| beta-lactamases as fully efficient enzymes. determination of all the rate constants in the acyl-enzyme mechanism. | the rate constants for both acylation and deacylation of beta-lactamase pc1 from staphylococcus aureus and the rtem beta-lactamase from escherichia coli were determined by the acid-quench method [martin & waley (1988) biochem. j. 254, 923-925] with several good substrates, and, for a wider range of substrates, of beta-lactamase i from bacillus cereus. the values of the acylation and deacylation rate constants for benzylpenicillin were approximately the same (i.e. differing by no more than 2-fold ... | 1990 | 2158301 |
| phospholipids chiral at phosphorus. stereochemical mechanism of reactions catalyzed by phosphatidylinositide-specific phospholipase c from bacillus cereus and guinea pig uterus. | (rp)- and (sp)-1,2-dipalmitoyl-sn-glycero-3-thiophosphoinositol (dppsi) were synthesized as a mixture and their configurations assigned on the basis of the stereospecific hydrolysis catalyzed by phospholipase a2 (pla2) from bee venom. pla2 is known to be stereospecific to the rp isomer of 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) and 1,2-dipalmitoyl-sn-glycero-3-thiophosphoethanolamine (dppse). since the configurations of (rp)- and (sp)-dppsi correspond to those of (sp)- and (rp)-d ... | 1990 | 2161255 |
| physical map of the bacillus cereus chromosome. | a physical map of the bacillus cereus chromosome has been constructed by aligning 11 noti fragments, ranging in size from 200 to 1,300 kilobases. the size of the chromosome is about 5.7 megabases. this is the first bacillus genome of which a complete physical map has been described. | 1990 | 2163389 |
| molecular analysis of a sphingomyelinase c gene from leptospira interrogans serovar hardjo. | a thermolabile hemolysin from leptospira interrogans serovar hardjo, strain sponselee, was shown to specifically degrade sphingomyelin. nucleotide sequence determination revealed that sphingomyelinase activity was encoded by an open reading frame of 1,668 nucleotides. although a putative signal sequence could be identified, no evidence for protein export in either l. interrogans or escherichia coli was obtained. the apparent molecular mass of the expression product in e. coli minicells was 41.2 ... | 1990 | 2163985 |
| phosphatidylinositol-specific phospholipase c from bacillus cereus combines intrinsic phosphotransferase and cyclic phosphodiesterase activities: a 31p nmr study. | the inositol phosphate products formed during the cleavage of phosphatidylinositol by phosphatidylinositol-specific phospholipase c from bacillus cereus were analyzed by 31p nmr. 31p nmr spectroscopy can distinguish between the inositol phosphate species and phosphatidylinositol. chemical shift values (with reference to phosphoric acid) observed are 0.41, 3.62, 4.45, and 16.30 ppm for phosphatidylinositol, myo-inositol 1-monophosphate, myo-inositol 2-monophosphate, and myo-inositol 1,2-cyclic mo ... | 1990 | 2175645 |
| determination of phosphatidylcholine in a flow injection system using immobilized enzyme reactors. | two alternative procedures are described for the quantitative determination of phosphatidylcholine in a flow-injection system utilizing immobilized enzymes. phospholipase c from bacillus cereus and phospholipase d from cabbage were covalently bound to the surface of controlled-pore glass beads and the enzyme-derivatized beads were packed in small columns. in the first procedure, the phospholipase c column was connected with a second column containing coimmobilized alkaline phosphatase and cholin ... | 1990 | 2200305 |
| isolation and characterization of a beta-lactamase-inhibitory protein from streptomyces clavuligerus and cloning and analysis of the corresponding gene. | culture filtrates of streptomyces clavuligerus contain a proteinaceous beta-lactamase inhibitor (blip) in addition to a variety of beta-lactam compounds. blip was first detected by its ability to inhibit bactopenase, a penicillinase derived from bacillus cereus, but it has also been shown to inhibit the plasmid puc- and chromosomally mediated beta-lactamases of escherichia coli. blip showed no inhibitory effect against enterobacter cloacae beta-lactamase, and it also showed no activity against a ... | 1990 | 2203736 |
| expression of the blasticidin s deaminase gene (bsr) in tobacco: fungicide tolerance and a new selective marker for transgenic plants. | blasticidin s (bs), a fungicide of microbial origin, is used for the practical control of rice blast disease. it has broad antimicrobial activity but occasionally exhibits adverse phytotoxic effects on some dicot plants. an inactivating enzyme, bs deaminase, was discovered in the bs resistant strain, bacillus cereus k55-s1, and the structural gene, bsr, for the enzyme has been cloned. we introduced the bsr gene into tobacco plants using the ti plasmid vector system and demonstrated that the bsr ... | 1990 | 2250657 |
| asymmetric short-chain phosphatidylcholines: defining chain binding constraints in phospholipases. | several short-chain asymmetric lecithins with a total of 14 carbons in the acyl chains (ranging from 1-lauroyl-2-acetylphosphatidylcholine to 1-hexanoyl-2-octanoylphosphatidylcholine) have been synthesized and characterized. the specific activities of phospholipase a2 from cobra venom, phospholipase a2 from porcine pancreas, and phospholipase c from bacillus cereus toward these lecithins as micelles have been determined. the results of these kinetic studies allow the definition of hydrophobic bi ... | 1990 | 2271632 |
| toxicological, nutritional and microbiological evaluation of tempe fermentation with rhizopus oligosporus of bitter and sweet apricot seeds. | bitter and sweet apricot seeds are by-products of the apricot processing industry. bitter seeds, in particular, contain toxic levels of the cyanogenic substance amygdalin. tempe was made from both kinds of seeds. the bitter seeds contain antimicrobial substances which must be removed by leaching and boiling prior to tempe fermentation. apricot seed tempe had an agreeable taste. it contained approx. 21% (w/w) crude protein, 52% (w/w) crude fat, 1.5% (w/w) crude fibre and 25.5% (w/w) carbohydrates ... | 1990 | 2282289 |
| rapid methods for differentiating gram-positive from gram-negative aerobic and facultative anaerobic bacteria. | different tests based on lysis by koh and on reaction with fluorogenic and chromogenic substrates, l-alanine-4-nitroanilide (lana); l-alanine-4-methoxy- beta-naphthylamide (mna); 4-alanine-2-amidoacridone (aaa); l-alanine-7-amido- 4-methylcoumarin (aamc); 8-anilino-1-naphthalene-sulphonic acid (ans) were compared for their suitability to distinguish gram-positive from gram-negative bacteria. a concentration of 100 micrograms/ml was chosen for incorporating lana, aaa, aamc and ans into the growth ... | 1990 | 2286581 |
