| flow cytometry of human spermatozoa. | methods are given for the preparation and staining of human spermatozoa for flow cytometric dna measurements. using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes an effective decondensation of the sperm chromatin and a dna-proportional uptake of fluorochromes is achieved. thus reliable and precise measurements of the relative dna content of human spermatozoa are possible and the two subpopulations of haploid spermatozoa can be distinguished according ... | 1979 | 90040 |
| [topo-optical reactions on the membrane of the human red cell ghost (author's transl)]. | previous studies have proved that the thiazin dyes toluidine blue, azure a, azure b, 1.9-dimethyl methylene blue and the quinolin dyes n,n'-diethylpseudoisocyanine chloride, n,n'-6,6'-dichlorpseudoisocyanine chloride are suitable for topo-optical reaction on the membrane of the red blood cells. in the present study the applicability of the thiazin and quinolin dyes on the membrane of the human red cell ghost was examined. optical analysis revealed that the thiazin dyes are bound in radial positi ... | 1979 | 90042 |
| immunochemical comparison of myosin light chains from chicken fast white, slow red, and cardiac muscle. | antibodies were formed against the myosin light chains isolated from chicken fast skeletal, slow skeletal, and cardiac muscle and the antigenicities of the light chains were compared by double immunodiffusion and immunoelectrophoresis. it was shown that fast light chains are immunologically different from light chains of slow and cardiac myosin, while the slow and cardiac muscle light chains have similar immunological characteristics; that is, the light chains of apparent molecular weight about ... | 1979 | 90044 |
| alpha-fetoprotein gene expression. control of alpha-fetoprotein mrna levels in cultured rat hepatoma cells. | the expression of alpha-fetoprotein (afp) was infestigated in a cloned cell culture derived from morris hepatoma 7777, which shows a density-dependent variation in the afp synthesis rate. the rate of secretion of afp was found to be governed by the level of cytoplasmic mrnaafp. saturation hybridization of pulse-labeled rna to excess cloned cdnaafp was used to illustrate quantitatively how mrnaafp is regulated in these tumor cells. it was found that the mrnaafp level is primarily determined by it ... | 1979 | 90046 |
| anionic sites in the glomerular basement membrane. in vivo and in vitro localization to the laminae rarae by cationic probes. | cationized ferritin (cf) of narrow pi range (7.3-7.5) and the basic dye ruthenium red (rr) have been used as cationic probes to partially characterize anionic sites previously demonstrated in the glomerular basement membrane (gbm). when cf was given i.v. to normal rats and the left kidney was fixed by perfusion 15 min thereafter, clusters of cf molecules were found throughout the lamina rara interna (lri), lamina rara externa (lre), and mesangial matrix distributed at regular (approximately 60 n ... | 1979 | 90048 |
| slowly migrating axonal polypeptides. inequalities in their rate and amount of transport between two branches of bifurcating axons. | polypeptides in the dorsal root ganglion (l5) of the adult rat were radioactively labeled, and components slowly migrating in the sciatic nerve (peripheral axons) and dorsal root (central axons) were analyzed, using sds-polyacrylamide slab gel electrophoresis and fluorography. in particular, the transport rates and amounts of six major polypeptides, i.e., the triplet (reference 15; with mol wts of 200,000, 160,000, and 68,000 daltons), alpha- and beta-tubulins and actin were compared between the ... | 1979 | 90050 |
| actin in spindles of haemanthus katherinae endosperm. i. general results using various glycerination methods. | we have studied actin-containing filaments in spindles in haemanthus endosperm cells glycerinated by various methods; the actin-containing filaments were identified by their reaction with rabbit skeletal muscle heavy meromyosin (hmm) to form 'decorated' filaments. actin-containing filaments in the spindle were seen in amongst microtubules in bundles (both non-kinetochore microtubule bundles and kinetochore microtuble bundles) and were also seen not associated with microtubules. there were very f ... | 1979 | 90052 |
| comparative serology of two clinical isolates of bacteroides fragilis and bacteroides thetaiotaomicron. | antiserum prepared in rabbits against bacteroides fragilis showed numerous bands when reacted with b. fragilis antigen in ouchterlony plates. this antiserum also reacted with bacteroides thetaiotaomicron and showed one band of apparent identity with b. fragilis. the band of identity common for both organisms was lost if the antigen was heated at 80 degrees c for 30 min. antisera prepared against b. thetaiotaomicron did not react with b. fragilis. | 1979 | 90054 |
| serum concentration of human alpha 2 hs glycoprotein during the inflammatory process: evidence that alpha 2 hs glycoprotein is a negative acute-phase reactant. | a nonspecific opsonin function has been ascribed to human alpha 2 hs glycoprotein. its serum level has been shown to be decreased in trauma patients. recent studies from this laboratory revealed a heterogeneity among the products obtained in the course of the preparation of the protein. to date, no definitive agreement existed with regard to a molecular homogeneous entity of alpha 2 hs glycoprotein (ba-alpha 2 glycoproteins). the purpose of the current work was to study the variations in serum l ... | 1979 | 90057 |
