| [the experimental infection of bats with lyssavirus serotypes 1 and 4]. | the results of intramuscular inoculation of 111 bats (myotis daubentoni, myotis brandtii) with three strains of lyssaviruses--yuli, stade, and 1150 are presented. bats were found to have low susceptibility, especially to strains of serotype 4. after infection with serotype 4 viruses, fewer bats died but within shorter periods than after infection with serotype 1. after inoculation with strain 1150, 60% of bats yielded virus, whereas only 9-24% did after inoculation with type 4 strains. the survi ... | 1992 | 1471342 |
| nucleocapsid gene sequence of a north american isolate of viral haemorrhagic septicaemia virus, a fish rhabdovirus. | viral haemorrhagic septicaemia is the most important viral disease of trout in europe. the causative agent, viral haemorrhagic septicaemia virus (vhsv), a member of the lyssavirus genus of the rhabdoviridae family, was formerly believed to be confined to portions of the european continent; however in 1988, vhsv was isolated from adult chinook (oncorhynchus tshawytscha) and coho (o. kisutch) salmon returning to two hatcheries in the northwestern part of the state of washington, u.s.a. initial fea ... | 1992 | 1634868 |
| rabies-specific production of interleukin-2 by peripheral blood lymphocytes from human rabies vaccinees. | cell-mediated immunity induced by rabies vaccination was studied in humans by the determination of specific interleukin-2 (il-2) production in a large number of donors (postexposure immunized patients and pre-exposure immunized laboratory workers). peripheral blood lymphocytes (pbl) from 35 donors were tested for il-2 production after in vitro stimulation by different rabies and rabies-related viruses. il-2 responses were compared to antibody recognition of these different virus serotypes by ser ... | 1991 | 1771968 |
| t and b cell human responses to european bat lyssavirus after post-exposure rabies vaccination. | t and b cell human responses to european bat lyssavirus (ebl1) induced by post-exposure rabies vaccination (pm virus vaccine) were evaluated by measuring plasmatic titres of ebl1-specific neutralizing antibodies; specific ebl1-binding antibodies; and proliferation indices of peripheral blood lymphocytes stimulated in vitro with ebl1. these parameters for vaccination efficacy were compared with those obtained with vaccine-related viruses (cvs and era) and with a non-vaccine-related virus. mokola ... | 1991 | 1864001 |
| serological evidence of infection of dogs and man in nigeria by lyssaviruses (family rhabdoviridae). | a survey was conducted for serum neutralizing antibodies against 3 members of the lyssavirus group (rabies, lagos bat and mokola viruses) in nigerian dogs and humans. of 463 unvaccinated dogs sampled, 142 (30.7%) had antibodies against rabies; 39.2% of the stray dogs were positive. of 241 dogs tested, 17.4% had antibodies against mokola virus and 5.8% against lagos bat virus. 28.6% of human samples tested had antibodies against rabies and, of 158 human samples tested, 12 (7.5%) had antibodies ag ... | 1990 | 2096520 |
| human monoclonal antibodies specific for the rabies virus glycoprotein and n protein. | human monoclonal antibodies to rabies virus were established by epstein-barr virus infection of peripheral blood lymphocytes collected from a rabies-vaccinated donor, and fusion with a heteromyeloma line. two human monoclonal antibodies, hum1 and hum2, both igg2, reacted with the envelope glycoprotein of the rabies virus. the antibody hum1 neutralized rabies virus (lyssavirus serotype 1) and mokola virus (lyssavirus serotype 3), but did not neutralize european bat lyssavirus, suggesting that som ... | 1990 | 2391499 |
| use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. i. the nucleocapsid protein. | twenty-one hybridoma cultures, obtained through the fusion of mouse myeloma cells with splenocytes of balb/c mice immunized with either rabies virus or mokola virus, secreted monoclonal antibodies specific for the nucleocapsid of the inducer virus. they displayed different specificities for the nucleocapsids of rabies and rabies-related viruses and could be classified into eight groups which are likely to correspond to different antigenic determinants on the nucleocapsid. four strains of fixed r ... | 1980 | 6155436 |
| expression of the nucleoprotein of rabies virus in escherichia coli and mapping of antigenic sites. | investigations were performed to delineate the antigenic sites i and iv of rabies virus nucleoprotein (n), the former of which is well conserved among the rabies and rabies-related viruses. the n cdna of the rc-hl strain was inserted into an expression vector pet3a, with which the e. coli bl21(de3) was transformed. upon induction with isopropyl-1-thio-beta-d-galactoside, the transformants produced a protein with a size (56 k-da) almost identical to that of the authentic n protein. the protein al ... | 1995 | 7611878 |
| genetic polymorphism in the rabies virus nucleoprotein gene. | in an attempt to compare intrinsic and extrinsic genetic diversity of the lyssavirus genotypes, 69 rabies virus isolates from various part of the world were partially sequenced and compared to 13 representative isolates of the 6 lyssavirus genotypes. the analysis of their phylogenetic relationships, performed on the complete nucleoprotein (n) coding gene (1350 bases), established that the rabies virus isolates all belonged to genotype 1 and that at least 11 phylogenetic lineages could be identif ... | 1995 | 7778285 |
| vhs confirmed on scottish island. | | 1994 | 7817512 |
| the biology of southern african lyssavirus variants. | | 1994 | 7859494 |
| functional aspects of lyssavirus proteins. | | 1994 | 7859495 |
| beyond pasteur to 2001: future trends in lyssavirus research? | | 1994 | 7859497 |
| reproduction of lyssaviruses: ultrastructural composition of lyssavirus and functional aspects of pathogenesis. | lyssaviruses are considerably adapted to neural tissue, although they can also be replicated in muscle and glandular cells. in neural tissue their reproduction takes place almost exclusively in neurons, and in the course of their dissemination they make use of the structural peculiarities of this highly differentiated cell type. the replication takes place completely in the cytoplasm, although rhabdovirus leader rna enters the nucleus and by blocking host dna and rna synthesis promotes viral syn ... | 1994 | 7859498 |
