| detection of aspergillus fumigatus pulmonary fungal infections in mice with (99m)tc-labeled morf oligomers targeting ribosomal rna. | invasive aspergillosis is a major cause of infectious morbidity and mortality in immunocompromised patients. the fungus aspergillus fumigatus (a. fumigatus) is the primary causative agent of invasive aspergillosis. however, a. fumigatus infections remain difficult to diagnose particularly in the early stages due to the lack of a rapid, sensitive and specific diagnostic approach. in this study, we investigated (99m)tc labeled morf oligomers targeting fungal ribosomal rna (rrna) for the imaging de ... | 2013 | 23142409 |
| the effect of lipoprotein-associated phospholipase a2 deficiency on pulmonary allergic responses in aspergillus fumigatus sensitized mice. | lipoprotein-associated phospholipase a2 (lp-pla2)/platelet-activating factor acetylhydrolase (paf-ah) has been implicated in the pathogenesis of cardiovascular disease. a therapeutic targeting of this enzyme was challenged by the concern that increased circulating platelet activating factor (paf) may predispose to or increase the severity of the allergic airway response. the aim of this study was to investigate whether lp-pla2 gene deficiency increases the risk of paf and ige-mediated inflammato ... | 2012 | 23140447 |
| biosynthesis of the fungal cell wall polysaccharide galactomannan requires intraluminal gdp-mannose. | fungal cell walls frequently contain a polymer of mannose and galactose called galactomannan. in the pathogenic filamentous fungus aspergillus fumigatus, this polysaccharide is made of a linear mannan backbone with side chains of galactofuran and is anchored to the plasma membrane via a glycosylphosphatidylinositol or is covalently linked to the cell wall. to date, the biosynthesis and significance of this polysaccharide are unknown. the present data demonstrate that deletion of the golgi udp-ga ... | 2012 | 23139423 |
| some southern african plant species used to treat helminth infections in ethnoveterinary medicine have excellent antifungal activities. | diseases caused by microorganisms and parasites remain a major challenge globally and particularly in sub-saharan africa to man and livestock. resistance to available antimicrobials and the high cost or unavailability of antimicrobials complicates matters. many rural people use plants to treat these infections. because some anthelmintics e.g. benzimidazoles also have good antifungal activity we examined the antifungal activity of extracts of 13 plant species used in southern africa to treat gast ... | 2012 | 23134805 |
| fungal infections associated with contaminated methylprednisolone in tennessee. | we investigated an outbreak of fungal infections of the central nervous system that occurred among patients who received epidural or paraspinal glucocorticoid injections of preservative-free methylprednisolone acetate prepared by a single compounding pharmacy. | 2012 | 23131029 |
| efficacy and pharmacodynamics of voriconazole combined with anidulafungin in azole-resistant invasive aspergillosis. | azole resistance is an emerging problem in the treatment of aspergillus fumigatus infections. combination therapy may be an alternative approach to improve therapeutic outcome in azole-resistant invasive aspergillosis (ia). the in vivo efficacy of voriconazole and anidulafungin was investigated in a non-neutropenic murine model of ia using voriconazole-susceptible and voriconazole-resistant a. fumigatus clinical isolates. | 2013 | 23129729 |
| differential proteomic analysis of aspergillus fumigatus morphotypes reveals putative drug targets. | aspergillus fumigatus is the main etiological agent of invasive aspergillosis, an important opportunistic infection for neutropenic patients. the main risk groups are patients with acute leukemia and bone marrow transplantation recipients. the lack of an early diagnostic test together with the limited spectrum of antifungal drugs remains a setback to the successful treatment of this disease. during invasive infection the inhaled fungal conidia enter the morphogenic cycle leading to angioinvasive ... | 2013 | 23128298 |
| stimulation of suicidal erythrocyte death by fumagillin. | fumagillin, a cyclohexane isolated from fungus aspergillus fumigatus, has anti-infective and anti-cancer potency. fumagillin is at least partially effective by inducing suicidal death or apoptosis. in analogy to apoptosis of nucleated cells, eryptosis is the suicidal death of erythrocytes characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine exposure at the cell surface. stimulators of eryptosis include increase of cytosolic ca(2+)-activity ([ca(2+)](i)) and cerami ... | 2013 | 23121865 |
| pharmacodynamics of anidulafungin against clinical aspergillus fumigatus isolates in a nonneutropenic murine model of disseminated aspergillosis. | azole resistance is an emerging increasing problem in aspergillus fumigatus that results in treatment failure. alternative treatments may improve the therapeutic outcome in patients with azole-resistant invasive aspergillosis (ia). little is known about the in vivo efficacy of the echinocandin anidulafungin (afg) in ia. the in vivo efficacy of 2.5, 5, 10, and 20 mg/kg of body weight afg was assessed against two clinical aspergillus fumigatus isolates with identical afg minimum effective concentr ... | 2013 | 23114773 |
| interaction of platelets and anidulafungin against aspergillus fumigatus. | the combination of platelets and anidulafungin at 0.03 μg/ml significantly (p < 0.05) reduced the germination rate and hyphal elongation in aspergillus fumigatus compared to those with either anidulafungin only or an untreated control. platelets decreased the expression of the fks gene, which plays an important role in cell wall synthesis. our results suggest that human platelets plus anidulafungin might contribute to defense against a. fumigatus. | 2013 | 23114752 |
| a controlled study to determine the efficacy of loxostylis alata (anacardiaceae) in the treatment of aspergillus in a chicken (gallus domesticus) model in comparison to ketoconazole. | the poultry industry due to intensive methods of farming is burdened with losses from numerous infectious agents, of which one is the fungus aspergillus fumigatus. in a preliminary study, the extracts of loxostylis alata a. spreng, ex rchb. showed good activity in vitro against a. fumigatus with a minimum inhibitory concentration of 0.07 mg/ml. for this study crude, a crude acetone extract of l. alata leaves was evaluated for its acute toxicity in a healthy chicken model and for efficacy in an i ... | 2012 | 23114185 |
| synthesis and antimicrobial activity of some novel cross-linked chitosan hydrogels. | four novel hydrogels based on chitosan were synthesized via a cross-linking reaction of chitosan with different concentrations of oxalyl bis 4-(2,5-dioxo-2h-pyrrol- 1(5h)-yl)benzamide. their structures were confirmed by fourier transform infrared x-ray (ftir), scanning electron microscopy (sem) and x-ray diffraction. the antimicrobial activities of the hydrogels against two crop-threatening pathogenic fungi namely: aspergillus fumigatus (a. fumigatus, rcmba 06002), and aspergillus niger (a. nige ... | 2012 | 23109847 |
