| taxonomic note: a proposal for reviewing the interpretation of the camp reaction between listeria monocytogenes and rhodococcus equi. | the discrepancies between the current description of the camp test between listeria monocytogenes and rhodococcus equi in the latest edition of bergey's manual of determinative bacteriology (l. monocytogenes is described as camp test negative with r. equi) and routine findings (positive reactions are usually described in many laboratories) make it advisable to review the current interpretation of the camp test to avoid confusion among people working in microbiological laboratories. overall, 98.4 ... | 1996 | 8782698 |
| the microbiological fate of polycyclic aromatic hydrocarbons: carbon and oxygen balances for bacterial degradation of model compounds. | a series of pure bacterial strains belonging mainly to the rhodococcus and pseudomonas genera were grown on one of the following polycyclic aromatic hydrocarbons (pah) supplied as sole carbon and energy source; naphthalene, fluorene, phenanthrene, anthracene, fluoranthene and pyrene. in each case, a quantitative evaluation of the carbon repartition of the pah degraded into co2, biomass and water-soluble metabolites was carried out. in addition, the kinetics of oxygen consumption and of water-sol ... | 1996 | 8785039 |
| role of cd4+, cd8+ and double negative t-cells in the protection of scid/beige mice against respiratory challenge with rhodococcus equi. | to evaluate the contributions of t-lymphocyte subsets in pulmonary immunity against rhodococcus equi, c.b-17 scid/beige mice were adoptively transferred with splenic lymphocytes from congenic balb/c mice previously infected with r. equi. spleen cells were enriched for either cd4+ or cd8+ populations before inoculation, flow cytometry showed that each enriched population contained less than 0.5% cross contamination. groups of adoptively transferred scid/beige mice were sacrificed 6 and 13 d after ... | 1996 | 8809381 |
| 2-d crystallization of the rhodococcus 20s proteasome | the 2d crystallization method using a liquid-liquid interface has been applied to the rhodococcus 20s proteasome. two types of ordered arrays were obtained, both large enough for high-resolution analysis. the first one is a hexagonal close-packed array, whereas the second one has fourfold symmetry. by image analysis based on a real space correlation averaging technique, the close-packed array was found to be hexagonally packed but the molecules had complete rotational freedom. the fourfold array ... | 1996 | 8813003 |
| identification of intermediately virulent rhodococcus equi isolates from pigs. | we recently reported the existence of rhodococcus equi isolates with at least three virulence levels, isolated from aids patients: virulent r. equi having 15- to 17-kda antigens that kills mice with 10(6) cells, intermediately virulent r. equi having a 20-kda antigen that kills mice with 10(7) cells, and avirulent r. equi that does not kill mice with 10(8) cells or more (s. takai, y. imai, n. fukunaga, y. uchida, k. kamisawa, y. sasaki, s. tsubaki, and t. sekizaki, j. infect. dis. 172:1306-1311, ... | 1996 | 8815079 |
| bacteremia due to gordona sputi in an immunocompromised patient. | we report a case of bacteremia due to a strain identified as gordona sputi in a patient with metastatic melanoma. the origin of infection remains unknown, but extensive cutaneous lesions due to interleukin-2 treatment may have been the portal of entry. the isolate was related to g. sputi on the basis of its biochemical and genomic properties but exhibited some differences from the type strain. | 1996 | 8818912 |
| gene overexpression, purification, and identification of a desulfurization enzyme from rhodococcus sp. strain igts8 as a sulfide/sulfoxide monooxygenase. | the oxidation of dibenzothiophene to dibenzothiophene sulfone has been linked to the enzyme encoded by the sox/dszc gene from rhodococcus sp. strain igts8 (s. a. denome, c. oldfield, l. j. nash, and k. d. young, j. bacteriol. 176:6707-6717, 1994; c. s. piddington, b. r. kovacevich, and j. rambosek, appl. environ. microbiol. 61:468-475, 1995). however, this enzyme has not been characterized, and the type of its catalytic activity remains unclassified. in this work, the sox/dszc gene was overexpre ... | 1996 | 8824615 |
| rhodococcus equi: an emerging opportunistic pathogen. | rhodococcus equi is emerging as a cause of pneumonia in immunocompromised people, especially those with aids. like mycobacteria, r. equi is phagocytosed by alveolar macrophages and replicates within them. recent work is beginning to elucidate the cell and molecular biology of this opportunistic pathogen and the host immune response to it. | 1996 | 8824792 |
| attempts to find phenotypic markers of the virulence plasmid of rhodococcus equi. | four isolates of rhodococcus equi, from pneumonic foals, and containing the 85 kb virulence plasmid, a porcine isolate containing an 80 kb plasmid, and their plasmid cured derivatives, were examined for 239 phenotypic properties in an attempt to find characters other than the virulence-associated protein (vapa) which might be encoded by the virulence plasmid in organisms grown at 37 degrees c. tests chosen included those which have previously given variable results for r. equi isolates, since su ... | 1996 | 8825990 |
| equine herpesvirus type 2 (ehv-2) as a predisposing factor for rhodococcus equi pneumonia in foals: prevention of the bifactorial disease with ehv-2 immunostimulating complexes. | equine herpesvirus type 2 (ehv-2), a member of the gammaherpesvirinae subfamily, was studied in a two-phase respiratory disease complex of young foals as a predisposing factor for the secondary bacterial invasion of lungs with rhodococcus equi (r. equi). foals were immunized against ehv-2 on a farm where r. equi pneumonia regularly occurred during the last years. the immunizations were performed by using a subunit vaccine which selectively presents envelope glycoproteins of ehv-2 in a multimeric ... | 1996 | 8828122 |
| photoreactive nitrile hydratase: the photoreaction site is located on the alpha subunit. | nitrile hydratase (nhase) from rhodococcus sp. n-771 exists in active and inactive forms. the inactive nhase is immediately activated by light irradiation and changes to the active form. to characterize the photoreactive center, the inactive nhase was denatured by 6 m urea, and two kinds of subunits (alpha and beta) were separated and purified by anion-exchange chromatography. in a manner similar to the native nhase, the isolated alpha subunit showed two absorption peaks at 280 and 370 nm, which ... | 1996 | 8830032 |
