| complete bordetella avium, bordetella hinzii and bordetella trematum lipid a structures and genomic sequence analyses of the loci involved in their modifications. | endotoxin is recognized as one of the virulence factors of the bordetella avium bird pathogen, and characterization of its structure and corresponding genomic features are important for an understanding of its role in pathogenicity and for an improved general knowledge of bordetella spp virulence factors. the structure of the biologically active part of b. avium lps, lipid a, is described and compared to those of another bird pathogen, opportunistic in humans, bordetella hinzii, and to that of b ... | 2014 | 24127384 |
| the extent of the temperature-induced membrane remodeling in two closely related bordetella species reflects their adaptation to diverse environmental niches. | changes in environmental temperature represent one of the major stresses faced by microorganisms as it affects the function of the cytoplasmic membrane. in this study we have analyzed the thermal adaptation in two closely related respiratory pathogens bordetella pertussis and bordetella bronchiseptica. while b. pertussis represents a pathogen strictly adapted to the human body temperature, b. bronchiseptica causes infection in a broad range of animals and survives also outside of the host. we ap ... | 2017 | 28348085 |
| bordetella plrsr regulatory system controls bvgas activity and virulence in the lower respiratory tract. | bacterial pathogens coordinate virulence using two-component regulatory systems (tcs). the bordetella virulence gene (bvgas) phosphorelay-type tcs controls expression of all known protein virulence factor-encoding genes and is considered the "master virulence regulator" in bordetella pertussis, the causal agent of pertussis, and related organisms, including the broad host range pathogen bordetella bronchiseptica we recently discovered an additional sensor kinase, plrs [for persistence in the low ... | 2017 | 28167784 |
| essential role of bordetella nadc in a quinolinate salvage pathway for nad biosynthesis. | nicotinamide adenine dinucleotide (nad) is produced via de novo biosynthesis pathways and by salvage or recycling routes. the classical bordetella bacterial species are known to be auxotrophic for nicotinamide or nicotinic acid. this study confirmed that bordetella bronchiseptica, bordetella pertussis and bordetella parapertussis have the recycling/salvage pathway genes pnca and pncb, for use of nicotinamide or nicotinic acid, respectively, for nad synthesis. although these bordetellae lack the ... | 2017 | 27783449 |
| functional identification of putrescine c- and n-hydroxylases. | the small polyamine putrescine (1,4-diaminobutane) is ubiquitously and abundantly found in all three domains of life. it is a precursor, through n-aminopropylation or n-aminobutylation, for biosynthesis of the longer polyamines spermidine, sym-homospermidine, spermine, and thermospermine and longer and branched chain polyamines. putrescine is also biochemically modified for purposes of metabolic regulation and catabolism, e.g. n-acetylation and n-glutamylation, and for incorporation into special ... | 2016 | 27541336 |
| diversity of secretion systems associated with virulence characteristics of the classical bordetellae. | secretion systems are key virulence factors, modulating interactions between pathogens and the host's immune response. six potential secretion systems (types 1-6; t1ss-t6ss) have been discussed in classical bordetellae, respiratory commensals/pathogens of mammals. the prototypical bordetella bronchiseptica strain rb50 genome seems to contain all six systems, whilst two human-restricted subspecies, bordetella parapertussis and bordetella pertussis, have lost different subsets of these. this impli ... | 2015 | 26459829 |
| structure activity characterization of bordetella petrii lipid a, from environment to human isolates. | bordetella petrii, a facultative anaerobic species, is the only known member of the bordetella genus with environmental origin. however it was also recently isolated from humans. the structures of the b. petrii lipid a moieties of the endotoxins were characterized here for the first time for an environmental strain and compared to that of human isolates. characterization was achieved using chemical analyses, gas chromatography-mass spectrometry, and matrix assisted laser desorption ionisation ma ... | 2016 | 26164553 |
| prevalence of bordetella holmesii and bordetella bronchiseptica in respiratory tract samples from belgian patients with pertussis-like symptoms by sensitive culture method and mass spectrometry. | insertion sequences is481 and is1001 are targets for molecular detection of respectively bordetella pertussis and bordetella parapertussis. there is a raising concern about specificity of these targets due to sequence similarity with bordetella holmesii and bordetella bronchiseptica. the likelihood of false (para)pertussis diagnoses should be correlated with the prevalence of these organisms in the respiratory tract (rt). from october 2010 until september 2011, 2,207 rt samples were submitted to ... | 2016 | 24579240 |
| epidemiology of whooping cough & typing of bordetella pertussis. | bordetella pertussis is a gram-negative human-restricted bacterium that evolved from the broad-range mammalian pathogen, bordetella bronchiseptica. it causes whooping cough or pertussis in humans, which is the most prevalent vaccine-preventable disease worldwide. the introduction of the pertussis whole-cell vaccination for young children, followed by the introduction of the pertussis acellular vaccination (along with booster vaccination) for older age groups, has affected the bacterial populatio ... | 2013 | 24199799 |
| in-vitro and in-vivo analysis of the production of the bordetella type three secretion system effector a in bordetella pertussis, bordetella parapertussis and bordetella bronchiseptica. | bordetella pertussis, bordetella parapertussis, and bordetella bronchiseptica are three closely related pathogens. they all possess the gene coding for the bordetella type three secretion system effector a (btea) toxin that became a focus of interest since it was demonstrated that b. pertussis japanese non-vaccine-type isolates produce btea unlike vaccine-type isolates. we thus explored the in-vitro production of btea in b. pertussis isolates collected in france during periods of different vacci ... | 2013 | 23470234 |
| dual mechanism of protection by live attenuated bordetella pertussis bpze1 against bordetella bronchiseptica in mice. | bordetella bronchiseptica, a gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including man, and no human vaccine is currently available. acellular pertussis vaccines protect poorly against b. bronchiseptica, although they contain cross-reactive antigens. we have recently developed bordetella pertussis bpze1, a novel, live attenuated pertussis vaccine, currently completing phase i clinical trials in humans, and found that it protects agai ... | 2012 | 22814407 |
