| [diagnostic value of urine hbv dna for hepatitis b virus-associated glomerulonephritis]. | to investigate the potential value of urine hepatitis b virus (hbv) dna as a new noninvasive diagnostic indicator for hbv-associated glomerulonephritis (hbv-gn). | 2014 | 25413080 |
| coding algorithms for identifying patients with cirrhosis and hepatitis b or c virus using administrative data. | despite the use of administrative data to perform epidemiological and cost-effectiveness research on patients with hepatitis b or c virus (hbv, hcv), there are no data outside of the veterans health administration validating whether international classification of disease, ninth revision, clinical modification (icd-9-cm) codes can accurately identify cirrhotic patients with hbv or hcv. the validation of such algorithms is necessary for future epidemiological studies. | 2015 | 25335773 |
| the validation of the 2010 american association for the study of liver diseases guideline for the diagnosis of hepatocellular carcinoma in an endemic area. | hepatocellular carcinoma (hcc) diagnosis could be made with one typical imaging study in a cirrhotic liver by the guideline of the american association for the study of liver diseases (aasld) in 2010. patients with hepatitis b who may not have fully developed cirrhosis could be applied. we aim to retrospectively analyze and validate the diagnostic power of the 2010 guideline in an hcc endemic area (taiwan). | 2015 | 25092265 |
| prediction of spontaneous hbeag seroconversion in hbeag-positive chronic hepatitis b patients during the immune clearance phase. | spontaneous hepatitis b virus (hbv) e antigen (hbeag) seroconversion is associated with reduced risk of liver-related complications, but is poorly understood. in this study, 113 chronic hepatitis b patients in the immune active hbeag-positive phase were followed up for 76 weeks. based on the outcome of liver function, hbeag, hepatitis b viral e antibody (anti-hbe) and hbv dna at week 76, 18 patients were classified as spontaneous hbeag seroconversion group (group a) and 95 patients were classifi ... | 2014 | 25088043 |
| diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis b virus detection in the routine clinical diagnostic laboratory. | hepatitis b virus (hbv) is the most common significant chronic viral infection world-wide. hepatitis b surface antigen (hbsag) has been the principal target for laboratory testing to identify active infection by hbv. we aimed to find out diagnostic validity of the liaison chemiluminescent method compared to the polymerase chain reaction (pcr) method for hbv detection in the routine clinical diagnostic laboratory. | 2014 | 24949287 |
| comparison between screening and confirmatory serological assays in blood donors in a region of south italy. | screening assays are needed in order to guarantee safety of donated blood, but a significant number of safe donations are removed from blood supply because of reactive screening results. it is important to evaluate the positive predictive value (ppv) of screening assays in order to modulate confirmatory algorithm and implement an adequate counseling. | 2014 | 24478048 |
| assessment of non-invasive models for liver fibrosis in chronic hepatitis b virus related liver disease patients in resource limited settings. | a total of 350 million individuals are affected by chronic hepatitis b virus infection world-wide. historically, liver biopsy has been instrumental in adequately assessing patients with chronic liver disease. a number of non-invasive models have been studied world-wide. | 2015 | 24152493 |
| evaluation of commercialized rapid diagnostic testing for some hepatitis b biomarkers in an area of intermediate endemicity. | hepatitis b virus (hbv) is a major public health threat. enzyme immune assay (eia) of hbsag is the screening method used in most settings, including in blood banks. other markers are used to evaluate the hbv replication, immunity and the infectious level of the patient. testing negative for hbsag, however, does not always mean the absence of infection, and testing other markers using eia is costly. this study evaluated the diagnostic reliability of commercially available non-hbsag hbv biomarkers ... | 2013 | 24004823 |
| prediction of disease reactivation in asymptomatic hepatitis b e antigen-negative chronic hepatitis b patients using baseline serum measurements of hbsag and hbv-dna. | differentiating 'inactive carriers' (ics) of hepatitis b virus (hbv) from hepatitis b e antigen-negative (hbeag[-]) patients in remission is challenging. we investigated whether serum-based monitoring of hepatitis b surface antigen (hbsag) and hbv-dna in asymptomatic hbeag(-) patients could distinguish these groups. | 2013 | 24004660 |
| response to peginterferon alfa-2a (40kd) in hbeag-negative chb: on-treatment kinetics of hbsag serum levels vary by hbv genotype. | we investigated whether hbv genotype influences on-treatment hbsag kinetics and/or the end-of-treatment hbsag levels associated with long-term virological response in hbeag-negative chronic hepatitis b patients treated with peginterferon alfa-2a±lamivudine in the phase iii trial. | 2013 | 23872601 |
| accuracy of international guidelines for identifying significant fibrosis in hepatitis b e antigen--negative patients with chronic hepatitis. | differing threshold levels of hepatitis b virus (hbv) dna and alanine aminotransferase (alt) are recommended by international guidelines for commencement of antiviral therapy. these guidelines advocate therapy for patients with significant fibrosis (metavir score ≥f2); we assessed the accuracy of these guideline-defined thresholds in identifying patients with ≥f2 fibrosis. | 2013 | 23811251 |
| performance of rapid tests for detection of hbsag and anti-hbsab in a large cohort, france. | the systematic use of rapid tests performed at points-of-care may facilitate hepatitis b virus (hbv) screening and substantially increase hbv infection awareness. the aim of this study was to evaluate the effectiveness of such tests for hbsag and anti-hbsab detection among individuals visiting a variety of healthcare centers located in a low hbv-prevalent area. | 2013 | 23183527 |
| meld score: utility and comparison with king's college criteria in non-acetaminophen acute liver failure. | to compare model for end-stage liver disease score (meld score, ms) and king's college hospital (kch) criteria for finding correlation of mortality in non-acetaminophen induced acute liver failure (nai-alf). | 2012 | 22868013 |