| the molecular basis for altered pathogenicity of lyssavirus variants. | | 1994 | 7859499 |
| the pathogenesis of rabies and other lyssaviral infections: recent studies. | | 1994 | 7859501 |
| analysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus. | the complete nucleotide sequence of the nucleocapsid (n) gene of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of plant rhabdoviruses, has been determined from cdna clones of both the viral genomic and messenger rnas. the previously identified n gene was adjacent to the 3' leader rna and began at position 91 from the 3' end of the lnyv genome. analysis of the sequence showed that the n mrna contained a 78-nt 5' untranslated region, followed by a 1377-nt open ... | 1994 | 8030210 |
| [experimental lyssavirus infection in chiropters]. | insectivorous bats, pipistrellus pipistrellus in the active stage and hibernation were inoculated with bat unclassified lyssavirus aravan and lyssavirus serotypes 1 and 4. the influence of hibernation on the duration of the incubation period and the distribution of viruses in extraneural tissues was demonstrated. clinical symptoms of the diseases caused by different strains are described. | 1994 | 8160441 |
| genomic organization of lettuce necrotic yellows rhabdovirus. | we have mapped the genome of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of the family rhabdoviridae. we have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded rna genome of lnyv. the lnyv genome appears to contain six genes, the five expected genes coding for the virion proteins, and a sixth gene of unknown function, as for sonchus yellow net virus (synv), a member of the genus nucleorhabdo ... | 1994 | 8178430 |
| mokola virus glycoprotein and chimeric proteins can replace rabies virus glycoprotein in the rescue of infectious defective rabies virus particles. | a reverse genetics approach which allows the generation of infectious defective rabies virus (rv) particles entirely from plasmid-encoded genomes and proteins (k.-k. conzelmann and m. schnell, j. virol. 68:713-719, 1994) was used to investigate the ability of a heterologous lyssavirus glycoprotein (g) and chimeric g constructs to function in the formation of infectious rv-like particles. virions containing a chloramphenicol acetyltransferase (cat) reporter gene (sdi-cat) were generated in cells ... | 1995 | 7853476 |
| lyssavirus in eptesicus serotinus (chiroptera: vespertilionidae). | to determine the prevalence of european bat lyssavirus 1 (ebl1), antibodies plasma samples were obtained from 175 serotine bats (eptesicus serotinus) from four colonies in southern spain between september of 1991 and september 1992. five bats were detected with ebl1 virus in one colony in 1989. the prevalence of antibodies rose to 74% in one of the colonies studied (villarrasa) in the spring of 1992. after a few months the prevalence declined to under 10%. individuals with a high antibody level ... | 1995 | 8592359 |
| further isolation of mokola virus in south africa. | | 1996 | 8650912 |
| [lissaviruses]. | | 1996 | 8669140 |
| the antigen-specific cell-mediated immune response in mice is suppressed by infection with pathogenic lyssaviruses. | responsiveness of t cells (rtc) was studied in balb/c mice intramuscularly infected with various lyssaviruses. after infection by this peripheral route, two types of viruses could be classified according to their effects: 1) pathogenic viruses, including fixed rabies pasteur virus (serogenotype 1) and wild viruses belonging to serogenotype 1 (from a rabid fox in france and from a cow infected by a vampire bat in brazil) or to serogenotype 5 (european bat lyssavirus 1); and 2) non-pathogenic viru ... | 1996 | 8880998 |
| molecular analysis of rabies-related viruses from ethiopia. | from brain samples collected from domestic animals in ethiopia, two rabies-related viruses were isolated. according to their reactivity pattern with anti-nucleocapsid monoclonal antibodies, they were characterized as lagos bat virus (isolate eth-58) and mokola virus (isolate eth-16). this classification was confirmed by neutralization experiments with mokola and lagos bat specific antisera. two potent anti-rabies vaccines were unable to protect mice against the two rabies-related viruses. in ord ... | 1993 | 7777313 |
| monoclonal antibody studies on rabies-related viruses. | rabies and rabies-related viruses are divided into four serotypes, although it has been suggested that the inclusion of european bat lyssaviruses results in six genotypes. sixty-four rabies-related viruses were tested against a panel of 36 anti-nucleocapsid monoclonal antibodies prepared from the immunization of balb/c mice with five prototypic rabies-related viruses. reaction patterns obtained confirmed the original distinction between serotype 1-4 viruses and revealed multiple variants of lago ... | 1993 | 7777312 |
| rabies prophylaxis. | in humans, rabies still is a fatally evolving encephalomyelitis caused by a rhabdovirus of the genus lyssavirus. in general, the disease is contracted through a contact with an infected mammal. taxonomically, different rabies and closely related rabies-like viruses can be distinguished. new molecular identification techniques can be utilized as epidemiological tools to study the geographic distribution and presence in different reservoirs of the viruses. antigenic diversity and new insights in t ... | 1996 | 8950840 |
| encephalitis caused by a lyssavirus in fruit bats in australia. | this report describes the first pathologic and immunohistochemical recognition in australia of a rabies-like disease in a native mammal, a fruit bat, the black flying fox (pteropus alecto). a virus with close serologic and genetic relationships to members of the lyssavirus genus of the family rhabdoviridae was isolated in mice from the tissue homogenates of a sick juvenile animal. | 1996 | 8969249 |
| taskforce investigates fatal bat virus. | | 1996 | 9006852 |
