751113440
[brucella suis infection(author's transl)]. 19751166453
[fertility problems caused by infectious agents pigs in the netherlands(author's transl)].the concept of fertility problems is defined in the introduction and their economic importance in pig breeding are pointed out. the infectious causes of the problem are reviewed. infections with l. tarassovi and with aujezky's virus regularly lead to practically endemic abortions; brucella suis and swine-plague infections do so sporadically. the smedi syndrom is considered. the clinical symptoms, diagnosis, epizootiology, and the therapeutical and prophylactic measures of these infections are di ...19751172635
[a new brucellaphage "weybridge" isolated from brucella suis 1]. 19751217281
brucella suis infection in philadelphia. a survey of hog fever and asymptomatic brucellosis.examination of hospital and public health records revealed 19 cases of brucellosis diagnosed in philadelphia between 1968 and 1972. a serologic survey at philadelphia's largest hog-processing plant, however, indicated infection in 39% of workers. if extrapolated industry-wide, the total would be several hundred in philadelphia. the infection is usually unrecognized or asymptomatic, since men were active in physically demanding jobs with agglutinin titers in excess of 1:5000. overt illness, usual ...19761247015
[brucellic mammitis in sows].in a herd of pigs, 31% presented serological reactions positive or doubtful to brucellic infection; in 15% of the sows the mammary glands were swollen and purulent; the retromammary ganglions were enlarged and hemorrhagic. bacteriological examination of the pus of mammary lesions showed the presence of brucella suis, which confirmed the diagnosis of brucellic mammitis in the sows. the purulent discharge of the mammary lesions, diluted with physiological serum, agglutinated with the antibrucellic ...19761261741
false aneurysm of the abdominal aorta due to brucella suis.a 48-year-old man presented with a fissured false aneurysms of the abdominal aorta due to brucella suis. clinical findings were lumbosciatic pain, fever, and sudation. diagnosis was reached through abdominal computed tomographic (ct) scan and arteriograms. an extremely large false aneurysm, thrombosed and perforated posteriorly, was found in the infrarenal aorta. semiurgent therapy consisted of resection of the aneurysm and prosthetic dacron graft replacement associated with a transposed omental ...19921467188
minimal requirements for growth of brucella suis and other brucella species.minimal nutritional requirements and temperature limits of growth were studied in brucella suis and, comparatively, in a few other brucella species. in a saline basic medium including thiosulphate, ammonium sulphate and glucose with addition of 2 or 4 vitamins (nicotinic acid, thiamin and panthotenic acid, biotin), 24 out of 25 b. suis, 4/6 b. melitensis and 1/6 b. abortus strains were able to grow. some strains, however, needed to be initially induced to grow by other ingredients, co2, other vi ...19911755918
experimental brucella suis biovar 4 infection in a moose.a moose (alces alces gigas) was inoculated with brucella suis biovar 4 to better understand the effects of brucellosis in this species. serum antibody titers increased rapidly and peaked within 21 to 56 days. fever, leukocytosis, recumbency, anorexia and depression were observed starting 42 days post inoculation. brucella suis biovar 4 was isolated from blood, lymph nodes, liver and spleen.19911920668
brucella suis infections in collared peccaries in venezuela.a bacteriologic and serologic study was conducted on two ranches in the states of apure and guarico, venezuela for brucellosis in collared peccaries (tayassu tajacu). one hundred thirty-nine peccaries were necropsied and tissues were cultured. forty-three isolations of brucella suis biovar 1, were made from lymph nodes and spleens of 25 males and 18 females. antibody to brucella sp. was detected in sera from 122 animals by the rapid plate agglutination, standard tube agglutination, 2-mer-captoet ...19911920669
brucella suis s2, brucella melitensis rev. 1 and brucella abortus s19 living vaccines: residual virulence and immunity induced against three brucella species challenge strains in mice.live attenuated brucella suis s2 vaccine was compared to living vaccines b. abortus s19 and b. melitensis rev. 1 in mice. residual virulence was estimated by ability to multiply and persist in spleen and lymph nodes. immunogenicity was estimated by spleen counts of control and vaccinated mice challenged either with the reference b. abortus 544 strain or with virulent b. melitensis h38 and b. suis 1330 strains. s2 vaccine had lower residual virulence; expressed as 50% recovery time, persistence w ...19902123586