| an identification scheme for rapidly and aerobically growing gram-positive rods. | an identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ... | 1996 | 8837385 |
| isolation of virulent and intermediately virulent rhodococcus equi from soil and sand on parks and yards in japan. | rhodococcus equi is an emerging opportunistic pathogen of human immunodeficiency virus-infected patients. however, little is known about the distribution of virulent and intermediately virulent r. equi in human environment. in the present study, r. equi was isolated from 173 of 234 (73.9%) samples collected from soil and sand on 115 parks and 49 yards in japan. the numbers of r. equi from soil and sand ranged from 2.5 x 10(1) to 1.2 x 10(5) per gram of sample. none of 1,294 isolates from those s ... | 1996 | 8844605 |
| cholesterol oxidase from rhodococcus equi is likely the major factor involved in the cooperative lytic process (camp reaction) with listeria monocytogenes. | the camp reaction between listeria monocytogenes and rhodococcus equi was studied by a diffusion assay. listeria monocytogenes displayed identical cooperative haemolytic effect with supernatant cultures of r. equi or with commercial cholesterol oxidase (cox). this result, even with enzymes of different sources (commercial cox is obtained from pseudomonas spp.) suggests that this enzyme secreted by r. equi has a crucial role in the synergistic haemolytic (camp) reaction with l. monocytogenes. the ... | 1996 | 8852354 |
| transcriptional regulation of the rhodococcus rhodochrous j1 nita gene encoding a nitrilase. | the 1.4-kb downstream region from a nitrilase gene (nita) of an actinomycete rhodococcus rhodochrous j1, which is industrially in use, was found to be required for the isovaleronitrile-dependent induction of nitrilase synthesis in experiments using a rhodococcus-escherichia coli shuttle vector pk4 in a rhodococcus strain. sequence analysis of the 1.4-kb region revealed the existence of an open reading frame (nitr) of 957 bp, which would encode a protein with a molecular mass of 35,100. deletion ... | 1996 | 8855219 |
| genetic differences between nitrate-negative and nitrate-positive c. pseudotuberculosis strains using restriction fragment length polymorphisms. | corynebacterium pseudotuberculosis has been classified into two biotypes according to ability to breakdown nitrate (biberstein et al., 1971). restriction enzyme analysis (rea) has shown to reflect this differentiation, but numerous bands generated by this technique make interpretation difficult (songer et al., 1988). restriction fragment length polymorphism's (rflp's) has become an accepted genetic tool and was used in this study to determine if differences in nitrate reduction and other phenoty ... | 1996 | 8861638 |
| empyema with malakoplakic-like lesions by rhodococcus equi as a presentation of hiv infection. | rhodococcus equi (corynebacterium equi) is an aerobic actinomycetes, well described as a cause of pulmonary infection in different animals as horses, pigs and cows. this pathogen has a coccobacillar aspect and a variable acid-fast stain in tissues. rare cases of human infection by rhodococcus species were described, the majority by rhodococcus equi, especially in patients with immunodeficiency syndrome (aids) in advanced stages of the disease. usually the diagnosis of infections by rhodococcus s ... | 1995 | 8868180 |
| growth of rhodococcus s1 on anthracene. | three slow-growing bacteria were isolated from a mixed culture enriched for growth on anthracene, using creosote-contaminated soil as the inoculum. organisms were shown to use anthracene by the production of a clear zone around the colony after a mineral salts agar plate was sprayed with anthracene. all three bacteria were nonmotile, nonsporulating, gram-positive rods and stained acid-fast. physiological and biochemical tests, gc content, and cell wall lipid patterns of whole cell methanolysates ... | 1996 | 8868237 |
| genetic analysis of clavibacter toxicus, the agent of annual ryegrass toxicity. | multilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates of clavibacter toxicus, the agent of annual ryegrass toxicity. these included 37 western australian (wa) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in australia over approximately 8 years. seventeen reference strains for the related genera curtobacterium, rhodococcus and arthrobacter were examined for comparison. th ... | 1996 | 8870638 |
| different rifampicin inactivation mechanisms in nocardia and related taxa. | mycolic acid-containing bacteria inactivate rifampicin in a variety of ways such as glucosylation, ribosylation, phosphorylation and decolorization. these inactivations were found to be a species-specific phenomena in nocardia and related taxa. gordona, tsukamurella and fast-growing mycobacterium modified rifampicin by ribosylation of the 23-oh group of the antibiotic. such ribosylation was not observed in rhodococcus and corynebacterium, but phosphorylation of the 21-oh group of rifampicin was ... | 1996 | 8871521 |
| serologic responses to rhodococcus equi in individuals with and without human immunodeficiency virus infection. | thirty healthy blood donors, 15 workers from horse-breeding farms, 69 human immunodeficiency virus (hiv)-negative persons at risk for hiv infection, 125 hiv-infected subjects without rhodococcus equi infection, and nine hiv-infected patients with rhodococcus equi pneumonia were evaluated in order to detect serum antibodies to rhodococcus equi precipitate-soluble antigen by an enzyme immunoassay (eia). whereas eia values for healthy donors, horse farm workers, individuals at risk for hiv infectio ... | 1996 | 8874077 |
| in vitro activity of the trinem sanfetrinem (gv104326) against gram-positive organisms. | the in vitro activity of the trinem sanfetrinem (formerly gv104326) (gv) was compared with that of vancomycin, ampicillin, and/or nafcillin against 287 gram-positive bacteria, including methicillin-resistant staphylococcus aureus and multiresistant enterococci, by the agar and microbroth dilution methods. gv demonstrated 2 to 16 times more activity than ampicillin and nafcillin against the majority of these organisms. the mic range of gv was 16 to 64 micrograms/ml for 19 enterococcus faecium str ... | 1996 | 8878596 |
| poststenotic cavitating pneumonia due to rhodococcus equi in hiv infection. | | 1996 | 8882676 |