| bacterial aetiology of infantile diarrhoea in papua new guinea. | bacterial examination of stools of children with diarrhoea was carried out at port moresby, papua new guinea, where little information is available concerning the causative bacteria of diarrhoea. shigellae, enterotoxigenic escherichia coli, bacillus cereus, staphylococcus aureus, campylobacter jejuni, and aeromonas hydrophila were isolated with the decreasing frequency in that order. among these, enterotoxigenic e. coli and c. jejuni were isolated for the first time in this country. although vib ... | 1990 | 2362308 |
| sigma 29-like protein is a common sporulation-specific element in bacteria of the genus bacillus. | a monoclonal antibody specific for an antigenic determinant on the bacillus subtilis sporulation-induced sigma factor sigma 29 reacted with proteins similar in size to sigma 29 in extracts of sporulating bacillus licheniformis, bacillus amyloliquifaciens, bacillus cereus, bacillus natto, and bacillus pumilus but not in extracts prepared from vegetatively growing cultures of these bacteria. these results indicate that rna polymerase modifications, initially described for b. subtilis, are likely t ... | 1985 | 2415506 |
| the membrane-anchoring systems of vertebrate acetylcholinesterase and variant surface glycoproteins of african trypanosomes share a common antigenic determinant. | amphiphilic detergent-soluble acetylcholinesterase (ache) from torpedo is converted to a hydrophilic form by digestion with phospholipase c from trypanosoma brucei or from bacillus cereus. this lipase digestion uncovers an immunological determinant which crossreacts with a complex carbohydrate structure present in the hydrophilic form of all variant surface glycoproteins (vsg) of t. brucei. this crossreacting determinant is also detected in human erythrocyte ache after digestion with t. brucei l ... | 1986 | 2422055 |
| sporulation-dependent phagelike particles in inclusion-forming bacillus species. | phagelike particles, which have been observed in sporulated cells of bacillus medusa, were found to appear at about late stage ii in all subspecies of bacillus thuringiensis toxic to mosquito larvae. all phagelike particles were similar in size and shape and when purified possessed capsid proteins of similar molecular weights and immune reaction. the synthesis of the major capsid protein occurred at the same time as complete phagelike particles were detected by electron microscopy. the productio ... | 1986 | 2424582 |
| in vivo effect of lipoteichoic acid on biochemical function of peritoneal macrophages. | | 1986 | 2426192 |
| release of proteins from the surface of bovine central nervous system myelin by salts and phospholipases. | incubation of bovine cns myelin with phospholipase c from bacillus cereus under conditions that lead to extensive phospholipid degradation caused 10% of the myelin protein to be released from the membrane. the myelin basic protein (mbp) was a major component of the dissolved protein. comparable incubations with phospholipase c from clostridium perfringens, phosphatidylinositol-specific phospholipase c from staphylococcus aureus, or cabbage phospholipase d removed little mbp. however, concentrati ... | 1988 | 2448423 |
| improvement of beta-amylase production by new beta-amylase producers and new culture conditions. | | 1987 | 2449113 |
| comparative 16s rrna oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives. | the phylogenetic incoherency of the genus bacillus as presently described is demonstrated by analysis of both published and new data from comparative 16s rrna cataloguing of nine bacillus species and a number of related non-bacillus taxa, i.e. caryophanon latum, filibacter limicola and planococcus citreus. while the ellipsoidal-spore-forming bacilli, e.g. b. subtilis and allied species, formed a coherent cluster, the round-spore-forming bacilli showed a higher degree of relationship to the non-s ... | 1987 | 2452227 |
| [polyvinylpyrrolidone as a protective agent during the drying of bacillus cereus by the sublimation method]. | | 1988 | 2452296 |
| bacereutin, an antifungal antibiotic isolated from metabolites of bacillus cereus chu 130. | an antifungal metabolite, bacereutin, was isolated from culture filtrate of bacillus cereus chu 130. the bacterium was isolated from soils collected in changhwa county, taiwan, and was grown in soybean meal-mannitol broth for production of the antibiotic metabolite. the antibiotic metabolite was isolated by adsorption column chromatography of amberite xad-2 and was purified by passing through the chromatographic columns packed with dowex 50w-x8, sephadex lh 20 and biogel p-2. the antibiotic meta ... | 1989 | 2484044 |
| bacillus cereus panophthalmitis associated with intraocular gas bubble. | it has become increasingly apparent that bacillus cereus can cause a severe and devastating form of endophthalmitis following penetrating trauma by a metallic object. b. cereus is an uncommon aetiological agent in non-clostridial gas-forming infections. the patient studied in this single case report showed evidence of intraocular gas mimicking gas gangrene infection. the physiology of non-clostridial bacteria producing gas from anaerobic metabolic conditions is reviewed. further intraocular and ... | 1989 | 2493262 |
| high-resolution (1.5 a) crystal structure of phospholipase c from bacillus cereus. | both the phosphatidylinositol-hydrolysing and the phosphatidylcholine-hydrolysing phospholipases c have been implicated in the generation of second messengers in mammalian cells. the phosphatidylcholine-hydrolysing phospholipase c (plc) from bacillus cereus, a monomeric protein containing 245 amino-acid residues, is similar to some of the corresponding mammalian proteins. this, together with the fact that the bacterial enzyme can mimic the action of mammalian plc in causing, for example, enhance ... | 1989 | 2493587 |
| the migration of bacteria through gels in the presence of iucd monofilament tails. | an in vitro model was developed to investigate the migration of a variety of bacteria of different characteristics through a gel system in the presence or absence of a wide range of polymer monofilament threads. the bacteria were unable to migrate through the gel from the point of inoculation in the absence of a solid substrate. migration occurred along all thread types tested, including those used as iucd marker tails and the extent of bacterial migration appeared to be determined primarily by ... | 1989 | 2495894 |
| phosphatidylinositol-specific phospholipase c from bacillus cereus: improved purification, amino acid composition, and amino-terminal sequence. | phosphatidylinositol-specific phospholipase c was purified in a 27% yield from the culture medium of bacillus cereus by a combination of ammonium sulfate precipitation and ion-exchange and hydrophobic interaction chromatography. the purified enzyme was free of other phospholipase c-type activities and exhibited a high specific activity of approximately 1,300 units/mg. amino acid composition analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated a molecular weight of abo ... | 1989 | 2496136 |
