| adequacy of breast-feeding. | there are 3 main errors in the paper by professor waterlow and dr. thomson on the subject of adequacy of breast feeding. the errors relate to the following: validity of recommended dietary allowances (rda), balance between intake and requirements, and frequency of weight faltering. far from being "generally accepted," the rdas for early infancy (0-6 months) are highly tenuous. as waterlow and thomson indicate, they are largely based on s.j. fomon's studies on intakes observed in north americ ... | 1979 | 90775 |
| increased tendency to spontaneous in-vitro lymphocyte transformation in clinically active multiple sclerosis. | lymphocytes from 8 of 10 patients with clinically active multiple sclerosis (ms) but from only 3 of 18 patients with quiescent ms and 4 of 20 healthy donors transformed spontaneously on in-vitro culture. the transformed cells from all donors had the characteristics of b lymphocytes (surface and intracytoplasmic immunoglobulin and complement receptors) and carried antigens of epstein-barr virus. it is suggested that these results are further evidence that immunoregulation in active ms is abnormal ... | 1979 | 90801 |
| bacteriological criteria for feeding raw breast-milk to babies on neonatal units. | aerobic cultures of 207 samples of drip breast-milk from seventy mothers in hospital showed that 6 (3%) were sterile and 170 (82%) contained only the commensal organisms coagulase-negative staphylococci and streptococcus viridans. 25 (15%) grew potential pathogens--staphylococcus aureus in 13 (6%); enterobacteria in 15 (7%); and group-b streptococci in 3 (2%). it is proposed that milk with commensals in any numbers may be considered for use unheated but not milk containing any potential pathogen ... | 1979 | 90814 |
| group-b streptococcal infection in the newborn. | | 1979 | 90847 |
| hyperammonaemia, plasma aminoacid imbalance, and blood-brain aminoacid transport: a unified theory of portal-systemic encephalopathy. | it is proposed that hyperammonaemia in liver cirrhosis or after portacaval shunt contributes to plasma neutral aminoacid imbalance and to increased activity of the blood-brain neutral amino-acid transport system. plasma neutral aminoacid concentrations are deranged, partly, but not completely, because ammonia stimulates glucagon secretion; a high rate of gluconeogenesis and hyperinsulinaemia follow. brain uptake of neutral aminoacids rises because ammonia stimulates brain-glutamine synthesis, wh ... | 1979 | 90864 |
| cyclosporin a. | | 1979 | 90866 |
| splenectomy and karyotypic conversion in chronic myeloid leukaemia. | | 1979 | 90884 |
| alpha 1-antitrypsin in human macrophages. | | 1979 | 91062 |
| [operative indication of large decompressive craniectomy for cerebral contusion with subdural hematoma (author's transl)]. | | 1979 | 91131 |
| transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. | we describe a rapid and very sensitive method for detecting proteins as antigens after their separation in polyacrylamide/agarose composite gels, with or without sodium dodecyl sulfate. the polyacrylamide matrix is crosslinked with a reagent that can be cleaved with periodate or alkali to facilitate transfer of the protein bands to diazobenzyloxymethyl-paper, where they are coupled covalently. specific proteins are detected by autoradiography after sequential incubation with unfractionated, unla ... | 1979 | 91164 |
| the structure and evolution of the hla--bw4 and bw6 antigens. | the hla--bw4 and bw6 antigenic determinants have been shown to co-migrate with hla--b determinants on gel-filtration, sucrose density gradient centrifugation and affinity chromatography, using lens culinaris lectin and antibody against human beta 2 microbulin. these and other published data imply that the hla--bw4 and bw6 determinants reside on the same polypeptide chain as other hla--b locus determinants. the implications of this in terms of the evolution of cross reacting groups of antigens at ... | 1979 | 91209 |
| monolayer absorption of human cytotoxic t cells: evidence for clonality. | | 1979 | 91211 |
| glutathione conjugate formation from hexachlorocyclohexane and pentachlorocyclohexene by rat liver in vitro. | | 1979 | 91272 |
| lymphocyte transformation in the presence of myelin basic protein in multiple sclerosis and control subjects. | the transformation of lymphocytes in vitro in the presence of human myelin basic protein has been investigated in normal people, patients with multiple sclerosis (ms) and controls with other neurological diseases. there was little or no response at low concentrations (1--10 microgram/ml) but significant transformation at higher concentrations (100--1000 microgram/ml) in all three groups. there was no significant difference among the groups as a whole, but those ms patients who had had disease fo ... | 1979 | 91302 |
| the development of mesosomes of staphylococcus aureus during autolysis. | mesosomes of staphylococcus aureus were examined morphologically under the electron microscope. three different methods of specimen preparation (thin section, freeze-fracture, and negative staining) were used to eliminate artifacts due to sample processing. mesosomes were rarely seen in intact cells but were quite distinct in autolysed cells incubated in 1.2m sucrose -0.33m acetate buffer (ph 5.8) at 25 degrees c. the phospholipid content increased by 20% of the control as autolysis proceeded. s ... | 1979 | 91308 |