| expression of virulence-associated antigens of rhodococcus equi is regulated by temperature and ph. | we recently reported that there are two different virulence-associated antigens correlated with virulence levels in rhodococcus equi isolates from aids patients: virulent r. equi that kills mice with 10(6) cells expresses 15- to 17-kda antigens and intermediately virulent r. equi that kills mice with 10(7) cells expresses a 20-kda antigen. environmental parameters were evaluated for their effects on the expression of these virulence-associated antigens in virulent r. equi strains by immunoblotti ... | 1996 | 8887354 |
| the incidence and consequences of failure of passive transfer of immunity on a thoroughbred breeding farm. | circulating igg concentration was determined between 12 and 24 hours after birth for 323 foals born on a thoroughbred breeding farm over 3 consecutive years. the incidence of failure of passive transfer (fpt) of maternal immunoglobulins (foal circulating igg concentration < 8 g/l) was found to be 9.6%. foals born late in the season (october to december) were found to be at increased risk for the development of fpt. the degree of assistance required at parturition and the presence of a peripartur ... | 1996 | 8893988 |
| rhodococcal pneumonia: humoral versus cell-mediated immunity. | | 1996 | 8894528 |
| use of a virulence-associated protein based enzyme-linked immunosorbent assay for rhodococcus equi serology in horses. | an enzyme-linked immunosorbent assay (elisa) was developed against rhodococcus equi using triton x-114 detergent extracted whole cell material, in which the virulence associated protein (vapa) predominated. enzymelinked immunosorbent assay titres corresponded to antibody reacting with vapa on western blots. there was considerable variation in antibody titres of nonimmunised mares and in the time when the colostrally derived antibody of their foals had declined to low or undetectable titres. in g ... | 1996 | 8894530 |
| extra-pulmonary rhodococcus equi in a thoroughbred foal. | | 1996 | 8896881 |
| allogeneic bone marrow transplantation can restore cd4+ t-lymphocyte count and immune function in idiopathic cd4+ t-lymphocytopenia. | cd4+ t-lymphocytopenia in the absence of hiv infection is a heterogeneous disorder of unknown cause. here we report a patient with idiopathic cd4+ t-lymphocytopenia, presenting with an opportunistic rhodococcus equi infection. when aplastic anemia developed subsequently, allogeneic bone marrow transplantation was performed. complete restoration of immune function was observed. we conclude that allogeneic bone marrow transplantation presents a potentially curative therapy for cd4+ t-lymphocytopen ... | 1996 | 8899203 |
| relationship between induction of macrophage chemotactic factors and formation of granulomas caused by mycoloyl glycolipids from rhodococcus ruber (nocardia rubra). | mycoloyl glycolipids cause granulomas in the lungs, liver, and spleen of mice, but the mechanism is not fully understood. to understand the role of macrophage chemotactic factors (mcfs) in granuloma formation, we prepared various mycoloyl glycolipids with different carbohydrate moieties: trehalose dimycolate (tdm), glucose mycolate (gm), mannose mycolate (mm), and fructose mycolate (fm) from rhodococcus ruber, and examined the relationship between their mcf induction in peritoneal macrophages an ... | 1996 | 8902634 |
| the beta-ketoadipate pathway and the biology of self-identity. | the beta-ketoadipate pathway is a chromosomally encoded convergent pathway for aromatic compound degradation that is widely distributed in soil bacteria and fungi. one branch converts protocatechuate, derived from phenolic compounds including p-cresol, 4-hydroxybenzoate and numerous lignin monomers, to beta-ketoadipate. the other branch converts catechol, generated from various aromatic hydrocarbons, amino aromatics, and lignin monomers, also to beta-ketoadipate. two additional steps accomplish ... | 1996 | 8905091 |
| serum antibody responses of foals to virulence-associated 15- to 17-kilodalton antigens of rhodococcus equi. | humoral immune responses in 16 foals to virulence-associated 15- to 17-kda antigens of rhodococcus equi were studied during the first fourteen weeks of life on two horse-breeding farms with a persistent incidence of r. equi infection. serum antibody levels specific for 15- to 17-kda antigens were measured by enzyme-linked immunosorbent assay and western immunoblotting. immunoglobulin g (igg) antibodies specific to 15- to 17-kda antigens were detected by all the foals. r. equi was found in the fe ... | 1996 | 8914251 |
| simultaneous utilization of pyridine and fructose by rhodococcus opacus ufz b 408 without an external nitrogen source. | a bacterium classified as rhodococcus opacus, which is able to use pyridine (a potentially growth-inhibiting substrate) as its sole source of carbon, energy and nitrogen, was isolated. in a carbon-limited chemostat culture, the kinetics was determined for growth on both pyridine and a mixture of pyridine and fructose (9 mm/22.15 mm). with growth on pyridine, stable steady states were achieved up to dilution rates of about 0.1 h-1. a further increase in the dilution rate resulted in the progressi ... | 1996 | 8920194 |
| relationship between haemagglutination and hela-cell adherence of rhodococcus equi. | in this study, 45 rhodococcus equi isolates from animals and humans were identified by the api coryne system, and serologically with monospecific antisera against capsular types 1-7, with serotypes 1 and 2 predominating. regardless of serotype, 14 out of 31 isolates from animals and one of 14 isolates from humans expressed 15-17-kd virulence-associated proteins. in hexadecane adherence studies, serotype 2 isolates generally displayed hydrophobic surfaces. in addition, isolates of serotype 1 gene ... | 1996 | 8928574 |
| failure of hyperimmune plasma to prevent pneumonia caused by rhodococcus equi in foals. | a trial was conducted on a thoroughbred stud to determine whether or not the administration of anti-rhodococcus equi hyperimmune plasma would reduce the prevalence of r equi pneumonia (rattles) in foals born in the 1992 horse breeding season. hyperimmune plasma was administered to 34 foals; another 57 foals were untreated. there was no significant difference in the number of transfused foals developing r equi pneumonia compared with the untreated foals. the time required for recovery from pneumo ... | 1995 | 8929188 |
| genetic analysis of the dsz promoter and associated regulatory regions of rhodococcus erythropolis igts8. | the dsz gene cluster of rhodococcus erythropolis igts8 comprises three genes, dsza, dszb, and dszc, whose products are involved in the conversion of dibenzothiophene (dbt) to 2-hydroxybiphenyl and sulfite. this organism can use dbt as the sole sulfur source but not as a carbon source. dsz activity is repressed by methionine, cysteine, casamino acids, and sulfate but not by dbt or dimethyl sulfoxide. we cloned 385 bp of the dna immediately 5' to dsza in front of the reporter gene lacz of escheric ... | 1996 | 8932295 |
