| functional genomic studies of the intestinal response to a foodborne enteropathogen in a humanized gnotobiotic mouse model. | members of the genus listeria provide a model for defining host responses to invasive foodborne enteropathogens. active translocation of listeria monocytogenes across the gut epithelial barrier is mediated by interaction of bacterial internalin (inla) and its species-specific host receptor, e-cadherin, whereas translocation across peyer's patches through m-cells is inla-independent. to define microbial determinants and molecular correlates of the host response to translocation via these two rout ... | 2007 | 17389602 |
| bacterial ligands generated in a phagosome are targets of the cytosolic innate immune system. | macrophages are permissive hosts to intracellular pathogens, but upon activation become microbiocidal effectors of innate and cell-mediated immunity. how the fate of internalized microorganisms is monitored by macrophages, and how that information is integrated to stimulate specific immune responses is not understood. activation of macrophages with interferon (ifn)-gamma leads to rapid killing and degradation of listeria monocytogenes in a phagosome, thus preventing escape of bacteria to the cyt ... | 2007 | 17397264 |
| prevalence and growth of listeria monocytogenes in naturally contaminated cold-smoked salmon. | to investigate listeria monocytogenes contamination and behaviour in naturally contaminated french cold-smoked salmon (css). | 2007 | 17397479 |
| cutting edge: recombinant listeria monocytogenes expressing a single immune-dominant peptide confers protective immunity to herpes simplex virus-1 infection. | the vast majority of the world's population is infected with hsv. although antiviral therapy can reduce the incidence of reactivation and asymptomatic viral shedding, and limit morbidity and mortality from active disease, it cannot cure infection. therefore, the development of an effective vaccine is an important global health priority. in this study, we demonstrate that recombinant listeria monocytogenes (lm) expressing the h-2k(b) glycoprotein b (gb)(498-505) peptide from hsv-1 triggers a robu ... | 2007 | 17404252 |
| beta1-adrenergic receptors on immune cells impair innate defenses against listeria. | cold restraint (cr) for 1 h elicits a psychological and physiological stress that inhibits host defenses against listeria monocytogenes (lm). previous analyses indicated that this inhibition is not due to depletion of b or t cells but is instead dependent on signaling through beta-adrenoceptors (betaars). we now show that impaired host resistance by cr cannot be accounted for by a decrease in lm-specific (listeriolysin o(91-99) tetramer(+)) effector cd8(+) t cells; this result is consistent with ... | 2007 | 17404268 |
| myd88-dependent activation of b220-cd11b+ly-6c+ dendritic cells during brucella melitensis infection. | ifn-gamma is a key cytokine controlling brucella infection. one of its major function is the stimulation of brucella-killing effector mechanisms, such as inducible no synthase (inos)/nos2 activity, in phagocytic cells. in this study, an attempt to identify the main cellular components of the immune response induced by brucella melitensis in vivo is made. ifn-gamma and inos protein were analyzed intracellularly using flow cytometry in chronically infected mice. although tcrbeta(+)cd4(+) cells wer ... | 2007 | 17404301 |
| [one episode of meningoencephalitis, three diagnoses]. | meningo-encephalitis is a set of threatening diseases. the treatment needs to be started quickly for pathogens such as herpes simplex virus type 1 or listeria monocytogenes. apart from these classical etiologies, many other diseases may induce meningo-encephalitis. we report the case of a patient, infected with hiv, who presented a history of meningo-encephalitis due to herpes simplex type 1. three weeks later, he presented an encephalopathy due to aciclovir and then we discovered a chronic meni ... | 2007 | 17404526 |
| [survey of the presence of listeria monocytogenes in meat products sold in retail]. | the present study evaluates the presence of listeria spp and particularly of l. monocytogenes in bovine, pork and poultry meats sold by retail in supermarkets and butchers in the city of cassino. the sensibility to the antibiotics mostly used in the veterinary practice has been tested on the isolated strains. the different species of listeria have shown a considerable variation of isolation based on the meat's typology and on the different store's provenance. moreover our results show greater de ... | 2007 | 17405508 |
| a rare case of brainstem encephalitis by listeria monocytogenes with isolated mesencephalic localization. case report and review. | we describe a case of brainstem infection by listeria monocytogenes with right oculomotor palsy and lip drop, facial hypoesthesia, left arm paresthesia, positive blood culture, and sterile liquor in a 63-year-old man. magnetic resonance imaging revealed an isolated mesencephalic lesion. localization of this kind accounted for 3% of 111 cases reviewed. | 2007 | 17408902 |
| brain-stem listeriosis: a comparison of spect and mri findings. | listeria monocytogenes, although uncommon as a cause of illness in the general population, can result in serious illness when it affects pregnant women, neonates, the elderly, and immunocompromised individuals. typically, it is a food-borne organism. this report describes a case of brain-stem listeriosis in a previously healthy 51-year-old woman. the diagnosis was based on clinical findings, the results of cerebrospinal fluid (csf) analysis, csf culture, and magnetic resonance imaging (mri) find ... | 2006 | 17415328 |
| susceptibility of listeria monocytogenes strains isolated from dairy products and frozen vegetables to antibiotics inhibiting murein synthesis and to disinfectants. | the susceptibility of 96 strains of listeria monocytogenes isolated from food to antibiotics and disinfectants currently used in human therapy, veterinary, medicine and food industry was determined by a standard operating procedure--broth dilution method. antimicrobial agents included the beta-lactams ampicillin and penicillin, the lantibiotic nisin, and the disinfectants benzalkonium chloride and chlorhexidine gluconate. among the studied strains we found 13 strains with 8-fold, 7 strains with ... | 2006 | 17416064 |
| flagellar motility is critical for listeria monocytogenes biofilm formation. | the food-borne pathogen listeria monocytogenes attaches to environmental surfaces and forms biofilms that can be a source of food contamination, yet little is known about the molecular mechanisms of its biofilm development. we observed that nonmotile mutants were defective in biofilm formation. to investigate how flagella might function during biofilm formation, we compared the wild type with flagellum-minus and paralyzed-flagellum mutants. both nonmotile mutants were defective in biofilm develo ... | 2007 | 17416647 |
| identification of the sigmab regulon of bacillus cereus and conservation of sigmab-regulated genes in low-gc-content gram-positive bacteria. | the alternative sigma factor sigma(b) has an important role in the acquisition of stress resistance in many gram-positive bacteria, including the food-borne pathogen bacillus cereus. here, we describe the identification of the set of sigma(b)-regulated genes in b. cereus by dna microarray analysis of the transcriptome upon a mild heat shock. twenty-four genes could be identified as being sigma(b) dependent as witnessed by (i) significantly lower expression levels of these genes in mutants with a ... | 2007 | 17416654 |