| current knowledge on the australian bat lyssavirus. | | 1997 | 9066976 |
| fatal encephalitis and meningitis at the gold coast hospital, 1980 to 1996. | the recent association of a fatal human case of encephalitis with a newly identified lyssavirus apparently acquired from a native australian bat has highlighted the possibility that previous human infections have passed urecognised. infected bats have been identified on the queensland gold coast where extensive close contact between bats and humans has occurred for many years. in an attempt to identify nervously unrecognised cases of fatal lyssavirus encephalitis, the medical records of the gold ... | 1997 | 9079589 |
| australian bat lyssavirus infection in three fruit bats from north queensland. | we report the case findings of australian bat lyssavirus infection in two black flying foxes (pteropus alecto) and one little red flying fox (pteropus scapulatus) from north queensland between january 1995 and august 1996. although the p. alecto case in january 1995 is the first recognised case of australian bat lyssavirus infection in australia, this was a retrospective diagnosis made after identification of the index case at ballina in may 1996. eight persons had exposure to the three bats. se ... | 1997 | 9145563 |
| [isolation and identification of lyssavirus strains from an area of slovakia where oral antirabies vaccine was administered]. | the study was aimed at isolation and subsequent identification of strains of rabies virus by means of monoclonal antibodies from foxes killed in the vaccination zone within the complex preliminary monitoring of oral antirabies vaccination. the results obtained indicate that the vaccines for oral antirabies vaccination used in slovakia did not contain any vaccination strain pathogenic to the extremely sensitive target species-the fox (vulpes vulpes). | 1997 | 9148571 |
| australian bat lyssavirus. | | 1997 | 9196832 |
| zoonoses control. lyssavirus infection in 3 fruit bats, australia. | | 1997 | 9220702 |
| the complete mokola virus genome sequence: structure of the rna-dependent rna polymerase. | the genome sequence of the rabies-related virus mokola virus (genus lyssavirus) has been completed by sequencing the l gene, which consists of 6384 nucleotides encoding a 2127 amino acid polymerase. alignment of the mokola virus l protein with other polymerases from the virus order mononegavirales defined three domains: a divergent nh2-terminal domain, a highly conserved central domain carrying most of the functional motifs and a cooh-terminal domain with alternating conserved and divergent regi ... | 1997 | 9225031 |
| evolution of european bat lyssaviruses. | forty-seven european bat lyssaviruses (ebl) and two african insectivorous bat lyssaviruses (duvenhage viruses) were selected for a comparison to be made of their evolutionary relationships. studies were based on direct sequencing of the pcr-amplified products of the 400 nucleotides coding for the amino terminus of the nucleoprotein. phylogenetic relationships were analysed after bootstrap resampling using the maximum parsimony and the neighbour-joining methods. analyses of both the nucleotide an ... | 1997 | 9292021 |
| taxonomy and evolutionary studies on lyssaviruses with special reference to africa. | currently, the lyssavirus genus is composed of four serotypes and the ungrouped european bat lyssaviruses (ebl). using previously described pcr amplification and direct sequencing methods, the n genes of 70 representative lyssaviruses collected from 40 different countries were examined. from the results, a phylogenetic tree with six branches (genotypes) was constructed: genotypes 1-4 correlated with the classification of serotypes 1-4 and in addition ebl 1 and ebl 2 were identified as two furthe ... | 1993 | 7777311 |
| emerging infections in australia. | over the last 10 years, novel infectious agents including equine morbillivirus, lyssavirus, barmah forest virus, rickettsia honei and two as-yet-unnamed bunyaviruses have been identified as causes of human disease in australia. previously described agents, such as japanese b encephalitis virus, dengue virus, ross river virus, orientia tsutsugamushi, rickettsia australis, burkholderia pseudomallei, mycobacterium ulcerans and trichinella pseudospiralis, have increased their geographical distributi ... | 1997 | 9494666 |
| rapid detection of rabies and rabies-related viruses by rt-pcr and enzyme-linked immunosorbent assay. | a rapid detection method for the six established genotypes of rabies and rabies-related viral rna using rt-pcr-elisa is described. the detection of digoxigenin-labelled amplified products is performed by solution hybridization to two specific, biotin-labelled, capture probes, which are complementary to the inner region of the amplification products. the capture probe and amplified product hybrid are then immobilised on a streptavidin-coated microtitre plate, bound products are detected by an ant ... | 1997 | 9504752 |
| emerging viruses. | an emerging virus is a term applied to a newly discovered virus, one that is increasing in incidence or with the potential to increase in incidence. many viruses fit into this definition. hiv is the clearest example of a previously unknown virus that has now produced one of the largest pandemics in history. recent advances have occurred in the identification and understanding of new hantaviruses in the americas, causing an acute respiratory disease. the possible causal role of human herpesvirus ... | 1998 | 9529635 |
| the rabies virus genome: an overview. | the replication strategy, genome organization and extent of variation within the genome of the genus lyssavirus is briefly reviewed. strategies used in the approach to genome studies are discussed. | 1993 | 7777310 |
| lyssaviral infection and lead poisoning in black flying foxes from queensland. | pteropid lyssaviral infection, lead poisoning, and the difficulties in diagnosing pteropid lyssaviral infection using histopathological examination of tissues are described in wild black flying foxes (pteropus alecto) from northern queensland (australia). an adult female p. alecto showed aggression before death in january 1995. lead poisoning was diagnosed due to the presence of intranuclear lead inclusion bodies in renal proximal convoluted tubular epithelium and high concentrations of lead in ... | 1998 | 9577784 |