| hbsag quantification in clinical practice. | several standardized commercial assays for quantification of hepatitis b surface antigen (qhbsag) are now available. studies on hbsag kinetics from asia and europe have demonstrated that hbsag levels are highest during the immune-tolerant phase, become lower during immune-clearance phase and are the lowest in hepatitis b 'e' antigen (hbeag)-negative inactive low-replicative phase with a rise during hbeag-negative chronic hepatitis b (chb). combined use of hepatitis b virus-deoxyribonucleic acid ... | 2012 | 25755408 |
| serum angiotensin-converting enzyme level as a marker of fibrosis in patients with chronic hepatitis b. | and aims: hepatitis b virus (hbv) infection is a public health problem and affects nearly 350 million people worldwide. the present study was conducted in order to investigate the role of circulating angiotensin-converting enzyme (ace) in the context of renin-angiotensin-aldosterone in newly diagnosed chronic hepatitis b infection. moreover the association between liver fibrosis and serum ace levels was also investigated. | 2012 | 22277254 |
| predicting mortality across a broad spectrum of liver disease-an assessment of model for end-stage liver disease (meld), child-turcotte-pugh (ctp), and creatinine-modified ctp scores. | the role of model for end-stage liver disease (meld) among indian patients with cirrhosis is uncertain. we studied and compared meld with child-turcotte-pugh (ctp) and creatinine-modified-ctp (crctp) scores for predicting 1-, 3-, and 6-months mortality. | 2011 | 25755381 |
| clinical benefits of biochemical markers of fibrosis in egyptian children with chronic liver diseases. | the need for repetition of liver biopsy, especially in assessing the degree of fibrosis and follow-up of treatment protocols, justifies an intensive search for non-invasive alternatives. we attempted to investigate the clinical usefulness of serum fibrogenesis markers in pediatric chronic liver diseases. | 2010 | 27942306 |
| non-invasive assessment of liver fibrosis in patients with hepatitis c: shear wave elastography and colour doppler velocity profile technique versus liver biopsy. | determination of the presence and degree of liver fibrosis is essential for the prognosis and treatment of patients with chronic hepatitis c. non-invasive methods of assessing fibrosis have been developed to reduce the need for biopsy. we determined the efficacy of shear wave elastography (swe) and colour doppler velocity as non-invasive methods for the assessment of liver fibrosis compared to liver biopsy among patients with chronic hepatitis c virus (hcv) infection. | 2017 | 28262531 |
| utility of enzyme-linked immunosorbent assays to test core antigen in the diagnosis and antiviral therapy management of hepatitis c virus infections. | in this study, we evaluate the performance of the enzyme-linked immunosorbent assays (elisas) for hcv ag detection in the diagnosis and antiviral therapy management of hcv infections. for the diagnosis of an active hcv infection, the limit of detection of hcv ag corresponding to hcv rna level was approximately 7300 iu/ml; the sensitivity, specificity, positive predictive value (ppv), and negative predictive value (npv) of hcv-ag were 88.96, 100, 100, and 91.33%, respectively. the pearson's corre ... | 2016 | 27958657 |
| hepatitis c virus core antigen assay: an alternative method for hepatitis c diagnosis. | background the study aimed to evaluate a fully automated chemiluminescent immunoassay and compared it with a quantitative rna assay and anti-hcv assay to verify the utility of this automated ag assay as an alternative method for hepatitis c diagnosis. methods a total of 229 serum samples previously tested for anti-hcv concentrations by the architect anti-hcv assay, were selected for hcv rna testing by real time rt-pcr kit (shanghai zj bio-tec co., ltd) and 125 specimens were tested for hcv ag by ... | 2017 | 27614354 |
| serological tests do not predict residual fibrosis in hepatitis c cirrhotics with a sustained virological response to interferon. | liver biopsy (lb) has lost popularity to stage liver fibrosis in the era of highly effective anti-hepatitis c virus (hcv) therapy, yet diagnosis of persistent cirrhosis may have important implications following hcv eradication. as performance of serological non-invasive tests (nits) to predict residual fibrosis in non-viremic hcv patients is unknown, we investigated accuracy of nits to predict residual fibrosis in cirrhotics after a sustained virological response (svr) to interferon (ifn). | 2016 | 27304619 |
| hcv ns3ag: a reliable and clinically useful predictor of antiviral outcomes in genotype 1b hepatitis c virus-infected patients. | since hepatitis c virus (hcv) non-structural 3 (ns3) protease inhibitor (pi) combined with pegylated interferon/ribavirin (pr) has been approved for chronic hcv genotype (gt) 1b infection, a reliable and clinically useful predictor combining with serum hcv rna to predict early virologic response, breakthrough, and relapse is important during hcv antiviral treatment. we evaluated the role of hcv ns3 antigen (hcv ns3ag) on the prediction of virologic response in patients with hcv gt1b during pr or ... | 2016 | 27173787 |
| role of signal-to-cut-off ratios of anti-hepatitis c virus antibody by enzyme immunoassays along with id-nat for screening of whole blood donors in india. | the use of elevated signal-to-cut off ratios (s/co) as an alternate to further supplemental testing (i.e., riba) has been included in the guidelines provided by the centres for disease control and prevention for hcv diagnostic purposes since 2003. with availability of screening by nat and non availability of riba, further confirmation of hcv infection has been possible at the molecular level (rna). | 2017 | 27011676 |
| hepatitis c viral kinetic changes in a retrospective cohort study of chronic hepatitis c virus egyptian patients on pegylated interferon and ribavirin therapy. | the aim of this study was to determine the relative importance of the kinetics of antiviral response compared to baseline host and virological factors for predicting treatment outcome. a retrospective analysis of 285 chronic hepatitis c virus (hcv) patients, encompassing genotypes 4 treated with peginterferon alpha-2a and ribavirin, was performed. baseline characteristics were compared across hcv genotypes and pretreatment factors associated with rapid virological response (rvr) were identified. ... | 2016 | 26982165 |