| entry of the bacterial pathogen listeria monocytogenes into mammalian cells. | the bacterial pathogen listeria monocytogenes causes food-borne illnesses leading to meningitis or abortion. listeria provokes its internalization ('entry') into mammalian cells that are normally non-phagocytic, such as intestinal epithelial cells and hepatocytes. entry provides access to a nutrient-rich cytosol and allows translocation across anatomical barriers. here i discuss the two major internalization pathways used by listeria. these pathways are initiated by binding of the bacterial surf ... | 2007 | 17419717 |
| the multiple mechanisms of ca2+ signalling by listeriolysin o, the cholesterol-dependent cytolysin of listeria monocytogenes. | cholesterol-dependent cytolysins (cdcs) represent a large family of conserved pore-forming toxins produced by several gram-positive bacteria such as listeria monocytogenes, streptococcus pyrogenes and bacillus anthracis. these toxins trigger a broad range of cellular responses that greatly influence pathogenesis. using mast cells, we demonstrate that listeriolysin o (llo), a prototype of cdcs produced by l. monocytogenes, triggers cellular responses such as degranulation and cytokine synthesis i ... | 2007 | 17419718 |
| jinx, an mcmv susceptibility phenotype caused by disruption of unc13d: a mouse model of type 3 familial hemophagocytic lymphohistiocytosis. | mouse cytomegalovirus (mcmv) susceptibility often results from defects of natural killer (nk) cell function. here we describe jinx, an n-ethyl-n-nitrosourea-induced mcmv susceptibility mutation that permits unchecked proliferation of the virus, causing death. in jinx homozygotes, activated nk cells and cytotoxic t lymphocytes (ctls) fail to degranulate, although they retain the ability to produce cytokines, and cytokine levels are markedly elevated in the blood of infected mutant mice. jinx was ... | 2007 | 17420270 |
| listeria monocytogenes from a nova scotia moose. | | 1960 | 17421180 |
| septicemia due to listeria monocytogenes in a newborn foal. | | 1987 | 17422889 |
| a case of bovine mastitis caused by listeria monocytogenes. | | 1990 | 17423692 |
| dimeric structure of the cell shape protein mrec and its functional implications. | the bacterial actin homologue mreb forms helical filaments in the cytoplasm of rod-shaped bacteria where it helps maintain the shape of the cell. mreb is co-transcribed with mrec that encodes a bitopic membrane protein with a major periplasmic domain. like mreb, mrec is localized in a helical pattern and might be involved in the spatial organization of the peptidoglycan synthesis machinery. here, we present the structure of the major, periplasmic part of mrec from listeria monocytogenes at 2.5 a ... | 2006 | 17427287 |
| multiplexed identification of blood-borne bacterial pathogens by use of a novel 16s rrna gene pcr-ligase detection reaction-capillary electrophoresis assay. | we have developed a novel high-throughput pcr-ligase detection reaction-capillary electrophoresis (pcr-ldr-ce) assay for the multiplexed identification of 20 blood-borne pathogens (staphylococcus epidermidis, staphylococcus aureus, bacillus cereus, enterococcus faecalis, enterococcus faecium, listeria monocytogenes, streptococcus pneumoniae, streptococcus pyogenes, streptococcus agalactiae, escherichia coli, klebsiella pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, acinetobacter bau ... | 2007 | 17428930 |
| longitudinal monitoring of listeria monocytogenes contamination patterns in a farmstead dairy processing facility. | contamination of dairy products with listeria monocytogenes is a concern because multiple human listeriosis outbreaks have been linked to contaminated cheese and dairy products. dairy production on farmstead operations may be a particular concern because l. monocytogenes is also an animal pathogen that can be shed by ruminants with and without clinical symptoms; physical proximity between production animal and dairy processing facilities may thus provide a higher risk for introduction of l. mono ... | 2007 | 17430956 |
| the resistance to detachment of dairy strains of listeria monocytogenes from stainless steel by shear stress is related to the fluid dynamic characteristics of the location of isolation. | strains of listeria monocytogenes isolated from artisanal portuguese cheese-making dairies were divided into two categories on the basis of the locations from which they were isolated: strains from dynamic locations were those that were habitually exposed to flowing liquids during the process of cheese-making, whereas those from static locations were rarely, if ever, exposed to the shear stresses generated by liquid flows. the strength of attachment to stainless steel discs of all of these strai ... | 2007 | 17433481 |
| gut osmolarity: a key environmental cue initiating the gastrointestinal phase of listeria monocytogenes infection? | in addition to the severe invasive systemic disease of listeriosis, recent evidence suggests that the gram-positive intracellular bacterial pathogen listeria monocytogenes is also a causative agent of febrile gastroenteritis. we examined the listerial response to stresses normally encountered in the upper small intestine and demonstrate that osmotic stress appears to be at the top of the hierarchy of stress responses during gastrointestinal residence. furthermore, we suggest that the increased o ... | 2007 | 17433559 |
| a subacute bacterial endocarditis in a patient with aortic prosthetic valve due to listeria monocytogenes presenting with perivalvular leak. | | 2007 | 17434622 |
| germicidal ultraviolet light to lower numbers of listeria monocytogenes on broiler breast fillets. | raw broiler breast fillets were subjected to germicidal ultraviolet (uv) light (dose of 1,000 microw/cm(2) for 5 min at a wavelength of 254 nm) to evaluate its potential to reduce listeria monocytogenes numbers on raw product before shipment to a poultry further-processing plant. boneless, skinless breast fillets were inoculated with 4 different strains of l. monocytogenes 5 min before treatment. after the uv treatment, breast fillets were stored at 4 degrees c for 24 h. enumeration of remaining ... | 2007 | 17435033 |
| some essential elements on the inlc promoter for prfa-dependent regulation in listeria monocytogenes. | to study some essential elements of a prfa-dependent promoter of listeria monocytogenes, a series of promoter mutants incorporated into upstream of a promoterless lacz gene were constructed from a known listerial prfa-dependent promoter, inlc promoter, by pcr-mediated site-directed mutagenesis and recombinant pcr technique and then electroporated into l. monocytogenes wild-type strain p14, prfa * mutant pl4a and prfa deletion mutant a42. the corresponding transcription activities of altered prom ... | 2007 | 17436618 |
| characteristics of cell-mediated, anti-listerial immunity induced by a naturally avirulent listeria monocytogenes serotype 4a strain hcc23. | the characteristics of cell-mediated, anti-listerial immune response initiated by an avirulent listeria monocytogenes serotype 4a strain hcc23 was assessed. similar to virulent strain egd, avirulent strain hcc23 grew readily within macrophage-like j774 cells, but nonhemolytic strain atcc 15313 did not. compared with egd, hcc23 induced a relatively low level of gamma interferon (ifn-gamma) in mice, and atcc 15313 stimulated no detectable ifn-gamma. the percentages of gated cd4 t cells from mice i ... | 2007 | 17437086 |