| mechanism of inactivation of alanine racemase by beta, beta, beta-trifluoroalanine. | the alanine racemases are a group of plp-dependent bacterial enzymes that catalyze the racemization of alanine, providing d-alanine for cell wall synthesis. inactivation of the alanine racemases from the gram-negative organism salmonella typhimurium and gram-positive organism bacillus stearothermophilus with beta, beta, beta-trifluoroalanine has been studied. the inactivation occurs with the same rate constant as that for formation of a broad 460-490-nm chromophore. loss of two fluoride ions per ... | 1989 | 2496744 |
| conformational states of beta-lactamase: molten-globule states at acidic and alkaline ph with high salt. | we present evidence that beta-lactamase is close to fully unfolded (i.e., random coil conformation) at low ionic strength at the extremes of ph and that the presence of salt causes a cooperative transition to a conformation with the properties of a molten globule, namely, a compact state with native-like secondary structure but disordered side chains (tertiary structure). the conformation of beta-lactamase i from bacillus cereus was examined over the ph 1.5-12.5 region by circular dichroism (cd) ... | 1989 | 2496758 |
| [isolation, purification and identification of protective substances from a culture broth of germinating bacillus cereus spores]. | a fraction increasing the resistance of resting spores to uv-irradiation and high temperature has been isolated from the culture medium at the stage of b. cereus st. 96 spore initiation. amino acid analysis, gas chromatography, electrophoresis, and tlc of the products of acidic and alkaline hydrolysis of the isolated fraction demonstrated that the active component of the fraction was the lipoteichoic acid. | 1989 | 2497164 |
| survival of beta-lactamase-producing and -nonproducing bacteria in intravenous solutions. | the survival of beta-lactamase-producing (beta-lac+) and non-beta-lactamase-producing (beta-lac-) bacillus and staphylococcus spp. has been investigated in dextrose 5% injection, nacl 0.9%, and dextrose 5% in nacl 0.9% solutions. tests were performed under static and turbulent conditions of incubation, with and without antibiotics added to the fluids, and with or without 1% citrated blood. all solutions were inoculated with about 1000 organisms/ml, and sampled for viable bacteria at specific tim ... | 1989 | 2497589 |
| [change in the ultrastructure of the cell wall of bacillus cereus, determined by rp4 mucts62 plasmid]. | the influence of plasmid rp4 mucts62, heterologous for b. cereus, on the growth rate of b. cereus strains ga 682 and 319 obtained in our earlier experiments and on changes in the ultrastructure of their cell walls in comparison with b. cereus initial strains gp 7 and dsm 318 has been studied. plasmid rp4 mucts62 with a wide spectrum of action has been found not only to determine the functional signs of resistance to antibiotics and thermal sensitivity in the heterologous host, but also to take p ... | 1989 | 2499144 |
| a thiono-beta-lactam substrate for the beta-lactamase ii of bacillus cereus. evidence for direct interaction between the essential metal ion and substrate. | an 8-thionocephalosporin was shown to be a substrate of the beta-lactamase ii of bacillus cereus, a zinc metalloenzyme. although it is a poorer substrate, as judged by the kcat./km parameter, than the corresponding 8-oxocephalosporin, the discrimination against sulphur decreased when the bivalent metal ion in the enzyme active site was varied in the order mn2+ (the manganese enzyme catalysed the hydrolysis of the oxo compound but not that of the thiono compound), zn2+, co2+ and cd2+. this result ... | 1989 | 2499308 |
| bacillus cereus septicemia associated with rhabdomyolysis and myoglobinuric renal failure. | a patient with acute myeloblastic leukemia (aml) developed septicemia due to bacillus cereus with subsequent rhabdomyolysis and myoglobinuric renal failure. he died despite intensive care. postmortem examination revealed diffuse muscle necrosis with infiltration of gram-positive bacilli and widespread bacterial microthrombi in various organs. septicemia associated with rhabdomyolysis has been described in 12 cases. this case represents the first reported case of b. cereus septicemia associated r ... | 1989 | 2499725 |
| clinical significance of bacillus species isolated from blood cultures. | to determine the clinical significance of blood isolates of bacillus, we reviewed all blood cultures obtained at north carolina memorial hospital between 1981 and 1985. over the five-year study period the number of patients (incidence per 10,000 hospital admissions) from whom bacillus was isolated increased from 4.97 in 1981 to 12.5 in 1985. the incidence per 1,000 blood cultures also increased from 1.12 in 1981 to 2.33 in 1985. review of the medical records of 78 of the 95 patients (82%) with p ... | 1989 | 2499933 |
| development of a fluorescent immunodot assay for bacillus cereus enterotoxin. | a fluorescent immunodot assay has been developed for rapid, specific detection of b. cereus enterotoxin. none of the other bacillus species tested showed cross-reactivity in the assay with antiserum to purified b. cereus enterotoxin. the assay can detect greater than or equal to 50 ng of purified enterotoxin. using this assay system, enterotoxin was found to be produced by 25 of 25 foodborne disease-related isolates and 22 of 25 isolates not related to foodborne disease (isolates from routine su ... | 1989 | 2500487 |
| [septic pneumonia caused by bacillus cereus]. | the authors describe the case of a 3-year old girl whose septic pneumonia was caused by b. cereus and developed following serial viral and bacterial infections, which produced assumably transient immunoppressed condition. attention is drawn to the possibility of serious diseases caused by faculative bacteria in certain cases. the increasing pathological significance of bacteria existing in the soil is pointed out and the importance of hemoculture examination is stressed. | 1989 | 2500633 |
| mutations affecting the catalytic activity of bacillus cereus 5/b/6 beta-lactamase ii. | random in vitro mutagenesis of a cloned bacillus cereus 5/b/6 beta-lactamase ii gene was used to select defective genes unable to confer ampicillin or cephalosporin c resistance to escherichia coli. dna sequencing of mutant genes identified histidine at position 28 as important to beta-lactamase ii function. in addition, the isolation of six identical frameshift mutants established that the carboxyl-terminal end of beta-lactamase ii is critical for enzyme function. random mutagenesis also reveal ... | 1989 | 2501295 |
| microbiological quality and public health significance of ice-cream. | fifty samples of ice-cream were subjected to microbiological examination. of these 72% had total viable count over 10(6)/g while 66% had coliform count between 10(2)-10(3)/g. the micro-organisms isolated were escherichia coli (46%), enterobacter aerogenes (34%), staphylococcus aureus (26%), proteus species (16%), streptococcus faecalis (12%), citrobacter species (10%) and bacillus cereus (4%). the results showed that commercially prepared ice-cream sold in the market was not satisfactory for hum ... | 1989 | 2501524 |