| methyl xanthines, adenosine 3',5'-cyclic monophosphate and the spinal transmission of nociceptive information. | 1 in spinal cats anaesthetized with either alpha-chloralose or sodium pentobarbitone, a study was made of the effects of adenosine 3',5'-cyclic monophosphate (cyclic amp), mono- and di-butyryl cyclic amp and the methyl xanthines, theophylline and isobutyl methyl xanthine (ibmx), on the responses of neurones of laminae i, iv and v to noxious and innocuous skin stimuli. the compounds were administered from micropipettes positioned in the substantia gelatinosa. ibmx was also given intravenously.2 w ... | 1979 | 91402 |
| transport of vitamin d metabolites. | vitamin d and its metabolites are bound to an alpha globulin (dbp) in human serum. this carrier protein binds 25-ohd and 24,25(oh)2d with higher affinity than vitamin d or 1,25(oh)2d, but the binding is highly specific for the vitamin d structure. the carrier mechanism appears to be unique in that it is a high affinity and high capacity system, capable of binding as much as 120,000 iu of biological activity per liter of plasma. dbp is apparently identical to group-specific component, and a dbp-d ... | 1979 | 91467 |
| procaine-induced seizures in epileptic monkeys with bilateral hippocampal foci. | intravenous procaine hcl given at low doses (0.5-2.5 mg/kg) to two monkeys with bilateral alumina hippocampal foci depressed interictal spiking or had little effect. at 5.0 mg/kg unilateral limbic activation occurred. at 10.0 mg/kg unilateral or bilateral limbic activation and generalized seizures could be evoked within 3-10 min. at higher doses (15 and 20 mg/kg) bilateral limbic activation or brief (one min) generalized seizures occurred. the unilateral-onset psychomotor seizures were not ident ... | 1979 | 91502 |
| in vitro evidence from anti-hapten antibody responses for t helper and suppressor cells directed against major histocompatibility antigens in the mouse. participation of i region determinants in the induction of t helper cells. | | 1979 | 91525 |
| immunological specificity of natural opsonins and their role in the cross-reactivity between staphylococcus aureus mardi and escherichia coli 101. | the immunochemical specificity of the observed cross-reactivity between escherichia coli strain 101 and staphylococcus aureus strain mardi was examined. the cross-reactivity was shown to be dependent upon mucopeptide antibodies which are present in normal and immune sera. although both organisms contained surface antigens with immunodominant glucuronic acid residues, in vitro phagocytosis studies indicated that antibodies directed against these antigens were not significantly involved in the ops ... | 1979 | 91583 |
| a rat serum glycoprotein whose synthesis rate increases greatly during inflammation. | crossed immunoelectrophoresis of rat serum demonstrated considerably increased serum concentrations of at least ten different proteins during turpentine-induced inflammation. one protein, which moved during electrophoresis like an alpha 1 globulin, showed a particularly large increase. this protein was purified to homogeneity by ammonium sulfate fractionation followed by chromatography on deae-cellulose. sephadex g-100, and concanavalin a-sepharose, and finally disc electrophoresis in polyacryla ... | 1979 | 91605 |
| human endodermal sinus tumour in nude mice and its markers for diagnosis and management. | two human endodermal sinus tumours (yolk sac tumours) were transplanted successfully into nude mice. the transplanted tumours maintained not only morphological characters, such as schiller-duval bodies, but also the ability to synthesise alpha-fetoprotein, lactic dehydrogenase 1, liver and bone type alkaline phosphatase, and some human serum proteins. since these tumours produced lactic dehydrogenase 1 but not the other four isozymes of lactic dehydrogenase, this isozyme, like alpha-fetoprotein, ... | 1979 | 91627 |
| antigenic determinants of influenza virus hemagglutinin. iv. immunogenicity of fragments isolated from the hemagglutinin of a/memphis/72. | | 1979 | 91638 |
| binding of purified, soluble major histocompatibility complex polypeptide chains onto isolated t-cell receptors. i. reactivity against allo- and self-determinants. | in this study, we tried to get information about the fine antigen-binding ability of purified, soluble, idiotype-positive t-cell receptor molecules. lewis anti-da t-cell receptors were purified from normal lewis serum by the use of anti-idiotypic immunosorbent and sodium dodecyl sulfate-polyacrylamide gel, and were coupled to cyanogen bromide-activated sepharose 4b. in parallel, lewis anti-da, lewis anti-bn, and da anti-lewis alloantibody immunosorbents were prepared. the major ag-b chain (44,00 ... | 1979 | 91653 |
| variation in orbiviruses. | | 1979 | 91662 |
| sensitivity of the short-range spinal interneurons of the cat to experimental spinal cord trauma. | the technique of retrograde axoplasmic transport was used to demonstrate the effect of experimental spinal cord injury on the spinal interneurons in the upper lumbar and lower thoracic segments of cats. force of varied intensity was applied to the dorsal surface of the spinal cord and horseradish peroxidase (hrp) was injected into the next caudal segment. a large impact (250 to 350 gm-cm) inducing permanent paraplegia of the hind legs blocked the axoplasmic transport instantaneously in both cran ... | 1979 | 91677 |