| memory cd4 t cells enhance primary cd8 t-cell responses. | cd4 t-cell help is required for optimal memory cd8 t-cell responses. we have found that engaging preexisting cd4 th1, but not th2, memory cells at the time of cd8 t-cell priming results in increased cd8 effector responses to both bacterial and viral pathogens. the enhanced responses are characterized by increased numbers of cytokine-producing, antigen-specific cells. these findings suggest that engaging endogenous memory th1 cells may increase cellular responses in an immunotherapy or vaccinatio ... | 2007 | 17438031 |
| antigen presentation by nonhemopoietic cells amplifies clonal expansion of effector cd8 t cells in a pathogen-specific manner. | professional apcs of hemopoietic-origin prime pathogen-specific naive cd8 t cells. the primed cd8 t cells can encounter ag on infected nonhemopoietic cell types. whether these nonhemopoietic interactions perpetuate effector t cell expansion remains unknown. we addressed this question in vivo, using four viral and bacterial pathogens, by comparing expansion of effector cd8 t cells in bone marrow chimeric mice expressing restricting mhc on all cell types vs mice that specifically lack restricting ... | 2007 | 17442964 |
| anthrax lethal toxin paralyzes actin-based motility by blocking hsp27 phosphorylation. | inhalation of anthrax causes fatal bacteremia, indicating a meager host immune response. we previously showed that anthrax lethal toxin (lt) paralyzes neutrophils, a major component of innate immunity. here, we have found that lt also inhibits actin-based motility of the intracellular pathogen listeria monocytogenes. lt inhibition of actin assembly is mediated by blockade of hsp27 phosphorylation, and can be reproduced by treating cells with the p38 mitogen-activated protein (map) kinase inhibit ... | 2007 | 17446863 |
| the in vitro antibacterial activity of dietary spice and medicinal herb extracts. | the in vitro antibacterial activities of a total of 46 extracts from dietary spices and medicinal herbs were investigated by agar-well diffusion method against five foodborne bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli, and salmonella anatum). their total phenolic contents were also evaluated. many herb and spice extracts contained high levels of phenolics and exhibited antibacterial activity against foodborne pathogens. gram-positive bacteria were ... | 2007 | 17449125 |
| insufficient differentiation of live and dead campylobacter jejuni and listeria monocytogenes cells by ethidium monoazide (ema) compromises ema/real-time pcr. | recently, ethidium monoazide (ema) has been proposed as a means of reducing the real-time pcr signal originating from free dna and dead bacterial cells by selectively entering damaged cells and blocking the dna for pcr amplification via photoactivation. the present study investigated the effect of ema on viable and dead bacterial cells using real-time pcr, plate count method and microscopy. the foodborne pathogens campylobacter jejuni and listeria monocytogenes were used as a gram-negative and a ... | 2007 | 17449228 |
| recurrent and sporadic listeria monocytogenes contamination in alheiras represents considerable diversity, including virulence-attenuated isolates. | microbiological characterization of alheiras, traditional smoked meat sausages produced in northern portugal, had previously shown that more than 60% of the lots analyzed were contaminated with listeria monocytogenes at levels higher than 100 cfu/g. in order to better understand l. monocytogenes contamination patterns in alheiras, we characterized 128 l. monocytogenes isolates from alheiras using a variety of subtyping techniques (i.e., molecular serotyping; arsenic, cadmium, and tetracycline re ... | 2007 | 17449681 |
| short peptides derived from the nh2-terminus of subclass iia bacteriocin enterocin crl35 show antimicrobial activity. | subclass iia bacteriocins are characterized by a hydrophilic n-terminal domain that shares a ygngvxcxxxxc consensus and a variable hydrophobic c-terminus. enterocin crl35 is a 43-amino-acid heat stable peptide with antilisterial activity. short synthetic peptides derived from the n-terminal half of enterocin crl35 and other subclass iia bacteriocins were evaluated for antimicrobial properties. | 2007 | 17449885 |
| an oral salmonella vaccine promotes the down-regulation of cell surface toll-like receptor 4 (tlr4) and tlr2 expression in mice. | a single oral immunization with the lon-protease-deficient salmonella enterica serovar typhimurium (strain cs2022) induced protective immunity in mice against a subcutaneous challenge with virulent listeria monocytogenes as well as virulent salmonella serovar typhimurium. the populations of cell surface toll-like receptor 4 (tlr4) and tlr2 on peritoneal macrophages decreased at week 6 after immunization. this population decrease was not reversed after a challenge with either salmonella or lister ... | 2007 | 17451442 |
| adapter protein sh2-bbeta stimulates actin-based motility of listeria monocytogenes in a vasodilator-stimulated phosphoprotein (vasp)-dependent fashion. | sh2-bbeta (src homology 2 bbeta) is an adapter protein that is required for maximal growth hormone-dependent actin reorganization in membrane ruffling and cell motility. here we show that sh2-bbeta is also required for maximal actin-based motility of listeria monocytogenes. sh2-bbeta localizes to listeria-induced actin tails and increases the rate of bacterial propulsion in infected cells and in cell extracts. furthermore, listeria motility is decreased in mouse embryo fibroblasts from sh2-b(-/- ... | 2007 | 17452473 |
| type i interferon signaling is required for activation of the inflammasome during francisella infection. | francisella tularensis is a pathogenic bacterium whose virulence is linked to its ability to replicate within the host cell cytosol. entry into the macrophage cytosol activates a host-protective multimolecular complex called the inflammasome to release the proinflammatory cytokines interleukin (il)-1beta and -18 and trigger caspase-1-dependent cell death. in this study, we show that cytosolic f. tularensis subspecies novicida (f. novicida) induces a type i interferon (ifn) response that is essen ... | 2007 | 17452523 |
| diffusion-weighted magnetic resonance imaging in rhombencephalitis due to listeria monocytogenes. | we present diffusion-weighted imaging findings of a case of rhombencephalitis due to listeria monocytogenes. it is a rare, life-threatening disorder. the diagnosis is difficult by clinical findings only. in this report, we aim to draw attention to the role of conventional and diffusion-weighted magnetic resonance imaging findings. to our knowledge, this is the first case report in the literature with apparent diffusion coefficient values of diseased brain parenchyma. | 2007 | 17453530 |
| suppression of phagocytic and bactericidal functions of rat alveolar macrophages by the organic component of diesel exhaust particles. | exposure to diesel exhaust particles (dep) was shown to increase the susceptibility of the lung to bacterial infection in rats. in this study, the effects of dep on alveolar macrophage (am) phagocytic and bactericidal functions and cytokine secretion by am and lymphocytes in response to listeria monocytogenes infection were investigated in vitro and the roles of different dep components in these processes were compared. exposure to dep or the organic extracts of dep (edep) significantly decrease ... | 2007 | 17454558 |
