| genomic variations define divergence of water/wildlife-associated campylobacter jejuni niche specialists from common clonal complexes. | although the major food-borne pathogen campylobacter jejuni has been isolated from diverse animal, human and environmental sources, our knowledge of genomic diversity in c. jejuni is based exclusively on human or human food-chain-associated isolates. studies employing multilocus sequence typing have indicated that some clonal complexes are more commonly associated with particular sources. using comparative genomic hybridization on a collection of 80 isolates representing diverse sources and clon ... | 2011 | 21418497 |
| efficacy of uv light treatment for the microbiological decontamination of chicken, associated packaging, and contact surfaces. | uv light was investigated for the decontamination of raw chicken, associated packaging, and contact surfaces. the uv susceptibilities of a number of campylobacter isolates (seven campylobacter jejuni isolates and three campylobacter coli isolates), escherichia coli atcc 25922, and salmonella enterica serovar enteritidis atcc 10376 in liquid media were also investigated. from an initial level of 7 log cfu/ml, no viable campylobacter cells were detected following exposure to the most intense uv do ... | 2011 | 21477470 |
| antimicrobial resistance and molecular subtyping of campylobacter jejuni and campylobacter coli from retail meats. | campylobacter isolates (n = 297; 202 c. jejuni and 95 c. coli isolates) recovered from 2,513 retail meat samples (chicken breasts, ground turkey, ground beef, and pork chops) were examined for antimicrobial susceptibility. the isolates were further analyzed for genetic relatedness by pulsed-field gel electrophoresis (pfge) using smai and kpni restriction enzymes, and a subset of isolates (n = 174) were subtyped by multilocus sequence typing (mlst). the resistance most frequently observed was tha ... | 2011 | 21477476 |
| prevalence and risk factors for campylobacter spp. in chicken broiler flocks in reunion island (indian ocean). | our objectives were to determine campylobacter prevalence in broiler chicken flocks in reunion island and to define specific practices associated with the presence of campylobacter spp. infection in reunionese broiler flocks. fifty broiler flocks were studied in reunion island from may 2007 to february 2009. a questionnaire was submitted to the farmers and samples of fresh droppings were collected to assess the flock's campylobacter status. fifty four percent of the flocks were infected by campy ... | 2011 | 21511349 |
| antimicrobial susceptibility in thermophilic campylobacter species isolated from pigs and chickens in south africa. | campylobacter jejuni is one of the leading causes of sporadic food-borne bacterial disease in humans. in intensive poultry and pig rearing systems the use of oral antibiotics is essential to maintain health. consequently, there is a high risk for the thermophilic campylobacter jejuni and c. coli resident in the intestinal tract of food animals to develop resistance to commonly used antibiotics. contamination of meat or eggs with pathogenic strains of resistant campylobacter could, therefore, res ... | 2010 | 21526738 |
| prevalence and characterization of campylobacter jejuni isolated from pasture flock poultry. | the growing interest in organic and natural foods warrants a greater need for information on the food safety of these products. in this study, samples were taken from 2 pasture flock farms (n= 178; feed, water, drag swabs, and insect traps), pasture flock retail carcasses (n= 48) and 1 pasture flock processing facility (n= 16) over a period of 8 mo. a total of 105 campylobacter isolates were obtained from 53 (30%), 36 (75%), and 16 (100%) samples from the farms, retail carcasses, and processing ... | 2010 | 21535562 |
| effect of refrigerated and frozen storage on the survival of campylobacter jejuni in cooked chicken meat breast. | this experimental work aimed to examine the survivability of campylobacter jejuni in cooked chicken breast under several conditions: storage for 1, 3, and 7 d at refrigerated temperatures (4 °c) and for 20 d at frozen temperatures (-18 °c). in addition, storage at ambient temperature (26 to 28 °c) was involved. chicken samples were inoculated with a mixed culture of c. jejuni strains (atcc: 29428 and 33219) of known concentrations (50 and 500 cfu/g). bacterial cells were recovered and enumerated ... | 2011 | 21535688 |
| antimicrobial edible apple films inactivate antibiotic resistant and susceptible campylobacter jejuni strains on chicken breast. | campylobacter jejuni is the leading cause of bacterial diarrheal illness worldwide. many strains are now becoming multidrug resistant. apple-based edible films containing carvacrol and cinnamaldehyde were evaluated for bactericidal activity against antibiotic resistant and susceptible c. jejuni strains on chicken. retail chicken breast samples inoculated with d28a and h2a (resistant strains) and a24a (a sensitive strain) were wrapped in apple films containing cinnamaldehyde or carvacrol at 0.5%, ... | 2011 | 21535839 |
| campylobacter contamination of broiler caeca and carcasses at the slaughterhouse and correlation with salmonella contamination. | in order to estimate the prevalence of campylobacter spp. and salmonella spp. on broiler chicken carcasses and the prevalence of campylobacter spp. in caeca, 58 french slaughterhouses were investigated in 2008. enumeration of campylobacter spp. was also performed in order to study the relation between caeca and carcass contamination. a pool of 10 caeca and one carcass were collected from 425 different batches over a 12-month period in 2008. salmonella was isolated on 32 carcasses leading to a pr ... | 2011 | 21569927 |
| influence of cell surface hydrophobicity on attachment of campylobacter to abiotic surfaces. | this work aimed to investigate the influence of physicochemical properties and prior mode of growth (planktonic or sessile culture) on attachment of 13 campylobacter jejuni strains and 5 campylobacter coli strains isolated from chicken samples to three abiotic surfaces: stainless steel, glass and polyurethane. water contact angle and zeta potential measurements indicated that the strains varied with respect to surface hydrophobicity (17.6 ± 1.5 to 53.0 ± 2.3°) and surface charge (-3.3 ± 0.4 to - ... | 2011 | 21569937 |
| transmission and dose-response experiments for social animals: a reappraisal of the colonization biology of campylobacter jejuni in chickens. | dose-response experiments characterize the relationship between infectious agents and their hosts. these experiments are routinely used to estimate the minimum effective infectious dose for an infectious agent, which is most commonly characterized by the dose at which 50 per cent of challenged hosts become infected-the id(50). in turn, the id(50) is often used to compare between different agents and quantify the effect of treatment regimes. the statistical analysis of dose-response data typicall ... | 2011 | 21593028 |
| global gene expression analysis of chicken caecal response to campylobacter jejuni. | campylobacter jejuni colonises the caecum of more than 90% of commercial chickens. even though colonisation is asymptomatic, we hypothesised that it is mediated by activation of several biological pathways. we therefore used chicken-specific 20k oligonucleotide microarrays to examine global gene expression in c. jejuni-challenged birds. microarray results demonstrate small but significant fold-changes in expression of 270 genes 20h post-challenge, corresponding to a wide range of biological proc ... | 2011 | 21605915 |
