| prevalence and antimicrobial resistance in salmonella enterica isolated from broiler chickens, pigs and meat products in thailand-cambodia border provinces. | this study aimed to examine the prevalence and antimicrobial resistance (amr) of salmonella isolates from broiler chickens, pigs and their associated meat products in the thailand-cambodia border provinces. a total of 941 samples were collected from pigs and broiler chickens at slaughter houses and from carcasses at local fresh markets in sa kaeo, thailand (n = 554) and banteay meanchey, cambodia (n = 387) in 2014 and 2015. from these samples, 345 salmonella isolates were collected from sa keao ... | 2017 | 28042666 |
| short communication: characterization of salmonella phages from dairy calves on farms with history of diarrhea. | salmonella enterica can cause disease and mortality in calves. this pathogen is also a zoonosis that can be transmitted by animal contact or by food. the prevalence of salmonella in dairy farms has been reported to range from 0 to 64%, and, due to the diversity of salmonella serovars that can be circulating, salmonella is an important concern for dairy production. bacteriophages that infect salmonella have been documented to be abundant and widely distributed in the dairy environment. the curren ... | 2017 | 28041722 |
| outbreak of salmonella oslo infections linked to persian cucumbers - united states, 2016. | in april 2016, pulsenet, the national molecular subtyping network for foodborne disease surveillance, detected a multistate cluster of salmonella enterica serotype oslo infections with an indistinguishable pulsed-field gel electrophoresis (pfge) pattern (xbai pfge pattern oslx01.0090).* this pfge pattern was new in the database; no previous infections or outbreaks have been identified. cdc, state and local health and agriculture departments and laboratories, and the food and drug administration ... | 2016 | 28033312 |
| fowl typhoid in laying hens cause hepatic oxidative stress. | the aim of this study was to analyses nitric oxide, antioxidant status, and oxidative profile in the liver of laying hens naturally infected by salmonella enterica subsp enterica serovar gallinarum (s. gallinarum). the nitrite/nitrate (nox), reactive oxygen species (ros), thiobarbituric acid-reactive substances (tbars), catalase (cat), glutathione reductase (gr), glutathione peroxidase (gpx) and glutathione s-transferase (gst) activities were measured in liver samples, as well the biomarkers of ... | 2017 | 28027943 |
| laboratory investigation of <i>salmonella enterica</i> serovar poona outbreak in california: comparison of pulsed-field gel electrophoresis (pfge) and whole genome sequencing (wgs) results. | recently, salmonella enterica serovar poona caused a multistate outbreak, with 245 out of 907 cases occurring in california. we report a comparison of pulsed-field gel electrophoresis (pfge) results with whole genome sequencing (wgs) for genotyping of salmonella poona isolates. | 2016 | 28018748 |
| draft genome sequence of a multidrug-resistant cmy-2-producing salmonella enterica subsp. enterica serovar minnesota st3088 isolated from chicken meat. | | 2017 | 28017672 |
| increased secretion of exopolysaccharide and virulence potential of a mucoid variant of salmonella enterica serovar montevideo under environmental stress. | during an investigation to increase the recovery of salmonella enterica from oregano, an increased expression of exopolysaccharide was induced in salmonella serovar montevideo. the atypical mucoid (sal242s) and the non-mucoid (sal242) strains of montevideo were compared and characterized using various methods. serotyping analysis demonstrated that both strains are the same serovar montevideo. electron microscopy (em) of cultured sal242s cells revealed the production of a prominent eps-like struc ... | 2017 | 28012986 |
| antimicrobial resistance patterns of bovine salmonella enterica isolates submitted to the wisconsin veterinary diagnostic laboratory: 2006-2015. | salmonellosis on the dairy continues to have a significant effect on animal health and productivity and in the united states. additionally, salmonella enterica ssp. enterica causes an estimated 1.2 million cases of human illness annually. contributing to the morbidity and mortality in both human and domestic animal species is emergence of antimicrobial resistance by salmonella species and increased incidence of multidrug-resistant isolates. this study describes serotype distribution and the anti ... | 2017 | 28012630 |
| longitudinal study of salmonella 1,4,[5],12:i:- shedding in five australian pig herds. | the shedding patterns of salmonella spp. and mlva profiles of salmonella enterica subspecies enterica (i) serotype 1,4,[5],12:i:- were monitored in a 12-month longitudinal observational study of five pig herds to inform management; provide indications of potential hazard load at slaughter; and assist evaluation of mlva for use by animal and public health practitioners. twenty pooled faecal samples, stratified by age group, were collected quarterly. when salmonella was cultured, multiple colonies ... | 2017 | 28010904 |
| detection of salmonella enterica serovar montevideo and newport in free-ranging sea turtles and beach sand in the caribbean and persistence in sand and seawater microcosms. | salmonellae are gram-negative zoonotic bacteria that are frequently part of the normal reptilian gastrointestinal flora. the main objective of this project was to estimate the prevalence of non-typhoidal salmonella enterica in the nesting and foraging populations of sea turtles on st. kitts and in sand from known nesting beaches. results suggest a higher prevalence of salmonella in nesting leatherback sea turtles compared with foraging green and hawksbill sea turtles. salmonella was cultured fro ... | 2016 | 28009107 |
| prevalence and antimicrobial resistance of salmonella enterica serovar indiana in china (1984-2016). | salmonella enterica serovar indiana, first described in 1955, is generally regarded as having a low frequency worldwide with outbreaks of gastroenteritis and abortions described in north america and europe. in china, s. indiana was first reported in 1984 and in the subsequent 71 surveys in 35 cities/municipalities from 18 provinces, 70% of which were after 2012, s. indiana has been shown to have become widely prevalent in people, animals, food and the environment around abattoirs and meat proces ... | 2016 | 28009105 |
| high occurrence and unusual serotype diversity of non-typhoidal salmonella in non-clinical niches, angola. | non-typhoidal salmonella is an important burden, particularly in developing countries of the african region. we report for the first time in angola, a sub-saharan african country with commercial/travel relationships with europe, an unexpectedly high occurrence of salmonella (n = 12/63, 19%) from a high diversity of sources, particularly farm and wild animals. the detection of diverse serotypes (n = 12), involving putative new s. enterica subsp. salamae serotypes, is also of note, reinforcing the ... | 2017 | 28004619 |
| complete genome sequence of new bacteriophage phie142, which causes simultaneously lysis of multidrug-resistant escherichia coli o157:h7 and salmonella enterica. | the emergence of antibiotic-resistant foodborne bacteria is a global health problem that requires immediate attention. bacteriophages are a promising biotechnological alternative approach against bacterial pathogens. however, a detailed analysis of phage genomes is essential to assess the safety of the phages prior to their use as biocontrol agents. therefore, here we report the complete genome sequence of bacteriophage phie142, which is able to lyse salmonella and multidrug-resistant escherichi ... | 2016 | 27999624 |
