| estimated and reported incidence of pertussis in estonian adults: a seroepidemiological study. | rates of pertussis immunisation among children in estonia are high (∼95%), but pertussis is still the most common vaccine preventable childhood disease. adults are suspected to be sources of pertussis in children. we aimed to measure pertussis toxin (pt) igg in adults to estimate pertussis infection activity and compare estimated and reported pertussis incidences. | 2015 | 26277068 |
| frequency and characteristics of hospital-sponsored pertussis revaccination programs in the southern united states. | the centers for disease control and prevention (cdc) released guidelines in december, 2006, recommending revaccination against bordetella pertussis with tdap for all post-partum women and healthcare workers. the cdc recommendations specifically state "the postpartum tdap should be administered before discharge from the hospital or birthing center" and "hospitals and ambulatory-care facilities should provide tdap for healthcare personnel." the purpose of this survey was to determine the frequency ... | 2015 | 26272891 |
| t-cell immune responses to bordetella pertussis infection and vaccination. | the recent immunological investigations, stemming from the studies performed in the nineties within the clinical trials of the acellular pertussis vaccines, have highlighted the important role played by t-cell immunity to pertussis in humans. these studies largely confirmed earlier investigations in the murine respiratory infection models that humoral immunity alone is not sufficient to confer protection against bordetella pertussis infection and that t-cell immunity is required. over the last y ... | 2015 | 26242279 |
| seroprevalence studies of pertussis: what have we learned from different immunized populations. | bordetella pertussis is a pathogen-causing whooping cough (pertussis) in humans. although vaccination against the disease is effective, the bacterium is still circulating among population and can even cause death. especially young infants, who lack protection, are vulnerable. the laboratory diagnostic methods to detect b. pertussis are culture, pcr and elisa serology. reported cases of the disease vary among countries but usually the incidence rates are low, <1 to 10/100 000. however, pertussis ... | 2015 | 26208655 |
| the potential of adjuvants to improve immune responses against tdap vaccines: a preclinical evaluation of mf59 and monophosphoryl lipid a. | the successful approach of combining diphtheria, tetanus and pertussis antigens into a single vaccine has become a cornerstone of immunization programs. yet, even if vaccination coverage is high, a resurgence of pertussis has been reported in many countries suggesting current vaccines may not provide adequate protection. to induce better tailored and more durable immune responses against pertussis vaccines different approaches have been proposed, including the use of novel adjuvants. licensed ap ... | 2015 | 26149936 |
| bisamidate prodrugs of 2-substituted 9-[2-(phosphonomethoxy)ethyl]adenine (pmea, adefovir) as selective inhibitors of adenylate cyclase toxin from bordetella pertussis. | novel small-molecule agents to treat bordetella pertussis infections are highly desirable, as pertussis (whooping cough) remains a serious health threat worldwide. in this study, a series of 2-substituted derivatives of 9-[2-(phosphonomethoxy)ethyl]adenine (pmea, adefovir), in their isopropyl ester bis(l-phenylalanine) prodrug form, were designed and synthesized as potent inhibitors of adenylate cyclase toxin (act) isolated from b. pertussis. the series consists of pmea analogues bearing either ... | 2015 | 26136378 |
| detection of antibodies against fimbria type 3 (fim3) is useful diagnostic assay for pertussis. | isolation of bordetella pertussis and detection of the pertussis genome are not always successful because of low bacterial loads in adult patients with pertussis. antibodies against pertussis toxin (pt) are measured but have low sensitivity in vaccinated subjects. there is no reliable diagnostic method at present. in this study, a fluorescent-eia against several pertussis antigens and genome detection were investigated to establish clinical laboratory diagnostic methods for pertussis. the study ... | 2015 | 26134278 |
| respiratory infection with a bacterial pathogen attenuates cns autoimmunity through il-10 induction. | infection with viral or bacterial pathogens has been linked with the development of multiple sclerosis (ms), while infection with helminth parasites has been associated protection against ms and other autoimmune diseases. here we have used a murine model of ms, experimental autoimmune encephalomyelitis (eae), to examine the effect of infection with the respiratory pathogen bordetella pertussis infection on development of cns inflammation. the data demonstrate that infection of mice with b. pertu ... | 2015 | 26100487 |
| needle-free and adjuvant-free epicutaneous boosting of pertussis immunity: preclinical proof of concept. | the limited durability of pertussis vaccine-induced protection requires novel approaches to reactivate immunity and limit pertussis resurgence in older children and adults. we propose that periodic boosters could be delivered using a novel epicutaneous delivery system (viaskin) to deliver optimized pertussis antigens such as genetically-detoxified pertussis toxin (rpt). to best mimic the human situation in which vaccine-induced memory cells persist, whereas antibodies wane, we developed a novel ... | 2015 | 26067183 |
| a field study of household attack rates and the effectiveness of macrolide antibiotics in reducing household transmission of pertussis. | bordetella pertussis (whooping cough) is an endemic, highly contagious bacterial respiratory infection, which is notifiable to australian state and territory health departments. between 2008 and 2011 there was a substantial outbreak in new south wales with an initial increase in cases occurring in north coast new south wales from late 2007. during september and october 2011 the north coast public health unit conducted a household study of secondary attack rates to assess the effectiveness of per ... | 2015 | 26063095 |
| bordetella pertussis isolates circulating in china where whole cell vaccines have been used for 50 years. | | 2015 | 26060284 |
| [vaccinations from the pulmonologist's point of view]. | the best strategy for prevention of acute respiratory tract infections is primary prophylaxis against diseases preventable by vaccination. from the pulmonologist's point of view, vaccinations against pneumococci, influenza a and b viruses and bordetella pertussis are of particular clinical relevance. | 2015 | 26059891 |