| dna-based immunization for exploring the enlargement of immunological cross-reactivity against the lyssaviruses. | dna-based immunization was used for studying the cross-reactivity of lyssavirus neutralizing antibodies and for exploring the induction of a wider range of protection against lyssaviruses. in order to immunize mice with homogeneous and chimeric genes of glycoproteins (g) from two divergent lyssaviruses, we used for the first time a new plasmid (pci-neo) known to be a highly efficient vector for in vitro expression. the homogeneous plasmids pgpv and pgmok encoded the pasteur virus (pv: genotype 1 ... | 1998 | 9607065 |
| the glycoprotein g of rhabdoviruses. | rhabdoviruses show an rna-containing helically-wound nucleocapsid either enclosed by or enclosing a membrane m protein, surrounded by a lipid bilayer through which dynamic protein trimers made up of non-covalently associated monomers of glycoprotein g (g) project outside. mature monomeric rhabdoviral g has more than 500 amino acids, 2-6 potential glycosylation sites, 12-16 highly conserved cysteine residues, 2-3 stretches of a-d hydrophobic heptad-repeats, a removed amino terminal hydrophobic si ... | 1995 | 7605197 |
| bat lyssavirus prophylaxis in an immunocompromised patient. | | 1998 | 9734519 |
| molecular epidemiology of lyssaviruses: focus on the glycoprotein and pseudogenes. | | 1993 | 7539907 |
| linear and conformation-dependent antigenic sites on the nucleoprotein of rabies virus. | a set of 29 monoclonal antibodies (mabs) specific for the rabies virus nucleoprotein (n protein) was prepared and used to analyze the topography of antigenic sites. at least four partially overlapping antigenic sites were delineated on the n protein of rabies virus by competitive binding assays. indirect immunofluorescent antibody tests using mabs with a series of rabies and rabies-related viruses showed that epitopes shared by various fixed and street strains of rabies virus were mainly localiz ... | 1994 | 7526134 |
| australian lyssavirus infection. | | 1998 | 9834577 |
| frequency analysis of cytolytic t lymphocyte precursors (ctl-p) generated in vivo during lethal rabies infection of mice. ii. rabies virus genus specificity of ctl-p. | cytolytic t lymphocyte precursors (ctl-p) were sensitized in vivo by intraplantar infection of c57bl/6 mice with a lethal dose of rabies virus, strain era (era). as a result of sensitization ctl-p matured to interleukin-receptive ctl-p (il-ctl-p) that could be expanded in vitro to thy-1+, lyt-2+ ctl clones in the presence of il without subjection to antigen-driven selection. after infection with era, il-ctl-p-derived ctl lysed fibroblasts infected with rabies virus but not those infected with an ... | 1984 | 6094202 |
| use of anti-glycoprotein monoclonal antibodies to characterize rabies virus in formalin-fixed tissues. | seventy anti-rabies virus monoclonal antibodies (mabs) were tested for reactivity with rabies and rabies-related viruses in formalin-fixed (ff) tissues. forty-three of the mabs were directed against the glycoprotein and 27 were directed against the nucleocapsid as determined by enzyme immunoassays and neutralization tests. twenty of the anti-glycoprotein mabs and one of the anti-nucleocapsid mabs reacted with the rabies challenge virus strain (cvs) in ff tissue. these 21 mabs were screened again ... | 1999 | 10029326 |
| [rabies]. | | 1999 | 10088358 |
| mokola virus infection: description of recent south african cases and a review of the virus epidemiology. | five cases of mokola virus, a lyssavirus related to rabies, are described. the cases occurred in cats from the east london, pinetown and pietermaritzburg areas of south africa from february 1996 to february 1998. each of the cats was suspected of being rabid and their brains were submitted for laboratory confirmation. four of the cases were positive, but with atypical fluorescence, and 1 was negative. mokola virus infection was identified by anti-lyssavirus nucleocapsid monoclonal antibody typin ... | 1998 | 10192092 |
| immunological relationships between rabies virus and rabies-related viruses studied with monoclonal antibodies to mokola virus. | specific monoclonal antibodies (mab) were prepared against a rabies-related lyssavirus, mokola virus. a strain isolated in the central african republic, mok-3, was used as immunogen. after 3 fusions more than 90 hybridoma cultures secreting mab were identified. according to their different patterns of reactivity against rabies and rabies-related viruses, 61 ascites fluids were obtained. the antibody class was igm for 2 of them and igg for 59. they were specific for one of the 4 major viral prote ... | 1988 | 3207504 |
| australian bat lyssavirus infection in a captive juvenile black flying fox. | the newly emerging australian bat lyssavirus causes rabieslike disease in bats and humans. a captive juvenile black flying fox exhibited progressive neurologic signs, including sudden aggression, vocalization, dysphagia, and paresis over 9 days and then died. at necropsy, lyssavirus infection was diagnosed by fluorescent antibody test, immunoperoxidase staining, polymerase chain reaction, and virus isolation. eight human contacts received postexposure vaccination. | 1999 | 10341182 |
| deduced structural model for animal rhabdovirus glycoproteins. | the g protein sequences of fourteen animal rhabdoviruses, representing all four recognized genera (vesiculovirus, lyssavirus, ephemerovirus and novirhabdovirus) and the ungrouped sigma virus, were aligned using clustal w and adjusted to account for obvious sequence similarities not detected by the algorithm. analysis of the alignment indicated remarkable preservation of g protein structural features including cysteine residues, antigenic sites and significant elements of secondary structure (alp ... | 1999 | 10355768 |