| rhodococcus equi in capd-associated peritonitis treated with azithromycin. | | 1996 | 8941608 |
| rhodococcus equi causing bacteraemia and pneumonia in a pulmonary transplant patient. | | 1996 | 8945718 |
| a chemotaxonomic study of the lipoglycans of rhodococcus rhodnii n445 (ncimb 11279). | rhodococcus rhodnii n445 was investigated for the presence of macroamphiphilic lipoglycan. purification of a hot phenol-water extract by hydrophobic interaction chromatography allowed the resolution of three lipoglycan fractions. the two main preparations contained lipoglycans with carbohydrate compositions consistent with the presence of lipoarabinomannan and lipomannan, whilst the minor fraction appeared to contain a mixture of these two lipoglycans. the fatty acid composition of the lipoglyca ... | 1996 | 8946691 |
| [generalized rhodococcosis of fatal outcome in a patient with aids]. | | 1996 | 8949612 |
| rhodococcus equi infection in hiv-positive subjects: a retrospective analysis of 24 cases. | rhodococcus equi causes a rare infection in immunocompromised hosts. we describe 24 cases of infection in patients with aids-related complex (arc)/acquired immunodeficiency syndrome (aids). pneumonia was always the first manifestation of r. equi infection, but extrapulmonary involvement was also observed. the main sources of bacteria were sputum, bronchial washings and blood. the strains isolated were mainly susceptible to erythromycin, vancomycin, teicoplanin, rifampicin, imipenem and aminoglyc ... | 1996 | 8953675 |
| differentiation of rhodococcus species by ribotyping. | the ribosomal rna gene restriction patterns (ribotyping) was investigated with respect to the characterization of rhodococcus species and r. rhodochrous strains. chromosomal dna was prepared, digested with bamh i, blotted, and hybridized with acetylaminofluorene-labelled 16 + 23s rrna from e. coli. the type strains of seven rhodococcus species studied gave different hybridization patterns in each case. all strains tested were clearly distinguishable by ribotyping. patterns contained two to eight ... | 1996 | 8956489 |
| the use of a novel recombinant heroin esterase in the development of an illicit drugs biosensor. | | 1996 | 8958079 |
| isolation of rhodococcus rhodochrous ncimb13064 derivatives with new biodegradative abilities. | rhodococcus rhodochrous ncimb13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. mutants of ncimb13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chloroalkanes (c3-c8) is encoded by the same plasmid prtl1. however, a different genetic element(s) is required for the dehalogenation of 3-chloropropionic acid. two derivatives (p200 an ... | 1996 | 8961560 |
| routine use of pcr-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media. | a pcr-restriction fragment length polymorphism (pcr-rflp) procedure capable of rapidly identifying 28 species of clinically encountered mycobacteria was evaluated for use in the routine identification of acid-fast isolates growing in bactec 12b and 13a liquid media. pcr-rflp identified 100 of 103 acid-fast isolates recovered from 610 patient specimens submitted for culture during the study. the three isolates unidentifiable by pcr-rflp produced restriction patterns not included in the pcr-rflp a ... | 1997 | 8968884 |
| studies on the isopropylbenzene 2,3-dioxygenase and the 3-isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of rhodococcus erythropolis bd2. | the enzymes responsible for the degradation of isopropylbenzene (ipb) and co-oxidation of trichloroethene (tce) by rhodococcus erythropolis bd2 are encoded by the linear plasmid pbd2. fragments containing ipb catabolic genes were cloned from pbd2 and the nucleotide sequence was determined. by means of database searches and expression of the cloned genes in recombinant strains, we identified five clustered genes, ipba1a2a3a4c, which encode the three components of the ipb 2,3-dioxygenase system, r ... | 1996 | 8969521 |
| community-acquired bacteremia in human immunodeficiency virus-infected children in harare, zimbabwe. | hiv infection is common in mothers and their children in zimbabwe, and hiv-infected children are particularly susceptible to bacterial infections. there is little information on the etiology and outcome of hiv-related bacteremia in african children. | 1996 | 8970218 |
| radiological findings in nine aids patients with rhodococcus equi pneumonia. | rhodococcus equi (r. equi) infections have been incidentally reported as a cause of pulmonary infection in severely immunocompromised hosts, including aids patients. our purpose is to describe the radiological findings in nine aids patients with r. equi pneumonia assessed by bronchoalveolar lavage (bal), biopsies, cultures of sputum, and hemocultures. all patients were examined by chest radiographs and contrast-medium-enhanced chest ct. dense pulmonary consolidations with or without cavitations ... | 1996 | 8972317 |
| monooxygenase-like sequence of a rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic. | a dna clone from rhodococcus equi conferring low-level rifampin resistance through the ability to inactivate this antibiotic via its decomposition was identified. the iri (inactivation of rifampin) gene consisted of an open reading frame of 1,437 bp encoding a 479-amino-acid sequence strongly resembling those of monooxygenases acting upon phenolic compounds or involved in polyketide antibiotic synthesis. when expressed in escherichia coli, the gene conferred resistance to a > 50-micrograms/ml co ... | 1997 | 8980786 |
| characterization of three distinct extradiol dioxygenases involved in mineralization of dibenzofuran by terrabacter sp. strain dpo360. | the dibenzofuran-degrading bacterial strain dpo360 represents a new species of the genus terrabacter together with the previously described dibenzofuran-mineralizing bacterial strain dpo1361 (k.-h. engesser, v. strubel, k. christoglou, p. fischer, and h. g. rast, fems microbiol. lett. 65:205-210, 1989; v. strubel, ph.d. thesis, university of stuttgart, stuttgart, germany, 1991; v. strubel, h. g. rast, w. fietz, h.-j. knackmuss, and k.-h. engesser, fems microbiol. lett. 58:233-238, 1989). two 2,3 ... | 1997 | 8981980 |