| combined functional and immunochemical analysis of normal and abnormal human factor x. | human factor x was isolated from cohn fraction iii and characterized by polyacrylamide gel electrophoresis, amino acid composition, and isoelectric focusing. two molecular forms with biological activity were observed at isoelectric points of 4.8 and 5.0. antisera generated to factor x was monospecific and used to establish an equilibrium competitive inhibition radioimmunoassay. this assay was specific for human factor x and did not cross-react with human prothrombin or bovine factor x within the ... | 1979 | 90058 |
| the histochemical distribution of protein bound sulfhydryl groups in human epidermis by the new staining method. | recently, we synthesized a new fluorescent thiol reagent, n-(7-dimethylamino-4-methylcoumarinyl)-maleimide (dacm) which is nonfluorescent by itself but will react readily with -sh groups to form highly fluorescent addition products. by the use of this reagent, we studied the localization and concentration of -sh groups and s--s linkages in the human epidermis. the distribution of -sh groups in living layers was abundant in cytoplasm but not in nuclei. the fluorescence was concentrated on the cel ... | 1979 | 90070 |
| immunoferritin location of carbamoyl phosphate synthetase in rat liver. | experiments were carried out to locate carbamoyl phosphate synthetase (cps) in rat liver by direct immunoferritin labeling. by using epon sections treated with sodium methoxide, homogenates or mitochondrial and mitoplast fractions, carbamoyl phosphate synthetase was found homogeneously distributed in the mitochondrial matrix. immunoferritin was detected with high resolution which permits the identification of individual molecules. measurements were made of the number of ferritin particles per sq ... | 1979 | 90072 |
| enzymatic tracers in the study of vascular permeability. | elucidation of the ultrastructural basis of vascular permeability was aided by the development of cytochemical techniques for visualizing the distribution, within the vessel wall, of intravenously injected peroxidatic enzymes of varying molecular size. tracer enzymes available range from 10 a (hemeoctapeptide) to 52 a (catalase) effective molecular radius. the use of enzymatic probe molecules assumes a thorough characterization of: (a) the molecular charge (isoelectric point of the native enzyme ... | 1979 | 90073 |
| alloantigen bound to agarose beads and syngeneic carrier cells are capable of stimulating mouse cytolytic t lymphocytes in vitro. | bead-bound antigen was prepared by coupling alloantigen covalently to agarose beads. alloantigen-bearing syngeneic carrier cells were prepared by dilution of detergent solubilized alloantigen in the presence of syngeneic spleen cells. both types of antigen were compared to spleen cells and reconstituted membrane fragments for the ability to stimulate cytolytic thymus-dependent lymphocytes in vitro. all these types of antigen could stimulate immune but not nonimmune spleen cells to form cytolytic ... | 1979 | 90085 |
| a hapten-specific ir gene. | when mice of different strains were immunized with a conjugate of 2-phenyloxazolone (phox) and chicken serum albumin (csa), the antibody response was controlled by an ir gene (ir-phox). h-2 alleles d and f were associated with a high response, k and a with an intermediate response, and allele b with a low response. the effect of the ir gene was clear-cut in anti-carrier antibodies of the primary and the secondary response when the concentrations of anti-carrier antibodies varied between 1 and 35 ... | 1979 | 90089 |
| modulation of the host response in human schistosomiasis. i. adherent suppressor cells that inhibit lymphocyte proliferative responses to parasite antigens. | | 1979 | 90093 |
| involvement of host fc receptors in antibody-mediated cutaneous basophil hypersensitivity reactions. | cutaneous basophil hypersensitivity (cbh) reactions are heterogeneous delayed time course basophil-rich responses that can be mediated by either t cells, b cells, or serum antibodies. the current study examined the mechanism by which antibodies mediate cbh in guinea pigs. fc competition experiments were constructed by passively transferring mixtures of anti-klh serum and normal heterologous gamma-globulins. it was found that rabbit igg and its isolated and purified fc fragment [but not the (fab' ... | 1979 | 90094 |
| effects of arachidonic acid and its metabolites on antigen-induced histamine release from human basophils in vitro. | | 1979 | 90097 |
| characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte fc receptors. | to investigate the antigenic relationship between the macrophage and lymphocyte fc receptors (fcr), a monoclonal antibody capable of blocking mouse macrophage fc receptors was selected. hybrids were formed by fusing the p3u1 mouse myeloma and spleen cells from a rat immunized with the mouse macrophage-like cell lines j774 and p388d1. the fab fragment of the monoclonal igg secreted by clone 2.4g2, inhibited the trypsin-resistant fc receptor ii (fcrii), which is specific for immune aggregates of m ... | 1979 | 90108 |
