| systemic erysipelothrix rhusiopathiae infection not associated with endocarditis highlighting bacteriological diagnosis difficulties case report and literature review. | erysipelothrix rhusiopathiae is mostly isolated in swine causing erysipelas. human invasive infections due to e. rhusiopathiae remain poorly described and interestingly bacteraemia associated with endocarditis are a source of ineffective empirical antibiotherapy. we report a case of sepsis without endocarditis due to e. rhusiopathiae and a review of the literature. | 2016 | 27112902 |
| erysipelothrix rhusiopathiae bacteremia without endocarditis: rapid identification from positive blood culture by maldi-tof mass spectrometry. a case report and literature review. | erysipelothrix rhusiopathiae is a gram-positive bacillus that is infrequently responsible for infections in humans. three forms have been classified: a localized cutaneous form (erysipeloid) caused by traumatic penetration of e. rhusiopathiae, a generalized cutaneous form and a septicemic form. the latter type of disease has been previously associated with a high incidence of endocarditis. here we report a case of e. rhusiopathiae bacteremia in a 74-year-old man, probably started from an erysipe ... | 2016 | 27103974 |
| effects of amoxicillin, ceftiofur, doxycycline, tiamulin and tulathromycin on pig humoral immune responses induced by erysipelas vaccination. | it addition to their antimicrobial properties, antibiotics can influence the host immune system (modulation of cytokine secretion, antibody production and t-cell proliferation). in the present study, the authors studied the effects of therapeutic doses of amoxicillin (amx), ceftiofur (cef), doxycycline (doxy), tiamulin (tiam) and tulathromycin (tul) on the postvaccinal immune response after pigs had been vaccinated against erysipelas. because humoral immunity is considered as the most important ... | 2016 | 27072375 |
| reproduction of post-weaning multi-systemic wasting syndrome in an animal disease model as a tool for vaccine testing under controlled conditions. | snatch farrowed, colostrum deprived piglets were inoculated with different combinations of porcine circovirus 2, porcine parvovirus and erysipelothrix rhusiopathiae candidate vaccines. 10 piglets were mock-vaccinated. following virus challenge with a combined porcine circovirus 2/porcine parvovirus inoculum, all animals were monitored and samples taken for serology, immunohistochemistry and qpcr. at 24 dpc all non-vaccinated animals remaining were exhibiting signs of post-weaning multi-systemic ... | 2016 | 27033924 |
| virulence determinants, antimicrobial susceptibility, and molecular profiles of erysipelothrix rhusiopathiae strains isolated from china. | the aim of this study was to understand the epidemiology, serotype, antibiotic sensitivity, and clonal structure of erysipelothrix rhusiopathiae strains in china. forty-eight strains were collected from seven provinces during the period from 2012 to 2013. pulse-field electrophoresis identified 32 different patterns which were classified into clonal groups a–d. most pulsed-field gel electrophoresis (pfge) patterns were observed in clonal complex a and b, suggesting high diversity of genetic chara ... | 2015 | 26975059 |
| human erysipelothrix rhusiopathiae infection: unsolved issues and possible solutions. | erysipelothrix rhusiopathiae infection in humans may not be as rare as previously thought. in most cases, the disease is acquired from animals through work-related exposure. human infection has been reported since the early 1900's up to the present. unsolved issues associated with this organism include inadequate disease control in animals, difficulty in identification and isolation of the bacteria, diagnostic delay due to unawareness of this uncommon disease or unfamiliarity with the increasing ... | 2015 | 26817228 |
| fluorescent microbead-based immunoassay for anti-erysipelothrix rhusiopathiae antibody detection in cetaceans. | a fluorescent microbead-based immunoassay (fmia) for detection of anti-erysipelothrix rhusiopathiae antibodies in pigs was adapted for use in cetaceans. the fmia was validated and adjusted using serum samples from 10 vaccinated captive bottlenose dolphins tursiops truncatus collected between 1 and 13 mo after immunization. the technique was then used to analyze specimens from 15 free-ranging cetaceans stranded alive on the valencian mediterranean coast between 2006 and 2014: 11 striped dolphins ... | 2016 | 26758657 |
| unusual substrate specificity of the peptidoglycan mure ligase from erysipelothrix rhusiopathiae. | erysipelothrix rhusiopathiae is a gram-positive bacterium pathogenic to many species of birds and mammals, including humans. the main feature of its peptidoglycan is the presence of l-alanine at position 3 of the peptide stem. in the present work, we cloned the mure gene from e. rhusiopathiae and purified the corresponding protein as his6-tagged form. enzymatic assays showed that e. rhusiopathiae mure was indeed an l-alanine-adding enzyme. surprisingly, it was also able, although to a lesser ext ... | 2016 | 26700151 |