antigenic differences between brucella abortus and brucella melitensis recognized by a monoclonal antibody.by means of somatic hybridization of spleen cells from balb/c mice inoculated with brucella abortus and of a line of murine myeloma, a monoclone was obtained producing an antibody which specifically recognizes an epitope of brucella abortus by means of the agglutination reaction. this epitope is not only on the surface of the homologous strains but on all the brucella abortus biotype i, on the brucella melitensis biotype ii and on the brucella suis biotype ii tested. on the contrary it is not pr ...19862423851
brucella suis biovar 3 infection in a kentucky swine herd.sows from a large farrow-to-finish operation in western kentucky had late-term abortions. boars and breeding-age sows were tested serologically for brucellosis, and 83 of 125 were classified as reactors. no brucellae were isolated from the tissues of 6 unbred reactor sows, but brucella suis biovar 3 was recovered from 5 aborted fetuses. epidemiological studies failed to determine the source of the infection.19892488645
brucellosis in an inuit child, probably related to caribou meat consumption.a 9-year-old inuit boy with brucellosis is presented. the most likely source of his infection was contaminated caribou meat. brucella suis is enzootic in canadian caribou herds and this case indicates that natives of the arctic are a risk group for acquiring brucellosis through the ingestion of raw caribou meat.19892569231
an evaluation of three serological tests for antibody to brucella suis in pigs.the rose bengal plate agglutination test (rbt), the complement fixation text (cft) and the tube agglutination test (tat) were applied to serums from 345 feral and 80 domestic pigs sampled at slaughter. at least 2 of the 3 serological tests were applied to each serum. tissues from all pigs were cultured for brucella suis and the degree of culture effort was categorised from 1 to 4 in decreasing order. fifty-eight feral and 35 domestic pigs were culture-positive. a greater proportion of culture-po ...19892712774
selection of a brucella vaccine strain of low residual virulence by chemical mutagenesis.following incubation of the oral vaccine brucella suis strain 2 with diethyl sulphate (des), a mutant designated strain s105 was selected by screening surviving bacteria for reduced virulence for mice. strain 105 also showed low residual virulence for guinea-pigs and, unlike the parent strain, did not initiate abortion in pregnant sheep and goats after parenteral administration. nevertheless, it was as effective as the parent strain in stimulating protective immunity to brucella melitensis when ...19892795638
brucella suis biotype 4: a case of granulomatous nephritis in a barren ground caribou (rangifer tarandus groenlandicus l.) with a review of the distribution of rangiferine brucellosis in canada.severe granulomatous nephritis caused by brucella suis biotype 4 was found in a barren ground caribou (rangifer tarandus groenlandicus) from northwest territories, canada. a review of the distribution of human and animal cases of brucellosis in northern canada indicated that b. suis biotype 4 is distributed widely and is probably enzootic in most canadian caribou herds.19862902236
[two anti-brucella sf311 and s480 monoclonal antibodies with protective activity against brucella pathogenic for mice].two "protective" monoclonal anti-brucella antibodies are described (sf311-igg2a and s480-iga) both of them accelerate the blood clearance of i.v. inoculated brucella suis 1330 and decrease murine splenic infection on day 7.19852938694
[structural rearrangements of the plasmid r68,45 in cells of brucella suis].the mobilizing activity of the plasmid r68,45 in escherichia coli and brucella abortus has been studied. the plasmid r68,45 has been found to lose its ability to mobilize the chromosome in brucella suis cells. the experiments on the conjugational transfer of r68,45 were confirmed by restriction analysis of the plasmid dna. r68,45 has been shown to lose cma in brucella cells via deletion. the molecular mechanisms of deletion process in brucella and other bacteria are discussed.19883050483
[experimental induction of spontaneous brucellosis infection in guinea pigs by contact with guinea pigs subcutaneously infected with brucella suis, biovar 2]. 19883130713
swine brucellosis in indonesia.brucella suis biotype 1 was isolated from 13.1% of the pigs slaughtered in kapuk jakarta, west java and from 15.09% of the pigs slaughtered in surabaya, east java. the prevalence of b. suis by means of the rose bengal plate test, was 22.3% for west java and 14.9% for east java. the rose bengal plate test detected more b. suis infected animals (73% of the infected animals) than did the complement fixation test (41%) and the serum agglutination test (54.5%). the high infection rate is a potential ...19883176296
isolation of brucella suis biotype 1 from a horse. 19883401168
survey of selected diseases in wild swine in texas.tissue, fecal, and serum specimens and swabs of nasal turbinates and tracheas were collected from 100 wild swine (sus scrofa) from 10 populations in texas and, along with 24 additional serum specimens, were evaluated for selected swine diseases. swine positive for pseudorabies were detected in 7 populations. brucella suis biovar 1 was isolated from 4 swine from 2 populations, but positive serologic results may indicate a more widespread distribution of the organism. all populations contained swi ...19863505919