| cytochrome bd-dependent bioenergetics and antinitrosative defenses in salmonella pathogenesis. | in the course of an infection, salmonella enterica occupies diverse anatomical sites with various concentrations of oxygen (o2) and nitric oxide (no). these diatomic gases compete for binding to catalytic metal groups of quinol oxidases. enterobacteriaceae express two evolutionarily distinct classes of quinol oxidases that differ in affinity for o2 and no as well as stoichiometry of h(+) translocated across the cytoplasmic membrane. the investigations presented here show that the dual function o ... | 2016 | 27999164 |
| characterization of o-antigen delivered by generalized modules for membrane antigens (gmma) vaccine candidates against nontyphoidal salmonella. | invasive nontyphoidal salmonella disease (ints) is a leading cause of death and morbidity in africa. the most common pathogens are salmonella enterica serovars typhimurium and enteritidis. the o-antigen portion of their lipopolysaccharide is a target of protective immunity and vaccines targeting o-antigen are currently in development. here we investigate the use of generalized modules for membrane antigens (gmma) as delivery system for s. typhimurium and s. enteritidis o-antigen. gram-negative b ... | 2017 | 27998639 |
| the crispr-cas system presents multiple transcriptional units including antisense rnas that are expressed in minimal medium and up-regulated by ph in salmonella enterica serovar typhi. | the crispr-cas system is involved in bacterial immunity, virulence, gene regulation, biofilm formation and sporulation. in salmonella enterica serovar typhi this system consists of five transcriptional units including antisense rnas. it was determined that these genetic elements are expressed in minimal medium and are up-regulated by ph. in addition, a transcriptional characterization of cas3 and ascse2-1 is included herein. | 2016 | 27995873 |
| role of t3ss-1 sipd protein in protecting mice against non-typhoidal salmonella typhimurium. | salmonella enterica species are enteric pathogens that cause severe diseases ranging from self-limiting gastroenteritis to enteric fever and sepsis in humans. these infectious diseases are still the major cause of morbidity and mortality in low-income countries, especially in children younger than 5 years and immunocompromised adults. vaccines targeting typhoidal diseases are already marketed, but none protect against non-typhoidal salmonella. the existence of multiple non-typhoidal salmonella s ... | 2016 | 27992422 |
| detection of salmonella enterica in meat in less than 5 hours by a low-cost and noncomplex sample preparation method. | salmonella is recognized as one of the most important foodborne bacteria and has wide health and socioeconomic impacts worldwide. fresh pork meat is one of the main sources of salmonella, and efficient and fast methods for detection are therefore necessary. current methods for salmonella detection in fresh meat usually include >16 h of culture enrichment, in a few cases <12 h, thus requiring at least two working shifts. here, we report a rapid (<5 h) and high-throughput method for screening of s ... | 2017 | 27986726 |
| salmonella enterica serotype napoli is the first cause of invasive nontyphoidal salmonellosis in lombardy, italy (2010-2014), and belongs to typhi subclade. | salmonella enterica serotype napoli (s. napoli) is currently emerging in europe and particularly in italy, where in 2014 it caused a large outbreak associated with elevated rates of bacteremia. however, no study has yet investigated its invasive ability and phylogenetic classification. here, we show that between 2010 and 2014, s. napoli was the first cause of invasive salmonellosis affecting 40 cases out of 687 (invasive index: 5.8%), which is significantly higher than the invasive index of all ... | 2017 | 27982698 |
| genome sequences of multidrug-resistant salmonella enterica subsp. enterica serovar infantis strains from broiler chicks in hungary. | three strains of salmonella enterica serovar infantis isolated from healthy broiler chickens from 2012 to 2013 have been sequenced. comparison of these and previously published s infantis genome sequences of broiler origin in 1996 and 2004 will provide new insight into the genome evolution and recent spread of s infantis in poultry. | 2016 | 27979950 |
| cyclic amp inhibits the activity and promotes the acetylation of acetyl-coa synthetase through competitive binding to the atp/amp pocket. | the high-affinity biosynthetic pathway for converting acetate to acetyl-coenzyme a (acetyl-coa) is catalyzed by the central metabolic enzyme acetyl-coenzyme a synthetase (acs), which is finely regulated both at the transcriptional level via cyclic amp (camp)-driven trans-activation and at the post-translational level via acetylation inhibition. in this study, we discovered that camp directly binds to salmonella enterica acs (seacs) and inhibits its activity in a substrate-competitive manner. in ... | 2017 | 27974467 |
| salmonella enterica subspecies arizonae infection of adult patients in southern taiwan: a case series in a non-endemic area and literature review. | the majority of salmonella arizonae human infections have been reported in southwestern united states, where rattlesnake-based products are commonly used to treat illness; however, little is known in non-endemic areas. we reviewed and analyzed the clinical manifestations and treatment outcomes in adult patients with s. arizonae infection at our institution. | 2016 | 27938338 |
| serotypes and antimicrobial resistance in salmonella enterica recovered from clinical samples from cattle and swine in minnesota, 2006 to 2015. | salmonellosis remains one of the leading causes of foodborne disease worldwide despite preventive efforts at various stages of the food production chain. the emergence of multi-drug resistant (mdr) non-typhoidal salmonella enterica represents an additional challenge for public health authorities. food animals are considered a major reservoir and potential source of foodborne salmonellosis; thus, monitoring of salmonella strains in livestock may help to detect emergence of new serotypes/mdr pheno ... | 2016 | 27936204 |
| purification and antigenic detection of o-specific polysaccharides of salmonella enterica serovar paratyphi a isolate from pakistan: an emerging threat. | paratyphoid fever caused by salmonella enterica serovar paratyphi a is becoming a serious health problem in asian countries particularly pakistan, china and india and situation is aggravated by current unavailability of a licensed vaccine. this study was designed to purify the o-specific polysaccharides (osp) produced by an isolate of salmonella paratyphi a from pakistan and detect antigenicity of extracted lipopolysaccharide (lps) and purified osp pioneerly in south asian region as candidate fo ... | 2016 | 27933236 |
| salmonella co-opts host cell chaperone-mediated autophagy for intracellular growth. | salmonella enterica are invasive intracellular pathogens that replicate within a membrane-bound compartment inside infected host cells known as the salmonella-containing vacuole. how salmonella obtains nutrients for growth within this intracellular niche despite the apparent isolation is currently not known. recent studies have indicated the importance of glucose and related carbon sources for tissue colonization and intracellular proliferation within host cells during salmonella infections, alt ... | 2017 | 27932462 |