| development of vaccines against pertussis caused by bordetella holmesii using a mouse intranasal challenge model. | bordetella holmesii is recognized as the third causative agent of pertussis (whooping cough) in addition to bordetella pertussis and bordetella parapertussis. pertussis caused by b. holmesii is not rare around the world. however, to date, there is no effective vaccine against b. holmesii. we examined the protective potency of pertussis vaccines available in japan and vaccines prepared from b. holmesii. a murine model of respiratory infection was exploited to evaluate protective potency. no japan ... | 2016 | 27515393 |
| bordetella holmesii: still emerging and elusive 20 years on. | since the first description of bordetella holmesii in 1995, almost 100 publications have contributed to the increasing knowledge of this emerging bacterium. although first reported to induce bacteremia mainly in immunocompromised patients, it has also been isolated in healthy persons and has shown the capacity to induce pertussis-like symptoms and other clinical entities, such as meningitis, arthritis, or endocarditis. respiratory diseases are generally less severe than those induced by bordetel ... | 2016 | 27227292 |
| bordetella holmesii infection: current knowledge and a vision for future research. | bordetella holmesii is a recently recognized gram-negative bacterium causing both pertussis-like respiratory symptoms and invasive infections, such as bacteremia, pneumonia, meningitis, arthritis, pericarditis and endocarditis. few data are available on its epidemiological characteristics, mostly related to respiratory infections. however, these are frequently misdiagnosed as a bordetella pertussis infection as most diagnostic tests routinely used are not species-specific, thus biasing the epide ... | 2015 | 26065696 |
| pertussis: relevant species and diagnostic update. | pertussis is increasing due to multiple factors including increasing awareness by clinicians, decreased effectiveness of vaccines, and improved testing. while bordetella pertussis is the causative agent of pertussis other bordetella species such as b. parapertussis, and b. holmesii, have been associated with pertussis-like illness. laboratory diagnosis is made using various tests with molecular methods supplanting culture due to increased sensitivity. serology is useful but standardized methods ... | 2014 | 24856526 |
| [simultaneous screening method for bordetella species by conventional pcr assay]. | a simultaneous screening method using conventional pcr was developed for the detection and discrimination of bordetella pertussis, bordetella parapertussis, and bordetella holmesii. a formulated multiprex method employing 4 kinds of paired primers on amplification of 4 corresponding different insertion sequences (is481, is1001, is1002 and his1001) enabled rapid screening and identification. the detection limits of each dna extracted from 3 kinds of bordetella species were 5fg/microl for each. ob ... | 2013 | 24483018 |
| occurrence of 3 bordetella species during an outbreak of cough illness in ohio: epidemiology, clinical features, laboratory findings and antimicrobial susceptibility. | an increase in laboratory diagnosis of pertussis was noted in central ohio during 2010. diagnosis was made using a polymerase chain reaction assay targeting the multicopy insertion sequence is481, which is found in both bordetella pertussis (bp) and bordetella holmesii (bh). an increase in specimens testing positive for bordetella parapertussis (bpp) using insertion sequence is1001 was also noted. | 2014 | 24445823 |
| whooping cough in south-east romania: a 1-year study. | the incidence of whooping cough in romania is substantially underestimated, and, as noted by the health authorities, this is mostly due to the lack of both awareness and biological diagnosis. we conducted a 1-year study in bucharest in order to assess the circulation of bordetella pertussis, the main etiological agent of whooping cough. fifty-one subjects suspected of whooping cough were enrolled. culture, real-time pcr, and enzyme-linked immunosorbent assay were used for laboratory diagnosis. w ... | 2014 | 24355701 |
| evaluation of pcr methods for the diagnosis of pertussis by the european surveillance network for vaccine-preventable diseases (euvac.net). | this study aimed to evaluate the performance of polymerase chain reaction (pcr) methods used for the diagnosis of pertussis in laboratories within europe in 2011. national reference laboratories in 25 european countries were contacted and a total of 24 laboratories from 19 countries agreed to participate in the study. a panel of seven samples of dna from bordetella pertussis, bordetella parapertussis and bordetella holmesii plus a negative control were distributed and analysed according to the r ... | 2013 | 23609510 |
| epidemiologic and laboratory features of a large outbreak of pertussis-like illnesses associated with cocirculating bordetella holmesii and bordetella pertussis--ohio, 2010-2011. | during 9 may 2010-7 may 2011, an outbreak of pertussis-like illness (incidence, 80 cases per 100 000 persons) occurred in franklin county, ohio. the majority of cases were identified by is481-directed polymerase chain reaction (pcr), which does not differentiate among bordetella species. we sought to determine outbreak etiology and epidemiologic characteristics. | 2013 | 23087388 |
| bronchitis caused by bordetella holmesii in a child with asthma misdiagnosed as mycoplasmal infection. | we report a case of a bronchitis caused by bordetella holmesii in a 2-year-old girl with asthma. the patient had a moderate fever and productive cough, and her condition was initially diagnosed as mycoplasmal bronchitis on the basis of her clinical symptoms and rapid serodiagnosis of mycoplasmal infection. she was treated with a bronchodilator and clarithromycin, which resulted in complete recovery. however, after the initial diagnosis, nucleic acid amplification tests of her sputum showed the a ... | 2013 | 23053501 |
| periodic solutions in an sirws model with immune boosting and cross-immunity. | incidence of whooping cough, an infection caused by bordetella pertussis and bordetella parapertussis, has been on the rise since the 1980s in many countries. immunological interactions, such as immune boosting and cross-immunity between pathogens, have been hypothesised to be important drivers of epidemiological dynamics. we present a two-pathogen model of transmission which examines how immune boosting and cross-immunity can influence the timing and severity of epidemics. we use a combination ... | 2016 | 27575466 |
| two distinct episodes of whooping cough caused by consecutive bordetella pertussis and bordetella parapertussis infections in a fully immunized healthy boy. | we describe a 5-year-old, fully immunized boy with polymerase chain reaction-proven consecutive bordetella pertussis and bordetella parapertussis infections causing typical whooping cough at the age of 2 and 5 years, respectively. neither pertussis immunization nor disease provides reliable immunity against further episodes of whooping cough. | 2016 | 27391699 |
