| effects of lactoferricin b against keratitis-associated fungal biofilms. | biofilms are considered as the most important developmental characteristics in ocular infections. biofilm eradication is a major challenge today to overcome the incidence of drug resistance. this report demonstrates the in vitro ability of biofilm formation on contact lens by three common keratitis-associated fungal pathogens, namely, aspergillus fumigatus, fusarium solani, and candida albicans. antifungal sensitivity testing performed for both planktonic cells and biofilm revealed the sessile p ... | 2012 | 22410856 |
| aspergillus fumigatus fungus ball in the pleural cavity. | to report the cases of 6 patients with fungus ball caused by aspergillus fumigatus (aspergilloma) in the pleural cavity. | 2016 | 22407049 |
| immune responses in cystic fibrosis: are they intrinsically defective? | cystic fibrosis (cf), the most common lethal single-gene disorder affecting northern europeans and north americans, is caused by mutations in the cystic fibrosis transmembrane conductance regulator (cftr) gene. cftr is a chloride channel and a regulator of other ion channels, and many aspects of the cf phenotype are directly related to ion channel abnormalities attributable to cftr mutation. lung disease is the most common limitation to the quantity and quality of life for patients with cf. one ... | 2012 | 22403802 |
| genome mining reveals the presence of a conserved gene cluster for the biosynthesis of ergot alkaloid precursors in the fungal family arthrodermataceae. | genome sequence analysis of different fungi of the family arthrodermataceae revealed the presence of a gene cluster consisting of five genes with high sequence similarity to those involved in the early common steps of ergot alkaloid biosynthesis in aspergillus fumigatus and claviceps purpurea. to provide evidence that this cluster is involved in ergot alkaloid biosynthesis, the gene arb_04646 of the fungus arthroderma benhamiae was cloned into pqe60 and expressed in escherichia coli. enzyme assa ... | 2012 | 22403186 |
| triazole fungicides can induce cross-resistance to medical triazoles in aspergillus fumigatus. | azoles play an important role in the management of aspergillus diseases. azole resistance is an emerging global problem in aspergillus fumigatus, and may develop through patient therapy. in addition, an environmental route of resistance development has been suggested through exposure to 14α-demethylase inhibitors (dmis). the main resistance mechanism associated with this putative fungicide-driven route is a combination of alterations in the cyp51a-gene (tr(34)/l98h). we investigated if tr(34)/l9 ... | 2012 | 22396740 |
| role of nod2 in regulating the immune response to aspergillus fumigatus. | the nucleotide-binding oligomerization domain-2 (nod2) is a type of intracellular receptor recognizing the cell wall molecules of bacteria and inducing the innate immune response. its role in defense against fungal infection remains uncertain. we thus investigated the role of the nod2/rip2 pathway in host responses to aspergillus fumigatus (af) in raw264.7 cells. | 2012 | 22395788 |
| neutrophils mediate maturation and efflux of lung dendritic cells in response to aspergillus fumigatus germ tubes. | invasive aspergillosis is a life-threatening complication of neutrophil deficiency or dysfunction. neutropenia has previously been associated with enhanced influx of cd11b-expressing conventional dendritic cells to the lungs in response to aspergillus species, but whether neutrophils directly modulate the function of dendritic cells in this infection is not known. we hypothesized that, in the setting of intrapulmonary challenge with aspergillus, neutrophils promote the maturation and traffic of ... | 2012 | 22392929 |
| the composition of the culture medium influences the β-1,3-glucan metabolism of aspergillus fumigatus and the antifungal activity of inhibitors of β-1,3-glucan synthesis. | in vitro testing of aspergillus fumigatus susceptibility to echinocandins has always been a challenge. using a simple and quick colorimetric method to analyze the activity of inhibitors of β-1,3-glucan synthesis, we found that the composition of the culture medium significantly influences glucan synthesis and consequently the antifungal properties of inhibitors of β-1,3-glucan synthesis when they are tested alone or in combination with chitin synthase inhibitors. | 2012 | 22391552 |
| host immune defense against aspergillus fumigatus: insight from experimental systemic (disseminated) infection. | model of systemic aspergillus fumigatus infection induced by intravenous application of conidia is suitable for studying important aspects of invasive aspergillosis including relationship between infection and mortality, dissemination of infection and immune mechanisms involved in host resistance to this fungus. use of this model allows the investigation of both innate and adaptive immune response characteristics in resistant/susceptible host, and investigating the contribution of genetic backgr ... | 2012 | 22388638 |
| the performance of real-time pcr, galactomannan, and fungal culture in the diagnosis of invasive aspergillosis in ventilated patients with chronic obstructive pulmonary disease (copd). | emerging reports have associated chronic pulmonary obstructive disease (copd) with invasive aspergillosis (ia), particularly in patients treated with mechanical ventilation and/or corticosteroids. this is a multicentre study in which copd patients demonstrating a new lung infiltrate while being mechanically ventilated were prospectively evaluated for the presence of ia. from the 47 patients studied, aspergillus fumigatus was recovered in culture in two patients (4.2%). while serum galactomannan ... | 2012 | 22382738 |
| antimicrobial polyacetylenes from panax ginseng hairy root culture. | two new polyacetylenes, 1-hydroxydihydropanaxacol (3) and 17-hydroxypanaxacol (4), were isolated from panax ginseng hairy root culture, along with dihydropanaxacol (1), panaxacol (2) and ginsenoyne d (5). highly hydroxylated compounds 1-4 were isolated from the medium and compound 5, which was a biosynthetic precursor of compound 1, was isolated from the roots. compounds 1-4 showed antimicrobial activity against staphylococcus aureus, bacillus subtilis, cryptococcus neoformans and aspergillus fu ... | 2012 | 22382419 |
| voriconazole and posaconazole improve asthma severity in allergic bronchopulmonary aspergillosis and severe asthma with fungal sensitization. | rationale and objectives. severe asthma with fungal sensitization (safs) and allergic bronchopulmonary aspergillosis (abpa) are progressive allergic fungal lung diseases whose effective treatment remains to be established. current treatment with itraconazole is associated with a 40% failure rate and adverse events (aes). we assessed the effect of voriconazole or posaconazole as second- and third-line therapies. methods. we conducted a retrospective review of adult asthmatic patients with either ... | 2012 | 22380765 |
| aspergillus endocarditis: a case of near complete left ventricular outflow obstruction. | a 60-year old woman presented with dyspnoea and fatigue. she was frail and cachectic (bmi 17.5) with a pancytopenia. previously she had received chemotherapy for chronic lymphatic leukaemia. she relapsed one year ago necessitating a reduced intensity conditioning allogeneic haematopoietic cell transplantation. subsequently, graft versus host disease required high-dose immunosuppressants. computerized tomography on admission showed bilateral lung nodules and a suspicious cardiac mass. bronchial b ... | 2012 | 22374293 |