| successful treatment of liver aspergilloma by caspofungin acetate first-line therapy in a non-immunocompromised patient. | aspergillosis remains to be a life-threatening complication in immunocompromised patients. however, aspergillus infection can be observed in non-immunocompromised individuals in rare cases. we report a case of liver aspergilloma in a chronic aplastic anemia patient under relatively intact immune status. therapeutic strategy for this rare condition was extensively discussed and caspofungin acetate single agent first-line therapy was applied after careful consideration. encouraging clinical and ra ... | 2012 | 23109838 |
| triazole fungicides and the selection of resistance to medical triazoles in the opportunistic mould aspergillus fumigatus. | azole resistance is an emerging problem in the opportunistic mould aspergillus fumigatus. the triazoles are the most important agents for the management of aspergillus diseases in humans. selection for acquired resistance may occur in the hospital setting through exposure to high doses of azoles during azole therapy, but evidence is accumulating that a. fumigatus may become resistant to medical triazoles through environmental exposure to fungicides. the recovery of a. fumigatus isolates resistan ... | 2013 | 23109245 |
| dsc orthologs are required for hypoxia adaptation, triazole drug responses, and fungal virulence in aspergillus fumigatus. | hypoxia is an environmental stress encountered by aspergillus fumigatus during invasive pulmonary aspergillosis (ipa). the ability of this mold to adapt to hypoxia is important for fungal virulence and genetically regulated in part by the sterol regulatory element binding protein (srebp) srba. srba is required for fungal growth in the murine lung and to ultimately cause lethal disease in murine models of ipa. here we identified and partially characterized four genes (dsca, dscb, dscc, and dscd, ... | 2012 | 23104569 |
| invasive aspergillosis: drug-dispensing systems as a source of filamentous fungal contamination in high-risk units? | high-risk units with air-control measures at grenoble university hospital are equipped with automated dispensing systems that are filled daily using drug trolleys routed from the pharmacy to the ward. the aim of this study was to evaluate the level of filamentous fungi (ff) contamination present in trolleys under usual conditions and after cleaning with aniosurf(®) (fungicidal disinfectant). ff were detected in all samples, and 83.3% of samples were contaminated with aspergillus fumigatus. clean ... | 2012 | 23102819 |
| bronchial colonisation in patients with lung cancer: a prospective study. | bronchial colonisation is frequently reported in patients with lung cancer, and has a potential impact on therapeutic management and prognosis. we aimed to prospectively define the prevalence and nature of bronchial colonisation in patients at the time of diagnosing lung cancer. 210 consecutive patients with lung cancer underwent a flexible bronchoscopy for lung cancer. the type and frequency of bacterial, mycobacterial and fungal colonisation were analysed and correlated with the patients' and ... | 2013 | 23100491 |
| [frequency of pulmonary mycoses determined by analyzing lung secretion samples]. | the frequency of pulmonary mycoses has increased in the past few years specially in immunocompromised patients. | 2012 | 23096664 |
| breaking the regioselectivity of indole prenyltransferases: identification of regular c3-prenylated hexahydropyrrolo[2,3-b]indoles as side products of the regular c2-prenyltransferase ftmpt1. | the prenyltransferase ftmpt1 from aspergillus fumigatus is involved in the biosynthesis of fumitremorgin-type alkaloids and catalysed the regular c2-prenylation of brevianamide f (cyclo-l-trp-l-pro). it has been shown that ftmpt1 also accepted a number of other tryptophan-containing cyclic dipeptides and prenylated them, in the presence of dimethylallyl diphosphate, at c-2 of the indole nucleus. detailed analysis of the incubation mixtures of ftmpt1 with these cyclic dipeptides revealed the pres ... | 2012 | 23090579 |
| in vivo molecular microimaging of pulmonary aspergillosis. | the early diagnosis of invasive pulmonary aspergillosis (ipa) is challenging. fibered confocal fluorescence microscopy (fcfm) is a new technique that allows in vivo imaging of the lung microstructure during bronchoscopy. in this study, we investigated the ability of fcfm to detect a fluorescent peptide-tracer bound to aspergillus fumigatus in experimental ipa in 13 immunosuppressed, non-neutropenic rats. subpleural ipa microabscesses were imaged through a transthoracic window using fcfm in vivo ... | 2013 | 23088299 |
| vea regulates conidiation, gliotoxin production, and protease activity in the opportunistic human pathogen aspergillus fumigatus. | invasive aspergillosis by aspergillus fumigatus is a leading cause of infection-related mortality in immunocompromised patients. in this study, we show that vea, a major conserved regulatory gene that is unique to fungi, is necessary for normal morphogenesis in this medically relevant fungus. although deletion of vea results in a strain with reduced conidiation, overexpression of this gene further reduced conidial production, indicating that vea has a major role as a regulator of development in ... | 2012 | 23087369 |
| successful resumption of peritoneal dialysis after aspergillus fumigatus peritonitis. | | 2012 | 23084928 |
| the function and evolution of the aspergillus genome. | species in the filamentous fungal genus aspergillus display a wide diversity of lifestyles and are of great importance to humans. the decoding of genome sequences from a dozen species that vary widely in their degree of evolutionary affinity has galvanized studies of the function and evolution of the aspergillus genome in clinical, industrial, and agricultural environments. here, we synthesize recent key findings that shed light on the architecture of the aspergillus genome, on the molecular fou ... | 2013 | 23084572 |
| the index case for the fungal meningitis outbreak in the united states. | persistent neutrophilic meningitis presents a diagnostic challenge, because the differential diagnosis is broad and includes atypical infectious causes. we describe a case of persistent neutrophilic meningitis due to aspergillus fumigatus in an immunocompetent man who had no evidence of sinopulmonary or cutaneous disease. an epidural glucocorticoid injection was identified as a potential route of entry for this organism into the central nervous system, and the case was reported to the state heal ... | 2012 | 23083311 |
| aspergillus nidulans galactofuranose biosynthesis affects antifungal drug sensitivity. | the cell wall is essential for fungal survival in natural environments. many fungal wall carbohydrates are absent from humans, so they are a promising source of antifungal drug targets. galactofuranose (galf) is a sugar that decorates certain carbohydrates and lipids. it comprises about 5% of the aspergillus fumigatus cell wall, and may play a role in systemic aspergillosis. we are studying aspergillus wall formation in the tractable model system, a. nidulans. previously we showed single-gene de ... | 2012 | 23078837 |