| bordetella pertussis. | pertussis is a highly infectious vaccine-preventable cough illness that continues to be a significant source of morbidity and mortality around the world. the majority of human illness is caused by bordetella pertussis, and some is caused by bordetella parapertussis. bordetella is a gram-negative, pleomorphic, aerobic coccobacillus. in the past several years, even countries with high immunization rates in early childhood have experienced rises in pertussis cases. reasons for the resurgence of rep ... | 2016 | 27337481 |
| incidence and diagnosis of pertussis in south african children hospitalized with lower respiratory tract infection. | the incidence of pertussis in children in low- and middle-income countries is poorly described. this study aimed to prospectively investigate the incidence of pertussis in south african children hospitalized with lower respiratory tract infection (lrti). | 2016 | 26967813 |
| bordetella pertussis, b. parapertussis, vaccines and cycles of whooping cough. | whooping cough is a vaccine-preventable disease due to bordetella pertussis and b. parapertussis. this highly contagious respiratory disease occurs through epidemic cycles every 3-5 years and vaccination did not change this frequency. models suggest that the cyclic increase of susceptibles is linked to demographic differences and different vaccine coverage. however, differences in surveillance of the disease as well as adaptation of the agents of the disease to their human hosts and to vaccine p ... | 2015 | 26242280 |
| widespread bordetella parapertussis infections-wisconsin, 2011-2012: clinical and epidemiologic features and antibiotic use for treatment and prevention. | during october 2011-december 2012, concurrent with a statewide pertussis outbreak, 443 bordetella parapertussis infections were reported among wisconsin residents. we examined clinical features of patients with parapertussis and the effect of antibiotic use for treatment and prevention. | 2015 | 26113655 |
| bordetella parapertussis outbreak in bisham, pakistan in 2009-2010: fallout of the 9/11 syndrome. | pertussis or whooping cough is a highly contagious community disease mainly caused by bordetella pertussis and b. parapertussis. we report a minor outbreak of whooping cough (2009-2010) in symptomatic subjects from bisham, near swat, khyber pukhtoonkhawa province, pakistan. interestingly, our results show that all the culture-positive isolates (n = 21) collected from children (average age 3·46 years), were identified as b. parapertussis after routine identification tests and pcr is481, is1001 an ... | 2015 | 25583126 |
| evaluation of 3 analyte-specific reagents for detection of bordetella pertussis and bordetella parapertussis in clinical specimens. | the performance of 3 analyte-specific reagents (asrs), elitech biosciences, eragen biosciences, and focus diagnostic, was evaluated for detection of bordetella pertussis (bp) and bordetella parapertussis (bpp) in nasopharyngeal swab specimens. a total of 104 frozen, leftover clinical specimens obtained from pediatric patients during 2011-2012 were included in this study. performance was compared to the bordetella real-time polymerase chain reaction (pcr) laboratory-developed test (ldt). the posi ... | 2014 | 25239539 |
| pertussis diagnostics: overview and impact of immunization. | in all vaccinated populations, infections with bordetella pertussis and bordetella parapertussis continue to cause infections in unvaccinated infants and children, as well as in adolescents and adults with waning immunity. thus in patients with longer lasting coughs a diagnosis of pertussis should be entertained irrespective of their vaccination status. due to the non-specific clinical symptoms, clinically suspected cases of pertussis must be verified by laboratory methods. hyperleukocytosis may ... | 2014 | 25142439 |
| pertussis vaccination and whooping cough: and now what? | pertussis or whooping cough is a respiratory disease caused by bordetella pertussis or bordetella parapertussis that are only known to infect humans. this severe and acute respiratory disease presents epidemic cycles and became a vaccine-preventable disease in the 1940s/1950s when developed countries introduced vaccination. the first type of vaccine developed against this disease was a whole-cell pertussis (wp) vaccine containing inactivated b. pertussis bacteria. most developed countries produc ... | 2014 | 25020131 |
| bordetella pertussis and pertactin-deficient clinical isolates: lessons for pertussis vaccines. | bordetella pertussis causes whooping cough in humans, a highly transmissible respiratory disease life threatening for unvaccinated infants. vaccination strategies were thus introduced worldwide with great success in developed countries reaching high vaccine coverage with efficacious vaccines. in the late 20th/early 21st century, acellular pertussis vaccines replaced whole cell pertussis vaccines but b. pertussis still circulates and evolves in humans, its only known reservoir. the latest transfo ... | 2014 | 24953157 |
| prevalence of asymptomatic bordetella pertussis and bordetella parapertussis infections among school children in china as determined by pooled real-time pcr: a cross-sectional study. | studies have documented that older children and adolescents act as a reservoir of bordetella pertussis infection for young infants who have not yet completed their primary immunization schedule. asymptomatic pertussis infection has been reported during outbreaks. this cross-sectional study aimed to investigate whether b. pertussis and bordetella parapertussis can colonize the nasopharynx of healthy school children, using culture and pooled real-time pcr with targets for insertion sequences is481 ... | 2014 | 24520981 |
| development of a multiplex real-time pcr assay for the detection of bordetella pertussis and bordetella parapertussis in a single tube reaction. | pertussis is an infectious respiratory disease caused by the fastidious bacterium bordetella pertussis, which may infect unvaccinated, previously vaccinated children, and adults in whom immunity has waned. infants are at a particular risk for severe disease and complications. bordetella parapertussis may cause a similar illness, however the symptoms are less severe and of shorter duration. pertussis is a highly contagious disease and early diagnosis is essential. studies have shown that pcr is 2 ... | 2014 | 24333686 |
| the 2009 pandemic influenza a(h1n1) coincides with changes in the epidemiology of other viral pathogens causing acute respiratory tract infections in children. | in germany, the outbreak of the novel pandemic 2009 influenza a(h1n1) virus a(h1n1)pdm09 caused a wave of high activity between november 2009 and january 2011. the aim of this study was to investigate the prevalence of 19 respiratory pathogens in children hospitalized for lower respiratory tract infections during the winter influenza seasons of 2009/2010 and 2010/2011 and to observe a possible impact of influenza a(h1n1)pdm09 on the epidemiology of other epidemic viruses. | 2014 | 24150959 |
