| [evaluation of the immunochromatography kit for detection of emetic-toxin producing bacillus cereus]. | we evaluated the sensitivity and specificity of an immunochromatography kit, single-path emetic tox mrk (merck), which targets a marker protein for the detection of bacillus cereus that produces emetic toxin. strains were isolated after outbreaks of food poisoning, and from retail prepared foods and food products. the strains were examined for the presence of the emetic toxin-synthetase gene by pcr. all 58 emetic strains isolated from the food poisoning cases showed a positive reaction in the im ... | 2012 | 23470854 |
| multifunctional zno/nylon 6 nanofiber mats by an electrospinning-electrospraying hybrid process for use in protective applications. | zno/nylon 6 nanofiber mats were prepared by an electrospinning-electrospraying hybrid process in which zno nanoparticles were dispersed on the surface of nylon 6 nanofibers without becoming completely embedded. the prepared zno/nylon 6 nanofiber mats were evaluated for their abilities to kill bacteria or inhibit their growth and to catalytically detoxify chemicals. results showed that these zno/nylon 6 nanofiber mats had excellent antibacterial efficiency (99.99%) against both the gram-negative ... | 2011 | 27877442 |
| antibacterial activity of momordica charantia (curcubitaceae) extracts and fractions. | momordica charantia l. belongs to the family curcubitaceae and it is very common in many brazilian regions. the plant is a liana with flowers and yellow fruits that present red seeds when are ripe. popularly known as "melão-de-sãocaetano", "melão amargo" or "cabaço-amargo", it possesses many uses: antidiabetic, antihelmintic, antmicrobial, anticancerigenous and antioxidant. the phytochemical prospection of the fresh and dried leaves extracts showed the presence of different classes of secondary ... | 2010 | 24826002 |
| assessment of hydrophobicity and roughness of stainless steel adhered by an isolate of bacillus cereus from a dairy plant. | the interaction between the surface of stainless steel and bacillus cereus was studied in terms of the characteristics of interfacial interaction determined from the measurement of the contact angle of the surface of b. cereus and stainless steel in the presence or absence of b. cereus adherence. the microtopographies and the roughness of the surface of stainless steel and stainless steel adhered by b. cereus were evaluated with the help of atomic force microscopy and perfilometry. the strain of ... | 2010 | 24031578 |
| behavioural pattern of vegetative cells and spores of bacillus cereus as affected by time-temperature combinations used in processing of indian traditional foods. | this study was undertaken to evaluate the behaviour of vegetative cells and spores of four potent native toxigenic food isolates of bacillus cereus as affected by selected time-temperature combinations used in processing of indian traditional foods. the vegetative cells of b. cereus when subjected to sublethal heat treatments, individually, in different heating menstra showed a sigmoidal inactivation pattern, with d-values in the range of 3.45 min at 60 °c to 10.6 min at 56 °c in saline. accordi ... | 2010 | 23572684 |
| biochemical and molecular characterization of thermo-alkali tolerant xylanase producing bacteria from thermal springs of manikaran. | one hundred ten alkalo-tolerant thermophilic bacteria were isolated from 17 samples (water and sediment) collected from manikaran. of 110 isolates, 70 showed the production of xylanases and were further screened for growth and production of xylanases at different temperature ranging from 40 to 75°c. eleven isolates that showed growth and xylanase production at temperatures ≥50°c were selected for quantitative estimation in modified reese mineral liquid medium containing wheat bran. maximum xylan ... | 2010 | 22815564 |
| feather degradation by strains of bacillus isolated from decomposing feathers. | feather waste is generated in large amounts as a by-product of commercial poultry processing. this residue is almost pure keratin, which is not easily degradable by common proteolytic enzymes. eight strains of bacillus, isolated from decomposing feathers were tested for the hydrolysis of feather wastes in the laboratory. among these strains, bacillus cereus kb043 was the best feather degrading organism when grown on basal medium containing 1% hen feather as sole source of carbon and nitrogen. it ... | 2010 | 24031481 |
| antimicrobial activity of enterococcus faecium fair-e 198 against gram-positive pathogens. | this study investigated the antimicrobial activity of enterococcus faecium fair-e 198 against bacillus cereus, listeria monocytogenes and staphylococcus aureus. using the critical-dilution method, the bacteriocin produced by e. faecium fair-e 198 inhibited all l. monocytogenes strains evaluated (1,600 to 19,200 au ml(-1)). however, none of the b. cereus and s. aureus strains investigated were inhibited. the maximum activity of this bacteriocin (800 au ml(-1)) was observed in mrs broth, while the ... | 2010 | 24031466 |
| molecular dynamics simulations of bczbp, a deacetylase from bacillus cereus: active site loops determine substrate accessibility and specificity. | bczbp is an lmbe-like, homohexameric, zinc-dependent deacetylase from the opportunistic pathogen bacillus cereus with three, thus far uncharacterized, homologues in b. anthracis. although its specific substrate is still unknown, the enzyme has been shown to preferentially deacetylate n-acetylglucosamine and diacetylchitobiose via an active site based on a zinc-binding motif of the type hxddxnh. in the crystal structure, the active site is located at a deep and partially blocked cleft formed at t ... | 2009 | 26602510 |
| comparative activities of cattle and swine platelet microbicidal proteins. | the bactericidal activities of cattle and swine platelet microbicidal proteins (pmps) with their comparison with human pmp were studied. activities of pmp were tested against bacillus subtilis, bacillus cereus, staphylococcus aureus, staphylococcus epidermidis, micrococcus lysodeikticus and escherichia coli. b. subtilis and b. cereus were high susceptible to pmp at very low concentrations. of the gram-positive cocci studied, m. lysodeikticus and s. aureus were the most, and s. epidermidis the le ... | 2009 | 26783170 |
| simultaneous detection of pathogenic b. cereus, s. aureus and l. monocytogenes by multiplex pcr. | three important foodborne pathogens, bacillus cereus, listeria monocytogenes and staphylococcus aureus are of major concern for food safety in terms of frequency and seriousness of the disease. the occurrence these three important pathogens and their coexistence in food matrices are predominant. moreover, symptoms associated with b. cereus and s. aureus food poisoning not only closely resembles each other but can also be overlapping with other foodborne infections. in this context, detection of ... | 2009 | 23100783 |