| whooping cough, twenty years from acellular vaccines introduction. | clinical pertussis resulting from infection with b. pertussis is a significant medical and public health problem, despite the huge success of vaccination that has greatly reduced its incidence. the whole cell vaccine had an undeniable success over the last 50 years, but its acceptance was strongly inhibited by fear, only partially justified, of severe side effects, but also, in the western world, by the difficulty to enter in combination with other vaccines: today multi-vaccine formulations are ... | 2015 | 26051141 |
| competition, coinfection and strain replacement in models of bordetella pertussis. | pertussis, or whooping cough, is an important respiratory infection causing considerable infant mortality worldwide. recently, incidence has risen in countries with strong vaccine programmes and there are concerns about antigenic shift resulting in vaccine evasion. interactions between pertussis and non-vaccine-preventable strains will play an important role in the evolution and population dynamics of pertussis. in particular, if we are to understand the role strain replacement plays in vaccinat ... | 2015 | 26032910 |
| a versatile, non genetically modified organism (gmo)-based strategy for controlling low-producer mutants in bordetella pertussis cultures using antigenic modulation. | the uncontrolled presence of non-producer mutants negatively affects bioprocesses. in bordetella pertussis cultures, avirulent mutants emerge spontaneously and accumulate. we characterized the dynamics of accumulation using high-throughput growth assays and competition experiments between virulent and avirulent (bvg(-) ) isolates. a fitness advantage of bvg(-) cells was identified as the main driver for bvg(-) accumulation under conditions of high virulence factor production. conversely, under c ... | 2015 | 26014907 |
| immunoproteomic profiling of bordetella pertussis outer membrane vesicle vaccine reveals broad and balanced humoral immunogenicity. | the current resurgence of whooping cough is alarming, and improved pertussis vaccines are thought to offer a solution. outer membrane vesicle vaccines (omvpv) are potential vaccine candidates, but omvpv-induced humoral responses have not yet been characterized in detail. the purpose of this study was to determine the antigen composition of omvpv and to elucidate the immunogenicity of the individual antigens. quantitative proteome analysis revealed the complex composition of omvpv. the omvpv immu ... | 2015 | 25988566 |
| pertussis resurgence associated with pertactin-deficient and genetically divergent bordetella pertussis isolates in israel. | the bordetella pertussis polymerase chain reaction positivity rate changed after additional diphtheria-tetanus-acellular pertussis boosters in 2005 and 2008, 9.8%, 13.4%, 22% and 15.2% in 2010, 2011, 2012 and 2013, p < 0.001, respectively. new pulsed-field gel electrophoresis profiles were detected between 2009 and 2012. the proportion of pertactin-deficient isolates increased over time, 6.6% versus 7.1% versus 33.3% during 2005-2006, 2011-2012 and 2013-2014, p < 0.03, respectively. | 2015 | 25966915 |
| host-pathogen interaction during bacterial vaccination. | vaccines have been developed and deployed against several important bacterial pathogens of humans, including neisseria meningitidis, bordetella pertussis, streptococcus pneumoniae and mycobacterium tuberculosis. these vaccines are generally considered a successful public health measure and are effective at controlling disease symptoms and/or burden. however, a troubling consequence of recent vaccination programs has been the selection of vaccine escape mutants, whereby the pathogen displays a di ... | 2015 | 25966310 |
| structural and functional studies of bapc protein of bordetella pertussis. | bordetella pertussis, the causative agent of whooping cough, attaches to mucosal surface in upper respiratory tract, where it produces a variety of surface associated and secreted autotransporter molecules among others. in this study we have cloned newly identified member of autotransporter family bapc (b. pertussis autotransporter protein c); expressed it in escherichia coli and characterized it for its different properties. we have also raised antisera to bapc protein; the antisera were used i ... | 2015 | 25946329 |
| bordetella pertussis infection in paediatric healthcare workers. | an increased incidence of pertussis has been observed recently in adults, and healthcare workers (hcws) are considered a risk group for transmission to infants. prevalence of recent pertussis infection was assessed in hcws from a paediatric department of a tertiary care hospital in brazil. serum pertussis toxin igg antibodies were measured by enzyme-linked immunosorbent assay. of 388 hcws included in the analysis, 6.4% had serology suggestive of recent infection. medical residents [odds ratio (o ... | 2015 | 25913647 |
| bordetella pertussis adenylate cyclase toxin blocks induction of bactericidal nitric oxide in macrophages through camp-dependent activation of the shp-1 phosphatase. | the adenylate cyclase toxin-hemolysin (cyaa) plays a key role in the virulence of bordetella pertussis. cyaa penetrates complement receptor 3-expressing phagocytes and catalyzes uncontrolled conversion of cytosolic atp to the key second messenger molecule camp. this paralyzes the capacity of neutrophils and macrophages to kill bacteria by complement-dependent oxidative burst and opsonophagocytic mechanisms. we show that camp signaling through the protein kinase a (pka) pathway activates src homo ... | 2015 | 25876760 |
| minimum inhibitory concentrations of erythromycin and other antibiotics for czech strains of bordetella pertussis. | to test the susceptibility to first-line and alternative antibiotics of 70 bordetella pertussis (b. pertussis) strains recovered from patients with whooping cough through national pertussis surveillance in the czech republic (cr) in 1967-2010. | 2015 | 25872990 |
| bordetella pertussis infection among international travellers: the need for a rigorous implementation of vaccine policies. | | 2015 | 25864792 |
| molecular epidemiology of bordetella pertussis in the philippines in 2012-2014. | the present study was designed to determine the genotypes of circulating bordetella pertussis in the philippines by direct molecular typing of clinical specimens. | 2015 | 25861926 |
| importance of enhanced surveillance for prevention of pertussis in children. | bordetella pertussis infection causes considerable morbidity, even in countries with high vaccination coverage. surveillance of pertussis is usually passive and based on mandatory reporting. we assessed the epidemiologic and clinical characteristics of pertussis cases detected by passive or enhanced surveillance. | 2015 | 25860533 |