| phase ii study of personalized peptide vaccination with both a hepatitis c virus-derived peptide and peptides from tumor-associated antigens for the treatment of hcv-positive advanced hepatocellular carcinoma patients. | objective. to evaluate safety and immune responses of personalized peptide vaccination (ppv) for hepatitis c virus- (hcv-) positive advanced hepatocellular carcinoma (hcc). patients and methods. patients diagnosed with hcv-positive advanced hcc were eligible for this study. a maximum of four hla-matched peptides were selected based on the preexisting igg responses specific to 32 different peptides, which consisted of a single hcv-derived peptide at core protein positions 35-44 (c-35) and 31 pept ... | 2015 | 26539554 |
| rapid prediction of treatment futility of boceprevir with peginterferon-ribavirin for taiwanese treatment experienced hepatitis c virus genotype 1-infected patients. | the efficacy and safety of the boceprevir (boc)-containing triple therapy in taiwanese treatment-experienced patients remains elusive. after 4 weeks of peginterferon/ribavirin lead-in therapy, patients with cirrhosis or previous null-response received triple therapy for 44 weeks; whereas others received 32 weeks of triple therapy followed by 12 weeks of peginterferon/ribavirin therapy. patients with hcv rna > 100 iu/ml at week 12 or with detectable hcv rna at week 24 of treatment were viewed as ... | 2015 | 26368130 |
| the clinical significance of hcv core antigen detection during telaprevir/peg-interferon/ribavirin therapy in patients with hcv 1 genotype infection. | direct-acting antiviral drugs (daa) regimen improve the svr rate. however, adverse effects often lead to therapy interruption. this underlines the importance to find some predictive parameters of response in order to consider the possibility of a shorter time of antiviral treatment in the appearance of adverse effects without affecting the success of the therapy. | 2015 | 26209382 |
| clinical characteristics and biopsy accuracy in suspected cases of sjögren's syndrome referred to labial salivary gland biopsy. | labial salivary gland biopsy (lsgb) is the most important diagnostic tool for the diagnosis of sjögren's syndrome (ss), but its diagnostic value is rarely studied. this study assessed the sensibility and specificity of lsgb, and the clinical profiles of patients who were referred for biopsy. | 2015 | 25887888 |
| utility of hepatitis c viral load monitoring on direct-acting antiviral therapy. | hepatitis c virus (hcv) rna loads serve as predictors of treatment response during interferon-based therapy. we evaluated the predictive ability of hcv rna levels at end of treatment (eot) for sustained virologic response (svr12) during interferon-sparing direct-acting antiviral therapies. | 2015 | 25733369 |
| concordance of sustained virological response 4, 12, and 24 weeks post-treatment with sofosbuvir-containing regimens for hepatitis c virus. | historically, clinical trials of regimens to treat chronic infection with hepatitis c virus (hcv) have used, as their primary efficacy endpoint, a sustained virological response (svr)—defined as hcv rna levels below a designated threshold of quantification—24 weeks after the end of treatment (svr24). more recently, regulatory authorities have begun to accept svr at 12 weeks post-treatment (svr12) as a valid efficacy endpoint because of its high rate of concordance with svr24. however, the concor ... | 2015 | 25314116 |
| association of interferon-γ-induced protein-10 serum levels with virological responses to peg-interferon-based therapy in hepatitis c virus genotype 1 or 2 chronically infected chinese patients. | interferon (ifn)-γ-induced protein-10 (ip-10) serum level has been shown be associated with viral response in chronic hepatitis c (chc) patients. however, little is known in chinese population. we determined ip-10 serum levels in chinese chc patients undergoing peg-ifn-based therapy. predictive role of ip-10 level for virological responses was accessed. | 2014 | 25263691 |
| comparison of hepatitis c virus rna and antibody detection in dried blood spots and plasma specimens. | current diagnostic tests for hepatitis c virus (hcv) involve phlebotomy and serologic testing for hcv antibodies (anti-hcv) and rna, which are not always feasible. dried blood spots (dbs) present a minimally invasive sampling method and are suitable for sample collection, storage and testing. | 2014 | 24529844 |
| body mass index and liver stiffness affect accuracy of ultrasonography in detecting steatosis in patients with chronic hepatitis c virus genotype 1 infection. | few studies have evaluated the accuracy of ultrasonography in detecting steatosis in patients with chronic hepatitis c. we assessed its accuracy in detecting steatosis and factors that affect its diagnostic performance in consecutive patients with chronic hepatitis c virus genotype 1 infection. | 2014 | 24112998 |
| a diagnostic score for the prediction of spontaneous resolution of acute hepatitis c virus infection. | il28b polymorphisms, jaundice, decline in hcv-rna, ip-10, and gender have been proposed to be indicative of spontaneous clearance of acute hepatitis c virus infection. the aim of this study was to define a score enabling the discrimination of patients with spontaneous clearance of hcv from those with development of viral persistence and need for early antiviral treatment. | 2013 | 23850880 |
| the influence of single and combined il28b polymorphisms on response to treatment of chronic hepatitis c. | three single nucleotide polymorphisms (snps) near il28b gene were shown to be highly predictive of sustained virological response (svr) in patients with chronic hepatitis c virus (hcv) infection. | 2013 | 23831131 |
| treatment optimization and prediction of hcv clearance in patients with acute hcv infection. | the lack of consensus on the optimal timing, regimen, and duration of treatment, in patients with acute hcv infection, stimulates the research on both favourable outcome predictors and individualized treatment regimens. this study aimed at investigating the impact of il28b snp rs12979860 alone or in combination with hla class ii alleles in both predicting spontaneous viral clearance and individualizing treatment strategies for patients with hcv persistence, after acute hcv exposure. | 2013 | 23587473 |