| high-resolution ph imaging of living bacterial cells to detect local ph differences. | protons are utilized for various biological activities such as energy transduction and cell signaling. for construction of the bacterial flagellum, a type iii export apparatus utilizes atp and proton motive force to drive flagellar protein export, but the energy transduction mechanism remains unclear. here, we have developed a high-resolution ph imaging system to measure local ph differences within living salmonella enterica cells, especially in close proximity to the cytoplasmic membrane and th ... | 2016 | 27923921 |
| the type iii secretion system effector sptp of salmonella enterica serovar typhi. | strains of the various salmonella enterica serovars cause gastroenteritis or typhoid fever in humans, with virulence depending on the action of two type iii secretion systems (salmonella pathogenicity island 1 [spi-1] and spi-2). sptp is a salmonella spi-1 effector, involved in mediating recovery of the host cytoskeleton postinfection. sptp requires a chaperone, sicp, for stability and secretion. sptp has 94% identity between s. enterica serovar typhimurium and s typhi; direct comparison of the ... | 2017 | 27920299 |
| antimicrobial resistance and molecular subtypes of salmonella enterica serovar typhi isolates from kolkata, india over a 15 years period 1998-2012. | typhoid fever, caused by salmonella enterica serovar typhi (s. typhi), remains an unresolved public health problem in india. emergence of antimicrobial resistant strains poses a great concern for typhoid treatment and influences reshaping of current s. typhi population. we included representative s. typhi strains (n=164) from retrospective studies, both community and hospital based, conducted at national institute of cholera and enteric diseases, kolkata during 15 years period (1998-2012) to ana ... | 2017 | 27916384 |
| association between pigs with high caecal salmonella loads and carcass contamination. | contaminated pork is a significant source of foodborne salmonella infections. pork is contaminated at the slaughterhouse; however, the mechanisms driving salmonella contamination of carcasses are still poorly understood. the aim of this study was to investigate whether the amount of salmonella carried by slaughtered pigs in their guts has an influence on carcass contamination. on that account, we tested whether the number of carcasses contaminated during a slaughter day was associated with the p ... | 2017 | 27914322 |
| cutting edge: lymphotoxin signaling is essential for clearance of salmonella from the gut lumen and generation of anti-salmonella protective immunity. | the immunological components that control resolution of salmonella infection and successful vaccination are poorly defined. in a model of chronic gastrointestinal infection, we observed that the lymphotoxin (lt) pathway is essential for the clearance and resolution of primary infection of attenuated salmonella enterica typhimurium strain sl3261 δaroa using gnotobiotic mice, we show that ltβ receptor (ltβr) signaling and the microbiota are required to promote clearance of attenuated s. enterica t ... | 2017 | 27913631 |
| interaction between salmonella and schistosomiasis: a review. | the interaction between schistosomiasis and salmonella is a particularly important issue in africa, where dual infection by the parasite and the bacterium are likely common. in this review, the ways in which schistosomiasis affects human biology as it relates to salmonella are described. those who are infected by both organisms experience reduced immunological functioning, exhibit irreversible organ damage due to prolonged schistosomiasis infection, and become latent carriers of salmonella enter ... | 2016 | 27907208 |
| antimicrobial susceptibility to azithromycin among salmonella enterica typhi and paratyphi a isolates from india. | decreased ciprofloxacin susceptibility (dcs) and multidrug resistance in typhoidal salmonella isolates in areas of endemicity are significant therapeutic problems. guidelines for azithromycin disc diffusion and mic interpretive criteria for salmonella enterica serovar typhi were published recently by the clinical and laboratory standards institute in 2015. we investigated the antimicrobial susceptibility pattern of azithromycin in 100 isolates of salmonella typhi (n=80), paratyphi a (n=18) and b ... | 2016 | 27902389 |
| a case of clinical and microbiological failure of azithromycin therapy in salmonella enterica serotype typhi despite low azithromycin mic. | typhoid fever remains a serious problem in many developing countries. due to resistance to multiple first line drugs, azithromycin has evolved as an important drug in the treatment of typhoid. while therapy with azithromycin is highly effective, no clinically validated mean inhibitory concentration (mic) break points or disc diffusion cutoff guidelines are available so far. we describe an indian adult with clinical and microbiological failure to azithromycin despite low azithromycin mic. | 2017 | 27894983 |
| a comparative evaluation of different diagnostic modalities in the diagnosis of typhoid fever using a composite reference standard: a tertiary hospital based study in central india. | enteric fever, caused by salmonella spp. is a major cause of morbidity and mortality worldwide and endemic in many developing countries including india and other south-east asian countries. blood culture is regarded as the gold standard for diagnosis. currently, the standard serological method is tube agglutination with moderate sensitivity and specificity. dot blot assay detecting igm and igg antibodies to a specific 50kd outer membrane protein (omp) antigen of salmonella spp. is a simple, reli ... | 2016 | 27891335 |
| variability in the adaptive acid tolerance response phenotype of salmonella enterica strains. | the objective of this study was the assessment of the stationary-phase, low-ph-inducible acid tolerance response (atr) of different salmonella enterica strains. for this purpose, 30 strains of the pathogen were grown in tryptone soy broth in the absence (non-adapted cultures) and presence (1% w/v; acid-adapted cultures) of glucose, and then subjected to 4-h acid challenge trials at ph 3.0. surviving populations of each strain were determined at 1-h intervals, and the weibull model was fitted to ... | 2017 | 27889173 |
| inactivation of salmonella enterica in chicken feces on the surface of eggshells by simultaneous treatments with gaseous chlorine dioxide and mild wet heat. | the aim of this study was to investigate the lethal effects of simultaneous treatments with gaseous chlorine dioxide (clo2) and mild wet heat (55 °c at 100% relative humidity [rh]) on salmonella enterica in chicken feces on the surface of eggshells. gaseous clo2 production decreased significantly (p ≤ 0.05) as the rh (23, 43, 68, 85, and 100%) at 25 °c was increased. the lethality of gaseous clo2 against s. enterica in feces on eggshells increased significantly (p ≤ 0.05) as rh increased. for ex ... | 2017 | 27889149 |
| in silico clustering of salmonella global gene expression data reveals novel genes co-regulated with the spi-1 virulence genes through hild. | a wide variety of salmonella enterica serovars cause intestinal and systemic infections to humans and animals. salmonella patogenicity island 1 (spi-1) is a chromosomal region containing 39 genes that have crucial virulence roles. the arac-like transcriptional regulator hild, encoded in spi-1, positively controls the expression of the spi-1 genes, as well as of several other virulence genes located outside spi-1. in this study, we applied a clustering method to the global gene expression data of ... | 2016 | 27886269 |