| a retrospective search for lyssavirus in humans in the northern territory. | following the 1996 discovery of a rabies-like lyssavirus in australian flying foxes, it was unclear whether this was a new epizootic or an unrecognised, previously existing disease. | 1999 | 10388177 |
| complete cloning and molecular organization of a rabies-related virus, mokola virus. | mokola virus is a rabies-related virus responsible for both animal and human encephalitis cases in africa. we report here the construction of a genomic library containing overlapping cdna clones encompassing the entire genome. five overlapping clones were sufficient to cover the genome (about 12 kb in size). mokola virus was shown to share the same genomic organization as the rabies virus genome and also identical transcription signals. cdna probes characterized six different transcripts by nort ... | 1989 | 2769229 |
| newly discovered viruses of flying foxes. | flying foxes have been the focus of research into three newly described viruses from the order mononegavirales, namely hendra virus (hev), menangle virus and australian bat lyssavirus (abl). early investigations indicate that flying foxes are the reservoir host for these viruses. in 1994, two outbreaks of a new zoonotic disease affecting horses and humans occurred in queensland. the virus which was found to be responsible was called equine morbillivirus (emv) and has since been renamed hev. inve ... | 1999 | 10501164 |
| immunohistochemistry in the identification of a number of new diseases in australia. | immunohistochemistry plays an important part in the diagnosis of some viral diseases. demonstration of viral antigen in a lesion is an important contribution to diagnosis, either at the time of investigation or retrospectively. at the csiro australian animal health laboratory, the most frequent use of immunohistochemistry has been in the diagnosis of the important avian diseases, highly pathogenic avian influenza and newcastle disease. the technology took key roles in the diagnoses of hendra vir ... | 1999 | 10501165 |
| rabies-related yuli virus; identification with a panel of monoclonal antibodies. | yuli virus was isolated by intracerebral (i.c.) inoculation of suckling mice with a 10% brain suspension from 11-year-old patient who died under signs of atypical hydrophobia after a bat bite into lower lip. identification with a panel of monoclonal antibodies (moab) to nucleocapsid protein (np) confirmed that yuli virus belongs to lyssavirus genus, as an antigenic variant of the european duvenhage virus. | 1989 | 2576595 |
| a reduced panel of anti-nucleocapsid monoclonal antibodies for bat rabies virus identification in europe. | a reduced panel of 4 anti-nucleocapsid monoclonal antibodies (mab) was set up to distinguish viruses of terrestrial mammal origin from viruses of bat origin in europe. four additional mab were necessary to identify each one of the four serotypes of lyssavirus. these 8 mab were selected out of 25 mab secreted by hybridomas obtained from mice immunized with either serotype 1 lyssavirus (rabies virus pv4) or serotype 3 lyssavirus (mokola). they were screened with 32 viruses representative of the fo ... | 1990 | 2277871 |
| histopathology and immunohistochemistry of bats infected by australian bat lyssavirus. | to describe the lesions and distribution of viral antigens in bats infected by australian bat lyssavirus. | 1999 | 10561795 |
| australian bat lyssavirus--a newly emerged zoonosis? | | 1997 | 9088509 |
| assessment of template quality by the incorporation of an internal control into a rt-pcr for the detection of rabies and rabies-related viruses. | a method is described to assess rna template quality by the incorporation of a ribosomal rna (rrna) internal (in tube) control into a standard rabies and rabies-related virus specific rt-pcr. specific virus and rrna templates were co-amplified in a duplex reaction from rna extracts derived from 60 isolates representing all six of the established lyssavirus genotypes. to ensure a wide species applicability of this technique we demonstrated that the rrna assay was capable of functioning using the ... | 2000 | 10680960 |
| veterinary surgeon's guide to australian bat lyssavirus. | veterinary surgeons in australia must be aware of the emerging viral diseases and their potential effects on public health generally and, more specifically, on the veterinary profession. australian bat lyssavirus was identified in 1996 and causes rabies-like disease in bats and humans. two humans from queensland have died of australian bat lyssavirus encephalitis. surveillance has shown that all australian bats must be considered carriers of this new virus, therefore protective apparel should be ... | 1999 | 10685161 |
| from rabies to rabies-related viruses. | antigenic differences between rabies virus strains characterized with monoclonal antibodies presently define at least four serotypes within the lyssavirus genus of the rhabdoviridae family: classical rabies virus strains (serotype 1), lagos bat virus (serotype 2), mokola virus (serotype 3) and duvenhage virus (serotype 4). the wide distribution of rabies-related virus strains (serotypes 2, 3 and 4) and above all, the weak protection conferred by rabies vaccines against some of them (principally ... | 1990 | 2205967 |
| potential exposure to australian bat lyssavirus, queensland, 1996-1999. | two human deaths caused by australian bat lyssavirus (abl) infection have been reported since 1996. information was obtained from 205 persons (mostly adults from south brisbane and the south coast of queensland), who reported potential abl exposure to the brisbane southside public health unit from november 1,1996, to january 31, 1999. volunteer animal handlers accounted for 39% of potential exposures, their family members for 12%, professional animal handlers for 14%, community members who inten ... | 2000 | 10827115 |
| molecular methods to distinguish between classical rabies and the rabies-related european bat lyssaviruses. | a rapid and sensitive reverse transcriptase-polymerase chain reaction (rt-pcr) assay for the detection of classical rabies virus (genotype 1) and the rabies related european bat lyssaviruses (ebls) (genotypes 5 and 6) was developed. when combined with specific oligonucleotide probes and a pcr-enzyme linked immunosorbent assay (pcr-elisa), genotype 5 and 6 viruses can be distinguished from each other and from genotype 1 viruses. ninety-two isolates from the six established genotypes of rabies and ... | 2000 | 10856759 |
