| listeria monocytogenes internalins are highly diverse and evolved by recombination and positive selection. | to probe the evolution of internalins with confirmed or suspected roles in listeria monocytogenes virulence we sequenced the full inlb, inlc2, inlc, inld, inle, inlf, inlg, and inlh orfs from 40 l. monocytogenes isolated from human (n=10) and animal (n=10) clinical cases, foods (n=10), and the natural environment (n=10). inlb and inle were present in all isolates, representing 26 and 20 alleles, respectively. inlc was found in all lineage i and ii isolates and represented 21 alleles. inlc2 and i ... | 2006 | 16473049 |
| primary and opportunistic pathogens associated with meningitis in adults in bangui, central african republic, in relation to human immunodeficiency virus serostatus. | to determine the causative organisms and characteristics of patients presenting with meningitis in bangui in order to provide guidance to physicians for case management. | 2006 | 16473538 |
| impaired response to listeria in h2-m3-deficient mice reveals a nonredundant role of mhc class ib-specific t cells in host defense. | the major histocompatibility complex (mhc) class ib molecule h2-m3 primes the rapid expansion of cd8+ t cells by presenting n-formylated bacterial peptides. however, the significance of h2-m3-restricted t cells in host defense against bacteria is unclear. we generated h2-m3-deficient mice to investigate the role of h2-m3 in immunity against listeria monocytogenes (lm), a model intracellular bacterial pathogen. h2-m3-deficient mice are impaired in early bacterial clearance during primary infectio ... | 2006 | 16476767 |
| monoclonal antibodies binding to the cell surface of listeria monocytogenes serotype 4b. | serotype 4b strains of the food-borne pathogen listeria monocytogenes are responsible for a large portion of sporadic listeric infections and all major food-borne listeriosis outbreaks in humans. hybridomas were produced from three fusions with lymphocytes of nd4 mice immunized either with the insoluble antigens of l. monocytogenes serotype 4b or with formalin-killed bacterial cells and screened for monoclonal antibodies (mabs) reactive to l. monocytogenes serotype 4b. a set of 35 mabs was ident ... | 2006 | 16476793 |
| occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in spain. | to survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish. | 2006 | 16478492 |
| evidence of an antilisterial factor induced by wounding of iceberg lettuce tissues. | to examine the influence of wound-associated reactions in cut iceberg lettuce (lactuca sativa l.) tissues on the fate of listeria monocytogenes. | 2006 | 16478519 |
| [listeria monocytogenes meningitis complicated after operation for thoracic aortic aneurysm]. | the patient was a 71-year-old male. he was admitted as an emergency to our hospital because of impending rupture of thoracic aortic aneurysm with anterior chest pain and hoarseness. the aneurysm was saccular type and located in the lesser curvature of aortic arch. emergency operation was performed. entry of the aneurysm was excluded by including artificial graft on the deep hypothermic circulatory arrest. postoperative course was uneventful, but sudden depression of consciousness level and pyrex ... | 2006 | 16482907 |
| characterization of the bifunctional glycosyltransferase/acyltransferase penicillin-binding protein 4 of listeria monocytogenes. | multimodular penicillin-binding proteins (pbps) are essential enzymes responsible for bacterial cell wall peptidoglycan (pg) assembly. their glycosyltransferase activity catalyzes glycan chain elongation from lipid ii substrate (undecaprenyl-pyrophosphoryl-n-acetylglucosamine-n-acetylmuramic acid-pentapeptide), and their transpeptidase activity catalyzes cross-linking between peptides carried by two adjacent glycan chains. listeria monocytogenes is a food-borne pathogen which exerts its virulenc ... | 2006 | 16484198 |
| mammalian peptidoglycan recognition protein tagl inhibits listeria monocytogenes invasion into epithelial cells. | peptidoglycan recognition proteins are a family of evolutionary conserved proteins that play a basic role in the innate immunity of insects, but their role in the immunity of mammals remains unclear. to elucidate its functions, a mouse member of the peptidoglycan recognition proteins family, tagl, was stably expressed in colon adenocarcinoma ht29 cells, and its effect on the invasion and intracellular growth of the enteroinvasive pathogenic bacterium listeria monocytogenes was assessed. the expr ... | 2006 | 16487310 |
| a combination of diagnostic tools for rapid screening of ovine listeriosis. | a combined serological and pcr method for the detection of listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. seventy-eight milk samples and 157 serum samples were analysed using a l. monocytogenes pcr detection kit and an anti-listeriolysin o igg immunoassay kit. the combined use of these commercial kits allowed a rapid and effective detection of l. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after ... | 2006 | 16487553 |
| effect of environmental parameters (temperature, ph and a(w)) on the individual cell lag phase and generation time of listeria monocytogenes. | the effect of the individual environmental factors temperature (2-30 degrees c), ph (4.4-7.4) and a(w) (0.947-0.995) as well as the combinations of these factors on the individual cell lag phase and the generation time of listeria monocytogenes was investigated. individual cells were isolated using a serial dilution protocol in microtiter plates, and subsequent growth was investigated by optical density (od) measurements at 600 nm. about 100 replicates were made for each set of environmental con ... | 2006 | 16488043 |
| adsorption of a biosurfactant on surfaces to enhance the disinfection of surfaces contaminated with listeria monocytogenes. | the effects of sodium hypochlorite (naocl) and peracetic acid/hydrogen peroxide (pah) on the inactivation of adherent listeria monocytogenes lo28 cells were examined. the surfaces tested were stainless steel and polytetrafluoroethylene (ptfe) conditioned or not with an anionic biosurfactant produced by pseudomonas fluorescens. dilution-neutralization methods were used to assess the effectiveness of sanitizer solutions on planktonic and adherent cells. tests were performed on l. monocytogenes cul ... | 2006 | 16488496 |
| a role for cortactin in listeria monocytogenes invasion of nih 3t3 cells, but not in its intracellular motility. | cortactin is an f-actin binding protein that binds to the arp2/3 complex, stimulates its actin nucleation activity, and inhibits actin filament debranching. using rna interference directed against cortactin, we explored the importance of cortactin for several processes involving dynamic actin assembly. silencing cortactin expression was efficiently achieved in hela and nih 3t3 cells, with less than 5% of cortactin expression in sirna-treated cells. surprisingly, endocytosis in hela and nih 3t3 c ... | 2006 | 16489553 |