| tenosynovitis of a digit due to erysipelothrix rhusiopathiae: case report and review of the literature. | erysipelothrix rhusiopathiae is a gram-positive bacterium that in humans causes skin infections, such as erysipeloid, as a result of direct contact with contaminated animals or their waste or products. we present the first reported case of tenosynovitis of a finger in a 30-year-old woman. | 2015 | 26693283 |
| erysipelas outbreaks in flocks of geese in poland--biochemical and genetic analyses of the isolates. | erysipelothrix rhusiopathiae causes erysipelas in many vertebrate species. severe disease outbreaks have been noted in many poultry species--chickens, ducks, emus, pheasants, pigeons, and geese. this article describes the biochemical and genetic analyses of six e. rhusiopathiae strains isolated from geese for meat production. the isolates came from birds kept in different poultry houses on one farm, and were collected during two erysipelas outbreaks. we analyzed and compared the isolates by rand ... | 2015 | 26478164 |
| fatal erysipelothrix rhusiopathiae septicemia in two atlantic dolphins (stenella frontalis and tursiops truncatus). | we describe gross, histopathologic, ultrastructural, immunohistochemical, and microbiologic features of acute septicemia by erysipelothrix rhusiopathiae in an atlantic spotted dolphin stenella frontalis and an atlantic bottlenose dolphin tursiops truncatus. generalized lymphadenomegaly and widespread hemorrhages were the most consistent macroscopic findings. tricavitary effusion and icterus were noted in one individual. histologically, all organs examined showed numerous variably sized bacillary ... | 2015 | 26378410 |
| a combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of erysipelothrix rhusiopathiae strains from poultry and mammals. | erysipelothrix rhusiopathiae infections re-emerged as a matter of great concern particularly in the poultry industry. in contrast to porcine isolates, molecular epidemiological traits of avian e. rhusiopathiae isolates are less well known. thus, we aimed to (i) develop a multilocus sequence typing (mlst) scheme for e. rhusiopathiae, (ii) study the congruence of strain grouping based on pulsed-field gel electrophoresis (pfge) and mlst, (iii) determine the diversity of the dominant immunogenic pro ... | 2015 | 26198736 |
| development of an snp-based pcr assay for rapid differentiation of a japanese live vaccine strain from field isolates of erysipelothrix rhusiopathiae. | the differentiation of vaccine from non-vaccine isolates is important for disease control. based on single nucleotide polymorphisms identified by comparison of the genomes of the koganei 65-0.15 vaccine strain and a reference strain of erysipelothrix rhusiopathiae, we developed a pcr assay that can differentiate the vaccine strain from field isolates. | 2015 | 26151408 |
| safety and efficacy of a new octavalent combined erysipelas, parvo and leptospira vaccine in gilts against leptospira interrogans serovar pomona associated disease and foetal death. | the safety and protective efficacy of a new octavalent combination vaccine containing inactivated erysipelothrix rhusiopathiae, parvovirus, and leptospira interrogans (sensu lato) serogroups canicola, icterohaemorrhagiae, australis (bratislava), grippotyphosa, pomona and tarassovi - porcilis(®) ery+parvo+lepto - was evaluated in laboratory studies and under field conditions. the safety (2× overdose and repeated dose) was tested in 26 gilts. in this study, neither vaccine related temperature incr ... | 2015 | 26100922 |
| erysipelothrix rhusiopathiae associated with recent widespread muskox mortalities in the canadian arctic. | | 2015 | 26028673 |
| sepsis due to erysipelothrix rhusiopathiae in a patient with chronic lymphocytic leukemia associated with bronchopneumonia due to pseudomonas aeruginosa and escherichia coli: a case report. | the present report describes a case of sepsis due to erysipelothrix rhusiopathiae in a patient with b-cell chronic lymphocytic leukemia with no animal exposure, associated with concomitant bronchopneumonia due to pseudomonas aeruginosa and escherichia coli. | 2017 | 26015797 |
| erysipelothrix rhusiopathiae-induced aortic valve endocarditis: case report and literature review. | erysipelothrix rhusiopathiae is a pathogen of zoonosis often associated with occupational exposure. although erysipelothrix rhusiopathiae infection has high mortality, the heart valves in humans are rarely involved. the clinical data of a case of a 65-year-old male with erysipelothrix rhusiopathiae-induced aortic valve endocarditis was summarized retrospectively and analyzed with a literature review. based on a literature review and our experience, cases of e. rhusiopathiae-induced aortic valve ... | 2015 | 25785050 |