| gliotoxin contamination in and pre- and postfermented corn, sorghum and wet brewer's grains silage in sao paulo and rio de janeiro state, brazil. | the aim of this study was to determine total fungal counts and the relative density of aspergillus fumigatus and related species in silage samples intended for bovines before and after fermentation as well as to monitor the natural occurrence of gliotoxin in silage samples (pre- and postfermentation). | 2012 | 22372472 |
| dige enables the detection of a putative serum biomarker of fungal origin in a mouse model of invasive aspergillosis. | invasive aspergillosis (ia) is a major threat for immunocompromised patients. diagnostic difficulties often delay specific treatment initiation, which increases mortality. finding new biomarkers to improve and speed accurate diagnosis is thus vital. to investigate the ability of proteomic methods for discovering new biomarkers of ia, we used a dige approach to perform a proteomic analysis on both bronchoalveolar lavages (bal) and sera at different time-points of infection in a mouse model of inv ... | 2012 | 22370163 |
| developments in novel breath tests for bacterial and fungal pulmonary infection. | breath testing has developed over the last 20 years. new techniques that can identify fingerprints for specific diseases and specific markers of respiratory pathogens have been applied to breath analysis. this review discusses the recent advances in breath analysis for the diagnosis of bacterial and fungal lower respiratory tract infections. | 2012 | 22366996 |
| antibody specific to thioredoxin reductase as a new biomarker for serodiagnosis of invasive aspergillosis in non-neutropenic patients. | invasive aspergillosis (ia) is an important cause of mortality in critically ill patients, but the diagnosis is difficult as clinical and radiological signs are neither sensitive nor specific. serum galactomannan (gm) is a useful marker for ia, but exhibits low sensitivity in non-neutropenic patients. in our previous work, strong antibody reactivity to thioredoxin reductase of aspergillus fumigatus was found in non-neutropenic ia patients. | 2012 | 22366166 |
| an unusual case of non-disseminated bladder aspergillosis in a setting of transitional cell carcinoma. | a bladder infection of aspergillus with no evidence of dissemination is rare. we present a case of aspergillus infection with transitional cell carcinoma of the urinary bladder without any evidence of systemic involvement. a 65-year-old male diabetic whose main complaints were intermittent painful haematuria and nocturia had undergone nephroureterectomy a year and a half back for transitional cell carcinoma of right renal pelvis. cystoscopy revealed bladder mucosa having fixed broad tumour with ... | 2012 | 22361774 |
| secretome of human bronchial epithelial cells in response to the fungal pathogen aspergillus fumigatus analyzed by differential in-gel electrophoresis. | for years, the analysis of innate responses to the major mold pathogen aspergillus fumigatus has been restricted to specialized cells, such as professional phagocytes. more recently, the contribution of the airway epithelial barrier has been assessed and studies have shown that it was able to sense and react to the aspergillus infection, for example, by producing cytokines. | 2012 | 22357658 |
| sensing of mammalian il-17a regulates fungal adaptation and virulence. | infections by opportunistic fungi have traditionally been viewed as the gross result of a pathogenic automatism, which makes a weakened host more vulnerable to microbial insults. however, fungal sensing of a host's immune environment might render this process more elaborate than previously appreciated. here we show that interleukin (il)-17a binds fungal cells, thus tackling both sides of the host-pathogen interaction in experimental settings of host colonization and/or chronic infection. global ... | 2012 | 22353714 |
| clinical characteristics and prognosis of chronic pulmonary aspergillosis. | the details of the clinical characteristics of patients with chronic pulmonary aspergillosis (cpa) have not been fully understood. | 2012 | 22349065 |
| total synthesis and structure-activity relationships of new echinocandin-like antifungal cyclolipohexapeptides. | a series of new echinocandin-like cyclolipohexapeptides were designed and total synthesized via solution phase [3 + 3]-segment coupling strategy with an attempt to improve antifungal activity. the designed compounds showed potent antifungal activities with broad spectrum. in particular, 11 compounds (i.e. 28a-e, 28g, 28i-j, 29a, 29c and 29e) showed better in vitro antifungal activities against candida albicans or aspergillus fumigatus than caspofungin. moreover, the synthesized compounds provide ... | 2012 | 22348827 |
| iron - a key nexus in the virulence of aspergillus fumigatus. | iron is an essential but, in excess, toxic nutrient. therefore, fungi evolved fine-tuned mechanisms for uptake and storage of iron, such as the production of siderophores (low-molecular mass iron-specific chelators). in aspergillus fumigatus, iron starvation causes extensive transcriptional remodeling involving two central transcription factors, which are interconnected in a negative transcriptional feed-back loop: the gata-factor srea and the bzip-factor hapx. during iron sufficiency, srea repr ... | 2012 | 22347220 |
| molecular characterization of the putative transcription factor seba involved in virulence in aspergillus fumigatus. | aspergillus fumigatus is a major opportunistic pathogen and allergen of mammals. nutrient sensing and acquisition mechanisms, as well as the capability to cope with different stressing conditions, are essential for a. fumigatus virulence and survival in the mammalian host. this study characterized the a. fumigatus seba transcription factor, which is the putative homologue of the factor encoded by trichoderma atroviride seb1. the δseba mutant demonstrated reduced growth in the presence of paraqua ... | 2012 | 22345349 |
| 2,5-diketopiperazines from the marine-derived fungus aspergillus fumigatus yk-7. | five new diketopiperazines, prenylcyclotryprostatin b (1), 20-hydroxycyclotryprostatin b (2), 9-hydroxyfumitremorgin c (3), 6-hydroxytryprostatin b (4), and spirogliotoxin (5), were isolated from the marine-derived fungus aspergillus fumigatus yk-7, along with nine known compounds, 6-14. their structures were elucidated by spectroscopic methods, and their antiproliferative effects on human leukemic monocyte lymphoma u937 and human prostate cancer pc-3 cell lines were assessed in vitro. compounds ... | 2012 | 22344914 |
| nonribosomal peptide synthetase genes pesl and pes1 are essential for fumigaclavine c production in aspergillus fumigatus. | the identity of metabolites encoded by the majority of nonribosomal peptide synthetases in the opportunistic pathogen, aspergillus fumigatus, remains outstanding. we found that the nonribosomal peptide (nrp) synthetases pesl and pes1 were essential for fumigaclavine c biosynthesis, the end product of the complex ergot alkaloid (ea) pathway in a. fumigatus. deletion of either pesl (δpesl) or pes1 (δpes1) resulted in complete loss of fumigaclavine c biosynthesis, relatively increased production of ... | 2012 | 22344643 |