| functional characterization of a lipoprotein-encoding operon in campylobacter jejuni. | bacterial lipoproteins have important functions in bacterial pathogenesis and physiology. in campylobacter jejuni, a major foodborne pathogen causing gastroenteritis in humans, the majority of lipoproteins have not been functionally characterized. previously, we showed by dna microarray that cmer, a transcriptional regulator repressing the expression of the multidrug efflux pump cmeabc, modulates the expression of a three-gene operon (cj0089, cj0090, and cj0091) encoding a cluster of lipoprotein ... | 2011 | 21625391 |
| exposure assessment and process sensitivity analysis of the contamination of campylobacter in poultry products. | studies were conducted in a thai poultry plant to identify the factors that affected numbers of campylobacter jejuni in chicken carcasses. the concentrations of campylobacter were determined using the simplate most probable number and modified charcoal cefoperazone deoxycholate plating methods. results indicated that the mean concentrations of c. jejuni in carcasses after scalding, plucking, and chilling were 2.93 ┬▒ 0.31, 2.98 ┬▒ 0.38, 2.88 ┬▒ 0.31, and 0.85 ┬▒ 0.95 log cfu, whereas the concent ... | 2011 | 21673173 |
| systemic response to campylobacter jejuni infection by profiling gene transcription in the spleens of two genetic lines of chickens. | campylobacter jejuni (c. jejuni) is a leading cause of human bacterial enteritis worldwide with poultry products being a major source of c. jejuni contamination. the chicken is the natural reservoir of c. jejuni where bacteria colonize the digestive tract of poultry, but rarely cause symptoms of disease. to understand the systemic molecular response mechanisms to c. jejuni infection in chickens, total splenic rna was isolated and applied to a whole genome chicken microarray for comparison betwee ... | 2011 | 21748442 |
| comparison of campylobacter jejuni isolates from human, food, veterinary and environmental sources in iceland using pfge, mlst and fla-svr sequencing. | campylobacter jejuni isolates from various sources in iceland were genotyped with the aim of assessing the genetic diversity, population structure, source distribution and campylobacter transmission routes to humans. | 2011 | 21740489 |
| transfer, viability and colonisation of campylobacter jejuni in the chicken vitellus and in embryos. | 1. the objective of this study was to evaluate the ability of campylobacter jejuni to penetrate and colonise eggs from specific-pathogen-free (spf) and heavy breeder hens, and to determine its effects on the viability of spf embryos. 2. we detected c. jejuni in 10% of breeder hens and 20% of spf eggs, which demonstrates the ability of the bacteria to go through the pores of eggs and contaminate the vitellus after 3ôçëh of contact. these results indicate that there is a risk of contamination unde ... | 2011 | 21732872 |
| prevalence of foodborne pathogens in grilled chicken from street vendors and retail outlets in reynosa, tamaulipas, mexico. | we analyzed a total of 70 grilled chicken samples bought randomly from street vendors and retail outlets in the city of reynosa, mexico, to determine the prevalence of escherichia coli (shiga toxin producing and enterotoxin producing), salmonella spp., staphylococcus aureus, listeria spp., and campylobacter spp. using microbiological methods and pcr detection of bacterial sequences. of the 70 samples, 27 (38.5%) were from retail outlets and 43 (61.4%) from street vendors. all specimens were nega ... | 2011 | 21819659 |
| irrelevance between the induction of anti-campylobacter humoral response by a bacterin and the lack of protection against homologous challenge in japanese jidori chickens. | on-farm vaccination of chickens against campylobacter jejuni is considered a potentially effective countermeasure to decrease campylobacteriosis via consumption of contaminated chicken meat, but is not yet available. in this study, two groups of jidori chicks were immunized subcutaneously with a formalin-killed c. jejuni with 2 different adjuvants. another chicks served as the unvaccinated control group. both vaccines induced high levels of anti-campylobacter igg, but provided no decrease of bac ... | 2011 | 21836379 |
| identification of persistent subtypes of campylobacter jejuni by pulsed-field gel electrophoresis in finland. | abstract the genetic diversity of campylobacter jejuni strains isolated from finnish patients was studied by typing 508 strains by pulsed-field gel electrophoresis: 311 were isolated from domestically acquired infections and 197 from travel-related infections. the strains were systematically selected from a larger collection of previously serotyped strains isolated during two 1-year sampling periods. the four most common smai profiles accounted for 45% of the domestic strains but only 3% of the ... | 2011 | 21675864 |
| colonization factors of campylobacter jejuni in the chicken gut. | abstract: campylobacter contaminated broiler chicken meat is an important source of foodborne gastroenteritis and poses a serious health burden in industrialized countries. broiler chickens are commonly regarded as a natural host for this zoonotic pathogen and infected birds carry a very high c. jejuni load in their gastrointestinal tract, especially the ceca. this eventually results in contaminated carcasses during processing. current intervention methods fail to reduce the colonization of broi ... | 2011 | 21714866 |
| the prevalence and genetic diversity of campylobacter spp. in domestic 'backyard' poultry in canterbury, new zealand. | campylobacteriosis is the most commonly notified illness in new zealand. whilst the importance of commercial poultry in campylobacteriosis is well established, little is known about the possible role of chickens kept at home as a direct animal/faecal contact or consumption exposure pathway. the aim of this study was to determine the prevalence and genetic diversity of campylobacter spp. in domestic backyard chicken flocks in the canterbury region of new zealand. poultry faecal samples were colle ... | 2011 | 21824372 |
| Prevalence of Campylobacter and Salmonella in raw chicken on retail sale in the republic of Ireland. | To assess the current risks to consumers from Campylobacter and Salmonella in raw chicken products sold in the Republic of Ireland, a retail survey was undertaken to define their prevalence. Samples (n = 510) were analyzed using protocols based on ISO 10272-1:2006 and ISO 6579:2002. Processor codes on pack labels showed that 67% of samples were produced in the Republic of Ireland and 25% in the United Kingdom. Salmonella was present in 5.1% of samples, but the eight serovars found caused less t ... | 2011 | 22054193 |
| pan-european monitoring of susceptibility to human-use antimicrobial agents in enteric bacteria isolated from healthy food-producing animals. | objectives: to determine the antimicrobial susceptibility of escherichia coli, salmonella, campylobacter and enterococcus from cattle, pigs and chickens across the european union (eu) using uniform methodology. methods: intestinal samples (1624) were taken at slaughter across five eu countries. bacteria were isolated in national laboratories, whilst mics were determined in a central laboratory for key antimicrobials used in human medicine. clinical resistance was based on clsi breakpoints and de ... | 2011 | 22210758 |
| a tolerogenic mucosal immune response leads to persistent campylobacter jejuni colonization in the chicken gut. | campylobacter enteritis is the most reported zoonotic disease in many developed countries where it imposes a serious health burden. campylobacter transmission to humans occurs primarily through the chicken vector. chicks are regarded as a natural host for campylobacter species and are colonized with c. jejuni in particular. but despite carrying a very high bacterial load in their gastrointestinal tract, these birds, in contrast to humans, do not develop pathological signs. it seems that in chick ... | 2011 | 21995731 |