| earlier sustained virologic response end points for regulatory approval and dose selection of hepatitis c therapies. | trials of therapies for chronic hepatitis c have used detection of hepatitis c virus (hcv) at week 24 of follow-up (sustained virologic response [svr] 24) as a primary end point. however, there is increasing evidence that most patients who have an svr at earlier time points (such as svr12) maintain it until week 24. use of earlier time points for key regulatory decisions (svr12) and dose selection (svr4) could facilitate hcv drug development. | 2013 | 23470616 |
| prediction of sustained virologic response based on week 4 and week 12 response in hepatitis c virus genotype 1 patients treated with peginterferon and ribavirin: assessment in a favorable il28b allele-prevalent area. | the aim of this study was to evaluate rapid virologic response (rvr) rate after peginterferon (pegifn) and ribavirin (rbv) dual combination therapy in korean hepatitis c virus (hcv) genotype 1 patients whose il28b polymorphism is generally favorable. this study also assessed the value of rvr in predicting sustained virologic response (svr). | 2013 | 23306941 |
| [antiviral activity of polyprenylphosphates in experimental infection caused by hepatitis c virus in vitro]. | study antiviral effect of sodium polyprenylphosphate (ppp) in experimental infection model caused by hepatitis c virus (hcv) in cell culture. | 2012 | 23163044 |
| baseline factors and early viral response (week 4) to antiviral therapy with peginterferon and ribavirin for predicting sustained virologic response in patients infected with hepatitis c virus genotype 1: a multicenter study. | both baseline predictive factors and viral response at week 4 of therapy are reported to have high predictive ability for sustained virologic response to peginterferon and ribavirin combination therapy in patients with hepatitis c virus (hcv) genotype 1. however, it is not clear how these baseline variables and week 4 response should be combined to predict sustained virologic response. in this multicenter study, the authors investigated the impact of baseline predictive factors on the predictive ... | 2013 | 23154876 |
| il28b favorable genotype and ultrarapid viral response as the earliest treatment predictors of a sustained viral response in a georgian cohort infected with the hepatitis c genotype 1. | the early identification of factors contributing to the successful treatment of hepatitis c infection is important for researchers and clinicians. studies carried out on the role of an ultrarapid viral response (urvr) for the prediction of a sustained viral response (svr) have shown its high positive predictive value (ppv). however, data on the combined effect of urvr with il28b genotypes for the prediction of svr are lacking. our aim was to study the role of urvr and il28b genotypes in the pred ... | 2012 | 22569080 |
| hepatic cell-type specific gene expression better predicts hcv treatment outcome than il28b genotype. | cell-type specific expression patterns of hepatic interferon-stimulated genes (isgs) and single nucleotide polymorphisms (snps) near the il28b gene are associated with response to interferon-based therapy in patients with chronic hepatitis c virus (hcv) infection. it is not known how the il28b genotype influences the isg expression pattern and which is a better predictor of treatment response. | 2012 | 22285807 |
| non-invasive fibrosis score for hepatitis delta. | identifying advanced fibrosis in chronic hepatitis delta patients and thus in need of urgent treatment is crucial. to avoid liver biopsy, non-invasive fibrosis scores may be helpful but have not been evaluated for chronic hepatitis delta yet. | 2017 | 27428078 |
| self-reported hepatitis a vaccination as a predictor of hepatitis a virus antibody protection in u.s. adults: national health and nutrition examination survey 2007-2012. | to estimate the predictive value of self-reported hepatitis a vaccine (hepa) receipt for the presence of hepatitis a virus (hav) antibody (anti-hav) from either past infection or vaccination, as an indicator of hav protection. | 2015 | 26116252 |
| virus removal capacity at varying ionic strength during nanofiltration of alphanine® sd. | nanofiltration is incorporated into the manufacturing processes of many protein biopharmaceuticals to enhance safety by providing the capacity to retain pathogens while allowing protein drugs to pass through the filter. retention is mainly a function of size; however, the shape of the pathogen may also influence retention. the ability of the viresolve(®) pro nanofilter to remove different sized viruses during the manufacture of a coagulation factor ix (alphanine(®) sd) was studied at varying ion ... | 2014 | 24999080 |
| inactivation effect of standard and fractionated electron beam irradiation on enveloped and non-enveloped viruses in a tendon transplant model. | background: for increasing allograft tendon safety in reconstructive surgery, an effective sterilization method achieving sterility assurance including viruses without impairing the grafts properties is needed. fractionated electron beam (ebeam) has shown promising in vitro results. the proof of sufficient virus inactivation is a central part of the process validation. methods: the ebeam irradiation of the investigated viruses was performed in an optimized manner (oxygen content < 0.1%, -78 °c). ... | 2012 | 22896764 |
| use of a comprehensive polymerase chain reaction system for diagnosis of ocular infectious diseases. | to measure the genomic dna of ocular infectious pathogens in ocular fluids and to analyze the clinical relevance of these pathogens in uveitis and endophthalmitis. | 2013 | 23664179 |
| diagnosis and treatment of acute retinal necrosis: a report by the american academy of ophthalmology. | to evaluate the available evidence in peer-reviewed publications about the diagnosis and treatment of acute retinal necrosis (arn). | 2017 | 28094044 |
| development and validation of new diagnostic criteria for acute retinal necrosis. | the purposes of this study are to develop and validate new diagnostic criteria for acute retinal necrosis (arn) based on the ocular findings, clinical course, and virologic testing of intraocular fluids. | 2015 | 25492579 |
| varicella-zoster virus immunity among health care workers in catalonia. | to determine varicella-zoster virus (vzv) immunity among healthcare workers (hcws). cross-sectional study. | 2014 | 25219564 |