| risk factors for mycobacterium abscessus infection in cystic fibrosis patients; a case-control study. | mycobacterium abscessus is a nontuberculous mycobacterium that is increasingly recognized as an opportunistic pathogen in cystic fibrosis (cf) patients. factors that predispose cf patients to infection by this environmental bacterium remain unknown. in a case-control study of 22 cf patients with m. abscessus infection and 22 cf controls, we investigated risk factors for mab infection as well as the positioning of mab infection in relation to the other pathogens infecting cf patients. no clear ri ... | 2012 | 22342403 |
| [aspergillosis. clinical forms and treatment]. | invasive aspergillosis, chronic pulmonary aspergillosis and allergic bronchopulmonary aspergillosis are the clinical forms of aspergillosis. although there is a great number of aspergillus species, aspergillus fumigatus-complex is the more frequent aetiological agent, regardless of clinical form or baseline condition. the increase in immunosuppressive agents and the higher use of corticosteroids in chronic obstructive pulmonary disease have led to aspergillosis becoming more prominent in recent ... | 2012 | 22341751 |
| characterization of a thermostable β-glucosidase from aspergillus fumigatus z5, and its functional expression in pichia pastoris x33. | recently, the increased demand of energy has strongly stimulated the research on the conversion of lignocellulosic biomass into reducing sugars for the subsequent production, and β-glucosidases have been the focus because of their important roles in a variety fundamental biological processes and the synthesis of useful β-glucosides. although the β-glucosidases of different sources have been investigated, the amount of β-glucosidases are insufficient for effective conversion of cellulose. the goa ... | 2012 | 22340848 |
| structural insight into the unique substrate binding mechanism and flavin redox state of udp-galactopyranose mutase from aspergillus fumigatus. | udp-galactopyranose mutase (ugm) is a flavin-containing enzyme that catalyzes the reversible conversion of udp-galactopyranose (udp-galp) to udp-galactofuranose (udp-galf). as in prokaryotic ugms, the flavin needs to be reduced for the enzyme to be active. here we present the first eukaryotic ugm structures from aspergillus fumigatus (afugm). the structures are of ugm alone, with the substrate udp-galp and with the inhibitor udp. additionally, we report the structures of afugm bound to substrate ... | 2012 | 22334662 |
| epidemiological cutoff values for azoles and aspergillus fumigatus based on a novel mathematical approach incorporating cyp51a sequence analysis. | epidemiological cutoff values (ecv) are commonly used to separate wild-type isolates from isolates with reduced susceptibility to antifungal drugs, thus setting the foundation for establishing clinical breakpoints for aspergillus fumigatus. however, ecvs are usually determined by eye, a method which lacks objectivity, sensitivity, and statistical robustness and may be difficult, in particular, for extended and complex mic distributions. we therefore describe and evaluate a statistical method of ... | 2012 | 22330922 |
| keratomycosis caused by aspergillus viridinutans: an aspergillus fumigatus-resembling mold presenting distinct clinical and antifungal susceptibility patterns. | we report here a case of fungal keratitis caused by aspergillus viridinutans, a member of the genus which was found to have clinical and antifungal susceptibility characteristics distinct from the species it most closely resembles, aspergillus fumigatus. a 26-year-old woman presented with contact lens-associated keratitis, the etiologic agent of which was initially slow growing and found to be resistant to amphotericin b and voriconazole. therapeutic keratoplasty was performed and an isolate fro ... | 2012 | 22329455 |
| a nebulized intra-tracheal rat model of invasive pulmonary aspergillosis. | animal models are particularly useful for the study of many infectious diseases, including those caused by fungi. invasive pulmonary aspergillosis is most frequently studied in mouse models. we present here an animal model of this disease based on undernourished immunocompromised rats infected with aspergillus fumigatus spores by intra-tracheal nebulisation. | 2012 | 22328399 |
| embryonated chicken eggs as alternative infection model for pathogenic fungi. | embryonated eggs have been used as infection models for decades in virology and bacteriology. however, they can also be used as an attractive alternative infection model for studying fungal pathogenesis. here, we discuss some general aspects which need to be considered when working with embryonated eggs as infection models. furthermore, we provide detailed protocols and technical tips for infection of embryonated eggs with aspergillus fumigatus and candida albicans via the chorioallantois membra ... | 2012 | 22328397 |
| simple assays for measuring innate interactions with fungi. | in recent decades, there has been a steady rise in immunocompromised populations and consequently a dramatic increase in the clinical relevance of normally non-pathogenic and commensal fungi such as aspergillus fumigatus and candida albicans. understanding how these fungi interact with the host immune system is important for the development of immunotherapeutic approaches. here, we describe a number of methods which have been developed to investigate the interactions of fungi with host leukocyte ... | 2012 | 22328383 |
| targeted gene deletion in aspergillus fumigatus using the hygromycin-resistance split-marker approach. | the construction of a fungal strain that lacks a specific gene product is often accomplished by replacing the gene of interest with a selection marker using site-specific recombination. transformation of aspergillus fumigatus, like many related fungal species, must overcome two major obstacles. first, the cell wall limits the entry of exogenous dna, and second, a high rate of nonhomologous recombination leads to random ectopic integration of the marker. here, we describe an experimental strategy ... | 2012 | 22328371 |
| gene disruption in aspergillus fumigatus using a pcr-based strategy and in vivo recombination in yeast. | aspergillus fumigatus is a ubiquitous, filamentous fungal saprophyte and is the causative agent of the vast majority of aspergillosis in that invasive aspergillosis is the life-threatening form of infection by this fungus. the study of gene function using null mutants in this organism can be achieved through dna-mediated transformation with an engineered deletion cassette containing about 2 kb of the 5'- and 3'-flanking region of the target gene and a selectable marker. here, we describe the use ... | 2012 | 22328370 |
| production of thermophilic endo-β-1,4-xylanases by aspergillus fumigatus fbspe-05 using agro-industrial by-products. | in the present paper, endo-β-1,4-xylanase production by aspergillus fumigatus was evaluated in solid-state fermentation using low-cost substrates such as sugarcane bagasse (scb), brewer's spent grain (bsg), and wheat bran (wb). the partial characterization of the crude enzyme was also performed. in the experimental conditions, the highest levels of endo-β-1,4-xylanase production by a. fumigatus fbspe-05 occurred within 8 days incubation when using scb/liquid medium at 1:2 ratio (219.5 u g(-1)) a ... | 2012 | 22328248 |