| presence and new genetic environment of pleuromutilin-lincosamide-streptogramin a resistance gene lsa(e) in erysipelothrix rhusiopathiae of swine origin. | erysipelothrix rhusiopathiae is a gram-positive bacillus that causes erysipelas in swine. in recent years, erysipelas infection among swine in china has been increasing. a combined resistance phenotype to pleuromutilins, lincosamides, and streptogramin a (plsa phenotype) was found in some e. rhusiopathiae isolates. the aim of this study was to identify the resistance genes responsible for the plsa phenotype in e. rhusiopathiae strains and to map the genetic environment of the identified resistan ... | 2015 | 25759293 |
| first report of macrolide resistance gene erm(t) harbored by a novel small plasmid from erysipelothrix rhusiopathiae. | the macrolide resistance gene erm(t) was identified for the first time in a porcine erysipelothrix rhusiopathiae isolate from swine in china. the novel 3,749-bp small plasmid per29, which carries erm(t), had a g+c content of 31% and four distinct open reading frames. the presence of per29 increased by at least 128-fold the mics of clindamycin and erythromycin for e. rhusiopathiae. the fitness cost of per29 could be responsible for the low frequency of erm(t) in e. rhusiopathiae. | 2015 | 25666150 |
| molecular detection of avian pathogens in poultry red mite (dermanyssus gallinae) collected in chicken farms. | poultry red mite (prm, dermanyssus gallinae) is a blood-sucking ectoparasite as well as a possible vector of several avian pathogens. in this study, to define the role of prm in the prevalence of avian infectious agents, we used polymerase chain reaction (pcr) to check for the presence of seven pathogens: avipox virus (apv), fowl adenovirus (fadv), marek's disease virus (mdv), erysipelothrix rhusiopathiae (er), salmonella enterica (se), mycoplasma synoviae (ms) and mycoplasma gallisepticum (mg). ... | 2014 | 25649939 |
| characterization of erysipelothrix rhusiopathiae strains isolated from acute swine erysipelas outbreaks in eastern china. | recently, a series of acute swine erysipelas outbreaks occurred in eastern china. eight strains isolated from cases of septicemia were determined as serotype 1a, and 4 of the isolates were resistant to acriflavine. one isolate strain named hx130709 was attenuated on agar media containing acriflavine dye. the 432-bp hypervariable region in spaa gene of the field and attenuated strains were amplified and sequenced. it was further compared with the vaccine strain g4t10, and thus, the eight field st ... | 2015 | 25649849 |
| erysipelothrix rhusiopathiae and mycoplasma hyopneumoniae: the sensitivities of enzyme-linked immunosorbent assays for detecting vaccinated sows of unknown disease status using serum and colostrum, and the correlation of the results for sow serum, colostrum, and piglet serum. | due to relatively high concentrations of immunoglobulins, colostrum has the potential to improve the sensitivity of diagnostic tests for diseases in pigs when compared with serum. it is possible that colostrum could improve the sensitivity of the antibody enzyme-linked immunosorbent assay (elisa) compared with serum. colostrum is also essential for piglets, providing protection against infections in the first few weeks and months of life. the sensitivity of 2 commercially available elisas, one f ... | 2015 | 25613041 |
| first case of osteomyelitis due to erysipelothrix rhusiopathiae: pubic osteomyelitis in a gored farmer. | we report the first proven case of osteomyelitis due to erysipelothrix rhusiopathiae. this infection occurred almost 20 years after traumatic inoculation of the bacterium, when the patient was gored by one of his cows. diagnosis was made by bone biopsies, and treatment included rifampicin and levofloxacin for 3 months. | 2015 | 25462176 |
| acute and chronic erysipelothrix rhusiopathiae infection in lambs. | polyarthritis caused by erysipelothrix rhusiopathiae is a relatively common infection in lambs characterized by low mortality and high morbidity. e. rhusiopathiae is a ubiquitous gram-positive bacterium that is both a commensal and a pathogen of vertebrates. the disease was studied during an outbreak in a norwegian spæl sheep flock. in the acute phase, 48 of 230 (20%) lambs developed clinical signs and 4 died (1.7%). one acute case was necropsied and e. rhusiopathiae was cultured from all major ... | 2015 | 25377692 |
| role of surface protective antigen a in the pathogenesis of erysipelothrix rhusiopathiae strain c43065. | to clarify the role of surface protective antigen a (spaa) in the pathogenesis of erysipelothrix rhusiopathiae c43065 (serotype 2), the spaa deletion mutant of e. rhusiopathiae δspaa was constructed by homologous recombination. the virulence of the δspaa mutant decreased more than 76-fold compared with that of the wild-type strain c43065 in mice. the mutant strain was sensitive to the bactericidal action of swine serum, whereas the wild-type strain was resistant. the adhesion of wild-type strain ... | 2015 | 25223326 |
| antibacterial activity of the essential oils of myrtle leaves against erysipelothrix rhusiopathiae. | to evaluate the antibacterial activity of the essential oil of myrtus communis (m. communis) l. against erysipelothrix rhusiopathiae (e. rhusiopathiae) in vitro. | 2014 | 25183140 |