| biochemical and functional characterization of a periplasmic disulfide oxidoreductase from neisseria meningitidis essential for meningococcal viability. | tlpas (thioredoxin-like proteins) are bacterial thioredoxin-like periplasmic disulfide oxidoreductases generally involved in cytochrome c maturation (ccm) process. they contain a characteristic cxxc active site motif involved in disulfide exchange reaction. in the human pathogenic neisseria meningitidis species, no tlpa has been characterized so far. in the present study, using an in silico analysis, we identified a putative periplasmic tlpa, called tlpa2. biochemical and kinetic characterizatio ... | 2015 | 25826614 |
| implementation of pertussis immunization in health-care personnel. | infection with bordetella pertussis is most severe in young infants who frequently acquire it from adults. pertussis immunization in adults 25-29 years of age and all adults in close contact with infants <6 months was introduced in switzerland in 2012. we immediately implemented this new recommendation in our hospital with a vaccination campaign. | 2015 | 25776922 |
| [pertussis in mexico, an epidemiological overview. a study of 19 years at the instituto mexicano del seguro social]. | bordetella pertussis infection remains a public health problem in several developed and developing we describe the epidemiological syndrome cases subsystem special surveillance of whooping cough from 1992 to 2011 at a population with social security. | 2016 | 25760745 |
| shotgun proteome analysis of bordetella pertussis reveals a distinct influence of iron availability on the bacterial metabolism, virulence, and defense response. | one of the mechanisms involved in host immunity is the limitation of iron accessibility to pathogens, which in turn provokes the corresponding physiological adaptation of pathogens. this study reports a gel-free nanolc-ms/ms-based comparative proteome analysis of bordetella pertussis grown under iron-excess and iron-depleted conditions. out of the 926 proteins covered 98 displayed a shift in their abundance in response to low iron availability. forty-seven of them were found to be increased in l ... | 2015 | 25755163 |
| uptake of a government-funded pertussis-containing booster vaccination program for parents of new babies in victoria, australia. | an epidemic of bordetella pertussis in victoria, australia, led to the implementation of a government-funded vaccination program for parents of new babies. the rationale was to protect unimmunised infants from infection by vaccinating parents with a pertussis-containing vaccine. this is known as cocooning. | 2015 | 25728321 |
| where macrolide resistance is prevalent. | | 2015 | 25703275 |
| investigating pertussis toxin and its impact on vaccination. | whooping cough, caused by bordetella pertussis, remains a major global health problem. each year around 40 million of pertussis cases resulting in 200,000-400,000 annual deaths occur worldwide. pertussis toxin is a major virulence factor of b. pertussis. murine studies have shown its importance in bacterial colonization and in immunomodulation to evade innate or adaptive immunity. the toxin is composed of an a protomer expressing adp-ribosyltransferase activity and a b oligomer, responsible for ... | 2015 | 25689536 |
| structure and proposed mechanism of l-α-glycerophosphate oxidase from mycoplasma pneumoniae. | the formation of h2 o2 by the fad-dependent l-α-glycerophosphate oxidase (glpo) is important for the pathogenesis of streptococcus pneumoniae and mycoplasma pneumoniae. the structurally known glpo from streptococcus sp. (sspglpo) is similar to the pneumococcal protein (spglpo) and provides a guide for drug design against that target. however, m. pneumoniae glpo (mpglpo), having < 20% sequence identity with structurally known glpos, appears to represent a second type of glpo that we designate as ... | 2015 | 25688572 |
| [recent epidemiology of pertussis: implications for vaccination]. | the development of acellular pertussis vaccines allowed since more than a decade to continue beyond the 2 first years of life the immunization against this illness. this technological progress should have led to an improvement in the control of the disease. however, despite high immunisation rates in infancy, bordetella pertussis is still circulating in the population. since 2010 a marked increase in the number of cases of whooping cough in infants too young to be vaccinated, as well as in older ... | 2014 | 25675639 |
| modulation of the cd4(+) t cell response after acellular pertussis vaccination in the presence of tlr4 ligation. | whole cell pertussis (wp) vaccines are gradually being replaced by aluminum salt-adjuvanted acellular pertussis (ap) vaccines. these promote cd4(+) t cell responses with a non-protective th2 component, while protective immune mechanisms to b. pertussis may rather involve long-lived th1/th17 type cd4(+) t cells. here we asked whether addition of a non-toxic meningococcal lps derivative, lpxl1, as adjuvant can favorably modulate the ap-induced pertussis-specific cd4(+) t cell response in mice. to ... | 2015 | 25659267 |
| in vitro innate immune cell based models to assess whole cell bordetella pertussis vaccine quality: a proof of principle. | lot release testing of vaccines is primarily based on animal models that are costly, time-consuming and sometimes of questionable relevance. in order to reduce animal use, functional in vitro assays are being explored as an alternative approach for the current lot release testing paradigm. in this study, we present an evaluation of apc platforms assessing innate immune activation by whole cell bordetella pertussis (wp) vaccines. primary monocytes, monocyte-derived dc (modc) and human monocyte/dc ... | 2015 | 25633359 |
| antibody levels to bordetella pertussis and neisseria meningitidis in immunodeficient patients receiving immunoglobulin replacement therapy. | patients with antibody deficiency rely on immunoglobulin products for protection against many vaccine-preventable diseases. we measured antibody titers against bordetella pertussis and neisseria meningitidis in patients receiving immunoglobulin (ig) therapy to determine if they have any protection against infections from these organisms. | 2015 | 25631528 |
| [the status of pertussis infection and molecular epidemiological characteristics of pertussis in tianjin, 2013]. | to understand the status of pertussis infection and characteristics of molecular epidemiology of pertussis in 2013 in tianjin. | 2014 | 25623454 |
| resurgence of pertussis at the age of vaccination: clinical, epidemiological, and molecular aspects. | report the incidence, epidemiology, clinical features, death, and vaccination status of patients with whooping cough and perform genotypic characterization of isolates of b. pertussis identified in the state of paraná, during january 2007 to december 2013. | 2015 | 25623040 |