| antimicrobial activity of lysostaphin and a listeria monocytogenes bacteriophage endolysin produced and secreted by lactic acid bacteria. | the expression and secretion signals of the sep protein from lactobacillus fermentum br11 were used to direct export of two peptidoglycan hydrolases by lb. fermentum br11, lactobacillus rhamnosus gg, lactobacillus plantarum atcc 14917 and lactococcus lactis mg1363. the production levels, hydrolytic and bacteriocidal activities of the listeria monocytogenes bacteriophage n-acetylmuramoyl-l-alanine amidase endolysin ply511 and the glycylglycine endopeptidase lysostaphin were examined. buffering of ... | 2007 | 16490333 |
| consumer phase risk assessment for listeria monocytogenes in deli meats. | the foodborne disease risk associated with the pathogen listeria monocytogenes has been the subject of recent efforts in quantitative microbial risk assessment. building upon one of these efforts undertaken jointly by the u.s. food and drug administration and the u.s. department of agriculture (usda), the purpose of this work was to expand on the consumer phase of the risk assessment to focus on handling practices in the home. one-dimensional monte carlo simulation was used to model variability ... | 2006 | 16492183 |
| risk assessment of listeria monocytogenes: impact of individual cell variability on the exposure assessment step. | recently, the lag phase research in predictive microbiology is focusing more on the individual cell variability, especially for pathogenic microorganisms that typically occur in very low contamination levels, like listeria monocytogenes. in this study, the effect of this individual cell lag phase variability was introduced in an exposure assessment study for l. monocytogenes in a liver pâté. a basic framework was designed to estimate the contamination level of pâté at the time of consumption, ta ... | 2006 | 16492184 |
| the onset of cd8+-t-cell contraction is influenced by the peak of listeria monocytogenes infection and antigen display. | the cd8+-t-cell response to infection with listeria monocytogenes consists of expansion, contraction, and memory phases. the transition between expansion and contraction is reported to occur on different days postinfection with virulent (8 to 9 days) and attenuated (deltaacta) (7 days) l. monocytogenes strains. we hypothesized that differences in the infectious courses, and therefore antigen (ag) display, determine the precise time of the expansion/contraction transition in response to these inf ... | 2006 | 16495523 |
| induced biliary excretion of listeria monocytogenes. | listeria monocytogenes is a ubiquitous gram-positive bacterium that can cause systemic and often life-threatening disease in immunocompromised hosts. this organism is largely an intracellular pathogen; however, we have determined that it can also grow extracellularly in animals, in the lumen of the gallbladder. the significance of growth in the gallbladder with respect to the pathogenesis and spread of listeriosis depends on the ability of the bacterium to leave this organ and be disseminated to ... | 2006 | 16495556 |
| characteristics of listeria monocytogenes isolated from imported meat in japan. | the genomic structure of the iap region in listeria monocytogenes (serovar 4b), isolated from chicken imported into japan, was compared with those from japanese strains. the isolate was similar to the japanese strains in a comparatively new, rare group. such strains might be imported from foreign countries. | 2006 | 16495636 |
| [neonatal maculopapular exanthema revealing septicemia due to klebsiella pneumoniae via maternofetal infection]. | neonatal exanthema of bacterial origin is caused very rarely by gram-negative bacilli. we report a case of neonatal maculopapular exanthema evocative of klebsiella pneumoniae septicemia resulting from maternofetal infection. | 2006 | 16495848 |
| pathogen reduction in unpasteurized apple cider: adding cranberry juice to enhance the lethality of warm hold and freeze-thaw steps. | u.s. food and drug administration (fda) regulations require processors of apple cider sold wholesale to use processing steps that ensure a 5-log reduction in numbers of the pertinent pathogen, generally considered to be escherichia coli o157:h7. current widely used validated pathogen-reduction steps are thermal pasteurization and uv light treatment. these techniques may be unaffordable or undesirable for some processors. this study investigated the cran-cider process, which is the addition of cr ... | 2006 | 16496568 |
| attributing risk to listeria monocytogenes subgroups: dose response in relation to genetic lineages. | the objective of this study was to evaluate the hypothesis that the dose-response relationship for listeria monocytogenes in humans varies with genotypic lineage or subtype. the linkages between molecular subtyping data and enumeration data for l. monocytogenes subtypes in foods consumed by the at-risk population were examined to test this hypothesis. we applied a conditional probability model to conduct a subtype-specific dose-response analysis, with the focus on invasive listeriosis. l. monocy ... | 2006 | 16496574 |
| a solid agar overlay method for recovery of heat-injured listeria monocytogenes. | a solid agar overlay method was developed for recovery of heat-injured listeria monocytogenes. presolidified nonselective tryptic soy agar with 0.6% yeast extract (tsaye, 2% agar) was overlaid on top of solidified modified oxford agar (mox). heat injury of l. monocytogenes was conducted at 58 degrees c for 6 min in a jacketed flask filled with tryptic soy broth. both noninjured and heat-treated l. monocytogenes cells were plated onto tsaye, mox, and tsaye-mox plates. no significant differences ( ... | 2006 | 16496588 |
| acidified sodium chlorite treatment for inhibition of listeria monocytogenes growth on the surface of cooked roast beef. | the effects of acidified sodium chlorite (asc) against listeria monocytogenes on the surface of cooked roast beef were investigated. l. monocytogenes, strain v7, serotype 1/2a, was inoculated at numbers of 6.0 log cfu/g onto 5-g cubes of cooked regular or spicy roast beef. the samples were allowed to air dry for 1 h. the cooked roast beef samples were dipped into asc or sprayed with asc solutions of 250, 500, 750, or 1,000 ppm, then placed in bags with or without a vacuum and refrigerated at 4 d ... | 2006 | 16496589 |
| prevalence and genetic characterization of listeria monocytogenes in retail broiler meat in estonia. | the prevalence and genetic diversity of listeria monocytogenes in raw broiler legs at the retail level in estonia were studied. a total of 240 raw broiler legs (120 from estonia and 120 of foreign origin, which had been imported to estonia from denmark, finland, hungary, sweden, and the united states) from 12 retail stores in the two largest cities in estonia (tallinn and tartu) were investigated from january to december 2002. of these, 70% were positive for l. monocytogenes. the prevalence of l ... | 2006 | 16496590 |