| impaired fitness and transmission of macrolide-resistant campylobacter jejuni in its natural host. | campylobacter jejuni is a major zoonotic pathogen transmitted to humans via the food chain and is prevalent in chickens, a natural reservoir for this pathogenic organism. due to the importance of macrolide antibiotics in clinical therapy of human campylobacteriosis, development of macrolide resistance in campylobacter has become a concern for public health. to facilitate the control of macrolide-resistant campylobacter, it is necessary to understand if macrolide resistance affects the fitness an ... | 2011 | 22183170 |
| development and evaluation of cmec subunit vaccine against campylobacter jejuni. | campylobacter jejuni is the leading bacterial cause of human enteritis in many industrialized countries. there is no commercial vaccine against c. jejuni available to date. cmec is an essential outer membrane component of cmeabc multidrug efflux pump that plays a critical role in antibiotic resistance and in vivo colonization of c. jejuni. cmec is prevalent in c. jejuni strains and is dramatically induced and immunogenic in vivo. in this study, we analyzed cmec sequence homology, examined in vit ... | 2010 | 22140651 |
| prevalence and antimicrobial susceptibility of campylobacter spp. in live and dressed chicken in jordan. | abstract a total of 140 broiler flocks presented for slaughtering at amman slaughterhouse were tested for campylobacter spp. via collection of cloacal swabs from live birds, feathered skin samples at prescalding, and skin samples at postscalding (62°c or 57°c scalding temperature), postevisceration, and postchilling. the results indicated that 40% of the flocks tested by cloacal swabs, 34% at prescalding, 32% at post 57°c scalding, and 32% postevisceration were harboring campylobacter jejuni. ... | 2011 | 21988398 |
| occurrence of the invasion associated marker (iam) in campylobacter jejuni isolated from cattle. | abstract: background: the invasion associated marker (iam) has been detected in the majority of invasive campylobacter jejuni retrieved from humans. furthermore, the detection of iam in c. jejuni isolated from two important hosts, humans and chickens, suggested a role for this marker in c. jejuni's colonization of multiple hosts. however, no data exist regarding the occurrence of this marker in c. jejuni isolated from non-poultry food-animals such as cattle, an increasingly important source for ... | 2011 | 22208406 |
| effects of polyphosphate additives on the ph of processed chicken exudates and the survival of campylobacter. | campylobacter spp. are nutritionally fastidious organisms that are sensitive to normal atmospheric oxygen levels and lack homologues of common cold shock genes. at first glance, these bacteria seem ill equipped to persist within food products under processing and storage conditions; however, they survive in numbers sufficient to cause the largest number of foodborne bacterial disease annually. a mechanism proposed to play a role in campylobacter survival is the addition of polyphosphate-contain ... | 2011 | 22004823 |
| [quinolone resistant campylobacter jejuni strains isolated from humans and from poultry]. | eight quinolone resistant campylobacter jejuni strains isolated from humans with diarrheal disease were compared with 23 isolates from chicken and from laying hens. samples were cultured on selective agar in microaerophilia, identified by conventional tests, and conserved in 17% glycerol at -70 c. clones were determined by rapd-pcr employing the 1254 primer (stern nj). five patterns were obtained. patterns i, ii, and v were found in both poultry and human isolates. pattern i was obtained from po ... | 2011 | 21893445 |
| cmer-dependent gene cj0561c is induced more effectively by bile salts than the cmeabc efflux pump in both human and poultry campylobacter jejuni strains. | the multidrug efflux pump cmeabc described in the food-borne pathogen campylobacter jejuni was shown to be an important element of bile resistance and significant for successful colonization of chicken intestines. recently, another gene (cj0561c) strongly suppressed by the same repressor (cmer) that regulates expression of cmeabc was identified in c. jejuni nctc 11168. initial data suggested that, similarly to cmeabc, cj0561c could be induced by bile salts. in the present study, the occurrence o ... | 2011 | 21920435 |
| the critical role of luxs in the virulence of campylobacter jejuni in a guinea pig model of abortion. | previous studies on campylobacter jejuni have demonstrated the role of luxs in motility, cytolethal distending toxin production, agglutination and intestinal colonization, however its direct involvement in virulence has not been reported. in this study we demonstrate a direct role of luxs in the virulence of c. jejuni in two different animal hosts. the ia3902 strain, a highly virulent sheep abortion strain recently described by our laboratory, along with its isogenic luxs mutant and luxs complem ... | 2011 | 22144479 |
| closely related campylobacter jejuni strains from different sources reveal a generalist rather than a specialist lifestyle. | abstract: background: campylobacter jejuni and campylobacter coli are human intestinal pathogens of global importance. zoonotic transmission from livestock animals or animal-derived food is the likely cause for most of these infections. however, little is known about their general and host-specific mechanisms of colonization, or virulence and pathogenicity factors. in certain hosts, campylobacter species colonize persistently and do not cause disease, while they cause acute intestinal disease i ... | 2011 | 22122991 |
| immunopathology and th1/th2 immune response of campylobacter jejuni-induced paralysis resembling guillain-barré syndrome in chicken. | immunopathogenesis of campylobacter jejuni-associated guillain-barré syndrome (gbs) is not yet well established probably due to lack of experimental model. therefore, we studied the th1/th2 immune response and pathological changes in c. jejuni-induced chicken model for gbs. c. jejuni (5 × 10(9) cfu/ml) and placebo were fed to 30 chickens each. stools of all birds were negative for c. jejuni by culture and pcr before experiment. the birds were regularly assessed for disease symptoms up to 30 days ... | 2011 | 22102098 |
| phsysiological characterization of campylobacter jejuni under cold stresses conditions: its potential for public threat. | campylobacter jejuni is the major cause of human gastroenteritis worldwide. under stress conditions, c. jejuni can enter a viable but non-culturable (vbnc) state. we found that the c. jejuni was able to enter a vbnc state by prolonged incubation at 4℃. the standard isolation methods using pre-enrichment steps in bolton broth or preston broth could not detect the vbnc cells in spiked chicken meat. the transcription levels of virulence-associated genes (flaa, flab, cadf, ciab, cdta, cdtb and cdtc) ... | 2011 | 21891974 |
| contribution of tat system translocated phox to campylobacter jejuni phosphate metabolism and resilience to environmental stresses. | campylobacter jejuni is a common gastrointestinal pathogen that colonizes food animals; it is transmitted via fecal contamination of food, and infections in immune-compromised people are more likely to result in serious long-term illness. environmental phosphate is likely an important sensor of environmental fitness and the ability to obtain extracellular phosphate is central to the bacteria's core metabolic responses. phox is the sole alkaline phosphatase in c. jejuni, a substrate of the tat tr ... | 2011 | 22028859 |