| first report of erysipelothrix rhusiopathiae-associated septicaemia and histologic changes in cultured australian eels, anguilla reinhardtii (steindachner, 1867) and a. australis (richardson, 1841). | | 2015 | 25080974 |
| development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against erysipelothrix rhusiopathiae in captive bottlenose dolphins (tursiops truncatus). | erysipelothrix rhusiopathiae is the causative agent of erysipelas, a disease of many mammalian and avian species, mainly swine and turkeys. in cetaceans, erysipelas is considered to be the most common infection in juvenile individuals, which have not been vaccinated. moreover, the disease manifest in both forms, the dermatologic and the acute septicemic forms, has been reported in various species of dolphins and whales. it is difficult to diagnose erysipelas by currently available approaches. mo ... | 2014 | 25064337 |
| complete genome assembly and characterization of an outbreak strain of the causative agent of swine erysipelas--erysipelothrix rhusiopathiae sy1027. | erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and, to a fewer occurrences, human erysipeloid. it is ubiquitous in nature and commensal in diverse species of animals, wild or domestic, from mammals and birds to reptiles and fish. mechanisms of its virulence and pathogenicity are poorly understood. | 2014 | 24993343 |
| erysipelas in a free-range layer flock with conjunctival oedema as an unusual clinical sign. | erysipelas was diagnosed in a free-range laying flock with a high mortality of up to 7% per day and a severe decrease in egg production to 45%. the disease had a short course and unusual clinical features for erysipelas, including swollen, lacrimating and encrusted eyes. bacteriologically, trapped poultry red mites and affected animals were culture-positive for erysipelothrix rhusiopathiae. isolates from layers and mites were both serotype 1b. histopathology revealed disseminated intravasal coag ... | 2014 | 24881267 |
| phosphorylcholine and spaa, a choline-binding protein, are involved in the adherence of erysipelothrix rhusiopathiae to porcine endothelial cells, but this adherence is not mediated by the paf receptor. | a crucial event in the initiation of many bacterial infections is the adherence of the bacteria to host cells, and bacterial surface structures and their interactions with host cell receptors play an important role in this process. erysipelothrix rhusiopathiae is the causative agent of swine erysipelas, which may cause acute septicemia or chronic endocarditis and polyarthritis. to study the pathogenic mechanism of the widespread vascular disease observed in the acute form of swine erysipelas, we ... | 2014 | 24856134 |
| erysipelothrix rhusiopathiae bacteremia without endocarditis associated with psoas abscess: the first case report in thailand. | the authors report a patient with a rare manifestation of invasive septic erysipelothrix rhusiopathiae infection without endocardial involvement. our patient presented with progressive paraparesis and subacute fever for ten days. he had underlying diabetes mellitus and alcoholic cirrhosis. magnetic resonance imaging (mri) of the lumbosacral spine showed a psoas abscess with vertebral osteomyelitis and discitis at level of l23 of the lumbar spine. his blood culture grew e. rhusiopathiae. transtho ... | 2014 | 24772603 |
| erysipelothrix rhusiopathiae contamination in the poultry house environment during erysipelas outbreaks in organic laying hen flocks. | this study investigated organic laying hen farms for the presence of erysipelothrix rhusiopathiae in the house environment and from potential carriers (i.e. insects and mice) during ongoing erysipelas outbreaks, and compared the obtained isolates with those from laying hens. the samples were investigated by selective culture followed by species-specific polymerase chain reaction on cultures. e. rhusiopathiae was isolated from the spleen, jejunal contents, manure, dust and swabs from water nipple ... | 2014 | 24661145 |
| clinicoimmunopathologic findings in atlantic bottlenose dolphins tursiops truncatus with positive chlamydiaceae antibody titers. | sera from free-ranging atlantic bottlenose dolphins tursiops truncatus inhabiting the indian river lagoon (irl), florida, and coastal waters of charleston (chs), south carolina, usa, were tested for antibodies to chlamydiaceae as part of a multidisciplinary study of individual and population health. a suite of clinicoimmunopathologic variables was evaluated in chlamydiaceae-seropositive dolphins (n = 43) and seronegative healthy dolphins (n = 83). fibrinogen, lactate dehydrogenase, amylase, and ... | 2014 | 24492056 |
| prevalence of met-203 type spaa variant in erysipelothrix rhusiopathiae isolates and the efficacy of swine erysipelas vaccines in japan. | since 2009, erysipelas infection among pigs in japan has been increasing. this study investigated the prevalence, and characteristics of erysipelothrix rhusiopathiae isolates in japan from 2008 to 2010 and assessed the efficacy of current commercial erysipelas vaccines. based on polymorphisms in a 432-bp hypervariable region in the surface protective antigen a (spaa) gene, 34 isolates were classified into three groups: (i) group 1 with methionine at position 203 (met-203) and isoleucine at posit ... | 2014 | 24405986 |