| pseudomonas aeruginosa cytotoxin: the influence of sphingomyelin on binding and cation permeability increase in mammalian erythrocytes. | a cytotoxic protein isolated from pseudomonas aeruginosa damages the plasma membranes of many mammalian cells by forming pores. we studied binding of the 125i-cytotoxin and the resulting increase of cation permeability in erythrocytes of various mammalian species. the sensitivity of red blood cells was inversely related to the relative sphingomyelin content in their external surface. thus, erythrocytes with a sphingomyelin to phosphatidylcholine ratio below 1 (dog, rat, rabbit and man) were sens ... | 1989 | 2501911 |
| phospholipase c. | | 1989 | 2502447 |
| identification of bacillus anthracis by polyclonal antibodies against extracted vegetative cell antigens. | the extractable protein antigens ea1 and ea2 of bacillus anthracis were prepared from electrophoresis transblots of sds extracts of vegetative bacteria of the sterne strain. hyperimmune guinea-pig antiserum against ea2 failed to react with b. anthracis cells in immunofluorescence (if) tests. guinea-pig antiserum against ea1 (anti-ea1) reacted strongly in if tests with non-encapsulated vegetative cell of 10 of 12 strains of b. anthracis and with cells of strains of b. cereus and b. thuringiensis. ... | 1989 | 2502530 |
| [comparison of the sporocidal effects of two different disinfectants i a model experiment with pathogenic and nonpathogenic clostridial spore as well as with bacillus cereus]. | official methods concerning the examination of sporocidal effects of disinfectants are presently lacking in the federal republic of germany. spore resistance of selected clostridial species towards formaldehyde and peracetic acid was examined by suspension tests and germ-carrier tests in comparison to spores of bacillus cereus. formaldehyde in concentrations between 5% and 20% as well as peracetic acid in concentrations between .004% and .125% were tested. spore concentration of 10(6) spores/ml ... | 1989 | 2502997 |
| transformation of bacillus cereus vegetative cells by electroporation. | transformation of untreated vegetative cells of bacillus cereus 569 with plasmid pc194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. transformation of a 130-megadalton plasmid occurred at a comparable frequency. the method was simple, rapid, and yielded transformant colonies in 14 to 24 h. transformation was obtained with unpurified total plasmid dna. | 1989 | 2504113 |
| phosphonoacetaldehyde hydrolase from pseudomonas aeruginosa: purification properties and comparison with bacillus cereus enzyme. | phosphonoacetaldehyde hydrolase (2-oxoethylphosphonate phosphonohydrolase, ec 3.11.1.1) has been purified to electrophoretic homogeneity from cells of pseudomonas aeruginosa a 237 grown in a culture medium containing 2-aminoethylphosphonate as both phosphorus and carbon sources. the native mr has been estimated to be 62,000 +/- 2000, using a gel filtration column equilibrated with standard proteins. a subunit of mr 30,000 +/- 1000 determined in sodium dodecyl sulfate-polyacrylamide gel electroph ... | 1989 | 2504289 |
| studies on the cellular defense reactions of the madeira cockroach, leucophaea maderae: nodule formation in response to injected bacteria. | nodules were formed in the madeira cockroach, leucophaea maderae, in response to injections of low doses (3 x 10(4) bacteria/insect) of three strains of bacillus cereus and escherichia coli k12 d31. the most pathogenic strain of bacteria used, b. cereus b1, produced the greatest cellular response, while the least pathogenic, e. coli k12 d31, injected at the same dose, caused little nodule formation. similarly, nodules were generally found to be larger following injection of pathogenic bacteria s ... | 1989 | 2504818 |
| bacillus species infections in neonates. | a case of neonatal meningitis due to bacillus cereus is presented. postmortem histopathologic examination revealed an invasive disease involving the brain parenchyma, with a cellular composition consistent with an indolent process indicative of possible perinatally acquired infection. one other neonate from our institution with systemic disease due to a nonanthrax bacillus is described, and a review of the english-language literature since 1900 reveals four additional neonates infected with thes ... | 1989 | 2505353 |
| beta-lactamase evaluation of the amoxycillin-clavulanate association. | the capability of potassium clavulanate to protect amoxycillin from destruction by three partially purified beta-lactamases (from e. cloacae, b. cereus 569/h9 and e. coli r-tem) was evaluated. the enzymatic inhibition was determined spectrophotometrically by assessing the ability of either amoxycillin or potassium clavulanate or their combination (at a 7:1 ratio by weight) to prevent the hydrolysis of a chromogenic substrate (nitrocefin) after different pre-incubation times, thus determining the ... | 1989 | 2505995 |
| investigation of neutral endopeptidases (ec 3.4.24.11) and of neutral proteinases (ec 3.4.24.4) using a new sensitive two-stage enzymatic reaction. | a sensitive two-stage enzymatic reaction for mammalian and bacterial metalloendopeptidases has been developed using the substrate 3-carboxypropanoyl-alanyl-alanyl-leucine-4-nitroanilide supplemented with streptomyces griseus amino-peptidase. neutral endopeptidase ec 3.4.24.11 from bovine kidney hydrolyzes the substrate (ph 7.5, 25 degrees c) with a catalytic efficiency (kcat = 1.2 x 10(2) s-1, km = 0.15 mm) of the highest ever reported for the enzyme acting on synthetic chromophoric and fluoroge ... | 1989 | 2507355 |
| apparent phosphate retrieval system in bacillus cereus. | bacillus cereus secretes three different phospholipases c. we studied the effect of pi levels in the growth medium on the production of these exoenzymes. production of both phosphatidylcholine-preferring phospholipase c and sphingomyelinase c was repressed by pi in the growth medium, whereas production of phosphatidylinositol phospholipase c was unaffected. we also found that b. cereus secretes a phosphate-repressed alkaline phosphatase activity. together with a previously reported highly effici ... | 1989 | 2507529 |
| [acute keratitis and contamination of contact lens care systems with bacillus cereus]. | two patients, both contact lens wearers, were treated for acute keratitis in both eyes. routine microbiologic tests done for all four eyes confirmed merely the presence of staphylococcus epidermidis in the conjunctival flora; neither fungi nor any viruses were detected on the patients' corneas. however, the contact lens soaking solutions were contaminated with a large number of microorganisms, including above all--or solely--bacillus cereus. two of the contact lenses used were also colonized by ... | 1989 | 2507821 |
| [incidence of bacillus cereus infections in hungary]. | | 1989 | 2507997 |
