| a rapid elisa-based method for screening bordetella pertussis strain production of antigens included in current acellular pertussis vaccines. | despite extensive vaccinations, there have been pertussis epidemics in many countries including the netherlands, the uk, australia and the usa. during these epidemics bordetella pertussis strains not producing the vaccine antigen pertactin (prn) are emerging and increasing in numbers. however, methods for confirming prn production of b. pertussis isolates are combined pcr or pcr-based sequencing tests and western blotting. furthermore, data about production of pertussis toxin (pt) and filamentou ... | 2014 | 24925807 |
| an engineered 3d human airway mucosa model based on an sis scaffold. | to investigate interrelations of human obligate airway pathogens, such as bordetella pertussis, and their hosts test systems with high in vitro/in vivo correlation are of urgent need. using a tissue engineering approach, we generated a 3d test system of the airway mucosa with human tracheobronchial epithelial cells (htec) and fibroblasts seeded on a clinically implemented biological scaffold. to investigate if htec display tumour-specific characteristics we analysed raman spectra of htec and the ... | 2014 | 24912816 |
| estimating the effectiveness of tetanus-diphtheria-acellular pertussis vaccine (tdap) for preventing pertussis: evidence of rapidly waning immunity and difference in effectiveness by tdap brand. | we estimated the vaccine effectiveness (ve) of tetanus-diphtheria-acellular pertussis vaccine (tdap) for preventing pertussis among adolescents during a statewide outbreak of pertussis in wisconsin during 2012. | 2014 | 24903664 |
| development of an animal model to evaluate the allergenicity of food allergens. | considering the increasing numbers of patients suffering from food allergy (fa) as well as the great variety of novel foods and food compositions, an unmet need exists for the development of preclinical approaches to characterize the allergenic potential of proteins. the aim of our study was to evaluate the allergenicity of different food allergens in a rat model. | 2014 | 24903216 |
| prevalence of antibody to bordetella pertussis in neonates and prevalence of recent pertussis infection in pregnant women in catalonia (spain) in 2003 and 2013. | infections because of bordetella pertussis still occur in infants and adults in european countries, despite vaccination coverage against pertussis being high. | 2014 | 24871642 |
| temperature-accelerated molecular dynamics gives insights into globular conformations sampled in the free state of the ac catalytic domain. | the catalytic domain of the adenyl cyclase (ac) toxin from bordetella pertussis is activated by interaction with calmodulin (cam), resulting in camp overproduction in the infected cell. in the x-ray crystallographic structure of the complex between ac and the c terminal lobe of cam, the toxin displays a markedly elongated shape. as for the structure of the isolated protein, experimental results support the hypothesis that more globular conformations are sampled, but information at atomic resolut ... | 2014 | 24863163 |
| surveillance of antimicrobial resistance in contemporary clinical isolates of bordetella pertussis in ontario, canada. | | 2014 | 24837412 |
| high prevalence of erythromycin-resistant bordetella pertussis in xi'an, china. | resistance of bordetella pertussis, the causative agent of pertussis, to erythromycin is rare. recently, several chinese isolates were found to be erythromycin resistant. this study aimed to investigate the occurrence of pertussis in children suffering persistent cough and the prevalence of b. pertussis resistance to erythromycin in xi'an, china. three hundred and thirteen patients with suspected pertussis admitted to xi'an children's hospital from january 2012 through to december 2013 were incl ... | 2014 | 24816168 |
| human dendritic cell maturation and cytokine secretion upon stimulation with bordetella pertussis filamentous haemagglutinin. | in addition to antibodies, th1-type t cell responses are also important for long-lasting protection against pertussis. however, upon immunization with the current acellular vaccines, many children fail to induce th1-type responses, potentially due to immunomodulatory effects of some vaccine antigens, such as filamentous haemagglutinin (fha). we therefore analysed the ability of fha to modulate immune functions of human monocyte-derived dendritic cells (mddc). fha was purified from pertussis toxi ... | 2014 | 24801497 |
| b-cell responses after intranasal vaccination with the novel attenuated bordetella pertussis vaccine strain bpze1 in a randomized phase i clinical trial. | despite high vaccination coverage, pertussis is still a global concern in infant morbidity and mortality, and improved pertussis vaccines are needed. a live attenuated bordetella pertussis strain, named bpze1, was designed as an intranasal vaccine candidate and has recently been tested in man in a phase i clinical trial. here, we report the evaluation of the b-cell responses after vaccination with bpze1. forty-eight healthy males with no previous pertussis-vaccination were randomized into one of ... | 2014 | 24793938 |
| modeling rates of infection with transient maternal antibodies and waning active immunity: application to bordetella pertussis in sweden. | serological surveys provide reliable information from which to calculate forces (instantaneous rates) of infection, but waning immunity and clinical consequences that depend on residual immunity complicate interpretation of results. we devised a means of calculating these rates that accounts for passively acquired maternal antibodies that decay or active immunity that wanes, permitting re-infection. we applied our method to pertussis (whooping cough) in sweden, where vaccination was discontinued ... | 2014 | 24768867 |
| neonatal extracorporeal membrane oxygenation: initial experience of hospital de são joão. | the purpose of this series is to report the initial ecmo experience of the neonatal intensive care unit of hospital de são joão. the first three clinical cases are reported. case report 1: a 39 weeks gestational age girl with severe lung hypoplasia secondary to a bilateral congenital diaphragmatic hernia. case report 2: a 39 weeks gestational age girl with a right congenital diaphragmatic hernia and a tracheal stenosis. case report 3: a 34 weeks gestational age boy, with 61 days of life, with a ... | 2014 | 24768509 |