| [diagnosis of whooping cough by serology and real-time pcr]. | the goal of this study is to summarize the results of the detection of bordetella pertussis (bp) and bordetella parapertussis (bpp) by a real-time polymerase chain reaction (rt-pcr) assay and serological methods. in 2008-2010, 73 patients of the department of clinical immunology and allergology of the centre for immunology and microbiology, public health institute in ústí nad labem were screened for pertussis. they were selected according to the who and ecdc criteria, i. e. they presented with a ... | 2013 | 24116698 |
| outer membrane vesicles derived from bordetella parapertussis as an acellular vaccine against bordetella parapertussis and bordetella pertussis infection. | bordetella parapertussis, a close related species of b. pertussis, can also cause the disease named pertussis or whooping cough. the number of cases caused by this related pathogen has risen sustained in the last years. the widely used cellular (wp) or acellular (ap) pertussis vaccines have little or no efficacy against b. parapertussis. in an effort to devise an effective acellular vaccine against b. parapertussis infection, outer membrane vesicles (omvs) were obtained from b. parapertussis. pr ... | 2013 | 24012570 |
| re-emergence of pertussis: what are the solutions? | whooping cough, due to bordetella pertussis and bordetella parapertussis, is an important cause of childhood morbidity and mortality. despite widespread pertussis immunization in childhood, there are an estimated 50 million cases and 300,000 deaths due to pertussis globally each year. infants who are too young to be vaccinated, children who are partially vaccinated and fully-vaccinated persons with waning immunity are especially vulnerable to disease. since pertussis is one of the vaccine-preven ... | 2012 | 23249233 |
| evolution of french bordetella pertussis and bordetella parapertussis isolates: increase of bordetellae not expressing pertactin. | bordetella pertussis and bordetella parapertussis are closely related bacterial agents of whooping cough. whole-cell pertussis (wp) vaccine was introduced in france in 1959. acellular pertussis (ap) vaccine was introduced in 1998 as an adolescent booster and was rapidly generalized to the whole population, changing herd immunity by specifically targeting the virulence of the bacteria. we performed a temporal analysis of all french b. pertussis and b. parapertussis isolates collected since 2000 u ... | 2012 | 22717007 |
| prevalence of bordetella pertussis and bordetella parapertussis infections in tunisian hospitalized infants: results of a 4-year prospective study. | the prevalence of pertussis in tunisia remains undetermined essentially because of the unavailability of a basic laboratory diagnostic service. specific diagnostic tools were applied for the first time in a tunisian prospective study in order to get a first estimation of the prevalence of bordetella pertussis/parapertussis infections and to evaluate their use to determine the epidemiologic characteristics of these infections in tunisian infants. between 2007 and 2011, a total of 626 samples from ... | 2012 | 22313629 |
| [seroprevalence of antibodies against lipopolysaccharides of bordetella parapertussis in patients with prolonged caught in poland]. | bordetella parapertussis is a bacterium closely related to bordetella pertussis, also causes a pertussis - like symptoms in humans. because of unsatisfactory level of routine microbiological diagnosis of b. parapertussis infections in poland most of parapertussis cases are not reported. the aim of the presented study was to investigate incidence of b. parapertussis in patients with cough in poland using serology method. | 2012 | 23484419 |
| assessment of safety and efficacy against bordetella pertussis of a new tetanus-reduced dose diphtheria-acellular pertussis vaccine in a murine model. | tetanus-reduced dose diphtheria-acellular pertussis (tdap) vaccination during adolescence was introduced in response to the resurgence of pertussis in various countries. a new tdap vaccine was manufactured in korea as a countermeasure against a predicted tdap vaccine shortage. this study was performed to evaluate the immunogenicity, safety, and protection efficacy against bordetella pertussis of the new tdap vaccine in a murine model. | 2017 | 28376777 |
| seroprevalence of bordetella pertussis antibodies in adults in hungary: results of an epidemiological cross-sectional study. | pertussis (whooping cough) is well known to be underreported, particularly among adults, who can act as an infectious reservoir, potentially putting susceptible newborns at risk of serious illness. the purpose of this study was to estimate the seroprevalence of pertussis in adults in hungary. | 2017 | 28376739 |
| prevalence of atypical pathogens in patients with cough and community-acquired pneumonia: a meta-analysis. | community-acquired pneumonia (cap), acute cough, bronchitis, and lower respiratory tract infections (lrti) are often caused by infections with viruses or streptococcus pneumoniae. the prevalence of atypical pathogens mycoplasma pneumoniae, chlamydophila pneumoniae, legionella pneumophila, and bordetella pertussis among patients with these illnesses in the ambulatory setting has not been previously summarized. we set out to derive prevalence information from the existing literature. | 2016 | 28376442 |
| dot immunoassay for the simultaneous determination of postvaccination immunity against pertussis, diphtheria, and tetanus. | a dot immunoassay for simultaneous semiquantitative detection of igg against tetanus toxoid (ttx) and diphtheria toxoid (dtx) and qualitative detection of anti-bordetella pertussis iggs in human blood serum using carbon nanoparticles functionalized with streptococcal protein g was developed. inactivated b. pertussis cells in suspension form were used as an antigen in the immunoassay. pertussis, tetanus, and diphtheria antigens were separately spotted onto nitrocellulose strips, and then the immu ... | 2017 | 28374129 |
| analysis of membrane protein interactions with a bacterial adenylate cyclase-based two-hybrid (bacth) technique. | the bacterial two-hybrid (bacth, for "bacterial adenylate cyclase-based two-hybrid") technique is a simple and fast genetic approach to analyze protein-protein interactions in vivo. in this system, the proteins of interest are genetically fused to two complementary fragments from the catalytic domain of bordetella pertussis adenylate cyclase and co-expressed in strains of escherichia coli deficient in adenylate cyclase. association of the hybrid proteins restores synthesis of cyclic amp (camp), ... | 2017 | 28369675 |