| validity of medical record documented varicella-zoster virus among unvaccinated cohorts. | a varicella diagnosis or verification of disease history by any healthcare provider is currently accepted for determining evidence of immunity by the advisory committee on immunization practices (acip). | 2013 | 23807363 |
| neurosurgery and human immunodeficiency virus in the era of combination antiretroviral therapy: a review. | human immunodeficiency virus (hiv) is a global health problem. it renders the central nervous system susceptible to infectious and noninfectious diseases. hiv-positive individuals may present to neurosurgical services with brain lesions of unknown etiology. the differential diagnosis in these cases is broad, including opportunistic infections and malignancies, and investigation should be tailored accordingly. opportunistic infections of the central nervous system can be complicated by hydrocepha ... | 2017 | 27081898 |
| can epstein-barr virus dna load in nasopharyngeal brushings or whole blood predict recurrent nasopharyngeal carcinoma in a non-endemic region? a prospective nationwide study of the dutch head and neck oncology cooperative group. | this study estimated the value of quantitative measurements of ebv markers in the clinical management of nasopharyngeal carcinoma in a non-endemic area. the aim was to predict prognosis and detect recurrent and residual disease. in 72 patients, ebv dna load in blood and nasopharyngeal brushes, and iga vca-p18 and ebna1 in plasma were measured at different time points. at diagnosis and post-treatment, a cut-off value was used for detecting disease [positive (ppv) and negative (npv) predictive val ... | 2016 | 25929413 |
| trans-oral brush biopsies and quantitative pcr for ebv dna detection and screening of nasopharyngeal carcinoma. | to evaluate a newly developed noninvasive ambulatory, quantitative polymerase chain reaction (q-pcr) epstein-barr virus (ebv) dna detection and screening system (np screen™) for nasopharyngeal carcinoma (npc). | 2014 | 24486777 |
| performance of point-of-care urine test in diagnosing tuberculosis suspects with and without hiv infection in selected peripheral health settings of addis ababa, ethiopia. | there are few rapid point-of-care tests (poct) for tuberculosis (tb) for use in resource-constrained settings with high levels of human immunodeficiency virus (hiv). this hinders early tuberculosis (tb) treatment. this cross-sectional study evaluates the recently developed urine determine tuberculosis lipoarabinomannan (tb lam) antigen test. a total of 122 participants with signs and symptoms of tb, including 21 (17.1%) participants positive for hiv, were enrolled from september 2011 to march 20 ... | 2017 | 28137314 |
| usefulness of bronchoscopic probe-based confocal laser endomicroscopy in the diagnosis of pneumocystis jirovecii pneumonia. | probe-based confocal laser endomicroscopy (pcle) is a novel technique that provides in vivo microscopic imaging of the distal lung. we hypothesized that the intra-alveolar exudates characterizing pneumocystis jirovecii pneumonia (pjp) can be identified by pcle in vivo and help in its diagnosis. | 2016 | 27362271 |
| quantitative and functional antibody responses to the 13-valent conjugate and/or 23-valent purified polysaccharide vaccine in aging hiv-infected adults. | the number of aging human immunodeficiency virus-infected (hiv+) individuals living in the united states has substantially grown over the past two decades. advanced age and hiv infection both increase susceptibility to streptococcus pneumoniae infection due to b cell dysfunction. the combined impact of these factors on pneumococcal vaccine responses remains unknown. | 2016 | 27158552 |
| provocation proven drug allergy in thai children with adverse drug reactions. | adverse drug reactions (adrs) are a common healthcare problem. the drug provocation test (dpt) is a gold standard for adr diagnosis. | 2016 | 26994627 |
| alterations in serotype-specific b cell responses to the 13-valent pneumococcal conjugate vaccine in aging hiv-infected adults. | advanced age and human immunodeficiency virus (hiv) infection are associated with increased pneumococcal disease risk. the impact of these factors on cellular responses to vaccination is unknown. | 2016 | 26707220 |
| parallel enzyme-linked immunosorbent assay screening for human immunodeficiency virus among blood donors in five chinese blood centres: a retrospective analysis. | to evaluate the strategy of parallel screening with different enzyme-linked immunosorbent assays (elisa) among chinese blood donors. | 2015 | 26104756 |
| use of real time polymerase chain reaction for detection of m. tuberculosis, m. avium and m. kansasii from clinical specimens. | the incidence of m. tuberculosis (mtb) and non tuberculous mycobacterium species (ntms) like m. avium and m. kansasii has increased due to human immunodeficiency virus (hiv) epidemic. therefore accurate, rapid and cost effective methods for the identification of these ntms and mtb are greatly needed for appropriate tb management. thus in this study we evaluated the performance of lightcycler(®) mycobacterium detection assay to detect mtb, m. avium and m. kansasii in sputum specimens. | 2015 | 25884439 |
| serum and bal beta-d-glucan for the diagnosis of pneumocystis pneumonia in hiv positive patients. | the diagnosis of patients with pulmonary infiltrates and human immunodeficiency virus (hiv) infection remains a challenge. in current clinical practice the gold standard for pneumocystis jirovecii pneumonia (pcp) diagnosis remains the identification of the organism in bronco alveolar lavage (bal) using microscopy (e.g., silver stain). (1->3)-β -d-glucan (bg) is a polysaccharide that is present within the cell wall of pneumocystis and other fungi. | 2014 | 25448310 |
| association between oral candidiasis and low cd4+ count among hiv positive patients in hoima regional referral hospital. | the aim of this study was to determine the prevalence of human immune virus (hiv) related oral lesions and their association with cluster of differentiation 4 (cd4+) count among treatment naïve hiv positive patients. | 2014 | 25432363 |
| first prospective comparison of genotypic vs phenotypic tropism assays in predicting virologic responses to maraviroc (mvc) in a phase 3 study: modern. | modern (a4001095) was the first prospective phase 3 study comparing genotype vs phenotype (trofile™) tropism assessments. | 2014 | 25394028 |