| the declaration of alma-ata and the future of primary care. | | 1979 | 91728 |
| [enzymatic synthesis and characterization of dna complementary to ceruloplasmin mrna from rat liver]. | poly(a) containing rat liver 21s rna homogeneous in polyacrylamide gel electrophoresis under denaturing conditions and stimulating the synthesis of ceruloplasmin in a cell-free proteinsynthesizing system, was used as a template for reverse transcription in the presence of t10 primer and highly purified reverse transcriptase from avian myeloblastosis virus. the cdna made this way was characterized by means of hybridization kinetics with mrna, by melting of the hybrids formed and by chain length m ... | 1979 | 91944 |
| myosin isoenzyme redistribution in chronic heart overload. | since the first observation by spann et al., it has become clear that in cardiac hypertrophy induced by a mechanical overloading, the velocity of shortening of the cardiac muscle (vmax) is reduced (see ref. 2 for review). most authors agree that this mechanical alteration is accompanied by a decrease in the ca2+-dependent atpase activity of myosin (see ref. 3 for review). the molecular basis of such changes was unknown because the structural modifications of the myosin molecule were ill-defined. ... | 1979 | 91973 |
| [heterologous transplantation of the human medulloblastoma--with particular reference to the histogenesis of medulloblastoma (author's transl)]. | | 1979 | 91984 |
| [the effect of cerebral perfusion pressure in cerebral infarction after occlusion of middle cerebral artery in dog (author's transl)]. | | 1979 | 91991 |
| concanavalin a stimulates phospholipid methylation and phosphatidylserine decarboxylation in rat mast cells. | when histamine release from rat peritoneal mast cells is stimulated by concanavalin a, membrane phospholipids are methylated in the early stage of this process. exogenously added phosphatidylserine enhances the concanavalin a-induced histamine release, and at the same time the lectin markedly stimulates the decarboxylation and methylation of phosphatidylserine. within minutes after the addition of concanavalin a to rat mast cells, the newly methylated phospholipids begin to disappear and an incr ... | 1979 | 92021 |
| immunology of infective endocarditis. | | 1979 | 92039 |
| [influence of the pineal gland on the hypophyseal function of the rat in vivo (proceedings)]. | | 1979 | 92226 |
| inhibitory effect of tricyclic antidepressant drugs on spontaneous activity of noradrenergic neurons. relation with blood and brain concentrations [proceedings]. | | 1979 | 92235 |
| [natural inhibition of biotransformations of arachidonic acid (proceedings)]. | | 1979 | 92241 |
| failure of corticosteroids to influence three enzymes involved in neurotransmitter metabolism in adult rat brain [proceedings]. | | 1979 | 92255 |
| shielding of phospholipid vesicles from phospholipase c hydrolysis by alpha-lactalbumin adsorption [proceedings]. | | 1979 | 92261 |
| [hydrolysis of glucose 6-phosphate in bovine thyroid tissue (proceedings)]. | | 1979 | 92262 |
| occurrence of isoprene lipids in bovine thyroid [proceedings]. | | 1979 | 92282 |
| [experimental use of prostaglandin f2 alpha to synchronize estrus in cattle]. | experiments were conducted for the use of prostaglandin to synchronise oestrus in dairy cows. one single dose of 5 mg f2-alpha prostaglandin was administered via the intrauterine route. the reference values of the control groups were matched by first insemination following induced oestrus among the best of the test animals which were inseminated twice. these values reached 72 and 96 hours following application accounted for 46.7 and 50.0 per cent. the yellow body phase, a prerequisite for oestru ... | 1979 | 92295 |
| human adenohypophyseal quantitative histochemical cell classification. i. morphologic criteria and cell type distribution. | recent advances in immunocytochemistry and histochemical staining have made it possible to identify pituitary cells that contain specific hormones by their appearance under light microscopy. criteria for identification of functional cell classes were formulated from these studies. fourteen hypophyses were obtained at the time of autopsy (four to 22 hours postmortem), embedded in paraffin, sectioned, and differentially stained. cell counts on areas in the acidophil wing and basophil wedge were pe ... | 1979 | 92296 |
| ["non-specific cross reacting antigen (nca)", a tracer of human granulocytes and monocytes]. | the non specific cross reacting antigen, or nca, is a normal tissue antigen that cross reacts with cea. it bears a specific antigenic determinant that is absent from cea. immunocytological studies first pointed out that nca, but not cea, is present in alveolar macrophages and polymorphonuclears. further work demonstrated that nca is a marker of granulocytic series: it appears at the stage of promyelocyte and likely is linked to the azurophilic granules. the same antigen is also present in periph ... | 1979 | 92357 |