| bacteriocin production as a mechanism for the antiinfective activity of lactobacillus salivarius ucc118. | the mechanisms by which probiotic strains enhance the health of the host remain largely uncharacterized. here we demonstrate that lactobacillus salivarius ucc118, a recently sequenced and genetically tractable probiotic strain of human origin, produces a bacteriocin in vivo that can significantly protect mice against infection with the invasive foodborne pathogen listeria monocytogenes. a stable mutant of lb. salivarius ucc118 that is unable to produce the abp118 bacteriocin also failed to prote ... | 2007 | 17456596 |
| necrotizing encephalitis of unknown cause in fennoscandian arctic foxes (alopex lagopus). | a neurologic disease affected a colony of endangered fennoscandian arctic foxes (alopex lagopus) kept in captivity for breeding purposes. several outbreaks of disease occurred between 1994 and 2004. the clinical signs included ataxia, indications of anosmia, blindness, and abnormal behavior. the disease was characterized by severe necrotizing encephalitis affecting mostly the cranial cerebrum, basal ganglia, and olfactory bulbs. investigations to identify the etiology of the disease included tes ... | 2007 | 17459845 |
| the onset of rapidly progressive neurologic deterioration after a brief gastrointestinal illness in a renal allograft recipient. | renal transplant recipients are at increased risk for life-threatening complications, most commonly infections. because of their impaired cell-mediated immunity, these patients are particularly susceptible to organisms that rely on intracellular survival and spread, such as listeria monocytogenes. despite being a food-borne pathogen, l. monocytogenes is associated with significant morbidity and mortality. here we report the case of a renal transplant recipient who developed rapidly progressive n ... | 2007 | 17462001 |
| application of real-time quantitative pcr for the detection of selected bacterial pathogens during municipal wastewater treatment. | bacteria were detected at five stages of municipal wastewater treatment using taqman(r) real-time quantitative pcr (qpcr). thirteen probe and primer sets were tested for diverse pathogens that may be present in wastewater, including aeromonas hydrophila, bacillus cereus, clostridium perfringens, enterococcus faecalis, escherichia coli, e. coli o157:h7, helicobacter pylori, klebsiella pneumoniae, legionella pneumophila, listeria monocytogenes, pseudomonas aeruginosa, salmonella sp., and staphyloc ... | 2007 | 17462712 |
| canine cathelicidin (k9cath): gene cloning, expression, and biochemical activity of a novel pro-myeloid antimicrobial peptide. | cathelicidins, a group of cationic peptides found in leukocytes and epithelial cells, play a central role in the early innate immune defense against infection. although these host defense peptides have been reported in several mammalian species, including primates, no cathelicidins have been identified in carnivores. here we report the cloning, tissue expression and biological activity of a novel canine cathelicidin (k9cath). the full-length cdna sequence of k9cath encodes a predicted 172 amino ... | 2007 | 17462733 |
| development of matrix lysis for concentration of gram positive bacteria from food and blood. | the development of a fast, reliable and inexpensive protocol for the concentration of bacteria from food by the removal of fat, carbohydrates and proteins that is compatible with downstream alternative dna-based quantification methods is described. the protocol was used for dairy products, cooked and smoked fish and meat, carbohydrate-rich cooked products, ready-to-eat sauces, egg and blood. lysis resulted in pellets of reasonable size for further processing. starch, plant materials, fungi, tiss ... | 2007 | 17462766 |
| temperature-assisted pressure inactivation of listeria monocytogenes in turkey breast meat. | ready-to-eat turkey breast meat samples were surface-inoculated with a five-strain cocktail of listeria monocytogenes cultures to a final concentration of approximately 10(7) cfu/g. the inoculated meat samples were vacuum-packaged and pressure treated at 300 mpa for 2 min, 400 mpa for 1 min, and 500 mpa for 1 min at initial sample temperatures of 1, 10, 20, 30, 40, 50, and 55 degrees c. l. monocytogenes was most resistant to pressure at temperatures between 10 and 30 degrees c. as temperature de ... | 2007 | 17462773 |
| identification of new genes associated with intermediate resistance of enterococcus faecalis to divercin v41, a pediocin-like bacteriocin. | it has been suggested that resistance to class iia bacteriocins occurs at either a low or a high level. in listerial strains, low-level resistance (2-4-fold) to class iia bacteriocins is attributed to alterations in membrane lipid composition. in listeria monocytogenes and enterococcus faecalis, high-level resistance (1000-fold) correlates with inactivation of the mptacd operon, which encodes the eii(man)(t) mannose permease of the phosphotransferase system (pts). previous studies reported that ... | 2007 | 17464076 |
| hygienic quality of ewes' milk cheeses manufactured with artisan-produced lamb rennet pastes. | the use of artisan-produced lamb rennet pastes, but not any of the other commercial animal rennets, imparts a characteristic flavour to the cheese, so most mediterranean ewes' milk cheeses are coagulated with this kind of rennet paste. in contrast to the advantages of using lamb or kid rennet pastes from the sensory point of view, questions are still raised as to their hygienic quality. the goal was to examine the microbiological and enzymic quality of lamb rennet pastes prepared by cheese manuf ... | 2007 | 17466112 |
| innate immune activation of cd4 t cells in salmonella-infected mice is dependent on il-18. | production of ifn-gamma by cd4 t cells is generally thought to be mediated by tcr triggering, however, ag-nonspecific activation of effector cd8 t cells has been reported in infection models. in this study, we demonstrate that ag-experienced cd4 t cells in the spleen of salmonella-infected mice acquire the capacity to rapidly secrete ifn-gamma in response to stimulation with bacterial lysate or lps. this innate responsiveness of t cells was transient and most apparent during, and immediately fol ... | 2007 | 17475863 |
| evaluation of the international reference methods nf en iso 11290-1 and 11290-2 and an in-house method for the isolation of listeria monocytogenes from retail seafood products in france. | retail seafood products were analyzed on their use-by date using the international reference methods nf en iso 11290-1 and 11290-2 (collectively method r) or an in-house method (method b) for the isolation of listeria monocytogenes. the sensitivity of the methods was about 78%. method r detected more positive samples of smoked salmon and herb-flavored slices of smoked salmon than did method b, whereas the reverse was true for samples of carpaccio-like salmon, herb-flavored slices of raw salmon, ... | 2007 | 17477258 |