| cloning of a gene encoding a major secreted polypeptide of listeria monocytogenes and its potential use as a species-specific probe. | a gene, designated msp, that encodes a major secreted polypeptide with a molecular mass of approximately 60 kilodaltons (kda) was cloned from listeria monocytogenes 10403. dna hybridization analysis indicated that the msp gene was highly conserved among 15 independent l. monocytogenes isolates and that each of 5 isolates tested secreted a 60-kda polypeptide that was immunologically related to the msp gene product. dna sequences related to msp were not detected in any other listeria species or in ... | 1989 | 2508555 |
| [transduction ability of mutants of phage cp51, virulent for bacteria of the bacillus cereus group]. | the virulent phage cp51 used usually to transfer chromosomal and plasmid markers between bacteria of the bacillus cereus group was treated with n-methyl-n'-nitro-n-nitroguanidine. mutants with reduced viability and ts-mutants were isolated. some of the mutants were found to have an increased efficiency of transduction and allow to simplify the process. transfer frequencies of the plasmid pbc16 by the phages cp51-26 and cp51-4-59 were 5 x 10(-4) per plaque-forming unit and 4-5 x 10(-3) per bacter ... | 1989 | 2509285 |
| phosphatidylinositol-specific phospholipase c of bacillus cereus: cloning, sequencing, and relationship to other phospholipases. | the phosphatidylinositol (pi)-specific phospholipase c (plc) of bacillus cereus was cloned into escherichia coli by using monoclonal antibody probes raised against the purified protein. the enzyme is specific for hydrolysis of the membrane lipid pi and pi-glycan-containing membrane anchors, which are important structural components of one class of membrane proteins. the protein expressed in e. coli comigrated with b. cereus pi-plc in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as ... | 1989 | 2509427 |
| treatment of multiple brain abscesses caused by bacillus cereus. | | 1989 | 2512566 |
| dynamic light-scattering studies on the effect of heat and disinfectants on spores of bacillus cereus. | the relative stability of spores of bacillus cereus grown at three different temperatures was examined by using quasi-elastic light scattering (q.l.s.) in conjunction with turbidity and scanning electron microscopy (s.e.m.). cultures grown at 20, 30 and 40 degrees c (bc20, bc30 and bc40 respectively) were compared in terms of (i) their effective hydrodynamic radius, rh, as determined from q.l.s. and (ii) their gross morphology, as determined from s.e.m. the effects of autoclaving at 121.1 degree ... | 1989 | 2512915 |
| trapping the acyl-enzyme intermediate in beta-lactamase i catalysis. | cryoenzymology techniques were used to facilitate trapping an acyl-enzyme intermediate in beta-lactamase i catalysis. the enzyme (from bacillus cereus) was investigated in aqueous methanol cryosolvents over the 25 to -75 degrees c range, and was stable and functional in 70% (v/v) methanol at and below 0 degree c. the value of kcat. decreased linearly with increasing methanol concentration, suggesting that water is a reactant in the rate-determining step. in view of this, the lack of incorporatio ... | 1989 | 2512916 |
| toxin production by bacillus cereus dairy isolates in milk at low temperatures. | a total of 136 strains of bacillus cereus isolated from milk and cream were evaluated for toxin production based on hela s3, vero, and human embryonic lung (hel) cell cytotoxicity in vitro. hel cell monolayers were more susceptible than the other two cell lines. the percentage of isolates exhibiting hel cytotoxicity was similar (43.0 and 48.4%) when the strains were grown in brain heart infusion broth containing 0.1% glucose (bhig) at 7 and 24 h, respectively, at 30 degrees c. in milk, only 21.8 ... | 1989 | 2513777 |
| a comparative study of enzyme variation in bacillus cereus and bacillus thuringiensis. | thirty-two strains of bacillus spp. were examined in a multilocus enzyme study by agarose gel electrophoresis. the organisms were bacillus thuringiensis (21 strains, b. cereus (8), including two of var. mycoides, and b. megaterium (3). strains having similar enzyme variants were grouped into zymovars. a total of 10 of 11 enzyme loci studied were polymorphic and 27 zymovars were distinguished among the 32 strains. the results were subjected to numerical analysis, phenetic affinities and genetic d ... | 1989 | 2515182 |
| [the use of plasmid screening for the differentiation of bacillus anthracis strains from closely related species of soil bacilli]. | b. anthracis virulent and vaccine strains differ from the strains of species closely related to b. anthracis, such as b. cereus and b. thuringiensis, in their plasmid spectrum. the use of their plasmid spectrum. the use of the plasmid analysis of the strain is recommended for laboratory practice as the main differential diagnostic test. | 1989 | 2515698 |
| reactivation of peptidoglycan synthesis in ether-permeabilized escherichia coli after inhibition by beta-lactam antibiotics. | the recovery of peptidoglycan-synthesizing activity after inhibition by beta-lactam antibiotics was investigated in ether-permeabilized cells of escherichia coli b. such cells synthesize sodium dodecyl sulfate-insoluble peptidoglycan when provided with udp-linked precursors and mg2+. the ability of beta-lactam antibiotics to inhibit the synthesis of peptidoglycan was correlated with their affinity for penicillin-binding proteins 1a and 1bs. penicillin-binding protein 1bs is thought to be the maj ... | 1989 | 2515794 |
| bacterial sorption of heavy metals. | four bacteria, bacillus cereus, b. subtilis, escherichia coli, and pseudomonas aeruginosa, were examined for the ability to remove ag+, cd2+, cu2+, and la3+ from solution by batch equilibration methods. cd and cu sorption over the concentration range 0.001 to 1 mm was described by freundlich isotherms. at 1 mm concentrations of both cd2+ and cu2+, p. aeruginosa and b. cereus were the most and least efficient at metal removal, respectively. freundlich k constants indicated that e. coli was most e ... | 1989 | 2515800 |
| cispentacin, a new antifungal antibiotic. i. production, isolation, physico-chemical properties and structure. | a new antibiotic, cispentacin, was isolated from the culture broth of a bacillus cereus strain, l450-b2. the antibiotic is water-soluble and amphoteric; its structure was determined by spectroscopic analysis and chemical synthesis to be (1r,2s)-2-aminocyclopentane-1-carboxylic acid. cispentacin demonstrated only weak in vitro activity against certain fungi but strong protection of mice from lethal infection of candida albicans a9540. | 1989 | 2516082 |
| [the effect of the cultivation conditions on lectin production by representative bacteria in the genus bacillus]. | dynamics of the lectin activity accumulation in the culture liquid was studied through the example of certain representatives of spore-forming aerobic bacteria in the process of their growth. the environmental factors (the temperature, ph value, the presence of definite carbohydrates in the medium) were investigated for their effect on the biosynthesis of extracellular lectins by producers. based on these studies it was possible to establish optimal conditions of cultivation and to obtain the hi ... | 1989 | 2516230 |