| [a comparison of the immunosuppressive effect of normal and purified alpha-globulin fraction of human plasma]. | | 1979 | 92373 |
| antiviral agents: action and clinical use. | the development of antiviral agents has been hindered by a variety of problems. there are fundamental biological differences between viruses and other infectious agents. viruses are strictly dependent on cellular metabolic processes and possess very limited intrinsic enzyme systems and building blocks which may serve as targets for drugs. antiviral drugs must also possess the ability to enter the host cell. viral replication consists of a series of events, each of which can be interfered with, l ... | 1979 | 92398 |
| synthesis of an immunogen with catechol estrogen as immunodeterminant group and its characterization by the induced antibodies. | | 1979 | 92446 |
| populational polymorphisms in silver staining of nucleolus organizer regions (nors) in human acrocentric chromosomes. | the ag stainability of the nucleolus organizer region (nor) was studied in the acrocentric chromosomes identified by q banding of cultured lymphocytes in 41 karyotypically normal persons (33 males and 8 females) originating from southeast estonia. the data obtained are compared with those established earlier for a combined vienna-ulm population of 51 karyotypically normal persons (see mikelsaar et al., 1977a). significant differences between the two populations in the frequency and patterns of a ... | 1979 | 92452 |
| idiotypic specificities and cross-reactivities of rabbit antibodies to human antidextran. | idiotypic antibodies were prepared by immunizing two groups of rabbits with dextran-antidextran specific precipitates and purified antidextran obtained subsequently from the same human donor. half of the animals were made tolerant to pooled human igg. tests showed that sera from tolerant rabbits reacted better with the antidextran preparation used to immunize the other group of animals than with the antidextran that formed part of their immunogen. non-tolerant animals did not recognize this sero ... | 1979 | 92456 |
| effect of leukocyte hydrolases on bacteria. xiv. bacteriolytic effects of human sera, synovial fluids, and purulent exudates on staphylococcus aureus and streptococcus faecalis: modulation by cohn's fraction ii and by polyelectrolytes. | normal sera and plasma, derived from humans, calves, rats, rabbits, horses, human synovial fluids, inflammatory exudates, and leukocyte extracts, when sufficiently diluted are highly bacteriolytic for staph, aureus, strep. faecalis, b. sutilis and to a variety of gram-negative rods. on the other hand, concentrated serum or the other body fluids are usually not bacteriolytic for these bacterial species. while the lysis of staph, aureus and b. subtilis by diluted serum is not lysozyme dependent, l ... | 1979 | 92458 |
| early detection of chlamydia trachomatis using fluorescent, dna binding dyes. | hela 229 cells were infected with genital tract strains of chlamydia trachomatis. after incubation for varying times the infected cells were fixed and stained with the fluorescent dna binding dyes hoechst 33258 or dapi for comparison with conventional giemsa stain. fluorochrome-treated preparations were examined by incident ultraviolet fluorescence microscopy and the giemsa-stained preparations by dark-ground light microscopy. chlamydial inclusion bodies could be identified unambiguously as earl ... | 1979 | 92480 |
| an investigation of the optimal conditions for the in vivo production of immunologically sensitised rat mast cells. | experiments were designed to develop an optimal method for inducing in vivo production of sensitised peritoneal mast cells. rats of different strains were sensitised with whole egg-white and killed at suitable intervals to harvest the peritoneal mast cells. release of histamine was induced in vitro by both whole egg-white and its major protein constituents, and assayed by a standard spectrofluorometric method. wistar rats showed higher levels of sensitisation than black-hooded lister rats; it wa ... | 1979 | 92507 |
| an electron microscopic india ink technique for demonstrating capsules on microorganisms: studies with streptococcus pneumoniae, staphylococcus aureus, and neisseria gonorrhoeae. | a technique using india ink in electron microscopic preparations was used to study bacterial capsules. capsules were demonstrated on streptococcus pneumoniae and staphylococcus aureus (strain m and the smith diffuse variant) from in vitro cultures. two types of false capsules were observed on neisseria gonorrhoeae grown in vitro or obtained from human urethral exudates. no true capsules were definitively identified on gonococci from these sources. the technique described does not require the use ... | 1979 | 92512 |
| unusual features in a case diagnosed as subacute sclerosing panencephalitis (sspe). | sspe is characterised by progressive mental deterioration, myoclonic and similar motor disorders and final severe comatose states, increase of immunoglobuline g in the csf, strongly elevated antibody titers to measles virus in serum and csf and typical periodic k-complexes in the eeg. the disease appears commonly in childhood and has a fatal course. cases with atypical signs have occasionally been reported. the case described in this paper shows a number of uncommon features: late onset, partial ... | 1979 | 92553 |