| pharmacognostic, phytochemical and anti-microbial studies of solanum indicum leaves. | the leaves of solanum indicum showed the presence of stellate trichome. ethanolic extract of leaves solanum indicum showed antibacterial activity against staphylococcus aureus, bacillus cereus, escherichia coli where as chloroform extract, acetone extract and ethanol showed anti bacterial against pseudomonas. the aqueous extract did not show any anti microbial activity towards the tested organism. none of the extract showed anti fungal activity. | 2009 | 22557334 |
| family portrait of bacillus cereus and bacillus weihenstephanensis cereulide-producing strains. | two thousand bacillus cereus sensu lato isolates from food and environmental matrices were screened by pcr for the presence of cereulide-producing strains. this survey identified 73 potential emetic strains, most of which originated from non-random food and clinical samplings. none of the 460 bacillus thuringiensis, bacillus mycoides and bacillus pseudomycoides strains were pcr-positive for the cereulide genetic determinants. the chromosomal and extrachromosomal gene pool diversity of a subset o ... | 2009 | 23765791 |
| the science of salsa: antimicrobial properties of salsa components to learn scientific methodology. | most ethnic foods and cooking practices have incorporated the use of spices and other food additives. many common spices have crossed cultural boundaries and appear in multiple ethnic cuisines. recent studies have demonstrated that many of these ingredients possess antimicrobial properties against common food spoilage microorganisms. we developed a laboratory exercise that promotes the use of scientific methodology to evaluate the effectiveness of salsa components at inhibiting the growth of und ... | 2009 | 23653684 |
| in vitro evaluation of datura innoxia (thorn-apple) for potential antibacterial activity. | various parts of datura innoxia were examined for potential antibacterial activity by preparing their crude aqueous and organic extracts against gram-negative bacteria (escherichia coli and salmonella typhi) and gram-positive bacteria (bacillus cereus, bacillus subtilis and staphylococcus aureus). the results of agar well diffusion assay indicated that the pattern of inhibition depends largely upon the plant part, solvent used for extraction and the organism tested. extracts prepared from leaves ... | 2008 | 23100734 |
| antimicrobial activity of various cationic molecules on foodborne pathogens. | antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, gram-positive and gram-negative bacteria. the minimum inhibitory concentration (mic) and the minimum bactericidal concentration (mbc) of α-poly-l-lysine (poly-lys), α-poly-l-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on bacillus subtilis, ... | 2007 | 27517822 |
| risk factors for post-traumatic endophthalmitis in patients with positive intraocular cultures. | to determine the risk factors for post-traumatic endophthalmitis in patients with positive intraocular culture. | 2017 | 28221562 |
| an outbreak of foodborne diarrheal illness among soldiers in mina during hajj: the role of consumer food handling behaviors. | an investigation of the outbreak was initiated as a result of the number of cases of gastroenteritis reporting to a general hospital in mina during the pilgrimage to makkah (hajj). this study was conducted to identify the source of the outbreak, assess its extent, and make recommendations to prevent similar outbreaks in the future. | 2007 | 23012141 |
| phospholipase c from two bacterial strains acts differently on pure phospholipids and membrane bound glycosylphosphatidylinositol (gpi) anchors. | phospholipase c (plc) was purified to homogeneity from the culture filtrate of bacillus cereus (65-fold, 540 u/mg protein) and b. thuringiensis (76-fold, 306 u/mg protein) by conventional techniques of enzyme purification. the purified enzymes have the molecular mass of 34 kda and 38 kda respectively, as determined by sds-page. both the plcs exhibited identical sensitivity to ph, temperature, cations, anions and inhibitors like glutathione and p-chloromercuribenzoate. plc-bc showed a preference ... | 2005 | 23923568 |
| phytochemical and antimicrobial studies of extracts of solanum xanthocarpum. | antibacterial activity of various parts (stem, leaf and fruits) of solvent extracts (petroleum ether, alcohol and acetone) of solanum xanthocarpum against escherichia coil, klebsiella of solanum xanthocarpum showed high sensitivity to escherichia coil & less sensitivity and resistance to bacillus cereus. in control, no inhibitory zone was observed. | 2004 | 22557158 |
| antimicrobial potency of the leaf - stalk extract of curcuma longa (linn). | the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ... | 2000 | 22557005 |
| antimicrobial action of the leaf extract of jatropha tanjorensis (e&r). | the ethalonic extract of the leaves of jatropha tanjorensis ellis & saroja was tested for antimicrobial activities against gram positive - bacillus cereus, bacillus subtilis, staphylococcus aureus, gram negative e. coli and mycobacterium pheli. | 1998 | 22556869 |
| competition as a source of errors in rapd analysis. | we have used artificial 1∶1 dna mixtures of all pairwise combinations of four doubled haploid brassica napus lines to test the ability of rapds to function as reliable dominant genetic markers. in situations where a specific rapd band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1∶1 dna mixture. in 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the rapd reaction. thus, rapd mark ... | 1996 | 24162529 |
| kinetic study of the active site structure of β-amylase from bacillus cereus var. mycoides. | the subsite affinities of the active site of β-amylase from bacillus cereus var. mycoides were evaluated based on hiromi's theory, using (14)c-radiolabeled maltooligosaccharides as substrate. it was estimated that the active site consisted of six subsites, and all subsite affinities could be evaluated. the active site had a common subsite arrangement with those of β -amylases from soybean and wheat bran. the intrinsic breakdown rate constant of α-1,4 glucosidic linkage (kint) was five to seven t ... | 1996 | 27281142 |
| distribution of toxigenic bacillus cereus in rice samples marketed in hong kong. | eleven of 16 samples of rice on sale in rice shops and supermarkets in hong kong contained bacillus cereus. although b. cereus counts did not exceed 100 bacteria/g in most of the positive samples, a sample of thai red rice and a poor quality rice originating from china contained between 300 to 1000 cells/g and 10(4) to 2×10(5) cells/g, respectively. nine strains produced an enterotoxin responsible for the diarrhoeal-type b. cereus food poisoning and seven of these strains also produced a haemoly ... | 1995 | 24415029 |