| [immunobiological properties of bordetella pertussis lipopolysaccharide in the acellular pertussis vaccine]. | study of bordetella pertussis lipopolysaccharide (lps) immunobiological properties in the acellular pertussis vaccine. | 2014 | 24738289 |
| seroprevalence of bordetella pertussis antibody in pregnant women in iran. | the increasing incidence of pertussis among adolescents and adults in recent years is an alarming factor in transmission of the infection to non-immune infants and children. vaccination of pregnant women, immediately after delivery and before being discharged from the hospital may help to protect mothers and their newborns against the disease. decision making process, regarding maternal immunization, requires credible information and knowledge about seroepidemiology of the infection in pregnant ... | 2014 | 24728747 |
| healthcare providers as sources of vaccine-preventable diseases. | vaccine-preventable infectious diseases may be introduced into the healthcare setting and pose a serious risk to vulnerable populations including immunocompromised patients. healthcare providers (hcps) are exposed to these pathogens through their daily tasks and may serve as a reservoir for ongoing disease transmission in the healthcare setting. the primary method of protection from work-related infection risk is vaccination that protects not only an individual hcp from disease, but also subsequ ... | 2014 | 24726251 |
| immune response to bordetella pertussis is associated with season and undernutrition in senegalese children. | while vaccines elicit a protective response in most recipients, studies suggest that environmental and nutritional factors can influence the strength of the individual response to immunization and to subsequent natural infectious challenges. | 2014 | 24726248 |
| epidemiology of pertussis in children of flanders belgium: can healthcare professionals be involved in the infection? | pertussis is a communicable disease whose registration incidence in the flemish region of belgium has increased since 2003. originally, this increment was obvious only in the province of antwerp, but since 2012, there has also been an increase in the registered cases of pertussis in other flemish provinces. the overall registration incidence for pertussis in flanders was 5·6 per 100 000 in 2012. the majority of these cases were identified among young children, but older children and adults were ... | 2014 | 24724749 |
| conformational dynamics of protein transporter fhac: large-scale motions of plug helix. | fhac is an integral outer membrane protein of the whooping cough agent bordetella pertussis that mediates the transport to the cell surface of a major virulence factor, the filamentous haemagglutinin adhesin fha. the fha/fhac pair is a prototypic tpsa/tpsb system of the widespread 'two-partner secretion' pathway, dedicated to the transport of long extracellular proteins in various pathogenic and environmental gram-negative bacteria. fhac belongs to the ubiquitous omp85 superfamily of protein tra ... | 2014 | 24646315 |
| recommended vaccines for adolescent patients. | vaccination is a safe and effective tool in the prevention of serious and deadly infectious diseases. the vaccinations delivered at routine adolescent visits are an integral part of preventing infection, as well as reducing school absenteeism as a result of illness. current recommendations by the advisory committee on immunization practices include vaccinating during a single visit at age 11 or 12 years against pathogens including neisseria meningitidis, which has been shown to cause invasive me ... | 2014 | 24636971 |
| vaccinated children and adolescents with pertussis infections experience reduced illness severity and duration, oregon, 2010-2012. | bordetella pertussis causes severe respiratory illness among infants and adolescents. high proportions of breakthrough infection have been observed. to understand the effect of vaccination in the era of acellular pertussis vaccines (dtap and tdap), we assessed if vaccination status is associated with disease severity and duration. | 2014 | 24633685 |
| mouse and pig models for studies of natural and vaccine-induced immunity to bordetella pertussis. | the increasing incidence of whooping cough in many developed countries has been linked with waning immunity induced after immunization with acellular pertussis (ap) vaccines. the rational design of an improved ap vaccine requires a full understanding of the mechanism of protective immunity and preclinical studies in animal models. infection of mice and pigs with bordetella pertussis has many features of the infection seen in humans and has already provided valuable information on the roles of in ... | 2014 | 24626866 |
| can we conquer coqueluche? | | 2014 | 24626865 |
| changing pertussis epidemiology: everything old is new again. | before vaccination, pertussis was a universal disease of early childhood. although apparent control of the disease in the united states and other countries was achieved through vaccination, pertussis is resurgent. though acellular vaccines have been in use for 20 years, new data are emerging on their effectiveness and durability of protection and the contribution of these characteristics to the resurgence of pertussis. | 2014 | 24626532 |
| [preclinical studies of an adsorbed diphtheria-tetanus-pertussis vaccine (adtp-vaccine) with acellular pertussis component]. | evaluate standardness of antigenic composition of pertussis component, completeness of sorption of pertussis, diphtheria and tetanus components, specific activity and safety of experimental series ofadtp-vaccine with acellular pertussis component (adtap-vaccine). | 2014 | 24605674 |
| healthcare-associated pertussis outbreak in arizona: challenges and economic impact, 2011. | an outbreak investigation identified 15 pertussis cases among 5 infants and 10 healthcare professionals at 1 hospital's neonatal intensive care unit (nicu). the cost of the outbreak to this hospital was $97 745. heightened awareness of pertussis in nicus is key to preventing healthcare-associated spread and minimizing outbreak-control-related costs. bordetella pertussis is a highly communicable bacterial pathogen that causes a prolonged cough illness and is spread by respiratory droplet transmis ... | 2014 | 26624909 |
| absence of antibodies against bordetella pertussis in pregnant women and newborns in the state of nuevo leon. | to assess placental transfer of antibodies to the child at birth and at 2 months of age. | 2014 | 24572974 |