| hyperinvasiveness of salmonella enterica serovar choleraesuis linked to hyperexpression of type iii secretion systems in vitro. | salmonella enterica serovars choleraesuis and typhimurium are among the non-typhoid salmonella serovars that are important zoonotic pathogens. in clinical observation, s. typhimurium typically causes diarrheal diseases; however, s. choleraesuis shows high predilection to cause bacteremia. the mechanism why s. choleraesuis is more invasive to humans remains unknown. in this study, we compared the s. typhimurium lt2 and s. choleraesuis sc-b67 proteomes through stable isotope labeling of amino acid ... | 2016 | 27886215 |
| rapid detection of salmonella in raw chicken breast using real-time pcr combined with immunomagnetic separation and whole genome amplification. | we presented the first attempt to combine immunomagnetic separation (ims), whole genome amplification by multiple displacement amplification (mda) and real-time pcr for detecting a bacterial pathogen in a food sample. this method was effective in enabling real-time pcr detection of low levels of salmonella enterica serotype enteritidis (se) (∼10 cfu/g) in raw chicken breast without culture enrichment. in addition, it was able to detect refrigeration-stressed se cells at lower concentrations (∼0. ... | 2017 | 27884771 |
| draft genome sequence of salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) strain crjjgf_00165 (phylum gammaproteobacteria). | here, we report a 4.78-mb draft genome sequence of the salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) strain crjjgf_00165 [also called s. enterica subsp. iiib serovar 61:k:1,5,(7) strain crjjgf_00165], isolated from ground beef in 2007. | 2016 | 27881547 |
| salmonella enterica serovar pomona infection in farmed juvenile american alligators ( alligator mississippiensis). | a fatal epizootic of salmonellosis occurred in farmed juvenile american alligators in louisiana. six animals were examined. gross lesions included severe fibrinonecrotizing enterocolitis, necrotizing splenitis, coelomic effusion, and perivisceral and pulmonary edema. microscopic examination revealed severe necrotizing enterocolitis and splenitis with intralesional bacteria and pneumocyte necrosis with fibrin thrombi. salmonella enterica serovar pomona was isolated from intestine and lung. clinic ... | 2017 | 27879442 |
| salmonella enteritidis effector avra stabilizes intestinal tight junctions via the jnk pathway. | salmonella pathogenesis studies to date have focused on salmonella typhimurium, and the pathogenesis of a second major serotype, salmonella enteritidis, is poorly understood. salmonella spp. possess effector proteins that display biochemical activities and modulate host functions. here, we generated a deletion mutant of the effector avra, s.e-avra(-), and a plasmid-mediated complementary strain, s.e-avra(-)/pavra(+) (s.e-avra(+)), in s. enteritidis. using in vitro and in vivo infection models, w ... | 2016 | 27875307 |
| current aspects of salmonella contamination in the us poultry production chain and the potential application of risk strategies in understanding emerging hazards. | one of the leading causes of foodborne illness in poultry products is salmonella enterica. salmonella hazards in poultry may be estimated and possible control methods modeled and evaluated through the use of quantitative microbiological risk assessment (qmra) models and tools. from farm to table, there are many possible routes of salmonella dissemination and contamination in poultry. from the time chicks are hatched through growth, transportation, processing, storage, preparation, and finally co ... | 2017 | 27869522 |
| vi capsular polysaccharide: synthesis, virulence, and application. | vi capsular polysaccharide, a linear homopolymer of α-1,4-linked n-acetylgalactosaminuronate, is characteristically produced by salmonella enterica serovar typhi. the vi capsule covers the surface of the producing bacteria and serves as an virulence factor via inhibition of complement-mediated killing and promoting resistance against phagocytosis. furthermore, vi also represents a predominant protective antigen and plays a key role in the development of vaccines against typhoid fever. herein, we ... | 2016 | 27869515 |
| salad leaf juices enhance salmonella growth, colonization of fresh produce, and virulence. | we show in this report that traces of juices released from salad leaves as they become damaged can significantly enhance colonization of salad leaves by salmonella enterica salad juices in water increased salmonella growth by 110% over the level seen with the unsupplemented control and in host-like serum-based media by more than 2,400-fold over control levels. in serum-based media, salad juices induced growth of salmonella via provision of fe from transferrin, and siderophore production was foun ... | 2017 | 27864173 |
| nasal immunization with inhibin dna vaccine delivered by attenuated salmonella choleraesuis for improving ovarian responses and fertility in cross-bred buffaloes. | this study was conducted to determine the effect of immunization with inhibin dna vaccine delivered by attenuated salmonella choleraesuis on ovarian responses and fertility in cross-bred buffaloes. a total of 134 cross-bred buffaloes were divided into four groups: groups t1 (n = 34), t2 (n = 35) and t3 (n = 31) were nasal immunized twice a day with 10 ml of 1 × 10(10) cfu/ml of the c501 (pvax-asd-is) vaccine for 5, 3 and 1 day, respectively. group c (n = 34) was nasal immunized with 10 ml pbs f ... | 2017 | 27862394 |
| salmonella in swine: microbiota interactions. | for the important foodborne pathogen salmonella enterica to cause disease or persist in pigs, it has evolved an intricate set of interactions between itself, the host, and the indigenous microflora of the host. s. enterica must evade the host's immune system and must also overcome colonization resistance mediated by the pig's indigenous microflora. the inflammatory response against s. enterica provides the bacteria with unique metabolites and is thus exploited by s. enterica for competitive adva ... | 2017 | 27860494 |
| multidrug-resistant outbreak-associated salmonella strains in irrigation water from the metropolitan region, chile. | salmonella enterica (s. enterica) is the main cause of foodborne diseases in the chilean population. with the aim of characterizing the presence of s. enterica in bodies of water, samples from 40 sources were obtained, including rivers and irrigation canals used by agricultural farms in the most populated regions of chile. as result, 35 s. enterica isolates belonging to several serotypes were detected, with the highest frequency represented by typhimurium and enteritidis. all strains showed phen ... | 2016 | 27860367 |
| genome sequences of two salmonella enterica serovar kentucky isolates recovered from poultry carcasses in the united states. | we report here the draft genome sequences of two salmonella enterica serovar kentucky eburstgroup 15 isolates collected from poultry carcasses in georgia (usa). | 2016 | 27856587 |
| biocide tolerance and antibiotic resistance in salmonella isolates from hen eggshells. | the aim of the present study was to determine biocide tolerance and antibiotic resistance in salmonella isolates from hen eggshells. a total of 39 isolates from hen eggshells, identified as either salmonella spp. or salmonella enterica according to 16s rdna sequencing, were selected for biocide tolerance. isolates with minimum inhibitory concentrations (mics) above the wild-type mics were considered to be biocide tolerant: benzalkonium chloride (bc, 7.7%), cetrimide (ct, 7.7%), hexadecylpyridini ... | 2017 | 27841937 |