| multistate outbreak of fungal infection associated with injection of methylprednisolone acetate solution from a single compounding pharmacy - united states, 2012. | on september 18, 2012, the tennessee department of health was alerted by a clinician regarding a patient with culture-confirmed aspergillus fumigatus meningitis diagnosed 46 days after epidural steroid injection at a tennessee ambulatory surgical center. by september 27, the initial investigation, carried out by the tennessee department of health in collaboration with cdc and the north carolina department of health and human services, had identified an additional eight patients with clinically d ... | 2012 | 23076093 |
| bacterial inactivation by a singlet oxygen bubbler: identifying factors controlling the toxicity of (1)o2 bubbles. | a microphotoreactor device was developed to generate bubbles (1.4 mm diameter, 90 μl) containing singlet oxygen at levels toxic to bacteria and fungus. as singlet oxygen decays rapidly to triplet oxygen, the bubbles leave behind no waste or byproducts other than o(2). from a comparative study in deaerated, air saturated, and oxygenated solutions, it was reasoned that the singlet oxygen bubbles inactivate escherichia coli and aspergillus fumigatus, mainly by an oxygen gradient inside and outside ... | 2012 | 23075418 |
| interaction of the pathogenic mold aspergillus fumigatus with lung epithelial cells. | aspergillus fumigatus is an opportunistic environmental mold that can cause severe allergic responses in atopic individuals and poses a life-threatening risk for severely immunocompromised patients. infection is caused by inhalation of fungal spores (conidia) into the lungs. the initial point of contact between the fungus and the host is a monolayer of lung epithelial cells. understanding how these cells react to fungal contact is crucial to elucidating the pathobiology of aspergillus-related di ... | 2012 | 23055997 |
| expression and characterization of a novel enantioselective lipase from aspergillus fumigatus. | a 1,080-bp cdna (cgmcc 2873) encoding of a cold-active lipase of aspergillus fumigatus (afl67) was cloned and expressed in escherichia coli for the first time. the new lipase, afl67, was one-step purified by 8.30 folds through ni-nta affinity chromatography with a recovery of 86.8 %. the specific activity of purified afl67 was 449 u mg(-1) on p-np hexanoate. afl67 preferentially hydrolyzed p-nitrophenyl esters of short- and medium-chain fatty acids, with p-nitrophenyl hexanoate the maximum. the ... | 2012 | 23054817 |
| optimization of decolorization of palm oil mill effluent (pome) by growing cultures of aspergillus fumigatus using response surface methodology. | the conventional treatment process of palm oil mill effluent (pome) produces a highly colored effluent. colored compounds in pome cause reduction in photosynthetic activities, produce carcinogenic by-products in drinking water, chelate with metal ions, and are toxic to aquatic biota. thus, failure of conventional treatment methods to decolorize pome has become an important problem to be addressed as color has emerged as a critical water quality parameter for many countries such as malaysia. aspe ... | 2013 | 23054764 |
| aspergillus fumigatus and other thermophilic fungi in nests of wetland birds. | a study was performed on the numbers and species diversity of thermophilic fungi (growing at 45 °c in vitro) in 38 nests of 9 species of wetland birds, taking into account the physicochemical properties of the nests and the bird species. it was found that in nests with the maximum weight (nests of mute swan), the number and diversity of thermophilic fungi were significantly greater than in other nests, with lower weight. the diversity of the thermophilic biota was positively correlated with the ... | 2013 | 23054328 |
| synthesis, characterization, biological evaluation and qsar studies of 11-p-substituted phenyl-12-phenyl-11a,12-dihydro-11h-indeno[2,1-c][1,5]benzothiazepines as potential antimicrobial agents. | a novel series of 11-p-substituted phenyl-12-phenyl-11a,12-dihydro-11h-indeno[2,1-c][1,5]benzothiazepines (3) has been synthesized and screened for their antimicrobial activity against gram-positive (bacillus subtilis and staphylococcus aureus) and gram-negative (escherichia coli and pseudomonas aeruginosa) bacteria and fungi (aspergillus fumigates and candida albicans). the antimicrobial evaluation data indicated that the compounds, 3d, 3g, 3h, 3k-3p exhibited very promising antibacterial activ ... | 2012 | 23047232 |
| superoxide scavenging and antimicrobial activities of novel transition metal complexes of oxydiacetate dianion as primary ligand: spectral characterization, cyclic voltammetric investigations and crystal structure. | complexes of stoichiometries [m(oda)(4-pic)h(2)o]·xh(2)o [m = cu(1), x = 2; co (2), x = 4; ni (3), x = 2; ] [fe(oda)(4-pic)]·cl (4) and {[cu(2-pa)(2)]·2h(2)o}(n) (5) (h(2)oda = oxydiacetic acid, 4-pic = 4-picoline and 2-pah = 2-picolinic acid) were prepared to explore their uses as possible models for mono- and poly-nuclear metallo-pharmaceuticals. the chemical structures were elucidated by ir, fab-mass, (1)h, (13)c nmr, epr and uv-visible (ligand field) spectral analyses. electrochemical invest ... | 2012 | 23047227 |
| antifungal spectrum, in vivo efficacy, and structure-activity relationship of ilicicolin h. | ilicicolin h is a polyketide-nonribosomal peptide synthase (nrps)-natural product isolated from gliocadium roseum, which exhibits potent and broad spectrum antifungal activity, with sub-μg/ml mics against candida spp., aspergillus fumigatus, and cryptococcus spp. it showed a novel mode of action, potent inhibition (ic50 = 2-3 ng/ml) of the mitochondrial cytochrome bc1 reductase, and over 1000-fold selectivity relative to rat liver cytochrome bc1 reductase. ilicicolin h exhibited in vivo efficacy ... | 2012 | 24900384 |
| method for identifying heat-resistant fungi of the genus neosartorya. | species of the genus neosartorya are heat-resistant fungi that cause the spoilage of heat-processed acidic foods due to the formation of heat-resistant ascospores, and they produce mycotoxins, such as fumitremorgins and gliotoxin. their anamorphs are phylogenetically and morphologically very close to aspergillus fumigatus, which has never been reported as a spoilage agent in heat-processed food products. therefore it is important to discriminate between the species of neosartorya and a. fumigatu ... | 2012 | 23043829 |
| identification of the nad(p)h binding site of eukaryotic udp-galactopyranose mutase. | udp-galactopyranose mutase (ugm) plays an essential role in galactofuranose biosynthesis in microorganisms by catalyzing the conversion of udp-galactopyranose to udp-galactofuranose. the enzyme has gained attention recently as a promising target for the design of new antifungal, antitrypanosomal, and antileishmanial agents. here we report the first crystal structure of ugm complexed with its redox partner nad(p)h. kinetic protein crystallography was used to obtain structures of oxidized aspergil ... | 2012 | 23036087 |
| comparison of transcriptome technologies in the pathogenic fungus aspergillus fumigatus reveals novel insights into the genome and mpka dependent gene expression. | the filamentous fungus aspergillus fumigatus has become the most important airborne fungal pathogen causing life-threatening infections in immuno-compromised patients. recently developed high-throughput transcriptome and proteome technologies, such as microarrays, rna deep-sequencing, and lc-ms/ms of peptide mixtures, are of enormous value for systematically investigating pathogenic organisms. in the field of infection biology, one of the priorities is to collect and standardise data, in order t ... | 2012 | 23031507 |