| human bocavirus respiratory infection in infants in córdoba, argentina. | it has been suggested that human bocavirus (hbov) is related to acute respiratory infection (ari) in children (prevalence: 0.9% to 33%) although clinical characteristics have not been clearly established yet. | 2014 | 24566786 |
| epidemiology of bordetella pertussis in a children's hospital. | pertussis or whooping cough continues to be a major cause of morbidity and mortality in infants younger than 1 year old. | 2014 | 24566778 |
| antibody-mediated inhibition of bordetella pertussis adenylate cyclase-haemolysin-induced macrophage cytotoxicity is influenced by variations in the bacterial population. | whooping cough is a vaccine-preventable disease presenting with epidemic cycles linked to natural and/or vaccine-driven evolution of the aetiological agent of the disease, bordetella pertussis. adenylate cyclase-haemolysin (ac-hly) is a major toxin produced by this pathogen, which mediates macrophage apoptosis in vitro and in vivo. while current acellular pertussis vaccine (apv) formulations do not include ac-hly, they all contain pertussis toxin and can comprise filamentous haemagglutinin (fha) ... | 2014 | 24554758 |
| prevalence of bordetella infection in a hospital setting in niamey, niger. | bordetella pertussis still poses an important health threat in developing countries. in niger, notified pertussis cases are few despite the low diphtheria-tetanus-pertussis/pentavalent vaccine coverage. we aimed to estimate the prevalence of b. pertussis in children aged <5 years consulting at a pediatric ward. a 5-month study in 2011 recruited 342 children with respiratory symptoms at the national hospital of niamey. nasopharyngeal aspirates were tested by culture and real-time polymerase chain ... | 2014 | 24531376 |
| do we need a new vaccine to control the re-emergence of pertussis? | bordetella pertussis causes whooping cough and is re-emerging in developed countries despite widespread immunization with acellular pertussis vaccines (pa), which are less effective than the whole cell vaccines that they replaced. efficacy of pa could be improved by switching from alum to alternative adjuvants that generate more potent cell mediated immunity. | 2014 | 24485284 |
| peripheral smear clues for bordetella pertussis. | | 2013 | 24479133 |
| efficacy of face masks and respirators in preventing upper respiratory tract bacterial colonization and co-infection in hospital healthcare workers. | we compared the efficacy of medical masks (mm) and n95 respirators (n95) in preventing bacterial colonization/infection in healthcare workers (hcws). | 2014 | 24472436 |
| consistency of bordetella pertussis vaccine seed strains and potency of whole-cell pertussis vaccine still in use in poland. | in poland, where the wp vaccine has been used since 1960, pertussis rates increased in the mid-1990s. in 2012, the rate of pertussis recognised by surveillance was unexpectedly found to be two-fold higher than in the previous decade. quality measures on potency and vaccine working seeds were introduced, to confirm the possible impact of manufacturing inconsistency or potency lowering on the observed increase in pertussis. shewhart charts on potency values for lots released between 2001 and 2013 ... | 2014 | 24457195 |
| [reproducibility of fim2 and fim3 antigens determination in bordetella pertussis by serotyping method]. | serotyping is a commonly used method to characterize the presence of fimbriae 2 and 3 in bordetella pertussis strains for epidemiological purposes and optimal choice of strain composition of the pertussis whole-cell vaccine. monoclonal antisera against fim2 and fim3 are recommended to be used for microplate serotyping instead ofpolyclonal. reliable evaluation offimbriae expressed by b. pertussis strains influence interpretation of vaccine-driven strain evolution. | 2013 | 24432556 |
| [detection of bordetella within the framework of the eupert-labnet bordetella pcr eqa]. | the aim of this study was evaluation of molecular identification results of samples including genomic dna of bordetella by using pcr method, obtained by laboratory of department of bacteriology nizp-pzh and their comparison with the results obtained by other reference laboratories in eu. the study was conducted within the framework of the first external quality assessment (eupert-labnet bordetella pcr eqa). | 2013 | 24432555 |
| prospective nationwide surveillance of hospitalizations due to pertussis in children, 2006-2010. | frequency of pertussis is highly variable from country to country and it depends on multiple factors including case definitions and type of surveillance systems used. many countries recently reported an increase of pertussis cases especially in infants and adolescents. | 2014 | 24413406 |
| quantification of potassium levels in cells treated with bordetella adenylate cyclase toxin. | the aim of this study was to compare two methods for quantification of changes in intracellular potassium concentration (decrease from ∼140 to ∼20mm) due to the action of a pore-forming toxin, the adenylate cyclase toxin (cyaa) from the pathogenic bacterium bordetella pertussis. cyaa was incubated with stably transfected k1 chinese hamster ovary cells expressing the toxin receptor cd11b/cd18 and the decrease in potassium concentration in the cells was followed by inductively coupled plasma mass ... | 2014 | 24412166 |
| acellular pertussis vaccine based on outer membrane vesicles capable of conferring both long-lasting immunity and protection against different strain genotypes. | despite high vaccination coverage rates, pertussis continues to be a global concern, with increased incidence widely noted. the current pertussis epidemiologic situation has been mainly attributed to waning immunity and pathogen adaptation. to improve the disease control, a new generation of vaccines capable to overcome those weaknesses associated to the current vaccines need to be developed. previously we have demonstrated that the outer membrane vesicles obtained from the recombinant bordetell ... | 2014 | 24397896 |
| intention to accept bordetella pertussis booster vaccine during pregnancy in mexico city. | adult booster vaccination against pertussis can help prevent severe infections in young infants. we examined influences on intention to accept pertussis booster vaccination among pregnant women in mexico city. | 2014 | 24394441 |
| usefulness of laboratory methods in diagnosis of pertussis in adult with paroxysmal cough. | pertussis is an acute, highly contagious bacterial infection of respiratory system caused by bordetella pertussis. principally, disease affects young children, however, recently it is also reported in adolescents and adults. symptoms of pertussis in adults are non-specific, i.e. dry, paroxysmal and protracted cough. thus, it is rarely diagnosed in this group. | 2014 | 25848782 |