| development and stability of bacteriocin resistance in campylobacter spp. | several bacteriocins (bcns) that were identified from chicken commensal bacteria dramatically reduced campylobacter colonization in poultry and are being directed toward on-farm control of this important foodborne human pathogen. a recent study has shown that bcn resistance in campylobacter jejuni is very difficult to develop in vitro. in this study, in vivo development and stability of bcn resistance in campylobacter was examined. | 2011 | 21973216 |
| identification of lactobacilli residing in chicken ceca with antagonism against campylobacter. | bacteriocins produced by lactobacillus salivarius have been recently recognized as a natural means to control campylobacter and salmonella in live poultry. this finding is of relevance since campylobacter jejuni and campylobacter coli are the predominant species isolated from poultry that are associated with human campylobacteriosis. in the present work, lactic acid bacteria (lab) isolated from the cecum of twenty tunisian chickens were identified and those isolates with antagonism against campy ... | 2011 | 22069154 |
| comparison of campylobacter contamination levels on chicken carcasses between modern and traditional types of slaughtering facilities in malaysia. | a total of 360 samples including fresh fecal droppings, neck skins, and swab samples was collected from 24 broiler flocks and processed by 12 modern processing plants in 6 states in malaysia. ninety samples from 10 traditional wet markets located in the same states as modern processing plants were also collected. microbiological isolation for campylobacter was performed following iso 10272-1:2006 (e). the overall rate of contamination for campylobacter in modern processing plants and in traditio ... | 2011 | 21891977 |
| genome sequences of two stress-tolerant campylobacter jejuni poultry strains, 305 and dfvf1099. | campylobacter jejuni is a food-borne pathogen with a high prevalence in poultry meat, which in fresh unfrozen condition is the major source of campylobacteriosis. c. jejuni strains dfvf1099 and 305 are considered tolerant to several environmental stresses (t. birk et al., j. food prot. 73:258-265, 2010; s. l. on et al., int. j. med. microbiol. 296:353-363, 2006). here, we report the genome sequences of c. jejuni 305 and dfvf1099, a turkey and a chicken isolate, respectively. | 2011 | 21914871 |
| poultry as a host for the zoonotic pathogen campylobacter jejuni. | abstract campylobacteriosis is the most reported foodborne gastroenteritic disease and poses a serious health burden in industrialized countries. disease in humans is mainly caused by the zoonotic pathogen campylobacter jejuni. due to its wide-spread occurrence in the environment, the epidemiology of campylobacter remains poorly understood. it is generally accepted, however, that chickens are a natural host for campylobacter jejuni, and for campylobacter spp. in general, and that colonized bro ... | 2011 | 22133236 |
| Reverse transcriptase real-time PCR for detection and quantification of viable Campylobacter jejuni directly from poultry faecal samples. | Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of viable Campylobacter jejuni directly from chicken faecal samples. The results of this method and a DNA-based quantitative real-time PCR (qPCR) method were compared with those of a ... | 2011 | 22064380 |
| from farm to fork follow-up of thermotolerant campylobacters throughout the broiler production chain and in human cases in a hungarian county during a ten-months period. | a study tracking thermotolerant campylobacters from the setting of the broilers throughout the whole rearing period, slaughter and sale of chicken products in five consecutive broiler rotations of the same henhouse as well as in two different other farms was conducted in a well-defined geographic area (hajdú-bihar county, hungary) between march 2006 and feb 2007. all notified cases of human campylobacteriosis in this area during the study period were also included. one hundred and one, 44, 23 an ... | 2011 | 21864930 |
| Improvement of Modified Charcoal-Cefoperazone-Deoxycholate Agar by Supplementation with a High Concentration of Polymyxin B for the Detection of Campylobacter jejuni and C. coli in Chicken Carcass Rinse. | Modified charcoal-cefoperazone-deoxycholate agar (mCCDA) was improved by supplementation with a high concentration of polymyxin B. The ability of the supplemented medium to isolate Campylobacter jejuni and C. coli from chicken carcass rinse was compared to that of Campy-Cefex agar and mCCDA. Modification of mCCDA with increased polymyxin B yielded significantly (p < 0.05) better isolation rate and selectivity than those achieved on using Campy-Cefex agar and mCCDA. | 2011 | 22210208 |
| The cinnamon-oil ingredient trans-cinnamaldehyde fails to target Campylobacter jejuni strain KC 40 in the broiler chicken cecum despite marked in vitro activity. | Campylobacter jejuni is the most common bacterial cause of diarrheal disease in humans worldwide, with poultry products being a major source. Therefore, strategies to decrease Campylobacter colonization during primary production might aid in reducing the number of human campylobacteriosis cases. Several plant-derived compounds have been reported to possess anti-Campylobacter properties in vitro, so they could be promising candidates to reduce Campylobacter colonization in broiler chickens. To t ... | 2011 | 22004822 |
| caecal transcriptome analysis of colonized and non-colonized chickens within two genetic lines that differ in caecal colonization by campylobacter jejuni. | campylobacter jejuni is one of the most common causes of human bacterial enteritis worldwide. the molecular mechanisms of the host responses of chickens to c. jejuni colonization are not well understood. we have previously found differences in c. jejuni colonization at 7-days post-inoculation (pi) between two genetic broiler lines. however, within each line, not all birds were colonized by c. jejuni (27.5% colonized in line a, and 70% in line b). therefore, the objective of the present experimen ... | 2011 | 21906100 |
| treatment of raw poultry with nonthermal dielectric barrier discharge plasma to reduce campylobacter jejuni and salmonella enterica. | nonthermal plasma has been shown to be effective in reducing pathogens on the surface of a range of fresh produce products. the research presented here investigated the effectiveness of nonthermal dielectric barrier discharge plasma on salmonella enterica and campylobacter jejuni inoculated onto the surface of boneless skinless chicken breast and chicken thigh with skin. chicken samples were inoculated with antibiotic-resistant strains of s. enterica and c. jejuni at levels of 10(1) to 10(4) cf ... | 2012 | 22221351 |
| detection and quantification of campylobacter jejuni and campylobacter coli using real-time multiplex pcr. | aims: we describe a real-time multiplex pcr (qmpcr) assay to identify and discriminate between isolates of campylobacter jejuni and campylobacter coli. methods and results: two novel sets of primers and hydrolysis probes were designed to amplify the unique dna sequences within the hipo, ccon and cadf genes that are specific to c. jejuni and c. coli. by using the designed optimized qmpcr assay conditions, the amplification efficiency is in range from 108 to 116%. these qmpcr assays are highly s ... | 2012 | 22256961 |