| amide metabolism: a putative abc transporter in rhodococcus sp. r312. | the dna sequence has been determined upstream of the amie structural gene in the amidase operon of rhodococcus sp. r312 and a new orf (amis2) identified. the amis2 gene encodes a potential 206 amino acid (aa) protein containing a high proportion of hydrophobic residues. the amis2 protein possesses high homology to the orfp3, amis and urei gene products from the mycobacterium smegmatis (ms) acetamidase operon, pseudomonas aeruginosa (pa) amidase operon and helicobacter pylori (hp) urease operon, ... | 1996 | 8982091 |
| cytologic features of pulmonary malakoplakia related to rhodococcus equi in an immunocompromised host. | cytologic features are described in bronchial brushings of a large cavitary lung mass from an immunosuppressed patient who had undergone liver transplantation. scattered histiocytes with abundant eosinophilic, vacuolated cytoplasm were noted in a background of bronchial cells. within approximately one fourth of the histiocytes, targetoid intracytoplasmic inclusions were present, consistent with michaelis-gutmann bodies. they were diffusely positive by histo-chemical staining with von kossa, gomo ... | 1996 | 8982589 |
| identification of rhodococcus equi using the polymerase chain reaction. | two regions in the gene coding for 16s rrna in rhodococcus equi were selected as species-specific primer sequences for the polymerase chain reaction (pcr). pcr using these primers was tested against 10 strains of r. equi (including the type strain) and gave positive results for all but was negative for all other tested species of rhodococcus; representatives of the most closely related genera and a number of other bacterial species. this method could therefore be used to identify this species wh ... | 1996 | 8987445 |
| succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line u937 into monocyte-macrophages. | a novel type of succinoyl trehalose lipid (stl-1) prepared from n-hexadecane-culture of rhodococcus erythropolis sd-74 markedly inhibited the growth of a human monocytoid leukemic cell line, u937, and induced its morphological alteration along a monocyte-macrophage lineage. stl-1 markedly increased differention-associated characteristics in macrophage, such as nitroblue tetrazolium reducing ability, appearance of fc receptor, phagocytic activities in u937. furthermore, u937 cells, which were act ... | 1995 | 8987500 |
| nitrile hydratase and its application to industrial production of acrylamide. | nitrile hydratase (nhase) was discovered in our laboratory. this enzyme was purified and characterized from various microorganisms. nhases are roughly classified into two groups according to the metal involved: fe-type and co-type. nhases are expected to have great potential as catalysts in organic chemical processing because they can convert nitriles to the corresponding higher-value amides under mild conditions. we have used microbial enzymes for the production of useful compounds: nhase has b ... | 1996 | 8987584 |
| resonance raman evidence that photodissociation of nitric oxide from the non-heme iron center activates nitrile hydratase from rhodococcus sp. n-771. | nitrile hydratase (nhase) from rhodococcus sp. n-771, which contains a non-heme iron center in the catalytic site, has been known to be activated by light illumination. recently, endogenous nitric oxide (no) was found in this enzyme by ftir spectroscopy [noguchi et al. (1995) febs lett. 358, 9-12]. in order to directly detect the bonding between no and the iron atom and the reaction of no upon photoactivation, resonance raman spectra of the nhase were measured with 413 nm excitation at 85 k. two ... | 1996 | 8988015 |
| a novel transporter involved in cobalt uptake. | cobalt is an essential component of a low molecular-mass nitrile hydratase (l-nhase) from rhodococcus rhodochrous j1. we have found a new gene, nhlf, in the dna region sandwiched between nhlba encoding l-nhase and amda encoding amidase, which are involved in the degradation of nitriles. the product of nhlf, nhlf, shows a significant sequence similarity with those of hoxn from alcaligenes eutrophus, hupn from bradyrhizobium japonicum, nixa from helicobacter pylori, and ureh from bacillus sp., whi ... | 1997 | 8990157 |
| characterization of catechol catabolic genes from rhodococcus erythropolis 1cp. | the biochemical characterization of the muconate and the chloromuconate cycloisomerases of the chlorophenol-utilizing rhodococcus erythropolis strain 1cp previously indicated that efficient chloromuconate conversion among the gram-positive bacteria might have evolved independently of that among gram-negative bacteria. based on sequences of the n terminus and of tryptic peptides of the muconate cycloisomerase, a fragment of the corresponding gene has now been amplified and used as a probe for the ... | 1997 | 8990288 |
| cloning, analysis, and overexpression of the gene encoding isobutylamine n-hydroxylase from the valanimycin producer, streptomyces viridifaciens. | the flavoprotein isobutylamine n-hydroxylase (ibah) catalyzes the oxidation of isobutylamine to isobutylhydroxylamine, a key step in the biosynthesis of the azoxy antibiotic valanimycin. by using oligonucleotide primers designed from peptide sequence information derived from native ibah, a fragment of the gene (vlmh) encoding ibah was amplified by pcr from a genomic library of the valanimycin-producing organism, streptomyces viridifaciens mg456-hf10. the gene fragment was then employed as a prob ... | 1997 | 8990292 |
| [the degradation of plasticizers by rhodococcus erythropolis 40f]. | a rhodococcus erythropolis 40 phi strain isolated from the soil utilizes 0.1-2% v/v dibutylphthalate (dbp) and di-(2-ethylhexyl)-phthalate (dehp) as a single carbon source. the strain metabolizes dbp and dehp via the following steps: diester --> phthalate --> benzoate --> p-hydroxybenzoate --> 3,4-dihydroxybvenzoate --> 3-ketoadipinate. | 1996 | 8991593 |
| a proposal to reclassify nocardia pinensis blackall et al. as skermania piniformis gen. nov., comb. nov. | the type strain of nocardia pinensis was the subject of chemotaxonomic and 16s ribosomal dna sequencing studies. the resultant nucleotide sequence was aligned with the sequences of representatives of the genera corynebacterium, dietzia, gordona, mycobacterium, nocardia, rhodococcus, and tsukamurella, and phylogenetic trees were generated by using the fitch-margoliash, maximum-parsimony, maximum-likelihood, and neighbor-joining methods. it was evident from the phylogenetic analyses that n. pinens ... | 1997 | 8995814 |