| characterization of intracellular precursor polyproteins of moloney murine leukemia virus. | intracellular moloney murine leukemia viral precursor polyproteins were compared with mature viral proteins by immunoprecipitation and tryptic peptide mapping experiments. the results were consistent with precursor roles for pr65gag, pr200gag-pol, pr135pol, and gpr83env. the glycosylated gag gene product gpr85gag, although containing sequences characteristic of all four core proteins plus additional sequences not found in pr65gag, lacked a major tyrosine-containing p30 tryptic peptide, suggestin ... | 1979 | 92574 |
| [recording cholinesterase and acetylcholinesterase activity on the "ferment-1" apparatus]. | | 1979 | 92607 |
| the accessibility of antigenic determinants of ribosomal protein s4 in situ. | antibodies to escherichia coli ribosomal protein s4 react with s4 in subribosomal particles, eg, the complex of 16s rna with s4, s7, s8, s15, s16, s17, and s19 and the ri reconstitution intermediate, but they do not react with intact 30s subunits. antibodies were isolated by three different methods from antisera obtained during the immunization of eight rabbits. some of these antibody preparations, which contained contaminant antibodies directed against other ribosomal proteins, reacted with sub ... | 1979 | 92615 |
| the carbohydrate content of apolipoprotein e from human very low density lipoproteins. | | 1979 | 92742 |
| an amino acid substitution in the gamma 1 chain of human immunoglobulin g associated with the gm (2) allotype. | | 1979 | 92755 |
| ionomycin stimulates mast cell histamine secretion by forming a lipid-soluble calcium complex. | the activation of cellular function following the direct introduction of ca2+ into the cytosol by the use of a ca2+-transporting ionophore has served to confirm the widely held idea that ca2+ has the status of a second messenger in many cell types. however, this evidence has been obtained largely from the use of a single ionophore, the antibiotic a23187; experiments with x537a, which is another ionophorous antibiotic capable of transporting ca2+ (ref. 3), have failed to show the expected charact ... | 1979 | 92764 |
| a specific projection of retinal displaced ganglion cells to the nucleus of the basal optic root in the chicken. | | 1979 | 92770 |
| the zinc iodide-osmium (zio) reaction in the central nervous system: localization and relations to functional activity. | the fine structural characteristics of zio reaction was studied in the cerebral and cerebellar cortex and olfactory bulb of the rat and in synaptosomes prepared from rat cerebellar cortex. it was concluded that: 1. organelles of different nerve cell types exhibit different zio reactions provided that the impregnation was carried out under standardized conditions. 2. 6...10 times more synaptic vesicles were stained by zio in the inhibitory terminals than in the excitatory ones. 3. zio positivity ... | 1979 | 92863 |
| metabolism of l-[u-14c; 2,3-3h] valine in perfused goat mammary glands [proceedings]. | | 1979 | 92967 |
| antigenic structure and biological characteristics of influenza strains isolated in the ussr during the 1974/75 epidemic. | antigenic analysis of strains isolated in the ussr during the epidemic in 1974/1975 showed differences in the hemagglutination inhibition test and in neuramidase activity with antisera against three reference strains. strains isolated in a later period of the epidemic were classified into subgroup a/port chalmers/1/73. nearly all strains were effective inducers of interferon and were susceptible to this inhibitor of virus replication. | 1979 | 92975 |
| isolation and cell culture propagation of rotaviruses from turkeys and chickens. | rotaviruses were detected by electron microscopy in the faeces of turkey poults and broiler chickens with diarrhoea. apparently symptomless infection was also observed in broilers. the avian rotaviruses could not be isolated in cell cultures by conventional techniques, but were adapted to serial growth in chick cell cultures following trypsin treatment of the virus and the cells. immunofluorescence studies showed that the avian and mammalian rotaviruses are antigenically related. antibodies to r ... | 1979 | 92979 |
| reaction of feblm with dna: fe(ii)blm-no. | | 1979 | 92990 |
| b-cell subsets responsive to fluorescein-conjugated antigens. iii. differential effect of e. coli lipopolysaccharide on t-dependent and t-independent responses in vivo. | the effect of e. coli lipopolysaccharide (lps) on the induction of both hapten-specific immunity and tolerance was studied in an in vivo system utilizing putative t-cell dependent (td) or t-cell independent (ti) challenge antigens. the administration of lps 1 day prior to challenge preempted the response of c3d2 mice to a td antigen (fl-klh) but had little effect on the response to fl-ficoll, a ti antigen. lps did not affect the responsiveness of c3h/hej mice, an lps-unresponsive strain, to eith ... | 1979 | 93080 |