| [evaluation of bordetella pertussis strains toxicity in vitro using cho cell lines]. | whooping cough is still a significant disease with regular outbreaks despite the decades of mass vaccination and good immunization coverage. the aim of this study was to evaluate the capacity of bordetella pertussis toxicity testing among strains harbouring different alleles of the pertussis toxin promoter ptxp using hamster ovary cell line cho (hamster ovary). | 2014 | 25804072 |
| pertactin-negative variants of bordetella pertussis in new york state: a retrospective analysis, 2004-2013. | the first report of pertactin-negative variants of bordetella pertussis in the united states has raised questions about the role of acellular pertussis vaccines in the recent increase of pertussis cases. our laboratory utilized a sequence-based method to identify mutations in the pertactin gene responsible for these variants and assessed vaccination status from the associated cases. | 2014 | 24365130 |
| the relationship between concentration of specific antibody at birth and subsequent response to primary immunization. | trans-placentally acquired antibodies can protect infants from infection in the first months of life. however, high concentrations of antibody at birth may impact the infant's own immune response to primary immunization. we examine the relationship between concentration of specific antibody to bordetella pertussis, haemophilus influenzae type b (hib), tetanus toxoid and pneumococcal antigens at birth and following primary immunization. | 2014 | 24342250 |
| [experimental whooping cough of nonhuman primate]. | despite considerable success in study of bordetella pertussis virulence factors, pathogenesis of whooping cough, duration of b. pertussis bacteria persistence, types and mechanisms of immune response are still keep underinvestigated. it can be explained by the absence ofadequate experimental animal model for pertussis study. our study estimates clinical and laboratory parameters of whooping cough in non-human primates of the old world in the process of intranasan infection by virulent b. pertuss ... | 2013 | 24340642 |
| vaccines for children and adults with chronic lung disease: efficacy against acute exacerbations. | acute exacerbations of chronic lung disease are usually associated with viral and bacterial pathogens. they contribute to declining lung function, poor quality of life and exert an excess burden on individuals, families, communities and the healthcare sector. hence, preventing exacerbations is important in clinical management. several vaccines providing protection against respiratory pathogens (streptococcus pneumoniae, bordetella pertussis and influenza) that can trigger exacerbations are avail ... | 2014 | 24308654 |
| clustered cases of bordetella pertussis infection cause high levels of igg antibodies against pertussis toxin in adolescents in gaobeidian city, china. | summary in a large serosurvey performed in gaobeidian city, china, 1032 children and adolescents aged 3-18 years were divided into three age groups: 3-6, 7-11, and 12-18 years. the geometric mean of igg antibodies against pertussis toxin (anti-pt igg) varied from 3.47 iu/ml (3-6 years) to 8.71 iu/ml (12-18 years) and the seroprevalence (≥ 40 iu/ml) varied from 4.48% (7-11 years) to 11.76% (12-18 years). infections were clustered in three secondary schools (schools 1-3). however, excluding second ... | 2014 | 24308586 |
| time since last vaccine dose in pcr-positive and pcr-negative children with suspected pertussis to monitor pertussis vaccine effectiveness. | we evaluated whether the results of diagnostic polymerase chain reaction (pcr) testing combined with time since last vaccine dose could be used to monitor the effectiveness of acellular pertussis vaccines. in 258 consecutive nasopharyngeal swabs from children and adolescents with typical pertussis symptoms, 80 were positive and 178 were negative in pcr for bordetella pertussis dna (is 481). time since last vaccine dose was available for 152 patients, of which 120 were fully immunised. among the ... | 2014 | 24292098 |
| pertussis in the central-west region of brazil: one decade study. | in many parts of the world, numerous outbreaks of pertussis have been described despite high vaccination coverage. in this article we report the epidemiological characteristics of pertussis in brazil using a surveillance worksheet. secondary data of pertussis case investigations reported from january 1999 to december 2008 recorded in the information system for notifiable diseases (sinan) and the central laboratory for public health (lacen-ms) were utilized. the total of 561 suspected cases were ... | 2014 | 24275370 |
| pertussis. | pertussis, a disease that has been well described since the middle ages, has a worldwide distribution and can infect all ages. it is caused by the gram-negative, pleomorphic bacillus bordetella pertussis, which is transmitted from human to human via aerosolized droplets at close range. descriptions such as the one-hundred day cough in chinese and whooping cough in english, describe the severity of this disease seen in both the developed and the developing world. | 2013 | 24227570 |
| epidemiology of bordetella pertussis in san luis potosí, mexico. | we analyzed data from 147 patients with suspected pertussis in san luis potosí, mexico. bordetella pertussis was detected by real-time polymerase chain reaction in 59 (40.1%) cases. the incidence of b. pertussis infection was 2.3 per 100,000 population. there were 6 deaths among the study patients. we conclude that the impact of pertussis in our state is significantly higher than previously estimated. | 2014 | 24220229 |
| whooping cough: identification, assessment and management. | although there is a high uptake of vaccinations providing protection against bordetella pertussis, the main cause of whooping cough, there has been an increase in the incidence of notifications of the disease in the uk and other developed countries in recent years. the increase in cases of whooping cough is mainly evident in older children and adults. while these individuals may experience persistent and unpleasant symptoms, most notably prolonged cough, symptoms may be mild, in part, because mo ... | 2016 | 24219484 |