| incidence of enterohemorrhagic escherichia coli, escherichia coli o157, salmonella, and listeria monocytogenes in retail fresh ground beef, sprouts, and mushrooms. | the objective of this study was to determine the prevalence of enterohemorrhagic escherichia coli (ehec), e. coli o157, salmonella, and listeria monocytogenes in retail food samples from seattle, wash. a total of 2,050 samples of ground beef (1,750 samples), mushrooms (100 samples), and sprouts (200 samples) were collected over a 12-month period and analyzed for the presence of these pathogens. pcr assays, followed by culture confirmation were used to determine the presence or absence of each or ... | 2006 | 16496591 |
| radiation processing to ensure safety of minimally processed carrot (daucus carota) and cucumber (cucumis sativus): optimization of dose for the elimination of salmonella typhimurium and listeria monocytogenes. | minimally processed vegetables are in demand, because they offer convenience to consumers. however, these products are often unsafe because of possible contamination with pathogens, such as salmonella, escherichia coli o157:h7, and shigella species. therefore, this study was carried out to optimize the radiation dose necessary to ensure the safety of precut carrot and cucumber. decimal reduction doses (d-values) of salmonella typhimurium mtcc 98 were ca. 0.164 kgy in carrot samples and 0.178 kgy ... | 2006 | 16496592 |
| surface pasteurization of vacuum-sealed precooked ready-to-eat meat products. | pathogens may contaminate ready-to-eat meat products after cooking but before packaging. listeria monocytogenes is a formidable contaminant in the food processing environment and is relatively heat resistant compared with other non-spore-forming pathogens. as a consequence, this microorganism is commonly chosen for evaluation in postpackage pasteurization studies. the aim of this study was to review information on the thermal surface pasteurization of vacuum-sealed precooked ready-to-eat meat pr ... | 2006 | 16496595 |
| [mutagenesis on biofilm formation of listeria monocytogenes by tn917 transposon insertion]. | surface-attached populations of bacteria comprising either single or multiple species were referred to as biofilm. the bacteria in the biofilm had more resistant ability against antibiotics and disinfectors, which could cause the constituent clinical affection or food contamination. in order to isolate and characterize the genes involved in biofilm formation of listeria monocytogenes, the plasmid ptv1-ok containing the transposon tn917 was transformed into the protoplasm of listeria monocytogene ... | 2005 | 16496710 |
| adhesion characteristics of listeria adhesion protein (lap)-expressing escherichia coli to caco-2 cells and of recombinant lap to eukaryotic receptor hsp60 as examined in a surface plasmon resonance sensor. | listeria adhesion protein (lap) is an important adhesion factor in listeria monocytogenes and interacts with its cognate receptor, mammalian heat shock protein 60 (hsp60). the genetic identity of lap was determined to be alcohol acetaldehyde dehydrogenase (aad). a recombinant escherichia coli strain expressing aad confirmed the involvement of aad in adhesion to caco-2 cells. binding kinetics (ka) of recombinant lap (rlap) to hsp60 was examined in a surface plasmon resonance sensor and was determ ... | 2006 | 16499624 |
| bayesian synthesis of a pathogen growth model: listeria monocytogenes under competition. | the bayesian synthesis method is applied to data from two studies of listeria monocytogenes grown in broth monocultures to draw inferences about the joint distribution of two baranyi growth model parameters-lag time and maximum specific growth rate. the resultant joint distribution is then combined with prior distributions for the initial and maximum pathogen density parameters under competitive growth conditions. finally, the pathogen growth model is updated using the sampling/importance resamp ... | 2006 | 16499986 |
| cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, lactobacillus brevis opk-3. | lactobacillus brevis opk-3, having 84.292 mg/l/h of gamma-aminobutyric acid (gaba) productivity, was isolated from kimchi, a traditional fermented food in korea. a core fragment of glutamate decarboxylase (gad) dna was isolated from the l. brevis opk-3, using primers based on two highly conserved regions of gad. a full-length gad (lbgad) clone was subsequently isolated through rapid amplification of cdna ends (race) pcr. nucleotide sequence analysis revealed that the open reading frame (orf) con ... | 2007 | 16500100 |
| acute bacterial meningitis in qatar. | to study the changes in the epidemiology, clinical and bacteriological profiles of bacterial meningitis in the era of the haemophilus influenzae type b (hib)vaccine and pneumococcus resistance. | 2006 | 16501676 |
| [serovars of listeria monocytogenes and related species isolated from human clinical specimens]. | using phenotype techniques, characterization was realized of species and serovars of 255 strains of listeria isolated from human material: 220-86.3% were from patients with possible invasive disease, while 35-13.7% were from colonized healthy individuals. the strains were collected in several regions of brazil from 1969-2000. in individuals aged 0-10 or 41-60 years old listeria monocytogenes was isolated more often in cerebral spinal fluid than in blood cultures, including samples from renal tra ... | 2006 | 16501763 |
| microbiological quality of randomly selected ready-to-eat foods sampled between 2003 and 2005 in wales, uk. | since 1995, the publicly funded ready-to-eat food sampling and examination activities in wales have been coordinated and structured, using a novel approach for the identification of samples and premises. the latest set of data from this surveillance system reports the results from 3391 ready-to-eat foods sampled between november 2003 and march 2005. during this seventeen-month period all samples were examined for aerobic colony count, escherichia coli, listeria spp., bacillus cereus, salmonella, ... | 2006 | 16503065 |
| lentinan has a stimulatory effect on innate and adaptive immunity against murine listeria monocytogenes infection. | lentinan, a (1-3)-beta glucan from lentinus edodes, is licensed as an immunostimulatory drug. we tested the effect of lentinan in the well-established model system of the murine listeria monocytogenes infection. pre-treatment of bone marrow macrophages and dendritic cells with lentinan resulted in increased production of tnf-alpha and il-12 after l. monocytogenes infection in vitro. after lentinan treatment bone marrow macrophages showed increased no-production and enhanced cytotoxic activity ag ... | 2006 | 16504933 |