| detection of rabies virus antibodies in fruit bats (eidolon helvum) from nigeria. | fruit bats (eidolon helvum) were collected from three different localities in western nigeria: oti, ibadan and idanre. fifty serum samples were analyzed using a modified rapid fluorescent focus inhibition technique against rabies, mokola, lagos bat and duvenhage viruses. twenty-five brain samples were screened for rabies and related lyssavirus antigens by direct fluorescent antibody microscopy and by an indirect fluorescent antibody technique with rabies anti-nucleocapsid monoclonal antibodies. ... | 1990 | 2187101 |
| australian bat lyssavirus: the public health response to an emerging infection. | | 2000 | 10914099 |
| australian bat lyssavirus infection: a second human case, with a long incubation period. | in december 1998, a 37-year-old queensland woman died from a rabies-like illness, 27 months after being bitten by a flying fox (fruit bat). molecular techniques enabled diagnosis of infection with australian bat lyssavirus (abl), the second human case to be recognised and the first to be acquired from a flying fox. it must be assumed that any bat in australia could transmit abl; anyone bitten or scratched by a bat should immediately wash the wounds thoroughly with soap and water and promptly see ... | 2000 | 10914106 |
| cytoplasmic dynein lc8 interacts with lyssavirus phosphoprotein. | using a yeast two-hybrid human brain cdna library screen, the cytoplasmic dynein light chain (lc8), a 10-kda protein, was found to interact strongly with the phosphoprotein (p) of two lyssaviruses: rabies virus (genotype 1) and mokola virus (genotype 3). the high degree of sequence divergence between these p proteins (only 46% amino acid identity) favors the hypothesis that this interaction is a common property shared by all lyssaviruses. the p protein-dynein lc8 interaction was confirmed by col ... | 2000 | 11024152 |
| [genetic diversity of lyssavirus]. | rabies is a zoonosis of which the archaeological agents belong to the lyssavirus species. seven genotypes are known to exist. the isolates are grouped according to a) their geographical origin, b) historical context, c) type of vector. in parts of the world where several epidemic cycles exist, practical tools have been developed in order to distinguish between them more easily. vaccine strains--all of which have been elaborated from genotype 1--offer little or no protection against the other gen ... | 2000 | 11030054 |
| characterisation of a recently isolated lyssavirus in frugivorous zoo bats. | in july 1997 a lyssavirus was isolated in denmark from a colony of egyptian flying foxes (rousettus aegyptiacus) originating from a dutch zoo. sequencing of a 400 nucleotides coding region of the nucleoprotein and of a major part of the g-protein ectodomain encoding region of the newly isolated virus, revealed a very high similarity with european bat lyssavirus subtype 1a (ebl-1a). for characterisation of the recently isolated lyssavirus in frugivorous zoo bats, 16 frugivorous bats (rousettus ae ... | 2000 | 11043951 |
| first isolation of a rabies-related virus from a daubenton's bat in the united kingdom. | on may 30, 1996, a sick daubenton's bat (myotis daubentonii) was recovered from the cellar of a public house in newhaven, east sussex. its condition deteriorated rapidly, and it was euthanased and examined. positive results, establishing the presence of a rabies or rabies-related virus in its brain, were obtained from the fluorescent antibody test, the rabies tissue culture isolation test, and a hemi-nested reverse-transcription pcr. the complete sequence of the nucleoprotein gene was determined ... | 2000 | 11073000 |
| rabies virus cross-reactive murine t cell clones: analysis of helper and delayed-type hypersensitivity function. | three t cell clones derived from rabies virus-immunized balb/c mice were analysed for specificity and function. the clones proved to be broadly cross-reactive by responding to different rabies virus isolates (pm, era, cvs, hep) and other representatives of the genus lyssavirus, like the duvenhage-6 (duv6) and mokola (mok) viruses. the clones detected three different epitopes: an epitope expressed on the matrix protein (m) shared by pm, hep, mok and duv6 viruses (clone aa8), an epitope expressed ... | 1990 | 1967528 |
| an l (polymerase)-deficient rabies virus defective interfering particle rna is replicated and transcribed by heterologous helper virus l proteins. | a rabies virus-derived defective interfering particle (di) was isolated and characterized. the di genome contained an internal deletion of 6.4 kb spanning the 3' moiety of the pseudogene region (psi) and most of the l gene. di-specific monocistronic n, ns, and m mrnas as well as a g/l fusion mrna were transcribed in cells coinfected with di and helper virus. in addition, polycistronic di rnas and standard virus rnas with internal a stretches and intergenic regions were found. superinfection expe ... | 1991 | 1887588 |
| antigenic and genetic divergence of rabies viruses from bat species indigenous to canada. | antigenic characterisation of over 350 chiropteran rabies viruses of the americas, especially from species reported rabid in canada, distinguished 13 viral types. in close accord with this classification, nucleotide sequencing of representative isolates, at both the n and g loci, identified four principal phylogenetic groups (i-iv), sub-groups of which circulated in particular bat species. amongst the north american bat viruses, there was a notable division between group i specimens associated w ... | 2001 | 11226582 |
| evidence of two lyssavirus phylogroups with distinct pathogenicity and immunogenicity. | the genetic diversity of representative members of the lyssavirus genus (rabies and rabies-related viruses) was evaluated using the gene encoding the transmembrane glycoprotein involved in the virus-host interaction, immunogenicity, and pathogenicity. phylogenetic analysis distinguished seven genotypes, which could be divided into two major phylogroups having the highest bootstrap values. phylogroup i comprises the worldwide genotype 1 (classic rabies virus), the european bat lyssavirus (ebl) ge ... | 2001 | 11238853 |