| evaluation of serum galactomannan detection for diagnosis of feline upper respiratory tract aspergillosis. | measurement of serum galactomannan (gm), a polysaccharide fungal cell-wall component, is a non-invasive test for early diagnosis of invasive aspergillosis in humans. feline upper respiratory tract (urt) aspergillosis is an emerging infectious disease in cats. diagnosis requires biopsy for procurement of tissue specimens for cytological or histological detection of fungal hyphae and for fungal culture. the aim of this study was to evaluate serum gm measurement as a non-invasive diagnostic test fo ... | 2013 | 23031342 |
| differential strain-related tissue immune response to sublethal systemic aspergillus fumigatus infection in mice. | using a nonlethal systemic aspergillus fumigatus infection, we have recently shown that similarly efficient elimination of fungus from spleens of prototypic th1 (c57bl/6) and prototypic th2 (balb/c) mice is associated with differential immune responses. in light of these data and given the disseminated character of infection, the aim of the present study is to explore whether there are also strain-dependent differences in antifungal responses in peripheral tissues of infected mice. although simi ... | 2013 | 23030850 |
| detection of biofilm-grown aspergillus fumigatus by means of atomic force spectroscopy: ultrastructural effects of alginate lyase. | aspergillus fumigatus has become a leading cause of fungal morbidity and mortality, especially in immunocompromised patients. this fungus is able to grow as a multicellular community and produce a hydrophobic extracellular matrix (ecm), mainly composed of galactomannan and α-1,3 glucans, to protect itself from host defenses and antimicrobial drugs. this matrix envelops the fungus hyphae, binding them into a contiguous sheath on the colony surface, forming a biofilm and increasing the fungal resi ... | 2012 | 23026204 |
| protective effector cells of the recombinant asp f3 anti-aspergillosis vaccine. | an aspergillus fumigatus vaccine based on recombinant asp f3-protein has the potential to prevent aspergillosis in humans, a devastating fungal disease that is the prime obstacle to the success of hematopoietic cell transplantation. this vaccine protects cortisone acetate (ca)-immunosuppressed mice from invasive pulmonary aspergillosis via cd4(+) t cell mediators. aside from these mediators, the nature of downstream fungicidal effectors is not well understood. neutrophils and macrophages protect ... | 2012 | 23024640 |
| nosa, a transcription factor important in aspergillus fumigatus stress and developmental response, rescues the germination defect of a laea deletion. | aspergillus fumigatus is an increasingly serious pathogen of immunocompromised patients, causing the often fatal disease invasive aspergillosis (ia). one a. fumigatus virulence determinant of ia is laea, a conserved virulence factor in pathogenic fungi. to further understand the role of laea in ia, the expression profile of δlaea was compared to wild type, and several transcription factors were found significantly misregulated by laea loss. one of the transcription factors up-regulated over 4-fo ... | 2012 | 23022264 |
| unraveling the antifungal activity of a south american rattlesnake toxin crotamine. | crotamine is a highly basic peptide from the venom of crotalus durissus terrificus rattlesnake. its common gene ancestry and structural similarity with the β-defensins, mainly due to an identical disulfide bond pattern, stimulated us to assess the antimicrobial properties of native, recombinant, and chemically synthesized crotamine. antimicrobial activities against standard strains and clinical isolates were analyzed by the colorimetric microdilution method showing a weak antibacterial activity ... | 2013 | 23022146 |
| occurrence of household mold and efficacy of sodium hypochlorite disinfectant. | the occurrence and distribution of mold on household surfaces and the efficacy of bleach-based (sodium hypochlorite, naocl) disinfectants on mold viability and allergenicity was documented. household microenvironments prone to increased moisture were specifically targeted. using the sticky tape method, 1330 samples were collected from non-porous indoor surfaces of 160 homes across the united states, and analyzed for mold. homes were randomly selected and recruited via phone interviews. culture a ... | 2012 | 23016564 |
| reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation. | reaginic antibodies (ige and some igg subclasses) and mast cells play important roles in the induction of type i immediate hypersensitivity reactions. these antibodies bind through their fc fragment to high affinity receptors (fcεri) present in the membrane of mast cells and basophils. the cross-linking of the receptor initiates a coordinated sequence of biochemical and morphological events that results in exocytosis of secretory granules containing pre-formed inflammatory mediators, secretion o ... | 2012 | 23011757 |
| loss of caveolin-1 from bronchial epithelial cells and monocytes in human subjects with asthma. | caveolin-1 has emerged as a critical regulator of signaling pathways involved in lung fibrosis and inflammation. | 2012 | 23004679 |
| the putative guanine nucleotide exchange factor rica mediates upstream signaling for growth and development in aspergillus. | heterotrimeric g proteins (g proteins) govern growth, development, and secondary metabolism in various fungi. here, we characterized rica, which encodes a putative gdp/gtp exchange factor for g proteins in the model fungus aspergillus nidulans and the opportunistic human pathogen aspergillus fumigatus. in both species, rica mrna accumulates during vegetative growth and early developmental phases, but it is not present in spores. the deletion of rica results in severely impaired colony growth and ... | 2012 | 23002107 |
| efficacy of liposomal amphotericin b for prophylaxis of acute or reactivation models of invasive pulmonary aspergillosis. | the efficacy of antifungal prophylaxis for prevention of invasive aspergillosis (ia) may depend on whether ia results from recent inhalation of spores or reactivation of latent colonisation. compare the efficacy of liposomal amphotericin b (lamb) for prophylaxis in acute and reactivation models of ia. in the acute model, mice immunosuppressed from day 0 were challenged at day 3 with an aerosol of aspergillus fumigatus. lamb (15 mg kg(-1) ) was administered at day 0 or at challenge. in the reacti ... | 2013 | 22998025 |
| development and application of mold antigen-specific enzyme-linked immunosorbent assays (elisa) to quantify airborne antigen exposure. | the aim of our study was to develop specific enzyme-linked immunosorbent assays (elisas) and apply these to assess mold antigen exposure in composting plants. sandwich elisas based on polyclonal antibodies to aspergillus fumigatus (af), penicillium chrysogenum (pc), and cladosporium herbarum (ch) antigens were developed and validated. reactivity to 18 different mold species was tested. to optimize extraction procedure, inhalable dust samples taken by a parallel sampler were extracted with or wit ... | 2012 | 22994572 |