orally administrable brucellosis vaccine: brucella suis strain 2 vaccine.an orally administrable brucellosis vaccine, brucella suis strain 2 vaccine was developed in china. the characteristics and merits of the vaccine are described. it is effective for oral vaccination of sheep, goats, cattle and pigs and has been widely used for prevention of animal brucellosis in china over the past 15 years. about 30-40 million doses of the vaccine are produced every year.19863541425
brucella suis biotype 1 infection in a dog.brucella suis biotype 1 was isolated from the semen of a dog with hindlimb weakness and a large, firm, left epididymis. a semen sample was oligospermic, with many neutrophils, the numbers of which decreased in serial sampling. a card agglutination test for b abortus and a rapid slide agglutination test for b canis were positive. the modified 2-mercaptoethanol slide agglutination test for b canis and the agar gel immunodiffusion test, using b canis cell wall antigen, were negative. at necropsy, c ...19863759635
[the development of morphologic changes and the antibody response in guinea pigs experimentally infected with brucella suis, biotype 2 bacteria].sixty-two guinea pigs were infected vaginally, perorally, intranasally, conjunctivally and subcutaneously with the bacteria b. suis, biotype 2. the inoculum contained 13.8.10(9) of brucellas in 1 ml of physiological solution. subcutaneous infection lasted 151 days, the other types of infection 56 days. all types of infections resulted in brucellotic changes developing in relation to the type and duration of infection. the least marked changes were observed after peroral infection. brucellosis wa ...19853933165
properties of a new phage lytic for brucella suis. 19734128638
target calcifications of the spleen in chronic brucellosis (brucella suis). 19744212382
[rapid biochemical methods for the differentiation of brucella suis and brucella abortus]. 19664224555
purification of interferon induced with brucella suis. physico-chemical and biological properties. 19674233271
[isolation of brucella suis from the european hare (lepus auropaeus) in argentinea]. 19684234596
[production of interferon in tissue cultures and "in vivo" following infection with brucella suis]. 19674300096
[1st case of human brucellosis due to "brucella suis" observed in france]. 19704313064
examination of two bacterial strains designated "brucella suis biotype 5".the morphological, cultural, biochemical, serological and pathogenic properties of two bacterial strains of the group designated ;br. suis biotype 5' were examined. both strains were found to be atypical of the genus brucella in many of these characteristics. no serological relationship to known brucella strains could be detected. on the basis of the evidence obtained the two strains examined were classified as moraxella duplex and the status of ;br. suis biotype 5' questioned.19734515878
antigens of brucella suis. i. effect of physical & chemical agents. 19724633124
[rheumafactors in the course of brucella suis infection]. 19734693415
[isolation of leptospira serotype pomona and brucella suis from swines from the state of santa catarina, brazil (author's transl)]. 19734808063
homologies of deoxyribonucleic acids from brucella ovis, canine abortion organisms, and other brucella species.the bacterium that causes canine abortion has polynucleotide sequences similar, in deoxyribonucleic acid (dna)-dna homology studies, to those of brucella suis and, by inference from previous data, those of b. abortus and b. melitensis as well as b. neotomae. therefore, the organism causing canine abortion appears to be a member of the genus brucella. dna preparations from serratia marcescens, alcaligenes faecalis, and bordetella bronchiseptica, 58, 62, and 66 mole% guanine plus cytosine, respect ...19684882024
[action of bacteriophages on brucella abortus and on brucella suis]. 19654954320
[brucellosis in equins caused by brucella suis]. 19715168558
[sensitivity of sheep to brucella suis in experimental infection]. 19715170436
sites of localization of brucella suis in swine. 19675257299
brucella suis endocarditis. 19705467309
taxonomic position in the genus brucella of the causative agent of canine abortion.the gram-negative organism causing abortion in dogs was examined in parallel with cultures representative of the brucella species and with bordetella bronchiseptica. the organism fits into the genus brucella and most closely resembles b. suis on the basis of its growth characteristics. it is of rough colonial morphology and is agglutinated by antisera prepared against rough brucella. in mouse toxicity tests, no endotoxic activity could be demonstrated. in contrast to most brucella cultures, it d ...19685689122
ulcers of skin due to brucella suis. report of a case. 19685691194