| development of a loop-mediated isothermal amplification assay for rapid and simple detection of erysipelothrix rhusiopathiae. | erysipelothrix rhusiopathiae is a causative agent of swine erysipelas. we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for sensitive and rapid detection of e. rhusiopathiae. the lamp assay correctly detected 39 e. rhusiopathiae strains. no lamp products were detected from 14 non-rhusiopathiae erysipelothrix and 16 non-erysipelothrix strains, including e. tonsillarum serovar 10 strains, which are difficult to be discriminated from e. rhusiopathiae stra ... | 2014 | 24261887 |
| native valve endocarditis caused by erysipelothrix rhusiopathiae in an immunocompetent individual. | infective endocarditis is a very rare clinical form caused by erysipelothrix rhusiopathiae. it is rarely seen in immunocompetent individuals. even after surgery it may entail mortality rates as high as 30-40 %. this report describes a case of native valve endocarditis caused by e. rhusiopathiae and cured with crystallized penicillin g and surgery. | 2013 | 24072768 |
| prevalence of igy antibodies against erysipelothrix rhusiopathiae in a critically endangered parrot (kakapo, strigops habroptilus) and associated responses to vaccination. | an enzyme-linked immunosorbent assay (elisa) was developed to estimate levels of igy antibody against the bacterium erysipelothrix rhusiopathiae in serum samples collected from the critically endangered kakapo (strigops habroptilus, psittaciformes, aves) before and after vaccination against this bacterium. relative igy antibody titres in pre-vaccination serum samples (n = 71 individual kakapo) were normally distributed with the exception of four outliers which displayed low igy levels. notably a ... | 2013 | 24066897 |
| characterization and identification of a novel candidate vaccine protein through systematic analysis of extracellular proteins of erysipelothrix rhusiopathiae. | erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, is a facultative intracellular gram-positive bacterium. it has been shown that animals immunized with a filtrate from e. rhusiopathiae cultures are protected against lethal challenge. in this study, we identified and characterized the extracellular proteins of e. rhusiopathiae to search for novel vaccine antigens. a concentrated culture supernatant from the e. rhusiopathiae fujisawa strain, which has been found to induce prot ... | 2013 | 24019408 |
| erysipelothrix bacteremia without endocarditis: rare event or under-reported occurrence? | a patient presented with inflamed hands and erysipelothrix rhusiopathiae bacteremia. because a high incidence of endocarditis has been reported with this organism, a transesophageal echocardiogram was obtained, which was normal. treatment with oral moxifloxacin resolved all manifestations of illness. the association between e. rhusiopathiae bacteremia and endocarditis may be spurious. | 2013 | 23988830 |
| non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in re. coli fed-batch cultures. | in spite of the large number of reports on fed-batch cultivation of e. coli, alternative cultivation/induction strategies remain to be more deeply exploited. among these strategies, it could be mentioned the use of complex media with combination of different carbon sources, novel induction procedures and feed flow rate control matching the actual cell growth rate. here, four different carbon source combinations (glucose, glycerol, glucose + glycerol and auto-induction) in batch media formulation ... | 2013 | 23961396 |
| nonspecific erysipelothrix rhusiopathiae bacteremia in a patient with subclinical alcoholic liver disease. | erysipelothrix rhusiopathiae, a pleomorphic gram-positive bacillus, is found widely in nature or as a commensal pathogen. it infects domestic animals such as swine, which may be the major reservoir of the organism. e. rhusiopathiae is primarily an occupational illness; 89% of the cases are linked to high-risk epidemiological situations. humans that are infected by this bacillus typically present with one or a combination of the following symptoms: localized skin lesion (erysipeloid), diffuse cut ... | 2013 | 23819078 |
| septicemia and aortic valve endocarditis due to erysipelothrix rhusiopathiae in a homeless man. | we report a case of bacterial endocarditis due to erysipelothrix rhusiopathiae in a homeless man with no animal exposure. his course was complicated by an allergic reaction to ampicillin, urinary bladder infection, respiratory failure, and acute kidney injury. he recovered completely after aortic valve replacement and a 6-week course of intravenous ceftriaxone. | 2013 | 23662222 |