| antimicrobial effects of alginate-based films containing essential oils on listeria monocytogenes and salmonella typhimurium present in bologna and ham. | bologna and ham slices (300 of each) were inoculated with salmonella typhimurium or listeria monocytogenes at 10(3) cfu/cm(2). alginate-based edible films that had been immersed in a 2 or 20% (wt/vol) cac12 solution and contained 1% (wt/ vol) essential oils of spanish oregano (o; corydothymus capitatus), chinese cinnamon (c; cinnamomum cassia), or winter savory (s; satureja montana) were then applied to slices to control pathogen growth. on bologna, c-based films pretreated with 20% cac12 were t ... | 2007 | 17477259 |
| microbiological safety of retail vacuum-packed and modified-atmosphere-packed cooked meats at end of shelf life. | a study of retail modified-atmosphere-packed and vacuum-packed cooked ready-to-eat meats was undertaken from september through mid-november 2003 to determine the microbiological quality at the end of shelf life and to establish any risk factors in the production, storage, and display of this product. examination of 2,981 samples using microbiological guidelines criteria revealed that 66% were of satisfactory or acceptable microbiology quality, 33% were of unsatisfactory quality mainly due to hig ... | 2007 | 17477265 |
| development of thermal surrogate microorganisms in ground beef for in-plant critical control point validation studies. | in search of a suitable surrogate microorganism for in-plant critical control point validation, we compared the rates of thermal inactivation of three bacteria, enterococcus faecium b2354, pediococcus parvulus hp, and pediococcus acidilactici lp, to those of listeria monocytogenes and salmonella. ground beef samples containing 4 and 12% fat were inoculated with e. faecium, l. monocytogenes, and salmonella senftenberg 775w and heated at 58, 62, 65, or 68 degrees c. the decimal reduction times (d- ... | 2007 | 17477266 |
| inhibition of listeria monocytogenes using nisin with grape seed extract on turkey frankfurters stored at 4 and 10 degrees c. | recontamination of cooked ready-to-eat (rte) chicken and beef products with listeria monocytogenes has been a major safety concern. natural antimicrobials in combinations can be an alternative approach for controlling l. monocytogenes. therefore, the objectives of this study were to evaluate the inhibitory activities against l. monocytogenes of nisin (6,400 iu/ ml), grape seed extract (gse; 1%), and the combination of nisin and gse both in tryptic soy broth with 0.6% yeast extract (tsbye) and on ... | 2007 | 17477277 |
| evaluation in vitro of the antagonistic substances produced by lactobacillus spp. isolated from chickens. | to determine the inhibitory capacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. isolates identified as l. reuteri, l. salivarius, or lactobacillus spp. inhibited enteroco ... | 2007 | 17479773 |
| soluble metals in residual oil fly ash alter innate and adaptive pulmonary immune responses to bacterial infection in rats. | the soluble metals of the pollutant, residual oil fly ash (rofa), have been shown to alter pulmonary bacterial clearance in rats. the goal of this study was to determine the potential effects on both the innate and adaptive lung immune responses after bacterial infection in rats pre-exposed to the soluble metals in rofa. sprague-dawley rats were intratracheally dosed (i.t.) at day 0 with rofa (r-total) (1.0 mg/100 g body weight), the soluble fraction of rofa (r-soluble), the soluble sample subje ... | 2007 | 17481688 |
| listeria monocytogenes fecal shedding in dairy cattle shows high levels of day-to-day variation and includes outbreaks and sporadic cases of shedding of specific l. monocytogenes subtypes. | fecal shedding of listeria monocytogenes poses a risk for contamination of animal feed and agricultural environments and raw food at the pre-harvest stages of food production. to be able to reduce these risks it is critical to improve understanding of the epidemiology of l. monocytogenes shedding in feces. the objective of this study was to assess the daily variability of fecal shedding and its association with individual animal (lactation number and the day of current lactation) and environment ... | 2007 | 17481754 |
| listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. | the contribution of bacterial infection to tumorigenesis is usually ascribed to infection-associated inflammation. an alternate view is that direct interaction of bacteria with tumor cells promotes tumor progression. here, we show that the microenvironment of large tumors favors bacterial survival, which in turn directly accelerates tumor growth by activating tumor cell toll-like receptors (tlr). listeria monocytogenes (lm) survives in the microenvironment of large but not small tumors, resultin ... | 2007 | 17483348 |
| modification of chicken avian beta-defensin-8 at positively selected amino acid sites enhances specific antimicrobial activity. | antimicrobial peptides (amps), essential components of innate immunity, are found in a range of phylogenetically diverse species and are thought to act by disrupting the membrane integrity of microbes. in this paper, we used evolutionary signatures to identify sites that are most relevant during the functional evolution of these molecules and introduced amino acid substitutions to improve activity. we first demonstrate that the anti-microbial activity of chicken avian beta-defensin-8, previously ... | 2007 | 17483936 |
| a kinematic description of the trajectories of listeria monocytogenes propelled by actin comet tails. | the bacterial pathogen listeria monocytogenes propels itself in the cytoplasm of the infected cells by forming a filamentous comet tail assembled by the polymerization of the cytoskeletal protein actin. although a great deal is known about the molecular processes that lead to actin-based movement, most macroscale aspects of motion, including the nature of the trajectories traced out by the motile bacteria, are not well understood. here, we present 2d trajectories of listeria moving between a gla ... | 2007 | 17485664 |
| the 2-cys peroxiredoxin-deficient listeria monocytogenes displays impaired growth and survival in the presence of hydrogen peroxide in vitro but not in mouse organs. | resistance of listeria monocytogenes to reactive oxygen radicals may facilitate its survival in phagocytic cells and against some oxidizing sanitizers. the aim of this study was to investigate the function of the 2-cys peroxiredoxin (prx) homologue in l. monocytogenes, particularly its survival in a hydrogen peroxide-containing environment. an in-frame prx deletion mutant and a complementation strain were constructed and evaluated for their growth and survival either in media containing differen ... | 2007 | 17486404 |
| vapor-phase activities of cinnamon, thyme, and oregano essential oils and key constituents against foodborne microorganisms. | the aim of the study presented here was to gain knowledge about the vapor-phase antimicrobial activity of selected essential oils and their major putatively active constituents against a range of foodborne bacterial and fungal strains. in a first step, the vapor-phase antimicrobial activities of three commercially available essential oils (eos)-cinnamon (cinnamomum zeylanicum), thyme (thymus vulgaris), and oregano (origanum vulgare)-were evaluated against a wide range of microorganisms, includin ... | 2007 | 17488023 |