| [zoonotic importance of selected species of gram-positive bacteria.] | zoonoses continue to play a signicant role in both human and veterinary medicine. their eradication is difficult and some authors are convinced of their growing importance. among the pathogens of infectious diseases transmissible from animals to man are several gram-positive bacteria. the authors focus on three agents that present a risk in three different areas-alimentary infections (listeria monocytogenes), professional infections (streptococcus suis) and infections of breeders of so-called pe ... | 2006 | 16508905 |
| infection with listeria monocytogenes as a probe for placental immunological function. | this chapter will describe the use of infection with listeria monocytogenes, a gram-positive intracellular bacterium, to study immunological responses in the placenta. this bacterium is chosen because it has a predilection for replication in the placenta. as such, it is a significant pathogen for pregnant women, being a major cause of fetal mortality and morbidity if appropriate public health precautions are not observed. furthermore, this bacterium has been a major tool for studying innate immu ... | 2006 | 16512000 |
| improved dna probe detection of listeria monocytogenes in enrichment culture after physical-chemical fractionation. | bacterial detection in foods by nucleic acid probes is limited by microflora competition during selective enrichment. probe target concentration by extraction and fractionation of enrichments may diminish this limitation. the 1-h accuprobe chemiluminescent culture identification test for listeria monocytogenes was used as a model. its high detection threshold provides a stringent challenge for evaluating enrichment work-up protocols. detection of l. monocytogenes, at 1-4 colony-forming units/g f ... | 2006 | 16512244 |
| probiotic lactobacillus casei activates innate immunity via nf-kappab and p38 map kinase signaling pathways. | probiotic bacteria are microorganisms that benefit the host through improvement of the balance of intestinal microflora and possibly by augmentation of host defense systems. we examined the mechanisms for the up-regulation of innate immune responses by a probiotic lactobacillus casei atcc27139, in vivo. using mouse models of systemic listeria monocytogenes infection and metha fibrosarcoma tumorigenesis in combination with balb/c and scid mice, we found that parenteral administration of l. casei ... | 2006 | 16513392 |
| colon cancer cells: pro-invasive signalling. | colon cancer results from erroneous renewal of the enteric epithelium. mutations in stem cells, or their proliferative progenitors, cause accumulation of cells that invade into the stroma and continue to divide rather than migrating on top of the basement membrane prior to entering into apoptosis. many of these changes in invasive activity appear to be related to the invasion-suppressor role of e-cadherin. we have also investigated listeria monocytogenes and other enteric bacteria, since these b ... | 2006 | 16513408 |
| genetic and phenotypic characterization of listeria monocytogenes lineage iii. | listeria monocytogenes has been previously grouped into three evolutionary groups, termed lineages i, ii and iii. while lineages i and ii are commonly isolated from various sources, lineage iii isolates are rare and have several atypical and unique phenotypic characteristics. relative to their prevalence in other sources, lineage iii strains are overrepresented among isolates from food-production animals, and underrepresented among isolates from human clinical cases and foods. this work describe ... | 2006 | 16514149 |
| [infliximab therapy in crohn's disease complicated by listeria monocytogenes meningoencephalitis]. | | 2006 | 16514404 |
| comparison of two chromogenic media for the detection of listeria monocytogenes with the plating media recommended by en/din 11290-1. | the efficacy of two selective chromogenic culture media, agar listeria ottaviani and agosti (aloa) and rapid' l. mono for the detection of listeria monocytogenes in food, was compared with that of an official culture method according to the en/din 11290-01 and -02 protocols [corresponding to the section 35 lmbg (german food act) method]. a total of 310 pre-packed ready-to-eat food samples (100 of graved and cold smoked salmon, 130 of different raw and cooked sausages and 80 of delicatessen and m ... | 2006 | 16515816 |
| heterologous expression of betl, a betaine uptake system, enhances the stress tolerance of lactobacillus salivarius ucc118. | given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. the nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system betl in the probiotic strain lactobacillus salivarius ucc118. following nisin induction, strains expressing betl exhibited a significant increase in resistance to several stresses, ... | 2006 | 16517668 |
| tolerance of listeria monocytogenes to cell envelope-acting antimicrobial agents is dependent on sigb. | mutation of sigb impairs the ability of listeria monocytogenes to grow in sublethal levels, and to survive in lethal concentrations, of the bacteriocins nisin and lacticin 3147 and the antibiotics ampicillin and penicillin g. sigb may therefore represent an attractive target for the development of new control and treatment strategies for this important pathogen. | 2006 | 16517678 |
| cutting edge: the direct action of type i ifn on cd4 t cells is critical for sustaining clonal expansion in response to a viral but not a bacterial infection. | the action of type i ifn (ifn-i) on apcs is well studied, but their direct effect on cd4 t cells is unclear. to address this, we transferred ifn-i receptor-deficient (ifn-ir(0)) and -sufficient (wild-type, wt) tcr-transgenic cd4 t cells into wt mice and analyzed their response to immunization. in response to lymphocytic choriomeningitis virus immunization, wt cd4 t cells expanded approximately 100-fold, whereas ifn-ir(0) cd4 t cells expanded <10-fold. however, both wt and ifn-ir(0) cd4 t cells e ... | 2006 | 16517698 |
| neonatal septicemia in high risk babies in south-eastern nigeria. | septicemia is one of the major causes of morbidity and mortality in the neonatal period and it often has a rapid and fulminant course. | 2006 | 16519624 |
| veterinary and medical aspects of abortion in danish sheep. | the danish sheep population totals around 144,000 animals, but little is known of the causes and prevalance of diseases. this study focuses on the causes of abortion in danish sheep. during one breeding season, aborted foetuses and stillbirths with signs of intrauterine death or malformation were submitted for laboratory examination from a population of 3,758 breeding ewes. samples from 24 incidents of abortion and 21 ewes delivering malformed lambs or lambs with ante partum decomposition were s ... | 2006 | 16519752 |
| invasiveness is a variable and heterogeneous phenotype in listeria monocytogenes serotype strains. | the ability of listeria monocytogenes to breach mucosal and endothelial barriers of the host during infection is a hallmark property mediated by the internalins (inl) a and b. we examined the invasive property of several l. monocytogenes strains representing 13 serotypes. we found that invasiveness is a heterogeneous phenotype amongst l. monocytogenes serotype strains. despite this, many of the poorly invasive and non-invasive strains of l. monocytogenes express internalins at levels comparable ... | 2006 | 16527541 |
