| antivenomic approach of different crotalus durissus collilineatus venoms. | our group has previously performed a proteomic study verifying that individual variations can occur among crotalus durissus collilineatus venoms. these variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. in this way, this study aimed to evaluate the brazilian anticrotalic serum capacity in recognizing twenty-two crotalus durissus collilineatus venoms, as well as their fractions. | 2018 | 30534148 |
| the endogenous opioid system modulates defensive behavior evoked by crotalus durissus terrificus: panicolytic-like effect of intracollicular non-selective opioid receptors blockade. | there is a controversy regarding the key role played by opioid peptide neurotransmission in the modulation of panic-attack-related responses. | 2019 | 30407114 |
| crotoxin from crotalus durissus terrificus venom: in vitro cytotoxic activity of a heterodimeric phospholipase a2 on human cancer-derived cell lines. | crotoxin (ctx), a heterodimeric phospholipase present in venom of snakes of the genus crotalus, has demonstrated a broad spectrum of pharmacological properties, such as antimicrobial, hemostatic, and antitumoral. however, the precise mechanism of its cytotoxicity and antitumoral properties remains to be determined. therefore, in the present study, we isolated crotoxin (f1 ctx) through two steps deae-sepharose and heparin-sepharose ff chromatography. the c-terminal sequence of the a- and b-chain ... | 2018 | 30395843 |
| cell migration inhibition activity of a non-rgd disintegrin from crotalus durissus collilineatus venom. | in recent decades, snake venom disintegrins have received special attention due to their potential use in anticancer therapy. disintegrins are small and cysteine-rich proteins present in snake venoms and can interact with specific integrins to inhibit their activities in cell-cell and cell-ecm interactions. these molecules, known to inhibit platelet aggregation, are also capable of interacting with certain cancer-related integrins, and may interfere in important processes involved in carcinogene ... | 2018 | 30377432 |
| a biotechnological approach to immunotherapy: antivenom against crotalus durissus cascavella snake venom produced from biodegradable nanoparticles. | snakebite envenoming is a tropical disease neglected worldwide. in brazil, the crotalus durissus cascavella (cdc) snake belongs to a genus with venom of highest lethality. a search for new immunoadjuvants aimed to expand the therapeutic alternatives to improve vaccines and antivenom. this approach proposed to produce small and narrow-sized cationic cdc venom-loaded chitosan nanoparticles (chnp) able to induce antibody response against the cdc venom. the ionic gelation method induced the formatio ... | 2018 | 30287370 |
| in-depth venome of the brazilian rattlesnake crotalus durissus terrificus: an integrative approach combining its venom gland transcriptome and venom proteome. | snake venoms are complex mixtures mainly composed of proteins and small peptides. crotoxin is one of the most studied components from crotalus venoms, but many other components are less known due to their low abundance. the venome of crotalus durissus terrificus, the most lethal brazilian snake, was investigated by combining its venom gland transcriptome and proteome to create a holistic database of venom compounds unraveling novel toxins. we constructed a cdna library from c. d. terrificus veno ... | 2018 | 30270628 |
| cdna cloning, heterologous expression, protein folding and immunogenic properties of a phospholipase a2 from bothrops ammodytoides venom. | a mrna transcript that codes for a phospholipase (pla2) was isolated from a single venom gland of the bothrops ammodytoides viper. the pla2 transcript was cloned onto a pcr®2.1-topo vector and subsequently expressed heterologously in the e. coli strain m15, using the pqe30 vector. the recombinant phospholipase was named rbampla2_1, and is composed of an n-terminal fusion protein of 16 residues, along with 122 residues from the mature protein that includes 14 cysteines that form 7 disulfide bonds ... | 2019 | 30205154 |
| crotalus durissus sp. rattlesnake venom induces toxic injury in mouse sperm. | accidents involving snakebites constitute a serious public health problem in many regions around the world. however, there are no study about a possible action of snake venom on the reproductive system. herein we show that crotalus durissus sp. (linnaeus) rattlesnake venom (25 μg/kg of body weight) affected chromatin condensation, and increased the number of sperm with abnormal morphology and the sperm count. in conclusion, besides the known hazards of the c. durissus sp. venom to animal health, ... | 2018 | 30149042 |
| edema induced by a crotalus durissus terrificus venom serine protease (cdtsp 2) involves the par pathway and pkc and plc activation. | snake venom serine proteases (svsps) represent an essential group of enzymatic toxins involved in several pathophysiological effects on blood homeostasis. some findings suggest the involvement of this class of enzymatic toxins in inflammation. in this paper, we purified and isolated a new gyroxin isoform from the crotalus durissus terrificus (cdt) venom, designated as cdtsp 2, which showed significant proinflammatory effects in a murine model. in addition, we performed several studies to elucida ... | 2018 | 30111691 |
| crotoxin isolated from crotalus durissus terrificus venom modulates the functional activity of dendritic cells via formyl peptide receptors. | the crotalus durissus terrificus rattlesnake venom, its main toxin, crotoxin (ctx), and its crotapotin (ca) and phospholipase a2 (cb) subunits modulate the immune system. formyl peptide receptors (fprs) and lipoxin a4 (lxa4) are involved in ctx's effect on macrophages and neutrophils. dendritic cells (dcs) are plasticity cells involved in the induction of adaptive immunity and tolerance maintenance. therefore, we evaluated the effect of ctx, ca or cb on the maturation of dcs derived from murine ... | 2018 | 29967803 |