| bordetella pertussis infections in travelers: data from the geosentinel global network. | pertussis is a highly contagious, vaccine-preventable respiratory infection that is endemic worldwide. there are limited data regarding the occurrence of pertussis in travelers. the objective of this study is to identify travel-related pertussis cases reported to the geosentinel surveillance network. | 2017 | 28355615 |
| live pertussis vaccines: will they protect against carriage and spread of pertussis? | pertussis is a severe respiratory disease that can be fatal in young infants. its main aetiological agent is the gram-negative micro-organism bordetella pertussis. vaccines against the disease have been in use since the 1950s, and global vaccination coverage has now reached more than 85%. nevertheless, the disease has not been controlled in any country, and has even made a spectacular come-back in the industrialized world, where the first-generation whole-cell vaccines have been replaced by the ... | 2016 | 28341014 |
| significant decrease in pertactin-deficient bordetella pertussis isolates, japan. | prevalence of pertactin-lacking bordetella pertussis isolates has been observed worldwide. in japan, however, we found that the frequency of pertactin-deficient isolates in 2014-2016 (8%) was significantly lower than the frequency in 2005-2007 (41%), 2008-2010 (35%), and 2011-2013 (25%). this reduction was closely associated with changes in genotypes. | 2017 | 28322702 |
| gtl001, a bivalent therapeutic vaccine against human papillomavirus 16 and 18, induces antigen-specific cd8+ t cell responses leading to tumor regression. | prophylactic vaccines are available for women and girls not yet infected with hpv, but women already infected with hpv need a treatment to prevent progression to high-grade cervical lesions and cancer. gtl001 is a bivalent therapeutic vaccine for eradicating hpv-infected cells that contains hpv16 e7 and hpv18 e7 both fused to detoxified adenylate cyclase from bordetella pertussis, which binds specifically to cd11b+ antigen-presenting cells. this study examined the ability of therapeutic vaccinat ... | 2017 | 28301611 |
| what is wrong with pertussis vaccine immunity? the problem of waning effectiveness of pertussis vaccines. | pertussis is resurgent in some countries, particularly those in which children receive acellular pertussis (ap) vaccines in early infancy and boosters later in life. immunologic studies show that, whereas whole-cell pertussis (wp) vaccines orient the immune system toward th1/th17 responses, acellular pertussis vaccines orient toward th1/th2 responses. although ap vaccines do provide protection during the first years of life, the change in t-cell priming results in waning effectiveness of ap as e ... | 2017 | 28289064 |
| what is wrong with pertussis vaccine immunity? inducing and recalling vaccine-specific immunity. | the high incidence of pertussis in vaccinated adolescents suggests the failing of immune memory. we argue that acellular pertussis vaccines generate memory cells that are effectively reactivated by boosters better than by bordetella pertussis exposure. we propose that there are two main causes. one is the induction of vaccine-specific immunity rather than pathogen-specific immunity. the second is that strictly mucosal infections such as b. pertussis poorly reactivate memory b and t cells residin ... | 2017 | 28289058 |
| [investigation of seropositivity of bordetella pertussis in adults in a university hospital]. | whooping cough is a vaccine-preventable infectious diseases caused by bordetella pertussis/parapertussis. despite of routine immunization programs in the world, pertussis still remains endemic. recently unvaccinated or partially immunized infants have infected with this pathogen and also increase of incidence was observed in adolescents and adults. the source of pertussis in newborns are attributed to household, especially due to the family members. theaim of this study was to determine b.pertus ... | 2017 | 28283011 |
| recurrent apnea in an infant with pertussis due to household transmission. | bordetella pertussis causes life-threatening apnea in infants. lymphocytosis is an important clue for diagnosis and for determining the severity of pertussis. antibiotics do not shorten or ameliorate the disease and only decrease the risk of transmission. antepartum maternal immunization is important for preventing pertussis in infants. | 2017 | 28265381 |
| letter to the editor: verification of the rida(®)gene bordetella real-time pcr kit for the detection of bordetella pertussis and other bordetella species from nasopharyngeal swabs collected in regan-lowe transport medium. | | 2017 | 28249928 |
| safety evaluation of a vaccine: effect in maternal reproductive outcome and fetal anomaly frequency in rats using a leishmanial vaccine as a model. | while the immunogenic potential of the vaccination against infectious diseases was extensively shown, data on the safety assessment of recombinant proteins in vaccine formulations administered during pregnancy are still scarce. in the current study, the antigenicity of a vaccine against leishmaniasis (based on leishmania braziliensis recombinant protein peroxidoxin) during pregnancy and possible maternal reproductive outcomes and fetal anomalies after immunization with a leishmanial vaccine or a ... | 2017 | 28249007 |
| meta-analysis of pulmonary transcriptomes from differently primed mice identifies molecular signatures to differentiate immune responses following bordetella pertussis challenge. | respiratory infection with bordetella pertussis leads to severe effects in the lungs. the resulting immunity and also immunization with pertussis vaccines protect against disease, but the induced type of immunity and longevity of the response are distinct. in this study the effects of priming, by either vaccination or infection, on a subsequent pathogen encounter were studied. to that end, three postchallenge transcriptome datasets of previously primed mice were combined and compared to the resp ... | 2017 | 28243609 |
| characterisation of the bordetella pertussis secretome under different media. | our understanding of the bordetella pertussis secretome remains limited including the role of different growth conditions in the secretome. in this study the secretome of l1423, a clinical isolate from the 2008-2012 australian epidemic, cultured on stainer-scholte (ss) and thalen-ijssel (thijs) media for 12h was characterised using liquid chromatography-mass spectrometry (lc-ms/ms). in the supernatant, lc-ms/ms identified 260 proteins with 143 bioinformatically predicted to be secreted. eighty p ... | 2017 | 28242451 |
| zn(2+)-dependent autocatalytic activity of the bordetella pertussis cyaa-hemolysin. | proteolytic degradation of the ∼100-kda isolated rtx (repeat-in-toxin) subdomain (cyaa-rtx) of the bordetella pertussis cyaa-hemolysin (cyaa-hly) was evidently detected upon solely-prolonged incubation. here, a truncated cyaa-hly fragment (cyaa-hp/bi) containing hydrophobic and acylation regions connected with the first rtx block (bi1015-1088) was constructed as a putative precursor for investigating its potential autocatalysis. the 70-kda his-tagged cyaa-hp/bi fragment which was over-expressed ... | 2017 | 28238785 |