| rapid and sensitive magnetoelastic biosensors for the detection of salmonella typhimurium in a mixed microbial population. | in this article, we report the results of an investigation into the performance of a wireless, magnetoelastic biosensor designed to selectively detect salmonella typhimurium in a mixed microbial population. the langmuir-blodgett (lb) monolayer technique was employed for antibody (specific to salmonella sp.) immobilization on rectangular shaped strip magnetoelastic sensors (2 x 0.4 x 0.015 mm). bacterial binding to the antibody on the sensor surface changes the resonance parameters, and these cha ... | 2007 | 17490768 |
| comparative in vitro antimicrobial activity of tigecycline, a new glycylcycline compound, in freshly prepared medium and quality control. | the in vitro spectra of activity of tigecycline and tetracycline were determined for 2,490 bacterial isolates representing 50 different species or phenotypic groups. all isolates were tested simultaneously by broth microdilution using freshly prepared mueller-hinton broth and by disk diffusion. portions of these data were submitted to the food and drug administration (fda) in support of the sponsor's application for new drug approval. in a separate study, mic and disk diffusion quality control r ... | 2007 | 17494717 |
| plasma-mediated grafting of poly(ethylene glycol) on polyamide and polyester surfaces and evaluation of antifouling ability of modified substrates. | a simple cold plasma technique was developed to functionalize the surfaces of polyamide (pa) and polyester (pet) for the grafting of polyethylene glycol (peg) with the aim of reducing biofilm formation. the surfaces of pa and pet were treated with silicon tetrachloride (sicl4) plasma, and peg was grafted onto plasma-functionalized substrates (pa-peg, pet-peg). different molecular weights of peg and grafting times were tested to obtain optimal surface coverage by peg as monitored by electron spec ... | 2007 | 17500575 |
| interactions between circadian rhythm and immunity in drosophila melanogaster. | | 2007 | 17502084 |
| thrombospondin-1 promotes cellular adherence of gram-positive pathogens via recognition of peptidoglycan. | thrombospondin-1 (tsp1) is a matricellular glycoprotein that has key roles in interactions between human cells and components of the extracellular matrix. here we report a novel role for the lectin tsp1 in pathogen-host interactions. binding assays and flow cytometric analysis demonstrate that streptococcus pneumoniae and other gram-positive pathogens including s. pyogenes, staphylococcus aureus, and listeria monocytogenes interact specifically with human tsp1. we also show for the first time th ... | 2007 | 17507668 |
| herpesvirus latency confers symbiotic protection from bacterial infection. | all humans become infected with multiple herpesviruses during childhood. after clearance of acute infection, herpesviruses enter a dormant state known as latency. latency persists for the life of the host and is presumed to be parasitic, as it leaves the individual at risk for subsequent viral reactivation and disease. here we show that herpesvirus latency also confers a surprising benefit to the host. mice latently infected with either murine gammaherpesvirus 68 or murine cytomegalovirus, which ... | 2007 | 17507983 |
| listeria monocytogenes encephalitis mimicking herpes simplex virus encephalitis: the differential diagnostic importance of cerebrospinal fluid lactic acid levels. | listeria monocytogenes is a common cause of bacterial meningitis in elderly patients and in those with impaired cellular immunity. the most common central nervous system infection caused by l. monocytogenes is acute bacterial meningitis; meningoencephalitis is uncommon and encephalitis is rare. early diagnosis of l. monocytogenes meningitis is difficult because only 50% of cerebrospinal fluid (csf) gram stains are negative. l. monocytogenes is one of the few central nervous system pathogens asso ... | 2007 | 17509430 |
| lactoferrin from canine neutrophils: isolation and physicochemical and antimicrobial properties. | lactoferrin has been isolated from canine leukocytes for the first time. lactoferrin was identified by n-terminal amino acid sequence and by capability to capture ferric cations resulting in a complex with absorbance maximum at 460-470 nm. it is demonstrated that canine lactoferrin resembles the human homolog in some physicochemical properties, i.e. molecular weight, carbohydrate presence, and conditions of protein-iron complex dissociation. bactericidal activity of dog lactoferrin was demonstra ... | 2007 | 17511610 |
| spr biosensor for the detection of l. monocytogenes using phage-displayed antibody. | whole cells of listeria monocytogenes were detected with a compact, surface plasmon resonance (spr) sensor using a phage-displayed scfv antibody to the virulence factor actin polymerization protein (acta) for biorecognition. phage lm p4:a8, expressing the scfv antibody fused to the piii surface protein was immobilized to the sensor surface through physical adsorption. a locally constructed fluidics system was used to deliver solutions to the compact, two-channel spreeta sensor. specificity of th ... | 2007 | 17512186 |
| influence of treatment time and pulse frequency on salmonella enteritidis, escherichia coli and listeria monocytogenes populations inoculated in melon and watermelon juices treated by pulsed electric fields. | consumption of unpasteurized melon and watermelon juices has caused several disease outbreaks by pathogenic microorganisms worldwide. pulsed electric field (pef) has been recognized as a technology that may inactivate those bacteria present in fluid food products at low temperatures. hence, pef treatment at 35 kv/cm, 4 mus pulse duration in bipolar mode and square shape were applied on salmonella enteritidis, e. coli and l. monocytogenes populations inoculated in melon and watermelon juices with ... | 2007 | 17512997 |
| a virus-like particle-based vaccine selectively targeting soluble tnf-alpha protects from arthritis without inducing reactivation of latent tuberculosis. | neutralization of the proinflammatory cytokine tnf-alpha by mabs or soluble receptors represents an effective treatment for chronic inflammatory disorders such as rheumatoid arthritis, psoriasis, or crohn's disease. in this study, we describe a novel active immunization approach against tnf-alpha, which results in the induction of high titers of therapeutically active autoantibodies. immunization of mice with virus-like particles of the bacteriophage qbeta covalently linked to either the entire ... | 2007 | 17513796 |
| biophysical modeling of forward scattering from bacterial colonies using scalar diffraction theory. | a model for forward scattering from bacterial colonies is presented. the colonies of interest consist of approximately 10(12) - 10(13) individual bacteria densely packed in a configuration several millimeters in diameter and approximately 0.1-0.2 mm in thickness. the model is based on scalar diffraction theory and accounts for amplitude and phase modulation created by three macroscopic properties of the colonies: phase modulation due to the surface topography, phase modulation due to the radial ... | 2007 | 17514326 |
| cytolysin-dependent escape of the bacterium from the phagosome is required but not sufficient for induction of the th1 immune response against listeria monocytogenes infection: distinct role of listeriolysin o determined by cytolysin gene replacement. | listeria monocytogenes evades the antimicrobial mechanisms of macrophages by escaping from the phagosome into the cytosolic space via a unique cytolysin that targets the phagosomal membrane, listeriolysin o (llo), encoded by hly. gamma interferon (ifn-gamma), which is known to play a pivotal role in the induction of th1-dependent protective immunity in mice, appears to be produced, depending on the bacterial virulence factor. to determine whether the llo molecule (the major virulence factor of l ... | 2007 | 17517863 |