| the strategy of infection as a criterion for phylogenetic relationships of non-coli phages morphologically similar to phage t7. | five phages which are morphologically similar to coliphage t7 but attack other host bacteria have been compared to t7 and to its relative, t3, by the following criteria: (a) cross-reactivity with antisera against t7 and t3, (b) dna base sequence homologies, as determined by the c0t technique, (c) synthesis of two phage-coded enzymes: rna polymerase and samase, (d) patterns of phage-directed protein synthesis, as determined by sds-polyacrylamide gel electrophoresis of phage coat subunits. as judg ... | 1979 | 90110 |
| immunochemical and oligonucleotide fingerprint analyses of venezuelan equine encephalomyelitis complex viruses. | rna oligonucleotide fingerprint analyses indicate that the genome rna obtained from trinidad donkey (trd) venezuelan equine encephalomyelitis (vee) virus serotype i a, its vaccine strain derivative tc-83, and the vee i b virus isolate ptf-39, have almost identical patterns of characteristic ribonuclease t1 resistant oligonucleotides. the tc-83 strain and the i b isolate can, on the basis of these analyses, be considered as variants of the trd virus and categorized as i ab serotypes. comparisons ... | 1979 | 90115 |
| a radioimmunoassay for virus antibody using binding of 125i-labelled protein a. | an assay for virus antibodies using protein a from staphylococcus aureus is described. type b and type c rna tumour viruses adsorbed on to polystryrene microtitre plate wells were incubated with antiserum and then with 125i-labelled protein a (i-pa) and bound radioactivity was determined. technical details such as labelling, antigen concentration, storage of i-pa are reported. the specificity of the reaction was investigated in detail by competition experiments with purified unbound homologous v ... | 1979 | 90116 |
| antigenic relationships between polypeptides derived from plantar and hand wart viruses. | guinea pigs immunized with polypeptides derived from plantar and hand wart viruses developed both humoral and cell mediated immunity. the specificity of the antiserum was determined by indirect immunofluorescence (if) tests and cellular immunity by intradermal tests. while whole plantar and hand wart virus particles (pv and hv) appeared to be immunologically analogous, they had different polypeptide patterns as shown by analysis on acrylamide slab gels. in particular, the p2 polypeptide (major v ... | 1979 | 90120 |
| the effect of cop 1, a synthetic polypeptide, on chronic relapsing experimental allergic encephalomyelitis in guinea pigs. | cop 1, a synthetic polypeptide, was evaluated for its effect on a chronic relapsing form of experimental allergic encephalomyelitis (eae). pretreatment of juvenile strain 13 guinea pigs with cop 1 in incomplete freund's adjuvant (ifa) which were subsequently challenged with guinea pig spinal cord in complete freund's adjuvant (cfa) had a marked effect in delaying or preventing the appearance of clinical signs of eae. administration of cop 1 on appearance of clinical signs of eae prevented progre ... | 1979 | 90129 |
| rapid detection of malaria and other bloodstream parasites by fluorescence microscopy with 4'6 diamidino-2-phenylindole (dapi). | dapi is a fluorescent dye which appears to complex specifically with dna. we have used this probe to detect and identify malarial infections by fluorescence microscopy. experiments were conducted using plasmodium berghei yoeli--infected mouse blood, p. lophurae--infected duck blood, and p. vivax--infected human blood. infected avian blood was used to detect parasites within nucleated erythrocytes. control blood smears from uninfected hosts revealed fluorescence only in the leukocytes of mammalia ... | 1979 | 90141 |
| comparison of histochemical studies of intestinal atresia in the human newborn and fetal lamb. | | 1979 | 90142 |
| comparative pharmacokinetics of coumarin anticoaglulants xli: effect of phenobarbital on systemic availability of orally administered dicumarol in rats. | | 1979 | 90143 |
| adsorption, penetration, and uncoating of murine leukemia virus studied by using its reverse transcriptase. | a procedure using the virus-associated reverse transcriptase was developed for following the kinetics of adsorption, penetration, and uncoating of murine leukemia virus. viral adsorption to cell membrane was determined by assaying this enzyme activity in isolated debris of mechanically disrupted cells after infection with murine leukemia virus in the presence of actinomycin d. at 37 degrees c, viral adsorption proceeded at a high initial rate, but after 5 min of incubation with the virus, it gra ... | 1979 | 90159 |