| pertussis in the newborn: certainties and uncertainties in 2014. | bordetella pertussis infection remains a serious potential health risk to infants, specially in those too young to be vaccinated. over the recent years, numerous sources highlighted a widespread resurgence, making it, again, a challenging disease. globally, pertussis is ranked among the 10 leading causes of childhood mortality. this review summarizes the most recent literature and will address the most important aspects that pediatricians and neonatologists must be familiar with, when treating a ... | 2015 | 25613084 |
| notes from the field: investigation of contacts of a health care worker who worked while ill with pertussis--maryland, august-september 2014. | on september 5, 2014, the public health department of a maryland hospital was notified of a case of bordetella pertussis infection confirmed by polymerase chain reaction (pcr) in a staff health care worker (hcw). the hcw experienced onset of diarrhea and malaise (nonrespiratory symptoms atypical of the catarrhal phase of pertussis) on august 26. by september 2, paroxysms of coughing led the hcw to consult a colleague, who ordered the pcr test, prescribed a 5-day course of azithromycin, and advis ... | 2015 | 25611170 |
| comparison of loop-mediated isothermal amplification and real-time pcr for detecting bordetella pertussis. | | 2015 | 25596118 |
| carrot cells: a pioneering platform for biopharmaceuticals production. | carrot (daucus carota l.) is of importance in the molecular farming field as it constitutes the first plant species approved to produce biopharmaceuticals for human use. in this review, features that make carrot an advantageous species in the molecular farming field are analyzed and a description of the developments achieved with this crop thus far is presented. a guide for genetic transformation procedures is also included. the state of the art comprises ten vaccine prototypes against measles v ... | 2015 | 25572939 |
| interaction of bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding. | the interaction of bordetella pertussis adenylate cyclase toxin (cyaa) with complement receptor 3 (cr3, cd11b/cd18) involves n-linked oligosaccharide chains. to investigate the relative importance of the individual n-glycans of cr3 for toxin activity, the asparagine residues of the consensus n-glycosylation sites of cr3 were substituted with glutamine residues that cannot be glycosylated. examination of cr3 mutant variants and mass spectrometry analysis of the n-glycosylation pattern of cr3 reve ... | 2015 | 25554420 |
| epidemiology of pertussis in adolescents and adults in turkey. | two hundred and fourteen patients who had a cough illness lasting at least 2 weeks were studied to investigate bordetella pertussis as a cause of prolonged cough in adolescents and adults. medical history and nasopharyngeal swab specimens for culture and polymerase chain reaction (pcr) were obtained at presentation. three (1·4%) patients were b. pertussis culture-positive; 15 (7%) were b. pertussis pcr-positive (including the culture-positive patients) and 11 (5·1%) were bordetella spp. pcr-posi ... | 2015 | 25524454 |
| filamentous hemagglutinin of bordetella pertussis: a key adhesin with immunomodulatory properties? | the filamentous hemagglutinin of pathogenic bordetellae is a prototype of a large two-partner-system-secreted and β-structure-rich bacterial adhesin. it exhibits several binding activities that may facilitate bacterial adherence to airway mucosa and host phagocytes in the initial phases of infection. despite three decades of research on filamentous hemagglutinin, there remain many questions on its structure-function relationships, integrin interactions and possible immunomodulatory signaling cap ... | 2014 | 25517899 |
| a critical role for the tlr signaling adapter mal in alveolar macrophage-mediated protection against bordetella pertussis. | bordetella pertussis causes whooping cough, an infectious disease of the respiratory tract that is re-emerging despite high vaccine coverage. here we examined the role of toll-like receptor (tlr) adapter protein mal in the control of b. pertussis infection in the lungs. we found that b. pertussis bacterial load in the lungs of mal-defective (mal(-/-)) mice exceeded that of wild-type (wt) mice by up to 100-fold and bacteria disseminated to the liver in mal(-/-) mice and 50% of these mice died fro ... | 2015 | 25515629 |
| genomic analysis of isolates from the united kingdom 2012 pertussis outbreak reveals that vaccine antigen genes are unusually fast evolving. | a major outbreak of whooping cough, or pertussis, occurred in 2012 in the united kingdom (uk), with nearly 10 000 laboratory-confirmed cases and 14 infant deaths attributed to pertussis. a worldwide resurgence of pertussis has been linked to switch to the use of acellular pertussis vaccines and the evolution of bordetella pertussis away from vaccine-mediated immunity. we have conducted genomic analyses of multiple strains from the uk outbreak. we show that the uk outbreak was polyclonal in natur ... | 2015 | 25489002 |
| dominance of two genotypes of bordetella pertussis during a period of increased pertussis activity in alberta, canada: january to august 2012. | the purpose of this study was to undertake an epidemiological analysis of an increase in bordetella pertussis activity during the period january 1 to august 31, 2012 in alberta, canada. b. pertussis testing was done using an is481 real-time pcr assay with pcr-positive and indeterminate specimens cultured and stored for further analysis. | 2014 | 25462185 |
| pertussis may be the cause of prolonged cough in adolescents and adults in the interepidemic period. | this study was aimed to evaluate the prevalence of pertussis in adolescents and adults with cough lasting more than 14 days and less than 30 days. | 2015 | 25452019 |
| pertussis serosurveillance study in izmir, turkey. | pertussis is a life-threatening, vaccine-preventable infection. adults who can be asymptomatic may infect infants. the aim of this study is to determine the igg antibody levels against pertussis toxin (pt) and filamentous hemagglutinin from 6 months to ≥60 years in izmir, turkey. a cluster sample design developed by expanded programme on immunization of the world health organization was carried out for the selection of the study population, which consisted of 399 healthy subjects. in-house elisa ... | 2015 | 25424662 |