| [an alternative to spore formation pathway of viability preservation of bacillus cereus cells in extreme conditions]. | | 1989 | 2516484 |
| survival and growth of bacillus cereus in egyptian bread and its effect on bread staling. | bread was made from dough previously inoculated with spores of three bacillus cereus strains at different inoculation levels. counts of survivors and bread staling were determined in bread during storage at 25 degrees c. the total aerobic bacteria, sporeformer and b. cereus counts in seven different wheat flours were 3.0 x 10(6) to 9.1 x 10(7), 3.2 x 10(5) to 1.5 x 10(6) and 50-100 colony forming units (cfu/g). about one in a thousand bacteria were found to survive baking. on the other hand, no ... | 1989 | 2516878 |
| germicidal effectiveness of dialox, a new stable peroxyacetic acid solution, in the re-use of high-flux dialysers. | in this study we evaluate the effectiveness of a newly available peroxyacetic acid solution (dialox) as a disinfecting agent in the re-use of highly permeable dialysers. the germicidal properties of dialox were tested in an in vitro trial on previously used haemodiafilters (hf80, fresenius) highly contaminated with pseudomonas aeruginosa, mycobacterium smegmatis or sporulated bacillus cereus. complete freedom from bacterial contamination was observed 5 min after the reprocessing treatment on a r ... | 1989 | 2516880 |
| antimicrobial activity of kaurenoic acid derivatives substituted on carbon-15. | the antibacterial and antifungic activities of two kaurenic acids, ent kaurenoic acid and cinnamoylgrandifloric acid isolated from a hexane extract of aerial parts of mikania laevigata, were investigated and compared with the activities of other kaurenic acid derivatives substituted on carbon-15. only acetylgrandifloric acid (ent-kaur-16-en-15 alpha-acetyloxy-19-oic) and its epimer xylopic acid (ent-kaur-16-en-15 beta-acetyloxy-19-oic) displayed significant antibacterial activity at concentratio ... | 1989 | 2517587 |
| [the importance of bacillus cereus in food poisoning]. | in the light of the available literature the present knowledge on the role of bacillus cereus in alimentary intoxications is discussed, particularly the occurrence and role of b. cereus in food pollution, the most frequent alimentary intoxications in the world are reviewed, the biological tests on animals and studies on humans are described, and the metabolites of the organism are presented. particular attention is given to the properties of enterotoxins responsible for diarrhoea and vomiting. | 1989 | 2518144 |
| [determination of the enterotoxic properties of bacillus cereus]. | using indirect biological investigations and various experimental models the enterotoxic properties of 60 strains of b. cereus were determined. the determination of biochemical and enzymatic features was insufficient for indicating the enterotoxic strains and differentiating them from the non-enterotoxic ones. in the dermonecrotic test, vascular permeability test and in cultures on cells reliable criteria for determination of enterotoxicity were obtained as confirmed by the response of the ileum ... | 1989 | 2518145 |
| survival and growth of food poisoning bacteria following inoculation into cottage cheese varieties. | following inoculation into cottage cheese varieties with and without sorbic acid, obtained directly from the manufacturer, strains of enteropathogenic escherichia coli and other e. coli survived but failed to multiply during storage at 7, 10 or 25 degrees c. in the absence of sorbic acid spoilage due to pseudomonas fluorescens occurred after storage for 5-13 days at 7 or 10 degrees c and 1-2 days at 25 degrees c. salmonella enteritidis, s. hadar, s. saint-paul, s. typhimurium and s. virchow surv ... | 1989 | 2518228 |
| experimental observations on the bacteriological controls of antibiotics--ii. inactivation of the antimicrobial activity of membranes employed for the filtration of antibiotics. | | 1989 | 2518775 |
| bioassay of teicoplanin in serum containing rifampin or a beta-lactam antibiotic. | the purpose of this study was to develop bioassays for the measurement of teicoplanin in serum containing rifampin or a beta-lactam antibiotic. use of rifampin-resistant bacillus subtilis as indicator organism or pretreatment of the serum sample with bacillus cereus penicillinase type i (nafcillin, ticarcillin, mezlocillin) or type ii (cefazolin, cefuroxime, ceftazidime, ceftriaxone) effectively eliminated assay interference. validation bioassays performed on two separate days utilizing triplica ... | 1989 | 2533051 |
| molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase c) of clostridium perfringens. | a fragment of dna containing the gene coding for the phospholipase c (alpha-toxin) of clostridium perfringens was cloned into escherichia coli. the cloned dna appeared to code only for the alpha-toxin and contained both the coding region and its associated gene promoter. the nucleotide sequence of the cloned dna was determined, and an open reading frame was identified which encoded a protein with a molecular weight of 42,528. by comparison of the gene sequence with the n-terminal amino acid sequ ... | 1989 | 2536355 |
| involvement of tn4430 in transfer of bacillus anthracis plasmids mediated by bacillus thuringiensis plasmid pxo12. | the self-transmissible plasmid pxo12 (112.5 kilobases [kb]), originally isolated from strain 4042a of bacillus thuringiensis subsp. thuringiensis, codes for production of the insecticidal crystal protein (cry+). the mechanism of pxo12-mediated plasmid transfer was investigated by monitoring the cotransfer of the tetracycline resistance plasmid pbc16 (4.2 kb) and the bacillus anthracis toxin and capsule plasmids, pxo1 (168 kb) and pxo2 (85.6 kb), respectively. in matings of b. anthracis donors wi ... | 1989 | 2536653 |
| a bacillus cereus cytolytic determinant, cereolysin ab, which comprises the phospholipase c and sphingomyelinase genes: nucleotide sequence and genetic linkage. | a cloned cytolytic determinant from the genome of bacillus cereus gp-4 has been characterized at the molecular level. nucleotide sequence determination revealed the presence of two open reading frames. both open reading frames were found by deletion and complementation analysis to be necessary for expression of the hemolytic phenotype by bacillus subtilis and escherichia coli hosts. the 5' open reading frame was found to be nearly identical to a recently reported phospholipase c gene derived fro ... | 1989 | 2536680 |
| production, purification and spectral properties of metal-dependent beta-lactamases of bacillus cereus. | new methods for the production of consistently high levels of metal-dependent beta-lactamases (beta-lactamhydrolase, ec 3.5.2.6) from strains 569/h/9 and 5/b/6 of bacillus cereus are described which have significant advantages over those reported previously. for example, these techniques do not require a fermentor with ph-stat capabilities. we also describe rapid very-high-yield purification schemes for the metal-dependent beta-lactamases from these strains, employing high-performance ultrafiltr ... | 1989 | 2539866 |