| biochemical characterization of the type c retrovirus associated with lymphoproliferative disease of turkeys. | turkeys inoculated with spleen extracts from lymphoproliferative disease (lpd)-affected birds developed viremia, followed by typical lpd lesions. electron microscopy and biochemical characterization established that the virus present in the blood of infected turkeys is a type c retrovirus. the viral particles possess a buoyant density of 1.17 g/ml in sucrose gradients; they contain high-molecular-weight rna and an rna-instructed dna polymerase with efficient exogenous and endogenous activity. th ... | 1979 | 90160 |
| identification of the primate papovavirus hd as the stump-tailed macaque virus. | the recently isolated primate papovavirus hd is shown to be indistinguishable from the stump-tailed macaque virus by immunofluorescent reactivity, by restriction endonuclease analysis, and by nucleic acid hybridization assay. | 1979 | 90163 |
| optimal conditions for synthesis of long complementary dna product with moloney murine leukemia virus. | studies are described on the interrelationships between divalent metals, dntp's and ppi in determining the properties of complementary dna (cdna) product from the in vitro reverse transcriptase reaction with detergent-treated moloney murine leukemia virus. in spite of the several-fold greater amount of cdna product with mn2+ than with mg2+, net yield of high-molecular-weight cdna was much greater with mg2+ thant with mn2+. this held true, as well, for the reactions containing excess dntp or dntp ... | 1979 | 90169 |
| radioimmunoassays for the 36,000-dalton glycoprotein of murine mammary tumor viruses demonstrate type, group, and interspecies determinants. | mouse mammary tumor viruses (mmtvs) contain distinct membrane glycoproteins of 52,000 daltons (gp52) and 36,000 daltons (gp36). we report here the development of new radioimmunoassays for gp36, using gp36 purified by hydrophobic chromatography and gel filtration. these assays demonstrate that gp36 has both type-specific and group-specific antigenic determinants. the virus-coded nature of these determinants was shown by utilizing different mmtvs grown in the same feline cell line. interspecies d ... | 1979 | 90170 |
| [micromethod of carrying out the passive hemagglutination reaction for detecting antibodies to influenza a2 virus in tears and aqueous humor]. | | 1979 | 90190 |
| [improving the effectiveness of virological studies in influenza]. | | 1979 | 90208 |
| t and b lymphocytes in patients with acute anterior uveitis and ankylosing spondylitis, and in their household contacts. | during episodes of acute anterior uveitis, patients had a t-lymphopenia and a temporary increase in b-lymphocytes. the t-lymphopenia was not present in patients investigated early in their first attacks, and it persisted after the patients recovered clinically. in household contacts of patients with uveitis, there was a temporary t-lymphopenia. a similar degree of t-lymphopenia was present in patients with ankylosing spondylitis who had not had uveitis, but not in their household contacts. in pa ... | 1979 | 90213 |
| gamma-aminobutyric acid in human cerebrospinal fluid: normal values. | | 1979 | 90262 |
| surface structure of mammalian articular cartilage viewed at low temperature. | | 1979 | 90268 |
| detection of herpes-simplex viral genome in brain tissue. | herpes-simplex virus type (hsv-1) nucleic-acid sequences were detected by in-situ hybridisation in thin sections of brains from mice which had been inoculated 24 weeks previously with hsv-1. these mice were not ill, and infectious virus could not be recovered from them. hsv-1 sequences were also present in brain smears from 3 of 4 elderly patients who had died with chronic psychiatric illness and neuropathological changes but not in smears from 2 patients who had had acute psychotic episodes and ... | 1979 | 90272 |
| are there antigenic variants of hepatitis a virus? | | 1979 | 90289 |
| rubella screening by hemolysis-in-gel test. | | 1979 | 90313 |
| rubella screening by hemolysis-in-gel-test. | | 1979 | 90314 |
| [species and interspecies antigenic specificity of house dust mites (genus dermatophagoides)]. | | 1979 | 90327 |
| the relation between chemically induced sister-chromatid exchanges and chromatid breakage. | studies of classical chromosome aberrations and sister-chromatid exchanges (sces) suggest independent mechanisms for the two events despite some common features. examination of chromosome breakage caused by x-rays, visible light, and viruses has shown that few chromatid breaks are accompanied by sces at the sites of breaks. no similar observations were available for chemically induced breaks, but it has been reported that rat chromosomes exposed to dimethylbenzanthracene (dmba) contained a prepo ... | 1979 | 90339 |
| the elephant man. | | 1979 | 90342 |
| [histological and ultrastructural observations of rabbit aorta intraparietally injured by mechanical trauma (author's transl)]. | | 1978 | 90357 |
| corticosteroid and antihistamine modification of bleomycin-induced fever. | | 1979 | 90370 |
| the weight of the human prostate. | the weight of the prostate proper was determined in an autopsy series comprising 205 cases. the dissection technique of tisell & salander made it possible to delimit the prostate proper from non-prostatic tissue. the mean weight of the "normal" prostate in adult men was about 11 grams (range 7--16). this weight as well as the water content did no change with age. individuals who died after protracted disease had lower prostatic weight. the presence of periurethral adenomas was associated with a ... | 1979 | 90380 |