| delayed hypersensitivity to staphylococcus aureus in mice: in vivo responses to isolated staphylococcal antigens. | the development of delayed hypersensitivity (dh) to staphylococcus aureus in swiss mice was evaluated by the footpad (fp) assay. in order to determine which component of the bacteria was responsible for the in vivo immune reactivity, purified staphylococcal cell wall, cell membrane, protein a, lipoteichoic acid, teichoic acid, as well as lipid-free membrane proteins were isolated. the immune responses of mice receiving one to eight s. aureus injections indicated that the first dh peak, following ... | 1979 | 93087 |
| biosynthesis of human blood group t-, n- and m-specific immunodeterminants on human erythrocyte antigens. | we synthesized on tn erythrocytes with human sera, udp-gal, and activators t-specific haptenic structures in satisfactory yield. the specificity of this biosynthesis was ascertained by agglutination with human and animal anti-t, by specific absorption of human anti-t as well as by agglutination inhibition assays. with isolated human erythrocyte t antigen as substrate we synthesized n- and m-specific structures with sera from individual human donors in presence of cmp-sialic acid by incubation fo ... | 1979 | 93134 |
| differences in the susceptibility of mhc and non-mhc mixed lymphocyte reactions to suppression by murine amnionic fluid and its components. | the ability of mouse amniotic fluid (maf), alpha-foetoprotein (afp) and maf depleted of afp (maf - afp) to suppress primary one-way mlr's was investigated. it was found that maf, afp and maf - afp were all suppressive of mlr's specific for mhc, k, d or i + s determinants. suppression was observed when either lymph node or spleen cells were used as the responder cells. nylon wool column passage of these cells did not significantly affect the immunosuppressive action of these substances. in contra ... | 1979 | 93135 |
| radioimmunological determination of serum 3 beta-hydroxy-5-cholenoic acid in normal subjects and patients with liver disease. | a radioimmunoassay for the determination of 3 beta-hydroxy-5-cholenoic acid in human serum has been developed, using 3 beta-hydroxy-5-cholenoyl-thyroglobulin as immunogen and 3 beta-hydroxy-5-cholenoylglycyl-125i histamine as radioactive ligand. the association constant was 6.3 x 10(8) l/mol. cross reactivity with other bile acids of human serum was not detectable, but was 5.6% with cholesterol. serum sample preparation included extraction of 3 beta-hydroxy-5-cholenoic acid from serum, solvolysi ... | 1979 | 93138 |
| immunological relationships among ovine glycoprotein hormones. | an antiserum to partially purified ovine fsh (but essentially free of lh) bound 125i-labelled ovine lh or bovine tsh. the antibody was directed exclusively against determinants in the alpha subunit. in a radioimmunoassay, only the intact ovine and bovine hormones and their alpha subunits were reactive; the hormone specific beta subunits exhibited no cross-reaction. the antibody directed against the alpha subunit was highly dependent on conformation. human lh, fsh, tsh or their alpha subunits did ... | 1979 | 93139 |
| influence of an oversulphated heparinoid upon hyaluronate metabolism of the human synovial cell in vivo. | glycosaminoglycans appear to affect proteoglycan metabolism of fibroblasts, human synovial cells, and chondrocytes under in vitro conditions. the influence of an oversulphated heparinoid (arteparon) on hyaluronate metabolism of the human synovial lining cell was studied by in vivo experiments on human volunteers. a higher production of better polymerized hyaluronic acid was observed after repeated intraarticular injections of this heparinoid into the knees of 2 osteoarthritic patients. after a s ... | 1979 | 93148 |
| glycosomes (protein-glycogen complex) in the canine heart. ultrastructure, histochemistry and changes induced by acidic treatment. | cardiac conducting fibers were selected from two dogs defined as a and b. the specimens differed in the reaction of their electron dense granules, commonly referred to as glycogen, to the treatment en bloc with uranyl acetate. material was fixed in glutaraldehyde and oso4. blocks were processed either conventionally or immersed in uranyl acetate before dehydration. sections were examined unsatined, stained with u and/or pb or with a histochemical technique (pa-tsc-sp) specific for glycogen. elec ... | 1979 | 93343 |
| quantitation of skin bacteria: lethality of the wash solution used to remove bacteria. | a widely used technique for the quantitative removal of bacteria from the skin uses a detergent, triton x-100 (p, t-octylphenoxynonaethoxyethanol), to remove and suspend the bacteria. we determined the half-life for the survival of five common skin bacteria suspended in the solution. the shortest-lived was streptococcus pyogenes with a half-life of 0.9 hours. all of the others (propionibacterium acnes, staphylococcus epidermidis, staphylococcus aureus, and klebsiella pneumoniae) had half-lives o ... | 1979 | 93373 |
| variations of cyclic amp and cyclic gmp contents of the liver and of brown and white adipose tissues of the rat during the first week of cold exposure. | the variation in the amounts of cyclic amp and cyclic gmp were studied in white and brown adipose tissues and in the liver of rats during the first week of cold exposure (5 degrees c). in white fat, only a small increase in camp was observed on the first day. in brown fat, parallel decreases in camp and cgmp contents were induced which might be related to a large mobilization of tissue fatty acids. in the liver, cold exposure barely affected the camp content but the level of cgmp was markedly in ... | 1979 | 93436 |