[infection by brucella suis in mexico]. 19655838575
[brucella suis infection in mexico]. 19655890759
[detection and type differentiation of brucella suis]. 19635896674
solitary pulmonary nodule due to brucella suis. report of a case. 19665907981
antigenic structure of brucella suis spheroplasts.baughn, robert e. (university of tennessee, memphis), and bob a. freeman. antigenic structure of brucella suis spheroplasts. j. bacteriol. 92:1298-1303. 1966.-immunoelectrophoresis was used to differentiate between the antigenic mosaics of normal cells of brucella suis and of spheroplasts prepared by treatment with penicillin, glycine, and a combination of these agents. smooth cells possessed at least 13 antigens, 10 of which were precipitated with homologous antiserum. three additional antigens ...19665924266
immunoelectrophoretic analysis of brucella suis. 19665973222
[enzymatic and morphologic modifications in brucella suis treated with interferon]. 19676035147
pathogenesis of three strains of brucella suis in swine. 19676070958
swine diseases transmissible with artificial insemination.the transport of fresh and frozen semen to be used for artificial insemination creates a mode of disease transmission between farms. normally, semen contains a number of nonpathogenic bacterial contaminants; however, excessive bacterial contamination can result in infertile matings. contamination with a known pathogen, eg, brucella suis, could initiate a serious outbreak of disease in a recipient herd. methods to minimize bacterial contamination of semen include sanitary collecting and processin ...19846090372
ultrastructural localization of nadph-oxidase activity in murine peritoneal macrophages during phagocytosis of brucella. correlation with the production of superoxide anions.the localization of the enzyme nadph oxidase in mouse peritoneal macrophages unstimulated or after phagocytosis of brucella suis was investigated by electron microscopy in normal mice and mice immunized against b. suis. the enzyme was clearly visualized on mitochondrial cristae, smooth endoplasmic reticulum, and the plasma membrane; its activity correlated mainly with the state of the endoplasmic reticulum which itself reflected macrophage activation. the enzyme turnover appeared to be accelerat ...19846143443
studies on brucella interferon: chromatographic behaviour and purification.interferon was induced by infecting mice with brucella suis. serum containing interferon activity was analyzed by chromatography on concanavalian a-sepharose and phenyl-sepharose cl-4b columns. antiviral activity was completely retained by the lectin column indicating that all the interferon molecules are glycosylated. the chromatographic behaviour of brucella interferon on phenyl-sepharose cl-4b shows that, like other interferons, brucella displays hydrophobic properties. however, the hydrophob ...19806154449
taxonomic studies on some atypical strains of brucella suis. 19846365239
comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria.the cellular fatty acid compositions of legionella oakridgensis, brucella suis, pseudomonas aeruginosa, and francisella tularensis were compared after base hydrolysis (saponification), acid hydrolysis, and acid methanolysis procedures were used to release the fatty acids. the branched-chain, unsaturated, saturated, and ester-linked hydroxy acids were released as effectively with saponification at 100 degrees c for 30 min as with acid hydrolysis or acid methanolysis at 85 degrees c for 16 h. alth ...19836418758
[brucellosis in rabbits induced by an experimental infection with brucella suis germs].hares were experimentally infected with brucella suis germs, biotype 2, to study the development of morphological changes in the organs. it was demonstrated that conjunctival and intranasal infection caused morphological brucellosis changes in liver. after conjunctival infection affections were observed in 16 days, and in 27 days after intranasal infection. the morphology of changes did not differ from those occurring in spontaneous brucellosis. the results of morphological examination correspon ...19846426134
[morphological changes in brucellosis in guinea pigs due to experimental infection with brucella suis].sixty-four guinea-pigs were infected with the germs of brucella suis. biotype 2 via the vaginal, peroral, intranasal, conjunctival and subcutaneous routes. in the subcutaneous infection the length of the trial was 151 days and in the other methods of infection 56 days. as found, guinea-pigs are highly sensitive and brucellosis lesions were detected in all cases. brucellosis was chronical and spread locally in the guinea-pigs. in the haematogenic spreading of the disease, lesions were constantly ...19846433534
isolation of brucella suis from cattle. 19846439180