| presence and characterization of campylobacter jejuni in organically raised chickens in quebec. | the objective of this study was to estimate the presence of the important foodborne pathogen campylobacter jejuni in organically raised chickens in the province of quebec. the recovered isolates were further characterized for their antimicrobial resistance profile, autoagglutination property and chemotaxis. antimicrobial resistance was evaluated using agar dilution for: tetracycline, erythromycin, chloramphenicol, ciprofloxacin, gentamicin, nalidixic acid, clindamycin, ampicillin, azithromycin, ... | 2011 | 22468028 |
| toward integrative genomics study of genetic resistance to salmonella and campylobacter intestinal colonization in fowl. | salmonella enterica serotypes enteritidis and typhimurium and campylobacter jejuni are responsible for most cases of food poisoning in europe. these bacteria do not cause severe disease symptoms in chicken, but they are easily propagated by symptomless chicken carriers which cannot be easily isolated. this animal tolerance is detrimental to food safety. in this particular case, increasing animal's resistance is not sufficient, since some animals considered as resistant are able to carry bacteria ... | 2012 | 23412643 |
| [campylobacter spp.: prevalence and pheno-genotypic characterization of isolates recovered from patients suffering from diarrhea and their pets in la pampa province, argentina]. | the prevalence of campylobacter spp. was investigated in 327 patients suffering from diarrhea and in 36 animals (dogs, cats and chickens) owned by the patients that presented infection by campylobacter in santa rosa, la pampa, argentina. campylobacter spp. was isolated in 50/327 patients and in 12/36 animals, being campylobacter jejuni the most common species. resistance to ciprofloxacin (65 %) and tetracycline (32 %) was found among 35 isolates of human origin studied. seven genetic subtypes we ... | 2012 | 23267623 |
| evaluation of the immunogenicity of campylobacter jejuni cjaa protein delivered by salmonella enterica sv. typhimurium strain with regulated delayed attenuation in chickens. | campylobacter spp. are regarded as the most common bacterial cause of gastroenteritis worldwide, and consumption of chicken meat contaminated by campylobacter is considered to be one of the most frequent sources of human infection in developed countries. here we evaluated the immunogenicity and protective efficacy of salmonella typhimurium χ9718 producing the campylobacter jejuni cjaa protein as a chicken anti-campylobacter vaccine. in this study chickens were orally immunized with a new generat ... | 2013 | 23913025 |
| evaluation of a protective effect of in ovo delivered campylobacter jejuni omvs. | campylobacter jejuni is the most prevalent cause of a food-borne gastroenteritis in the developed world, with poultry being the main source of infection. campylobacter jejuni, like other gram-negative bacteria, constitutively releases outer membrane vesicles (omvs). omvs are highly immunogenic, can be taken up by mammalian cells, and are easily modifiable by recombinant engineering. we have tested their usefulness for an oral (in ovo) vaccination of chickens. four groups of 18-day-old chicken em ... | 2016 | 27383607 |
| prevalence and antimicrobial resistance of thermophilic campylobacter isolated from chicken in côte d'ivoire. | thermophilic campylobacters are major causes of gastroenteritis in human. the main risk factor of infection is consumption of contaminated or by cross-contaminated poultry meat. in côte d'ivoire, gastroenteritis is usually observed but no case of human campylobacteriosis has been formally reported to date. the aims of this study were to determine prevalence and antimicrobial resistance of campylobacter jejuni and campylobacter coli isolated from chickens ceaca in commercial slaughter in abidjan. ... | 2012 | 23133454 |
| identification of a novel genomic island conferring resistance to multiple aminoglycoside antibiotics in campylobacter coli. | historically, the incidence of gentamicin resistance in campylobacter has been very low, but recent studies reported a high prevalence of gentamicin-resistant campylobacter isolated from food-producing animals in china. the reason for the high prevalence was unknown and was addressed in this study. pcr screening identified aminoglycoside resistance genes apha-3 and apha-7 and the aade-sat4-apha-3 cluster among 41 campylobacter isolates from broiler chickens. importantly, a novel genomic island c ... | 2012 | 22869568 |
| antimicrobial susceptibility profiles and molecular typing of campylobacter jejuni and campylobacter coli isolates from ducks in south korea. | campylobacter is a food-borne zoonotic pathogen that causes human gastroenteritis worldwide. campylobacter bacteria are commensal in the intestines of many food production animals, including ducks and chickens. the objective of the study was to determine the prevalence of campylobacter species in domestic ducks, and the agar dilution method was used to determine resistance of the isolates to eight antibiotics. in addition, multilocus sequence typing (mlst) was performed to determine the sequence ... | 2014 | 25261524 |
| evolution and comparative genomics of campylobacter jejuni st-677 clonal complex. | campylobacter is the most common bacterial cause of gastroenteritis in the european union with over 200,000 laboratory-confirmed cases reported annually. this is the first study to describe findings related to comparative genomics analyses of the sequence type (st)-677 clonal complex (cc), a campylobacter jejuni lineage associated with bacteremia cases in humans. we performed whole-genome sequencing, using illumina hiseq sequencing technology, on five related st-677 cc isolates from two chicken ... | 2014 | 25193305 |
| high-throughput sequencing of campylobacter jejuni insertion mutant libraries reveals mapa as a fitness factor for chicken colonization. | campylobacter jejuni is a leading cause of gastrointestinal infections worldwide, due primarily to its ability to asymptomatically colonize the gastrointestinal tracts of agriculturally relevant animals, including chickens. infection often occurs following consumption of meat that was contaminated by c. jejuni during harvest. because of this, much interest lies in understanding the mechanisms that allow c. jejuni to colonize the chicken gastrointestinal tract. to address this, we generated a c. ... | 2014 | 24633877 |
| detection, identification and quantification of campylobacter jejuni, coli and lari in food matrices all at once using multiplex qpcr. | thermotolerant campylobacter jejuni, coli and lari are recognized as leading food-borne pathogens causing an acute bacterial enteritis worldwide. due to narrow spectrum of their biochemical activity, it is very complicated to distinguish between individual species. for reliable risk assessment, proper incidence evaluation or swift sample analysis regarding individual species, a demand for simple and rapid method for their distinguishing is reasonable. in this study, we evaluated a reliable and s ... | 2014 | 25057300 |
| comparative analysis and limitations of ethidium monoazide and propidium monoazide treatments for the differentiation of viable and nonviable campylobacter cells. | the lack of differentiation between viable and nonviable bacterial cells limits the implementation of pcr-based methods for routine diagnostic approaches. recently, the combination of a quantitative real-time pcr (qpcr) and ethidium monoazide (ema) or propidium monoazide (pma) pretreatment has been described to circumvent this disadvantage. in regard to the suitability of this approach for campylobacter spp., conflicting results have been reported. thus, we compared the suitabilities of ema and ... | 2014 | 24487529 |