| the ferritin-like protein frm is a target for the humoral immune response to listeria monocytogenes and is required for efficient bacterial survival. | the identity and role of listerial antigens recognized by antibodies following listerial infection is largely unknown. we identified the listerial ferritin-like protein frm as a target of the humoral response following infection of mice with pathogenic listeria monocytogenes. specific antibodies to frm are detected in antisera of mice infected with a pathogenic strain but not in antisera of mice infected with a non-pathogenic l. innocua strain. antibodies raised to purified listerial ferritin al ... | 2006 | 16528570 |
| [agglutination as screening test in routine diagnostic of listeriosis]. | the persons with weakened immunity, newborn infants and pregnant women are very much sensitive on infection with bacteria listeria monocytogenes. listeria can readily invade the placenta and precipitate premature labor and fetal death. to confirm diagnosis is very difficult because listeriosis is disease very similar to the others acute diseases. serum threshold values above 1: 320 confirm think about listeriosis when the relevant clinical symptoms are present. of greater diagnostic relevance is ... | 2006 | 16528925 |
| genotypic and phenotypic heterogeneity in enterococcus isolates from batzos, a raw goat milk cheese. | this study investigated the genotypic and phenotypic diversity in 34 isolates of enterococci obtained during ripening of batzos cheese from raw goat milk and characterized phenotypically as enterococcus durans. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. species recognition by means of rapd-pcr was in agreement with the phenotypic identification for 29 strains. one strain was characterized as lactococcus lactis subsp. ... | 2006 | 16529834 |
| [functional activity of mouse peritoneal macrophages contaminated with gram-positive bacteria]. | the analysis of the functional and enzymatic activity of mouse peritoneal macrophages contaminated with staphylococcus aureus and listeria monocytogenes virulent strains is presented. the low bactericidal and digestive activity of these cells with respect to the above-mentioned microorganisms was determined. in this study a decrease in the activity of plasmatic membrane enzymes (5'-nucleotidase and atp-ase) of macrophages contaminated with s. aureus and l. monocytogenes was observed, which was i ... | 2006 | 16532641 |
| role of lung surfactant in phagocytic clearance of apoptotic cells by macrophages in rats. | two of the common features of inflammatory lung diseases are the increased production of pulmonary surfactant and the induction of lung cell apoptosis. however, the relationship between these two events has not been addressed. to investigate the role of surfactant in pulmonary inflammation and apoptosis, we instilled natural lung surfactant (survanta) (1.6-12.5 mg) into the rat lungs and determined the number of alveolar macrophages (ams) and apoptotic lung cells. high-dose treatments of survant ... | 2006 | 16534500 |
| incidence of listeria spp. and listeria monocytogenes in a poultry processing environment and in poultry products and their rapid confirmation by multiplex pcr. | volume 60, no. 12, p. 4602: fig. 1 should appear as shown below. fig. 1. incidence of l. monocytogenes and other listeria spp. in a poultry processing environment and in raw and cooked poultry products from march to september 1992. (a) raw (r) and cooked (c) areas within the processing environment. (b) raw and cooked poultry products. (box), l. monocytogenes; (box), listeria spp. (not l. monocytogenes); (symbl), no listeria spp. [this corrects the article on p. 4600 in vol. 60.]. | 1995 | 16534947 |
| dna extraction and pcr methods for the detection of listeria monocytogenes in cold-smoked salmon. | protocols for the specific detection of listeria monocytogenes in cold-smoked salmon were developed. pcr was used as the method of detection. inhibitors of pcr present in the food samples were removed by ether extraction or column purification, or their effect was overcome by the use of tween 20 as an enhancer. these protocols are many times more rapid than conventional detection methodologies and also have the potential for automation. | 1996 | 16535272 |
| use of molecular typing methods to trace the dissemination of listeria monocytogenes in a shrimp processing plant. | volume 62, no. 2, p. 705, column 2, line 5 from bottom: "neutralized with chlorine" should read "chlorine neutralized by the addition of 5 ml of a 1% solution of sodium thiosulfate." page 706, table 1, footnote b: footnote b should read "the designation in parentheses is the area or type of sample collected as indicated in table 3." page 709, tables 3 and 4: tables 3 and 4 should read as shown below. [this corrects the article on p. 705 in vol. 62.]. | 1996 | 16535326 |
| two-dimensional polyacrylamide gel electrophoresis analysis of the acid tolerance response in listeria monocytogenes lo28. | listeria monocytogenes is capable of withstanding low ph after initial exposure to sublethal acidic conditions, a phenomenon termed the acid tolerance response (b. o'driscoll, c. g. m. gahan, and c. hill, appl. environ. microbiol. 62:1693-1698, 1996). treatment of l. monocytogenes lo28 with chloramphenicol during acid adaptation abrogated the protective effect, suggesting that de novo protein synthesis is required for the acid tolerance response. analysis of protein expression during acid adapta ... | 1997 | 16535645 |
| [neonatal listeriosis]. | we present the case of a male with a neonatal listeria monocytogenes infection. its evolution was favorable with intravenous ampicillin and gentamicin. listeriosis is an infrequent cause of neonatal pustulosis. the infection is acquired from the mother after bacteremia with few symptoms (early-onset forms) or while passing through an infected birth canal (late-onset forms). a cytological study and the quick stain technique make fast diagnosis of potentially serious neonatal pustuloses possible. | 2006 | 16540055 |
| concentrations and tracking of listeria monocytogenes strains in a seafood-processing environment using a most-probable-number enrichment procedure and randomly amplified polymorphic dna analysis. | concentrations of environmental microflora and listeria monocytogenes were monitored at multiple environmental locations within a seafood-processing facility over the course of 6 months. concentrations of l. monocytogenes were determined using a most-probable-number (mpn) enrichment procedure. two floor drains had persistent low concentrations of l. monocytogenes (0.03 to >1,100 mpn/cm2). in comparison, concentrations of the other organisms in the drain were much higher (heterotrophic plate coun ... | 2006 | 16541676 |