| fatal outcome after heart transplantation caused by aspergillus lentulus. | opportunistic invasive fungal infections are a major cause of mortality in immunocompromised patients. early diagnosis of invasive aspergillosis and proper identification of the causative agent is crucial for guidance of therapy. accurate differentiation of aspergillus lentulus, a filamentous fungus often misidentified as atypical aspergillus fumigatus, is of concern as multiple antifungal drugs show a reduced susceptibility. this is the first report, to our knowledge, of a proven pulmonary inva ... | 2012 | 22988985 |
| breakthrough pulmonary aspergillus fumigatus infection with multiple triazole resistance in a spanish patient with chronic myeloid leukemia. | an allogeneic hematopoietic cell transplantation (allo-hct) patient presented with chronic pulmonary aspergillosis associated to pulmonary graft versus host disease (gvhd) and was treated for a long time with several antifungal agents that were administered as prophylaxis, combination therapies, and maintenance treatment. the patient suffered from a breakthrough invasive pulmonary aspergillosis due to aspergillus fumigatus after long-term antifungal therapy. | 2013 | 22986228 |
| effect of allergy and inflammation on eicosanoid gene expression in cftr deficiency. | allergic bronchopulmonary aspergillosis (abpa) is a complicating factor in cystic fibrosis (cf), affecting 2-15% of patients. we hypothesized that sensitization/challenge of cftr(-/-) mice with an aspergillus fumigatus (af) extract will affect eicosanoid pathway gene expression, impacting abpa and cf. | 2013 | 22985691 |
| azole-resistant invasive aspergillosis in a patient with acute myeloid leukaemia in germany. | we report the first culture-proven case of invasive aspergillosis (ia) caused by azole-resistant aspergillus fumigatus in a patient with acute myeloid leukaemia in germany. ia presented as breakthrough infection under posaconazole prophylaxis. analysis of the resistance mechanism revealed the tr/l98h mutation in the cyp51a gene, which indicates an environmental origin of the strain. this case underscores the need for monitoring azole resistance in aspergillus spp. and for routine susceptibility ... | 2012 | 22971327 |
| what is the current place of azoles in allergic bronchopulmonary aspergillosis and severe asthma with fungal sensitization. | evaluation of: chishimba l, niven rm, cooley j, denning dw. voriconazole and posaconazole improve asthma severity in allergic bronchopulmonary aspergillosis and severe asthma with fungal sensitization. j. asthma 49(4), 423-433 (2012). allergic bronchopulmonary aspergillosis (abpa) is a pulmonary disorder caused by hypersensitivity to aspergillus fumigatus that usually complicates the disease course of patients with asthma and cystic fibrosis. oral corticosteroids are currently the treatment of c ... | 2012 | 22971061 |
| characterization of the velvet regulators in aspergillus fumigatus. | fungal development and secondary metabolism is intimately associated via activities of the fungi-specific velvet family proteins. here we characterize the four velvet regulators in the opportunistic human pathogen aspergillus fumigatus. the deletion of afuvosa, afuvea and afuvelb causes hyperactive asexual development (conidiation) and precocious and elevated accumulation of afubrla during developmental progression. moreover, the absence of afuvosa, afuvea or afuvelb results in the abundant form ... | 2012 | 22970834 |
| effects of aspergillus fumigatus colonization on lung function in cystic fibrosis. | aspergillus fumigatus is frequently isolated from cystic fibrosis (cf) patients and is notorious for its role in the debilitating condition of allergic bronchopulmonary aspergillosis (abpa). although cf patients suffer from perpetual microorganism-related lung disease, it is unclear whether a. fumigatus colonization has a role in causing accelerated lung function decline and whether intervention is necessary. | 2012 | 22965276 |
| chitin synthases with a myosin motor-like domain control the resistance of aspergillus fumigatus to echinocandins. | aspergillus fumigatus has two chitin synthases (csma and csmb) with a myosin motor-like domain (mmd) arranged in a head-to-head configuration. to understand the function of these chitin synthases, single and double csm mutant strains were constructed and analyzed. although there was a slight reduction in mycelial growth of the mutants, the total chitin synthase activity and the cell wall chitin content were similar in the mycelium of all of the mutants and the parental strain. in the conidia, ch ... | 2012 | 22964252 |
| expression and secretion of aspergillus fumigatus proteases are regulated in response to different protein substrates. | the ubiquitous filamentous fungus aspergillus fumigatus secretes a number of allergens with protease activity and has been linked to a variety of allergic conditions such as severe asthma with fungal sensitization (safs) and allergic bronchopulmonary aspergillosis (abpa). however, it is unclear which allergen proteases are being secreted during fungal invasion and whether the local biological environment regulates their expression. understanding the dynamic expression of allergen proteases durin ... | 2012 | 22954343 |
| clinical features of allergic bronchopulmonary aspergillosis in korea. | allergic bronchopulmonary aspergillosis (abpa) is a complex disease, triggered by a hypersensitivity reaction to the allergen aspergillus fumigatus. this disease occurs frequently in patients with cystic fibrosis and severe asthma in western countries, with a prevalence of 2%-15%. however, there have been only a few case reports in korea. we investigated the clinical and immunological features of patients with abpa. ten adult patients diagnosed with abpa, according to greenberger's criteria, wer ... | 2012 | 22950037 |
| identification of the urinary metabolites of glionitrin a in rats using ultra-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry. | glionitrin a (gn a) is a new diketopiperazine disulfide with an aromatic nitro group, which is isolated from the coculture of an aspergillus fumigatus fungal strain and a sphingomonas bacterial strain. after intravenous administration of gn a in rats, 13 urinary metabolites of gn a were identified using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectroscopy (uplc-qtop-ms) analysis in conjunction with data processing programs such as metabolynx™ and massfragnent™. red ... | 2012 | 22947415 |
| [severe contact lens-associated fungal keratitis]. | a 23-year-old man presented with severe contact lens-associated keratitis and descemetocele with pre-existing drug therapy. after 1 week of intensive antibiotic treatment aspergillus fumigatus was identified. despite adjusted antimycotic treatment a corneal perforation occurred. due to peripheral scleral infiltration the cornea was primarily closed with a double layer amniotic membrane in order to avoid a sclerokeratoplasty with a bad prognosis. after 2 weeks the peripheral corneal situation sta ... | 2012 | 22940808 |
| nucleotide oligomerization domain 2 contributes to the innate immune response in thce cells stimulated by aspergillus fumigatus conidia. | to investigate the expression of nucleotide oligomerization domain 2 (nod2) in the immortalized human corneal epithelial cell line (thce), and its role in the innate immune response triggered by inactive aspergillus fumigatus (af) conidia. | 2012 | 22937496 |