| pertussis epidemic despite high levels of vaccination coverage with acellular pertussis vaccine. | we describe the pertussis epidemic, based only on confirmed whooping cough cases. we have analyzed data on the diagnosis, epidemiology and vaccine history in order to understand the factors that might explain the trends of the disease. | 2015 | 24216286 |
| different t cell memory in preadolescents after whole-cell or acellular pertussis vaccination. | to better understand vaccine-induced protection and its potential failure in light of recent whooping cough resurgence, we evaluated quantity as well as quality of memory t cell responses in b. pertussis-vaccinated preadolescent children. using a technique based on flow cytometry to detect proliferation, cytokine production and phenotype of antigen-specific cells, we evaluated residual t cell memory in a cohort of preadolescents who received a whole-cell pertussis (wp; n=11) or an acellular pert ... | 2013 | 24176499 |
| interchangeability of quinvaxem during primary vaccination schedules: results from a phase iv, single-blind, randomized, controlled, single-center, non-inferiority study. | combination vaccines against diphtheria, tetanus and pertussis (dtp) represent the core of childhood vaccination programs. quinvaxem, a fully-liquid, pentavalent combination vaccine containing inactivated hepatitis b (hepb), haemophilus influenzae type b (hib) and whole-cell pertussis (wp) antigens, and tetanus and diphtheria toxoids, has been shown to be suitable for boosting children primed in infancy with another dtwp-hepb-hib vaccine. this single-blind, randomized, controlled study was desig ... | 2014 | 24176498 |
| protective role of adenylate cyclase in the context of a live pertussis vaccine candidate. | despite high vaccination coverage, pertussis remains an important respiratory infectious disease and the least-controlled vaccine-preventable infectious disease in children. natural infection with bordetella pertussis is known to induce strong and long-lasting immunity that wanes later than vaccine-mediated immunity. therefore, a live attenuated b. pertussis vaccine, named bpze1, has been developed and has recently completed a phase i clinical trial in adult human volunteers. in this study, we i ... | 2014 | 24140230 |
| [clinical research of bordetella pertussis infection in infants with prolonged cough]. | to explore the prevalence of bordetella pertussis (b. pertussis) infection in unvaccinated or incomplete vaccinated infants with cough for a prolonged duration. | 2013 | 24124679 |
| bordetella pertussis: why is it still circulating? | bordetella pertussis is the causal agent of whooping cough, a highly contagious respiratory disease that is life-threatening in infants under the age of three months and may also be very severe in pregnant women and seniors. this disease can be prevented by vaccination but it remains a public health problem in many developed and developing countries.(1) so, why is b. pertussis still circulating? we need to consider several aspects of this vaccine-preventable disease when answering this question: ... | 2014 | 24103807 |
| evaluation of several approaches to immunize parents of neonates against b. pertussis. | parental immunization ("cocooning") is a potentially effective strategy to protect neonates against bordetella pertussis. the objective of this study was to evaluate three approaches to parental immunization: (1) current practice (single dtap dose to adolescents, one additional dose recommended in adults); (2) promotion of vaccination in the maternity ward, with vaccine offered in the community; and (3) promotion and administration of vaccine in the maternity ward. | 2013 | 24099874 |
| bordetella pertussis seroprevalence in belgian adults aged 20-39 years, 2012. | summary the last report on pertussis seroprevalence in belgium concerned samples collected during 1993-1994. in the context of the eupert-labnet wp6 seroprevalence study (comparing sera from 16 european member states), 1500 anonymized leftover diagnostic samples were collected randomly during the second semester of 2012 by the clinical chemistry laboratories of six participating belgian centres, distributed equally between flanders, wallonia and brussels capital region. as suggested by the wp6 o ... | 2014 | 24073712 |
| [pertussis predictors in hospitalized infants with acute lower respiratory tract infection]. | pertussis, or whooping cough, caused by bordetella pertussis (bp) is a re-emerging problem in our environment. although generally considered that the disease is relatively easy to identify infections respiratory syncytial virus (rsv) can present with similar symptoms in infants remains difficult discrimination. | 2013 | 24067589 |
| pertussis vaccines: state-of-the-art and future trends. | bordetella pertussis is the causative agent of whooping cough (pertussis) which is a worldwide vaccine preventable acute respiratory illness that predominantly involves infants. the reactogenicity of whole-cell (pw) vaccines and the difficulty of their consistent production have led to the development of acellular pertussis (pa) vaccines. however, despite high vaccination coverage using either pw or pa and introduction of adolescent and adult vaccines with reduced antigen content, there are stil ... | 2013 | 24066885 |
| prospective nationwide surveillance of hospitalizations due to pertussis in children, 2006-2010. | frequency of pertussis is highly variable from country to country and it depends on multiple factors including case definitions and type of surveillance systems used. many countries recently reported an increase of pertussis cases especially in infants and adolescents. | 2013 | 24064564 |
| identification of biomarkers to detect residual pertussis toxin using microarray analysis of dendritic cells. | in this study we aimed to identify genes that are responsive to pertussis toxin (ptx) and might eventually be used as biological markers in a testing strategy to detect residual ptx in vaccines. by microarray analysis we screened six human cell types (bronchial epithelial cell line beas-2b, fetal lung fibroblast cell line mrc-5, primary cardiac microvascular endothelial cells, primary pulmonary artery smooth muscle cells, hybrid cell line ea.hy926 of umbilical vein endothelial cells and epitheli ... | 2013 | 24055089 |
| [clinical and epidemiological differences between bordetella pertussis and respiratory syncytial virus infections in infants: a matched case control study]. | an increase in cases of pertussis, mainly in young infants, has been reported in the last few years. the clinical presentation of this disease is very similar to that produced by respiratory syncytial virus (rsv), which makes the diagnosis difficult. | 2014 | 24054972 |