| sources of pertussis infection in young babies from são paulo state, brazil. | household contacts are important sources of bordetella pertussis in infants. a total of 353 household contacts of 97 index cases were evaluated for pertussis by culture and polymerase chain reaction. twenty eight contacts were positive (8.0%). the presence of symptoms did not influence the rate of diagnosed bacteriologic pertussis in communicants. we conclude that contacts with an index case can be positive for b. pertussis independently of the presence of symptoms. | 2014 | 25386966 |
| seroprevalence of pertussis antibodies in 6-17-year-old students in ahvaz, south-west islamic republic of iran. | although pertussis is a vaccine-preventable infection, vaccine-induced immunity is not lifelong and booster doses are recommended according to national disease epidemiology. the aim of this study was to evaluate pertussis-igg levels in school-aged students in ahvaz, south-west islamic republic of iran. in a descriptive, cross-sectional study, blood samples were obtained from 640 students (382 boys and 258 girls) aged 6-17 years during 2010-2011. all students had received a full course of pertuss ... | 2014 | 25356693 |
| interaction of bordetella pertussis filamentous hemagglutinin with human tlr2: identification of the tlr2-binding domain. | filamentous hemagglutinin (fha) is a major adhesion and virulence factor of bordetella pertussis and also a main component of acellular pertussis vaccines. interaction of fha with different receptors on human epithelial and immune cells facilitates entrance and colonization of bacteria as well as immunomodulation of the host immune response. three overlapping segments of the fha gene were cloned in a prokaryotic expression vector and the recombinant proteins were purified. these recombinant frag ... | 2015 | 25353353 |
| [clinical characteristics of whooping cough in neonates and antimicrobial resistance of the pathogenic bacteria]. | to study the clinical characteristics of whooping cough in neonates and the antimicrobial resistance of the bacterial isolates. | 2014 | 25344174 |
| translocation path of a substrate protein through its omp85 transporter. | tpsb proteins are omp85 superfamily members that mediate protein translocation across the outer membrane of gram-negative bacteria. omp85 transporters are composed of n-terminal potra domains and a c-terminal transmembrane β-barrel. in this work, we track the in vivo secretion path of the bordetella pertussis filamentous haemagglutinin (fha), the substrate of the model tpsb transporter fhac, using site-specific crosslinking. the conserved secretion domain of fha interacts with the potra domains, ... | 2014 | 25327833 |
| [epidemiologic and clinical characteristics of children with disease due to bordetella pertussis in santa fe, argentina]. | pertussis, a vaccine-preventable respiratory disease, remains a public health problem. | 2014 | 25327190 |
| a novel tlr2 agonist from bordetella pertussis is a potent adjuvant that promotes protective immunity with an acellular pertussis vaccine. | bordetella pertussis causes whooping cough, a severe and often lethal respiratory infection in infants. a recent resurgence of pertussis has been linked with waning or suboptimal immunity induced with acellular pertussis vaccines (pa) that were introduced to most developed countries in the 1990s because of safety concerns around the use of whole-cell pertussis vaccines (pw). pa are composed of individual b. pertussis antigens absorbed to alum and promote strong antibody, t helper type 2 (th2) an ... | 2015 | 25315966 |
| whooping cough. | whooping cough or pertussis is a contagious disease of the upper respiratory tract caused by the bacterium bordetella pertussis. whooping cough is transmitted via droplets in the air from sneezing or coughing and individuals are considered infectious from just before and up to 21 days after the onset of the cough. usually it has an incubation period of seven to ten days, and the disease lasts six to eight weeks. | 2014 | 25315570 |
| pertactin deficient bordetella pertussis present a better fitness in mice immunized with an acellular pertussis vaccine. | bordetella pertussis is the etiologic agent of whooping cough and has been the target of vaccination for over fifty years. the latest strategies include the use of acellular pertussis vaccines that induce specific immunity against few virulence factors amongst which pertactin is included in three and five component acellular pertussis vaccines. recently, it has been reported that b. pertussis clinical isolates loose the production of this adhesin in regions reaching high vaccine coverage with va ... | 2014 | 25312274 |
| pertactin-negative bordetella pertussis strains: evidence for a possible selective advantage. | a recent increase in bordetella pertussis without the pertactin protein, an acellular vaccine immunogen, has been reported in the united states. determining whether pertactin-deficient (prn(-)) b. pertussis is evading vaccine-induced immunity or altering the severity of illness is needed. | 2015 | 25301209 |
| colonization of bordetella pertussis clinical isolates that differ by pulsed field gel electrophoresis types in the lungs of naïve mice or mice immunized with the whole-cell pertussis vaccine used in poland. | the goal of our study was to compare the elimination of bordetella pertussis clinical isolates that differ according to pulsed field gel electrophoresis (pfge), serotypes and genes encoding virulence factors from the lungs of naïve mice or mice immunized with commercial diphtheria-tetanus-whole-cell pertussis vaccine used in poland. when a mixture of four isolates, given in equal proportions and harboring different pfge profiles, serotypes, and alleles encoding virulence factors, was used to inf ... | 2015 | 25297678 |
| [vaccinations in pneumology]. | the best measure to prevent infections of the respiratory tract is the use of vaccines against important respiratory tract pathogens. the standing vaccination committee at the robert koch institute recommends not only children but also adult vaccination against pneumococci, influenza a/b and bordetella pertussis, because of a high disease burden. in the present review the clinical significance and safety of those vaccinations as well as advantages and disadvantages of the currently available vac ... | 2014 | 25290919 |
| [immunogenicity and safety of vaccine preparations based on circulating bordetella pertussis strains]. | study specific activity and safety ofvaccine preparations based on circulating b. pertussis strains with currently predominating allele variants of pertussis toxin (ptxa1) and pertactin (prn2) genes. | 2014 | 25286523 |