| antimicrobial action of the leaf extract of moringa oleifera lam. | the ethnolic extract of the leaves of moringa oleifera lam. (fam. moringaceae) was tested for antimicrobial activities against gram positive - bacillus cereus, bacillus subtilis staphylococcus aureus, sarcina lutea: gram negative - escherichia coli and acid fast mycobacterium phlei. significant antimicrobial activity of the extract was found in this study. | 1995 | 22556699 |
| ph and acidity in lactic-fermenting cereal gruels: effects on viability of enteropathogenic microorganisms. | survival of bacillus cereus, campylobacter jejuni, enterotoxigenic escherichia coli, salmonella typhimurium and shigella flexneri during lactic acid fermentation of cereal gruels prepared from low-tannin (white) and high-tannin (red) sorghum varieties was studied. a previously fermented gruel (starter culture, sc) recycled daily or stored for 7, 14 or 28 days, germinated cereal flour (power flour, pf), or a combination of pf and sc (pf+sc) were used as starters. at 24 h, the ph of all gruels wit ... | 1994 | 24421191 |
| inhibition of bacillus spores by combinations of heat, potassium sorbate, nacl and ph. | viability of spores of bacillus cereus was totally inhibited at 85°c over 30 min by adding 0.4% (w/v) potassium sorbate with 6% (w/v) nacl at ph 4.5. viability of b. stearothermophilus spores was totally inhibited at 95°c for 45 min in a buffer at ph 4.2 containing 0.8% (w/v) potassium sorbate and 8% (w/v) nacl. a synergistic inhibitory effect was demonstrated in some of the combinations. the inhibition may be due to interference with the heat-resistance apparatus of the spores. | 1994 | 24421139 |
| effect of apigeninidin on the growth of selected bacteria. | apigeninidin, a 3-deoxyanthocyanidin present in sorghum, was chemically synthesized and effectively purified by extracting impurities from aqueous 2 n hc1 into ethyl acetate. apigeninidin (50μmol) applied to filter paper disks inhibited the growth on agar plates of selected gram-positive bacteria (bacillus cereus, staphylococcus aureus, staphylococcus epidermidis, andstreptococcus faecalis) and to a lesser extent gram-negative bacteria (escherichia coli, serratia marcens, andshigella flexneri). ... | 1993 | 24249081 |
| inhibited growth of common enteropathogenic bacteria in lactic-fermented cereal gruels. | a natural lactic fermentation of mixtures of water and whole flour of either maize or high-tannin sorghum was obtained either before or after cooking to a weaning gruel: the preparations had a final ph of about 3.8 (range 3.67 to 4.00) and a ratio of lactic acid to acetic acid of 9∶1 (w/w). the growth of added (about 10(7) c.f.u./g gruel) gram-negative intestinal pathogenic bacteria, enterotoxigenicescherichia coli, campylobacter jejuni, shigella flexneri andsalmonella typhimurium, was strongly ... | 1992 | 24425608 |
| interactions of bacteria and microflagellates in sequencing batch reactors exhibiting enhanced mineralization of toxic organic chemicals. | community level interactions were studied in non-axenic sequencing batch reactors (sbrs) being used to treat 2,4-dinitrophenol (dnp). increasing the influent dnp concentrations from 1 to 10 µg ml(-1) eliminated large predatory organisms such as rotifers and ciliated protozoa from the sbrs. under steady-state conditions at a dnp concentration of 10 µg ml(-1), supplemental additions of glucose enhanced dnp degradation and led to the establishment of a microbial community consisting of five species ... | 1992 | 24192859 |
| a microbiological assay for sterigmatocystin, t-2 toxin and zearalenone. | a survey was done to find microorganisms useful for assaying sterigmatocystin; t-2 toxin and zearalenone.staphylococcus aureus was found to be sensitive to t-2 toxin and zearalenone;bacillus cereus was found to be sensitive to t-2 toxin only; andescherichia coli was sensitive to sterigmatocystin. the response of the organisms to sterigmatocystin; t-2 toxin and zearalenone was found to be linear between 4 and 100 μg with sterigmatocystin toe. coli; between 2 and 25 μg with t-2 toxin tostaph, aure ... | 1989 | 23605231 |
| a new bioassay system for screening high berberine-producing cell colonies of thalictrum minus. | a new method of estimating the amount of berberine released from minute cell colonies of thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. in this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and the concentration of berberine which has been released from the cells into the agar piece is assayed by the antibacterial activity against bacillus cereus mt2026. screening of 1000 ... | 1987 | 24248650 |
| development of intestinal microflora and occurrence of diarrhoea in sucking foals: effects of bacillus cereus var. toyoi supplementation. | almost all foals develop transient diarrhoea within the first weeks of life. studies indicated different viral, bacterial, and parasitic causes, such as rotavirus, clostridium perfringens, escherichia coli, and cryptosporidium are discussed. but little is known about the development of intestinal microflora in foals. the present study investigated whether the supplementation with bacillus cereus var. toyoi would modify the developing intestinal microflora and consequently reduce diarrhoea in foa ... | 2015 | 25889817 |
| complete genome sequence of bacillus toyonensis bct-7112t, the active ingredient of the feed additive preparation toyocerin. | strain bct-7112, previously identified as bacillus cereus var. toyoi, is the type strain of the species bacillus toyonensis, a novel species of the b. cereus group. the complete genome of this strain, which is the active ingredient of the feed additive preparation toyocerin, has been sequenced and annotated to reveal the genetic properties of this probiotic organism with a long history of safe use in animal nutrition. | 2013 | 24356843 |
| description of bacillus toyonensis sp. nov., a novel species of the bacillus cereus group, and pairwise genome comparisons of the species of the group by means of ani calculations. | strain bct-7112(t) was isolated in 1966 in japan from a survey designed to obtain naturally occurring microorganisms as pure cultures in the laboratory for use as probiotics in animal nutrition. this strain, which was primarily identified as bacillus cereus var toyoi, has been in use for more than 30 years as the active ingredient of the preparation toyocerin(®), an additive for use in animal nutrition (e.g. swine, poultry, cattle, rabbits and aquaculture). despite the fact that the strain was i ... | 2013 | 23791203 |
| bacillus cereus var. toyoi promotes growth, affects the histological organization and microbiota of the intestinal mucosa in rainbow trout fingerlings. | in this preliminary study, we evaluated the effects of a gram-positive soil bacteria bacillus cereus var. toyoi on the growth performance, digestive enzyme activities, intestinal morphology, and microbiota in rainbow trout oncorhynchus mykiss fingerlings. trout were maintained in a recirculation system and fed 2 diets: 1) a commercial trout feed deprived of the probiotic and 2) the same diet but with the spores of the probiotic bacteria dissolved in fish oil during the manufacturing of the feed ... | 2013 | 23508031 |