| pertussis in poland in 2011. | results of the nationwide pertussis epidemiology study implemented by department of epidemiology niph-nih in 2009-2011 showed a high proportion of cases of whooping cough in adults whose only symptom of bordetella pertussis infection was long lasting cough. it should be noted that the infected adults pose a risk of transmission of infection to susceptible contacts such as infants. | 2013 | 24040716 |
| respiratory viral infections in infants with clinically suspected pertussis. | to evaluate the frequency of respiratory viral infections in hospitalized infants with clinical suspicion of pertussis, and to analyze their characteristics at hospital admission and clinical outcomes. | 2013 | 24035869 |
| experience with monocomponent acellular pertussis combination vaccines for infants, children, adolescents and adults--a review of safety, immunogenicity, efficacy and effectiveness studies and 15 years of field experience. | combination vaccines containing a monocomponent acellular pertussis (ap) vaccine, manufactured at statens serum institut (ssi), denmark, have successfully controlled bordetella pertussis infections in denmark since 1997. the efficacy of this ap vaccine was 71% in a double-blind, randomised and controlled clinical trial. its safety and immunogenicity have been demonstrated in infants, children, adolescents and adults. in approximately 500,000 children it was effective against pertussis requiring ... | 2013 | 23994021 |
| respiratory infection promotes t cell infiltration and amyloid-β deposition in app/ps1 mice. | alzheimer's disease (ad) is a progressive neurodegenerative disease characterized by deposits of amyloid-β and neurofibrillary tangles. it has been suggested that inflammatory changes are associated with disease; however, it has not been established whether these are a consequence of ongoing neurodegeneration or whether inflammation itself contributes to disease pathogenesis. recent studies suggest that exposure to infection can accelerate cognitive decline in ad patients, and pathogens have bee ... | 2014 | 23993702 |
| [seroepidemiology of pertussis in 10-15 years old healthy children in isparta province, turkey]. | pertussis, a highly contagious respiratory disease, commonly affects infants and young children and can be fatal, especially in babies less than one year of age. bordetella pertussis continues to circulate even in populations where a high vaccine coverage of infants and children is achieved. adults are reservoirs for infections in infants, in whom pertussis may be severe and life-threatening. despite the rising rates of vaccination in our country and all over the world for the past 25 years, the ... | 2013 | 23971928 |
| severe infantile bordetella pertussis pneumonia in monozygotic twins with a congenital c3 deficiency. | bordetella pertussis or whooping cough is a vaccine-preventable disease that still remains a serious infection in neonates and young infants. we describe two young infants, monozygotic twins, with a severe b. pertussis pneumonia of whom one needed extracorporeal membrane oxygenation. diagnostic work-up of unexplained hematuria and proteinuria during the illness revealed low serum complement component 3 (c3) levels. during follow-up, c3 levels remained low (400-600 mg/l). extensive analysis of th ... | 2014 | 23963626 |
| the virulence factors of bordetella pertussis: talented modulators of host immune response. | approximately 40 million whooping cough cases and between 200,000 and 400,000 pertussis-linked deaths are recorded each year. although several types of vaccines are licensed and widely used, bordetella pertussis continues to circulate in populations with high vaccine coverage of infants and children due to the waning of protection induced by the vaccination. b. pertussis typically expresses a wide array of virulence factors which promote bacterial adhesion and invasion by altering the local envi ... | 2013 | 23955529 |
| peptide sharing between bordetella pertussis proteome and human sudden death proteins: a hypothesis for a causal link. | to explore the molecular basis of the relationship between bordetella pertussis infection and sudden death. | 2013 | 23902149 |
| bordetella pertussis entry into respiratory epithelial cells and intracellular survival. | bordetella pertussis is the causative agent of pertussis, aka whooping cough. although generally considered an extracellular pathogen, this bacterium has been found inside respiratory epithelial cells, which might represent a survival strategy inside the host. relatively little is known, however, about the mechanism of internalization and the fate of b. pertussis inside the epithelia. we show here that b. pertussis is able to enter those cells by a mechanism dependent on microtubule assembly, li ... | 2013 | 23893966 |
| toll-like receptor 4 orchestrates neutrophil recruitment into airways during the first hours of bordetella pertussis infection. | most of the knowledge on the impact of bordetella pertussis lipo-oligosaccharide (los) on the infectious process was obtained when the bacteria was established within the host. the aim of the present work was to determine the role of tlr4 at a very early step of the infectious process. to this end we used a transcriptomic approach on b. pertussis intranasal infection model in c3h/hen, a tlr4-competent mouse strain, and c3h/hej, a tlr4-deficient mouse strain. the expression of approximately 140 g ... | 2016 | 23811096 |
| pertussis. | pertussis, or whopping cough, is an upper respiratory tract infection caused by bordetella pertussis. it has long been a concern in pediatric populations, leading to aggressive vaccination strategies to help decrease pediatric disease. in recent years, recognition of pertussis infection in adult populations has increased, leading to more frequent diagnosis and recommendations for booster immunizations in the adult population. early recognition and treatment as well as vaccination will help reduc ... | 2013 | 23809713 |
| mannose receptor and macrophage galactose-type lectin are involved in bordetella pertussis mast cell interaction. | mast cells are crucial in the development of immunity against bordetella pertussis, and the function of tlrs in this process has been investigated. here, the interaction between mast cells and b. pertussis with an emphasis on the role of clrs is examined. in this study, two clrs, mgl and mr, were detected for the first time on the surface of mast cells. the involvement of mr and mgl in the stimulation of mast cells by heat-inactivated bp was investigated by the use of blocking antibodies and spe ... | 2013 | 23794711 |