| short-read whole genome sequencing for laboratory-based surveillance of bordetella pertussis. | bordetella pertussis (bp) is a gram-negative bacterium that causes respiratory infections in humans. ongoing molecular surveillance of bp acellular vaccine (ap) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. methods currently used to characterize ap components are relatively labour intensive and low throughput. to address this challenge, we sought to derive ap antigen genotypes from minimally processed short ... | 2017 | 28228490 |
| the characterization of bordetella pertussis strains isolated in the central-western region of brazil suggests the selection of a specific genetic profile during 2012-2014 outbreaks. | pertussis is a worldwide acute respiratory disease caused by the bacterium bordetella pertussis. despite high vaccine coverage, the bacterium continues to circulate in populations and is still one of the most common vaccine-preventable diseases. in brazil, pertussis incidence has presented a significant decrease since 1990 but since 2011 a sudden increase in incidence has been observed. thus, the aim of this study was to perform a molecular epidemiological characterization of b. pertussis strain ... | 2017 | 28219454 |
| gtl001 and bivalent cyaa-based therapeutic vaccine strategies against human papillomavirus and other tumor-associated antigens induce effector and memory t-cell responses that inhibit tumor growth. | gtl001 is a bivalent therapeutic vaccine containing human papillomavirus (hpv) 16 and hpv18 e7 proteins inserted in the bordetella pertussis adenylate cyclase (cyaa) vector intended to prevent cervical cancer in hpv-infected women with normal cervical cytology or mild abnormalities. to be effective, therapeutic cervical cancer vaccines should induce both a t cell-mediated effector response against hpv-infected cells and a robust cd8(+) t-cell memory response to prevent potential later infection. ... | 2017 | 28196735 |
| stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, cyaa, from bordetella pertussis. | bordetella pertussis, the causative agent of whooping cough, secretes an adenylate cyclase toxin, cyaa, which invades eukaryotic cells and alters their physiology by camp overproduction. calcium is an essential cofactor of cyaa, as it is the case for most members of the repeat-in-toxins (rtx) family. we show that the calcium-bound, monomeric form of cyaa, hcyaam, conserves its permeabilization and haemolytic activities, even in a fully calcium-free environment. in contrast, hcyaam requires sub-m ... | 2017 | 28186111 |
| fine epitope mapping of two antibodies neutralizing the bordetella adenylate cyclase toxin. | adenylate cyclase toxin (act) is an important bordetella pertussis virulence factor that is not included in current acellular pertussis vaccines. we previously demonstrated that immunization with the repeat-in-toxin (rtx) domain of act elicits neutralizing antibodies in mice and discovered the first two antibodies to neutralize act activities by occluding the receptor-binding site. here, we fully characterize these antibodies and their epitopes. both antibodies bind act with low nanomolar affini ... | 2017 | 28177609 |
| protective role of passively transferred maternal cytokines against bordetella pertussis infection in newborn piglets. | maternal vaccination represents a potential strategy to protect both the mother and the offspring against life-threatening infections. this protective role has mainly been associated with antibodies, but the role of cell-mediated immunity, in particular passively transferred cytokines, is not well understood. here, using a pertussis model, we have demonstrated that immunization of pregnant sows with heat-inactivated bacteria leads to induction of a wide range of cytokines (e.g., tumor necrosis f ... | 2017 | 28167667 |
| the history of bordetella pertussis genome evolution includes structural rearrangement. | despite high pertussis vaccine coverage, reported cases of whooping cough (pertussis) have increased over the last decade in the united states and other developed countries. although bordetella pertussis is well known for its limited gene sequence variation, recent advances in long-read sequencing technology have begun to reveal genomic structural heterogeneity among otherwise indistinguishable isolates, even within geographically or temporally defined epidemics. we have compared rearrangements ... | 2017 | 28167525 |
| immune response and protective profile elicited by a multi-epitope chimeric protein derived from leptospira interrogans. | pathogenic leptospira is the causative agent of leptospirosis, a widely disseminated disease of human and veterinary concern. the development of vaccines that elicit cross-protective immunity through multiple leptospiral serovars has long been pursued. the aim of this study was to develop a novel chimeric multi-epitope fusion antigen, containing sequences of previously studied outer membrane proteins (omps) of leptospira. | 2017 | 28161462 |
| the proline residue at position 319 of bvgs is essential for bvgas activation in bordetella pertussis. | | 2017 | 28158456 |
| [a case of bronchiolitis obliterans secondary to human metapneumovirus bronchiolitis]. | human metapneumovirus (hmpv), formerly classified in paramyxoviridae family is now moved into pneumoviridae, which was described as a novel family. it causes upper and lower respiratory tract infections (lrtis) usually in children younger than five years old. the recent epidemiological studies indicated that hmpv is the second most frequently detected virus in lrtis of young children, following the respiratory syncytial virus (rsv). bronchiolitis obliterans (bo) is a chronic obstructive lung dis ... | 2016 | 28124966 |
| adverse events following primary and secondary immunisation with whole-cell pertussis: a systematic review protocol. | pertussis is a contagious respiratory illness caused by the bacterium bordetella pertussis. two types of vaccines are currently available against the disease: whole-cell pertussis (wp) and acellular pertussis (ap). with the shift of high-income countries from wp to ap as a result of adverse events following immunisation (aefi), an upsurge in reported cases of pertussis has been noticed. owing to this, it is proposed to use wp as a prime and ap for boost vaccination strategy. however, a compariso ... | 2017 | 28122832 |
| bordetella pertussis infection in children with severe pneumonia, philippines, 2012-2015. | a case-comparison study was conducted based on an observational study of severe pneumonia among hospitalized children in the philippines. the children, from 8days to 13years old and hospitalized with clinical diagnosis of severe or very severe pneumonia from august 2012 to february 2015, were recruited. nasopharyngeal swabs were collected from 1152 cases and b. pertussis were detected from 34 cases by pcr. pertussis-positive cases were more likely to have no fever, more than one week of coughing ... | 2017 | 28109708 |