| production of polyhydroxyalkanoates (pha) using sludge from different wastewater treatment processes and the potential for medical and pharmaceutical applications. | in this study, seven strains of bacteria with polyhydroxyalkanoates (pha)-producing ability (i.e., bacillus cereus, pseudomonas putida, bacillus pumilus, pseudomona-huttiensis, yersinia-frederiksenii, aeromonas ichthiosmia and sphingopyxis terrae) were isolated from various waste treatment plants in hong kong. simultaneous wastewater treatment and pha accumulation were successfully achieved in the bioreactors using isolated bacteria from different sludges. at the organic loading less than 13000 ... | 2017 | 28387154 |
| seasonal variability in size-segregated airborne bacterial particles and their characterization at different source-sites. | size-segregated aerosol samplings were carried out near the potential sources of airborne biological particles i.e. at a landfill site, an agricultural field and a road side restaurant-cluster site in winter, spring and summer seasons during 2013-2015 in new delhi. the culturable airborne bacterial (cab) concentrations showed significant seasonal variation from higher to moderate in spring and winter seasons and lowest during summer. highest cab concentrations were observed at the okhla landfill ... | 2017 | 28390023 |
| diversity of endophytic bacteria in a fern species dryopteris uniformis (makino) makino and evaluation of their antibacterial potential against five foodborne pathogenic bacteria. | the fern plant dryopteris uniformis has traditionally been used in herbal medicine and possesses many biological activities. this study was conducted to explore the endophytic bacterial diversity associated with d. uniformis and evaluate their antibacterial potential against foodborne pathogenic bacteria (fpb). among 51 isolated endophytic bacteria (eb), 26 eb were selected based on their morphological characteristics and identified by 16s rrna gene analysis. the distribution of eb was diverse i ... | 2017 | 28418717 |
| expression and purification of transmembrane protein mere from mercury resistant bacillus cereus. | mercury resistant (hg(r)) bacteria were isolated from heavy metal polluted wastewater and soil collected near to tanneries of district kasur, pakistan. bacterial isolates az-1, az-2 and az-3 showed resistance up to 40 μg/ml against mercuric chloride (hgcl2). 16s rdna ribotyping and phylogenetic analysis were performed for the characterization of selected isolates as bacillus sp. az-1 (kt270477), bacillus cereus az-2 (kt270478) and bacillus cereus az-3 (kt270479). phylogenetic relationship on the ... | 2017 | 28783894 |
| effects of secondary metabolites of permafrost bacillus sp. on cytokine synthesis by human peripheral blood mononuclear cells. | we studied the effects of secondary metabolites of bacillus sp. isolated from late neogene permafrost on secretion of proinflammatory (tnf-α, il-1β, il-8, il-2, and ifnγ) and antiinflammatory (il-4 and il-10) cytokines by human peripheral blood mononuclear cells. it was found that metabolites of bacillus sp. produced more potent effect on cytokine secretion than mitogen phytohemagglutinin and metabolites of bacillus cereus, medicinal strain ip5832. activity of metabolites depended on the tempera ... | 2017 | 28726195 |
| an experimental study of postmortem decomposition of methomyl in blood. | methomyl (s-methyl-1-n-[(methylcarbamoyl)oxy]thioacetimidate) is a carbamate pesticide. it has been noted that in some cases of methomyl poisoning, methomyl is either not detected or detected only in low concentrations in the blood of the victims. however, in such cases, methomyl is detected at higher concentrations in the vitreous humor than in the blood. this indicates that methomyl in the blood is possibly decomposed after death. however, the reasons for this phenomenon have been unclear. we ... | 2017 | 28457508 |
| chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans. | the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ... | 2017 | 28725151 |
| investigation on the antimicrobial activities of gingers (etlingera coccinea (blume) s.sakai & nagam and etlingera sessilanthera r.m.sm.) endemic to borneo. | to investigate the antimicrobial properties of e. coccinea and e. sessilanthera and to isolate and identify the antimicrobial compounds. | 2017 | 28708293 |
| development and characterization of bioactive edible films from spider crab (maja crispata) chitosan incorporated with spirulina extract. | active food packaging films based on crab chitosan and spirulina extract (se) were developed. the effects of the se incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. ftir was carried out to observe the potential modifications of the chitosan films when incorporated with se. the obtained results suggested that incorporation of se into chitosan ... | 2017 | 28705500 |
| antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves. | fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ... | 2017 | 28698913 |
| variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons. | polyhydroxyalkanoates (pha) are efficient, renewable and environment friendly polymeric esters. these polymers are synthesized by a variety of microbes under stress conditions. this study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. six bacterial strains were isolated and identified as bacillus cereus (kf270349), klebsiella pneumoniae (kf270350), bacillus subtilis (kf270351), brevibacterium halotolerance (kf270352) ... | 2017 | 28629970 |
| synthesis of new thiazolyl coupled pyrazoles bearing 2,4-thiazolidinedionyl pharmacophore and their anti-inflammatory and antibacterial evaluation. | 2, 4-thiazolidinedione (tzd), pyrazole and thiazole heterocyclic rings exhibit wide range of pharmacological activities. medicinal chemists use these heterocyclic moieties as scaffolds in drug designing and discovery. the existing medicaments, celecoxib and meloxicam, used for treating inflammation and pain are having, pyrazole and thiazole, respectively as a key scaffolds. pyrazoles coupled with 2, 4-thiazolidinediones may display enhanced anti inflammatory activity. with this hope the title wo ... | 2017 | 28618987 |
| in vitro anti-oxidant and anti-microbial potentiality investigation of different fractions of caryota urens leaves. | caryota urens is a member of the arecaceae family and a common plant in the southeast asian region. this plant has been reported as an anti-microbial agent in recent years. thus, we aimed to find out the mic (minimum inhibitory concentration) against different pathogenic microorganism. | 2016 | 28536384 |