| [hooping cough in adults, think about it!]. | we report the case of a 47 year old patient who had been suffering from persistent cough for more than three weeks. patient coughed predominantly during night time, without fever. the amoxicillin-clavulanic acid initially prescribed was not effective. a series of complementary investigations were performed before serology finally identified bordetella pertussis infection after two months of symptoms which improved slowly without evident benefit of macrolide treatment. the diagnosis of whooping c ... | 2013 | 23755707 |
| a novel method for evaluating natural and vaccine induced serological responses to bordetella pertussis antigens. | we studied the time course of serum igg antibodies against 3 different pertussis vaccine antigens: pt (pertussis toxin), fha (filamentous hemagglutinin), prn (pertactin) in sera from individuals vaccinated with four different pertussis vaccines at 4 years of age: (n=44, 44, 23 and 23, respectively,) and compared the responses to/after natural infection with bordetella pertussis (n=44, age 1-8 years). these longitudinal data were analyzed with a novel method, using a mathematical model to describ ... | 2013 | 23742995 |
| seroprevalence of bordetella pertussis in the mexican population: a cross-sectional study. | summary serum samples collected during the national health and nutrition survey (ensanut 2006) were obtained from subjects aged 1-95 years (january-october 2010) and analysed to assess the seroprevalence of bordetella pertussis (bp) in mexico. subjects' gender, age, geographical region and socioeconomic status were extracted from the survey and compiled into a subset database. a total of 3344 subjects (median age 29 years, range 1-95 years) were included in the analysis. overall, bp seroprevalen ... | 2014 | 23734968 |
| comparison of lipopolysaccharide structures of bordetella pertussis clinical isolates from pre- and post-vaccine era. | endotoxins are lipopolysaccharides (lps), and major constituents of the outer membrane of gram-negative bacteria. bordetella pertussis lps were the only major antigens, of this agent of whooping-cough, that were not yet analyzed on isolates from the pre- and post-vaccination era. we compared here the lps structures of four clinical isolates with that of the vaccine strain bp 1414. all physico-chemical analyses, including sds-page, tlc, and different maldi mass spectrometry approaches were conver ... | 2013 | 23731797 |
| bordetella pertussis iron regulated proteins as potential vaccine components. | bordetella pertussis is the etiologic agent of whooping cough, an illness whose incidence has been increasing over the last decades. pertussis reemergence despite high vaccination coverage, together with the recent isolation of circulating strains deficient in some of the vaccine antigens, highlight the need for new vaccines. proteins induced under physiological conditions, such as those required for nutrient acquisition during infection, might represent good targets for better preventive strate ... | 2013 | 23731630 |
| [pathophysiology, diagnostics and therapy of chronic cough: neuronal reflexes and antitussiva]. | cough is the number one symptom for patients to visit a physician worldwide. it is an important neuronal reflex which serves to protect the airways from inhaled exogenous microorganisms, thermal and chemical irritants. moreover, it prevents the airways from mucus retention.the cough reflex is initiated by activation of different cough receptors. these cough receptors can be divided into three groups according to their electrophysiological properties: into the two aδ-fiber types "rapid-adapting m ... | 2013 | 23700135 |
| role of (p)ppgpp in biofilm formation and expression of filamentous structures in bordetella pertussis. | bordetella pertussis, the causative agent of whooping cough, is highly adapted to cause human infection. the production of virulence factors, such as adhesins and toxins, is just part of an array of mechanisms by which b. pertussis causes infection. the stringent response is a global bacterial response to nutritional limitation that is mediated by the accumulation of cellular ppgpp and pppgpp [termed together as (p)ppgpp]. here, we demonstrate that production of (p)ppgpp was controlled by rela a ... | 2013 | 23676431 |
| qualitative assessment of pertussis diagnostics in united states laboratories. | united states national surveillance data show that the use of culture for pertussis diagnostics has sharply declined, whereas polymerase chain reaction (pcr) is now the most common testing method. pcr testing for pertussis is rapid and sensitive, but the lack of standardization and variable specificity is concerning. | 2013 | 23587980 |
| pneumococcal surface protein a does not affect the immune responses to a combined diphtheria tetanus and pertussis vaccine in mice. | the pneumococcal surface protein a (pspa) is a promising candidate for the composition of a protein vaccine against streptococcus pneumoniae. we have previously shown that the whole cell bordetella pertussis vaccine (wp) is a good adjuvant to pspa, inducing protective responses against pneumococcal infection in mice. in brazil, wp is administered to children, formulated with diphtheria and tetanus toxoids (dtpw) and aluminum hydroxide (alum) as adjuvant. a single subcutaneous dose of pspa5-dtplo ... | 2013 | 23541622 |
| bordetella pertussis infection in infants: a reemerging disease. | bordetella pertussis is a highly contagious bacterial disease currently on the rise in the united states. the most vulnerable age group is infants younger than 1 year old. the reasons for the current outbreak are multifactorial. the following is a case report describing a recent case of bordetella pertussis infection admitted to a neonatal intensive care unit. | 2013 | 23532029 |
| cd137 ligand signaling enhances myelopoiesis during infections. | cd137 and its ligand are expressed in the bm, and conflicting data exist on the regulation of myelopoiesis by the cd137 receptor-ligand system. cd137(-/-) mice have increased numbers of myeloid cells in the bm, indicating an inhibitory influence of cd137 on myelopoiesis. however, cd137 also induces proliferation of hematopoietic progenitor cells and their myeloid differentiation, arguing for an enhancing effect. here we hypothesized that this latter case represents the situation during infection ... | 2013 | 23519951 |