| alternative bacteriophage life cycles: the carrier state of campylobacter jejuni. | members of the genus campylobacter are frequently responsible for human enteric disease, often through consumption of contaminated poultry products. bacteriophages are viruses that have the potential to control pathogenic bacteria, but understanding their complex life cycles is key to their successful exploitation. treatment of campylobacter jejuni biofilms with bacteriophages led to the discovery that phages had established a relationship with their hosts typical of the carrier state life cycle ... | 2014 | 24671947 |
| prevalence of virulence genes in strains of campylobacter jejuni isolated from human, bovine and broiler. | campylobacter jejuni isolates of different origins (bovine, broiler meat, human) were screened by polymerase chain reaction for the presence of 4 genes cdtb, cst-ii, ggt, and virb11, previously linked to virulence such as adherence, invasion, colonization, molecular mimicry, and cytotoxin production. in addition, the isolates were screened for the presence of the global gene regulator csra linked to oxidative stress responses, biofilms formation, and cell adhesion. all the c. jejuni isolates wer ... | 2014 | 24688515 |
| biological roles of the o-methyl phosphoramidate capsule modification in campylobacter jejuni. | campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (cps) of this organism is required for persistence and disease. c. jejuni produces over 47 different capsular structures, including a unique o-methyl phosphoramidate (meopn) modification present on most c. jejuni isolates. although the meopn structure is rare in nature it has structural similarity to some synthetic pesticides. in this study, we have demonstrated, by whole genome comparis ... | 2014 | 24498018 |
| survival in water of campylobacter jejuni strains isolated from the slaughterhouse. | campylobacter jejuni cause gastroenteritis in humans. the main transmission vector is the consumption or handling of contaminated chicken meat, since chicken can be colonized asymptomatically by c. jejuni. however, water has been implicated as the transmission vector in a few outbreaks. one possibility is the contamination of water effluent by c. jejuni originating from chicken farm. the ability of c. jejuni to be transmitted by water would be closely associated to its ability to survive in wate ... | 2015 | 26702388 |
| molecular identification of campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being. | campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species. the aim of this study was to identify campylobacter jejuni and campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (pcr). | 2015 | 27047055 |
| novel immunomodulatory flagellin-like protein flac in campylobacter jejuni and other campylobacterales. | the human diarrheal pathogens campylobacter jejuni and campylobacter coli interfere with host innate immune signaling by different means, and their flagellins, flaa and flab, have a low intrinsic property to activate the innate immune receptor toll-like receptor 5 (tlr5). we have investigated here the hypothesis that the unusual secreted, flagellin-like molecule flac present in c. jejuni, c. coli, and other campylobacterales might activate cells via tlr5 and interact with tlr5. flac shows striki ... | 2015 | 27303676 |
| pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in campylobacter jejuni. | campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by c. jejuni and that a secreted protease, cj0511, is required. exposure of c. jejuni to pancreatic amylase promotes biofilm formation in vitro, increases interaction with human epithelial cell lines, increases virulence in the galleria mellonella infection mode ... | 2015 | 26438798 |
| differentiation of campylobacter jejuni and campylobacter coli using multiplex-pcr and high resolution melt curve analysis. | campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. among campylobacter spp. campylobacter jejuni (c. jejuni) and c. coli are the most common causes of human infection. in this study, a multiplex pcr (mpcr) and high resolution melt (hrm) curve analysis were optimized for simultaneous detection and differentiation of c. jejuni and c. coli isolates. a segment of the hippuricase gene (hipo) of c. jejuni and putative aspartokinase (asp) gene of c. c ... | 2015 | 26394042 |
| rapid host switching in generalist campylobacter strains erodes the signal for tracing human infections. | campylobacter jejuni and campylobacter coli are the biggest causes of bacterial gastroenteritis in the developed world, with human infections typically arising from zoonotic transmission associated with infected meat. because campylobacter is not thought to survive well outside the gut, host-associated populations are genetically isolated to varying degrees. therefore, the likely origin of most strains can be determined by host-associated variation in the genome. this is instructive for characte ... | 2015 | 26305157 |
| virulence characteristics of hcp (+) campylobacter jejuni and campylobacter coli isolates from retail chicken. | recently the type vi secretion system (t6ss), which can play a significant role in bacterial survival and pathogenesis, was reported in campylobacter spp., having the hcp gene as a key component. | 2015 | 26207145 |
| narrow-spectrum inhibitors of campylobacter jejuni flagellar expression and growth. | campylobacter jejuni is a major cause of food-borne illness due to its ability to reside within the gastrointestinal tracts of chickens. multiple studies have identified the flagella of c. jejuni as a major determinant of chicken colonization. an inhibitor screen of approximately 147,000 small molecules was performed to identify compounds that are able to inhibit flagellar expression in a reporter strain of c. jejuni. several compounds that modestly inhibited motility of wild-type c. jejuni in s ... | 2015 | 25870073 |
| genetic characterization of campylobacter jejuni and c. coli isolated from broilers using flaa pcr-restriction fragment length polymorphism method in shiraz, southern iran. | thermophilic campylobacters, particularly campylobacter jejuni and c. coli are the main agents of human campylobacteriosis. campylobacter contaminated chicken products is the most important source of foodborne gastroenteritis. evaluation of genetic diversity among campylobacter population is critical for understanding the epidemiology of this bacterium and developing effective control strategies against campylobacter infections and other related disorders. | 2015 | 26060566 |
| epidemiology and rapd-pcr typing of thermophilic campylobacters from children under five years and chickens in morogoro municipality, tanzania. | campylobacter species are gram negative and flagellated bacteria under the genus campylobacter, family campylobacteriaceae. these pathogens cause zoonotic infections among human and animal populations. this study was undertaken between december 2006 and may 2007 to determine prevalence, risk factors and genetic diversity of thermophilic campylobacter isolates from children less than 5 years and chickens in morogoro municipality, tanzania. | 2016 | 27871251 |
| molecular epidemiology, virulence determinants and antimicrobial resistance of campylobacter spreading in retail chicken meat in central china. | campylobacter species are the major food-borne pathogens which could cause bacterial gastroenteritis in humans. contaminated chicken products have been recognized as the primary vehicles of campylobacter transmission to human beings. in this study, the prevalence of campylobacter in retail chicken meat in central china was investigated, and the isolates were further characterized using molecular approaches and tested for antibiotic resistance. | 2016 | 27800028 |