| persistence versus escape: aspergillus terreus and aspergillus fumigatus employ different strategies during interactions with macrophages. | invasive bronchopulmonary aspergillosis (ibpa) is a life-threatening disease in immunocompromised patients. although aspergillus terreus is frequently found in the environment, a. fumigatus is by far the main cause of ibpa. however, once a. terreus establishes infection in the host, disease is as fatal as a. fumigatus infections. thus, we hypothesized that the initial steps of disease establishment might be fundamentally different between these two species. since alveolar macrophages represent o ... | 2012 | 22319619 |
| trypacidin, a spore-borne toxin from aspergillus fumigatus, is cytotoxic to lung cells. | inhalation of aspergillus fumigatus conidia can cause severe aspergillosis in immunosuppressed people. a. fumigatus produces a large number of secondary metabolites, some of which are airborne by conidia and whose toxicity to the respiratory tract has not been investigated. we found that spores of a. fumigatus contain five main compounds, tryptoquivaline f, fumiquinazoline c, questin, monomethylsulochrin and trypacidin. fractionation of culture extracts using rp-hplc and lc-ms showed that sample ... | 2012 | 22319557 |
| species-specific recognition of aspergillus fumigatus by toll-like receptor 1 and toll-like receptor 6. | aspergillus fumigatus causes invasive aspergillosis, a potentially fatal infection in oncohematological patients. innate immune detection of a. fumigatus involves toll-like receptor (tlr) 4 and tlr2, which forms a heterodimer with either tlr1 or tlr6. the role of those coreceptors in aspergillus sensing is unknown. | 2012 | 22315281 |
| expression turnover profiling to monitor the antifungal activities of amphotericin b, voriconazole, and micafungin against aspergillus fumigatus. | eight highly expressed candidate genes were selected for mrna profiling to monitor the transcriptome kinetics of aspergillus fumigatus strains exposed to antifungal drugs as potential biomarkers of live cells to assess treatment efficacy. mycelia were treated with fungicidal drugs amphotericin b and voriconazole, as well as the fungistatic drug micafungin. transcription was monitored at 0, 4, 8, and 24 h posttreatment. the expression turnover profile provides a possible tool to assess antifungal ... | 2012 | 22314535 |
| assessment of aspergillus fumigatus in guinea pig bronchoalveolar lavages and pulmonary tissue by culture and realtime polymerase chain reaction studies. | in this study we pursued a diagnostic target in aspergillus fumigatus (af) by using qualitative realtime pcr combined with proprietary dna primers and a hydrolysis probe specific for this fungal target. qualitative realtime pcr is a diagnostic tool that utilizes realtime pcr technology and detects the presence or absence target specific dna within a predetermined detection range. respiratory tissue and fluids from experimentally infected guinea pigs were tested by extracting dna from the samples ... | 2012 | 22312282 |
| a comparative study of hydrolysis and transglycosylation activities of fungal β-glucosidases. | β-glucosidases (bgs) from aspergillus fumigatus, aspergillus niger, aspergillus oryzae, magnaporthe grisea, neurospora crassa, and penicillium brasilianum were purified to homogeneity, and investigated for their (simultaneous) hydrolytic and transglycosylation activity in samples with high concentrations of either cellobiose or glucose. the rate of the hydrolytic process (which converts one cellobiose to two glucose molecules) shows a maximum around 10-15 mm cellobiose and decreases with further ... | 2013 | 22311644 |
| transcriptomic and proteomic analyses of the aspergillus fumigatus hypoxia response using an oxygen-controlled fermenter. | aspergillus fumigatus is a mold responsible for the majority of cases of aspergillosis in humans. to survive in the human body, a. fumigatus must adapt to microenvironments that are often characterized by low nutrient and oxygen availability. recent research suggests that the ability of a. fumigatus and other pathogenic fungi to adapt to hypoxia contributes to their virulence. however, molecular mechanisms of a. fumigatus hypoxia adaptation are poorly understood. thus, to better understand how a ... | 2012 | 22309491 |
| current understanding of ptx3 protective activity on aspergillus fumigatus infection. | infection caused by aspergillus fumigatus remains a major therapeutic challenge in immunocompromised individuals. innate immunity represents the first line of defense against pathogens. in the last 20 years, several proteins belonging to this arm of the immune system have been characterized as being endowed with antifungal activity. among these, the prototype long pentraxin ptx3 has been identified as a non-redundant protective factor against infections caused by a. fumigatus. a number of releva ... | 2012 | 22309253 |
| a novel dehydrin-like protein from aspergillus fumigatus regulates freezing tolerance. | we recently reported on dpra and dprb, dehydrin-like proteins involved in the protection against oxidative, osmotic and ph stress in aspergillus fumigatus. a third dehydrin-like gene, dprc, was detected in the genome of the filamentous fungus and repressed during conidial germination. the deletion mutant was impaired in freezing tolerance. the dprc-mediated response was dependent on the saka map kinase pathway, and accordingly, saka phosphorylation was induced by cold shock. when fused to egfp, ... | 2012 | 22306917 |
| trichostatin a abrogates airway constriction, but not inflammation, in murine and human asthma models. | histone deacetylase (hdac) inhibitors may offer novel approaches in the treatment of asthma. we postulate that trichostatin a (tsa), a class 1 and 2 inhibitor of hdac, inhibits airway hyperresponsiveness in antigen-challenged mice. mice were sensitized and challenged with aspergillus fumigatus antigen (af) and treated with tsa, dexamethasone, or vehicle. lung resistance (r(l)) and dynamic compliance were measured, and bronchial alveolar lavage fluid (balf) was analyzed for numbers of leukocytes ... | 2012 | 22298527 |
| [triple fungal infection in a patient with liver cirrhosis]. | the prevalence of invasive mycoses is increasing, especially among patients who are immunocompromised or hospitalized with serious underlying diseases. such infections are associated with a high morbidity and significant mortality, requiring early diagnosis and appropriate treatment but also an optimal prophylaxis in patients with high risk factors. we report a case of triple fungal infection including an invasive pulmonary aspergillosis by aspergillus fumigatus, a candidemia by candida albicans ... | 2012 | 22294141 |
| fetuin a, a serum component, promotes growth and biofilm formation by aspergillus fumigatus. | aspergillus fumigatus is an all-important pathogenic fungus and is known for its angiotropism. when it invades human organs, a. fumigatus makes direct contact with blood and its components by causing inflammation and invading vascular structures. to learn the effect of its contact with blood on the development of infection, we examined the effect of serum on a. fumigatus growth. in dulbecco's modified eagle's medium containing 10% fetal bovine serum, hyphal tip growth was accelerated, forming a ... | 2012 | 22293034 |