| erysipelothrix rhusiopathiae exploits cytokeratin 18-positive epithelial cells of porcine tonsillar crypts as an invasion gateway. | tonsils are important organs for mucosal immunity and are gateways for various pathogens, including bacteria and viruses. the purpose of the present study was to reveal how erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, invades the mucosal epithelium of the tonsils of pigs. two germ-free piglets were orally infected with e. rhusiopathiae koganei 65-0.15, an attenuated vaccine strain in japan, and their tonsils of the soft palate were histologically examined four weeks aft ... | 2013 | 23601839 |
| something fishy: an unusual erysipelothrix rhusiopathiae infection in an immunocompromised individual. | a 39-year-old man with a history of crohn's disease presented to the emergency department with a 12-h history of worsening febrile illness. he deteriorated rapidly with progression to sepsis and was admitted to the intensive care unit. initial treatment comprised broad spectrum antibiotics and inotropic support resulting in rapid improvement. with the immunocompromised state of the patient, and multiple blood cultures revealing erysipelothrix rhusiopathiae, a detailed history disclosed a recent ... | 2013 | 23559657 |
| mitral regurgitation jet around neoannulus: mitral valve replacement in erysipelothrix rhusiopathiae endocarditis. | a 50-year-old male presented with erysipelothrix rhusiopathiae (er) endocarditis of the mitral valve, severe mitral regurgitation, and heart failure. the er endocarditis destroyed the native mitral annulus therefore a new annulus was created for the suspension of the mitral bioprosthesis. postoperative neoannulus dehiscence and leak prompted to redo surgery where transesophageal echocardiography (tee) played an important role in pointing out the exact location of perineoannular leaks for repair. | 2016 | 23545868 |
| erysipelas in laying hens is associated with housing system. | following the change from conventional cages to non-cage housing systems and furnished cages, which in sweden was finalised by 2005, problems caused by erysipelothrix rhusiopathiae increased in laying hen flocks. this study aimed to investigate possible associations between housing systems for laying hens and outbreaks of erysipelas. also, sera from 129 flocks in different housing systems, collected during 2005-2007, were analysed for the presence of antibodies to e rhusiopathiae using an indire ... | 2013 | 23542656 |
| erysipelas in a numbat (myrmecobius fasciatus). | an adult, female numbat (myrmecobius fasciatus) was submitted to the perth zoo veterinary department for postmortem examination in november 2011. this radio-collared wild numbat had been found dead in the dryandra woodland, 191 km southeast of perth, western australia. on external examination, the body condition was good. three ticks (ixodes spp.) were found on the thoracic region. the external pouch was contaminated with dirt and palpably flocculent, and the nipples oozed a purulent material. h ... | 2013 | 23505731 |
| improving ante mortem diagnosis of erysipelothrix rhusiopathiae infection by use of oral fluids for bacterial, nucleic acid, and antibody detection. | swine erysipelas is an economically important disease caused by erysipelothrix rhusiopathiae. pen-based collection of oral fluids has recently been utilized for monitoring infection dynamics in swine operations. the diagnostic performance of bacterial isolation, real-time pcr, and antibody detection by enzyme-linked immunosorbent assay (elisa) and fluorescent microbead-based immunoassay (fmia) methods were evaluated on pen-based oral fluid samples from pigs experimentally infected with e. rhusio ... | 2013 | 23201482 |
| a case of apparent canine erysipeloid associated with erysipelothrix rhusiopathiae bacteraemia. | erysipelothrix rhusiopathiae is a gram-positive facultative anaerobe found worldwide and is most commonly associated with skin disease in swine, while anecdotal reports of cases in dogs have been associated with endocarditis. | 2012 | 23140319 |
| erysipelothrix rhusiopathiae isolates recovered from fish, a harbour seal (phoca vitulina) and the marine environment are capable of inducing characteristic cutaneous lesions in pigs. | in order to determine the diversity and pathogenicity of erysipelothrix spp. isolates recovered from marine fish, a harbour seal (phoca vitulina) and the marine environment, 14 isolates were characterized by genotyping, serotyping, determination of the surface protective antigen (spa) gene type and assessment of virulence in a pig bioassay. all 14 isolates were erysipelothrix rhusiopathiae. isolates were determined to be of serotypes 2 (n = 3), 3 (n = 1), 4 (n = 1), 12 (n = 1), 15 (n = 1) or 21 ... | 2013 | 23083834 |
| cutaneous erysipelothrix rhusiopathiae (erysipeloid) infection in an immunocompromised child. | erysipeloid, a cutaneous infection with the gram-positive bacillus erysipelothrix rhusiopathiae, is typically an occupational dermatosis seen in persons working with livestock or involved in commercial fishing (fishmongers). other more-generalized forms of infection with this organism also exist, including a septic form usually associated with endocarditis. many infections may be self-limited. they have rarely been reported in children or in immunocompromised patients. this microbe is sensitive ... | 2014 | 22957967 |