| immunological approaches to the structure of fibrinogen. | | 1979 | 90395 |
| antigenic drift in type a influenza virus: sequence differences in the hemagglutinin of hong kong (h3n2) variants selected with monoclonal hybridoma antibodies. | | 1979 | 90425 |
| [pharmacological properties and antitumor activity of a preparation diiodo-benzo-tepa in experiments]. | experimental data on the pharmacokinetics, toxicity, antitumor activity of the antitumor drug diiodo-benzo-tepa are reported. | 1979 | 90429 |
| [detection of humoral antibodies to species-specific and group ornithosis antigens in the dynamics of the infection]. | the time course of antibody production to group- and species-specific antigens of ornithosis agent was studied by complement fixation (cft) and hemagglutination-inhibition (hi) tests. in rabbits after a single intravenous inoculation of the ornithosis agent, antibody to the homologous species-specific antigen appeared in the peripheral blood 3--5 days after inoculation and reached maximum levels during the 1-st week, and to the group-specific antigen could be detected 3--5 days after inoculation ... | 1979 | 90431 |
| analysis of antigens in mycobacterium paratubercuolsis. | | 1979 | 90456 |
| current status of prostate androgen receptors. | over the past eight years, many investigators have attempted to measure androgen receptors in human prostate cytosol and nuclear extracts. it is clear that the concentration of unoccupied cytosol receptors is quite low though measurable if appropriate techniques such as agar gel electrophoresis, sucrose density gradient centrifugation (sdgc) or protamine sulfate precipitation are employed. attempts to correlate these values in untreated patients with their subsequent response to therapy have not ... | 1979 | 90476 |
| [isolation of the membranes of actinomyces sp. 26-115, a producer of actinomycin c]. | a summation fraction of the membranes of actinomyces sp. 26-115 was obtained as a result of lysis of its protoplasts in a hypotonic medium. the qualitative content of protein, lipids, phospholipids, nucleic acids, glucosamine and muramic acid was determined in the membranes at various stages of the organism development. phosphatidylcholine is the main component of phospholipids in this organism. intracellular actinomycin was found inside the protoplasts. electrophoregrams of the microprotoplasts ... | 1979 | 90478 |
| plasma level of the prodrug midodrine and its active metabolite in comparison with the alpha-mimetic action in dogs. | midodrine, i.v. or orally administered, causes a prolonged elevation of blood pressure and a reduction in heart rate. these cardiovascular changes are not correlated to the plasma levels of the intact drug. on administration of either midodrine or its metabolite, st-1059, formed by cleavage of the glycine residue, the elevation of blood pressure and the reduction in heart rate were significantly correlated to the plasma level of st-1059. the results are in agreement with the assumption that the ... | 1979 | 90492 |
| intracellular potential changes of corti's organ with anoxia. | intracellular measurements of the resting cell membrane potentials of guinea pig corti's organ were made in order to determine the sensitivity of this cell potential to anoxic hypoxia (a lowered oxygen state due to lack of respiratory oxygen) and to establish differences according to cell types or morphologic regions of the sensory epithelium. the negative cell potentials measured from successful electrode penetrations were found to be relatively more stable and resistant to change during a 120- ... | 1979 | 90497 |
| relationship of centrifugal fibres to 'supporting' cells. | we have described cholinergic fibres which cross the tunnel to three levels of the cells on the opposite side--from the level of the efferent network to the base of a plaque at the level of the nucleus of the first deiters cell. these fibres are dependent upon the ocb being intact. the same is true for those fibres seen within the hensen cell region. however, the cholinesterase-staining fine fibres travelling along the floor of the tunnel to the base of the plaque are independent of the integrit ... | 1979 | 90498 |
| immunological similarities of mammalian xanthine oxidases. | the degree of relatedness among mammalian xanthine oxidases (xo) was determined by microcomplement fixation. rabbit anti bovine milk xo serum was tested against xanthine oxidase (homologous protein) and against the heterologous proteins of bovine liver, monkey liver, rat liver, lactating cow serum, nonlactating cow serum, and steer serum. the indices of dissimilarity for the heterologous proteins were expressed as units of immunological distance and the percent sequence differences among these p ... | 1979 | 90501 |
| unaltered catabolism of desialylated low-density lipoprotein in the pig and in cultured rat hepatocytes. | removal of the terminal sialic acid residues from many serum glycoproteins results in exposure of their penultimate galactose residues and rapid clearance from circulation by the liver. low-density lipoprotein is a glycoprotein containing 21 galactose and 9 sialic acid residues per particle. studies in this laboratory and others have shown that both the liver and extrahepatic tissues contribute to the degradation of low-density lipoprotein. this study was undertaken to determine whether desialyl ... | 1979 | 90503 |