| [strategies for the development of new tuberculosis vaccines]. | tuberculosis remains a substantial global health problem causing 2 million deaths, and an estimated 8 to 10 million new infections a year. the efficacy of the mycobacterium bovis bacillus calmette-guérin (bcg), the only available antituberculosis vaccine, is variable (0-80%), especially in tuberculosis-endemic countries. over the past decade there has been a resurgence of interest in the development of new tuberculosis vaccines and some of the most promising are now entering into early clinical ... | 2007 | 17519853 |
| a plastic microchip for nucleic acid purification. | a microchip for purifying nucleic acids from bacterial pathogens was designed and fabricated in plastic. the fabricated plastic microchips were tested for their ability to purify nucleic acids from the bacteria listeria monocytogenes (l. monocytogenes), escherichia coli (e. coli), and salmonella typhimurium (s. typhimurium). these chips were constructed using rapid and low-cost plastic fabrication techniques including hot embossing and plastic casting. silicon molds fabricated by photolithograph ... | 2007 | 17530410 |
| a rapid multiplexed chemiluminescent immunoassay for the detection of escherichia coli o157:h7, yersinia enterocolitica, salmonella typhimurium, and listeria monocytogenes pathogen bacteria. | a simple and rapid multiplexed sandwich chemiluminescent enzyme immunoassay has been developed for the simultaneous detection of escherichia coli o157:h7, yersinia enterocolitica, salmonella typhimurium, and listeria monocytogenes. to achieve the multiplexed detection of the four pathogens, a new polystyrene 96 well microtiter plate format has been designed, in which each main well contains four subwells in the bottom. the monoclonal antibodies specific for each bacteria were separately immobili ... | 2007 | 17530767 |
| characterization of a broad range antibacterial substance from a new bacillus species isolated from amazon basin. | a bacillus sp. strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. the phylogenetic analysis indicated that this strain has low sequence similarity with most bacillus spp., suggesting a new species was isolated. the antimicrobial activity was detected starting at the exponential growth phase, and maximum activity was observed at stationary phase. the substance was inhibitory to a broad range of indicator strains, including pathogenic ... | 2007 | 17534601 |
| application of automated ribotyping to support the evaluation of listeria monocytogenes sources in a taleggio cheese producing plant. | in march 2005, listeria monocytogenes was detected on the rinds of taleggio cheeses produced in an italian plant. to identify the pathogen source, 154 rinds of cheeses that had been manually and automatically salinated and 52 environmental swabs collected from salting equipment, ripening cloths, and ripening boxes were tested for l. monocytogenes. twenty-seven strains isolated from cheese samples and 16 strains isolated from environmental samples were genotyped by ecori and pvuii automated ribot ... | 2007 | 17536669 |
| dose-response of listeria monocytogenes after oral exposure in pregnant guinea pigs. | listeriosis, a severe disease that results from exposure to the foodborne pathogen listeria monocytogenes, is responsible for approximately 2500 illnesses and 500 deaths in the united states each year. pregnant women are 20 times more likely to develop listeriosis than the general population, with adverse pregnancy outcomes that include spontaneous abortions, stillbirths, and neonatal meningitis. the objective of this study was to determine an infective dose that resulted in stillbirths and infe ... | 2007 | 17536670 |
| proteomic analysis of a hypochlorous acid-tolerant listeria monocytogenes cultural variant exhibiting enhanced biofilm production. | following exposure of listeria monocytogenes scott a (sa) to hypochlorous acid, rough colony variants were identified that were tolerant of hypochlorous acid and produced increased amounts of biofilm. a derivative of one of these variants was smooth, produced even more biofilm, and exhibited greater biofilm chlorine resistance. the objective of this research was to compare the protein expression of a cultural variant to sa and to identify proteins that might be associated with biofilm production ... | 2007 | 17536671 |
| extracting additional risk managers information from a risk assessment of listeria monocytogenes in deli meats. | the risk assessment study of listeria monocytogenes in ready-to-eat foods conducted by the u.s. food and drug administration is an example of an extensive quantitative microbiological risk assessment that could be used by risk analysts and other scientists to obtain information and by managers and stakeholders to make decisions on food safety management. the present study was conducted to investigate how detailed sensitivity analysis can be used by assessors to extract more information on risk f ... | 2007 | 17536672 |
| antimicrobial activity of olive oil, vinegar, and various beverages against foodborne pathogens. | the survival of foodborne pathogens in aqueous extracts of olive oil, virgin olive oil, vinegar, and several beverages was evaluated. vinegar and aqueous extracts of virgin olive oil showed the strongest bactericidal activity against all strains tested. red and white wines also killed most strains after 5 min of contact, black and green tea extracts showed weak antimicrobial activity under these conditions, and no effect was observed for the remaining beverages (fruit juices, coca-cola, dairy pr ... | 2007 | 17536679 |
| inhibitory activity of colicin e1 against listeria monocytogenes. | colicins are gram-negative bacteriocins produced by and effective against escherichia coli and related species. colicin e1 (cole1) is composed of three functional domains, which collectively have a pore-forming effect on targeted bacteria. cole1 binding and translocation domains are highly specific in contrast to the pore-forming domain, implying that cole1 could be broadly effective. in this study, the activity of cole1 against listeria monocytogenes was evaluated in broth and on surfaces of re ... | 2007 | 17536690 |
| use of one-ply composite tissues in an automated optical assay for recovery of listeria from food contact surfaces and poultry-processing environments. | a novel one-ply composite tissue (ct) method using the soleris (formerly biosys) optical analysis system was compared with the conventional u.s. department of agriculture (usda) environmental sponge enrichment method for recovery of listeria from food contact surfaces and poultry-processing environments. stainless steel and high-density polyethylene plates were inoculated to contain a six-strain l. monocytogenes cocktail at 10(4), 10(2), and 10 cfu per plate, whereas samples from naturally conta ... | 2007 | 17536691 |
