| [perichordal necroses. contribution to the understanding of the spinal column morphogenesis]. | in 12--15 days old rat embryos and 12 days old mouse embryos vitally stained by acridin orange, a perichordal necrosis appears, showing metameric intensifications at the level of the dense sclerotomites. similar aspects were detected in serially sectioned rabbit and human embryos (6--7 mm c.r. length). the authors correlate the necrotic zones mentioned with the inductive functions of the notochord. | 1978 | 86154 |
| cross-reactions between haemophilus influenzae and nineteen other bacterial species. | forty-one different antigens were demonstrated in an antigen preparation obtained by sonication of a haemophilus influenzae (h. influenzae) type b strain, using crossed immunoelectrophoresis and antiserum obtained from rabbits. antigens were characterized by absorption experiments with whole heat-killed bacteria, temperature resistance and protein and polysaccharide staining. cross-reactions between h. influenzae type b and 19 other bacterial species were studied by various quantitative immunoel ... | 1979 | 94235 |
| muscle stretch and chemical muscle spindle excitation: effects on renshaw cells and efficiency of recurrent inhibition. | | 1976 | 137423 |
| [dynamics of sleep and the waking state after labyrinthine deafferentation]. | the author carried out studies on awake state and sleep (slow and paradoxal) after lybyrinth deafferentation. the states were evaluated by behavior, eeg, myogram and eye movements. the labyrinth defferentation was performed in combination (chemical and mechanical). bilateral labyrinth deafferentation reduced the duration of the awake state from 30% to 17%, p less than 0.001. the slow sleep was increased at the same time but there was no statistical significance. the parodoxal sleep was due both ... | 1976 | 179775 |
| radiometric detection of haemophilus in simulated blood cultures. | the radiometric detection of various haemophilus species was studied in simulated blood cultures and in blood culture media without added blood, but supplemented with nicotinamide-adenine dinucleotide (nad) or hemin, or both. haemophilus aphrophilus was the only species studied that was radiometrically detectable without added blood or nad. all other species studied (haemophilus influenzae, haemophilus parainfluenzae, haemophilus haemolyticus, and haemophilus parahaemolyticus) required either ad ... | 1979 | 218444 |
| cloning of restriction and modification genes in e. coli: the hbaii system from haemophilus haemolyticus. | the genes for a class ii restriction-modification system (hhaii) from haemophilus haemolyticus have been cloned in escherichia coli. the vector used for cloning was plasmid pbr322 which confers resistance to tetracycline and ampicillin and contains a single endonuclease r-psti site, (formula: see text), in the ampicillin gene. the procedure developed by bolivar et al. (1977) was used to form dna recombinants. h. haemolyticus dna was cleaved with psti endonuclease and poly(dc) extensions were add ... | 1978 | 350714 |
| characterization of plasmids from haemophilus parainfluenzae and haemophilus haemolyticus. | | 1979 | 384428 |
| chlamydial genital infections: a growing problem. | | 1979 | 478491 |
| the structure of brevistin. studies on antibiotics from the genus bacillus. x. | acid hydrolysis revealed the constituent amino acids by brevistin as follows: aspartic acid (d-form 2, l-form 1), l-threonine (1), glycine (1), sum of l-valine and l-isoleucine (1), l-phenylalanine (1), l-trytophan (1), and 2,4-diaminobutyric acid (d-form 1, l-form 1). the constituent fatty acid was elucidated to be anteisononanoic acid by gas chromatography-mass spectrometry. a lactone linkage was proved between phenylalanine and the hydroxy group of threonine. opening the lactone ring with dil ... | 1976 | 931806 |
| a specific endonuclease from haemophilus haemolyticus. | | 1976 | 1085372 |
| cleavage map of bacteriophage phix174 rf dna by restriction enzymes. | phix rf dna was cleaved by restriction enzymes from haemophilus influenzae rf (hinf i) and haemophilus haemolyticus (hha. i). twenty one fragments of approximately 25 to 730 base pairs were produced by hinf i and seventeen fragments of approximately 40 to 1560 base pairs by hha i. the order of these fragments has been established by digestion on haemophilus awgyptius (hae iii) and arthrobacter luteus (alu i) endonuclease fragments of phix rf with hinf i and hha1. by this method of reciprocal dig ... | 1976 | 1085927 |
| changes in vascular permeability associated with acetic acid-induced gastrin ulcer in rats. | gastric ulcer was induced in rats by application of acetic acid to the anterior wall of the stomach. leakage of circulating albumin into the gastric wall was estimated by intravenous injection of radioactive albumin and determination of the radioactivity in different samples of the stomach wall. the protein leakage was found to be markedly increased in the anterior wall of the stomach, the increase being most pronounced close to the ulcer. the leakage remained fairly constant during the first 10 ... | 1976 | 1248499 |
| quantitative study of striate single unit responses in monkeys performing an orientation discrimination task. | contour orientation discrimination accuracy is determined by the orientation bandwidth, response variance and response strength of single units that code for orientation. we measured the latter three properties for v1 cells of monkeys which were performing an orientation discrimination of the grating stimulating the cell under study. we recorded from 285 cells, of which 76% responded to the grating. the orientation bandwidth, measured as full width at half height of the tuning curve, varied over ... | 1991 | 1855551 |