| comparison of human alkaline phosphatase isoenzymes. structural evidence for three protein classes. | the structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(liver isoenzyme) serum, and the intestinal and placental isoenzymes cross-react with the anti-(placental ... | 1979 | 90505 |
| effect of glucocorticoids on the production of lactate dehydrogenase, malate dehydrogenase and alpha-fetoprotein by morris hepatoma 7777 in vitro. | | 1979 | 90506 |
| catalytic release of deoxyribonucleic acid bases by oxidation and reduction of an iron.bleomycin complex. | | 1979 | 90519 |
| enzymic o-glycosylation of synthetic peptides from sequences in basic myelin protein. | nine synthetic peptides containing sequences in the region of a threonine residue at position 98 of bovine basic myelin protein were prepared by the merrifield solid-phase method and tested for their ability to be glycosylated with [14c]uridinediphospho-n-acetylgalactosamine and a crude detergent-solubilized preparation of uridinediphospho-n-acetylgalactosamine:mucin polypeptide n-acetylgalactosaminyltransferase obtained from porcine submaxillary glands. the tetrapeptide thr-pro-pro-pro and all ... | 1979 | 90521 |
| circular dichroic analysis of the secondary structure of myelin basic protein and derived peptides bound to detergents and to lipid vesicles. | in aqueous solution bovine myelin basic protein exhibits no significant alpha-helical or beta-pleated sheet structure. however, in vivo this protein is associated largely with the myelin membrane: experiments have therefore been performed to determine the structure of the protein when bound to lipid bilayers. circular dichroism spectra show that this protein undergoes a major conformational change on binding to lipid bilayer vesicles formed from diacylphosphatidylserine or diacylphosphatidic aci ... | 1979 | 90525 |
| relations between fatty acids and oestrogen binding properties of pure rat alpha 1-foetoprotein. | a delipidation procedure based on treatment with charcoal at ph 3 has been applied to highly purified rat alpha 1-foetoprotein preparations. the oestrogen binding properties of the delipidated proteins have been studied with an equilibrium dialysis technique, and compared with the properties of the untreated foetal protein, as well as those of preparations reconstituted from the defatted alpha 1-foetoprotein and the removed lipids. an important increase has been evidenced for the binding levels ... | 1979 | 90528 |
| cells of origin of the spinocerebellar tract in the rat, studied with the method of retrograde transport of horseradish peroxidase. | following injections of horseradish peroxidase into the cerebellum, the distribution of labeled neurons was studied in the whole length of the spinal cord of the rat. to find the ascending side of the axons, injections were made following hemisections at c1 or between c1 and c2. labeled spinocerebellar tract neurons were classified into two groups according to the axonal course in the spinal cord; one is composed of neurons with uncrossed ascending axons and the other, neurons with crossed ascen ... | 1979 | 90539 |
| [effect of organochlorine pesticides on the ocular electric response of the white mouse]. | the injection of ddt or hch resulted in a decrease of the amplitude of b-wave of electroretinograms without any modification of its latency, whereas a-wave remained unchanged. in conclusion, we may say that organochlorous pesticides do not act on photoreceptors or synapses, but they act only on bipolar and ganglionnar neurones. | 1979 | 90547 |
| ultrastructural identification of non-adrenergic, non-cholinergic nerves in the rat anococcygeus muscle. | the innervation of the rat anococcygeus muscle has been investigated ultrastructurally following fixation with a modified chromaffin reaction for the demonstration of biogenic amines (tranzer and richards, 1976). three types of nerve profiles were revealed: (1) 60-70% of the profiles are adrenergic; (2) less than 5% of the profiles appear to be cholinergic; (3) up to 40% of the profiles are distinguished by the presence of a characteristically high proportion of electron-opaque, chromaffin-negat ... | 1979 | 90550 |
| cell-free synthesis and membrane insertion of mouse h-2dd histocompatibility antigen and beta 2-microglobulin. | messenger rna from sl2 lymphoma cells was translated in a cell-free system in the presence of microsomal membranes. mouse h-2dd histocompatibility antigen was correctly assembled in the microsomal membranes, and transmembrane insertion of the nascent chain was accompanied by glycosylation and cleavage of the signal sequence h-2kd antigens, synthesized in vivo, comprised a transmembrane glycoprotein and an unglycosylated protein in the cytoplasm. the glycosylated forms of the h-2dd and h-2kd anti ... | 1979 | 90552 |
| strain differences in tolerance induction to human gamma-globulin subclasses: dependence on macrophages. | | 1979 | 90559 |