| a phylogenetic analysis of aerobic polychlorinated biphenyl-degrading bacteria. | several bacterial isolates were characterized based on their abilities to degrade specific polychlorinated biphenyls (pcbs) and their 16s rrna gene sequences. the members of one group of bacteria consisting of alcaligenes species, including the pcb-degrading bacterium alcaligenes eutrophus h850, had strong abilities to degrade a broad range of pcbs but not the di-para-chlorine-substituted congeners. the members of another group, which included the pcb-degrading bacterium originally classified as ... | 1997 | 8995824 |
| subunit topology of the rhodococcus proteasome. | the 20s proteasome, isolated from the nocardioform actinomycete rhodococcus erythropolis strain ni86/21, is built from two alpha-type and two beta-type subunits. in order to probe the subunit topology, we have set up an expression system which allows coexpression of the genes encoding the alpha- and beta-subunits in all possible combinations. the four respective constructs obtained yielded fully assembled and proteolytically active proteasomes. biochemical, kinetic and electron microscopy analys ... | 1997 | 9000518 |
| biodegradation of polyhydroxyalkanoic acids. | stimulated by the commercial availability of bacteriologically produced polyesters such as poly[(r)-3-hydroxybutyric acid], and encouraged by the discovery of new constituents of polyhydroxyalkanoic acids (pha), a considerable body of knowledge on the metabolism of pha in microorganisms has accumulated. the objective of this essay is to give an overview on the biodegradation of pha. the following topics are discussed: (i) general considerations of pha degradation, (ii) methods for identification ... | 1996 | 9008883 |
| interpretation of chest radiographs in aids patients: usefulness of cd4 lymphocyte counts. | specific infections and neoplasms that are complications of acquired immunodeficiency syndrome (aids) occur within various cd4 lymphocyte count ranges. knowledge of how these counts correlate with radiographic appearances of these entities can limit the differential diagnosis because certain conditions are uncommon above a specific count. in patients with cd4 lymphocyte counts above 200 cells/mm3 and radiographic findings of cavitary and noncavitary consolidation, bacterial pneumonia and mycobac ... | 1997 | 9017798 |
| a flavin reductase stimulates dsza and dszc proteins of rhodococcus erythropolis igts8 in vitro. | rhodococcus erythropolis igts8 is a gram positive bacterium, which can catabolize dibenzothiophene to 2-hydroxybiphenyl and inorganic sulfur without the cleavage of carbon-carbon bonds. three structural genes, dsza, dszb, and dszc, have been cloned and shown to be necessary for this phenotype. here, we demonstrate that a fmn:nadph oxidoreductase from vibrio harveyi complements activities of purified dsza and dszc proteins. furthermore, we propose that dsza and dszc are oxygenase units that do no ... | 1997 | 9020064 |
| the plasmid-located haloalkane dehalogenase gene from rhodococcus rhodochrous ncimb 13064. | the haloalkane dehalogenase (dhaa) gene from rhodococcus rhodochrous ncimb 13064 was cloned and sequenced. its comparison with the previously studied dhla gene from xanthobacter autotrophicus gj10 did not show homology. however, the amino acid sequences of the products of these genes showed approximately 30% identity and several of the catalytic amino acid residues were conserved in the ncimb 13,064 dehalogenase. a high level of dhaa expression was demonstrated in escherichia coli cells and this ... | 1997 | 9025284 |
| [photoreactive nitrite hydratase]. | | 1997 | 9028170 |
| rhodococcus or mycobacterium? an example of misdiagnosis in hiv infection. | | 1997 | 9030376 |
| chronic pneumonia caused by rhodococcus equi in a patient without impaired immunity. | a 48 year old, human immunodeficiency virus (hiv)-negative, immunocompetent male patient had a chronic progressive pulmonary infiltrate, without radiological cavitation, in the middle lobe of the right lung produced by rhodococcus equi. he reported direct contact with a diseased dog. the patient was diagnosed by thoracotomy and treated by lobectomy. after 16 months of follow-up, the patient was asymptomatic and had neither recurrence nor immunological disturbances. | 1997 | 9032523 |
| classification of catechol 1,2-dioxygenase family: sequence analysis of a gene for the catechol 1,2-dioxygenase showing high specificity for methylcatechols from gram+ aniline-assimilating rhodococcus erythropolis an-13. | gram+ aniline-assimilating rhodococcus erythropolis an-13 (an-13) produces catechol 1,2-dioxygenase (c12o) showing high enzymatic activities for 3- and 4-methylcatechols [aoki et al. (1984) agric. biol. chem. 48, 2087-2095]. a 3.0 kb sau3ai fragment carrying a gene encoding c12o(cata) was cloned by selection of transformants showing c12o activity from a gene library of an-13. furthermore, we specified a 1.6 kb sali fragment containing cata from the sau3ai fragment by subcloning. sequence analysi ... | 1997 | 9034312 |
| biodegradation of methyl t-butyl ether by pure bacterial cultures. | three pure bacterial cultures degrading methyl t-butyl ether (mtbe) were isolated from activated sludge and fruit of the gingko tree. they have been classified as belonging to the genuses methylobacterium, rhodococcus, and arthrobacter. these cultures degraded 60 ppm mtbe in 1-2 weeks of incubation at 23-25 degrees c. the growth of the isolates on mtbe as sole carbon source is very slow compared with growth on nutrient-rich medium. uniformly-labeled [14c]mtbe was used to determine 14co2 evolutio ... | 1997 | 9035411 |
| identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. | coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ... | 1997 | 9039559 |
| restriction enzyme analysis of the virulence plasmids of vapa-positive rhodococcus equi strains isolated from humans and horses. | restriction enzyme digestion patterns of the large virulence plasmids of 8 human and 37 foal isolates of virulence-associated protein (vapa)-positive rhodococcus equi strains from different sources were compared. foal isolates came from five continents. digestion with ecori divided these plasmids into three closely related types, and digestion with bamhi divided them into three major types which corresponded to the ecori types. the only ecori and bamhi type 3 plasmid was from a single foal isola ... | 1997 | 9041424 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22 | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9042758 |