| repeated community-based chemotherapy for the control of schistosoma mansoni: effect of screening and selective treatment on prevalences and intensities of infection. | the impact of repeated selective chemotherapy on prevalences and intensities of infection with schistosoma mansoni was evaluated in gihungwe transversals 1 and 2 (initial prevalence 60%) and buhandagaza/kizina (initial prevalence 35%), two village clusters in burundi. surveys were carried out at months -6, -3, 0, 3, 6, 9, 12, 24, and 36, with reference to the first intervention; treatment with praziquantel (40 mg/kg) was given at months 0, 12, 24, and 36 to subjects showing parasite eggs on a si ... | 1991 | 1951860 |
| diabetes-related changes in brain beta adrenoreceptors in rats as assessed by quantitative autoradiography: relationship to hypothalamic norepinephrine metabolism and pituitary-gonadal hormone secretion. | streptozotocin (stz)-induced diabetes produced significant and selective alterations in brain beta adrenoreceptor subtypes, norepinephrine (ne) metabolism and pituitary-testicular hormone in male rats. the densities of beta-1 but not beta-2 adrenoreceptors were increased in hypothalamus (anterior and lateral nuclei), thalamus (ventral posterior nucleus) and amygdala (basolateral nucleus) of the stz diabetic rats. in contrast, a decrease in the rate of metabolism of ne (estimated by the concentra ... | 1990 | 2168485 |
| comparison of the rim-h rapid identification kit with conventional tests for the identification of haemophilus spp. | a commercially available system, the rim-h system (austin biological laboratories, austin, tex.), was evaluated for its ability to rapidly and accurately identify various haemophilus spp. a total of 110 clinical isolates were tested by both the rim and conventional identification procedures. the rim agreed with the standard identification for 100% of the haemophilus influenzae (76 of 76) and 92.0% of the haemophilus parainfluenzae (23 of 25) isolates tested. the identifications of haemophilus pa ... | 1990 | 2203817 |
| evaluation of bcg at birth in the united arab emirates. | a total of 387 healthy full term infants who received bcg vaccination at birth were evaluated by the tuberculin ppd test at 6-8 weeks post vaccination. a total of 92% of these infants had visible bcg scars at that time. all infants were tested with ppd but only 264 returned for the test to be read. the tuberculin test was negative in 37 (14%) and of less than 5 mm induration in another 60 (23%) of the infants. the same pattern was observed in different nationalities. of 11 infants with no bcg sc ... | 1990 | 2238123 |
| evaluation of bcg at birth in the united arab emirates. | a total of 387 healthy full term infants who received bcg vaccination at birth were evaluated by the tuberculin ppd test at 6-8 weeks post vaccination. a total of 92% of these infants had visible bcg scars at that time. all infants were tested with ppd but only 264 returned for the test to be read. the tuberculin test was negative in 37 (14%) and of less than 5 mm induration in another 60 (23%) of the infants. the same pattern was observed in different nationalities. of 11 infants with no bcg sc ... | 1990 | 2238123 |
| mrna coding for oxytocin is present in axons of the hypothalamo-neurohypophysial tract. | neuronal mrna is thought to be restricted to perikaryal and dendritic compartments containing rough endoplasmic reticulum. we have used both in situ hybridization and dna polymerase chain reaction methods to determine the precise intracellular distribution of oxytocin mrna. using light- and electron-microscopic detection of in situ hybridization with 5'-bromo-2'-deoxyuridine-labeled oligonucleotide probes, we found oxytocin mrna in axons and herring bodies in the lateral and ventral hypothalamus ... | 1990 | 2268384 |
| [review of the present status of prevention, prophylaxis and therapy of pertussis and parapertussis]. | whooping cough is endemic throughout the world. it becomes epidemic every 4-5 years (yugoslavia 3-4 yrs). in europe its incidence ranges from 0.4 (hungary) to even 59/100.000 inhabitants (rumania; yugoslavia 28), with a general letality of 0.1% (infants: 1%; 75% children who die are younger than one yr). only 5-10% cases are supposed to be registered. a low socioeconomic status is more and more emphasized as the principal risk factor. its transmission rate is high (home contacts: 80-100%); infec ... | 1990 | 2292902 |
| [the role of veterinary toxicology in farm animals]. | the history of veterinary toxicology in farm animals, like that of human toxicology, is marked by a number of classical, large-scale cases of poisoning, which have become widely known. a number of these cases are briefly reported, attention being drawn to the specific problems to which they give rise. publicity, liability, diagnostic problems and amount of loss often have a delaying effect on settlements. methods which might improve this situation are broadly outlined. | 1990 | 2396243 |
| separation of branched sialylated oligosaccharides using high-ph anion-exchange chromatography with pulsed amperometric detection. | ten characterized sialylated oligosaccharides from bovine fetuin (b. bendiak, m. harris-brandts, s. w. michnick, j. p. carver, and d. a. cumming, biochemistry, in press; and d. a. cumming, c. g. hellerqvist, m. harris-brandts, s. w. michnick, j. p. carver, and b. bendiak, biochemistry, in press) were chromatographed using high-performance anion-exchange chromatography with pulsed amperometric detection. at near neutral ph values, oligosaccharides were separated according to their number of forma ... | 1989 | 2481411 |
| promoting activity of betel quid ingredients and their inhibition by retinol. | ingredients of betel quids, which have been linked to the high incidence of precancerous oral lesions and oral cancers, were examined for their promoting activity. aqueous extracts were tested using the bovine papillomavirus (bpv) dna transformation assay, which consists of cultured c3h/10t1/2 cells transfected with the plasmid pdpbv-1 as targets, and the frequency of transformed foci as endpoints. areca nut extracts enhanced the formation of bpv dna-induced transformed foci approximately tenfol ... | 1989 | 2713825 |