| generation and use of site-directed chromosomal cyaa' translational fusions in salmonella enterica. | cyaa from bordetella pertussis is a calmodulin-dependent adenylate cyclase. fusions to the catalytic domain of cyaa (cyaa') are useful tools to detect translocation of type iii secretion system effectors from gram-negative pathogens like salmonella enterica. these fusions are usually generated using plasmids with strong promoters. here, we describe a protocol to insert the cyaa'-encoding sequence in a specific site in the bacterial chromosome in order to get a monocopy fusion whose expression is ... | 2015 | 25253250 |
| identification of linear b epitopes of pertactin of bordetella pertussis induced by immunization with whole and acellular vaccine. | pertussis is a serious infectious disease of the respiratory tract caused by the gram-negative bacteria bordetella pertussis. there has been a reemergence of this disease within the population of several countries that have well established vaccination programs. analyzes of clinical isolates suggest an antigenic divergence between the vaccine-based strains to the circulating strains. although antibodies against p.69 are involved in the observed protective immunity, the sequences recognized as an ... | 2014 | 25252193 |
| mutation in the β-hairpin of the bordetella pertussis adenylate cyclase toxin modulates n-lobe conformation in calmodulin. | bordetella pertussis, causative agent of whooping cough, produces an adenylate cyclase toxin (cyaa) that is an important virulence factor. in the host cell, the adenylate cyclase domain of cyaa (cyaa-acd) is activated upon association with calmodulin (cam), an ef-hand protein comprised of n- and c-lobes (n-cam and c-cam, respectively) connected by a flexible tether. maximal cyaa-acd activation is achieved through its binding to both lobes of intact cam, but the structural mechanisms remain uncle ... | 2014 | 25251320 |
| antibiotic treatment of pertussis: are 7 days really sufficient? | short courses of antibiotic therapy are recommended for treatment of pertussis. we report 2 young unvaccinated infants with persistently positive bordetella pertussis by polymerase chain reaction from nasopharyngeal swabs despite 7 days of clarithromycin (15 mg/kg/d) therapy. in 1 patient, quantitative polymerase chain reaction was 7.02 (log geq/ml) at the onset of treatment, 6.26 at the end of treatment and remained positive with 2.64 and 2.69 during and after a second 7-day course, respectivel ... | 2015 | 25247586 |
| pertactin-negative bordetella pertussis strains in canada: characterization of a dozen isolates based on a survey of 224 samples collected in different parts of the country over the last 20 years. | to detect and characterize pertactin-negative bordetella pertussis in canada, especially for isolates collected in recent years. | 2014 | 25244999 |
| clinical manifestations of children with microbiologically confirmed pertussis infection and antimicrobial susceptibility of isolated strains in a regional hospital in japan, 2008-2012. | we conducted a retrospective study in 57 children (median age, 3.5 years; range, 1 month-14.5 years) with microbiologically confirmed pertussis infection over a recent 4-year period in a regional hospital in japan. we obtained nasal swabs from all patients for bordetella pertussis isolation as well as performed b. pertussis dna detection using loop-mediated isothermal amplification (lamp). of the 57 cases, 34 (60%) were culture-positive and 57 (100%) were lamp-positive. the frequency of each sym ... | 2014 | 25241683 |
| characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles. | pertussis has resurged during the last two decades in different countries. in particular in the 2010-2013 period large outbreaks were detected in us, australia, uk and the netherlands with significant mortality in infants. the epidemiological situation of pertussis points out the need to develop new vaccines and in this regard we previously developed a new vaccine based on outer membrane vesicles (omvs) which have been shown to be safe and to induce protection in mice. here we have further inves ... | 2014 | 25240753 |
| acellular vaccines for preventing whooping cough in children. | routine use of whole-cell pertussis (wp) vaccines was suspended in some countries in the 1970s and 1980s because of concerns about adverse effects. following this action, there was a resurgence of whooping cough. acellular pertussis (ap) vaccines, containing purified or recombinant bordetella pertussis (b. pertussis) antigens, were developed in the hope that they would be as effective, but less reactogenic than the whole-cell vaccines. this is an update of a cochrane review first published in 19 ... | 2014 | 25228233 |
| pertussis in young infants: clinical presentation, course and prevention. | pertussis is a highly contagious disease caused by the gram negative aerobic coccobacillus, bordetella pertussis. it may present with severe symptoms and complications in infants and can pose a diagnostic challenge. this is a vaccine preventable illness covered by the irish childhood immunisation schedule. in 2011, a retrospective review was conducted of the records of infants, under six months, with a confirmed diagnosis of pertussis, presenting to temple street children's university hospital ( ... | 2014 | 25226721 |
| comment on: seroprevalence of bordetella pertussis antibody in pregnant women in iran. | | 2014 | 25209914 |
| genetically detoxified pertussis toxin (pt-9k/129g): implications for immunization and vaccines. | pertussis toxin (pt) is one of the major virulence factors of bordetella pertussis and the primary component of all pertussis vaccines available to date. because of its various noxious effects the toxin needs to be detoxified. in all currently available vaccines, detoxification is achieved by treatment with high quantity of chemical agents such as formaldehyde, glutaraldehyde or hydrogen peroxide. although effective in detoxification, this chemical treatment alters dramatically the immunological ... | 2014 | 25183193 |
| [etiology of community-acquired pneumonia in children hospitalized in the period of 2009-2012]. | the aim of this study was to analyze etiology of childhood pneumonia in the region of warsaw and to assess the efficacy and usefulness of standard diagnostic procedures used in search of etiology. | 2014 | 25182260 |
| investigations into the emergence of pertactin-deficient bordetella pertussis isolates in six european countries, 1996 to 2012. | pathogen adaptation has been proposed to contribute to the resurgence of pertussis. a striking recent example is the emergence of isolates deficient in the vaccine component pertactin (prn). this study explores the emergence of such prn-deficient isolates in six european countries. during 2007 to 2009, 0/83 isolates from the netherlands, 0/18 from the united kingdom, 0/17 finland, 0/23 denmark, 4/99 sweden and 5/20 from norway of the isolates collected were prn-deficient. in the netherlands and ... | 2014 | 25166348 |