| neutrophil oxidative metabolism after exposure to bacterial phospholipase c. | the effects of phospholipase c (plc) from clostridium perfringens and bacillus cereus on bovine neutrophil oxidative metabolism were studied by measuring superoxide production and oxygen consumption in response to plc alone or in combination with other stimuli. plc from both species elicited superoxide production and enhanced the response to stimulation by naf when the two stimuli were given simultaneously. however, oxygen consumption in response to latex beads was markedly inhibited by pretreat ... | 1989 | 2544653 |
| phospholipase c and haemolytic activities of clostridium perfringens alpha-toxin cloned in escherichia coli: sequence and homology with a bacillus cereus phospholipase c. | the clostridium perfringens alpha-toxin (phospholipase c) gene (cpa) has been cloned and expressed in escherichia coli. the biological activities of the cloned gene product have been analysed and the complete nucleotide sequence of the cpa gene has been determined. the cloned cpa gene product, which is exported to the periplasm in e. coli, possesses both phospholipase c and haemolytic activities. haemolysis is not apparent when cell extracts are incubated with isotonic suspensions of sheep eryth ... | 1989 | 2546005 |
| efficient transformation of bacillus thuringiensis and b. cereus via electroporation: transformation of acrystalliferous strains with a cloned delta-endotoxin gene. | electroporation was used as a method to transform intact cells of bacillus thuringiensis and b. cereus. with our optimized method a range of plasmid vectors could be transformed into strains of b. thuringiensis at frequencies of up to 10(7) transformants/micrograms dna. this high frequency allows cloning experiments to be done directly in b. thuringiensis. a bifunctional vector capable of replicating in escherichia coli and in bacillus spp. was constructed. the kurhd1 protoxin gene was cloned in ... | 1989 | 2550762 |
| functional characteristics of phosphatidylinositol-specific phospholipases c from bacillus cereus and bacillus thuringiensis. | phosphatidylinositol-specific phospholipase c was purified from the culture medium of b. thuringiensis to high specific activity using a procedure we recently described for purification of pi-plc from b. cereus (volwerk et al. (1989) j. cell. biochem. 39, 315-325). the purified enzymes from b. thuringiensis and b. cereus have similar specific activities towards hydrolysis of the membrane lipid phosphatidylinositol, and also towards hydrolysis of the glycosyl-phosphatidylinositol-containing membr ... | 1989 | 2558947 |
| gene cloning shows the alpha-toxin of clostridium perfringens to contain both sphingomyelinase and lecithinase activities. | the plc gene encoding the alpha-toxin (phospholipase c), an important virulence factor of clostridium perfringens, has been cloned, sequenced and expressed in escherichia coli. transcriptional analysis of mrnas produced in vivo by c. perfringens and e. coli, and in vitro using purified rna polymerase from c. perfringens revealed that plc is transcribed constitutively from a single promoter situated about 100 nucleotides from the coding sequence. a t7 expression system was used to overproduce alp ... | 1989 | 2560137 |
| preincubation time and the use of oxygen indicators in determining the microbiological quality of aseptically packed pea and tomato soup. | in order to get accurate information about the preincubation time needed for viscous aseptic products, the growth characteristics of three food poisoning organisms staphylococcus aureus, clostridium perfringens and bacillus cereus in pea soup, and one spoilage organism, lactobacillus plantarum, in tomato soup, were followed during a preincubation period at 30 degrees c for 14 days. the sterile food packs were sealed into polyamidepolyethylene laminate bags containing different headspace gas conc ... | 1989 | 2561900 |
| antibacterial effect of the glucose oxidase-glucose system on food-poisoning organisms. | the antibacterial effect of the glucose oxidase-glucose system was studied on food-poisoning organisms including staphylococcus aureus, salmonella infantis, clostridium perfringens, bacillus cereus, campylobacter jejuni, listeria monocytogenes and yersinia enterocolitica using automated turbidometry. the bacteria were grown in sterile-filtered meat medium which was either raw or heat-denaturated. the results showed a clear growth inhibition with combinations of 0.5-1.0 mg/ml glucose and 0.5-1.0 ... | 1989 | 2561954 |
| primary cutaneous bacillus cereus infection in neutropenic children. | a review of culture reports for a five-year period at st jude children's research hospital yielded 10 cases of primary cutaneous bacillus cereus infection in neutropenic patients treated for cancer or aplastic anaemia. vesicles or pustules were seen only on the limbs. the infections, all of which arose in the spring or summer, responded to antibiotics. in neutropenic patients b cereus should thus be regarded as a possible cause of isolated vesicles. | 1989 | 2564122 |
| nucleotide sequence of the glutamine synthetase gene (glna) and its upstream region from bacillus cereus. | we have determined the complete nucleotide sequence of a 2.4 kb chromosomal ecot22i-nspv fragment, containing the bacillus cereus glna gene (structural gene of glutamine synthetase). the deduced amino acid sequence indicates that the glutamine synthetase subunit consists of 444 amino acid residues (50,063 da). comparisons are made with reported amino acid sequences of glutamine synthetases from other bacteria. upstrem of glna we found an open reading frame of 129 codons (orf129) preceded by the ... | 1989 | 2572584 |
| heterogeneity of 5s rna in fungal ribosomes. | neurospora crassa has at least seven types of 5s rna genes (alpha, beta, gamma, epsilon, delta, zeta, and eta) with different coding regions. a high resolution gel electrophoresis system was developed to separate minor 5s rna's from the major 5s rna (alpha). a study of several neurospora crassa strains, four other species in the genus neurospora, members of two closely related genera, and three distantly related genera demonstrated that 5s rna heterogeneity is common among fungi. in addition, di ... | 1985 | 2579431 |
| [dry nutrient medium for determining the lecithinase activity of bacillus cereus]. | | 1985 | 2581021 |
| specificity of bacillus thuringiensis for lepidopteran larvae: factors involved in vivo and in the structure of a purified protoxin. | the relative ld50 values in two test lepidoptera of bacillus thuringiensis subspecies kurstaki hd1, which contains three cryla protoxin genes, was the same as a plasmid-cured derivative or a bacillus cereus transcipient containing only one of the three genes. differential rates of transcription of these genes in the original strain could account, at least partly, for this result. strains containing only the single protoxin gene (cryla(b] produced inclusions when grown at 25 degrees c but not 32 ... | 1989 | 2615654 |