| study of the role of serotonin in lithium action using shock-elicited fighting. | | 1979 | 93531 |
| [covalently bound rna-dna molecules: the primary product of the reverse transcription of heterogeneous nuclear rna in normal cells]. | | 1979 | 93535 |
| recognition of protozoan parasite antigens by murine t lymphocytes. i. induction of specific t lymphocyte-dependent proliferative response to leishmania tropica. | | 1979 | 93546 |
| immunochemical studies on alcoholic hyalin. | | 1979 | 93559 |
| surface-associated host proteins on virulent treponema pallidum. | a surface coat of host serum proteins was detected on virulent treponema pallidum by sodium dodecyl sulfate-gel electrophoresis. the loosely associated serum proteins could be removed by repeated washings in a protein-free medium. washed t. pallidum retained the ability to readsorb numerous host proteins from rabbit serum as well as iodinated rabbit or human albumin. in addition, various avidly associated host serum proteins including albumin, alpha(2)-macroglobulin, transferrin, ceruloplasmin, ... | 1979 | 93574 |
| characteristics and genetic control of nk-cell-mediated cytotoxicity activated by naturally acquired infection in the mouse. | most and perhaps all natural-cell-mediated cytotoxicity (ncmc) may be activated by a response to exogenous infections. specific-pathogen-free (spf) mice have little or no natural killer (nk) cell activity, but when they are relocated in conventional conditions, they develop strong ncmc within 2 to 3 days. unlike other spf animals, hypothymic nude mice display good ncmc which is further augmented upon their entry into a pathogenic environment. the ontogeny, genetic control and other features of p ... | 1979 | 93585 |
| practical considerations in using counterimmunoelectrophoresis to identify the principal causative agents of bacterial meningitis. | many clinical laboratories are currently using counterimmunoelectrophoresis (cie) as an aid in the rapid diagnosis of bacterial meningitis. because cross-reactions among causative agents have been reported, the present study was undertaken to explore the problems that might occur when reference and commercial antisera are used in cie. broth cultures of 35 bacterial strains were tested with 76 reference and commercial antisera by cie. some of the antisera tested failed to react with their homolog ... | 1979 | 93606 |
| neutralizing antibody responses of humans and mice to vaccination with venezuelan encephalitis (tc-83) virus. | laboratory workers were vaccinated with venezuelan encephalitis virus, strain tc-83. after 2 to 3 years, they were bled, and their sera were tested for neutralizing antibody to all known venezuelan encephalitis subtypes and varieties. the results indicated higher titers to epizootic than to enzootic venezuelan encephalitis viruses and suggested that individuals vaccinated with tc-83 do not produce significant neutralizing antibody to heterologous subtypes. mice vaccinated, bled, and tested in a ... | 1979 | 93607 |
| comparison of auramine-rhodamine b and acridine orange for staining of acid-fast bacteria. | in cooperation of 6 laboratories in czechoslovakia and in the gdr, the efficiency of auramine-rhodamine b (ar) and acridine orange (ao) (short-time method) for staining of acid-fast bacilli was compared. whereas a former comparison of ar and ao (original method) pointed out the superiority of ar, the investigation of both methods used as short-time procedures showed significantly more acid-fast rods after using ao. the number of "false positive" results was somewhat higher on ar staining. howeve ... | 1979 | 93610 |
| mechanism of the inhibitory action of linoleic acid on the growth of staphylococcus aureus. | linoleic acid, but not stearic acid, inhibited the growth of staphylococcus aureus nctc 8325. growth inhibition was associated with an increase in the permeability of the bacterial membrane. the presence of a plasmid conferring resistance to penicillin (pc plasmid, e.g. pi258blai-) increased the growth inhibitory and membrane permeability effects of linoleic acid. under growth inhibitory conditions, linoleic acid was incorporated into the lipid of both pc plasmid-containing and pc plasmid-negati ... | 1979 | 93615 |
| the effect of an escherichia coli regulatory mutation on transfer rna structure. | | 1979 | 93644 |
| [partial purification of human pancreas specific antigen (author's transl)]. | | 1979 | 93659 |
| partial amino acid sequences of rabbit and rat beta 2-microglobulins. | | 1979 | 93696 |
| substance p as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. | | 1979 | 93706 |
| are histamine h2-receptor depressions of neuronal activity in tissue cultures from rat hypothalamus mediated through cyclic adenosine monophosphate? | iontophoretic application of histamine h2-agonists depressed spontaneous firing of tuberal hypothalamic neurons in tissue culture. perfusion with inhibitors of cyclic nucleotide phosphodiesterase increased the strength of these depressions, which persisted during perfusion with ca2+-free medium. these results support a role of cyclic amp as a mediator of histamine h2-elicited depressions of neuronal activity. | 1979 | 93718 |