| the β-glucan receptor dectin-1 promotes lung immunopathology during fungal allergy via il-22. | sensitization to fungi, such as the mold aspergillus fumigatus, is increasingly becoming linked with asthma severity. we have previously shown that lung responses generated via the β-glucan receptor dectin-1 are required for lung defense during acute, invasive a. fumigatus infection. unexpectedly, in an allergic model of chronic lung exposure to live a. fumigatus conidia, β-glucan recognition via dectin-1 led to the induction of multiple proallergic (muc5ac, clca3, ccl17, ccl22, and il-33) and p ... | 2012 | 22933634 |
| aspergillus tracheobronchitis: report of 8 cases and review of the literature. | aspergillus tracheobronchitis (at) is an infrequent but severe form of invasive pulmonary aspergillosis in which the fungal infection is entirely or predominantly confined to the tracheobronchial tree. we reviewed 8 cases of at diagnosed in our tertiary care center during an 18-year period, as well as 148 cases previously reported in the english literature from 1985 to july 2011. the demographic, clinical, imaging, bronchoscopic, and outcome characteristics of every eligible patient were excerpt ... | 2012 | 22932790 |
| [effects of chronic aspergillus fumigatus exposure on the expression of mucin 5ac in the airways of asthmatic rats]. | to explore the effects of chronic aspergillus fumigatus (af) exposure on the expression of mucin 5ac (muc5ac) in the airways of asthmatic rats. | 2012 | 22931723 |
| structural insight into the mechanism of oxygen activation and substrate selectivity of flavin-dependent n-hydroxylating monooxygenases. | sida from the human pathogen aspergillus fumigatus catalyzes the generation of n(5)-hydroxyornithine in the biosynthesis of siderophores, a reaction essential for virulence. the crystal structures of sida in complex with ornithine and lysine reveal the geometry of the interactions among flavin, nadp(+), and the substrate amine group that underlie the hydroxylation reaction. the structural elucidation of the enzyme in complex with arginine provides insight into the role of electrostatics and hydr ... | 2012 | 22928747 |
| between-laboratory quality control of automated analysis of igg antibodies against aspergillus fumigatus. | measurement of igg antibodies against aspergillus fumigatus is an important criterion for the diagnosis of aspergilloma, allergic bronchopulmonary aspergillosis, and extrinsic allergic alveolitis. in the present study, we compared igg antibody analysis against a. fumigatus using 2 widely used automated immunochemistry systems. in a between-laboratory quality control program, good agreement was found between the results from laboratories that used the immunocap system; however, a laboratory that ... | 2012 | 22925654 |
| synthesis, crystal structure, characterisation, and antifungal activity of 3-thiophene aldehyde semicarbazone (3stch), 2,3-thiophene dicarboxaldehyde bis(semicarbazone) (2,3bstch2) and their nickel (ii) complexes. | the reaction of nickel (ii) chloride and bromide with 3-thiophene aldehyde semicarbazone (3stch) and 2,3-thiophene dicarboxaldehyde bis(semicarbazone) (2,3bstch(2)) leads to the formation of a series of new complexes: [nicl(2)(3stch)(2)], [nibr(2)(3stch)(2)], [nicl(2,3bstch(2))(h(2)o)]cl, and [nibr(2,3bstch(2))(h(2)o)]br respectively. the crystal structures of the two ligands 3stch, 2,3bstch(2) and of the complex [nibr(2,3bstch(2))(h(2)o)]br have been determined by x-ray diffraction methods. for ... | 2012 | 22922309 |
| a thermostable crude endoglucanase produced by aspergillus fumigatus in a novel solid state fermentation process using isolated free water. | aspergillus fumigatus was grown on chopped wheat straw in a solid state fermentation (ssf) process carried out in constant presence of isolated free water inside the fermentation chamber. the system allowed maintaining a constant vapor pressure inside the fermentor throughout the fermentation process. crude endoglucanase produced by a. fumigatus under such conditions was more thermostable than previously reported enzymes of the same fungal strain which were produced under different conditions an ... | 2012 | 22919467 |
| fad2-dgat2 genes coexpressed in endophytic aspergillus fumigatus derived from tung oilseeds. | recent efforts to genetically engineer plants that contain fatty acid desaturases to produce valuable fatty acids have made only modest progress. diacylglycerol acyltransferase 2 (dgat2), which catalyzes the final step in triacylglycerol (tag) assembly, might potentially regulate the biosynthesis of desired fatty acids in tags. to study the effects of tung tree (vernicia fordii) vfdgat2 in channeling the desired fatty acids into tag, vfdgat2 combined with the tung tree fatty acid desaturase-2 (v ... | 2012 | 22919314 |
| the antimicrobial, antioxidative, anti-inflammatory activity and cytotoxicity of different fractions of four south african bauhinia species used traditionally to treat diarrhoea. | many bauhinia species, including those indigenous to south africa, are used in traditional medicine across the world for treating ailments such as gastrointestinal tract (git) disorders, diabetes, infectious diseases and inflammation. | 2012 | 22917809 |
| zap1 regulates zinc homeostasis and modulates virulence in cryptococcus gattii. | zinc homeostasis is essential for fungal growth, as this metal is a critical structural component of several proteins, including transcription factors. the fungal pathogen cryptococcus gattii obtains zinc from the stringent zinc-limiting milieu of the host during the infection process. to characterize the zinc metabolism in c. gattii and its relationship to fungal virulence, the zinc finger protein zap1 was functionally characterized. the c. gattii zap1 gene is an ortholog of the master regulato ... | 2012 | 22916306 |
| comparative genome analysis between aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among aspergillus species. | aspergillus oryzae has been utilized for over 1000 years in japan for the production of various traditional foods, and a large number of a. oryzae strains have been isolated and/or selected for the effective fermentation of food ingredients. characteristics of genetic alterations among the strains used are of particular interest in studies of a. oryzae. here, we have sequenced the whole genome of an industrial fungal isolate, a. oryzae rib326, by using a next-generation sequencing system and com ... | 2012 | 22912434 |
| in vivo determination of mouse olfactory mucus cation concentrations in normal and inflammatory states. | olfaction is impaired in chronic rhinosinusitis (crs). the study has two aims: (1) to determine whether changes in cation concentration occur in the olfactory mucus of mice with crs, which may affect chemo-electrical transduction, (2) and to examine whether these alterations are physiologically significant in humans. | 2012 | 22911687 |
| the effect of aspergillus fumigatus infection on vitamin d receptor expression in cystic fibrosis. | aspergillus fumigatus (a. fumigatus) in cystic fibrosis (cf) is increasingly recognized. although allergic bronchopulmonary aspergillosis (abpa) leads to deterioration of pulmonary function, the effect of a. fumigatus colonization in the absence of abpa remains unclear. | 2012 | 22904183 |