| pertussis in latin america: epidemiology and control strategies. | pertussis is a serious respiratory disease in infants that can also affect children and adults. vaccination against pertussis was introduced in the 1950s and in the 1990s a resurgence of pertussis was observed worldwide. the aim of this work is to summarize the recent data concerning pertussis disease in different countries of latin america. in this geographic region, pertussis is nationally notifiable and cases should be reported to the appropriate health department/ministry. though the surveil ... | 2014 | 25139010 |
| differential ova-induced pulmonary inflammation and unspecific reaction in dark agouti (da) rats contingent on cd26/dppiv deficiency. | many disease models have shown that, within the species rat, different strains are differentially susceptible to asthma-induced inflammation depending on the genetic background. likewise, cd26/dppiv-deficiency in asthmatic f344 rats has been shown to result in a less pronounced inflammation and in increased treg cell influx into the lung compared to wild-types. the aim of the present study was to investigate whether the genetic background of the animals interferes with cd26/dppiv-deficiency in a ... | 2014 | 25108564 |
| cnmp-ams mimic and dissect bacterial nucleotidyl cyclase toxin effects. | in addition to the well-known second messengers camp and cgmp, mammalian cells contain the cyclic pyrimidine nucleotides ccmp and cump. the pseudomonas aeruginosa toxin exoy massively increases cgmp and cump in cells, whereas the bordetella pertussis toxin cyaa increases camp and, to a lesser extent, ccmp. to mimic and dissect toxin effects, we synthesized cnmp-acetoxymethylesters as prodrugs. cnmp-ams rapidly and effectively released the corresponding cnmp in cells. the combination of cgmp-am p ... | 2014 | 25108158 |
| bordetella pertussis fimbriae (fim): relevance for vaccines. | bordetella pertussis produces two serologically distinct fimbriae, fim2 and fim3. expression of these antigens is governed by the bvga/s system and by the length of a poly(c) tract in the promoter of each gene. fim2 and fim3 are important antigens for whole cell pertussis vaccines as clinical trials have shown an association of anti-fimbriae antibody-mediated agglutination and protection. the current five component acellular pertussis vaccine contains co-purified fim2/3 and provided good efficac ... | 2014 | 25102891 |
| seroprevalence of igg antibodies to pertussis toxin in children and adolescents in estonia. | despite high immunisation coverage and frequent booster doses, the national notification rates of pertussis in estonia have been increasing. the peak of 97/100,000 was reached in 2010 which is the highest incidence rate since 1962 (210/100,000). we aimed to measure the prevalence of pertussis toxin (pt) igg type antibodies in subjects of <18 years and to estimate the pertussis infection activity in a recently non-immunised cohort. | 2014 | 25093282 |
| sequence variation in virulence-related genes of bordetella pertussis isolates from poland in the period 1959-2013. | this study aimed to characterise bordetella pertussis isolates circulating in poland since 1959. sequence analysis of ptxa, ptxc, prn, tcfa, fim2, fim3 and ptxp for 175 clinical isolates and currently and previously used vaccine strains was performed. clinical isolates from the period 1995-2013 were found to be different to three currently used vaccine strains harbouring the allelic combination ptxa2-ptxc1-ptxp1-prn1-tcfa2-fim2-1-fim3-1, seen frequently in poland in the early pertussis vaccinati ... | 2015 | 25090968 |
| adenylate cyclase toxin-hemolysin relevance for pertussis vaccines. | the adenylate cyclase toxin-hemolysin (act, ac-hly or cyaa) is a key virulence factor of bordetella pertussis. it targets bactericidal activities of phagocytes, such as oxidative burst and complement- or antibody-mediated opsonophagocytic killing of bacteria. through camp signaling, cyaa also skews tlr-triggered maturation of dendritic cells, inhibiting proinflammatory il-12 and tnf-α secretion and enhancing il-10 production and treg expansion, likely hampering induction of adaptive immune respo ... | 2014 | 25090574 |
| shortcomings of pertussis vaccines: why we need a third generation vaccine. | first generation whole-cell (wp) and second generation acellular (ap) pertussis vaccines have been highly effective in preventing childhood deaths due to pertussis, yet both vaccines have drawbacks that have limited their long-term usefulness. these include issues of reactogenicity and potency in the case of wp, and limited durability of protection and the potential for selection of escape mutants in the case of ap. neither vaccine prevents disease in neonatal infants who continue to die from pe ... | 2014 | 25089373 |
| importance of (antibody-dependent) complement-mediated serum killing in protection against bordetella pertussis. | pertussis is a highly contagious respiratory disease that is caused by bordetella pertussis. despite being vaccine preventable, pertussis rates have been rising steadily over the last decades, even in areas with high vaccine uptake. recently, experiments with infant baboons indicated that although vaccination with acellular pertussis vaccines prevented disease, no apparent effect was observed on infection and transmission. one explanation may be that current acellular pertussis vaccines do not i ... | 2014 | 25081731 |
| notes from the field: outbreak of pertussis in a school and religious community averse to health care and vaccinations--columbia county, florida, 2013. | on august 30, 2013, the florida department of health in columbia county was notified of a bordetella pertussis laboratory-positive unimmunized child attending a local charter school (316 students from pre-k through 8th grade) in a large religious community averse to health care and vaccinations. kindergarten immunization records showed that only five (15%) of 34 students were fully immunized with pertussis-antigen-containing vaccines. in seventh grade, only one (5%) of 22 students was fully immu ... | 2014 | 25078655 |
| neonatal pertussis, cocooning and maternal immunization. | the rising incidence of whooping cough, a highly contagious infection caused by bordetella pertussis, is particularly significant for young infants who have the highest risk for morbidity and mortality. the pertussis resurgence has led to a shift in primary prevention relying on childhood vaccination to a cocooning strategy, that is, vaccination of close contacts of newborn infants (new mothers, fathers, grandparents, siblings, caretakers, etc.), thereby reducing pertussis exposure. immunization ... | 2014 | 25075629 |