| [experimental validation of the expediency of the use of ultra-high frequency therapy in combination with antibacterial drugs in infiltrative tuberculosis of the lungs]. | the influence of the u.h.f. electric field in combination with specific antibacterial therapy on the character of morphological shifts, isoniazid accumulation, collagen fractional composition and tubercle bacilli population in the foci of infiltrative tuberculosis of the lungs was studied experimentally. the effect of this preformed physical factor on the process of tuberculosis was shown to be favourable. under the action of the u.h.f. electric field microcirculation in the lungs improved, and ... | 1989 | 2726711 |
| clinical evaluation of propofol as an intravenous anaesthetic agent in cats and dogs. | the clinical efficacy and safety of an emulsion containing 10 mg/ml of the intravenous anaesthetic propofol were evaluated in cats and dogs by veterinary surgeons in eight practices in the united kingdom. a total of 290 dogs and 207 cats were anaesthetised with propofol either as a single injection for procedures of short duration, or as an induction agent with maintenance provided by further incremental injections or as an induction agent with maintenance by gaseous agents. the mean induction d ... | 1989 | 2783794 |
| nuclear factor 1 (nf-1) binding sites in the genomes of human oncoviruses: a hypothetic role for reintegrated cellular origins of replication in malignant transformation. | we have found that genomes of human t cell leukemia-lymphoma virus type i (htlv-i), bk virus (bkv), and a hepatitis b virus (hbv) dna sequence integrated into dna of a hepatoma-derived cell line contain binding sites for nuclear factor 1 (nf-1), a cellular protein which binds to adenoviral and putative cellular origins of dna replication. we suggest that cellular origins of dna replication acquired by oncoviruses may play a role in malignant transformation after reintegration into the cellular g ... | 1988 | 2830475 |
| isolation of haem-bearing peptides of haem proteins by the use of adsorption chromatography. | | 1987 | 2832428 |
| [various indices of the immunologic reorganization of the body in allergic dermatitis]. | | 1987 | 2953139 |
| [primary leiomyosarcoma of the pulmonary artery. apropos of a case]. | a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ... | 1987 | 3122693 |
| pretreatment with icrf-187 allows a marked increase in the total cumulative dose of doxorubicin tolerated by beagle dogs. | to study the influence of icrf-187 on the functional and morphological effects of very large cumulative doses of doxorubicin, adult beagle dogs were given doxorubicin (1.75 mg/kg i.v.) either alone or 15 min after icrf-187 (25 mg/kg, i.v.) at 3-week intervals. control dogs received icrf-187 (25 mg/kg, i.v.) or 0.9% saline without doxorubicin. of eight animals receiving doxorubicin alone, two died after a total dose of 12.25 mg/kg and three died after 14 mg/kg; three others were in poor condition ... | 1988 | 3147886 |
| [laboratory and clinical studies of rokitamycin dry syrup in the field of pediatrics]. | laboratory and clinical studies on rokitamycin (rkm) dry syrup, a new macrolide antibiotic, were carried out in the field of pediatrics. the results are summarized as follows. 1. plasma concentrations and urinary recovery rates after oral administration on fasting of rkm dry syrup at doses of 10 mg/kg and 20 mg/kg to 2 and 1 cases, respectively, were determined. peak plasma levels were obtained in 30 minutes after administration of both dosages with half-lives of 1.5 to 2.2 hours. a clear-cut do ... | 1988 | 3172461 |
| the flavonoid silymarin increases the permeability of rat bone marrow cells to (3h)-uridine. | the pretreatment of rats with the flavonoid silymarin increases the in vitro uptake of (3h)-uridine to bone marrow cell rna. this stimulatory effect is unrelated to an increase in the activity of cellular polymerases. it is proposed that the flavonoid enhances the permeability of the cell to (3h)-uridine acting at the membrane level. | 1988 | 3187195 |
| overproduction and purification of the m.hhaii methyltransferase from haemophilus haemolyticus. | the hhaii methyltransferase gene from haemophilus haemolyticus was subcloned in an expression vector under control of the hybrid trp-lac promoter. induction with isopropyl-beta-d-thiogalactopyranoside results in overproduction of the methyltransferase to about 3% of total cellular protein. the methyltransferase was purified to near electrophoretic homogeneity by phosphocellulose, deae, and gel chromatography. its monomer mr by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is 25 kda, ... | 1988 | 3248721 |
| cytosine methylation as an effector of right-handed to left-handed dna structural transitions. | cytosine methylation has energetic and structural influences on left-handed z-dna formation in supercoiled plasmids. the restriction and modification enzymes from haemophilus haemolyticus (hhai and m.hhai) provide a system to locate and analyze small segments of z-dna in large supercoiled plasmids. an approach is outlined that uses m.hhai as an in vivo conformational probe for the detection of unusual dna structures in a living cell. also, characteristic features of the m.hhai gene and protein a ... | 1988 | 3266857 |
| nongonococcal urethritis: diagnosis and management. | | 1987 | 3295152 |