| capsular polysaccharide of erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, and its modification with phosphorylcholine. | the capsule has been implicated in the virulence of the swine pathogen erysipelothrix rhusiopathiae, a rod-shaped, intracellular gram-positive bacterium that has a unique phylogenetic position in the phylum firmicutes and is a close relative of mollicutes (mycoplasma species). in this study, we analyzed the genetic locus and composition of the capsular polysaccharide (cps) of the fujisawa strain of e. rhusiopathiae. genome analysis of the fujisawa strain revealed that the genetic locus for capsu ... | 2012 | 22949554 |
| development of a novel fluorescent microbead-based immunoassay and comparison with three enzyme-linked immunoassays for detection of anti-erysipelothrix spp. igg antibodies in pigs with known and unknown exposure. | a novel fluorescent microbead immunoassay (fmia) using the recombinant polypeptide spaa415 was developed for detection of anti-erysipelothrix spp. igg in pig sera. the diagnostic performance of the fmia was evaluated on samples from pigs with known and unknown erysipelothrix spp. exposure and compared to an in-house enzyme-linked immunosorbent assay (elisa-1) based on the same capture antigen, and two commercially available elisas (elisa-2 and elisa-3). sera from pigs experimentally infected wit ... | 2012 | 22820200 |
| immunostimulatory effects of recombinant erysipelothrix rhusiopathiae expressing porcine interleukin-18 in mice and pigs. | interleukin-18 (il-18), which was originally called gamma interferon (ifn-γ)-inducing factor, has been shown to play an important role in innate and acquired immune responses. in this study, attenuated erysipelothrix rhusiopathiae strains were engineered to produce porcine il-18 (poil-18) and evaluated for their potential immunostimulatory effect in animals. recombinant poil-18 was successfully expressed in the recombinant e. rhusiopathiae strains ys-1/il-18 and ko/il-18. the culture supernatant ... | 2012 | 22761300 |
| cloning, auto-induction expression, and purification of rspaa swine erysipelas antigen. | this work reports the cloning, expression, and purification of a 42-kda fragment of the spaa protein from erysipelothrix rhusiopathiae, the main antigenic candidate for a subunit vaccine against swine erysipelas. the use of an auto-induction protocol to improve heterologous protein expression in recombinant escherichia coli cultures was also investigated. the cellular growth pattern and metabolite formation were evaluated under different induction conditions. the his-tagged protein was over-expr ... | 2012 | 22729211 |
| active infective endocarditis due to erysipelothrix rhusiopathiae: zoonosis caused by vancomycin-resistant gram-positive rod. | a 42-year-old female who was a voluntary worker in a school for handicapped children was referred to us for surgery for active infective endocarditis. trans-esophageal echocardiography showed 2 large mobile vegetations on the aortic valve and severe aortic regurgitation. aortic valve replacement was performed to prevent septic embolism and deterioration of congestive heart failure. the empiric therapy with vancomycin, ampicillin, and gentamycin was initiated because a pathogen was not identified ... | 2013 | 22664947 |
| collagen adhesin-nanoparticle interaction impairs adhesin's ligand binding mechanism. | pathogenic bacteria specifically recognize extracellular matrix (ecm) molecules of the host (e.g. collagen, fibrinogen and fibronectin) through their surface proteins known as mscramms (microbial surface components recognizing adhesive matrix molecules) and initiate colonization. on implantation, biomaterials easily get coated with these ecm molecules and the mscramms mediate bacterial adherence to biomaterials. with the rapid rise in antibiotic resistance, designing alternative strategies to re ... | 2012 | 22538248 |
| successful medical treatment of erysipelothrix rhusiopathiae-induced lumbosacral diskospondylitis in a dog. | | 2012 | 22505240 |
| effect of age and maternally-derived antibody status on humoral and cellular immune responses to vaccination of pigs against erysipelothrix rhusiopathiae. | the effects of age and maternally-derived antibodies (mda) on the immune response to erysipelothrix rhusiopathiae were investigated in piglets orally vaccinated with a live e. rhusiopathiae vaccine at 6, 8 or 10 weeks of age. seroconversion, determined by elisa, was evident in mda positive piglets vaccinated at 8 or 10 weeks of age and in all mda negative vaccinates. two weeks after vaccination in the presence of mda, a t cell response, measured by a lymphocyte proliferation assay, was observed ... | 2012 | 22498786 |