| clinical application evaluation of two fourth-generation human immunodeficiency virus (hiv) screening assays in west china hospital. | fourth-generation human immunodeficiency virus (hiv) screening assays have been used in many laboratories. the elecsys® hiv combi pt assay is a new kind of fourth-generation hiv screening assay developed to allow earlier detection of seroconversion. | 2015 | 24797498 |
| a comparison of two visual inspection methods for cervical cancer screening among hiv-infected women in kenya. | to determine the optimal strategy for cervical cancer screening in women with human immunodeficiency virus (hiv) infection by comparing two strategies: visual inspection of the cervix with acetic acid (via) and via followed immediately by visual inspection with lugol's iodine (via/vili) in women with a positive via result. | 2014 | 24700979 |
| utility of total lymphocyte count as a surrogate for absolute cd4 count in the adult indian hiv population: a prospective study. | standard methods of cd4 counts and plasma viral load estimation require specialized equipment, highly trained personnel and are extremely expensive. this remains a major challenge for the initiation of anti-retroviral therapy for patients in resource-limited settings. | 2014 | 24678463 |
| comparative evaluation of the bio-rad geenius hiv-1/2 confirmatory assay and the bio-rad multispot hiv-1/2 rapid test as an alternative differentiation assay for clsi m53 algorithm-i. | the clsi-m53-a, criteria for laboratory testing and diagnosis of human immunodeficiency virus (hiv) infection; approved guideline includes an algorithm in which samples that are reactive on a 4th generation eia screen proceed to a supplemental assay that is able to confirm and differentiate between antibodies to hiv-1 and hiv-2. the recently ce-marked bio-rad geenius hiv-1/2 confirmatory assay was evaluated as an alternative to the fda-approved bio-rad multispot hiv-1/hiv-2 rapid test which has ... | 2013 | 24342484 |
| the role of genetic polymorphisms in cytochrome p450 and effects of tuberculosis co-treatment on the predictive value of cyp2b6 snps and on efavirenz plasma levels in adult hiv patients. | efavirenz (efv) exhibits interindividual pharmacokinetic variability caused by differences in cytochrome p450 (cyp) expression. most tuberculosis (tb) drugs interact with the cyp metabolizing enzymes, while the clinical validity of genotyping in predicting efv plasma levels in rwandan subjects is not known. we investigated in patients co-infected with human immunodeficiency virus (hiv) and tb recruited in rwanda the effects of 10 snps in five drug-metabolizing enzymes on efv plasma levels and tr ... | 2014 | 24316028 |
| predictive value of interferon-gamma release assays for postpartum active tuberculosis in hiv-1-infected women. | data on the prognostic utility of interferon-gamma release assays (igras) for active tuberculosis (tb) among human immunodeficiency virus 1 (hiv-1) infected individuals are limited. | 2013 | 24200267 |
| cell surface markers for t and b lymphocytes activation and adhesion as putative prognostic biomarkers for head and neck squamous cell carcinoma. | the study population comprised hnscc patients, risk-positive controls (tabagism and alcoholism habits), and risk-negative controls (without risk factors). significant increases in the activation status of cd4(+)and cd8(+) t-cells, and higher migration potentials of lymphocytes were observed in hnscc patients compared with control groups. although decreased frequency of cd19(+)-b lymphocytes was observed in hsncc patients, a higher percentage of hla-dr(+)cd19(+)-b lymphocytes was detected in thes ... | 2013 | 23994583 |
| the health-system benefits and cost-effectiveness of using mycobacterium tuberculosis direct nucleic acid amplification testing to diagnose tuberculosis disease in the united states. | the utility of mycobacterium tuberculosis direct nucleic acid amplification testing (mtd) for pulmonary tuberculosis disease diagnosis in the united states has not been well described. | 2013 | 23697743 |
| blood screening for human immunodeficiency virus: a new algorithm to reduce the false-positive results. | the objectives to this study were to evaluate the performance of an anti-human immunodeficiency virus (hiv) blood screening test and propose a new screening algorithm for blood banks routinely using nucleic acid amplification testing (nat) to reduce false-positive results. | 2013 | 23635038 |
| detection of antenatal depression in rural hiv-affected populations with short and ultrashort versions of the edinburgh postnatal depression scale (epds). | risk of antenatal depression has been shown to be elevated in southern africa and can impact maternal and child outcomes, especially in the context of the human immunodeficiency virus (hiv). brief screening methods may optimize access to care during pregnancy, particularly where resources are scarce. this research evaluated shorter versions of the edinburgh postnatal depression scale (epds) to detect antenatal depression. this cross-sectional study at a large primary health care (phc) facility r ... | 2013 | 23615932 |
| comparative performance of electrochemiluminescence immunoassay and eia for hiv screening in a multiethnic region of china. | the recent approval of 4th generation hiv tests has forced many laboratories to decide whether to shift from 3rd to these tests. there are limited published studies on the comparative evaluation of these two different assays. we compare the performance of fourth-generation electrochemiluminescence immunoassay (chia) and third-generation enzyme linked immunosorbent assay (eia) for human immunodeficiency virus (hiv) screening and gauge whether the shift from eia to chia could be better in a multie ... | 2012 | 23144740 |
| implementing early infant diagnosis of hiv infection at the primary care level: experiences and challenges in malawi. | malawi's national guidelines recommend that infants exposed to the human immunodeficiency virus (hiv) be tested at 6 weeks of age. rollout of services for early infant diagnosis has been limited and has resulted in the initiation of antiretroviral therapy (art) in very few infants. | 2012 | 22984315 |
| ziehl-neelsen microscopy in the diagnosis of tuberculosis in settings of high human immunodeficiency virus prevalence. | to determine the accuracy of ziehl-neelsen microscopy in the diagnosis of tb in setings of high hiv prevalence. | 2012 | 26852457 |