| crystal structure of enolase indicates that enolase and pyruvate kinase evolved from a common ancestor. | enolase or 2-phospho-d-glycerate hydrolase catalyses the dehydration of 2-phosphoglycerate to phosphoenolpyruvate, which in turn is converted by pyruvate kinase to pyruvate. we describe here the crystallographic determination of the structure of yeast enolase at high resolution (2.25 a) and an analysis of the structural homology between enolase, pyruvate kinase and triose phosphate isomerase. each of the two subunits of enolase forms two distinctive domains. the larger domain (residues 143-420) ... | 1988 | 3374614 |
| simultaneous cleft lip and palate repair: an experimental study in beagles. | this study was designed to test the hypothesis that simultaneous lip and palate repair results in more severe craniofacial growth aberrations than lip repair or palate repair performed separately. seventy-six purebred beagles were divided into five groups. two of these groups were controls (unoperated and unrepaired animals); the three remaining groups were experimental (in one group only the lip was repaired, in another only the palate was repaired, and in the last the lip and palate were repai ... | 1988 | 3380923 |
| criteria for a proof of migration routes of immature parasites inside hosts exemplified by studies of strongyloides ratti in the rat. | the first rigorous proof applicable to the migration pathway of an infective juvenile macroparasite inside its host is presented. third-stage larvae of a homogonic strain of strongyloides ratti applied in exact doses of less than 20 to the skin of the flank of young rats were recovered 16-40 h later in the naso-frontal part of the head. the peak proportion of the dose (po) recovered between 20 and 25 h in this site had a mean value of 0.316 +/- 0.021 in 48 animals. in 40 other rats infected simu ... | 1988 | 3405640 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| right-sided staphylococcus aureus endocarditis in intravenous drug abusers: two-week combination therapy. | to determine the efficacy of short-course combination regimens for selected cases of staphylococcus aureus endocarditis in intravenous drug abusers. | 1988 | 3421575 |
| distribution of noradrenaline in the brain of the teleost gasterosteus aculeatus l.: an immunohistochemical analysis. | the distribution of noradrenergic neurons in the brain of the three-spined stickleback was demonstrated with the indirect peroxidase-antiperoxidase (pap) immunohistochemical method with antibodies against a noradrenaline-bovine serum albumin conjugate. noradrenergic neuronal somata were exclusively located in the isthmal area of the brain stem and in the lower medulla. noradrenergic varicose axons innervate the reticular formation, motor nuclei, and interpeduncular nucleus of the brain stem, the ... | 1986 | 3540043 |
| cloning, sequencing, in vivo promoter mapping, and expression in escherichia coli of the gene for the hhai methyltransferase. | a 1476-base pair dna fragment from haemophilus haemolyticus containing the hhai methyltransferase gene was isolated from a cell library and cloned into pbr322. the nucleotide sequence of this fragment was determined. the structural gene is 981 nucleotides in length coding for a protein of 327 amino acids (mr 37,000). the translational start signal (atg) is preceded by the putative ribosome-binding site (taag). recombinant plasmids containing the 1476-basepair fragment are completely methylated w ... | 1987 | 3549710 |
| murine t-cell reactivity to cloned mycobacterium leprae antigens. | | 1986 | 3553803 |
| identification of the mutagenic quinoxaline isomers from fried ground beef. | two mutagens isolated from fried-beef patties were compared to a series of synthetic structural isomers of 2-aminodimethylimidazo[4,5-f]quinoxaline (meiqx) and 2-aminotrimethylimidao[4,5-f]quinoxaline (dimeiqx). comparison by nmr spectrometry and hplc coelution showed that one beef mutagen (molecular weight of 213) was identical to the 8-meiqx isomer not the 7-me isomer. another quinoxaline beef mutagen, having 3 methyl groups (molecular weight of 227), had an nmr spectrum different from the 5,8 ... | 1987 | 3553914 |
| a microplate enzyme-linked immunosorbent assay for measuring antibody to anaplasma marginale in cattle serum. | an enzyme-linked immunosorbent assay (elisa) method is described for measuring antibody against anaplasma marginale in cattle serum. this method was more sensitive and objective than a previously described elisa method for a. marginale and possible reasons for this are discussed. all 83 cattle experimentally infected with a. marginale (81) or a. centrale (2) developed demonstrable specific antibody but the serums of 98.8% of 839 cattle from cattle tick-free areas did not react by elisa; 378 seru ... | 1986 | 3729834 |
| hypercalcemia associated with malignancy in a horse. | hypercalcemia associated with malignancy was diagnosed in a 2-year-old thoroughbred filly admitted because of weight loss and reduced exercise tolerance of approximately 2 months' duration. laboratory findings included hypercalcemia, hypophosphatemia, anemia, marked neutrophilia with lymphopenia and eosinopenia, and normal immunoreactive parathyroid hormone concentration. at necropsy, a 53.6-kg tumor was located in the cranioventral aspect of the abdominal cavity. gross renal lesions were not no ... | 1986 | 3733508 |
| igg bearing covalently bound c3b has enhanced bactericidal activity for escherichia coli 0111. | the mechanism was sought by which bactericidal igg for e. coli 0111 (strain 12015) increases the bactericidal efficiency of c5b-9. igg did not affect the distribution of c3 deposition on the o-ag capsule and the outer membrane of 12015, suggesting that bactericidal igg was not directing complement activation to different sites on the bacterial surface. however, one-fifth of the c3 that was deposited in the presence of igg attached covalently to the antibody molecule. covalent complexes between p ... | 1985 | 3897448 |