| influence of different histories of the inoculum on lag phase and growth of listeria monocytogenes in meat models. | the aim of this study was to determine the effect of history of inoculum and preservatives on the lag phase and growth rate of listeria monocytogenes strains in meat products packaged under modified atmosphere conditions. inocula with different histories were added to meat models, and growth rate and lag phase of two strains of l. monocytogenes were measured at 5 and 10 degrees c. the meat model stored at 10 degrees c contained sodium lactate, but the model stored at 5 degrees c did not. the fiv ... | 2006 | 16541682 |
| inactivation of microorganisms in milk and apple cider treated with ultrasound. | nonthermal technologies are emerging as promising alternatives to heat treatment for food processing. ultrasound, defined as sound waves with a frequency greater than 20 khz, has proven bactericidal effects, especially when combined with other microbial-reduction strategies such as mild heating. in this study, ultrasound treatment (sonifier probe at 20 khz, 100% power level, 150 w acoustic power, 118 w/cm2 acoustic intensity) with or without the effect of mild heat (57 degrees c) was effective a ... | 2006 | 16541685 |
| thermal resistance parameters for pathogens in white grape juice concentrate. | the heat resistance of escherichia coli o157:h7, salmonella, and listeria monocytogenes that were in stationary phase, had been exposed to high osmotic pressure, or were acid adapted was evaluated in white grape juice concentrate (58 degrees brix, ph 3.3). the most heat-resistant cells of all three pathogens were those exposed to high osmotic pressure or in stationary phase. unlike in single-strength juices, in concentrate the acid-adapted cells for all three pathogens were less heat resistant t ... | 2006 | 16541686 |
| electron beam and gamma irradiation effectively reduce listeria monocytogenes populations on chopped romaine lettuce. | fresh, chopped romaine lettuce contaminated with a seven-strain cocktail of listeria monocytogenes (in a solution containing approximately 10(8) organisms per ml) that had attained a level of contamination of between 7 and 8 log cfu/g was packaged in 15-g samples. the lettuce was irradiated with a co60 source at 1.15 or 0.51 kgy and then stored at 4 degrees c. in addition, samples contaminated with isolated strains 16397, 0733, and 1992 were subjected to either electron beam irradiation at doses ... | 2006 | 16541687 |
| inactivation of escherichia coli o157:h7, salmonella typhimurium dt104, and listeria monocytogenes on inoculated alfalfa seeds with a fatty acid-based sanitizer. | alfalfa seeds were inoculated with a three-strain cocktail of escherichia coli o157:h7, salmonella enterica subsp. enterica serovar typhimurium dt104, or listeria monocytogenes by immersion to contain approximately 6 to 8 log cfu/g and then treated with a fatty acid-based sanitizer containing 250 ppm of peroxyacid, 1,000 ppm of caprylic and capric acids (emery 658), 1,000 ppm of lactic acid, and 500 ppm of glycerol monolaurate at a reference concentration of 1x. inoculated seeds were immersed at ... | 2006 | 16541689 |
| transfer of listeria monocytogenes during mechanical slicing of turkey breast, bologna, and salami. | a commercial delicatessen slicer was used as the vector for sequential quantitative transfer of listeria monocytogenes (i) from an inoculated slicer blade (approximately 10(8), 10(5), or 10(3) cfu per blade) to 30 slices of uninoculated delicatessen turkey, bologna, and salami, (ii) from inoculated product (approximately 10(8) cfu/cm2) to the slicer, and (iii) from inoculated product (10(8), 10(5), or 10(3) cfu/cm2) to 30 slices of uninoculated product via the slicer blade. cutting force and pro ... | 2006 | 16541694 |
| effect of fat content on infection by listeria monocytogenes in a mouse model. | an estimated 2,500 cases of listeriosis occur annually in the united states. listeriosis is particularly severe among pregnant women and immunocompromised individuals. little is known regarding the effect of the food matrix on the ability of l. monocytogenes to survive in the gastrointestinal tract and cause systemic infection. mice were inoculated with various doses of l. monocytogenes in skim milk, half & half, or whipping cream to determine whether differences in milk fat content influence th ... | 2006 | 16541700 |
| impact of the population of spoilage microflora on the growth of listeria monocytogenes on frankfurters. | approximately 100 cfu/cm2 of a five-strain mixture of listeria monocytogenes was coinoculated onto frankfurters with three different concentrations (10(2), 10(4), and 106 cfu/cm2) of an undefined spoilage microflora derived from commercial frankfurters. the frankfurters were vacuum packaged and stored at 10 degrees c for up to 48 days. the populations of l. monocytogenes, aerobic mesophilic bacteria, lactic acid bacteria, and enterobacteriaceae were determined at various time intervals during st ... | 2006 | 16541704 |
| cerebral listeriosis in an adult freiberger gelding. | listeria monocytogenes infection, although rare in horses, can lead to septicaemia, gastroenteritis and abortion or stillborn foals. equine cerebral listeriosis has been reported, but only in newborn animals. this report describes a freiberger gelding with severe neuronal symptoms of sudden onset. the animal collapsed within 24 h and was humanely killed. necropsy revealed multiple small brown to reddish foci within the brain stem and pons. histopathology demonstrated multifocal suppurative menin ... | 2006 | 16542674 |
| [molecular epidemiology of listeria monocytogenes infections in a health district of madrid in a 3-year period (2001-2003)]. | during 2003, an increase in the number of cases of listeriosis was observed in a tertiary hospital in madrid. the objectives of this study were to review the clinical characteristics of the cases diagnosed from 2001 to 2003 and to investigate clonal relationships among the clinical isolates of listeria monocytogenes. | 2006 | 16545315 |
| distinct roles of tlr2 and the adaptor asc in il-1beta/il-18 secretion in response to listeria monocytogenes. | apoptosis-associated speck-like protein containing a c-terminal caspase recruitment domain (asc) is an adaptor molecule that has recently been implicated in the activation of caspase-1. we have studied the role of asc in the host defense against the intracellular pathogen listeria monocytogenes. asc was found to be essential for the secretion of il-1beta/il-18, but dispensable for il-6, tnf-alpha, and ifn-beta production, in macrophages infected with listeria. activation of caspase-1 was abolish ... | 2006 | 16547271 |
| chronic-contained rupture of an infected aneurysm of the abdominal aorta due to listeria monocytogenes. | we report a case of chronic-contained rupture of an infected aneurysm of the abdominal aorta, from which listeria monocytogenes was cultured. the diagnosis of rupture and retroperitoneal mass was made by computed tomography, whereas fdg -pet diagnosed vessel wall inflammation. the infectious nature only became apparent at surgery. | 2006 | 16547362 |