| a direct-sensing galactose chemoreceptor recently evolved in invasive strains of campylobacter jejuni. | a rare chemotaxis receptor, tlp11, has been previously identified in invasive strains of campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that tlp11 specifically interacts with galactose. tlp11 is required for the chemotactic response of c. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. the inactivated mutant displays reduced ... | 2016 | 27762269 |
| use of direct lamp screening of broiler fecal samples for campylobacter jejuni and campylobacter coli in the positive flock identification strategy. | rapid identification of campylobacter-positive flocks before slaughter, following freezing and heat treatment for the campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. we evaluated a loop-mediated isothermal amplification (lamp) assay for the direct screening of naturally contaminated chicken cloacal swabs for c. jejuni/c. coli to compare this assay with conventional quantitative culture methods. in a comparison study o ... | 2016 | 27746777 |
| quantification of growth of campylobacter and extended spectrum β-lactamase producing bacteria sheds light on black box of enrichment procedures. | campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide, and is routinely found in meat originating from poultry, sheep, pigs, and cattle. effective monitoring of campylobacter contamination is dependent on the availability of reliable detection methods. the method of the international organization for standardization for the detection of campylobacter spp. in food (iso 10272-1:2006) recommends the use of bolton broth (bb) as selective enrichment ... | 2016 | 27672384 |
| identification of novel vaccine candidates against campylobacter through reverse vaccinology. | campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the european union. human cases are mainly due to campylobacter jejuni or campylobacter coli, and contamination is associated with the handling and/or consumption of poultry meat. in fact, poultry constitutes the bacteria's main reservoir. a promising way of decreasing the incidence of campylobacteriosis in humans would be to decrease avian colonization. poultry vaccination is of potential for this p ... | 2016 | 27413761 |
| whole-genome sequencing of a campylobacter jejuni strain isolated from retail chicken meat reveals the presence of a megaplasmid with mu-like prophage and multidrug resistance genes. | genome sequencing of campylobacter jejuni strain t1-21 isolated from retail chicken meat revealed the presence of a chromosome of 1,565,978 bp and a megaplasmid of 82,732 bp that contains mu-like prophage and multidrug resistance genes. this is the first reported sequence of a campylobacter megaplasmid >55 kb. | 2016 | 27231378 |
| campylobacter in broiler slaughter samples assessed by direct count on mccda and campy-cefex agar. | campylobacter spp. cause foodborne illnesses in humans primarily through the consumption of contaminated chicken. the aim of this study was to evaluate the united states department of agriculture's (usda) recommended methodology, protocol mlg 41.02, for the isolation, identification and direct plate counting of campylobacter jejuni and c. coli samples from the broiler slaughtering process. a plating method using both mccda and campy-cefex agars is recommended to recover campylobacter cells. it i ... | 2016 | 27237112 |
| effect of refrigeration and frozen storage on the campylobacter jejuni recovery from naturally contaminated broiler carcasses. | campylobacter jejuni is the most common thermophilic campylobacter associated with human enteritis in many countries. broilers and their by-products are the main sources for human enteritis. refrigeration and freezing are used to control bacterial growth in foods. the effect of these interventions on survival of campylobacter jejuni is yet not quite understood. this study evaluated the effect of storage temperature on the survival of c. jejuni in chicken meat stored for seven days at 4°c and for ... | 2010 | 24031523 |
| molecular-based detection of the gastrointestinal pathogen campylobacter ureolyticus in unpasteurized milk samples from two cattle farms in ireland. | campylobacter jejuni and coli are collectively regarded as the most prevalent cause of bacterial foodborne illness worldwide. an emerging species, campylobacter ureolyticus has recently been detected in patients with gastroenteritis, however, the source of this organism has, until now, remained unclear. herein, we describe the molecular-based detection of this pathogen in bovine faeces (1/20) and unpasteurized milk (6/47) but not in poultry (chicken wings and caeca). this is, to the best of our ... | 2012 | 23151337 |
| avian resistance to campylobacter jejuni colonization is associated with an intestinal immunogene expression signature identified by mrna sequencing. | campylobacter jejuni is the most common cause of human bacterial gastroenteritis and is associated with several post-infectious manifestations, including onset of the autoimmune neuropathy guillain-barré syndrome, causing significant morbidity and mortality. poorly-cooked chicken meat is the most frequent source of infection as c. jejuni colonizes the avian intestine in a commensal relationship. however, not all chickens are equally colonized and resistance seems to be genetically determined. we ... | 2012 | 22870198 |
| association of campylobacter jejuni metabolic traits with multilocus sequence types. | in this study, we describe the association of three campylobacter jejuni metabolism-related traits, γ-glutamyl-transpeptidase (ggt), fucose permease (fucp), and secreted l-asparaginase [ansb(s)], with multilocus sequence types (sts). a total of 710 c. jejuni isolates with known sts were selected and originated from humans, poultry, bovines, and the environment. among these isolates, we found 31.1% to produce ggt and 49.3% and 30.3% to be positive for ansb(s) and fucp, respectively. the combinati ... | 2012 | 22660710 |
| zinc competition among the intestinal microbiota. | bioavailable levels of trace metals, such as iron and zinc, for bacterial growth in nature are sufficiently low that most microbes have evolved high-affinity binding and transport systems. the microbe campylobacter jejuni lives in the gastrointestinal tract of chickens, the principal source of human infection. a high-affinity abc transporter for zinc uptake is required for campylobacter survival in chicken intestines in the presence of a normal microbiota but not when chickens are raised with a ... | 2012 | 22851657 |
| passage of campylobacter jejuni through the chicken reservoir or mice promotes phase variation in contingency genes cj0045 and cj0170 that strongly associates with colonization and disease in a mouse model. | human illness due to camplyobacter jejuni infection is closely associated with consumption of poultry products. we previously demonstrated a 50 % shift in allele frequency (phase variation) in contingency gene cj1139 (wlan) during passage of c. jejuni nctc11168 populations through ross 308 broiler chickens. we hypothesized that phase variation in contingency genes during chicken passage could promote subsequent colonization and disease in humans. to test this hypothesis, we passaged c. jejuni st ... | 2012 | 22343355 |
| antimicrobial activities of isothiocyanates against campylobacter jejuni isolates. | food-borne human infection with campylobacter jejuni is a medical concern in both industrialized and developing countries. efficient eradication of c. jejuni reservoirs within live animals and processed foods is limited by the development of antimicrobial resistances and by practical problems related to the use of conventional antibiotics in food processes. we have investigated the bacteriostatic and bactericidal activities of two phytochemicals, allyl-isothiocyanate (aitc), and benzyl isothiocy ... | 2012 | 22919644 |