| purification and characterization of two vascular endothelium effectors from fetal bovine retina, vitreous and serum. | the purification and characterization of uridine and thymidine, two previously isolated vascular endothelium effectors, are described. these two effectors were purified from all dialyzable fractions of fetal bovine serum (fbs), vitreous, and retina. the present study demonstrates the stimulatory characteristic of uridine and thymidine on the proliferation of aortic endothelial cells. the stimulation appears to link to their utilization by the cells in a dialyzed fbs-dependent manner. in the pres ... | 1985 | 4029288 |
| ipsilateral afferents to the commissural zone of the fascia dentata, demonstrated in decommissurated rats by silver impregnation. | | 1971 | 4106860 |
| histochemical observations on rumen mucosa structure and composition. | | 1969 | 4236739 |
| cytological analysis of the effect of methionine sulphoximine on protein metabolism of cells of the nervous system in vivo. | | 1968 | 4283665 |
| cytological analysis of the effect of methionine sulphoximine on protein metabolism of cells of the nervous system in vivo. | | 1968 | 4283665 |
| mechanism of adrenaline-induced lipolysis in adipose tissue. | | 1974 | 4363679 |
| preparation of glutamate dehydrogenase from micrococcus aerogenes. | | 1970 | 4397848 |
| studies on murine leprosy bacillus. x. studies on an atypical mycobacteria isolated from experimental murine leprosy mice. | | 1974 | 4449161 |
| purification of large crosslinked peptides from insoluble calf bone and skin collagens by hydroxyapatite chromatography. | | 1974 | 4473211 |
| conversion of human hexosaminidase a to hexosaminidase "b" by crude vibrio cholerae neuraminidase preparations: merthiolate is the active factor. | | 1974 | 4473220 |
| suppressor cells in the rabbit appendix. | | 1974 | 4547314 |
| effects of oxidized spermine and acrolein on the transforming activity of t4 dna. | | 1972 | 4552901 |
| effects of oxidized spermine and acrolein on the transforming activity of t4 dna. | | 1972 | 4552901 |
| [studies on murine leprosy bacillus. x. rapid growing mycobacteria isolated from experimental murine leprosy mice (author's transl)]. | | 1974 | 4617060 |
| isolation of a new rna polymerase-binding protein from sporulating bacillus subtilis. | rna polymerase was precipitated from extracts of radioactively labeled vegetative and sporulating bacillus subtilis with antiserum prepared against vegetative core polymerase. the precipitates were solubilized and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antiserum added to an extract of vegetative b. subtilis precipitated only the known subunits of core rna polymerase, but antiserum added to an extract of sporulating cells precipitated a new polypeptide of 70,000 da ... | 1973 | 4631355 |
| blood flow through different regions of long bone measured by a bone-seeking radioisotopic method. | | 1971 | 4923134 |
| hhai methylase and restriction endonuclease as probes for b to z dna conformational changes in d(gcgc) sequences. | the capacity of the modification methylase (mhhai) and restriction endonuclease (hhai) form haemophilus haemolyticus to methylate and cleave, respectively, recognition sites which are in right-handed b or left-handed z structures was determined in vitro. plasmids containing tracts of (dc-dg) as well as numerous individual d(gcgc) sites distributed around the vector were studied. negative supercoiling was used to convert the (dc-dg) tracts (approximately 30 bp in length) from a right-handed to a ... | 1984 | 6093048 |
| fimbriation of haemophilus species isolated from the respiratory tract of adults. | twenty-two clinical isolates of haemophilus species were studied within two passages of their original isolation for the presence of fimbriae by negative-staining electron microscopy. six isolates were identified as fimbriated, including three strains of nontypable haemophilus influenzae, one strain of haemophilus parainfluenzae, and two strains of haemophilus haemolyticus. in fresh isolates of fimbriated strains of nontypable h. influenzae, approximately 40%-50% of cells had fimbriae; after fiv ... | 1984 | 6146651 |
| the nucleotide sequence of is5 from escherichia coli. | a 3-kb fragment of haemophilus haemolyticus dna which carries the hhaii restriction (r) and modification (m) genes has been cloned into the psti site of pbr322 (mann et al., 1978). when propagated in escherichia coli, it was observed that spontaneous insertions of is5 inactivated the restriction gene, producing r- mutants at a frequency of 10(-6). electron microscopy, restriction-site mapping and sequence analysis of two r- plasmids have demonstrated the presence of is5 at a single target site i ... | 1981 | 6269959 |
| the nucleotide sequence of the hhaii restriction and modification genes from haemophilus haemolyticus. | we have determined the nucleotide sequence of a cloned 1710-bp segment of haemophilus haemolyticus dna which contains the hhaii restriction (r) and modification (m) genes. the m gene is 513 bp in length and the rgene is 681 bp in length. both are in the same reading frame, being separated by a 21-bp region. a ribosome-binding site is identified in front of each gene, but no haemophilus promoter is apparent on the cloned fragment. transcription originates from a plasmid promotor and proceeds in t ... | 1983 | 6315538 |
| relative proportions of haemophilus species in the throat of healthy children and adults. | to determine normal proportions of pharyngeal haemophilus species, qualitative and quantitative mapping of the species in the pharynx of ten healthy children and ten healthy adults was carried out using a selective and a non-selective medium. haemophilus organisms were present in all samples, comprising approximately 10% of the total cultivable flora (range 0.6-36.9%). haemophilus parainfluenzae was a member of the normal flora throughout life, constituting 74% of pharyngeal haemophilus organism ... | 1984 | 6332018 |