| lymphocytes are detrimental during the early innate immune response against listeria monocytogenes. | mice deficient in lymphocytes are more resistant than normal mice to listeria monocytogenes infection during the early innate immune response. this paradox remains unresolved: lymphocytes are required for sterilizing immunity, but their presence during the early stage of the infection is not an asset and may even be detrimental. we found that lymphocyte-deficient mice, which showed limited apoptosis in infected organs, were resistant during the first four days of infection but became susceptible ... | 2006 | 16549598 |
| directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. | we have developed a vaccine strategy for generating an attenuated strain of an intracellular bacterial pathogen that, after uptake by professional antigen-presenting cells, does not replicate intracellularly and is readily killed. however, after degradation of the vaccine strain within the phagolysosome, target antigens are released into the cytosol for endogenous processing and presentation for stimulation of cd8(+) effector t cells. applying this strategy to the model intracellular pathogen li ... | 2006 | 16549792 |
| [meningitis due to listeria monocytogenes after spinal anesthesia]. | | 2006 | 16553352 |
| effect of a bacteriocin-activated polythene film on listeria monocytogenes as evaluated by viable staining and epifluorescence microscopy. | to evaluate the effect of a bacteriocin-activated polythene film on resting and growing populations of listeria monocytogenes. | 2006 | 16553731 |
| a newly developed assay to study the minimum inhibitory concentration of satureja spinosa essential oil. | the minimum inhibitory concentration (mic) of satureja spinosa essential oil against staphylococcus aureus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica, salmonella serovar enteritidis pt4 and bacillus cereus was comparatively assessed with an established optical density method as well as a novel impedimetric method. | 2006 | 16553733 |
| single cell variability of l. monocytogenes grown on liver pâté and cooked ham at 7 degrees c: comparing challenge test data to predictive simulations. | the variability in growth between individual listeria monocytogenes cells was investigated on liver pâté and cooked ham. these results were compared to monte carlo simulations based on data collected previously in broths (francois et al., submitted for publication). | 2006 | 16553736 |
| associated roles of hemolysin and p60 protein for the intracellular growth of bacillus subtilis. | hemolysin expressing bacillus subtilis strain (b. subtilis ble/hla) was used as a carrier for listerial protein p60 to study the impact of this protein on bacterial virulence independent of other gene products of listeria monocytogenes. bacillus subtilis ble/hlya exhibited longer cell chains than b. subtilis ble/hlya/iap. recombinant bacillus strains are able to adhere to the mouse macrophage-like j774 and human epithelial-like int407 cell lines. the bacterial number of b. subtilis ble/hlya/iap ... | 2006 | 16553805 |
| characterization of ers, a prfa-like regulator of enterococcus faecalis. | we have identified a transcriptional regulator, named ers (for enterococcal regulator of survival), of enterococcus faecalis, an important opportunistic bacterium commonly recovered from hospitalized patients. ers is a member of the crp/fnr family and is 69% similar to srv, a prfa-like regulator of streptococcus pyogenes implicated in virulence, and is the e. faecalis protein most closely related to prfa, a positive regulator of virulence genes in listeria monocytogenes. in an in vivo-in vitro m ... | 2006 | 16553815 |
| detection of escherichia coli o157:h7, salmonella spp., staphylococcus aureus and listeria monocytogenes in kimchi by multiplex polymerase chain reaction (mpcr). | we developed an mpcr assay for the simultaneous detection, in one tube, of escherichia coli o157:h7, salmonella spp., staphylococcus aureus and listeria monocytogenes using species-specific primers. the mpcr employed the e. coli o157:h7 specific primer stx2a, salmonella spp. specific primer its, s. aureus specific primer cap8a-b and l. monocytogenes specific primer hly. amplification with these primers produced products of 553, 312, 405 and 210 bp, respectively. all pcr products were easily dete ... | 2006 | 16554723 |
| essential role of tnf family molecule light as a cytokine in the pathogenesis of hepatitis. | light is an important costimulatory molecule for t cell immunity. recent studies have further implicated its role in innate immunity and inflammatory diseases, but its cellular and molecular mechanisms remain elusive. we report here that light is upregulated and functions as a proinflammatory cytokine in 2 independent experimental hepatitis models, induced by concanavalin a and listeria monocytogenes. molecular mutagenesis studies suggest that soluble light protein produced by cleavage from the ... | 2006 | 16557300 |
| mechanisms of pathogenesis in listeria monocytogenes infection iv. hepatic carbohydrate metabolism and function in experimental listeriosis. | previous reports have shown alterations in carbohydrate metabolism in mice infected with listeria monocytogenes. this study was undertaken to elucidate mechanisms involved in these changes. female cd-1 mice were injected intraperitoneally with 10(6)l. monocytogenes strain a4413. animals were fasted 12 hr prior to infection, and pooled tissue from several mice was observed at intervals after infection. blood glucose, liver glucose, and liver glycogen decreased within 10 hr after infection. sustai ... | 1970 | 16557711 |
| mutation of listeria monocytogenes after prolonged in vivo survival in diffusion chambers. | listeria monocytogenes remained viable when confined in diffusion chambers in the peritoneal cavities of mice. bacterial survival was associated with mutations. | 1970 | 16557717 |
| effects of listeria monocytogenes hemolysin on phagocytic cells and lysosomes. | the effects of listeria monocytogenes hemolysin on lysosomes and phagocytic cells were investigated. hemolysin caused release of beta-glucuronidase and acid phosphatase from suspensions of rabbit and rat lysosomes prepared from liver homogenates. the degree of lysis was proportional to the concentration of hemolysin added. there appeared to be no significant difference between the sensitivities of rat and rabbit lysosomes to disruption. studies on the effect of ph and temperature on lytic activi ... | 1970 | 16557742 |
| biochemical and immunological effects of listeria monocytogenes hemolysin. | the biochemical and immunological effects of listeria monocytogenes hemolysin in cd-1 mice were studied. intraperitoneal injection of 256 complete hemolytic units (chu) caused a twofold increase in plasma beta-glucuronidase levels but was not lethal. in contrast, 256 or more chu caused 100% lethality in 4 to 5 min when administered intravenously. intravenous administration of 50 chu caused a 10- to 11-fold increase in plasma beta-glucuronidase levels and was lethal for a variable percentage of t ... | 1970 | 16557743 |