| a unique population of uranaffin-positive intrinsic nerve endings in the small intestine. | a positive uranaffin reaction was observed in the small (40-60 nm) diameter vesicles of some intestinal axons. there was no change in the number of reactive axons or the intensity of reaction after reserpine (5 mg/kg) or after interruption of axons reaching the intestine through the mesentery. the axons were found in the myenteric, submucous and deep muscular plexuses and in the circular muscle. some uranaffin-positive axons formed synapses with neurons of the myenteric and submucous plexuses. i ... | 1979 | 93727 |
| double labelling of blanched neurons in the central nervous system of the rat by retrograde axonal transport of horseradish peroxidase and iron dextran complex. | the retrograde axonal transport of an iron-dextran complex leads to a labelling of neural cell bodies in the central nervous system (cns) of the rat. this tracer and horseradish peroxidase (hrp) can both be demonstrated histochemically in same cell bodies of intralaminar thalamic neurons in the central lateral nucleus, after injection of iron-dextran in the striatum and injection of hrp in the motor cortex. this is made possible by processing the sections first for hrp and then for ferric ions b ... | 1979 | 93728 |
| problems associated with prolonged wear soft contact lenses. | complications of prolonged wearing of soft contact lenses are corneal infection, peripheral vascularization, giant papillary conjunctivitis and allergy, sterile ulcers, corneal warping, lens infection, protein and calcium deposits, lens fragility, and problems with disinfecting solutions. further long-term studies are needed to compare the safety of prolonged wear soft contact lenses with the safety of intraocular lenses, on which long-term studies are currently being done. | 1979 | 93736 |
| rotational diffusion of band 3 in the red cell membrane: measurements using triplet probes. | rotational diffusion of band 3 in the human erythrocyte membrane has been investigated using the technique of flash photolysis. membranes are labeled with the triplet probe eosin isothiocyanate; most of the label is found to be associated with band 3. rotation is measured by observing the decay of dichroism of flash-induced absorbance changes arising from the eosin probe. the results indicate that band 3 rotates about an axis normal to the plane of the membrane with a diffusion coefficient in th ... | 1979 | 93746 |
| effects of intestinal amylase and trypsin on pancreatic secretion in the pig. | pigs were surgically prepared with external pancreatic fistulae and duodenal cannulae in order to elucidate whether the proposed intestinal feedback control of pancreatic secretion in the pig--as in rat and man--is exerted by trypsin. furthermore, the effect of intraluminal amylase on pancreatic secretion was studied. reintroduction of pancreatic juice into the duodenum or infusion of trypsin into the duodenum depressed the volume of the pancreatic flow and the protein output markedly. introduct ... | 1979 | 93774 |
| i-region-coded products expressed on both macrophages and thymus epithelium influence t-cell activities. | i-region-coded products expressed on the surface of both macrophages (mph) and thymus epithelium were shown to restrict t-cell activities at the level of antigen recognition and determination of the t-cell repertoire. thus keyhole limpet haemocyanin (klh) -pulsed mph could elicit a delayed-type hypersensitivity response in a klh-sensitized mouse only when the mph and the sensitized mouse were compatible at the i-a region of the major histocompatibility complex. also, experiments with thymus-graf ... | 1979 | 93776 |
| effect of chronic lead exposure on the direct and indirect components of the cardiac response to norepinephrine. | | 1979 | 93791 |
| in vitro primary immunization of human peripheral blood lymphocytes to klh: evidence for hla-d-region restriction. | | 1979 | 93813 |
| haematological changes in hyperimmunized rabbits treated with hydrazinophthalazines for long time periods. | haematological investigations of peripheral blood and bone marrow were performed in rabbits receiving hydrazinophthalazines (hydralazine or binazin) with or without simultaneous hyperimmunization by means of repeated injections of bovine albumin. it was found that long-term administration of hydralazine caused appearance of le factor in the serum and le cells in blood. less evident changes were caused by binazin administration. hyperimmunization caused weaknening of the effects of hydralazinopht ... | 1979 | 93848 |
| triggering of autolytic cell wall degradation in escherichia coli by beta-lactam antibiotics. | a biochemical method was developed to quantitatively compare the effectiveness of beta-lactams in triggering murein degradation (autolysin activity) in escherichia coli. bacteria prelabeled in their cell walls with radioactive diaminopimelic acid in growth medium were exposed for 10 min to the antibiotics at the appropriate minimal growth inhibitory concentrations and at multiples of these values, and the rate of cell wall degradation was followed during subsequent penicillin-binding protein (pb ... | 1979 | 93877 |