| binding of human hemoglobin by haemophilus influenzae. | binding of biotinylated human hemoglobin to haemophilus influenzae was detected when organisms were grown in heme-deplete, but not heme-replete, conditions. hemoglobin binding was completely inhibited by a 100-fold excess of unlabelled human hemoglobin or human hemoglobin complexed with human haptoglobin. binding was only partially inhibited by rat hemoglobin, bovine hemoglobin, human globin, and bovine globin, and not at all by heme, human serum albumin, bovine serum albumin, human transferrin, ... | 1994 | 8020748 |
| genetic characterization of a cryptic genospecies of haemophilus causing urogenital and neonatal infections. | in recent years, reports originating from several areas of the world have identified biotype iv strains of haemophilus influenzae as a cause of serious urogenital, neonatal, and mother-infant infections. preliminary analysis of a sample of biotype iv isolates found evidence for a cryptic genospecies of haemophilus (r. quentin, a. goudeau, r. j. wallace, jr., a. l. smith, r. k. selander, and j. m. musser. j. gen. microbiol. 136:1203-1209, 1990). eighteen biotype iv strains assigned to the cryptic ... | 1993 | 8099082 |
| functional roles of the conserved aromatic amino acid residues at position 108 (motif iv) and position 196 (motif viii) in base flipping and catalysis by the n6-adenine dna methyltransferase from thermus aquaticus. | the dna methyltransferase (mtase) from thermus aquaticus (m.taqi) catalyzes the transfer of the activated methyl group of s-adenosyl-l-methionine to the n6 position of adenine within the double-stranded dna sequence 5'-tcga-3'. to achieve catalysis m.taqi flips the target adenine out of the dna helix. on the basis of the three-dimensional structure of m.taqi in complex with the cofactor and its structural homology to the c5-cytosine dna mtase from haemophilus haemolyticus, tyr 108 and phe 196 we ... | 1999 | 9931007 |
| zebularine: a novel dna methylation inhibitor that forms a covalent complex with dna methyltransferases. | mechanism-based inhibitors of enzymes, which mimic reactive intermediates in the reaction pathway, have been deployed extensively in the analysis of metabolic pathways and as candidate drugs. the inhibition of cytosine-[c5]-specific dna methyltransferases (c5 mtases) by oligodeoxynucleotides containing 5-azadeoxycytidine (azadc) and 5-fluorodeoxycytidine (fdc) provides a well-documented example of mechanism-based inhibition of enzymes central to nucleic acid metabolism. here, we describe the int ... | 2002 | 12206775 |
| structural basis of icf-causing mutations in the methyltransferase domain of dnmt3b. | mutations in the gene encoding for a de novo methyltransferase, dnmt3b, lead to an autosomal recessive immunodeficiency, centromeric instability and facial anomalies (icf) syndrome. to analyse the protein structure and consequences of icf-causing mutations, we modelled the structure of the dnmt3b methyltransferase domain based on haemophilus haemolyticus protein in complex with the cofactor adomet and the target dna sequence. the structural model has a two-subdomain fold where the dna-binding re ... | 2002 | 12601140 |
| a classification of the hemolytic bacteria of the genus haemophilus: haemophilus haemolyticus bergey et al. and haemophilus parahaemolyticus nov spec. | | 1953 | 13069455 |
| presence of copper- and zinc-containing superoxide dismutase in commensal haemophilus haemolyticus isolates can be used as a marker to discriminate them from nontypeable h. influenzae isolates. | respiratory isolates of haemophilus haemolyticus are regularly misclassified as nontypeable (nt) haemophilus influenzae due to an aberrant hemolytic reaction on blood agar, with implications for treatment. the presence of sodc or its cognate protein, copper-zinc superoxide dismutase, can distinguish respiratory isolates of h. haemolyticus from nt h. influenzae with 100% accuracy. | 2006 | 16988021 |
| haemophilus haemolyticus: a human respiratory tract commensal to be distinguished from haemophilus influenzae. | haemophilus influenzae is a common pathogen in adults with chronic obstructive pulmonary disease (copd). in a prospective study, selected isolates of apparent h. influenzae had an altered phenotype. we tested the hypothesis that these variant strains were genetically different from typical h. influenzae. | 2007 | 17152011 |
| horizontal gene transfer of ftsi, encoding penicillin-binding protein 3, in haemophilus influenzae. | horizontal gene transfer has been identified in only a small number of genes in haemophilus influenzae, an organism which is naturally competent for transformation. this report provides evidence for the genetic transfer of the ftsi gene, which encodes penicillin-binding protein 3, in h. influenzae. mosaic structures of the ftsi gene were found in several clinical isolates of h. influenzae. to identify the origin of the mosaic sequence, complete sequences of the corresponding gene from seven type ... | 2007 | 17325223 |
| airway bacterial concentrations and exacerbations of chronic obstructive pulmonary disease. | increased bacterial concentration (load) in the lower airways and new bacterial strain acquisition have been posited as mechanisms for chronic obstructive pulmonary disease (copd) exacerbations. bacterial concentrations are higher during exacerbation than during stable disease; however, these studies are cross sectional and devoid of strain typing. | 2007 | 17478618 |
| pharyngeal colonization dynamics of haemophilus influenzae and haemophilus haemolyticus in healthy adult carriers. | haemophilus influenzae is an important cause of respiratory infections, including acute otitis media, sinusitis, and chronic bronchitis, which are preceded by asymptomatic h. influenzae colonization of the human pharynx. the aim of this study was to describe the dynamics of pharyngeal colonization by h. influenzae and an intimately related species, haemophilus haemolyticus, in healthy adults. throat specimens from four healthy adult carriers were screened for haemophilus species; 860 isolates we ... | 2007 | 17687018 |