| nce102 homologue in aspergillus fumigatus is required for normal sporulation, not hyphal growth or pathogenesis. | in saccharomyces cerevisiae, nce102 encodes a 173 amino acid transmembrane protein, which acts as a key player in eisosome assembly and plasma membrane organization. here, we describe the characterization of nce102 homologue in the human pathogen, aspergillus fumigatus. our results demonstrated that afunce102 is continuously expressed during fungal growth. in addition, microscopic examination of an afunce102-gfp-expressing transformant confirmed the localization of the fusion protein to the endo ... | 2012 | 22289033 |
| duality of liver and kidney lesions after systemic infection of immunosuppressed and immunocompetent mice with aspergillus fumigatus. | invasive aspergillosis is a life-threatening disease mainly caused by aspergillus fumigatus. patients at risk are generally immunocompromised and lungs are assumed to provide the primary site for infection and invasive disease manifestation. contrarily, visceral organ involvement appears to result from a subsequent hematogenous spread. to compare the kinetics of dissemination within deep organs in immunosuppressed vs. immunocompetent mice, we used a bioluminescent a. fumigatus strain in an intra ... | 2012 | 22286702 |
| surface display of gaussia princeps luciferase allows sensitive fungal pathogen detection during cutaneous aspergillosis. | non-invasive imaging techniques in microbial disease models have delivered valuable insights in the intimate pathogen-host interplay during infection. here we describe evaluation and validation of a transgenic bioluminescence reporter strain of the human-pathogenic mold aspergillus fumigatus, one of the main fungal pathogens affecting immunocompromised individuals. expression and surface display of the gaussia princeps luciferase allowed sensitive and rapid detection of luminescence emitted from ... | 2012 | 22286700 |
| shedding natural light on fungal infections. | bioluminescence imaging allows the visualization of the temporal and spatial progression of biological phenomena, in particular infection, by non-invasive methods in vivo. this nature-borrowed technology has been successfully used to monitor bacterial infections but recent studies have also succeeded in tracking fungal infections such as those caused by the two major opportunistic fungal pathogens candida albicans and aspergillus fumigatus. the findings of donat and collaborators published in th ... | 2012 | 22286695 |
| functional analysis is a paramount prerequisite for understanding the in vitro interaction of human natural killer cells with aspergillus fumigatus. | | 2012 | 22279122 |
| hypersensitivity testing for aspergillus fumigatus ige is significantly more sensitive than testing for aspergillus niger ige. | we sought to determine if sufficient redundancy exists between specific ige testing for aspergillus fumigatus and aspergillus niger to eliminate one of the assays in determining aspergillus hypersensitivity. we reviewed regional laboratory results comparing a fumigatus-specific ige with a niger-specific ige using the pharmacia unicap system (pharmacia, kalamazoo, mi). by using the fisher exact test as an index of concordance among paired results, we showed a significant difference between 109 pa ... | 2012 | 22261444 |
| small surfactant-like peptides can drive soluble proteins into active aggregates. | inactive protein inclusion bodies occur commonly in escherichia coli (e. coli) cells expressing heterologous proteins. previously several independent groups have found that active protein aggregates or pseudo inclusion bodies can be induced by a fusion partner such as a cellulose binding domain from clostridium cellulovorans (cbdclos) when expressed in e. coli. more recently we further showed that a short amphipathic helical octadecapeptide 18a (ewlkafyekvleklkelf) and a short beta structure pep ... | 2012 | 22251949 |
| interlaboratory comparison of pcr-based identification of candida and aspergillus dna in spiked blood samples. | despite pcr per se being a powerful and sensitive technique, regarding the detection of fungi in patients' blood, no consensus for a standardised pcr protocol yet exists. to complement other ongoing or accomplished studies which tackle this problem, the german reference center for systemic mycoses conducted an interlaboratory comparison starting with blood samples spiked with fungal cell elements. altogether, six laboratories using in-house pcr-protocols from germany and austria participated in ... | 2012 | 22248125 |
| outbreak of invasive aspergillosis after major heart surgery caused by spores in the air of the intensive care unit. | outbreaks of invasive aspergillosis (ia) are believed to be caused by airborne aspergillus conidia. few studies have established a correlation between high levels of aspergillus fumigatus conidia and the appearance of new cases of ia or have demonstrated matching genotypes between clinical isolates and those from the environment. | 2012 | 22247307 |
| resistin-like molecule-α regulates il-13-induced chemokine production but not allergen-induced airway responses. | resistin-like molecule α (relm-α) is one of the most up-regulated gene products in allergen- and parasite-associated th2 responses. localized to alternatively activated macrophages, relm-α was shown to exert an anti-inflammatory effect in parasite-induced th2 responses, but its role in experimental asthma remains unexplored. here, we analyzed the cellular source, the il-4 receptors required to stimulate relm-α production, and the role of relm-α after experimental asthma induction by il-4, il-13, ... | 2012 | 22246861 |
| trivalent ultrashort lipopeptides are potent ph dependent antifungal agents. | the activity of antimicrobial peptides (amps) that contain a large proportion of histidine residues (pk(a) ∼ 6) depends on the physiological ph environment. advantages of these amps include high activity in slightly acidic areas of the human body and relatively low toxicity in other areas. also, many amps are highly active in a multivalent form, but this often increases toxicity. here we designed ph dependent amphiphilic compounds consisting of multiple ultrashort histidine lipopeptides on a tri ... | 2012 | 22243686 |
| prophylactic efficacy of single dose pulmonary administration of amphotericin b inhalation powder in a guinea pig model of invasive pulmonary aspergillosis. | amphotericin b inhalation powder (abip) is a novel dry-powder amphotericin b formulation that is directly delivered to the lung, resulting in elevated lung tissue drug concentrations of this polyene. we evaluated the prophylactic efficacy of single dose administration of abip in a guinea pig model of invasive pulmonary aspergillosis. | 2012 | 22240402 |
