| The viral RNA-based transfection of enhanced green fluorescent protein (EGFP) in the parasitic protozoan Trichomonas vaginalis. | Here we have developed methods to transiently and stably transfect the human pathogenic protist Trichomonas vaginalis. The viral RNA-based transfection vector pTVV-EGFP/NEO was constructed by using enhanced green fluorescent protein gene (EGFP) and neomycin resistance gene (NEO) in tandem to replace the whole gene encoding region of T. vaginalis virus (TVV). The in vitro transcripts of linearized pTVV-EGFP/NEO were electroporated into trophozoites and the transfectants transiently expressed EGFP ... | 2011 | 21861063 |
| [cloning and sequence analysis of a partial gene of trichomonas vaginalis dsrna virus]. | a pair of degenerate primers were designed following the published nucleotide sequence of trichomonas vaginalis virus(u08999, nc003873, nc003824, nc003834). using the total nucleic acids extracted from the trichomonas vaginalis as template, rt-pcr was performed with the primers to obtain a fragment of the tvv. the product was cloned, sequenced and compared with the sequences available in the genbank. the size of the amplified gene was 1 454bp, which shares 82.9% sequence identity with the tricho ... | 2006 | 17361826 |
| double-stranded rna viral infection in cuban trichomonas vaginalis isolates. | trichomonas vaginalis can be infected with double-stranded rna (dsrna) viruses designated t. vaginalis virus (tvv), which may have important implications for trichomonal virulence and disease pathogenesis. we tested for tvv in 40 fresh t. vaginalis isolates from cuban patients by total extraction of nucleic acids (dna and rna). tvv was detected in 22 (55%) of the 40 t. vaginalis isolates. this gives an estimate of the infection rate of cuban t. vaginalis isolates by the dsrna virus. future resea ... | 2006 | 16410949 |
| identification of a satellite double-stranded rna in the parasitic protozoan trichomonas vaginalis infected with t. vaginalis virus t1. | co-infection by a 0.5-kb small double-stranded (ds) rna together with trichomonas vaginalis virus (tvv) genomic 4.6-kb dsrna is commonly observed in a number of t. vaginalis isolates. by molecular cloning and primer extension experiments, the 497-bp cdna sequence of a 0.5-kb dsrna co-infecting with tvv-t1 in t vagina/is t1 isolate was elucidated. consistent with the replication cycle of a typical dsrna virus, a plus-strand viral rna beginning at +1 of the 0.5-kb dsrna was identified in infected ... | 1995 | 11831699 |
| the complete cdna sequence of a type ii trichomonas vaginalis virus. | trichomonas vaginalis viruses (tvv), which may regulate p270 gene expression in the protozoan pathogen t. vaginalis, are a group of divergent double-stranded (ds) rna viruses. in the present study, the complete 4674-bp cdna sequence of a 4.6-kb ds rna from a newly identified tvv2-1 isolate was determined. the sequence of the plus-strand mrna contains four open reading frames, which encode overlapping cap and pol genes in the reading frame 2 and reading frame 1, respectively, and two putative ser ... | 2000 | 10662630 |
| double stranded rna virus in south african trichomonas vaginalis isolates. | to screen trichomonas vaginalis isolates from south africa for the presence of a small double stranded rna virus designated t vaginalis virus (tvv). | 2003 | 12835302 |