| dna-based immunization against lyssaviruses. | rabies is a fatal encephalomyelitis. most cases occur in developing countries and are transmitted by dogs. because of their high cost, cell culture vaccines have not totally replaced the unsafe brain-derived vaccines which are still used in many developing countries. moreover, there will be a need for vaccines against rabies-related viruses against which classical vaccines are not always effective. dna vaccines would, therefore, be a valuable alternative for the production of cheaper rabies vacc ... | 2000 | 11251386 |
| experimental utility of rabies virus-neutralizing human monoclonal antibodies in post-exposure prophylaxis. | rabies immune globulin (rig) is essential for post-exposure prophylaxis but is expensive and not widely available. rabies virus-neutralizing human monoclonal antibodies (mabs) were evaluated in vitro and in a syrian hamster model as a potential future alternative. seven mabs neutralized representative rabies virus variants. however, a european bat lyssavirus was not neutralized by either mabs or rig. moreover, duvenhage virus was neutralized by rig, but not by mabs, and lagos bat and mokola viru ... | 2001 | 11427255 |
| production and neurotropism of lentivirus vectors pseudotyped with lyssavirus envelope glycoproteins. | we investigated the production efficiency and the gene transfer capacity in the central nervous system of hiv-1-based vectors pseudotyped with either the g protein of the mokola lyssaviruses (mk-g), a neurotropic virus causing rabies disease, or the vesiculo-stomatitis g protein (vsv-g). both envelopes induced syncitia in cell cultures. they were incorporated into vector particles and mature virions were observed by electron microscopy. vector production was two- to sixfold more efficient with v ... | 2001 | 11482987 |
| host switching in lyssavirus history from the chiroptera to the carnivora orders. | lyssaviruses are unsegmented rna viruses causing rabies. their vectors belong to the carnivora and chiroptera orders. we studied 36 carnivoran and 17 chiropteran lyssaviruses representing the main genotypes and variants. we compared their genes encoding the surface glycoprotein, which is responsible for receptor recognition and membrane fusion. the glycoprotein is the main protecting antigen and bears virulence determinants. point mutation is the main force in lyssavirus evolution, as sawyer's t ... | 2001 | 11483755 |
| emerging viral diseases of southeast asia and the western pacific. | over the past 6 years, a number of zoonotic and vectorborne viral diseases have emerged in southeast asia and the western pacific. vectorborne disease agents discussed in this article include japanese encephalitis, barmah forest, ross river, and chikungunya viruses. however, most emerging viruses have been zoonotic, with fruit bats, including flying fox species as the probable wildlife hosts, and these will be discussed as well. the first of these disease agents to emerge was hendra virus, forme ... | 2001 | 11485641 |
| a modified rapid enzyme immunoassay for the detection of rabies and rabies-related viruses: rreid-lyssa. | this paper presents a modification of the previously described rapid rabies enzyme immuno-diagnosis test (rreid) by using biotinylated antibodies, streptavidin conjugate and a mixture of monospecific polyclonal antibodies against several lyssaviruses. in the modified technique (rreid-lyssa), microplates were sensitized with a mixture of purified antibodies against ribonucleoprotein (rnp) from pasteur virus (lyssavirus serotype 1), european bat lyssavirus (ebl, unclassified) and mokola virus (lys ... | 1992 | 1610558 |
| functional interaction map of lyssavirus phosphoprotein: identification of the minimal transcription domains. | lyssaviruses, the causative agents of rabies encephalitis, are distributed in seven genotypes. the phylogenetically distant rabies virus (pv strain, genotype 1) and mokola virus (genotype 3) were used to develop a strategy to identify functional homologous interactive domains from two proteins (p and n) which participate in the viral ribonucleoprotein (rnp) transcription-replication complex. this strategy combined two-hybrid and green fluorescent protein-reverse two-hybrid assays in saccharomyce ... | 2001 | 11559793 |
| screening of active lyssavirus infection in wild bat populations by viral rna detection on oropharyngeal swabs. | brain analysis cannot be used for the investigation of active lyssavirus infection in healthy bats because most bat species are protected by conservation directives. consequently, serology remains the only tool for performing virological studies on natural bat populations; however, the presence of antibodies merely reflects past exposure to the virus and is not a valid marker of active infection. this work describes a new nested reverse transcription (rt)-pcr technique specifically designed for ... | 2001 | 11574590 |
| antigenic and molecular characterization of bat rabies virus in europe. | the predominant role of eptesicus serotinus in the epizootic of bat rabies in europe was further outlined by the first isolation of the rabies virus from this species in france. the distribution of the virus was studied in naturally infected e. serotinus bats at the time of death and suggested that the papillae of the tongue and the respiratory mucosa may play a role in virus production and excretion. the analysis of 501 french rabies virus isolates from various animal species by antinucleocapsi ... | 1992 | 1401009 |
| [a lyssavirus with an unusual antigenic structure isolated from a bat in southern kyrgyzstan]. | examination of 191 specimens of chiroptera in osh province of kyrgyzstan yielded 1 strain of lyssavirus from myotis blythi, the isolate not belonging to serotype 1. the virus was designated aravan by the area of its isolation. its antigenic structure was studied using antinucleocapsid monoclonal antibodies of the wistar institute (philadelphia, isa) and central veterinary laboratory of great britain (waybridge, great britain). the paper presents its antigenic profile, brief characteristics of si ... | 1992 | 1290226 |