| ctbt (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) producing ros affects growth and viability of filamentous fungi. | ctbt (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) causes intracellular superoxide production and oxidative stress and enhances the susceptibility of saccharomyces cerevisiae, candida albicans, and c. glabrata cells to cycloheximide, 5-fluorocytosine, and azole antimycotic drugs. here, we demonstrate the antifungal activity of ctbt against 14 tested filamentous fungi. ctbt prevented spore germination and mycelial proliferation of aspergillus niger and the pathogenic aspergillus fumigatus. the a ... | 2012 | 22212016 |
| antifungal drug resistance: mechanisms, epidemiology, and consequences for treatment. | antifungal resistance continues to grow and evolve and complicate patient management, despite the introduction of new antifungal agents. in vitro susceptibility testing is often used to select agents with likely activity for a given infection, but perhaps its most important use is in identifying agents that will not work, i.e., to detect resistance. standardized methods for reliable in vitro antifungal susceptibility testing are now available from the clinical and laboratory standards institute ... | 2012 | 22196207 |
| invasive fungal infections after renal transplantation. | invasive fungal infection (ifi) is a leading cause of infection-related mortality among kidney allograft recipients. | 2012 | 25013619 |
| ras-mediated signal transduction and virulence in human pathogenic fungi. | signal transduction pathways regulating growth and stress responses are areas of significant study in the effort to delineate pathogenic mechanisms of fungi. in-depth knowledge of signal transduction events deepens our understanding of how a fungal pathogen is able to sense changes in the environment and respond accordingly by modulation of gene expression and re-organization of cellular activities to optimize fitness. members of the ras protein family are important regulators of growth and diff ... | 2012 | 24855584 |
| fulminant antiphospholipid antibody syndrome complicated by aspergillus tracheobronchitis. | aspergillus fumigatus is a filamentous mold that causes infections in patients who are inmmunocompromised. we report a case of aspergillus tracheobronchitis in fulminant systemic lupus erythematosus case. diagnosis with more invasive diagnostic procedures & aggressive antifungal therapy is indicated at early stage. | 2012 | 24371751 |
| early aspergillus pacemaker pocket infection: case and review. | we report the first case to our knowledge of an early pacemaker pocket infection due to aspergillus fumigatus. several cases of late pacemaker pocket infection by aspergillus have been reported, but it remains exceedingly rare. recognition of aspergillus infection as a potential early or late complication of placement of pacemakers or implantable cardioverter defibrillator devices may help clinicians diagnose and treat future cases of this potentially devastating infection. | 2012 | 24371732 |
| allergic bronchopulmonary mycosis due to alternaria: case report and review. | while allergic bronchopulmonary aspergillosis and mycosis are well recognised, no cases have been described related to alternaria spp. alternaria is a common sensitising fungus in asthmatics and related to thunderstorm asthma. we report a case of an asthmatic who presented with worsening asthma control, mild eosinophilia on high dose inhaled corticosteroids (800 μg/day), a total ige of 3800 kiu/l, an alternaria-specific ige of 21.3 kua/l and positive skin prick test, negative specific ige and sk ... | 2012 | 24371728 |
| aspergillus osteomyelitis of the lumbar spine complicated with orbital apex syndrome: a potential role of the batson's plexus in disease propagation. | we report a rare case of orbital apex syndrome following epidural steroid injections of the lumbar spine in an immunocompetent individual with osteomyelitis and discitis caused by aspergillus fumigatus. we suspect that the craniospinal venous system, also known as the batson's plexus, was the main route for steroid-facilitated disease propagation from the spine to intracranial structures. | 2012 | 24371725 |
| the prevalence of positive fungal cultures in patients with chronic rhinosinusitis in a high altitude region of iran. | there are suspended fungal spores in the air and in the nasal mucosa of adults, especially in areas with a humid climate. several studies have revealed the role of fungi in the pathogenesis of chronic rhinosinusitis (crs) in recent years but it is a topic of controversy, especially in regions with low humidity. the aim of this study was to evaluate the prevalence of fungal species in intraoperative specimens from patients who underwent functional endoscopic sinus surgery (fess) for crs in hamada ... | 2012 | 24303382 |
| candida albicans: a model organism for studying fungal pathogens. | candida albicans is an opportunistic human fungal pathogen that causes candidiasis. as healthcare has been improved worldwide, the number of immunocompromised patients has been increased to a greater extent and they are highly susceptible to various pathogenic microbes and c. albicans has been prominent among the fungal pathogens. the complete genome sequence of this pathogen is now available and has been extremely useful for the identification of repertoire of genes present in this pathogen. th ... | 2012 | 23762753 |
| concentrations and diversity of microbes from four local bioaerosol emission sources in finland. | microbial particles can readily be released into the air from different types of man-made sources such as waste operations. microbiological emissions from different biological sources and their dispersion may be an issue of concern for area planning and for nearby residents. this study was designed to determine the concentrations and diversity of microbiological emissions from four different man-made source environments: waste center with composting windrows, sewage treatment plant, farming envi ... | 2011 | 28097955 |
| synthesis, characterization, and antimicrobial evaluation of carbostyril derivatives of 1h-pyrazole. | a new series of 12 derivatives of 4-pyrazolyl-n-arylquinoline-2,5-dione (4a-l) were synthesized by one pot base catalyzed cyclocondensation reaction of 1-aryl-5-chloro-3-methyl-1h-pyrazole-4-carbaldehyde (1a-c), meldrum's acid (2) and 3-arylamino-5,5-disubstitutedcyclohex-2-enone (3a-d). all the compounds were characterized by elemental analysis, ft-ir, (1)h nmr and (13)c nmr spectral data and were screened, against six bacterial pathogens, namely bacillus subtilis, clostridium tetani, streptoco ... | 2011 | 23960745 |
| [primitive aspergillosis of the posterior cerebral fossa in immunocompetent patient]. | cerebral aspergillosis arises in the great majority of cases during an invasive aspergillosis with hematogene scattering from the lung hurts. the cerebral, not rare location is one of the worse criteria forecast during the invasive aspergillosis. we report the case of patient who was hospitalized in the neurosurgery department for syndrome of increased intracranial pressure, hemiparesis and cerebellar syndrome in febrile context. the radiological exploration objectified a collection of the poste ... | 2011 | 24451505 |
| the potential inhibitory effect of cuminum cyminum, ziziphora clinopodioides and nigella sativa essential oils on the growth of aspergillus fumigatus and aspergillus. | the goals of this study were to evaluate the effectiveness of cuminum cyminum, ziziphora clinopodioides and nigella sativa essential oils to inhibit the growth of aspergillus fumigatus and a. flavus and to evoke ultrastructural changes. the fungi were cultured into rpmi 1640 media in the presence of oils at concentrations of 8, 6, 5, 4, 3, 2, 1.5, 1.25, 1, 0.75 and 0.5 mg/ml in broth microdilution and 2, 1.5, 1 and 0.5 mg/ml in broth macrodilution methods with shaking for 48 h at 28(o)c. conidia ... | 2011 | 24031624 |