| a new vaccine delivery vehicle and adjuvant candidate: bordetella pertussis inactivated whole cells entrapped in alginate microspheres. | there is no doubt about whole cell pertussis vaccine efficacy, but it is necessary to improve the vaccine quality specially to decreasing its toxicity by obtaining good immunogenicity with low bacteria number content. in this work, under optimum condition inactivated b. pertussis bacteria cells entrapped alginate microparticles were fabricated and in vivo immunogenicity and potency of new microparticle based vaccine evaluated in mice. microspheres loaded inactive b. pertussis bacterium cells wer ... | 2017 | 28079006 |
| population-based pertussis incidence and risk factors in infants less than 6 months in nepal. | pertussis is estimated to cause 2 percent of childhood deaths globally and is a growing public health problem in developed countries despite high vaccination coverage. infants are at greatest risk of morbidity and mortality. maternal vaccination during pregnancy may be effective to prevent pertussis in young infants, but population-based estimates of disease burden in infants are lacking, particularly in low-income countries. the objective of this study was to estimate the incidence of pertussis ... | 2017 | 28073985 |
| risk factors for pertussis in adults and teenagers in england. | pertussis is a vaccine-preventable respiratory infection caused by bordetella pertussis which can be fatal in infants. although high vaccine coverage led to prolonged disease control in england, a national outbreak of pertussis in 2011 led to the largest increase in over two decades, including a marked increase in cases aged ⩾15 years. a case-control study in four regions of england was undertaken to investigate risk factors for pertussis in adolescents and adults, specifically employment type a ... | 2017 | 28065204 |
| characteristics of bordetella pertussis infection among infantsand children admitted to paediatric intensive care units in greece: a multicentre, 11-year study. | to describe children with pertussis who required intensive care. | 2017 | 28058755 |
| whooping cough in adults: a series of severe cases. | bordetella pertussis is a gram-negative aerobic coccobacillus causing contagious respiratory tract disease called whooping cough. the virulence factors consist of pertussis toxin, filamentous hemagglutinin, fimbriae, lipooligosaccharide, and adenylate cyclase toxin. the disease causes a worldwide threat to public health despite a high vaccination coverage. the course of whooping cough in adults is frequently atypical, causing difficulty in diagnosis. in this report we present five patients hospi ... | 2017 | 28039663 |
| camp signaling of adenylate cyclase toxin blocks the oxidative burst of neutrophils through epac-mediated inhibition of phospholipase c activity. | the adenylate cyclase toxin-hemolysin (cyaa) plays a key role in immune evasion and virulence of the whooping cough agent bordetella pertussis. cyaa penetrates the complement receptor 3-expressing phagocytes and ablates their bactericidal capacities by catalyzing unregulated conversion of cytosolic atp to the key second messenger molecule camp. we show that signaling of cyaa-generated camp blocks the oxidative burst capacity of neutrophils by two converging mechanisms. one involves camp/protein ... | 2017 | 28039302 |
| risk factors associated with bordetella pertussis among infants aged ≤4 months in the pre-tdap era - united states, 2002-2005. | in the united states, infants have the highest reported pertussis incidence and death rates. improved understanding of infant risk factors is needed to optimize prevention strategies. | 2016 | 28033240 |
| immunogenicity and safety of 10-valent pneumococcal non-typeable haemophilus influenzae protein d conjugate vaccine (phid-cv) administered to children with sickle cell disease between 8 weeks and 2 years of age: a phase iii, open, controlled study. | immunogenicity, safety and reactogenicity of the 10-valent pneumococcal non-typeable haemophilus influenzae protein d conjugate vaccine (phid-cv) were evaluated in children with sickle cell disease (scd), who are at increased risk for infections. | 2016 | 28030521 |
| acylation of the bordetella pertussis cyaa-hemolysin: functional implications for efficient membrane insertion and pore formation. | previously, the ~130-kda cyaa-hemolysin domain (cyaa-hly) from bordetella pertussis co-expressed with cyac-acyltransferase in escherichia coli was demonstrated to be palmitoylated at lys(983) and thus activated its hemolytic activity against target erythrocytes. here, we report the functional importance of lys(983)-palmitoylation for membrane insertion and pore formation of cyaa-hly. intrinsic fluorescence emissions of both non-acylated cyaa-hly (na/cyaa-hly) and cyaa-hly were indistinguishable, ... | 2017 | 27993565 |
| proteome analysis is a valuable tool to monitor antigen expression during upstream processing of whole-cell pertussis vaccines. | physicochemical and immunochemical assays were applied to substantiate the relation between upstream processing and the quality of whole-cell pertussis vaccines. bordetella pertussis bacteria were cultured on a chemically defined medium using a continuous cultivation process in stirred tank reactors to obtain uniform protein expression. continuous culture favors the consistent production of proteins known as virulence factors. magnesium sulfate was added during the steady state of the culture in ... | 2017 | 27977922 |
| evaluation of a commercial loop-mediated isothermal amplification assay for diagnosis of bordetella pertussis infection. | we evaluated a commercial loop-mediated isothermal amplification (lamp) assay kit for bordetella pertussis detection. the lamp primers were designed to target the ptxp1 allele of the pertussis toxin promoter, but the assay could detect b. pertussis ptxp3 and ptxp8 strains in addition to ptxp1 strains, with high analytical sensitivity. | 2017 | 27974227 |
| antimicrobial susceptibility and molecular detection of pertactin-producing and pertactin-deficient bordetella pertussis. | resurgence of bordetella pertussis in recent years in the united states has coincided with a dramatic rise in pertactin-deficient strains. limited data exist on detectability by nucleic acid amplification testing and antimicrobial susceptibility of pertactin-deficient b. pertussis. this study compares 15 pertactin-producing and 15 pertactin-deficient b. pertussis isolates. pertactin-producing and pertactin-deficient strains were equally detected by nucleic acid amplification testing and were sus ... | 2017 | 27956730 |
| oxygen and bis(3',5')-cyclic dimeric guanosine monophosphate binding control oligomerization state equilibria of diguanylate cyclase-containing globin coupled sensors. | bacteria sense their environment to alter phenotypes, including biofilm formation, to survive changing conditions. heme proteins play important roles in sensing the bacterial gaseous environment and controlling the switch between motile and sessile (biofilm) states. globin coupled sensors (gcs), a family of heme proteins consisting of a globin domain linked by a central domain to an output domain, are often found with diguanylate cyclase output domains that synthesize c-di-gmp, a major regulator ... | 2016 | 27933792 |