| effectiveness and stability of plastic films coated with nisin for inhibition of listeria monocytogenes. | plastic films were coated with a cellulose-based carrier solution containing nisin, a natural antimicrobial peptide with the potential to inhibit growth of food spoilage and pathogenic microorganisms such as listeria monocytogenes. five commercial plastic films with different chemical compositions and surface properties were compared in this study: low-density polyethylene, ethylene-vinyl acetate copolymer, and three types of ethylene-methacrylic acid copolymers: surlyn 1601, nucrel 0403, and nu ... | 2007 | 17536692 |
| minimally processed vegetable salads: microbial quality evaluation. | the increasing demand for fresh fruits and vegetables and for convenience foods is causing an expansion of the market share for minimally processed vegetables. among the more common pathogenic microorganisms that can be transmitted to humans by these products are listeria monocytogenes, escherichia coli o157:h7, and salmonella. the aim of this study was to evaluate the microbial quality of a selection of minimally processed vegetables. a total of 181 samples of minimally processed leafy salads w ... | 2007 | 17536694 |
| an overview of microbial food safety programs in beef, pork, and poultry from farm to processing in canada. | canada's vision for the agri-food industry in the 21st century is the establishment of a national food safety system employing hazard analysis and critical control point (haccp) principles and microbiological verification tools, with traceability throughout the gate-to-plate continuum. voluntary on-farm food safety (offs) programs, based in part on haccp principles, provide producers with guidelines for good production practices focused on general hygiene and biosecurity. offs programs in beef c ... | 2007 | 17536696 |
| impact of selected lactobacillus and bifidobacterium species on listeria monocytogenes infection and the mucosal immune response. | the pathogenesis of listeria monocytogenes depends on its ability to attach to and invade the gastrointestinal epithelium and subsequently withstand the host immune response. despite a thorough understanding of the intracellular phase of infection, relatively little is known about how the pathogen behaves in the gastrointestinal tract and whether it is affected by the presence of host commensal microbiota. lactobacillus and bifidobacterium are two important genera of the human gut microbiota pro ... | 2007 | 17537177 |
| a predictive model for the influence of food components on survival of listeria monocytogenes lm 54004 under high hydrostatic pressure and mild heat conditions. | the combination of high hydrostatic pressure with mild temperature was explored to achieve a predictive model of microbial inactivation in food matrix processing. the pressure processing conditions were fixed at 448 mpa for 11 min at the treatment temperature of 41 degrees c, which have been determined as the optimum processing conditions considering six log-cycle reductions of listeria monocytogenes. based on the results, response surface methodology (rsm) was performed in the present work, the ... | 2007 | 17537535 |
| avirulent viable but non culturable cells of listeria monocytogenes need the presence of an embryo to be recovered in egg yolk and regain virulence after recovery. | the aim of this study was to assess the efficiency of the embryonated egg model to recover viable but non culturable (vbnc) cells of listeria monocytogenes. l. monocytogenes cells were incubated in filtered sterilised distilled water. the vbnc state was obtained after a 25 to 47 days incubation period (concentration of culturable cells less than 1 cfu/ml). fifteen days after the vbnc state was reached, non culturability was checked in various media. one milliliter of each vbnc suspension that co ... | 2007 | 17540159 |
| extending the host range of listeria monocytogenes by rational protein design. | in causing disease, pathogens outmaneuver host defenses through a dedicated arsenal of virulence determinants that specifically bind or modify individual host molecules. this dedication limits the intruder to a defined range of hosts. newly emerging diseases mostly involve existing pathogens whose arsenal has been altered to allow them to infect previously inaccessible hosts. we have emulated this chance occurrence by extending the host range accessible to the human pathogen listeria monocytogen ... | 2007 | 17540170 |
| synthesis, characterization and antimicrobial activity of new aliphatic sulfonamide. | a series of novel aliphatic sulfonamide derivatives (1-7) were synthesized and characterized by elemental analyses, ft-ir, (1)h nmr, (13)c nmr and lc-ms techniques. all the synthesized compounds were evaluated in vitro as antimicrobial agents against representative strains of gram-positive (staphylococcus aureus atcc 25953, bacillus cereus atcc 6633 and listeria monocytogenes atcc li6 (isolate), gram-negative bacteria (escherichia coli atcc 11230) and antifungal agent against candida albicans (c ... | 2007 | 17544281 |
| rates of processing determine the immunogenicity of immunoproteasome-generated epitopes. | cd8 t cells resolve intracellular pathogens by responding to pathogen-derived peptides that are presented on the cell surface by mhc class i molecules. although most pathogens encode a large variety of antigenic peptides, protective cd8 t cell responses target usually only a few of these. to determine the mechanism by which the ifn-gamma-inducible proteasome (immuno) subunits enhance the ability of specific pathogen-derived peptides to elicit cd8 t cell responses, we generated a recombinant list ... | 2007 | 17548590 |
| allelic penetrance approach as a tool to model two-locus interaction in complex binary traits. | many binary phenotypes do not follow a classical mendelian inheritance pattern. interaction between genetic and environmental factors is thought to contribute to the incomplete penetrance phenomena often observed in these complex binary traits. several two-locus models for penetrance have been proposed to aid the genetic dissection of binary traits. such models assume linear genetic effects of both loci in different mathematical scales of penetrance, resembling the analytical framework of quanti ... | 2007 | 17551528 |
| [epidemiology of listeriosis in germany: a changing but ignored pattern]. | listeriosis is emerging as a food-borne disease. although the incidence of clinical presentations is low, this infectious disease with a rather poor prognosis deserves attention. approximately 20-30% of patients with recognized listeriosis die in spite of an appropriate antibiotic regimen. in the last two years the number of registered cases of listeriosis in germany has more than doubled. this increase is clearly due exclusively to the rising number of infections in elderly persons, while the n ... | 2007 | 17551889 |
| [mode of action of plantaricin l-1, an antilisteria bacteriocin produced by lactobacillus plantarum]. | plantaricin l-1, an anti-listeria bacteriocin, was produced by lactobacillus plantarum and successfully purified by sp-sepharose ff cation exchange chromatography. the mechanism on energized cells of listeria monocytogenes was studied with purified plantaricin l-1. after adding plantaricin l-1 to listeria monocytogenes at 64 au/ml, leakage of intercellular k+ ions, inorganic phosphate, lactic dehydrogenase, uv-absorbing materials and the intracellular atp was observed, and the action resulted in ... | 2007 | 17552231 |
| culture-positive sepsis in neonatal camelids: 21 cases. | there is limited literature on neonatal bacterial sepsis in new world (nw) camelids. | 2007 | 17552461 |