| cardiotoxic and lethal effects of listeria monocytogenes hemolysin. | cardiotoxic and lethal effects of listeria monocytogenes hemolysin were studied in cd-1 mice injected with varying doses of hemolysin. intravenous injection of 100 complete hemolytic units (chu) caused 100% lethality within 4 to 5 min. doses ranging from 40 to 50 chu caused death of approximately 50% of the animals. adrenergic blocking agents and antihistamine failed to protect mice against lethality and thereby suggested that death was not due to release of vasoactive agents by hemolysin. plasm ... | 1970 | 16557744 |
| cellular immunity to listeria monocytogenes induced by sensitization and challenge with bovine gamma globulin. | resistance to listeria monocytogenes was demonstrated in guinea pigs undergoing systemic delayed-type hypersensitivity reactions to bovine gamma globulin. | 1970 | 16557768 |
| cellular responses of mice to diffusion chambers i. reactions to intraperitoneal diffusion chambers containing listeria monocytogenes and to bacteria-free chambers. | diffusion chambers made with membranes having a pore size of 0.22 mum were implanted in the peritoneal cavities of mice. chambers that contained no cells induced splenic lymphoreticular hyperplasia and a proliferation of fibroblasts around the chambers. when the chambers contained the bacterium, listeria monocytogenes, there was strong and continuous chemotaxis of phagocytic cells to the membrane surface. the tendency to incite fibrosis around the chambers containing bacteria produced a tissue r ... | 1970 | 16557809 |
| bactericidal activity of human macrophages: analysis of factors influencing the killing of listeria monocytogenes. | a technique is described for the measurement of listericidal activity of human macrophages grown from blood monocytes. phagocytosis of listeria monocytogenes was inhibited by a glycolytic poison (naf) but was unaffected by anaerobic conditions, cyanide, or 2,4-dinitrophenol (dnp). killing by macrophages was slower than that by neutrophils, and listeria phagocytized by macrophages began to synthesize deoxyribonucleic acid within 3 hr of the time of ingestion. differentiated macrophages ingested a ... | 1970 | 16557814 |
| oral immunization in experimental salmonellosis ii. characteristics of the immune response to temperature-sensitive mutants given by oral and parenteral routes. | a temperature-sensitive mutant of salmonella enteritidis, selected because of its inability to proliferate normally at 37 c, has been used as a living vaccine in mice. when given parenterally or orally, it confers a high degree of resistance against otherwise lethal s. enteritidis infections given intraperitoneally or by mouth. in contrast to most other effective living salmonella vaccines, the temperature-sensitive mutant survives for only short periods in mouse tissues. although the vaccine pr ... | 1970 | 16557818 |
| drug potentiation of macrophage function. | the mechanism by which macrophages kill facultative intracellular parasites is not known. a test system is described utilizing human macrophages derived from blood monocytes and the opportunistic pathogen listeria monocytogenes. in this system, bacterial killing was impaired under hypoxic conditions. under aerobic conditions, addition of clofazimine (b663), a phenazine derivative effective in the treatment of human leprosy, potentiated the killing of listeria by macrophages. potentiation occurre ... | 1970 | 16557883 |
| extracellular antigens from listeria monocytogenes i. purification and resolution of hemolytic and lipolytic antigens from culture filtrates of listeria monocytogenes. | two antigens were purified from culture filtrates of listeria monocytogenes 7973 by the following procedure: (i) acid precipitation with 4 n hcl at ph 3.7, (ii) sephadex g-75 column fractionation, (iii) diethylaminoethyl-sephadex a50 batchwise adsorption, and (iv) rechromatography on sephadex g-75. this procedure resulted in the resolution of two distinct antigens. one antigen, designated a hemolytic antigen because of its ability to lyse erythrocytes from a variety of species, had a specific ac ... | 1971 | 16558022 |
| effects of dextran sulfate 500 on cell-mediated resistance to infection with listeria monocytogenes in mice. | injection of dextran sulfate 500 caused loss of antibacterial resistance. mice became more susceptible to an infection with listeria monocytogenes and were unable to develop antilisterial immunity after both active and passive immunization with passively administered spleen cells from listeria-immune donors. indirect evidence suggests that the phagocytic component of cell-mediated resistance to bacterial infection is the site of attack of dextran sulfate. | 1974 | 16558097 |
| induction of resistance by listeria monocytogenes cell wall fraction. | a crude cell wall fraction of listeria monocytogenes was prepared by sonic disruption and differential centrifugation of viable, washed cultures. when injected into mice, this sterile, crude cell wall fraction protected mice against an intraperitoneal challenge with 18 to 85 50% mean lethal dose of l. monocytogenes. resistance was greatly enhanced when bacterial endotoxin (lipopolysaccharide) was injected along with the cell wall fraction. resistance was measured both by enumerating the bacteria ... | 1974 | 16558105 |
| the flagellation of listeria monocytogenes. | | 1944 | 16560812 |
| susceptibility of the sage brush vole, lagurus curtatus, to listeria monocytogenes. | | 1965 | 16562034 |
| specificity of acquired resistance produced by immunization with listeria monocytogenes and listeria fractions. | mice were immunized with 1.0 mg of an attenuated strain of listeria monocytogenes to determine the period of protection afforded by this strain when the mice were challenged intravenously with 5 mld of listeria. protection appeared 2 days after immunization and was still apparent 4 weeks after immunization. if the challenge dose was decreased to 1 mld, protection was apparent at 10 weeks. mice immunized with a comparable dose of mycobacterial cells and challenged intravenously with 1 mld of list ... | 1969 | 16562159 |
| autoinducer-2 bioassay is a qualitative, not quantitative method influenced by glucose. | autoinducer-2 (ai-2) is a cell-to-cell signaling molecule which is thought to be utilized for quorum sensing processes by a variety of prokaryotic species. this molecule is usually detected using a so-called autoinducer bioassay, which relies on the ability of a vibrio harveyi reporter strain to produce light in response to ai-2. however, as previously reported, the presence of glucose in the sample can inhibit the bioluminescence of the reporter strain, a fact that is often ignored by investiga ... | 2006 | 16564586 |