| structural requirements for the activity of the mirb ferrisiderophore transporter of aspergillus fumigatus. | siderophores have been identified as virulence factors in the opportunistic fungal pathogen aspergillus fumigatus. the 14-pass transmembrane protein mirb is postulated to function as a siderophore transporter, responsible for uptake of the hydroxamate siderophore n,n',n″-triacetylfusarinine c (tafc). our aim was to identify amino acids of a. fumigatus mirb that are crucial for uptake of tafc. site-directed mutagenesis was used to create mirb mutants. expression of wild-type and mutant proteins i ... | 2012 | 22903978 |
| the aspergillus fumigatus protein glik protects against oxidative stress and is essential for gliotoxin biosynthesis. | the function of a number of genes in the gliotoxin biosynthetic cluster (gli) in aspergillus fumigatus remains unknown. here, we demonstrate that glik deletion from two strains of a. fumigatus completely abolished gliotoxin biosynthesis. furthermore, exogenous h(2)o(2) (1 mm), but not gliotoxin, significantly induced a. fumigatus glik expression (p = 0.0101). while both mutants exhibited significant sensitivity to both exogenous gliotoxin (p < 0.001) and h(2)o(2) (p < 0.01), unexpectedly, exogen ... | 2012 | 22903976 |
| in vitro-in vivo correlation of voriconazole resistance due to g448s mutation (cyp51a gene) in aspergillus fumigatus. | invasive pulmonary aspergillosis continues to be associated with a high mortality despite timely and appropriate therapy. although host immunity plays a major role in poor clinical response, antifungal drug resistance cannot be ignored. our studies were aimed 1) to study the mechanism of drug resistance in voriconazole strains of aspergillus fumigatus, 2) to establish a causal relationship between cyp51a mutation and voriconazole resistance (vrc-r), and 3) to determine whether vrc-r due to cyp51 ... | 2012 | 22897872 |
| synthesis and antifungal activity of derivatives of 2- and 3-benzofurancarboxylic acids. | we found that amiodarone has potent antifungal activity against a broad range of fungi, potentially defining a new class of antimycotics. investigations into its molecular mechanisms showed amiodarone mobilized intracellular ca2+, which is thought to be an important antifungal characteristic of its fungicidal activity. amiodarone is a synthetic drug based on the benzofuran ring system, which is contained in numerous compounds that are both synthetic and isolated from natural sources with antifun ... | 2012 | 22892340 |
| first reported case of azole-resistant aspergillus fumigatus due to the tr/l98h mutation in germany. | | 2012 | 22890769 |
| characterization of a chitosanase from aspergillus fumigatus atcc13073. | chitosanase ii was purified from the culture filtrate of aspergillus fumigatus atcc13073. the purified enzyme had a molecular mass of 23.5 kda. the n-terminal amino acid sequence of chitosanase ii was identical to those of other aspergillus chitosanases belonging to glycoside hydrolase family 75. the optimum ph and temperature were ph 6.0 and 40 °c. chitosanase ii hydrolyzed 70% deacetylated chitosan faster than fully deacetylated chitosan. analysis of the degradation products generated from par ... | 2012 | 22878198 |
| interkingdom metabolic transformations captured by microbial imaging mass spectrometry. | in polymicrobial infections, microbes can interact with both the host immune system and one another through direct contact or the secretion of metabolites, affecting disease progression and treatment options. the thick mucus in the lungs of patients with cystic fibrosis is highly susceptible to polymicrobial infections by opportunistic pathogens, including the bacterium pseudomonas aeruginosa and the fungus aspergillus fumigatus. unravelling the hidden molecular interactions within such polymicr ... | 2012 | 22869730 |
| robust polyfunctional t-helper 1 responses to multiple fungal antigens from a cell population generated using an environmental strain of aspergillus fumigatus. | aspergillus fumigatus infections are the leading cause of invasive fungal infection-related deaths in stem cell transplant patients, and may be amenable to correction with adoptive immunotherapy providing t lymphocytes specific for a. fumigatus. however, a clinically usable source of antigen and a reliable procedure for the generation of large numbers of aspergillus-specific t lymphocytes to clinical-grade standards is not available. | 2012 | 22866666 |
| multiplexed activity-based protein profiling of the human pathogen aspergillus fumigatus reveals large functional changes upon exposure to human serum. | environmental adaptability is critical for survival of the fungal human pathogen aspergillus fumigatus in the immunocompromised host lung. we hypothesized that exposure of the fungal pathogen to human serum would lead to significant alterations to the organism's physiology, including metabolic activity and stress response. shifts in functional pathway and corresponding enzyme reactivity of a. fumigatus upon exposure to the human host may represent much needed prognostic indicators of fungal infe ... | 2012 | 22865858 |
| aspergillus tanneri sp. nov., a new pathogen that causes invasive disease refractory to antifungal therapy. | the most common cause of invasive aspergillosis (ia) in patients with chronic granulomatous disease (cgd) is aspergillus fumigatus followed by a. nidulans; other aspergilli rarely cause the disease. here we review two clinical cases of fatal ia in cgd patients and describe a new etiologic agent of ia refractory to antifungal therapy. unlike typical ia caused by a. fumigatus, the disease caused by the new species was chronic and spread from the lung to multiple adjacent organs. mycological charac ... | 2012 | 22855513 |
| regulation of dendritic cell functions against harmful respiratory pathogens by a cysteinyl leukotrienes receptor antagonist. | cysteinyl leukotriene receptor antagonist (ltra) is a widely used medicine for asthma. cysteinyl leukotrienes (cyslts) are involved in the regulation of dendritic cell (dc) function. however, the effects of ltra on dc-related antimicrobial immunity against harmful respiratory pathogens remain unknown. the purpose of this study was to examine the effects of ltra administered in vivo on dc function against representative respiratory pathogens in vitro. pulmonary dcs were isolated from four groups ... | 2012 | 22852127 |
| analysis of promoter function in aspergillus fumigatus. | the filamentous fungus aspergillus fumigatus is an important opportunistic pathogen that can cause high mortality levels in susceptible patient populations. the increasing dependence on antifungal drugs to control a. fumigatus has led to the inevitable acquisition of drug-resistant forms of this pathogen. in other fungal pathogens, drug resistance is often associated with an increase in transcription of genes such as atp-binding cassette (abc) transporters that directly lead to tolerance to comm ... | 2012 | 22843562 |