| effect of microorganisms on reducing cadmium uptake and toxicity in rice (oryza sativa l.). | this study analyzed the application of three microorganism inoculums, including bacillus subtilis, bacillus cereus, and commercial effective microorganism (em) solution in order to determine cadmium (cd) reduction in rice (oryza sativa l.) and rice growth promotion. rice was grown in cd-contaminated soil (120 mg/kg) and selected microorganisms were inoculated. cd concentration and rice weight were measured at 45 and 120 days of the experiment. the result showed that b. subtilis inoculation into ... | 2017 | 28480489 |
| isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india. | a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ... | 2017 | 28720951 |
| pcr denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in haemaphysalis flava ticks. | ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. in the present study, the intestinal bacterial flora associated with haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (pcr) and denaturing gradient gel electrophoresis (dgge). eleven distinct dgge bands were found using pcr-dgge method. sequence ... | 2017 | 28426403 |
| sensitive detection of staphylococcus aureus with vancomycin-conjugated magnetic beads as enrichment carriers combined with flow cytometry. | a novel sandwich strategy was designed to detect staphylococcus aureus. the strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. vancomycin (van), which exerts a strong antibacterial effect on gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. to effectively concentrate s. aureus, we used bovine serum albumin (bsa) as the amplification carrier to modify ... | 2017 | 28590745 |
| production and characterization of a new biodegradable fenugreek seed gum based active nanocomposite film reinforced with nanoclays. | in the present work, fenugreek seed gum (fsg)/clay nanocomposite films were prepared with nanoclays (na(+) montmorillonite [mmt], halloysite [hnt] and nanomer(®) i.44 p [nm]) at different amounts (0, 2.5, 5.0 and 7.5g clay/100g fsg) by solution casting method and characterized. increasing amount of nanoclay significantly (p<0.05) improved oxygen barrier and thermal properties of the biodegradable films. agar diffusion tests revealed that fsg based nanocomposite films exhibited strong antimicrobi ... | 2017 | 28536016 |
| development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens. | bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ... | 2017 | 28505584 |
| antibacterial effects of lactobacillus isolates of curd and human milk origin against food-borne and human pathogens. | this study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific pcr and 16s rrna-based sequencing. these strains belonged to five species, lactobacillus casei, l. delbrueckii, l. fermentum, l. plantarum, and l. pentosus. antibacterial activities of cell-free supernatants (cfss) of all the lactobacillus isolates were estim ... | 2017 | 28401466 |
| persistent anthrax as a major driver of wildlife mortality in a tropical rainforest. | anthrax is a globally important animal disease and zoonosis. despite this, our current knowledge of anthrax ecology is largely limited to arid ecosystems, where outbreaks are most commonly reported. here we show that the dynamics of an anthrax-causing agent, bacillus cereus biovar anthracis, in a tropical rainforest have severe consequences for local wildlife communities. using data and samples collected over three decades, we show that rainforest anthrax is a persistent and widespread cause of ... | 2017 | 28770842 |
| mosquito microbiome dynamics, a background for prevalence and seasonality of west nile virus. | symbiotic microbial communities augment host phenotype, including defense against pathogen carriage and infection. we sampled the microbial communities in 11 adult mosquito host species from six regions in southern ontario, canada over 3 years. of the factors examined, we found that mosquito species was the largest driver of the microbiota, with remarkable phylosymbiosis between host and microbiota. seasonal shifts of the microbiome were consistently repeated over the 3-year period, while region ... | 2017 | 28421042 |
| the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells. | six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ... | 2017 | 28811611 |
| synthesis, antimicrobial, antiquorum-sensing and antitumor activities of new benzimidazole analogs. | new benzimidazole analogs were prepared and tested for antimicrobial efficacy toward escherichia coli atcc 12435, bacillus cereus uw 85, staphylococcus aureus atcc 29213, candida albicans and aspergillus fumigatus 293. results indicated that compound 10 has potent and broad spectrum antimicrobial activity. in addition, 4b and 5c showed eminent antimicrobial efficacy toward b. cereus, s. aureus, c. albicans and a. fumigatus. furthermore, 12 and 14 demonstrated interesting antifungal activity towa ... | 2017 | 28623814 |
| antimicrobial activity and chemical composition of brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. | brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. however, studies on the antimicrobial activity of brunfelsia uniflora flower oleoresin have not been found yet. this study aimed to evaluate the chemical composition and antimicrobial activity of b. uniflora flower oleoresin obtained by supercritical carbon dioxide. oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition ... | 2017 | 28407186 |
| biological characterization of the biocontrol agent bacillus amyloliquefaciens cpa-8: the effect of temperature, ph and water activity on growth, susceptibility to antibiotics and detection of enterotoxic genes. | this work focuses on the biological understanding of the biocontrol agent bacillus amyloliquefaciens cpa-8 in order to accomplish the characterization required in the registration process for the development of a microorganism-based product. the tolerance of cpa-8 to grow under different ph-temperature and water activity (a w)-temperature conditions was widely demonstrated. regarding the ph results, optimum growth at the evaluated conditions was observed at 37 °c and ph between 7 and 5. on the c ... | 2017 | 28664219 |
| contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (arachis hypogaea l.). | the present investigation analyzes the in vitro p solubilization [ca-p, al-p, fe(ii)-p, and fe(iii)-p] efficiency of native psb strains from acid soils of odisha and exploitation of the same through biofertilization in peanut (arachis hypogaea l.) growth and p acquisition. one hundred six numbers of soil samples with ph ≤ 5.50 were collected from five districts of odisha viz., balasore, cuttack, khordha, keonjhar, and mayurbhanj. one bacterial isolate from each district were selected and analyze ... | 2017 | 28455550 |