| relationships of nontypeable haemophilus influenzae strains to hemolytic and nonhemolytic haemophilus haemolyticus strains. | haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while h. haemolyticus, the closest phylogenetic relative of h. influenzae, is arguably a strict pharyngeal commensal. a hemolytic phenotype has historically differentiated h. haemolyticus from h. influenzae, but the recent recognition of significant nonhemolytic h. haemolyticus colonization has decreased this trait's resolvability. given this and the potential of recombination between the species, we examined t ... | 2008 | 18039799 |
| delineation of the species haemophilus influenzae by phenotype, multilocus sequence phylogeny, and detection of marker genes. | to obtain more information on the much-debated definition of prokaryotic species, we investigated the borders of haemophilus influenzae by comparative analysis of h. influenzae reference strains with closely related bacteria including strains assigned to haemophilus haemolyticus, cryptic genospecies biotype iv, and the never formally validated species "haemophilus intermedius". multilocus sequence phylogeny based on six housekeeping genes separated a cluster encompassing the type and the referen ... | 2009 | 19060144 |
| detection of cryptic genospecies misidentified as haemophilus influenzae in routine clinical samples by assessment of marker genes fuck, hap, and sodc. | clinical isolates of haemophilus influenzae were assessed for the presence of fuck, hap, and sodc by hybridization with gene-specific probes, and isolates diverging from the expected h. influenzae genotype were characterized by phenotype and 16s rrna gene sequencing. two of 480 isolates were finally classified as variant strains ("nonhemolytic haemophilus haemolyticus"). | 2009 | 19535530 |
| a comparative study of the biological characters and pathogenesis of bacillus x (sternberg), bacillus icteroides (sanarelli), and the hog-cholera bacillus (salmon and smith). | 1. bacillus x (sternberg) belongs to the colon group. 2. bacillus icteroides (sanarelli) is a member of the hog-cholera group. 3. the various channels of infection, the duration of the disease and the gross and microscopical lesions in mice, guinea-pigs and rabbits are the same for bacillus icteroides and the hog-cholera bacillus. 4. the clinical symptoms and the lesions observed in dogs inoculated intravenously with bacillus icteroides, are reproduced in these animals by infection with the hog- ... | 1900 | 19866945 |
| studies in the common cold : i. observations of the normal bacterial flora of nose and throat with variations occurring during colds. | 1. the normal bacterial flora of the nose and throat of thirteen individuals has been studied over periods ranging from 5 to 9 months. 2. observations have been made of qualitative and quantitative changes in the flora occurring in the course of colds and of throat infections appearing in the group. 3. the normal basic nasal flora includes staphylococcus albus, diphtheroids, and for certain individuals staphylococcus aureus and citreus. occasional transient bacteria are gram-negative cocci and n ... | 1926 | 19869133 |
| a growth inhibitory substance for the influenza group of organisms in the blood of various animal species : the use of the blood of various animals as a selective medium for the detection of hemolytic streptococci in throat cultures. | 1. 5 per cent sheep blood agar is a selective medium for beta hemolytic streptococci in throat cultures since sheep blood inhibits the growth of bacillus x (h. hemolyticus) and b. parainfluenzae hemolyticus. the growth of h. influenzae is also inhibited by sheep blood. 2. the inhibitory action of sheep blood resides in the erythrocytes and is thermolabile. disruption of the cell by laking has no effect upon the inhibitor. 3. the bloods of animals closely related to the sheep, such as the goat an ... | 1938 | 19870730 |
| prevalence of the sodc gene in nontypeable haemophilus influenzae and haemophilus haemolyticus by microarray-based hybridization. | the sodc gene has been reported to be a useful marker for differentiating nontypeable (nt) haemophilus influenzae from haemophilus haemolyticus in respiratory-tract samples, but discrepancies exist as to the prevalence of sodc in nt h. influenzae. therefore, we used a microarray-based, "library-on-a-slide" method to differentiate the species and found that 21 of 169 (12.4%) nt h. influenzae strains and all 110 (100%) h. haemolyticus strains possessed the sodc gene. multilocus sequence analysis c ... | 2010 | 20042621 |
| antimicrobial characterisation of cem-101 activity against respiratory tract pathogens, including multidrug-resistant pneumococcal serogroup 19a isolates. | cem-101 is a novel fluorinated macrolide-ketolide with potent activity against bacterial pathogens that are susceptible or resistant to other macrolide-lincosamide-streptogramin b (mls(b))-ketolide agents. cem-101 is being developed for oral and parenteral use in moderate to moderately severe community-acquired bacterial pneumonia. the objective of this study was to assess the activity of cem-101 and comparators against contemporary respiratory tract infection (rti) isolates. a worldwide sample ... | 2010 | 20211548 |
| haemophilus influenzae and haemophilus haemolyticus in tonsillar cultures of adults with acute pharyngotonsillitis. | the aim of this study was to evaluate the clinical implication of haemophilus haemolyticus, one of the closest relative of haemophilus influenzae, on acute pharyngotonsillitis. | 2010 | 20392581 |