| high resolution melting (hrm) analysis as a new tool for rapid identification of salmonella enterica serovar gallinarum biovars pullorum and gallinarum. | salmonella enterica serovar gallinarum biovars pullorum and gallinarum represent the most common causative agents of chicken salmonellosis, which result in high mortality and morbidity throughout the world. it is difficult and laborious to discriminate these diseases based on biochemical or phenotypic methods. herein, we report the development of a single nucleotide polymorphism (snp) pcr-high resolution melt (pcr-hrm) assay for the detection and discrimination of both s pullorum and s gallinaru ... | 2016 | 27837117 |
| distribution trends & antibiogram pattern of salmonella enterica serovar newport in india. | salmonellosis is a major public health concern worldwide. besides typhoidal salmonellae, infections due to non-typhoidal serovars of salmonella are also associated with high morbidity and mortality leading to huge economic losses. among non-typhoidal serovars, salmonella newport has been reported as a major cause of foodborne infections resulting in outbreaks due to consumption of contaminated food items. little data related to this serovar are available from india leading to the scarcity of inf ... | 2016 | 27834330 |
| spatial distribution and risk factors associated with salmonella enterica in pigs. | the importance of pork in the transmission of salmonella spp. to humans has led to the development of control programmes worldwide. for this, knowledge on the epidemiology of the infection in the production system is fundamental to the efficacy of the regulations. our objective was to determine the prevalence and spatial distribution of salmonella-infected farms in the central region of argentina, and to identify the predominant serotypes and epidemiological factors associated with an increased ... | 2017 | 27834167 |
| the salmonella effector sted mediates march8-dependent ubiquitination of mhc ii molecules and inhibits t cell activation. | the spi-2 type iii secretion system (t3ss) of intracellular salmonella enterica translocates effector proteins into mammalian cells. infection of antigen-presenting cells results in spi-2 t3ss-dependent ubiquitination and reduction of surface-localized mature mhc class ii (mmhcii). we identify the effector sted as required and sufficient for this process. in mel juso cells, sted localized to the golgi network and vesicles containing the e3 ubiquitin ligase march8 and mmhcii. sted caused march8-d ... | 2016 | 27832589 |
| fe(3+)-dependent epistasis between the cpxr-activated loci and the pmra-activated lps modification loci in salmonella enterica. | bacteria utilize varying combinations of two-component regulatory systems, many of which respond and adapt closely to stress conditions, thus expanding their niche steadily. while mechanisms of recognition and avoidance of the specific fe(3+) signal by the pmra/pmrb system is well understood, those of the cpxr/cpxa system are more complex because they can be induced by various stress conditions, which, in turn, expresses a variety of phenotypes. here, we highlight another aspect of the cpxr/cpxa ... | 2017 | 27829584 |
| is the evolution of salmonella enterica subsp. enterica linked to restriction-modification systems? | salmonella enterica subsp. enterica bacteria are highly diverse foodborne pathogens that are subdivided into more than 1,500 serovars. the diversity is believed to result from mutational evolution, as well as intra- and interspecies recombination that potentially could be influenced by restriction-modification (rm) systems. the aim of this study was to investigate whether rm systems were linked to the evolution of salmonella enterica subsp. enterica. the study included 221 salmonella enterica ge ... | 2017 | 27822532 |
| molecular characterization of salmonella serovars anatum and ealing associated with two historical outbreaks, linked to contaminated powdered infant formula. | powdered infant formula (pif) is not intended to be produced as a sterile product unless explicitly stated and on occasion may become contaminated during production with pathogens such as salmonella enterica. this retrospective study focused on two historically reported salmonellosis outbreaks associated with pif from the united kingdom and france, in 1985 and 1996/1997. in this paper, the molecular characterization of the two outbreaks associated salmonella serovars anatum and ealing is reporte ... | 2016 | 27818652 |
| complete whole-genome sequence of salmonella enterica subsp. enterica serovar java nctc5706. | salmonellae are a significant cause of morbidity and mortality globally. here, we report the first complete genome sequence for salmonella enterica subsp. enterica serovar java strain nctc5706. this strain is of historical significance, having been isolated in the pre-antibiotic era and was deposited into the national collection of type cultures in 1939. | 2016 | 27811100 |
| disseminated infections with antibiotic-resistant non-typhoidal salmonella strains: contributions of host and pathogen factors. | non-typhoidal salmonella enterica serovars (nts) are generally associated with gastroenteritis; however, the very young and elderly, as well as individuals with compromised immunity, are at risk of developing disseminated infection that can manifest as bacteremia or focal infections at systemic sites. disseminated nts infections can be fatal and are responsible for over 600 000 deaths annually. most of these deaths are in sub-saharan africa, where multidrug-resistant nts clones are currently cir ... | 2016 | 27765795 |
| identification of mhc-bound peptides from dendritic cells infected with salmonella enterica strain sl1344: implications for a nontyphoidal salmonella vaccine. | worldwide salmonella enterica infections result in substantial morbidity and mortality and are the major cause of infant bacteremia in sub-saharan africa. diseases caused by salmonella are treatable with antibiotics, but successful antibiotic treatment has become difficult due to antimicrobial resistance and collateral effects on the microbiome. an effective vaccine together with public health efforts may be a better strategy to control these infections. protective immunity against salmonella de ... | 2017 | 27802388 |
| prevalence and characteristics of salmonella isolated from free-range chickens in shandong province, china. | compared with chickens raised in intensively managed breeding farms, free-range chickens in china are quite popular due to lower breeding density and less antibiotics usage. however, investigations about salmonella enterica from free-range chickens are quite rare. the aim of the present study was to investigate prevalence and characteristics of salmonella in free-range chickens in shandong province, china. during the period of august and november 2015, 300 fresh fecal swabs from different broile ... | 2016 | 27800493 |
| influence of pigskin on salmonella contamination of pig carcasses and cutting lines in an italian slaughterhouse. | ninety pig carcasses and twenty one food contact surfaces (fcss) were tested for salmonella in a slaughterhouse processing ca. 380 pigs/h between 2014-2015. sampling was performed during seven sessions. four carcass sites of 100 cm(2) each (back, belly, jowl externally, and the diaphragmatic area internally) were swabbed after evisceration. meat conveyors and dressing tables were tested swabbing areas of 200 to 400 cm(2). after pre-enrichment in buffered peptone water, samples were tested by sal ... | 2016 | 27800446 |