| identification of fungi associated with rotylenchulus reniformis. | the objective of this work was to isolate and identify fungi associated with r. reniformis in cotton roots. soil samples were collected in cotton fields naturally infested with r. reniformis and from cotton stock plants cultured in the greenhouse. nematodes extracted from the soil were observed under the stereoscope, and discolored eggs and vermiform stages colonized with mycelia were cultured on 1.5% water agar supplemented with antibiotics, and incubated at 27°c. identification of the nematoph ... | 2010 | 22736864 |
| prevalence of otomycosis in ibadan: a review of laboratory reports. | otomycosis has typically been described as fungal infection of the external auditory canal with infrequent complications involving the middle ear. because of the dearth of data on the prevalence of otomycosis in this environment and increased rate of immunosuppression, this study was aimed at finding the prevalence and fungal etiological agents responsible for this condition. aretrospective review of the laboratory reports of ear swabs of patients that presented with signs and symptoms suggestiv ... | 2010 | 22420052 |
| synthesis and evaluation of novel bis[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles as potent antimicrobial agents. | a series of novel bis[4-methoxy-3-(6-aryl[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol)phenyl]methanes 5a-l has been synthesized and characterized via ir, 1h nmr, 13c nmr, ms and elemental analyses. all the newly synthesized compounds were screened for their antibacterial activity against bacillus subtilis, bacillus sphaericu, staphylococcus aureus, pseudomonas aeruginosa, klobsinella aerogenes and chromobacterium violaceum and antifungal activity against candida albicans, aspergillus fumigatus, trich ... | 2010 | 24061822 |
| an investigation into the antifungal property of fabaceae using bioinformatics tools. | chemodiversity in plants provides sources of great value which might be helpful for finding new leads in drug discovery programs. fabaceae as the third largest family of flowering plants was chosen to investigate its possible antifungal activity. in order to increase the effectiveness of the result, molecular similarity methods and chemical data were used. twelve plants were selected from fabaceae and collected from the north and south of iran. percolation method with 80% ethanol was used for ex ... | 2010 | 23407751 |
| isolation and identification of aspergillus section fumigati strains from arable soil in korea. | 63 strains of aspergillus section fumigati were isolated from 17 samples of arable soil in a central province of korea. based on the results of genotypic and phenotypic analyses, they were identified as aspergillus fumigatus, a. lentulus, neosartorya coreana, n. fennelliae, n. fischeri, n. glabra, n. hiratsukae, n. laciniosa, n. pseudofischeri, n. quadricincta, n. spinosa and n. udagawae. among these, n. fennelliae, n. hiratsukae, n. quadricincta, and n. udagawae had not been previously recorded ... | 2010 | 23956617 |
| diversity and exploration of bioactive marine actinomycetes in the bay of bengal of the puducherry coast of india. | the present study was designed to investigate the puducherry coast of the bay of bengal, india for the diversity of bioactive actinomycetes. a total of 50 actinomycete strains were isolated from the marine sediments and most of the strains were belongs to streptomyces. these strains were identified by means of morphological physiological, biochemical and cultural characteristics. the isolates were subjected to shake flask fermentation and the secondary metabolites were extracted with ethyl aceta ... | 2010 | 23100811 |
| bilateral simultaneous endogenous aspergillus endophthalmitis in an immunocompetent patient. | to report a case of bilateral endogenous aspergillus endophthalmitis in an immunocompetent patient. | 2010 | 25390109 |
| opportunistic infection in the patients with acute liver failure: a report of three cases with one fatality. | we report three cases of severe opportunistic infection in patients with acute liver failure (alf). these cases included a 56-year-old man infected with cryptococcus neoformans, cytomegarovirus (cmv), candida albicans and aspergillus fumigates, as well as a 62-year-old man and a 54-year-old woman infected with pneumocystis pneumonia, cmv and aspergillus fumigatus. one patient died as a result of the infection rather than liver failure. we stress the importance of opportunistic infection as a con ... | 2009 | 26192799 |
| gliotoxin in aspergillus fumigatus: an example that mycotoxins are potential virulence factors. | moulds produce several different mycotoxins that may improve their chance of survival in particular environments. for example, aspergillus fumigatus, an important human pathogen, produces several mycotoxins including gliotoxin. this secondary metabolite, a small lipid soluble dipeptide, exerts toxic effects on phagocytic cells and t-lymphocytes at low concentrations in vitro. a. fumigatus also produces high levels of gliotoxin in vivo, and this suggests that host defense mechanisms might be impa ... | 2009 | 23605091 |
| expressed sequence tags of aspergillus fumigatus: extension of catalogue and their evaluation as putative drug targets and/or diagnostic markers. | aspergillus fumigatus a fungal pathogen is implicated in a spectrum of allergic and invasive disorders in humans. validation of transcriptome of pathogen is essential for understanding its virulence mechanism and to identify new therapeutic targets/diagnostic markers. in order to rapidly identify genes of aspergillus fumigatus we adopted sequencing of cdna clones. our earlier effort has lead to identification of 68 expressed sequence tags of aspergillus fumigatus. present study describes 52 more ... | 2009 | 23105821 |
| purulent thyroiditis-a clinical study of 5 cases. | between 1 january 1990 and 31 december 2006, 13279 patients were operated on due to various forms of goitre, of which five cases were diagnosed with purulent thyroiditis-one child aged 8, three women and one man all between 35 and 81 years of age. the course of the disease was unusual. in three of the patients cancer was suspected. in all of the patients hard nodules were discovered within the thyroid gland, ultrasonographically heterogeneous and hypo-echogenic, although scintigraphic examinatio ... | 2009 | 27377315 |
| invasive aspergillosis associated with systemic lupus erythematosus and cardiac postoperative complication. | aspergillus is a ubiquitous fungus which can cause a variety of clinical syndromes. this fungus has emerged as agent of systemic infections and has therefore gained considerable public health importance. this paper describes two cases of invasive aspergillosis caused by a. fumigatus in immuno-suppressed patients and underscores the importance of early identification of aspergillus infection associated with systemic lupus erythematosus and cardiac postoperative complications. | 2009 | 24031340 |
| isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of salix alba linn. in trans himalayan region of himachal pradesh. | phosphate solubilising microorganisms (psm) (bacteria and fungi) associated with salix alba linn. from lahaul and spiti valleys of himachal pradesh were isolated on pikovskaya (pvk), modified pikovskaya (mpvk) and national botanical research institute agar (nbrip) media by spread plating. the viable colony count of p-solubilising bacteria (psb) and fungi (psf) was higher in rhizosphere than that of non-rhizosphere. the frequency of psm was highest on mpvk followed by nbrip and pvk agar. the maxi ... | 2008 | 23100719 |
| ability of a commercial feed additive to modulate expression of innate immunity in sheep immunosuppressed with dexamethasone. | in the first study, we tested the ability of a commercial feed additive (omnigen-af) to affect markers of innate immunity in immunosuppressed sheep and the ability of a pathogen challenge (mould) to affect the immune response to the additive. treatments consisted of (1) control, (2) immunosuppressed with dexamethasone (dex), (3) immunosuppressed plus the feed additive, (4) immunosuppressed plus aspergillus fumigatus and (5) immunosuppressed, a. fumigatus and the additive. animal health was monit ... | 2007 | 22444796 |
| immune response to n-terminal and c-terminal deletion mutants of aspergillus fumigatus major allergen asp f 3. | the ubiquitous fungus aspergillus fumigatus causes allergic rhinitis, asthma, sinusitis and allergic bronchopulmonary aspergillosis. a number of major allergens from a. fumigatus are purified, but their structure-function role in the pathogenesis of disease is not known. such information is essential for devising alternative therapy of fungal allergic diseases. in the present study, n-terminal and c-terminal deletion mutants ofasp f 3 were constructed and their immunopathological responses studi ... | 2006 | 23105608 |
| immunoproteomic analysis of secretory proteins ofaspergillus fumigatus with specific ige immunoreactivity. | allergenic/antigenic proteins are known to induce type i and type iii hypersensitivity reactions leading to allergic bronchopulmonary aspergillosis (abpa) in immunocompetent host. the common structural features or intrinsic properties of the allergens/antigens leading to allergenicity in a host are not well understood. in the current report, comparative analysis of proteins on two dimensional gel electrophoresis (2d-3) and specific ige immunoblots ofa. fumigatus secretory proteins (1(st), 2(nd) ... | 2006 | 23105607 |
| postoperative bone graft displacement: an unusual sign of infection following posterior spinal fusion. | we describe a case of a spinal fusion site infection, which was first suggested on plain radiographs due to interval displacement and partial dissolution of the bone graft material. these radiographic findings occurred 4 weeks before the infection became clinically evident. cultures taken during eventual surgical debridement grew aspergillus fumigatus. this case emphasizes the importance of noting changes in bone graft material in addition to the routine evaluation of alignment and hardware in p ... | 2006 | 27298675 |
| antifungal activity of lactic acid bacteria isolated from kimchi against aspergillus fumigatus. | more than 120 isolates of lactic acid bacteria obtained from kimchi was screened for antifungal activity against aspergillus fumigatus. approximately 10% of the isolates showed inhibitory activity and only 4.16% (five isolates) exhibited strong activity against the indicator fungus a. fumigatus. the five isolates showed a wide rang of antifungal activity against a. flavus, fusarium moniliforme, penicillium commune, and rhizopus oryzae. they were identified by 16s rdna sequencing as lactobacillus ... | 2005 | 24049503 |
| identification and characterization of polyubiquitin gene from cdna library of aspergillus fumigatus. | aspergillus fumigatus (afu) causes allergic and invasive forms of diseases in humans. in order to identify genes relevant for pathogenesis, a total of 235 cdna clones were randomly selected and sequenced from cdna library of afu. one of the partially sequenced cdna clones was homologous to polyubiquitin. sequencing of the complete cdna clone showed an open reading frame of 912 bases. comparison with genomic sequence of afu using blastn program, revealed that polyubiquitin gene comprises of 992 b ... | 2005 | 23105524 |
| incidence of fungal infections in chronic maxillary sinusitis. | fifty patients with chronic maxillary sinusitis were included in our study. antrat aspirate of chronically inflamed maxillary sinuses collected by antrat puncture were subjected to fungal culture. the study revealed the presence of fungi in 3 cases. aspergillus fumigatus was isolated in 2 cases while candida albicans was found in 1 case. the role of fungi, particularly aspergillus as pathogen is discussed in the context of antibiotics and immuno-suppressive therapy and local predisposing factors ... | 2000 | 23119648 |
| mycotic spondylitis in broilers caused by aspergillus fumigatus resulting in partial anterior and posterior paralysis. | this report describes outbreaks of mycotic spondylitis in two broiler flocks of 17 and 19 days old. postmortem examination showed kyphosis and compression of the spinal cord, due to osteomyelitis of either the cervical or the first thoracic vertebrae. these lesions resulted in partial anterior paralysis affecting wing mobility and posterior paralysis. aspergillus fumigatus was cultured from affected vertebrae. | 1999 | 26911603 |
| biochemical characterization of an immunodominant allergen/antigen ofaspergillus fumigatus. | an 18kda protein was identified as a major immunodominant allergen/antigen secreted by a wild type isolate and various clinical isolates ofa. fumigatus. the protein was purified to homogeneity and the n-terminal amino acid was found to be alanine. the n-terminal 20 amino acid sequence of 18kda was found to be similar to restrictocin, a cytotoxin secreted byaspergillus restrictus. mass spectroscopic analysis of the purified allergen revealed a molecular size of 17.01 kda. immunoreactivity of the ... | 1999 | 23105213 |
| detection ofaspergillus fumigatus based on asp fl gene in clinical specimens. | oligonucleotide primers were synthesised based on the gene sequence of an 18 kda allergen/antigen ofa. fumigatus isolated from a pathogenic strain. polymerase chain reaction (pcr) was carried out using the forward and reverse primers and genomic dna ofa. fumigatus, a. flavus anda. niger as template. this resulted in a pcr product of 480 bp with onlya. fumigatus. the absence of pcr product ina. flavus anda. niger with the primers of asp fl facilitated use of these primers for detection ofa. fumig ... | 1999 | 23105210 |
| a circovirus-like infection in geese related to a runting syndrome. | diagnostic investigations in a large commercial flock of geese with a history of increased losses and runting, lead to the negative contrast electron microscopic detection of circovirus-like viruses in the lymphoreticular tissue in eight of nine selected geese with growth retardation. with the exception of a mild cloudiness of some air sacs, macroscopic changes pointing to an infection were missed. histopathological changes concentrated on the lymphoreticular tissue. lymphocytic depletion and hi ... | 1999 | 26911506 |