| structural and immunochemical relatedness suggests a conserved pathogenicity motif for secondary cell wall polysaccharides in bacillus anthracis and infection-associated bacillus cereus. | bacillus anthracis (ba) and human infection-associated bacillus cereus (bc) strains bc g9241 and bc 03bb87 have secondary cell wall polysaccharides (scwps) comprising an aminoglycosyl trisaccharide repeat: →4)-β-d-manpnac-(1→4)-β-d-glcpnac-(1→6)-α-d-glcpnac-(1→, substituted at glcnac residues with both α- and β-galp. in bc g9241 and bc 03bb87, an additional α-galp is attached to o-3 of mannac. using nmr spectroscopy, mass spectrometry and immunochemical methods, we compared these structures to s ... | 2017 | 28832613 |
| draft genome sequences from a novel clade of bacillus cereus sensu lato strains, isolated from the international space station. | the draft genome sequences of six bacillus strains, isolated from the international space station and belonging to the bacillus anthracis-b. cereus-b. thuringiensis group, are presented here. these strains were isolated from the japanese experiment module (one strain), u.s. harmony node 2 (three strains), and russian segment zvezda module (two strains). | 2017 | 28798168 |
| comparative genomics of bacillus thuringiensis reveals a path to specialized exploitation of multiple invertebrate hosts. | understanding the genetic basis of host shifts is a key genomic question for pathogen and parasite biology. the bacillus cereus group, which encompasses bacillus thuringiensis and bacillus anthracis, contains pathogens that can infect insects, nematodes, and vertebrates. since the target range of the essential virulence factors (cry toxins) and many isolates is well known, this group presents a powerful system for investigating how pathogens can diversify and adapt to phylogenetically distant ho ... | 2017 | 28790205 |
| non-toxin-producing bacillus cereus strains belonging to the b. anthracis clade isolated from the international space station. | in an ongoing microbial observatory investigation of the international space station (iss), 11 bacillus strains (2 from the kibo japanese experimental module, 4 from the u.s. segment, and 5 from the russian module) were isolated and their whole genomes were sequenced. a comparative analysis of the 16s rrna gene sequences of these isolates showed the highest similarity (>99%) to the bacillus anthracis-b. cereus-b. thuringiensis group. the fatty acid composition, polar lipid profile, peptidoglycan ... | 2017 | 28680972 |
| milk-originated bacillus cereus sensu lato strains harbouring bacillus anthracis-like plasmids are genetically and phenotypically diverse. | bacillus cereus sensu lato is widely distributed in food products, including raw and processed milk. plasmids often determine bacterial virulence and toxicity, but their role in the evolution of b. cereus sensu lato is only partly known. here, we observed that nearly 8% of b. cereus sensu lato isolates were positive for pxo1-like plasmids and 12% for pxo2-like plasmids in raw and ultra-heat-treated (uht) milk from one dairy plant. however, pxo1-like plasmids were significantly more frequent in r ... | 2017 | 28648290 |
| in defense of bacillus thuringiensis, the safest and most successful microbial insecticide available to humanity - a response to efsa. | the bacillus cereus group contains vertebrate pathogens such as bacillus anthracis and bacillus cereus and the invertebrate pathogen bacillus thuringiensis. microbial biopesticides based on b. thuringiensis (bt) are widely recognized as being among the safest and least environmentally damaging insecticidal products available. nevertheless, a recent food poisoning incident prompted a european food safety authority review which argued that b. thuringiensis poses a health risk equivalent to b. cere ... | 2017 | 28645183 |
| assembly and function of the bacillus anthracis s-layer. | bacillus anthracis, the anthrax agent, is a member of the bacillus cereus sensu lato group, which includes invasive pathogens of mammals or insects as well as nonpathogenic environmental strains. the genes for anthrax pathogenesis are located on two large virulence plasmids. similar virulence plasmids have been acquired by other b. cereus strains and enable the pathogenesis of anthrax-like diseases. among the virulence factors of b. anthracis is the s-layer-associated protein bsla, which endows ... | 2017 | 28622090 |
| recombinant protein expression, crystallization, and biophysical studies of a bacillus-conserved nucleotide pyrophosphorylase, bcmazg. | to overcome safety restrictions and regulations when studying genes and proteins from true pathogens, their homologues can be studied. bacillus anthracis is an obligate pathogen that causes fatal inhalational anthrax. bacillus cereus is considered a useful model for studying b. anthracis due to its close evolutionary relationship. the gene cluster ba1554 - ba1558 of b. anthracis is highly conserved with the bc1531- bc1535 cluster in b. cereus, as well as with the bt1364-bt1368 cluster in bacillu ... | 2017 | 28570540 |
| draft genome sequences of bacillus cereus e41 and bacillus anthracis f34 isolated from algerian salt lakes. | two strains of bacillus, b. cereus e41 and b. anthracis f34, were isolated from a salt lake in aïn m'lila-oum el bouaghi, eastern algeria, and ain baida-ouargla, southern algeria, respectively. their genomes display genes for the production of several bioactive secondary metabolites, including polyhydroxyalkanoate, iron siderophores, lipopeptides, and bacteriocins. | 2017 | 28522726 |
| draft genome sequence of bacillus cereus la2007, a human-pathogenic isolate harboring anthrax-like plasmids. | we present the genome sequence of bacillus cereus la2007, a strain isolated in 2007 from a fatal pneumonia case in louisiana. sequence-based genome analysis revealed that la2007 carries a plasmid highly similar to bacillus anthracis pxo1, including the genes responsible for the production and regulation of anthrax toxin. | 2017 | 28428293 |
| complete genome sequence of the nematicidal bacillus thuringiensis mybt18246. | 10.1601/nm.5000 is a rod-shaped facultative anaerobic spore forming bacterium of the genus 10.1601/nm.4857. the defining feature of the species is the ability to produce parasporal crystal inclusion bodies, consisting of δ-endotoxins, encoded by cry-genes. here we present the complete annotated genome sequence of the nematicidal 10.1601/nm.5000 strain mybt18246. the genome comprises one 5,867,749 bp chromosome and 11 plasmids which vary in size from 6330 bp to 150,790 bp. the chromosome contains ... | 2017 | 28852435 |
| levels of l-malate and other low molecular weight metabolites in spores of bacillus species and clostridium difficile. | dormant spores of bacillus species lack atp and nadh and contain notable levels of only a few other common low mol wt energy reserves, including 3-phosphoglyceric acid (3pga), and glutamic acid. recently, bacillus subtilis spores were reported to contain ~ 30 μmol of l-malate/g dry wt, which also could serve as an energy reserve. in present work, l-malate levels were determined in the core of dormant spores of b. subtilis, bacillus cereus, bacillus megaterium and clostridium difficile, using bot ... | 2017 | 28850573 |