| murine autoimmune hemolytic anemia induced via xenogeneic erythrocyte immunization. ii. antigenic specificity and clonal heterogeneity of the autoantibodies and their cellular correlates. | | 1979 | 90574 |
| in vitro studies of the replication of moloney murine leukemia virus. | | 1979 | 90575 |
| the effect of bleomycin on rat bone marrow cells. | experiments were carried out to determine the cytogenetic effects of the antibiotic bleomycin (blm) in rats using the bone marrow system. a total of eighteen male and eighteen female sprague-dawley rats were injected with varying concentration of blm, over varying periods of time. the results revealed that at low concentrations blm showed no alteration in the ploidy or the morphology of rat chromosomes. this however, was not the case when the dose or administration period was increased. | 1978 | 90585 |
| synaptonemal complexes of mouse and human pachytene chromosomes visualized by silver staining in air-dried preparations. | a new method is described which allows light microscopy visualization of the synaptonemal complexes in primary spermatocytes. the conventional acetic acid-alcohol fixed and air-dried preparations can be used for the modified silver staining technique. several hundred pachytene spermatocytes from a single mouse testis or human testicular biopsy can be examined. the prospective utilization of the present method is briefly discussed. | 1979 | 90592 |
| acute mouse and chronic dog toxicity studies of danthron, dioctyl sodium sulfosuccinate, poloxalkol and combinations. | because of an apparent typographic error in a us patent, there has been some confusion as to the acute oral toxicity of danthron and danthron in combination with dioctyl sodium sulfosuccinate (dss). acute oral toxicity studies in mice revealed ld50 values of greater than 7 gm/kg for danthron, 2.64 gm/kg for dss and 3.42 gm/kg for danthron/dss mixture (1:2 ratio). these results indicate that the lethality of these compounds is in the gm/kg range and not in the mg/kg range. a one year chronic toxi ... | 1977 | 90594 |
| hemopoietic stem cells bear sperm-associated antigens. | a fluorescence-activated cell sorter has been used to sort out enriched populations of murine hemopoietic stem cells from mouse bone marrow, based on fluorescence of cells labeled with various antisera. significant positive enrichment for stem cells was obtained with rabbit anti-human brain, human anti-human sperm and mouse anti-mouse f9 sera but not with mouse anti-mouse ia serum. these results support the hypothesis that the hemopoietic stem cell exhibits 'unique' surface antigens cross-reacti ... | 1979 | 90620 |
| the exogenous lipid requirement for histamine release from rat peritoneal mast cells stimulated by concanavalin a. | | 1979 | 90622 |
| identification of malondialdehyde as the tba-reactant formed by bleomycin-iron free radical damage to dna. | | 1979 | 90623 |
| [histological aspects of mycoses provoked by opportunistic fungi]. | the histological study with hotchkiss-mc manus staining and grocott silver impregnation of tissues invaded by opportunistic fungi is a rapid and easy means of diagnosis. the inflammatory reactions around the fungus give the certitude that it is a pathogen and not a contaminant. the major opportunists among canidida, aspergillus, mucor, absidia and cryptococcus species are presented in local and disseminated lesions, but all fungi, saprophytic in the normal host, can become pathogens in the immun ... | 1979 | 90626 |
| c1 inhibitor: the main inhibitor of human plasma kallikrein. | | 1978 | 90628 |
| immunological relatedness between beta-galactosidases of different bacterial origins. | | 1979 | 90646 |
| [animal experimental studies on the volume expanding effect of hydroxyethyl starch 40000 in the dog acute hemorrhagic shock]. | after acute hemorrhagic shock in dogs the hemodynamics of hydroxyethyl starch (mw: 40,000) are studied. the experiment shows that this plasma expander has a good efficiency for 3 to 4 hours. the indications of application of this colloidal solution are discussed. | 1979 | 90651 |
| [concerning the contribution by f. jesch, g. hübner, v. zumtobel, m. zimmermann and k. messmer, hydroxyethyl starch (has 450/0,7) in plasma and liver--course of concentration and human histologic changes [infusionstherapie 6:112-117 (1979)]. | | 1979 | 90652 |
| characterization of the antigenic determinants of cholera toxin subunits. | the antigenic specificity of purified preparations of a subunit, b subunit, alpha chain, and gamma chain of cholera toxin was studied by double immunodiffusion and radioimmunoassay with antisera produced in rabbits and mice. rabbits immunized with a subunit produced serum antibodies which were capable of binding radiolabeled a subunit, alpha chain, and b subunit. rabbits immunized with alpha chain produced serum antibodies that would bind radiolabeled alpha chain and a subunit. rabbits immunized ... | 1979 | 90654 |
| the indirect rat mast cell degranulation test reconsidered. | the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ... | 1979 | 90655 |
| molecular heterogeneity of alpha-fetoprotein secreted by primary and transplantable hepatocellular carcinomas. | | 1979 | 90663 |