| the unrecognized burden of typhoid fever. | typhoid fever (tf), caused by salmonella enterica serovar typhi, is the most common cause of enteric fever, responsible for an estimated 129,000 deaths and more than 11 million cases annually. although several reviews have provided global and regional tf disease burden estimates, major gaps in our understanding of tf epidemiology remain. areas covered: we provide an overview of the gaps in current estimates of tf disease burden and offer suggestions for addressing them, so that affected communit ... | 2017 | 27797598 |
| prevalence of virulence and antimicrobial resistance genes in <i>salmonella</i> spp. isolated from commercial chickens and human clinical isolates from south africa and brazil. | salmonellosis is a significant public health concern around the world. the injudicious use of antimicrobial agents in poultry production for treatment, growth promotion and prophylaxis has resulted in the emergence of drug resistant strains of salmonella. the current study was conducted to investigate the prevalence of virulence and antimicrobial resistance genes from salmonella isolated from south african and brazilian broiler chickens as well as human clinical isolates. out of a total of 200 c ... | 2016 | 27796114 |
| bloodstream infection due to ctx-m-15 and tem-1 extended spectrum β-lactamase-producing salmonella enterica serovar virchow st16. | a 57-year-old man presented with a high fever and diarrhea. a blood culture showed the presence of a group c nontyphoidal salmonella isolate. on salmonella serotyping, the isolate was salmonella enterica serovar virchow. its sequence type was determined to be st16 by sequence analysis of seven different housekeeping genes. the blactx-m group 1 and blatem genes were amplified by multiplex pcr assays for detecting esbl genes. sequences of both amplicons were respectively identical to ctx-m-15- and ... | 2016 | 27795467 |
| salmonella enterica serovar kentucky flagella are required for broiler skin adhesion and caco-2 cell invasion. | nontyphoidal salmonella strains are the main source of pathogenic bacterial contamination in the poultry industry. recently, salmonella enterica serovar kentucky has been recognized as the most prominent serovar on carcasses in poultry-processing plants. previous studies showed that flagella are one of the main factors that contribute to bacterial attachment to broiler skin. however, the precise role of flagella and the mechanism of attachment are unknown. there are two different flagellar subun ... | 2017 | 27793824 |
| salmonella enterica serovar gallinarum: addressing fundamental questions in bacteriology sixty years on from the 9r vaccine. | sixty years on from smith's seminal work on fowl typhoid vaccines, there is renewed interest in experimental avian salmonellosis and in particular the use of salmonella enterica serovar gallinarum as a tool to understand key features of bacterial evolution and host adaptation. in this short review we outline some of the recent advances in avian salmonellosis research that have coupled both the power of whole genome analysis and new tools to understand the host response to existing experimental i ... | 2017 | 27791403 |
| virulence associated genes-deleted salmonella montevideo is attenuated, highly immunogenic and confers protection against virulent challenge in chickens. | to construct a novel live vaccine against salmonella enterica serovar montevideo (sm) infection in chickens, two important bacterial regulatory genes, lon and cpxr, which are associated with invasion and virulence, were deleted from the wild type sm genome. attenuated strains, jol1625 (δlon), jol1597 (δcpxr), and jol1599 (δlonδcpxr) were thereby generated. observations with scanning electron microscopy suggested that jol1625 and jol1599 cells showed increased ruffled surface which may be related ... | 2016 | 27785128 |
| multiple-locus variable-number tandem repeat analysis (mlva) for genotyping of salmonella enterica subspecies enterica serotype infantis isolated from human sources. | salmonella is an important cause of food-borne infection worldwide. detection of outbreaks caused by salmonella spp. relies on suitable and robust methods for genotyping. little is known about the genetic diversity of the salmonella enterica subspecies enterica serotype infantis strains isolated from human sources in iran. in this study, 40 isolates of s. infantis, which were previously recovered from patients with gastroenteritis or diarrhea in tehran between years 2007 and 2009, were subjected ... | 2016 | 27765620 |
| antagonistic activity of intestinal lactobacteria from domestic fowl against clinical isolates of salmonella enterica. | strain parameters of salmonellas and lactobacteria affecting their antagonistic activity were studied using an original method. real-time pcr was applied for measuring the decrease in the concentration of field isolates of salmonellas isolated from chickens in the presence of field isolates of lactobacteria also isolated from the gastrointestinal tract of chickens and broiler chickens. dispersion analysis showed that the probability of suppression of salmonella growth by lactobacteria is determi ... | 2016 | 27783299 |
| typhoid fever in south africa in an endemic hiv setting. | typhoid fever remains an important disease in africa, associated with outbreaks and the emerging multidrug resistant salmonella enterica serotype typhi (salmonella typhi) haplotype, h58. this study describes the incidence of, and factors associated with mortality due to, typhoid fever in south africa, where hiv prevalence is high. | 2016 | 27780232 |
| diagnosis of imported ugandan typhoid fever based on local outbreak information: a case report. | re-emerging multidrug-resistant typhoid fever is becoming a worldwide threat, especially in east africa. at the beginning of 2015, an outbreak of typhoid fever started in the capital city of uganda, and 1940 suspected cases were reported by 5 march 2015. in this report, we describe a case of typhoid fever caused by a mdr strain with hiv infection and hemoglobin s-syndrome thalassemia in an ugandan from kampala city. it is essential to consider mdr strains of salmonella enterica serovar typhi inf ... | 2016 | 27297635 |
| antimicrobial effect of the tunisian nana variety punica granatum l. extracts against salmonella enterica (serovars kentucky and enteritidis) isolated from chicken meat and phenolic composition of its peel extract. | punica granatum l. is widely recognized for its potency against a broad spectrum of bacterial pathogens. the purpose of this study was to explore the inhibitory and the bactericidal activities of punica granatum against salmonella strains. the effect of extracts obtained from different parts (peels, seeds, juice and flowers) of pomegranate and using different solvents against salmonella enterica serovars kentucky and enteritidis isolated from chicken meat was thus investigated. salmonella strain ... | 2017 | 27776287 |
| interferon-γ in salmonella pathogenesis: new tricks for an old dog. | salmonella enterica is a facultative intracellular bacterium that is the leading cause of food borne illnesses in humans. the cytokine ifn-γ has well-established antibacterial properties against salmonella and other intracellular microbes, for example its capacity to activate macrophages, promote phagocytosis, and destroy phagocytosed microbes by free radical-driven toxification of phagosomes. but ifn-γ induces the expression of hundreds of uncharacterized genes, suggesting that this cytokine de ... | 2016 | 27773552 |