| vaccinal status of healthcare students in lille. | vaccine recommendations are the same for healthcare students than for other health care workers. the aim of this study was to describe mandatory and recommended vaccinal coverage and evaluate the benefit of systematic monitoring by a medical team in the lille medical school. | 2013 | 23498136 |
| alveolar macrophages contribute to respiratory tolerance by inducing foxp3 expression in naive t cells. | alveolar macrophages (ams) from mice and humans have long been known to contribute to maintaining tolerance in the lung. studies have shown that ams can induce anergy in cd4(+) t cells. nitric oxide, prostaglandins, and leukotrienes have been implicated in am-mediated tolerance. however, it remains unclear what effect, if any, ams exert on foxp3 induction in cd4(+) t cells from mice and humans, and whether or not other immunomodulators might play a role. ams were isolated from bronchoalveolar la ... | 2013 | 23492186 |
| high-resolution melting analysis for the detection of two erythromycin-resistant bordetella pertussis strains carried by healthy schoolchildren in china. | two erythromycin-resistant strains of bordetella pertussis were isolated from nasopharyngeal specimens of two asymptomatic schoolchildren in china. high-resolution melting and sequencing analyses confirmed the homogeneous a2047g mutation in 23s rrna genes of the two isolates. high-resolution melting (hrm) analysis is a useful assay for the rapid detection of erythromycin-resistant b. pertussis. the appearance of erythromycin-resistant b. pertussis strains in china is alarming. | 2013 | 23480481 |
| characteristics of severe bordetella pertussis infection among infants ≤90 days of age admitted to pediatric intensive care units - southern california, september 2009-june 2011. | bordetella pertussis infection can cause severe illness and death among young infants. | 2013 | 26619437 |
| the protective role of maternally derived antibodies against bordetella pertussis in young infants. | in 20 infants with polymerase chain reaction-confirmed pertussis <6 months of age, median cord blood anti-pertussis toxin, anti-filamentous hemagglutinin and anti-pertactin igg antibody values were lower than in 80 matched controls: 10.5 versus 13.5 anti-pertussis toxin iu/ml, 14.5/18.0 (anti-filamentous hemagglutinin) and 6.0/9.0 (anti-pertactin), respectively. although differences of median (and mean) antibody values between groups were not significant, they are in line with the concept of inf ... | 2013 | 23429559 |
| bordetella pertussis infection attenuates clinical course of acute bronchiolitis. | bordetella pertussis (bp) was detected in hospitalized children with various lower respiratory tract infections. the aim of this study was to assess clinical characteristics of bp infection, and its effect on disease severity in young children hospitalized with acute bronchiolitis. | 2013 | 23429556 |
| persistence at one year of age of antigen-induced cellular immune responses in preterm infants vaccinated against whooping cough: comparison of three different vaccines and effect of a booster dose. | due to their high risk of developing severe bordetella pertussis (bp) infections, it is recommended to immunize preterm infants at their chronological age. however, little is known about the persistence of their specific immune responses, especially of the cellular responses recognized to play a role in protection. we compared here the cellular immune responses to two major antigens of bp between three groups of one year-old children born prematurely, who received for their primary vaccination r ... | 2013 | 23429006 |
| evaluation of the filmarray® respiratory panel for clinical use in a large children's hospital. | respiratory pathogens are a leading cause of hospital admission and traditional detection methods are time consuming and insensitive. multiplex molecular detection methods have recently been investigated in hope of replacing these traditional techniques with rapid panel-based testing. | 2013 | 23424157 |
| epidemiological and immunological reasons for pertussis vaccination in adolescents and adults. | the resurgence of pertussis has been the subject of considerable debate. hypotheses to explain increased reporting in developed countries have focused mainly on three aspects: (1) increased recognition of the disease in adolescents and adults; (2) waning of vaccine-induced immunity and (3) loss of vaccine efficacy due to an antigenic shift of bordetella pertussis. waning immunity after vaccination or natural infection combined with the absence of regular boosters either in the form of vaccine bo ... | 2013 | 23419520 |
| fasciola hepatica tegumental coat impairs mast cells' ability to drive th1 immune responses. | the parasitic worm fasciola hepatica induces strong th2 and t-regulatory immune responses while simultaneously suppressing th1-driven immune responses to bystander microbial infections. it also prevents the initiation of th1-mediated autoimmune disorders in mice through the suppression of th17 and th1 immune responses, and this can be mimicked by parasite-derived molecules. we have isolated f. hepatica tegumental coat ag (fhteg) and demonstrated its suppressive effect in vivo by directly targeti ... | 2013 | 23418624 |
| pertussis resurgence: waning immunity and pathogen adaptation - two sides of the same coin. | pertussis or whooping cough has persisted and resurged in the face of vaccination and has become one of the most prevalent vaccine-preventable diseases in western countries. the high circulation rate of bordetella pertussis poses a threat to infants that have not been (completely) vaccinated and for whom pertussis is a severe, life-threatening, disease. the increase in pertussis is mainly found in age groups in which immunity has waned and this has resulted in the perception that waning immunity ... | 2014 | 23406868 |
| respiratory tract infections during the 2011 mycoplasma pneumoniae epidemic. | in 2011, norway experienced a surge in community acquired mycoplasma pneumoniae infections. norway also has one of the highest rates of reported bordetella pertussis infections, despite high vaccine coverage. we aimed to determine the prevalence of upper respiratory tract pathogens in patients attending primary care physicians for respiratory illness during the 2011 m. pneumoniae epidemic period. a retrospective analysis of data from 26,039 patients that have had nasopharyngeal swabs analysed by ... | 2013 | 23354674 |