| predictors of extracapsular extension in hpv-associated oropharyngeal cancer treated surgically. | extracapsular extension (ece) in cervical metastatic lymph nodes remains an indication for adding chemotherapy for patients with oropharyngeal squamous cell carcinoma (opscc). the aim of this study is to identify specific imaging characteristics on computed tomography (ct) scan that are predictive of ece in order to better risk stratify patients preoperatively. | 2017 | 28109475 |
| post-treatment 18f-fdg-pet/ct versus contrast-enhanced ct in patients with oropharyngeal squamous cell carcinoma: comparative effectiveness study. | to compare the accuracy of same-day therapy-assessment pet/computed tomography (pet/ct) and conventional contrast-enhanced computed tomography (cect) in patients with oropharyngeal squamous cell carcinoma (opscc). | 2017 | 28099264 |
| molecular triage of premalignant lesions in liquid-based cervical cytology and circulating cell-free dna from urine, using a panel of methylated human papilloma virus and host genes. | clinically useful molecular tools to triage women for a biopsy upon referral to colposcopy are not available. we aimed to develop a molecular panel to detect cervical intraepithelial neoplasia (cin) grade 2 or higher lesions (cin2(+)) in women with abnormal cervical cytology and high-risk hpv (hpv(+)). we tested a biomarker panel in cervical epithelium dna obtained from 211 women evaluated in a cervical cancer clinic in chile from 2006 to 2008. results were verified in a prospective cohort of 10 ... | 2016 | 27671338 |
| a pilot study to compare the detection of hpv-16 biomarkers in salivary oral rinses with tumour p16(ink4a) expression in head and neck squamous cell carcinoma patients. | human papilloma virus-16 (hpv-16) infection is a major risk factor for a subset of head and neck squamous cell carcinoma (hnscc), in particular oropharyngeal squamous cell carcinoma (opscc). current techniques for assessing the hpv-16 status in hnscc include the detection of hpv-16 dna and p16(ink4a) expression in tumor tissues. when tumors originate from hidden anatomical sites, this method can be challenging. a non-invasive and cost-effective alternative to biopsy is therefore desirable for hp ... | 2016 | 26940728 |
| improving the performance of reflex human papilloma virus (hpv) testing in triaging women with atypical squamous cells of undetermined significance (ascus): a restrospective study in a tertiary hospital in united arab emirates (uae). | cervical cancer is the second commonest cancer in women worldwide. infection with oncogenic types of human papillomavirus (hpv) is the most important risk factor for developing cervical cancer. reflex high risk hpv (hr-hpv) testing is of significant value in the assessment of papanicolaou (pap) smear results where ascus are identified. | 2016 | 26747717 |
| adult vaccination disparities among foreign-born populations in the u.s., 2012. | foreign-born persons are considered at higher risk of undervaccination and exposure to many vaccine-preventable diseases. information on vaccination coverage among foreign-born populations is limited. | 2014 | 25300733 |
| identification of oropharyngeal squamous cell carcinomas with active hpv16 involvement by immunohistochemical analysis of the retinoblastoma protein pathway. | viral oncogene rna expression is regarded as reliable biomarker to identify oropharyngeal squamous cell carcinomas (opscc) with active hpv16 involvement. this study addressed whether the expression profile of the cellular proteins p16(ink4a), prb, cyclin d1 and p53 provide surrogate marker combinations that identify opscc with active hpv16 in situations where only formalin-fixed biopsies are available. protein expression was analyzed by immunohistochemistry on tissue microarrays created from 188 ... | 2013 | 23457055 |
| identification of past and recent parvovirus b19 infection in immunocompetent individuals by quantitative pcr and enzyme immunoassays: a dual-laboratory study. | parvovirus b19 (b19v) is a member of the family parvoviridae, genus erythrovirus. b19v-specific igg and igm react differently against conformational and linear epitopes of vp1 and vp2 antigens, leading to the development of igg avidity and epitope type specificity (ets) enzyme immunoassays (eias) for distinguishing past from recent infection. additionally, b19v viral load determination (by quantitative pcr [qpcr]) is increasingly used in the staging of b19v infection. in this study, the utility ... | 2014 | 24403307 |
| antiviral effect of lithium chloride on infection of cells by porcine parvovirus. | porcine parvovirus (ppv) causes reproductive failure in pigs, which leads to economic losses to the industry. as reported previously, licl efficiently impairs the replication of a variety of viruses, including the coronavirus infectious bronchitis virus (ibv), transmissible gastroenteritis virus (tgev), and pseudorabies herpesvirus. we demonstrate for the first time that inhibition of ppv replication in swine testis (st) cells by licl is dose-dependent, and that the antiviral effect of licl occu ... | 2015 | 25663217 |
| the vp3 factor from viruses of birnaviridae family suppresses rna silencing by binding both long and small rna duplexes. | rna silencing is directly involved in antiviral defense in a wide variety of eukaryotic organisms, including plants, fungi, invertebrates, and presumably vertebrate animals. the study of rna silencing-mediated antiviral defences in vertebrates is hampered by the overlap with other antiviral mechanisms; thus, heterologous systems are often used to study the interplay between rna silencing and vertebrate-infecting viruses. in this report we show that the vp3 protein of the avian birnavirus infecti ... | 2012 | 23049903 |
| evaluation of the point-of-care becton dickinson veritor™ rapid influenza diagnostic test in kenya, 2013-2014. | we evaluated the performance of the becton dickinson veritor™ system flu a + b rapid influenza diagnostic test (ridt) to detect influenza viruses in respiratory specimens from patients enrolled at five surveillance sites in kenya, a tropical country where influenza seasonality is variable. | 2017 | 28077093 |
| comparison of directigen flu a+b with real time pcr in the diagnosis of influenza. | early diagnosis and treatment of patients with influenza is the reason why physicians need rapid high-sensitivity influenza diagnostic tests that require no complex lab equipment and can be performed and interpreted within 15 min. the aim of this study was to compare the rapid directigen flu a+b test with real time pcr for detection of influenza viruses in the republic of macedonia. | 2016 | 26933779 |