| interleukin-12 and interleukin-2 alone or in combination against the infection in invasive pulmonary aspergillosis mouse model. | aspergillus fumigatus is an intracellular opportunistic fungus causing invasive pulmonary mycosis, characterised by hyphal invasion and destruction of pulmonary tissue. th1 cytokines could enhance fungicidal activity. the effects from the combination of interleukin-12 (il-12) and il-2 are rarely known in invasive pulmonary aspergillosis infection. to assess the cleaning of a. fumigatus infection in the pulmonary tissues by il-12 and il-2, interferon-γ (ifn-γ) was detected in the sera using elisa ... | 2013 | 22834482 |
| assessment of aspergillus sensitization or persistent carriage as a factor in lung function impairment in cystic fibrosis patients. | cystic fibrosis (cf) patients presenting with persistent carriage of, or sensitization to, aspergillus fumigatus are often treated with antifungal therapies because the presence of the fungus is commonly thought to impede lung function, even in the absence of allergic bronchopulmonary aspergillosis (abpa). the aim of this study was to assess aspergillus-related status modulating the forced expiratory volume in 1 s (fev₁) of cf patients. | 2012 | 22831545 |
| combination of voriconazole and anidulafungin for treatment of triazole-resistant aspergillus fumigatus in an in vitro model of invasive pulmonary aspergillosis. | voriconazole is a first-line agent for the treatment of invasive pulmonary aspergillosis. isolates with elevated voriconazole mics are increasingly being seen, and the optimal treatment regimen is not defined. we investigated whether the combination of voriconazole with anidulafungin may be beneficial for the treatment of a. fumigatus strains with elevated voriconazole mics. we used an in vitro model of the human alveolus to define the exposure-response relationships for a wild-type strain (vori ... | 2012 | 22825124 |
| serum igg responses against aspergillus proteins before hematopoietic stem cell transplantation or chemotherapy identify patients who develop invasive aspergillosis. | the ability to identify patients at particularly low risk for invasive aspergillosis (ia) would facilitate more efficient targeting of antifungal prophylaxis. we measured baseline serum immunoglobulin responses against 6 purified recombinant aspergillus fumigatus proteins before hematopoietic stem cell transplantation (hsct) or chemotherapy in 73 subjects, including 19 patients who subsequently developed proven or probable ia and 54 uninfected controls. we also assessed responses at the time of ... | 2012 | 22824184 |
| heat shock protein 90 is required for conidiation and cell wall integrity in aspergillus fumigatus. | heat shock protein 90 (hsp90) is a eukaryotic molecular chaperone. its involvement in the resistance of candida albicans to azole and echinocandin antifungals is well established. however, little is known about hsp90's function in the filamentous fungal pathogen aspergillus fumigatus. we investigated the role of hsp90 in a. fumigatus by genetic repression and examined its cellular localization under various stress conditions. failure to generate a deletion strain of hsp90 suggested that it is es ... | 2012 | 22822234 |
| incidence and risk factors for infection in oral cancer patients undergoing different treatments protocols. | over the past decade, advances in cancer treatments have been counterbalanced by a rising number of immunosuppressed patients with a multitude of new risk factors for infection. hence, the aim of this study was to determine risk factors, infectious pathogens in blood and oral cavity of oral cancer patients undergoing different treatment procedures. | 2012 | 22817766 |
| chapter 18: allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) occurs in patients with asthma or cystic fibrosis resulting in pulmonary infiltrates, tenacious mucus plugs that harbor hyphae of aspergillus fumigatus, elevations of total serum ige concentration and peripheral blood, and sputum eosinophilia. bronchiectasis is an irreversible complication of abpa. the key to early diagnosis is considering abpa in anyone with asthma or cystic fibrosis and a positive skin test to aspergillus and /or recurrent infiltr ... | 2016 | 22794691 |
| comparative analysis of calcineurin signaling between candida dubliniensis and candida albicans. | candida dubliniensis, an emerging fungal pathogen, is the closest known species to the established pathogenic species candida albicans. despite the fact that these two species share > 80% genome sequence identity, they exhibit distinct properties such as less hyphal growth, reduced pathogenicity and increased sensitivity to sodium stress and elevated temperatures in c. dubliniensis compared with c. albicans. it is, however, largely unknown whether signaling pathways are conserved in the two cand ... | 2012 | 22808313 |
| a miqe-compliant real-time pcr assay for aspergillus detection. | the polymerase chain reaction (pcr) is widely used as a diagnostic tool in clinical laboratories and is particularly effective for detecting and identifying infectious agents for which routine culture and microscopy methods are inadequate. invasive fungal disease (ifd) is a major cause of morbidity and mortality in immunosuppressed patients, and optimal diagnostic criteria are contentious. although pcr-based methods have long been used for the diagnosis of invasive aspergillosis (ia), variable p ... | 2012 | 22808087 |
| fungicidal activity of lysozyme is inhibited in vitro by commercial sinus irrigation solutions. | lysozyme is an innate immune peptide with bactericidal and fungicidal activity (fa). despite increased expression of lysozyme protein in chronic rhinosinusitis (crs) sinus mucosa, crs patients experience repeated bacterial and/or fungal infections. commercial sinus irrigation solutions are often used to provide symptomatic relief. however, one of the mechanisms of action of lysozyme involves ionic interactions with the microbial cell wall, which may be inhibited by ionic solutions such as commer ... | 2012 | 22801018 |
| antidiabetic, anti-oxidant and antimicrobial activities of fadogia ancylantha extracts from malawi. | communities in chilumba, malawi use herbal tea prepared from fadogia ancylantha schweinf (rubiaceae) leaves for the management of diabetes, hypertension and alleviation of symptoms of gastrointestinal disorders and pneumonia. the objective of the study was to evaluate the in vitro antidiabetic, anti-oxidant and antimicrobial activities of the crude extracts of the leaves prepared by using three different extraction methods. | 2012 | 22800680 |
| fungal aerosol in the process of poultry breeding--quantitative and qualitative analysis. | the aim of this study was to assess fungal air contamination in the processes associated with poultry breeding depending on the season. the evaluation was based on the determined concentrations of fungi and qualitative identification of isolated microorganisms. | 2012 | 22774459 |
| interactions of liposome carriers with infectious fungal hyphae reveals the role of β-glucans. | relatively little is known about how liposomal formulations modulate drug delivery to fungal pathogens. we compared patterns of hyphal cell wall binding for empty rhodmine-labeled liposomes and the clinically available amphotericin b-containing liposomal formulation (ambisome) in aspergillus fumigatus and candida albicans. following 0.5 h of coincubation with a. fumigatus , empty liposomes concentrated primarily in fungal septae along at the surface of the cell wall, suggesting that liposome upt ... | 2012 | 22770505 |