| ctx-m-14 production by a clinical isolate of the european clone of salmonella enterica 4,[5],12:i. | | 2016 | 27771596 |
| feral pigeons: a reservoir of zoonotic salmonella enteritidis strains? | salmonella enterica infections in pigeons are generally associated with pigeon-adapted strains of serovar typhimurium that are of little public health concern. here, we isolated salmonella enteritidis phage type 4 (pt4), an important human pathogen, from a population of feral pigeons in brussels, which was further characterized by pulsed-field gel electrophoresis. all pigeon isolates belonged to the same pulsotype, which has been present in belgian pigeons at least since 2001 and is associated w ... | 2016 | 27771054 |
| phenotyping and genetic characterization of salmonella enterica isolates from turkey revealing arise of different features specific to geography. | 192 food samples (commonly consumed 8 food types), 355 animal samples (animal feces of bovine, ovine, goat and chicken) and 50 samples from clinical human cases in sanliurfa city, turkey in a year were collected to determine the salmonella enterica subsp. enterica mosaic in turkey. 161 salmonella isolates represented 17 serotypes, 20 sequence types (sts) and 44 pfge patterns (pts). 3 serotypes, s. enteritidis, s. typhimurium and s. kentucky, were recovered from three different hosts. the highest ... | 2017 | 27768932 |
| urbanized white ibises (eudocimus albus) as carriers of salmonella enterica of significance to public health and wildlife. | worldwide, salmonella spp. is a significant cause of disease for both humans and wildlife, with wild birds adapted to urban environments having different opportunities for pathogen exposure, infection, and transmission compared to their natural conspecifics. food provisioning by people may influence these factors, especially when high-density mixed species flocks aggregate. white ibises (eudocimus albus), an iconic everglades species in decline in florida, are becoming increasingly common in urb ... | 2016 | 27768705 |
| isolation and plasmid characterization of carbapenemase (imp-4) producing salmonella enterica typhimurium from cats. | carbapenem-resistant enterobacteriaceae (cre) are a pressing public health issue due to limited therapeutic options to treat such infections. cres have been predominantly isolated from humans and environmental samples and they are rarely reported among companion animals. in this study we report on the isolation and plasmid characterization of carbapenemase (imp-4) producing salmonella enterica typhimurium from a companion animal. carbapenemase-producing s. enterica typhimurium carrying blaimp-4 ... | 2016 | 27767038 |
| molecular interaction and cellular location of reca and chew proteins in salmonella enterica during sos response and their implication in swarming. | in addition to its role in dna damage repair and recombination, the reca protein, through its interaction with chew, is involved in swarming motility, a form of flagella-dependent movement across surfaces. in order to better understand how sos response modulates swarming, in this work the location of reca and chew proteins within the swarming cells has been studied by using super-resolution microscopy. further, and after in silico docking studies, the specific reca and chew regions associated wi ... | 2016 | 27766091 |
| biofilm formation, phenotypic production of cellulose and gene expression in salmonella enterica decrease under anaerobic conditions. | salmonella enterica subsp. enterica is one of the main food-borne pathogens. this microorganism combines an aerobic life outside the host with an anaerobic life within the host. one of the main concerns related to s. enterica is biofilm formation and cellulose production. in this study, biofilm formation, morphotype, cellulose production and transcription of biofilm and quorum sensing-related genes of 11 s. enterica strains were tested under three different conditions: aerobiosis, microaerobiosi ... | 2016 | 27592071 |
| minimal processing of iceberg lettuce has no substantial influence on the survival, attachment and internalization of e. coli o157 and salmonella. | the influence of a selection of minimal processing techniques (sanitizing wash prior to packaging, modified atmosphere, storage conditions under light or in the dark) was investigated in relation to the survival of, attachment to and internalization of enteric pathogens in fresh produce. cut iceberg lettuce was chosen as a model for fresh produce, escherichia coli o157:h7 (e. coli o157) and salmonella enterica were chosen as pathogen models. care was taken to simulate industrial post-harvest pro ... | 2016 | 27591385 |
| identification of potential therapeutics to conquer drug resistance in salmonella typhimurium: drug repurposing strategy. | salmonella typhimurium is the main cause of gastrointestinal illness in humans, and treatment options are decreasing because drug-resistant strains have emerged. | 2016 | 27761807 |
| identification and characterization of a gene stp17 located on the linear plasmid pbssb1 as an enhanced gene of growth and motility in salmonella enterica serovar typhi. | the linear plasmid pbssb1 mediates the flagellar phase variation in h:z66 positive salmonella enterica serovar typhi (s. typhi). the gene named stp17 (s. typhi plasmid number 17 gene) is located on pbssb1 and encodes the protein stp17. the expression pattern at the protein-level and function of stp17 remains unknown. in this study, the recombinant protein stp17his6 was expressed, purified and used to prepare the polyclonal anti-stp17 antibody. we detected protein-level expression of stp17 in s. ... | 2016 | 27761429 |
| spatial dependence of dna damage in bacteria due to low-temperature plasma application as assessed at the single cell level. | low temperature plasmas (ltps) generate a cocktail of reactive nitrogen and oxygen species (rnos) with bactericidal activity. the rnos however are spatially unevenly distributed in the plasma. here we test the hypothesis that this distribution will affect the mechanisms underpinning plasma bactericidal activity focussing on the level of dna damage in situ. for the first time, a quantitative, single cell approach was applied to assess the level of dna damage in bacteria as a function of the radia ... | 2016 | 27759098 |
| prevalence and antimicrobial resistance of salmonella isolated from two pork processing plants in alberta, canada. | this study investigated the frequency of salmonella serovars on pig carcasses at various processing steps in two commercial pork processing plants in alberta, canada and characterized phenotypic and genotypic antimicrobial resistance (amr) and pfge patterns of the salmonella isolates. over a one year period, 1000 swab samples were collected from randomly selected pigs at two slaughter plants. sampling points were: carcass swabs after bleeding (csab), carcass swabs after de-hairing (csad, plant a ... | 2017 | 27750110 |
| salmonella enterica synthesizes 5,6-dimethylbenzimidazolyl-(dmb)-α-riboside. why some firmicutes do not require the canonical dmb activation system to synthesize adenosylcobalamin. | 5,6-dimethylbenzimidazolyl-(dmb)-α-ribotide [α-ribazole-5'-phosphate (α-rp)] is an intermediate in the biosynthesis of adenosylcobalamin (adocbl) in many prokaryotes. in such microbes, α-rp is synthesized by nicotinate mononucleotide (namn):dmb phosphoribosyltransferases (cobt in salmonella enterica), in a reaction that is considered to be the canonical step for the activation of the base of the nucleotide present in adenosylcobamides. some firmicutes lack cobt-type enzymes but have a two-protei ... | 2017 | 27748967 |