| characterization of erysipelothrix rhusiopathiae strains isolated from recent swine erysipelas outbreaks in japan. | the objective of the present study was to characterize erysipelothrix sp. strains from recent erysipelas outbreaks in japan. eighty-three (100%) strains were identified as e. rhusiopathiae, based on serotyping and spaa pcr. fifty (60.3%), 5 (6.0%), and 28 (33.7%) strains were isolated from animals with acute, subacute and chronic outbreaks, respectively, of which 79 (95.2%), 1 (1.2%), and 3 (3.6%) belonged to serotypes 1a, 2a, and untypeable, respectively. fifteen strains (including 3, 2, and 10 ... | 2012 | 22446396 |
| development of an enzyme-linked immunosorbent assay for the serological detection of exposure of poultry in new zealand to erysipelothrix rhusiopathiae and their serological response to vaccination. | to modify and validate an existing swine erysipelas elisa for use with poultry serum and to assess the safety of a swine erysipelas vaccine for use in new zealand layer birds. | 2012 | 22352927 |
| [preparation and property study of the recombinant camel anti-spaa-n single domain antibodies]. | in order to obtain single domain antibody against surface protective antigen a (spaa)of erysipelothrix rhusiopathiae. | 2012 | 22230504 |
| antimicrobial resistance in bacterial poultry pathogens: a review. | antimicrobial resistance (amr) is a global health threat, and antimicrobial usage and amr in animal production is one of its contributing sources. poultry is one of the most widespread types of meat consumed worldwide. poultry flocks are often raised under intensive conditions using large amounts of antimicrobials to prevent and to treat disease, as well as for growth promotion. antimicrobial resistant poultry pathogens may result in treatment failure, leading to economic losses, but also be a s ... | 2017 | 28848739 |
| microbiological and molecular identification of bacterial species isolated from nasal and oropharyngeal mucosa of fuel workers in riyadh, saudi arabia. | this study aimed to determine the bacterial species colonizing the nasal and oropharyngeal mucosa of fuel workers in central riyadh, saudi arabia on a microbiological and molecular level. throat and nasal swab samples were obtained from 29 fuel station attendants in the period of time extending from march to may 2014 in riyadh, saudi arabia. microbiological identification techniques were utilized to identify the bacterial species isolated. antibiotic sensitivity was assessed for each of the bact ... | 2017 | 28855823 |
| isolation and detection of erysipelothrix rhusiopathiae and its distribution in humans and animals by phenotypical and molecular methods in ahvaz-iran in 2015. | erysipelothrix rhusiopathiae (e. rhusiopathiae) is generally transmitted into the gastrointestinal tract of animals by the intake of contaminated food or water and causes great economic loss in agriculture worldwide. some of the erysipelothrix spp. are the causative agents of erysipeloid, which is an occupational infection in humans. the aim of the present study was to isolate e. rhusiopathiae from animals as well as the hands of the butchers working in ahvaz, iran, and to determine their suscep ... | 2017 | 28761204 |
| serotypes and spa types of erysipelothrix rhusiopathiae isolates from british pigs (1987 to 2015). | erysipelothrix spp. cause a range of clinical signs in pigs and at least 28 different erysipelothrix spp. serotypes have been identified. in this study, 128 isolates of erysipelothrix spp. from pigs in great britain from 1987 to 2015 were characterised by serotyping and multiplex real time pcr assays targeting the surface protective antigen (spa) and the main genotypes (erysipelothrix rhusiopathiae, erysipelothrix tonsillarum and erysipelothrix spp. strain 2). all 128 british isolates were chara ... | 2017 | 28720292 |
| multiplex pcr assay for the simultaneous detection and differentiation of clonal lineages of erysipelothrix rhusiopathiae serovar 1a strains currently circulating in japan. | the species erysipelothrixrhusiopathiae displays genetic heterogeneity; however, e. rhusiopathiae serovar 1a strains currently circulating in japan exhibit remarkably low levels of genetic diversity and group into clonal sublineages of lineage ivb (ivb-1 and ivb-2). in the present study, based on whole genome sequencing data, we designed primers for a multiplex pcr assay to simultaneously detect and differentiate the sublineages of e. rhusiopathiae strains. among the one hundred and twenty-seven ... | 2017 | 28637945 |
| a novel octavalent combined erysipelas, parvo and leptospira vaccine provides (cross) protection against infection following challenge of pigs with 9 different leptospira interrogans serovars. | in the present study the efficacy and cross protection of a novel ready-to-use combination vaccine, porcilis® ery + parvo + lepto, against swine leptospirosis, was investigated. the octavalent vaccine contains inactivated antigens of erysipelothrix rhusiopathiae, parvovirus and 6 leptospira interrogans sensu lato strains of serogroups canicola, icterohaemorrhagiae, grippotyphosa, australis (bratislava), pomona and tarassovi. in this study ninety pigs were vaccinated twice with porcilis® ery + pa ... | 2015 | 28405422 |