| transient expression of the influenza a virus pb1-f2 protein using a plum pox virus-based vector in nicotiana benthamiana. | pb1-f2 protein of influenza a virus (iav) was cloned in a plum pox virus (ppv) genome-based vector and attempts to express it in biolistically transfected nicotiana benthamiana plants were performed. the vector-insert construct replicated in infected plants properly and was stable during repeated passage by mechanical inoculation, as demonstrated by disease symptoms and immunoblot detection of ppv capsid protein, while pb1-f2-specific band was more faint. we showed that it was due its low solubi ... | 2014 | 25283863 |
| heterologous rna-silencing suppressors from both plant- and animal-infecting viruses support plum pox virus infection. | hcpro, the rna-silencing suppressor (rss) of viruses belonging to the genus potyvirus in the family potyviridae, is a multifunctional protein presumably involved in all essential steps of the viral infection cycle. recent studies have shown that plum pox potyvirus (ppv) hcpro can be replaced successfully by cucumber vein yellowing ipomovirus p1b, a sequence-unrelated rss from a virus of the same family. in order to gain insight into the requirement of a particular rss to establish a successful p ... | 2012 | 22513385 |
| infectious risk factors for individual postweaning multisystemic wasting syndrome (pmws) development in pigs from affected farms in spain and denmark. | two prospective longitudinal studies in 13 postweaning multisystemic wasting syndrome (pmws)-affected farms from spain (n=3) and denmark (n=10) were performed. blood samples from pigs were longitudinally collected from 1st week until the occurrence of the pmws outbreak. wasted and healthy age-matched pigs were euthanized, necropsied and histopathologically characterised. pmws diagnosis was confirmed by means of lymphoid lesions and detection of porcine circovirus type 2 (pcv2) in these tissues b ... | 2012 | 22884005 |
| reduction of spiked porcine circovirus during the manufacture of a vero cell-derived vaccine. | porcine circovirus-1 (pcv1) was recently identified as a contaminant in live rotavirus vaccines, which was likely caused by contaminated porcine trypsin. the event triggered the development of new regulatory guidance on the use of porcine trypsin which shall ensure that cell lines and porcine trypsin in use are free from pcv1. in addition, manufacturing processes of biologicals other than live vaccines include virus clearance steps that may prevent and mitigate any potential virus contamination ... | 2014 | 24560672 |
| mechanistic evaluation of virus clearance by depth filtration. | virus clearance by depth filtration has not been well-understood mechanistically due to lack of quantitative data on filter charge characteristics and absence of systematic studies. it is generally believed that both electrostatic interactions and sized based mechanical entrapment contribute to virus clearance by depth filtration. in order to establish whether the effectiveness of virus clearance correlates with the charge characteristics of a given depth filter, a counter-ion displacement techn ... | 2015 | 25683459 |
| limits in virus filtration capability? impact of virus quality and spike level on virus removal with xenotropic murine leukemia virus. | virus filtration (vf) is a key step in an overall viral clearance process since it has been demonstrated to effectively clear a wide range of mammalian viruses with a log reduction value (lrv) > 4. the potential to achieve higher lrv from virus retentive filters has historically been examined using bacteriophage surrogates, which commonly demonstrated a potential of > 9 lrv when using high titer spikes (e.g. 10(10) pfu/ml). however, as the filter loading increases, one typically experiences sign ... | 2015 | 25395156 |
| a novel approach to achieving modular retrovirus clearance for a parvovirus filter. | viral filtration is routinely incorporated into the downstream purification processes for the production of biologics produced in mammalian cell cultures (mcc) to remove potential viral contaminants. in recent years, the use of retentive filters designed for retaining parvovirus (~20 nm) has become an industry standard in a conscious effort to further improve product safety. since retentive filters remove viruses primarily by the size exclusion mechanism, it is expected that filters designed for ... | 2014 | 24123923 |
| phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on b2l gene sequence. | camel contagious ecthyma (cce) is an important viral disease of camelids caused by a poxvirus of the genus parapoxvirus (ppv) of the family poxviridae. the disease has been reported in west and east of the sudan causing economical losses. however, the ppvs that cause the disease in camels of the sudan have not yet subjected to genetic characterization. at present, the ppv that cause cce cannot be properly classified because only few isolates that have been genetically analyzed. | 2015 | 26260127 |
| the potential for pneumococcal vaccination in hajj pilgrims: expert opinion. | hajj is the annual pilgrimage to mecca in the kingdom of saudi arabia, and is one of the largest mass gathering events in the world. acute respiratory tract infections are very common during hajj, primarily as a result of close contact among pilgrims, intense congestion, shared accommodation and air pollution. a number of vaccines are (or have been) recommended for hajj pilgrims in recent years. several additional vaccines could significantly reduce the morbidity and mortality at hajj and should ... | 2013 | 23810307 |
| a pcv2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against pcv2b or combined pcv2a/2b viremia in pigs with concurrent pcv2, prrsv and ppv infection. | the predominant genotype of porcine circovirus (pcv) in the pig population today is pcv2b yet pcv2a-based commercial vaccines are considered effective in protecting against porcine circovirus associated disease. the objective of this study was to compare the ability of pcv2a- and pcv2b-based vaccines to control pcv2b viremia in a challenge model that mimics the u.s. field situation. sixty-three pigs were randomly assigned to one of eight groups. sixteen pigs were vaccinated with an experimental ... | 2013 | 23174198 |
| peste des petits ruminants virus, mauritania. | | 2014 | 24447781 |