| isolation and fundamental properties of a phospholipase a2 inhibitor from the blood plasma of trimeresurus flavoviridis. | phospholipase a2 inhibitor was purified from the blood plasma of habu, trimeresurus flavoviridis, by sephadex g-200 gel filtration, deae-cellulose chromatography, and blue-sepharose cl-6b column chromatography. the purified inhibitor was shown to be a glycoprotein with a molecular weight of about 100k. it was found to consist of four subunits whose molecular weights were around 20-24k. in order to examine the inhibition mechanism of the inhibitor, the interaction of the inhibitor with a phosphol ... | 1989 | 2628432 |
| the nucleotide sequence of the lipo-penicillinase gene of alkalophilic bacillus sp. strain 170. | the lipo-penicillinase (lipen) gene from an alkalophilic bacillus sp. strain 170 was cloned in escherichia coli using the vector phsg399. a plasmid, pfap121, was isolated from an ampicillin resistant transformant and the cloned lipen gene was found to be in a 2.2 kb dna fragment. the nucleotide sequence of a 1.9 kb segment encoding the lipen was determined. this segment showed an open reading frame which would encode a polypeptide of 310 amino acids. the amino acid sequence of this lipen gene pr ... | 1989 | 2655551 |
| screening of isoquinoline alkaloids and their derivatives for antibacterial and antifungal activities. | a screening test of antimicrobial activities for some of the isoquinoline alkaloids and their first and second hofmann elimination products was conducted in this study. results showed that (+)-actinodaphnine (1), (+)-n-me-actinodaphnine (2), (+)-anonaine (17), (-)-xylopine (19) and (-)-n-me-xylopine mei (20), had the strongest inhibitory activities against three g(+) bacteria (bacillus cereus, micrococcus sp. and staphylococcus aureus) mic greater than or equal to 50 micrograms/ml). whereas anhy ... | 1989 | 2733073 |
| mortality in adult tsetse, glossina morsitans morsitans, caused by entomopathogenic bacteria. | mortality in adult tsetse, glossina morsitans morsitans, caused by pseudomonas aeruginosa, serratia marcescens, bacillus sphaericus, bacillus cereus, bacillus thuringiensis h-14, b. thuringiensis 1, b. thuringiensis 5, b. thuringiensis var. insraelensis, and providentia rettgeri was determined. when bacteria were smeared on rabbit skin and tsetse allowed to feed only once on the contaminated area, mortality 8 days postingestion was significantly higher (p less than 0.01) in tsetse fed on p. aeru ... | 1989 | 2738417 |
| effect of exposure of pieris brassicae larvae to 2,4,5-trichlorophenoxyacetic acid on the natural antibacterial activity of serum. | larvae of the cabbage white butterfly, pieris brassicae, were reared on a semisynthetic diet with or without 20 ppm of the herbicide 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) and using three assays the sera were subsequently tested for natural antibacterial activity against bacillus cereus, escherichia coli k12, and micrococcus luteus. these assays showed that exposure of larvae to 2,4,5-t lowered the antibacterial activity of the serum against e. coli and m. luteus compared with control anima ... | 1989 | 2738419 |
| demonstration of functional acetylcholinesterase on the soma of individual neurones of aplysia by in vivo microspectrophotometry. | the presence of functional acetylcholinesterase is demonstrated in vivo on somatic membranes of single ganglionic neurones of aplysia using concurrently microspectrophotometry and electrophysiology. the similarity of the effects of an irreversible blocker of acetylcholinesterase and of phospholipase c from bacillus cereus suggests that acetylcholinesterase is anchored in the membrane via phosphatidylinositol. | 1989 | 2765894 |
| isolation of a staphylococcus aureus beta-lactamase-dicloxacillin complex and kinetic studies on the reactivation of the enzyme. | exposure of the beta-lactamase from staphylococcus aureus to the slowly reacting substrates cloxacillin or dicloxacillin results in time-dependent inactivation of the enzyme. methods for the rapid separation of a beta-lactamase-dicloxacillin complex from excess inhibitor, using centrifuged columns of sephadex g-25 or deae-sephadex g-25, are described. the enzyme-dicloxacillin complex releases active enzyme, with specific activity identical to that of untreated enzyme, after storage at ph 7.5 at ... | 1989 | 2783544 |
| [l-forms of aerobic spore-forming bacteria isolated from urologic patients]. | new data are presented on the ability of different aerobic spore-forming bacteria isolated from the organism of urological patients to produce l-forms of these microorganisms in the presence of penicillin and ampicillin. bacillus cereus is shown to be the most resistant to these antibiotics. | 1989 | 2796790 |
| [structure and biological features of the temperate phage of bacillus thuringiensis var. galleriae 69/9, sensitive to chloroform]. | a new temperate bacteriophage designated px1 has been isolated from the culture of bacillus thuringiensis var. galleriae 69/6 producing enthobacterin. the bacteriophage belongs to morphological group b1 in accordance with the classification by d. reanney and h. ackerman. the bacteriophage head has an isometric multifaceted form with 40 nm diameter. the length of its noncontractile transversely lined tail is 130 nm. high sensitivity to chloroform is peculiar of the phage. the lytical specter of t ... | 1989 | 2811909 |
| transfer of chromosomal genes and plasmids in bacillus thuringiensis. | a low frequency of chromosomal gene transfer from bacillus thuringiensis to bacillus cereus was detected by cell mating, with a tryptophan marker being the most frequently transferred gene among four that were tested. the process was resistant to dnase and was not mediated by cell filtrates. among several b. thuringiensis subspecies tested, transfer was best with a derivative of b. thuringiensis subsp. kurstaki hd1, which lost several plasmids. all of the b. cereus recombinants contained at leas ... | 1987 | 2821899 |
| renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c. | renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ... | 1987 | 2822007 |
| [homologous and heterologous transcription of the cry+-plasmid in bacillus thuringiensis]. | the possibility of homologous and heterologous transception of cry+ plasmids in bacillus thuringiensis is demonstrated. cry+ plasmids from crystal bearing strain of bacillus thuringiensis were transferred into acrystalline strain belonging to h5 serotype by mutual incubation. the donor strain was previously marked by the transmissive plasmid pam beta 1 coding for erythromycin and lincomycin resistance. the transcipients having acquired the ability to synthesize delta-endotoxin were referred to h ... | 1987 | 2828933 |