genetic factors involved in murine resistance to experimental brucellosis.c57 b1/6 are more resistant than dba2 mice to iv inoculation of brucella suis 1330. this difference does not concern the blood clearance of the injected bacteria or the number of infective colonies in the spleen at very early (less than 24 h) or at late (greater than 2 months) stages but the splenic infection at intermediate stages with maximal differences between days 7 and 14. the "resistance" character is inherited by f1 and backcrosses as a partially dominant character with polygenic control ...19846593265
induction of autoantibodies and circulating immune complexes in mice after injection of brucella fraction "pi" or inoculation with live brucella suis."pi", a vaccinating fraction with b mitogenic and polyclonal activator (pca) activity extracted from brucella melitensis, was i. p. injected into b6 and db mice. in b6 mice, this led to an important but transient increase in the level of anti-ssdna antibodies, the induction of rheumatoid factor (rf) and the appearance of circulating immune complexes (cic). in db mice, on the other hand, the only significant effect consisted of a slight and ephemeral peak of cic in sera from day 7. similarly, whi ...19836602586
binding of bacteria from the genus brucella to human b lymphocytes.in previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. among these bacteria was a strain of brucella melitensis which bound to all human b lymphocytes. to determine whether the binding of b. melitensis to human b lymphocytes was strain, species, or genus characteristic, we tested the binding of b. melitensis, brucella abortus, brucella ovis, brucella suis, brucella canis and brucella neotomae to human normal a ...19816783546
effects of killed brucella abortus strain 45/20 vaccine on reindeer later challenge exposed with brucella suis type 4.six seronegative pregnant reindeer (rangifer tarandus l) were vaccinated with killed brucella abortus strain 45/20 with added adjuvant. these were challenge exposed with b suis type 4 after 90 days; at the same time, 4 seronegative, nonvaccinated, pregnant reindeer (controls) were given the challenge inoculum. humoral antibodies were detected in the vaccinated reindeer by postvaccination day 14. a marked increase in antibody levels also occurred after they were challenge exposed, but did not rea ...19816784617
[pathomorphological changes in brucellosis in hares].the morphology of 645 cases of brucellosis in hares evoked by the germs of brucella suis, biotype 2, is described. brucellosis had the character of chronic granulomatous process with prevailing changes in liver, spleen and in sex organs of hares of both sexes. in the sex organs of sexually immature hares the changes were detected in 2.26% of animals. the granuloma macroscopy was typical and was most discernible in liver. in the organs there prevailed solitary granulomas of the pea size. in teste ...19826812273
the supposed involvement of dogs carrying brucella suis in the spread of swine brucellosis. 19826821294
case report 221. osteomyelitis of the left femur due to brucella suis. 19836844943
bacteriological aspects of a case of brucella suis infection.brucella sinus biotype 1 was isolated from the blood of a 55 year old tongan woman patient in auckland hospital. problems in identification of this organism are described and discussed19816937803
genetic factors involved in murine susceptibility to experimental brucellosis.c57bl/6 are more resistant than dba2 mice to brucella suis 1330. this difference does not concern the blood clearance of the i.v. inoculated bacteria or the number of infective colonies in the spleen at very early stages but the splenic infection at later stages with maximal differences on day +7. the "resistance" character is inherited by f1 and back-crosses as a partially dominant character with polygenic control and a better expression of resistant factor(s) in females. this phenomenon of sex ...19816957160
development of an enzyme-linked immunosorbent assay for detecting antibodies in sera of brucella suis-infected swine.an enzyme-linked immunosorbent assay was developed using a heat-killed brucella suis antigen for detecting antibodies in the sera of swine from which b. suis was isolated. optimal enzyme-linked immunosorbent assay reactions were obtained using heat-killed b. suis antigen at a concentration comparable to mcfarland standard no. 1. statistically significant differences were observed in the enzyme-linked immunosorbent assay results of 40 animals from which b. suis was isolated and the results for 48 ...19807000319
differentiation of brucella suis biotype 4 from other biotypes by firenze phage. 19827059778
acute and chronic hepatic involvement of brucellosis.two patients with brucellosis are presented; one with acute hepatitis and the other with a chronic draining sinus at the right costal margin which originated from an abscess of the liver. brucella abortus infection may be manifest by nonspecific hepatic inflammation or, occasionally, by granulomatous hepatitis. rarely, cirrhosis may result if untreated. chronic hepatic and splenic involvement, which is characterized by tissue calcification, can be seen in brucella suis infection. suppurative com ...19827084621
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