| equine herpesvirus type 2: prevalence and seroepidemiology in foals. | whole blood and serum were collected from foals to determine the prevalence of equine herpesvirus type 2 (ehv 2) infection in foals, age at which infection can first be identified and serological responses to infection. equine herpesvirus type 2 was isolated from peripheral blood mononuclear cells (pbmc) from 68 of 69 foals, 1-8-months-old, sampled once. virus isolation was performed twice at intervals of 2-7 months on pbmcs from 33 foals and ehv2 was isolated on both occasions in all but one fo ... | 1996 | 9049491 |
| radiologic features of rhodococcus equi pneumonia in aids. | this report outlines the radiological features observed in three cases of rhodococcus equi (r. equi) pneumonia in aids (acquired immunodeficiency syndrome) and reviews another 45 radiological reports published of this emerging opportunistic pneumonia in human immunodeficiency virus (hiv) infected patients. the clinical signs in our three patients consisted in a subacute onset of respiratory symptoms and fever. a low lymphocyte count (< 200 cells/mm3), pulmonary infiltrates, and pleural effusion ... | 1997 | 9056153 |
| bacterial degradation of emulsified crude oil and the effect of various surfactants. | a rhodococcus sp. 094 bacterium was tested for its ability to oxidize alkanes in crude oil emulsified by nonionic chemical and biological surfactants. oxidation rates were measured in a 3-h period by warburg respirometry. 14co2 recovery was measured from the [1-14c]hexadecane spiked crude oil. response to emulsified oil depended on the physiological state of the bacteria (i.e., cells harvested in the exponential and stationary growth phases) were tested. oxidation rates by cells in the exponenti ... | 1997 | 9057292 |
| heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products. | the bpha1a2a3a4 gene cluster, encoding a biphenyl dioxygenase from rhodococcus globerulus p6, a gram-positive microorganism able to degrade a wide spectrum of polychlorobiphenyls (pcbs), was expressed in pseudomonas putida, thereby allowing characterization of chlorobiphenyl oxidation by this enzyme. while p6 biphenyl dioxygenase activity was observed in p. putida containing bpha1a2a3a4, no activity was detected in escherichia coli cells containing the same gene cluster. in e. coli, transcriptio ... | 1997 | 9068637 |
| [structure and antigenicity of the major glycolipid from opportunistic bacteria rhodococcus equi]. | some results from structural and immunological studies of the major glycolipid from rhodococcus equi were presented. this glycolipid showed to be a glucosylmonomycolate (gmm) with an aliphatic chain c38. its value as taxonomic and immunodiagnostic marker was discussed. | 1996 | 9072772 |
| the bphdef meta-cleavage pathway genes involved in biphenyl/polychlorinated biphenyl degradation are located on a linear plasmid and separated from the initial bphacb genes in rhodococcus sp. strain rha1. | the bphacb genes responsible for the initial oxidation of the aromatic ring of biphenyl/polychlorinated biphenyls (pcb) to meta-cleavage product in rhodococcus sp. rha1 have been characterized. we cloned the 6.1 kb ecori fragment containing another extradiol dioxygenase gene (etbc) which was induced during the growth on ethylbenzene. the bphd, bphe and bphf encoding 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopd) hydrolase, 2-hydroxypenta-2,4-dienoate hydratase and 4-hydroxy-2-oxovalerate aldol ... | 1997 | 9073078 |
| differentiation of human promyelocytic leukemia cell line hl60 by microbial extracellular glycolipids. | microbial extracellular glycolipids, succinoyl trehalose lipid (stl), and mannosylerythritol lipid (mel) inhibited the growth of a human promyelocytic leukemia cell line, hl60, and induced their morphological changes. the results of specific and nonspecific leukocyte esterase activities showed that stl induced monocytotic differentiation while mel induced granulocytic differentiation. stl and mel markedly increased common differentiation-associated characteristics in monocytes and granulocytes, ... | 1997 | 9076663 |
| acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes. | aerobic acetylene-degrading bacteria were isolated from soil samples. two isolates were assigned to the species rhodococcus opacus, two others to rhodococcus ruber and gordona sp. they were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. the acetylene hydratases of r opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(iii) citrate. expression of these enzymes was molybdenum-dependent. acetylene hydrat ... | 1997 | 9084145 |
| long survival of a patient with aids in spite of previous rhodococcus equi infection. | | 1997 | 9085620 |
| bacterial infections. | non-opportunistic bacterial infections are an important cause of morbidity and mortality for hiv-infected adults and children. factors associated with increased risk of these include altered b- and t-cell function; altered phagocytic cell function; skin and mucous membrane defects; and use of indwelling vascular catheters, antibiotics, or cytotoxic agents. the pathogens encountered most frequently are s. aureus, s. pneumoniae, h. influenzae, salmonella sp., and pseudomonas aeruginosa. less commo ... | 1997 | 9093231 |
| prevention of insect-borne disease: an approach using transgenic symbiotic bacteria. | expression of molecules with antiparasitic activity by genetically transformed symbiotic bacteria of disease-transmitting insects may serve as a powerful approach to control certain arthropod-borne diseases. the endosymbiont of the chagas disease vector, rhodnius prolixus, has been transformed to express cecropin a, a peptide lethal to the parasite, trypanosoma cruzi. in insects carrying the transformed bacteria, cecropin a expression results in elimination or reduction in number of t. cruzi. a ... | 1997 | 9096383 |
| rhodococcus equi pneumonia. | corynebacterium equi is a pleomorphic gram-positive rod that was first isolated in 1923 by magnusson, and is the cause of suppurative broncho-pneumonia in foals. the organism, now know as rhodococcus equi, is ubiquitous in nature and is increasingly recognized as pathogenic, particularly in the immunocompromised population. cavitary pneumonia clinically resembling mycobacterium tuberculosis is the most common manifestation of r equi disease, but extrapulmonary infections are seen as well. long t ... | 1997 | 9097379 |
| characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/polychlorobiphenyl-degrading bacterium, rhodococcus sp. strain m5. | we report the cloning, sequence, and expression of the bpds and bpdt genes from rhodococcus sp. strain m5, which are believed to encode the first two-component signal transduction system in the genus rhodococcus, which potentially regulates biphenyl/polychlorobiphenyl metabolism in m5. bpdt has a typical responses regulator sequence (209 amino acids; 23 kda), whereas bpds, the predicted histidine kinase component, is an unusually large transmembrane protein (1,576 amino acids; 170 kda) that cont ... | 1997 | 9098081 |