| drivers of airborne human-to-human pathogen transmission. | airborne pathogens - either transmitted via aerosol or droplets - include a wide variety of highly infectious and dangerous microbes such as variola virus, measles virus, influenza a viruses, mycobacterium tuberculosis, streptococcus pneumoniae, and bordetella pertussis. emerging zoonotic pathogens, for example, mers coronavirus, avian influenza viruses, coxiella, and francisella, would have pandemic potential were they to acquire efficient human-to-human transmissibility. here, we synthesize in ... | 2017 | 27918958 |
| whole-genome sequencing and comparative genomic analysis of bordetella pertussis isolates from the 2007-2008 epidemic in israel. | | 2017 | 27914992 |
| pertussis in north-central and northwestern regions of algeria. | pertussis outbreaks continue to occur in many countries despite high vaccination coverage. under-diagnosed cases in adolescents and adults may result in increased transmission to infants, who are at risk of severe pertussis. additional measures to protect both groups should be considered. | 2016 | 27886031 |
| does breastfeeding protect young infants from pertussis? case-control study and immunologic evaluation. | pertussis infection can be severe in unvaccinated infants. a case-control study was conducted to investigate the potential role of breastfeeding in protecting young, unvaccinated infants from pertussis. | 2017 | 27870812 |
| [trends in pertussis mortality and morbidity in colombia, 2002-2012]. | whooping cough is a public health problem that mainly affects children under one year of age with highly lethal outcomes. it is a re-emerging disease, which is preventable by immunization. objective: to analyze mortality and morbidity trends of whooping cough in colombia between 2002 and 2012. materials and methods: we conducted a quantitative descriptive study of deaths by pertussis between 2002 and 2012 using data from death certificates registered by the departamento administrativo nacional d ... | 2016 | 27869387 |
| il-17-producing innate and pathogen-specific tissue resident memory γδ t cells expand in the lungs of bordetella pertussis-infected mice. | γδ t cells play a role in protective immunity to infection at mucosal surface, but also mediate pathology in certain autoimmune diseases through innate il-17 production. recent reports have suggested that γδ t cells can have memory analogous to conventional αβ t cells. in this study we have examined the role of γδ t cells in immunity to the respiratory pathogen bordetella pertussis γδ t cells, predominantly vγ4(-)γ1(-) cells, produced il-17 in the lungs as early as 2 h after infection. the bacte ... | 2017 | 27864475 |
| hospital costs of bordetella pertussis in new zealand children. | to estimate hospitalisation costs for children with pertussis in new zealand. | 2016 | 27857241 |
| modulation of pertussis and adenylate cyclase toxins by sigma factor rpoe in bordetella pertussis. | bordetella pertussis is a human pathogen that can infect the respiratory tract and cause the disease known as whooping cough. b. pertussis uses pertussis toxin (pt) and adenylate cyclase toxin (act) to kill and modulate host cells to allow the pathogen to survive and persist. b. pertussis encodes many uncharacterized transcription factors, and very little is known about their functions. rpoe is a sigma factor which, in other bacteria, responds to oxidative, heat, and other environmental stresses ... | 2017 | 27849178 |
| reduction of pertussis inflammatory pathology by therapeutic treatment with sphingosine-1-phosphate receptor ligands by a pertussis toxin-insensitive mechanism. | recent data have demonstrated the potential of sphingosine 1-phosphate (s1p) receptor (s1pr) agonism in the treatment of infectious diseases. a previous study used a murine model of bordetella pertussis infection to demonstrate that treatment with the s1pr agonist aal-r reduces pulmonary inflammation during infection. in the current study, we showed that this effect is mediated via the s1pr1 on lysm+ (myeloid) cells. signaling via this receptor results in reduced lung inflammation and cellular r ... | 2017 | 27815382 |
| identifying children at risk of malignant bordetella pertussis infection. | to identify factors associated with malignant pertussis. | 2017 | 27811532 |
| bordetella adenylate cyclase toxin interacts with filamentous haemagglutinin to inhibit biofilm formation in vitro. | bordetella pertussis, the causative agent of whooping cough, secretes and releases adenylate cyclase toxin (act), which is a protein bacterial toxin that targets host cells and disarms immune defenses. act binds filamentous haemagglutinin (fha), a surface-displayed adhesin, and until now, the consequences of this interaction were unknown. a b. bronchiseptica mutant lacking act produced more biofilm than the parental strain; leading irie et al. to propose the act-fha interaction could be responsi ... | 2017 | 27731909 |
| pneumomediastinum as a complication of critical pertussis. | pertussis is a common and potentially serious disease affecting mainly infants and young children. in its non-classic presentation, pertussis can be clinically indistinguishable from other respiratory illnesses. pertussis today often remains underdiagnosed in adults. our aims was to report a complicated cases of pertussis. | 2016 | 25764484 |
| aetiology and risk factors of community-acquired pneumonia in hospitalized patients in norway. | in norway, data on the aetiology of community-acquired pneumonia (cap) in hospitalized patients are limited. the aims of this study were to investigate the bacterial aetiology of cap in hospitalized patients in norway, risk factors for cap and possible differences in risk factors between patients with legionnaire's disease and pneumonia because of other causes. | 2016 | 25764275 |
| modeling the effects of priming with the whole-cell bordetella pertussis vaccine-reply. | | 2016 | 27802485 |
| modeling the effects of priming with the whole-cell bordetella pertussis vaccine. | | 2016 | 27802457 |
| use of a toxin neutralization assay to characterize the serologic response to adenylate cyclase toxin after infection with bordetella pertussis. | adenylate cyclase toxin (act) is an essential virulence factor of bordetella pertussis, and antibodies to act protect against b. pertussis infection in mice. the toxin is therefore a strong candidate antigen for addition to future acellular pertussis vaccines. in order to characterize the functionality of the immunologic response to act after infection, we developed an assay for testing the ability of serum samples from subjects infected with b. pertussis to neutralize act-induced cytotoxicity i ... | 2017 | 27760780 |