| phase variable genes of campylobacter jejuni exhibit high mutation rates and specific mutational patterns but mutability is not the major determinant of population structure during host colonization. | phase variation of surface structures occurs in diverse bacterial species due to stochastic, high frequency, reversible mutations. multiple genes of campylobacter jejuni are subject to phase variable gene expression due to mutations in polyc/g tracts. a modal length of nine repeats was detected for polyc/g tracts within c. jejuni genomes. switching rates for these tracts were measured using chromosomally-located reporter constructs and high rates were observed for cj1139 (g8) and cj0031 (g9). al ... | 2012 | 22434884 |
| colonization properties of campylobacter jejuni in chickens. | campylobacter is the most common bacterial food-borne pathogen worldwide. poultry and specifically chicken and raw chicken meat is the main source for human campylobacter infection. whilst being colonized by campylobacter spp. chicken in contrast to human, do scarcely develop pathological lesions. the immune mechanisms controlling campylobacter colonization and infection in chickens are still not clear. previous studies and our investigations indicate that the ability to colonize the chicken var ... | 2012 | 24611122 |
| identification and antimicrobial resistance of campylobacter species isolated from animal sources. | campylobacter spp. are together with salmonella spp. the leading causes of human bacterial gastroenteritis worldwide. the most commonly isolated species in humans are campylobacter jejuni and c. coli. the isolation, identification, and antimicrobial resistance of campylobacter spp. from poultry and raw meat from slaughterhouses, has been investigated for the first time in greece. during the period from august 2005 to november 2008 a total of 1080 samples were collected: (a) 830 fecal samples fro ... | 2012 | 22375138 |
| phase variable expression of capsular polysaccharide modifications allows campylobacter jejuni to avoid bacteriophage infection in chickens. | bacteriophages are estimated to be the most abundant entities on earth and can be found in every niche where their bacterial hosts reside. the initial interaction between phages and campylobacter jejuni, a common colonizer of poultry intestines and a major source of foodborne bacterial gastroenteritis in humans, is not well understood. recently, we isolated and characterized a phage f336 resistant variant of c. jejuni nctc11168 called 11168r. comparisons of 11168r with the wildtype lead to the i ... | 2012 | 22919603 |
| defense and adaptation: the complex inter-relationship between campylobacter jejuni and mucus. | mucus colonization is an essential early step toward establishing successful infection and disease by mucosal pathogens. there is an emerging literature implicating specific mucin sub-types and mucin modifications in protecting the host from campylobacter jejuni infection. however, mucosal pathogens have evolved sophisticated mechanisms to breach the mucus layer and c. jejuni in particular appears to harbor specific adaptations to better colonize intestinal mucus. for example, components of mucu ... | 2012 | 22919607 |
| extreme heat resistance of food borne pathogens campylobacter jejuni, escherichia coli, and salmonella typhimurium on chicken breast fillet during cooking. | the aim of this research was to determine the decimal reduction times of bacteria present on chicken fillet in boiling water. the experiments were conducted with campylobacter jejuni, salmonella, and escherichia coli. whole chicken breast fillets were inoculated with the pathogens, stored overnight (4°c), and subsequently cooked. the surface temperature reached 70°c within 30 sec and 85°c within one minute. extremely high decimal reduction times of 1.90, 1.97, and 2.20 min were obtained for c. j ... | 2012 | 22389647 |
| detection and species identification of campylobacter in stool samples of children and animals from vellore, south india. | campylobacter spp. are an important cause of bacterial gastroenteritis frequently isolated from animal, poultry and environmental samples. in this study, we investigated the zoonotic potential of campylobacter spp. by comparing prevalence rates and species in 394 children with diarrhoea and 652 animals in vellore using pcr-based tools. eighteen children (4.5%) had campylobacteriosis, a majority of whom had co-pathogens (15/18) and most were infected with campylobacter jejuni (16/18). a few c. co ... | 2012 | 22361767 |
| pentavalent single-domain antibodies reduce campylobacter jejuni motility and colonization in chickens. | campylobacter jejuni is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. contaminated poultry meat is a major source of c. jejuni infection, and therefore, strategies to reduce this organism in poultry, are expected to reduce the incidence of campylobacter-associated diseases. we have investigated whether oral administration of c. jejuni-specific single-domain antibodies would reduce bacterial colonization ... | 2013 | 24391847 |
| virulence characteristics of five new campylobacter jejuni chicken isolates. | campylobacter enteritis has emerged as one of the most common forms of human diarrheal illness. in this study we have investigated the virulence potential of five new c. jejuni chicken isolates (ro14, ro19, ro24, ro29 and ro37) originated from private households in the rural regions of banat and transylvania in romania. following isolation and in vitro virulence assay, on hct-8 cells, our results show that all the c. jejuni chicken isolates overcome the virulence abilities of the highly virulent ... | 2013 | 24330718 |
| source attribution of human campylobacter isolates by mlst and fla-typing and association of genotypes with quinolone resistance. | campylobacteriosis is the most frequent zoonosis in developed countries and various domestic animals can function as reservoir for the main pathogens campylobacter jejuni and campylobacter coli. in the present study we compared population structures of 730 c. jejuni and c. coli from human cases, 610 chicken, 159 dog, 360 pig and 23 cattle isolates collected between 2001 and 2012 in switzerland. all isolates had been typed with multi locus sequence typing (mlst) and flab-typing and their genotypi ... | 2013 | 24244747 |
| assessment of glycan interactions of clinical and avian isolates of campylobacter jejuni. | campylobacter jejuni strain 11168 was demonstrated to have a broad specificity for eukaryotic surface glycosylation using glycan array analysis. the initial screen indicated that sialic acid and mannose are important binding partners after environmental stress, while galactose and fucose structures are likely to be involved in persistent infection. | 2013 | 24119179 |
| extracellular secretion of protease htra from campylobacter jejuni is highly efficient and independent of its protease activity and flagellum. | the serine protease htra of c. jejuni has been identified as a novel secreted virulence factor which opens cell-to-cell junctions by cleaving e-cadherin. efficient c. jejuni transmigration across polarized human epithelial cells requires the intact flagellum and htra; however, the mechanism of htra secretion into the supernatant is unknown. here we show that htra secretion is highly efficient and does not require its proteolytic activity because the protease-inactive s197a mutant is secreted lik ... | 2013 | 24265934 |