| electrical field stimulation-induced contractions on pantherophis guttatus corpora cavernosa and aortae. | a tetrodotoxin (ttx)-resistant mechanism is responsible for the electrical field stimulation (efs)-induced contractions and relaxations of crotalus durissus terrificus corpora cavernosa. here it was investigated whether this mechanism also occurs in corpora cavernosa and aortae of the non-venomous snake pantherophis guttatus corpora cavernosa and aortae. corpora cavernosa and aortic rings isolated from pantherophis guttatus snake were mounted in organ bath system for isometric tension recording. ... | 2018 | 29672643 |
| camelid single-domain antibodies (vhhs) against crotoxin: a basis for developing modular building blocks for the enhancement of treatment or diagnosis of crotalic envenoming. | toxic effects triggered by crotalic envenoming are mainly related to crotoxin (ctx), composed of a phospholipase a₂ (cb) and a subunit with no toxic activity (ca). camelids produce immunoglobulins g devoid of light chains, in which the antigen recognition domain is called vhh. given their unique characteristics, vhhs were selected using phage display against ctx from crotalus durissus terrificus. after three rounds of biopanning, four sequence profiles for cb (kf498602, kf498603, kf498604, and k ... | 2018 | 29596324 |
| mechanistic insights into functional characteristics of native crotamine. | the chemical composition of snake venoms is a complex mixture of proteins and peptides that can be pharmacologically active. crotamine, a cell-penetrating peptide, has been described to have antimicrobial properties and it exerts its effects by interacting selectively with different structures, inducing changes in the ion flow pattern and cellular responses. however, its real therapeutic potential is not yet fully known. bearing in mind that crotamine is a promising molecule in therapeutics, thi ... | 2018 | 29574214 |
| protocol to obtain targeted transcript sequence data from snake venom samples collected in the colombian field. | snake venoms are a mixture of different molecules that can be used in the design of drugs for various diseases. the study of these venoms has relied on strategies that use complete venom extracted from animals in captivity or from venom glands that require the sacrifice of the animals. colombia, a country with political and geographical conflicts has difficult access to certain regions. a strategy that can prevent the sacrifice of animals and could allow the study of samples collected in the fie ... | 2018 | 29571682 |
| characterization of the venom of c. d. cumanesis of colombia: proteomic analysis and antivenomic study. | the colombian rattlesnake crotalus durissus cumanensis is distributed in three geographic zones of the country: the atlantic coast, the upper valley of the magdalena river, and the eastern plains of the colombian orinoquía. its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. identification and analysis of c. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which ... | 2018 | 29462980 |
| global proteomic and functional analysis of crotalus durissus collilineatus individual venom variation and its impact on envenoming. | individual variations studies are important to understand the snakebite envenoming and to improve the antivenom production and its effectiveness. in this way, the objective of this study was a comparative analysis of intraspecific variation in the venom composition of 22 crotalus durissus collilineatus specimens through proteomic techniques. venoms were fractionated by rp-fplc, and analyzed by sds-page and mass spectrometry. although similar, chromatographic and electrophoretic profiles showed s ... | 2019 | 29462664 |
| successful reconstruction of whole mitochondrial genomes from ancient central america and mexico. | the northern and southern peripheries of ancient mesoamerica are poorly understood. there has been speculation over whether borderland cultures such as greater nicoya and casas grandes represent mesoamerican outposts in the isthmo-colombian area and the greater southwest, respectively. poor ancient dna preservation in these regions challenged previous attempts to resolve these questions using conventional genetic techniques. we apply advanced in-solution mitogenome capture and high-throughput se ... | 2017 | 29273718 |
| antitumoral effects of γcdcpli, a pla2 inhibitor from crotalus durissus collilineatus via pi3k/akt pathway on mda-mb-231 breast cancer cell. | phospholipases a2 (pla2s) overexpression is closely associated with the malignant potential of breast cancers. here, we showed for the first the antitumoral effects of γcdcpli, a pla2 inhibitor from crotalus durissus collilineatus via pi3k/akt pathway on mda-mb-231 cell. firstly, γcdcpli was more cytotoxic to mda-mb-231 breast cancer cells than other cell lines (mcf-7, hela, pc3 and a549) and did not affect the viability of non-tumorigenic breast cell (mcf 10a). in addition, γcdcpli induced modu ... | 2017 | 28765552 |
| pulmonary mechanic and lung histology induced by crotalus durissus cascavella snake venom. | this study have analyzed the pulmonary function in an experimental model of acute lung injury, induced by the crotalus durissus cascavella venom (c. d. cascavella) (3.0 μg/kg - i.p), in pulmonary mechanic and histology at 1 h, 3 h, 6 h, 12 h and 24 h after inoculation. the c. d. cascavella venom led to an increase in newtonian resistance (rn), tissue resistance (g) and tissue elastance (h) in all groups when compared to the control, particularly at 12 h and 24 h. the histeresivity (η) increased ... | 2017 | 28760509 |
| no production and potassium channels activation induced by crotalus durissus cascavella underlie mesenteric artery relaxation. | animal toxins are natural resources for pharmacological studies. the venom of crotalus durissus cascavella (c.d. cascavella) may be a source in the bio-prospecting of new anti-hypertensive agents. the aim of this study was to investigate vascular effects of the venom of c.d. cascavella in normotensive rats. studies were performed using isolated mesenteric artery segments and aortic endothelial cells. the cumulative administration of the venom of c.d. cascavella (0.001-30 μg/ml) on phenylephrine ... | 2017 | 28428070 |