| identification of a mycobacterium leprae specific protein antigen(s) and its possible application for the serodiagnosis of leprosy. | acetone-killed mycobacterium leprae separated from infected armadillo liver tissue without the use of proteases were treated with 0.2 m lithium acetate, 20 mm edta, ph 8.8 solution, and the concentrated antigen extract was analyzed by ouchterlony immunodiffusion. the antigen extract gave a single immunoprecipitate when reacted with pooled lepromatous leprosy (ll) patients sera made highly specific for m. leprae by adsorption. apparently identical precipitates were produced by reacting the antige ... | 1979 | 90666 |
| a structure in the wall of the internal carotid artery in the rat. | a structure has been found consistently in the wall of the internal carotid artery of the rat as it passes through the carotid canal. it consists of modified smooth muscle cells arranged longitudinally which exhibit abundant pinocytosis and are located close to endothelial cells, with which they make contact. these cells do not appear to be innervated. the closeness of this structure to the arterial lumen, into which it may actually protrude, suggests that it may be sensitive to vaso-active subs ... | 1979 | 90673 |
| purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity. | a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c.1.1.1.49) was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ... | 1979 | 90677 |
| conformation-specific antibodies as probes of the gamma-carboxyglutamic acid-rich region of bovine prothrombin. studies of metal-induced structural changes. | | 1979 | 90678 |
| radioimmunoassay system using a serovar-specific lipopolysaccharide antigen of leptospira. | the serovar-specific main (tm) antigen of leptospira interrogans serovar kremastos strain kyoto was labeled with sodium boro[3h]hydride after 1 h of oxidation with periodate. when 50 ng of the labeled compound was employed, 50% of the compound was bound to the antibodies contained in 7.5 x 10(-2) nl of anti-kremastos kyoto serum. under this condition, only 9 ng of the homologous tm antigen was required for 50% inhibition of serovar kremastos kyoto 3h-tm antigen-anti-kremastos kyoto serum binding ... | 1979 | 90682 |
| lymphocytes bearing receptors for ige. iii. transition of fc gamma r(+) cells to fc epsilon r(4) cells by ige. | | 1979 | 90699 |
| a radioimmune assay for human cupro-zinc superoxide dismutase and its application to erythrocytes. | a competition radioimmunoassay for human superoxide dismutase (sod-1) is described. radioiodinated sod-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using staphylococcus aureus. the assay is sensitive (detecting 5 ng enzyme) and reproducible. average values for sod-1 in lysates of erythrocytes from normal individuals is 854 +/- 100 ng/mg hemoglobin and that of patients with trisomy-21 is 1313 +/- 110 ng/mg hemoglobin. | 1979 | 90710 |
| on the kinetics and maximal capacity of the system for rapid axonal transport in mammalian neurones. | 1. rabbit peroneal nerves were incubated in vitro in two-compartment chambers. step-gradients of temperature were established so that the proximal part of each nerve was slightly warmer than the distal part. after incubation, the distribution of dopamine-beta-hydroxylase (dbh) activity along the nerves was examined as an indication of the behaviour of rapid transport in adrenergic axons. 2. with temperature gradients of 5 and 8 degrees c, transport velocity in the proximal regions was expected f ... | 1979 | 90719 |
| assessment of blood leukocyte microbial killing by using a new fluorochrome microassay. | | 1979 | 90723 |
| proteolytic degradation of human erythrocyte band 3 by membrane-associated protease activity. | antisera directed against the cytoplasmic portion of human erythrocyte band 3 were used to follow the degradation of the band 3 molecule. small amounts of band 3 were degraded when well-washed red cell membrane ghosts were incubated in the cold; this process was greatly accelerated by incubating ghosts were incubated in the cold; this process was greatly accelerated by incubating ghosts at 37 degrees c. band 3 labeled with pyridoxal-phosphate was digested at comparable rates. band 3 digestion al ... | 1979 | 90727 |
| [neural secretion of histamine by the gastric mucosa and its effects on gastric motility]. | | 1978 | 90747 |
| c19o2-steroid transformations in the human normal, hyperplastic and cancerous prostate. | | 1979 | 90752 |
| age-dependent variability of ribosomal rna-gene activity in man as determined from frequencies of silver staining nucleolus organizing regions on metaphase chromosomes of lymphocytes and fibroblasts. | frequencies of silver staining nucleolus organizing regions (nors) have been determined in lymphocytes and fibroblasts from very young and from aged persons. since silver staining of nors is associated with activity of ribosomal rna-genes, we used this approach to investigate a possible inactivation of these genes during aging. our lymphocyte data are based on a study per age-group of 220 metaphases from 10 subjects. although in both age-groups modal numbers of silver staining chromosomes per me ... | 1979 | 90756 |
| prolonged survival of corneal allografts in rabbits treated with cyclosporin a. | | 1979 | 90771 |