| prevalence of haemophilus influenzae type b genetic islands among clinical and commensal h. influenzae and h. haemolyticus isolates. | five genetic islands (higi) found in haemophilus influenzae type b strain eagan were used as hybridization probes on type b, haemophilus haemolyticus, and nontypeable h. influenzae (nthi) isolates. higi2 and higi7 were significantly more prevalent in nthi isolates from children with otitis media than in those from the throats of healthy children. | 2010 | 20463164 |
| nasopharyngeal carriage of haemophilus haemolyticus in otitis-prone and healthy children. | haemophilus haemolyticus is often incorrectly categorized as nontypeable haemophilus influenzae (nthi) upon culture. pcr analyses of 266 nthi-like nasopharyngeal isolates from children with and without recurrent acute otitis media (raom) revealed that 11.7% were h. haemolyticus and 9.4% gave equivocal results. children with raom were more likely to carry h. haemolyticus. | 2010 | 20463165 |
| haemophilus influenzae outer membrane protein p6 molecular characterization may not differentiate all strains of h. influenzae from h. haemolyticus. | distinguishing nontypeable haemophilus influenzae and haemophilus haemolyticus isolates by outer membrane protein (omp) p6 gene sequencing is complicated by sequence variants in isolates. further testing using rapid nh and multilocus sequence analysis may not help identify some isolates. translated omp p6 gene sequences are not conserved among all isolates presumed to be h. influenzae. | 2010 | 20686092 |
| prevalence of genetic differences in phosphorylcholine expression between nontypeable haemophilus influenzae and haemophilus haemolyticus. | although non-typeable (nt) haemophilus influenzae and haemophilus haemolyticus are closely related human commensals, h. haemolyticus is non-pathogenic while nt h. influenzae is an important cause of respiratory tract infections. phase-variable phosphorylcholine (chop) modification of lipooligosaccharide (los) is a nt h. influenzae virulence factor that, paradoxically, may also promote complement activation by binding c-reactive protein (crp). crp is known to bind more to chop positioned distally ... | 2010 | 21073698 |
| medical microbiology | the genus haemophilus includes a number of species that cause a wide variety of infections but share a common morphology and a requirement for blood-derived factors during growth that has given the genus its name. haemophilus influenzae, the major pathogen, can be separated into encapsulated or typable strains, of which there are seven types (a through f including e') based on the antigenic structure of the capsular polysaccharide, and unencapsulated or nontypable strains. type b h influenzae is ... | 1996 | 21413340 |
| genome sequences for five strains of the emerging pathogen haemophilus haemolyticus. | we report the first whole-genome sequences for five strains, two carried and three pathogenic, of the emerging pathogen haemophilus haemolyticus. preliminary analyses indicate that these genome sequences encode markers that distinguish h. haemolyticus from its closest haemophilus relatives and provide clues to the identity of its virulence factors. | 2011 | 21952546 |
| frequency of hepatitis b antigen in blood donors. | routine testing of all blood donations for hepatitis b antigen by counter-immunoelectrophoresis was started in april 1971. the frequency of hbag carriers among apparently healthy volunteer blood donors in the toronto centre of the canadian red cross blood transfusion service was 15 in 10,000 with a male:female ratio of 6:1. excluding penitentiary inmates, the highest incidence was among young, male, non-anglo-saxon city dwellers.a system of notification of hbag-positive donors was developed, thr ... | 2015 | 4689555 |
| changes in the incidence of fracture of the upper end of the humerus during a 30-year period. a study of 2125 fractures. | the incidence of fracture of the upper end of the humerus by age group and sex was studied for a 32-year period from 1950 to 1982. the following numbers of humeral fractures occurred: 1950-1954, 411 fractures; 1961-1964, 552 fractures; 1971-1973, 639 fractures; and 1981-1982, 523 fractures. there was a progressive increase in the age-related incidence in older persons, especially in women. there was a higher incidence of fractures classified as severe in the 1980s compared with the 1950s. | 2012 | 3370872 |
| a randomized phase iii trial of cisplatinum, methotrexate, cisplatinum + methotrexate, and cisplatinum + 5-fluoro-uracil in end-stage head and neck cancer. | in a previous phase iii trial of chemotherapy for patients with end-stage squamous cell carcinoma of the head and neck we have shown that bleomycin shortened the survival period, compared with untreated controls. the survival of patients treated with cisplatinum and bleomycin did not differ significantly from untreated patients, but cisplatinum significantly prolonged survival. a further similar trial is reported here, the four treatment arms were methotrexate alone, cisplatinum alone, cisplatin ... | 2012 | 3303823 |
| high prevalence of trichomona infections in adolescents. | | 2010 | 2910629 |
| bupivacaine disposition in mother, fetus, and neonate after spinal anesthesia for cesarean section. | uptake of bupivacaine from the subarachnoid space and its placental transfer were measured in six patients undergoing elective cesarean section. maternal plasma levels (59 +/- 32 ng/ml) were only about 5% of those found in a comparable previous study of epidural anesthesia. mean plasma umbilical venous bupivacaine levels (20.2 +/- 21 ng/ml) were 7% of those found after epidural anesthesia. mean umbilical venous/maternal venous bupivacaine ratios were 0.34 +/- 0.12 and mean umbilical arterial/umb ... | 2013 | 3578847 |
| an improved method for the simultaneous determination of morphine and its principal glucuronide metabolites. | | 2011 | 3235512 |