| [mechanisms of formation of post-vaccinal immune response in children immunized with apdt and adt-m preparations]. | study the mechanisms of formation of cell and humoral immunity against pertussis, diphtheria and tetanus in children immunized with immunobiological preparations (apdt vaccine and adt anatoxin). | 2014 | 25051700 |
| other bordetellas, lessons for and from pertussis vaccines. | the bordetella genus comprises nine species of which bordetella pertussis and b. parapertussis are isolated from humans and are the most studied bordetella species since they cause whooping cough. they both originate from b. bronchiseptica, which infects several mammals and immune compromised humans, but the intensive use of pertussis vaccines induced changes in b. pertussis and b. parapertussis populations. b. petrii and b. holmesii are other species of unknown reservoir and transmission patter ... | 2014 | 25034039 |
| the mouse intranasal challenge model for potency testing of whole-cell pertussis vaccines. | a mouse intracerebral challenge model is used for potency testing of whole-cell pertussis (wp) vaccines. we investigated the use of a mouse nasopharyngeal challenge model, which better reflects the clinical features of pertussis disease, to differentiate between efficacy of wp vaccines. | 2014 | 25029905 |
| plasticity of fimbrial genotype and serotype within populations of bordetella pertussis: analysis by paired flow cytometry and genome sequencing. | the fimbriae of bordetella pertussis are required for colonization of the human respiratory tract. two serologically distinct fimbrial subunits, fim2 and fim3, considered important vaccine components for many years, are included in the sanofi pasteur 5-component acellular pertussis vaccine, and the world health organization recommends the inclusion of strains expressing both fimbrial serotypes in whole-cell pertussis vaccines. each of the fimbrial major subunit genes, fim2, fim3, and fimx, has a ... | 2014 | 25028460 |
| antigen targeting to cd11b+ dendritic cells in association with tlr4/trif signaling promotes strong cd8+ t cell responses. | deciphering the mechanisms that allow the induction of strong immune responses is crucial to developing efficient vaccines against infectious diseases and cancer. based on the discovery that the adenylate cyclase from bordetella pertussis binds to the cd11b/cd18 integrin, we developed a highly efficient detoxified adenylate cyclase-based vector (cyaa) capable of delivering a large variety of ags to the apc. this vector allows the induction of protective and therapeutic immunity against viral and ... | 2014 | 25024388 |
| improved pertussis vaccines based on adjuvants that induce cell-mediated immunity. | bordetella pertussis is a gram-negative bacterium that causes the severe and sometimes lethal respiratory disease whooping cough in infants and children. there has been a recent resurgence in the number of cases of pertussis in several countries with high vaccine coverage. this has been linked with waning or ineffective immunity induced by current acellular pertussis vaccines. these acellular pertussis vaccines are formulated with alum as the adjuvant, which promotes strong antibody responses bu ... | 2014 | 25017925 |
| pathogenesis and histopathology of pertussis: implications for immunization. | pertussis is a unique infectious disease in that it can be severe and fatal but occurs without fever and other evidence of an inflammatory illness. the authors with others have studied the histopathology of fatal pertussis and also the unique characteristics of severe pertussis in young infants. histopathologic observations from approximately 100 years ago, and from recent evaluation, indicate that the histopathologic changes of the upper respiratory tract of patients with fatal pertussis are of ... | 2014 | 24992986 |
| adult pertussis in the pre- and post-vaccine eras: lifelong vaccine-induced immunity? | in the pre-vaccine era, there was little information about clinical pertussis in adults, although deaths were noted. today bordetella pertussis infection in adults is noted to cause a full spectrum of clinical manifestations. if single serum serologic diagnosis becomes routine, the finding of adult cases would increase dramatically. reported adult pertussis was rare in the pre-vaccine era and it has been increasing markedly in the present era. in the present vaccine era, data on adult pertussis ... | 2014 | 24985069 |
| a gateway(®) -compatible bacterial adenylate cyclase-based two-hybrid system. | the bacterial adenylate cyclase two-hybrid (bacth) system has been widely used to characterize protein-protein interactions in the prokaryotic world. this system relies on the interaction-mediated reconstitution of adenylate cyclase activity in escherichia coli by bringing together two complementary fragments of the catalytic domain of the adenylate cyclase toxin of bordetella pertussis. a limiting factor in performing large-scale two-hybrid interaction screens with full-length open reading fram ... | 2014 | 24983530 |
| [anesthetic management of a patient with severe subcutaneous and mediastinal emphysema due to pertussis infection]. | a 20-month-old girl, with respiratory failure due to severe subcutaneous and mediastinal emphysema, was scheduled to undergo percutaneous drainage of emphysema and induction of extracorporeal membrane oxygenation. paroxysm, a symptom of the infection of bordetella pertussis, was the cause of emphysema. in patients with severe neck subcutaneous emphysema, management of difficult airway is the most important safety issue in the practice of anesthesia. following the american society of anesthesiolo ... | 2014 | 24979862 |
| heterologous prime-boost immunization with live attenuated b. pertussis bpze1 followed by acellular pertussis vaccine in mice. | pertussis is a severe and life-threatening infectious disease. two successive generations of vaccines have strongly reduced its incidence over the last 70 years. however, despite excellent global vaccine coverage, it is still not under control and constitutes today the most frequent vaccine-preventable childhood disease. new vaccination approaches are therefore needed. here, we provide preclinical proof of concept for a heterologous prime-boost strategy, using the live attenuated bordetella pert ... | 2014 | 24950361 |
| [effect of yishen daluo decoction on the expression of plp, olig1, and olig2 in mice with experimental autoimmune encephalomyelitis]. | to study the effect of yishen daluo decoction (ydd) on the expression of protein lipoprotein (plp), oligodendrocyte transcription factor 1 (olig 1), and oligodendrocyte transcription factor 2 (olig2) in mice with experimental autoimmune encephalomyelitis (eae). | 2014 | 24941845 |