| antimicrobial and antioxidant activities of the extract and fractions of tetradenia riparia (hochst.) codd (lamiaceae) leaves from brazil. | tetradenia riparia (lamiaceae) is native to central africa popularly known as myrrh, used in folk medicine to treat various diseases like malaria, gastroenteritis, and tropical skin disease. this research was to evaluate the antioxidant and antibacterial activities of the crude extract (ce) and fractions (fr) of the t. riparia by classical chromatography. the ce of t. riparia leaves was submitted to column chromatographic fractionation to obtain four fractions of the interest, which were identif ... | 2017 | 28840299 |
| biological activity of the non-microbial fraction of kefir: antagonism against intestinal pathogens. | kefir is a fermented milk obtained by the activity of kefir grains which are composed of lactic and acetic acid bacteria, and yeasts. many beneficial health effects have been associated with kefir consumption such as stimulation of the immune system and inhibition of pathogenic microorganisms. the biological activity of kefir may be attributed to the presence of a complex microbiota as well as the microbial metabolites that are released during fermentation. the aim of this work was to characteri ... | 2017 | 28831964 |
| discovery of a potent inhibitor class with high selectivity toward clostridial collagenases. | secreted virulence factors like bacterial collagenases are conceptually attractive targets for fighting microbial infections. however, previous attempts to develop potent compounds against these metalloproteases failed to achieve selectivity against human matrix metalloproteinases (mmps). using a surface plasmon resonance-based screening complemented with enzyme inhibition assays, we discovered an n-aryl mercaptoacetamide-based inhibitor scaffold that showed sub-micromolar affinities toward coll ... | 2017 | 28820255 |
| synergistic antibacterial and antibiofilm efficacy of nisin in combination with p-coumaric acid against foodborne bacteria bacillus cereus and salmonella typhimurium. | the aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria bacillus cereus and salmonella typhimurium. their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both t ... | 2017 | 28815637 |
| simultaneous removal of nitrogen and phosphorous from domestic wastewater using bacillus cereus gs-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal. | a newly isolated gs-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal was identified as bacillus cereus gs-5 based on its phenotypic and phylogenetic characteristics. the isolate had exhibited efficient nh4(+)-n, no3(-)-n, no2(-)-n and po4(3-)-p removal from nutrient spiked real domestic wastewater with average rates of 2.62, 2.69, 1.16 and 0.42mgl(-1)h(-1), respectively under aerobic condition. metabolic inhibitor based mass balance an ... | 2017 | 28803098 |
| growth of amanita caesarea in the presence of pseudomonas fluorescens and bacillus cereus. | the ectomycorrhizal (ecm) fungus amanita caesarea cect 20127 was tested in vitro with two potentially mycorrhizal-promoting bacterial strains, pseudomonas fluorescens cect 844 and bacillus cereus cect 148. although p. fluorescens showed spatial and temporal compatibility with a. caesarea, it did not affect growth of the fungus. conversely, b. cereus exhibited no such compatibility and also inhibited fungal growth. the expression pattern of the a. caesarea gene acmst-1 was analysed using real-tim ... | 2017 | 28800852 |
| methionine residues in exoproteins and their recycling by methionine sulfoxide reductase ab serve as an antioxidant strategy in bacillus cereus. | during aerobic respiratory growth, bacillus cereus is exposed to continuously reactive oxidant, produced by partially reduced forms of molecular oxygen, known as reactive oxygen species (ros). the sulfur-containing amino acid, methionine (met), is particularly susceptible to ros. the major oxidation products, methionine sulfoxides, can be readily repaired by methionine sulfoxide reductases, which reduce methionine sulfoxides [met(o)] back to methionine. here, we show that methionine sulfoxide re ... | 2017 | 28798727 |
| potassium-ion-selective fluorescent sensors to detect cereulide, the emetic toxin of b. cereus, in food samples and hela cells. | we report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. for this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. the fluorescence detection of natural cereulide in rice samples was achieved by using ... | 2017 | 28794952 |
| proposal of nine novel species of the bacillus cereus group. | nine novel gram-stain-positive bacteria were investigated by a polyphasic taxonomic approach. based on the analysis of 16s rrna gene sequences, these strains belonged to the bacillus cereus group, sharing over 97 % similarity with the known species of this group, and less than 95 % similarity with other species of the genus bacillus. multilocus sequence typing analysis showed that they formed nine robust and well-separated branches from the known species. the digital dna-dna hybridization (dddh) ... | 2017 | 28792367 |
| novel motb as a potential predictive tool for identification of b. cereus, b. thuringiensis and differentiation from other bacillus species by triplex real-time pcr. | quantitative triplex real-time pcr (qpcr) offers an alternative method for detection of bacterial contamination. it provides quantitation of the number of gene copies. in our study, we established a qpcr assay to detect and quantify the specificity towards bacillus cereus and b. thuringiensis. the assay was designed to detect a 280 bp fragment of motb gene encoding the flagellar motor protein, specific for detection of b. cereus and b. thuringiensis, excluding other group species b. pseudomycoid ... | 2017 | 28778821 |
| gene expression and in silico analysis of snakehead murrel interleukin 8 and antimicrobial activity of c-terminal derived peptide ws12. | chemokines have been known for their wide range of functions including chemoattractant property in humans and other vertebrate organisms. they act as a bridge between innate and adaptive immune system. in the present study, we have identified a cxc chemokine from the cdna library of c. striatus; on the basis of orthology study, it was found highly identical to interleukin 8 (il8). the bioinformatics analysis of the chemokine revealed the presence of a typical γ-core domain and a cxc motif at the ... | 2017 | 28778316 |
| antimicrobial activity of endophytic bacterial populations isolated from medical plants of iran. | endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. the aim of this study was molecular identification and measuring the antimicrobial activity of endophytic actinomycetes isolated from medicinal plants of iran. | 2017 | 28775818 |