| interaction of lyssaviruses with the low-affinity nerve-growth factor receptor p75ntr. | the low-affinity nerve-growth factor receptor p75ntr interacts in vitro with the rabies virus (rv) glycoprotein and serves as a receptor for rv. the lyssavirus genus comprises seven genotypes (gts) of rabies and rabies-related viruses. the ability of p75ntr to interact with the glycoprotein of representative lyssaviruses from each gt was investigated. this investigation was based on a specific binding assay between bsr cells infected with a lyssavirus and spodoptera frugiperda (sf21) cells expre ... | 2001 | 11714960 |
| a novel expression cassette of lyssavirus shows that the distantly related mokola virus can rescue a defective rabies virus genome. | by comparing three expression vectors for the rabies virus (rv) minigenome, we show that the characteristic of the rv rna is important for efficient rescue despite its not being crucial for replication. moreover, we show that the coexpression of the viral proteins from helper rv and mokola virus could rescue the rv minigenome while rv-related european bat lyssavirus 1 could not, suggesting that the signals controlling transcription and replication are conserved in the distantly related rv and mo ... | 2002 | 11799201 |
| emerging viral infections in australia. | emerging viruses include known viruses that have increased in incidence or geographic range (such as enteroviruses and japanese encephalitis virus), new viruses associated with known diseases (australian bat lyssavirus) and new viruses associated with previously unrecognized diseases (hendra and nipah viruses). some may have a predilection for children (japanese encephalitis, influenza viruses and enterovirus 71) and vigilance is essential to ensure early recognition of these agents. | 2002 | 11869391 |
| genetic constraints and the adaptive evolution of rabies virus in nature. | we used a molecular evolutionary approach to investigate the species adaptation of rabies virus in nature. a maximum likelihood analysis of selection pressures revealed that the nucleoprotein (n) and glycoprotein (g) genes of natural viral isolates were highly constrained, especially at nonsynonymous sites, in contrast to the higher rates of nonsynonymous evolution observed in viruses subject to laboratory passage. positive selection was only found at a single amino acid site--position 183 in th ... | 2002 | 11878928 |
| presence of european bat lyssavirus rnas in apparently healthy rousettus aegyptiacus bats. | apparently healthy rousettus aegyptiacus bats were randomly chosen from a dutch colony naturally infected with european bat lyssavirus subgenotype 1a (ebl1a). these bats were euthanised three months after the first evidence of an ebl1a infection in the colony. ebl1a genomic and antigenomic rnas of the nucleoprotein gene were detected by nested reverse transcriptase pcr in 75% of the examined rousettus aegyptiacus bats. the ebl1a rnas of the nucleoprotein gene were detected mainly in brain tissue ... | 2002 | 11892688 |
| serologic evidence of lyssavirus infections among bats, the philippines. | active surveillance for lyssaviruses was conducted among populations of bats in the philippines. the presence of past or current lyssavirus infection was determined by use of direct fluorescent antibody assays on bat brains and virus neutralization assays on bat sera. although no bats were found to have active infection with a lyssavirus, 22 had evidence of neutralizing antibody against the australian bat lyssavirus (ablv). seropositivity was statistically associated with one species of bat, min ... | 2002 | 11927022 |
| european bat lyssavirus infection in spanish bat populations. | from 1992 to 2000, 976 sera, 27 blood pellets, and 91 brains were obtained from 14 bat species in 37 localities in spain. specific anti-european bat lyssavirus 1 (ebl1)-neutralizing antibodies have been detected in myotis myotis, miniopterus schreibersii, tadarida teniotis, and rhinolophus ferrumequinum in the region of aragon and the balearic islands. positive results were also obtained by nested reverse transcription-polymerase chain reaction on brain, blood pellet, lung, heart, tongue, and es ... | 2002 | 11971777 |
| lyssavirus infection of muscle spindles and motor end plates in striated muscle of hamsters. | immunofluorescent, light, and electron microscopy were used to document lyssavirus infection of muscle spindles and motor end plates. virus particles were seen in the narrow intercellular space between sensory nerve endings and intrafusal muscle fibers; they were also observed budding from intracellular and plasma membranes of the latter. involvement of motor nerves and motor end plates could only be demonstrated by electron microscopy. in nature, rabies virus invasion of the peripheral nervous ... | 1978 | 149526 |
| [the evaluation of a monoclonal antibody panel for lyssavirus typing in mexico]. | the purpose of this study was to evaluate the ability of a panel of eight antinucleocapsid monoclonal antibodies developed in europe to identify different strains of rabies virus isolated from a variety of animal species from diverse geographic areas in mexico. fifty-one virus-positive samples of brain tissue from various animal species and humans were studied. material from these samples was used to infect mice, whose brains were later tested by indirect immunofluorescence, using the monoclonal ... | 1995 | 8540994 |
| emerging viral infections in australia. | hendra virus infection should be suspected in someone with close association with horses or bats who presents acutely with pneumonia or encephalitis (potentially after a prolonged incubation period). australian bat lyssavirus infection should be suspected in a patient with a progressive neurological illness and a history of exposure to a bat. rabies vaccine and immunoglobulin should be strongly considered after a bite, scratch or mucous membrane exposure to a bat. japanese encephalitis vaccine s ... | 2002 | 12088481 |