| use of rhodococcus equi virulence-associated protein for immunization of foals against r equi pneumonia. | to evaluate use of the virulence-associated protein of rhodococcus equi in immunizing foals against r equi pneumonia. | 1997 | 9099378 |
| pulmonary malacoplakia associated with rhodococcus equi infection in an aids patient. report of a case with diagnosis by fine needle aspiration. | malacoplakia is an uncommon, peculiar inflammatory disease with few cytologic descriptions. it is seen mostly in the lower urinary tract, with rare occurrences in the lungs. escherichia coli is the most common underlying etiologic agent, with only a few cases reported with rhodococcus equi infection. | 1997 | 9100789 |
| rhodococcus equi infections: antibiotic therapy and relapses. | | 1997 | 9110086 |
| strategy for detection and identification of bacteria based on 16s rrna genes in suspected cases of whipple's disease. | the 16s ribosomal rna (rrna) gene of the phylogenetic subdivision containing gram-positive bacteria with a high g + c content was detected specifically in clinical specimens from patients suspected of having whipple's disease. the primary structure of 16s rdna amplified from clinical samples was determined by cloning and sequencing. two sorts of sequences were identified: one corresponded exactly to the rrna sequence of tropheryma whippelii (genbank accession no. m87484) while the other was rela ... | 1997 | 9128199 |
| pathogenetic role of phagocytic abnormalities in human virus immunodeficiency infection: possible therapeutical approaches. a review. | polymorphonuclear cells (pmn) and monocytes/macrophages (m/m) represent the first defence line against invading microorganisms. both phagocytic cell functions are precociously compromised in human immunodeficiency virus (hiv)-infected subjects, thus leading to infectious and neurological complications in the late stages of disease. among intracellular pathogens, emerging bacteria such as bartonella henselae and rhodococcus equi can cause peculiar clinical pictures, i.e. the bacillary parenchimal ... | 1997 | 9130002 |
| acute mediastinitis due to rhodococcus equi in a patient with human immunodeficiency virus infection. | a patient with human immunodeficiency virus infection developed anterior mediastinitis during antibiotic treatment for empyema due to rhodococcus equi. this is the first reported case of infection with this organism in this setting. despite an adequate course of therapy and maintenance treatment with antibiotics to which the isolate of rhodococcus equi was susceptible in vitro, the patient experienced relapse of the infection into the mediastinum. clinicians should consider this complication whe ... | 1997 | 9131329 |
| location, catalytic activity, and subunit composition of nad-reducing hydrogenases of some alcaligenes strains and rhodococcus opacus mr22. | six new strains of alcaligenes enriched for and isolated as nickel-resistant bacteria resemble alcaligenes eutrophus h16 and contain both an nad-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. none of the soluble hydrogenases share with the rhodococcus opacus mr11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (betadelta) and an nadh:acceptor oxidoreductase (alphagamma)], in the absence of nickel or at low ionic strength. ... | 1997 | 9133325 |
| [characterization of rhodococcus equi isolates from horse and man]. | in the present investigation 19 and 22 r. equi-cultures isolated from diseased horses and humans, respectively, could be correctly identified by their morphological, biochemical and serological properties. the rod-coccus life cycle appeared to be a common feature of almost all cultures investigated. the cultures were typeable with the typing system described by prescott (1981). the predominant serotypes among the r. equi-isolates belonged to serotypes 1 and 2. among the r. equi-isolates from hor ... | 1997 | 9139629 |
| genes encoding the nad-reducing hydrogenase of rhodococcus opacus mr11. | the dissociation of the soluble nad-reducing hydrogenase of rhodococcus opacus mr11 into two dimeric proteins with different catalytic activities and cofactor composition is unique among the nad-reducing hydrogenases studied so far. the genes of the soluble hydrogenase were localized on a 7.4 kbp asnl fragment of the linear plasmid phg201 via heterologous hybridization. analysis of the nucleotide sequence of this fragment revealed the seven open reading frames orf1, hoxf, -u, -y, -h, -w and orf7 ... | 1997 | 9141690 |
| thiocarbamate herbicide-inducible nonheme haloperoxidase of rhodococcus erythropolis ni86/21. | during biodegradation of thiocarbamate herbicides by rhodococcus erythropolis ni86/21, a protein with an m(r) of 30,000 is induced (i. nagy, g. schoofs, f. compernolle, p. proost, j. vanderleyden, and r.de mot, j. bacteriol. 177:676-687, 1995). based on n-terminal sequence data for the protein purified by two-dimensional electrophoresis, the corresponding structural gene, thcf, was cloned and sequenced. the deduced protein sequence of thcf is homologous to those of nonheme haloperoxidases. a par ... | 1997 | 9143122 |
| molecular analysis of the rhodococcus sp. strain h1 her gene and characterization of its product, a heroin esterase, expressed in escherichia coli. | the structural gene for heroin esterase was cloned from rhodococcus sp. strain h1 and expressed in escherichia coli bl21(de3). the purified enzyme was found to be a tetramer with an m(r) of 137,000 and an apparent k(m) of 0.88 mm for 6-acetylmorphine. the g-x-s-x-g motif was observed in the deduced amino acid sequence, suggesting that the enzyme is a serin esterase. | 1997 | 9143135 |
| virulent and avirulent rhodococcus equi infection in t-cell deficient athymic nude mice: pathologic, bacteriologic and immunologic responses. | we investigated the pathologic, bacteriologic and immunologic responses of balb/c-nu/nu mice (nude mice) and balb/c mice (euthymic mice) infected intravenously with virulent and avirulent rhodococcus equi atcc 33701, and its plasmid-cured derivative atcc 33701p-, to evaluate the role of t lymphocytes. adaptive transfer of immune and normal spleen cells into nude mice was also investigated. nude and euthymic mice were inoculated with 10(6) atcc 33701 or 10(6) atcc 33701p- intravenously (i.v.) and ... | 1997 | 9143883 |