| bacillus cereus group bacteriophage flapjack genome sequence. | the bacillus cereus group bacteriophage flapjack, a double-stranded dna (dsdna) myoviridae isolate collected from soil collected in washington, dc, is a member of cluster c3 and encodes an intramolecular chaperone-containing tail fiber protein previously found in podoviridae and siphoviridae but not annotated in the myoviridae. | 2017 | 28774974 |
| pan-genome and phylogeny of bacillus cereus sensu lato. | bacillus cereus sensu lato (s. l.) is an ecologically diverse bacterial group of medical and agricultural significance. in this study, i use publicly available genomes and novel bioinformatic workflows to characterize the b. cereus s. l. pan-genome and perform the largest phylogenetic and population genetic analyses of this group to date in terms of the number of genes and taxa included. with these fundamental data in hand, i identify genes associated with particular phenotypic traits (i.e., "pa ... | 2017 | 28768476 |
| phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects. | peanut skin (ps) and meal from dry-blanched peanuts (mdbp) were evaluated as sources of phenolic compounds. ps rendered the highest total phenolic content, antioxidant capacity towards abts radical cation, dpph and hydroxyl radicals as well as reducing power. phenolic acids were present in ps and mdbp whereas proanthocyanidins and monomeric flavonoids were found only in ps as identified by hplc-dad-esi-ms(n). procyanidin-rich extracts prevented oxidation in non-irradiated and gamma-irradiated fi ... | 2017 | 28764032 |
| purification and characterization of trypsin produced by gut bacteria from anticarsia gemmatalis. | purification of active trypsin in the digestive process of insects is essential for the development of potent protease inhibitors (pis) as an emerging pest control technology and research into insect adaptations to dietary pis. an important aspect is the presence of proteolytic microorganisms, which contribute to host nutrition. here, we purified trypsins produced by bacteria bacillus cereus, enterococcus mundtii, enterococcus gallinarum, and staphylococcus xylosus isolated from the midgut of an ... | 2017 | 28762531 |
| formulation of menthol-loaded nanostructured lipid carriers to enhance its antimicrobial activity for food preservation. | purpose: due to the antimicrobial property, menthol have significant potential for food preservation and foodstuff shelf life improvement. nevertheless, menthol instability, insolubility, and rapid crystallization in aqueous media make it unsuitable for used in food products. this work was aimed to prepare menthol-loaded nanostructured lipid carriers (nlcs) to enhance its antimicrobial activity. methods: morphology, particle size and size distribution, encapsulation efficiency percent (ee%), and ... | 2017 | 28761828 |
| multiplex pcr coupled with propidium monoazide for the detection of viable cronobacter sakazakii, bacillus cereus, and salmonella spp. in milk and milk products. | cronobacter sakazakii, bacillus cereus, and salmonella spp. are common food-borne pathogens. the aim of this study was to develop a sensitive, specific, and rapid method for the simultaneous detection of these 3 pathogens in milk and milk products. three specific primers were designed based on ompa, inva, and cesb of c. sakazakii, salmonella spp. and b. cereus, respectively, for use in a multiplex pcr (mpcr). to eliminate false-positive results, cells were pretreated with propidium monoazide (pm ... | 2017 | 28755939 |
| cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects. | oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. in this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of bacillus cereus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, salmonella enterica, serratia mar ... | 2017 | 28755871 |
| quantitative prevalence, phenotypic and genotypic characteristics of bacillus cereus isolated from retail infant foods in china. | bacillus cereus is an important foodborne pathogen, which can cause severe food poisoning. the aim of this study was (i) to evaluate the quantitative prevalence of b. cereus in retail prepackaged infant formula and ready-to-eat rice flour in china and (ii) to gain the basic information on pheno- and genotypic characteristics of b. cereus isolates. we found that 40 out of the 587 samples were positive for b. cereus. b. cereus in 3.5% of infant formula samples and 1.0% of rice flour samples outnum ... | 2017 | 28753035 |
| bam35 tectivirus intraviral interaction map unveils new function and localization of phage orfan proteins. | tectiviridae comprises a group of tail-less, icosahedral, membrane-containing bacteriophages that can be divided into two groups by their hosts, either gram-negative or gram-positive bacteria. while the first group is composed of prd1 and nearly identical well characterized lytic viruses, the second one includes more variable temperate phages, like gil16 or bam35, whose hosts are bacillus cereus and related gram-positive bacteria.in the genome of bam35, nearly half of the 32 annotated open readi ... | 2017 | 28747494 |
| antibacterial activity of fusion from biosynthesized acidocin/silver nanoparticles and its application for eggshell decontamination. | bacteriocins from lactic acid bacteria (lab) are useful to control the persistent development of pathogenic microorganisms in food and medicine fields. the bacteriocin acidocin was extracted from lactobacillus acidophilus m1 that was isolated from fermented milk, purified using ammonium sulphate fractionation, and gel filtration column chromatography using sephadex matrix and applied as a potential antibacterial agent. the molecular weight of the purified acidocin was estimated using sds-page to ... | 2017 | 28736866 |
| crystal structures of an atypical aldehyde dehydrogenase having bidirectional oxidizing and reducing activities. | aldehyde dehydrogenases (aldhs) are nad(p)(+)-dependent oxidoreductases that catalyze the oxidation of a variety of aldehydes to their acid forms. in this study, we determined the crystal structures of aldh from bacillus cereus (bcaldh), alone, and in complex with nad(+) and nadp(+). this enzyme can oxidize all-trans-retinal to all-trans-retinoic acid using either nad(+) or nadp(+) with equal efficiency, and atypically, as a minor activity, can reduce all-trans-retinal to all-trans-retinol using ... | 2017 | 28732729 |
| protease hydrolysates of filefish (thamnaconus modestus) byproducts effectively inhibit foodborne pathogens. | in this study, novel antimicrobial peptides had been derived by enzymatic hydrolysis of filefish (thamnaconus modestus) byproduct (hfbp). different proteases, (papain [p], trypsin [t], neutrase [n], pepsin [pe], and the mixture i [pn] and mixture ii [pt]) and different hydrolysis time (60, 120, 180, and 240 min), have been used to generate peptides with different lengths and amino acid sequences. the antimicrobial properties of hfbps were tested, against gram-positive and gram-negative strains, ... | 2017 | 28731389 |