| outbreak of salmonella enteritidis linked to the consumption of frozen beefburgers received from a food bank and originating from poland: northern france, december 2014 to april 2015. | a prolonged outbreak of salmonella enterica serotype enteritidis occurred in northern france between december 2014 and april 2015. epidemiological investigations following the initial notification on 30 december 2014 of five cases of salmonellosis (two confirmed s. enteritidis) in young children residing in the somme department revealed that all cases frequented the same food bank a. further epidemiological, microbiological and food trace-back investigations indicated frozen beefburgers as the s ... | 2016 | 27748250 |
| ultrasound improves chemical reduction of natural contaminant microbiota and salmonella enterica subsp. enterica on strawberries. | new sanitization methods have been evaluated to improve food safety and food quality and to replace chlorine compounds. however, these new methods can lead to physicochemical and sensory changes in fruits and vegetables. the present study evaluated the effects of acetic acid, peracetic acid, and sodium dodecylbenzenesulfonate isolated or combined with 5min of ultrasound treatment (40khz, 500w) on strawberry quality over 9days of storage at 8°c. the strawberry natural contaminant microbiota (mold ... | 2017 | 27743521 |
| immune protection of chickens conferred by a vaccine consisting of attenuated strains of salmonella enteritidis, typhimurium and infantis. | the colonization of poultry with different salmonella enterica serovars poses an issue throughout the world. in this study we therefore tested the efficacy of a vaccine consisting of attenuated strains of salmonella enterica serovars enteritidis, typhimurium and infantis against challenge with the same serovars and with s. agona, dublin and hadar. we tested oral and aerosol administration of the vaccine, with or without co-administration of cecal microbiota from adult hens. the protective effect ... | 2016 | 27741950 |
| draft genome sequences of four salmonella enterica strains isolated from turkey-associated sources. | we report the draft genomes of four salmonella enterica isolates evaluated for the contribution of plasmids to virulence. strains se163a, se696a, and se710a carry plasmids demonstrated to facilitate plasmid-associated virulence, while se819 is less virulent and has been used as a recipient for conjugation experiments to assess plasmid-encoded virulence mechanisms. | 2016 | 27738037 |
| influence of salmonella enterica serovar pullorum pathogenicity island 2 on type iii secretion system effector gene expression in chicken macrophage hd11 cells. | salmonella pathogenicity island 2 (spi2) can encode type iii secretion system 2 (t3ss2) which plays an important role in systemic disease development through delivering different effector proteins into host cells. here, the influence of salmonella pullorum pathogenicity island 2 on t3ss2 effector gene expression was studied using qrt-pcr in chicken macrophage hd11 cells. our results showed that all the detected genes (including pseudogenes sifb, ssph2 and stec) can express in hd11 cells of s. pu ... | 2017 | 27735192 |
| zinc is required to ensure the expression of flagella and the ability to form biofilms in salmonella enterica sv typhimurium. | zinc is known to play a central role in bacterial physiology and pathogenesis. here, we report that the accumulation of flic, the structural subunit of salmonella phase 1 flagella, is sharply reduced in a znuabc salmonella enterica sv. typhimurium strain grown in zinc-poor media. consequently, this mutant strain lacks motility, unless it grows in zinc-replete environments. this phenotype is the consequence of a general downregulation of all the genes involved in the biosynthesis of flagella, sug ... | 2016 | 27730246 |
| salmonella enterica serovar typhi and gallbladder cancer: a case-control study and meta-analysis. | in chile, where gallbladder cancer (gbc) rates are high and typhoid fever was endemic until the 1990s, we evaluated the association between salmonella enterica serovar typhi (s. typhi) antibodies and gbc. we tested 39 gbc cases, 40 gallstone controls, and 39 population-based controls for s. typhi vi antibodies and performed culture and quantitative polymerase chain reaction for the subset with bile, gallstone, tissue, and stool samples available. we calculated gender and education-adjusted odds ... | 2016 | 27726295 |
| correction: zinc is required to ensure the expression of flagella and the ability to form biofilms in salmonella enterica sv typhimurium. | correction for 'zinc is required to ensure the expression of flagella and the ability to form biofilms in salmonella enterica sv typhimurium' by serena ammendola et al., metallomics, 2016, doi: 10.1039/c6mt00108d. | 2016 | 27714016 |
| modelling the impact of water activity and fat content of dry-cured ham on the reduction of salmonella enterica by high pressure processing. | this work aimed to quantify the impact of aw and fat content of dry-cured ham on the log reduction of salmonella enterica by high pressure (hp). dry-cured ham with adjusted aw (0.86-0.96) and fat content (10-50%) was inoculated with s. enterica and pressurised (347-852mpa, 5min/15°c), following a central composite design. polynomial regression indicated a significant impact of pressure and aw on s. enterica hp-lethality. by lowering aw a clear piezoprotection was observed. at low aw (0.88) the s ... | 2017 | 27710771 |
| an extended genotyping framework for salmonella enterica serovar typhi, the cause of human typhoid. | the population of salmonella enterica serovar typhi (s. typhi), the causative agent of typhoid fever, exhibits limited dna sequence variation, which complicates efforts to rationally discriminate individual isolates. here we utilize data from whole-genome sequences (wgs) of nearly 2,000 isolates sourced from over 60 countries to generate a robust genotyping scheme that is phylogenetically informative and compatible with a range of assays. these data show that, with the exception of the rapidly d ... | 2016 | 27703135 |
| occurrence of mcr-1 in colistin-resistant salmonella enterica isolates recovered from humans and animals in italy, 2012 to 2015. | | 2016 | 27697756 |
| genomic and evolutionary analysis of two salmonella enterica serovar kentucky sequence types isolated from bovine and poultry sources in north america. | salmonella enterica subsp. enterica serovar kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. a genomic analysis of 119 isolates collected in the united states from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. results of the analysis demonstrated that the majority of poultry and bovine-associated s. kentucky were sequence type (st) 152. several bovine-associated (n ... | 2016 | 27695032 |
| complete genome sequence of a lytic siphoviridae bacteriophage infecting several serovars of salmonella enterica. | the bacteriophage 100268_sal2 was isolated from water buffalo feces in southern italy, exhibiting lytic activity against several subspecies of salmonella enterica this bacteriophage belongs to the siphoviridae family and has a 125,114-bp double-stranded dna (ds-dna) genome containing 188 coding sequences (cdss). | 2016 | 27688334 |