| isolation of aerobic, gliding, xylanolytic and laminarinolytic bacteria from acidic sphagnum peatlands and emended description of chitinophaga arvensicola kampfer et al. 2006. | four aerobic, heterotrophic, yellow-pigmented and flexirubin-producing bacterial strains with gliding motility were isolated from acidic sphagnum-dominated wetlands of northern russia. these bacteria are capable of degrading xylan, laminarin and some other polysaccharides, but not cellulose, pectin or chitin. the four strains possess almost identical 16s rrna gene sequences and are most closely related (98.9-99.5 % sequence similarity) to the recently reclassified species of the phylum bacteroid ... | 2006 | 17158974 |
| bacterial growth and community composition in fractions of dissolved organic carbon of different molar mass from interstitial water. | the ability of different molar-mass fractions of interstitial dissolved organic carbon (doc) from a small stream to stimulate bacterial growth was determined. growth was the greatest in the low-molar mass (<1 kda) (lmm) fraction and the least in the high-molar mass (>10 kda) (hmm) one. the lmm fraction consistently had a higher ratio of carbon to organic nitrogen than other fractions. the fluorescence in situ hybridization analysis revealed that bacterial populations in interstitial water were d ... | 2006 | 17176764 |
| volatile organic compounds from arctic bacteria of the cytophaga-flavobacterium-bacteroides group: a retrobiosynthetic approach in chemotaxonomic investigations. | volatile organic compounds emitted by different marine arctic strains of the cytophaga-flavobacterium-bacteroides group were investigated by using a modified closed-loop stripping apparatus (clsa). seven of nine strains emitted volatiles, dominated by methyl ketones, in specific patterns. the methyl ketones were aliphatic saturated, or unsaturated, and comprised 12 to 18 c-atoms, sometimes with terminal me branches. they were identified by gc/ms, retention-index calculations, derivatization with ... | 2005 | 17191983 |
| plant-microbe association for rhizoremediation of chloronitroaromatic pollutants with comamonas sp. strain cnb-1. | comamonas sp. strain cnb-1, isolated from activated sludge and having a strong ability to degrade 4-chloronitrobenzene (4cnb), was applied for rhizoremediation of 4cnb-polluted soil through association with alfalfa. confocal laser scanning microscopy revealed that strain cnb-1 successfully colonized alfalfa roots. determination of strain cnb-1 populations by cultivation method and by quantitative competitive pcr technique targeting the chloronitrobenzene nitroreductase gene showed that the popul ... | 2007 | 17222144 |
| modulation of microbial predator-prey dynamics by phosphorus availability: growth patterns and survival strategies of bacterial phylogenetic clades. | we simultaneously studied the impact of top-down (protistan grazing) and bottom-up (phosphorus availability) factors on the numbers and biomasses of bacteria from various phylogenetic lineages, and on their growth and activity parameters in the oligo-mesotrophic piburger see, austria. enhanced grazing resulted in decreased proportions of bacteria with high nucleic acid content (high-na bacteria) and lower detection rates by fish. there was a change in the composition of the bacterial assemblage, ... | 2007 | 17250752 |
| investigation of total bacterial and ammonia-oxidizing bacterial community composition in a full-scale aerated submerged biofilm reactor for drinking water pretreatment in china. | the community composition of total bacteria and ammonia-oxidizing bacteria in a full-scale aerated submerged biofilm reactor for drinking water pretreatment was characterized by analysis of 16s rrna gene and the functional gene amoa, respectively. sampling was performed in february and in july. 16s rrna gene clone libraries revealed 13 bacterial divisions. at both sampling dates, the majority of clone sequences were related to the alpha- and betaproteobacteria. a minor proportion belonged to the ... | 2007 | 17263855 |
| the leading role of actinobacteria in aerobic cellulose degradation in sphagnum peat bogs. | | 2006 | 17278855 |
| synergistic and antagonistic effects of viral lysis and protistan grazing on bacterial biomass, production and diversity. | in a mesotrophic reservoir, we examined the effects on the bacterioplankton of distinct consumers of bacteria, viruses and heterotrophic nanoflagellates, both alone and combined in an experiment using natural populations and in situ incubations in dialysis bags. ribosomal rna-targeted probes were employed as well as 16s rna gene based pcr denaturing gradient gel electrophoresis (dgge) to enumerate bacterial groups and assess bacterial community composition. we employed probes for actinobacteria ... | 2007 | 17298376 |
| nutrients and other abiotic factors affecting bacterial communities in an ohio river (usa). | nitrogen and phosphorus additions from anthropogenic sources can alter the nutrient pool of aquatic systems, both through increased nutrient concentrations and changes in stoichiometry. because bacteria are important in nutrient cycling and aquatic food webs, information about how nutrients affect bacterial communities enhances our understanding of how changes in nutrient concentrations and stoichiometry potentially affect aquatic ecosystems as a whole. in this study, bacterial communities were ... | 2007 | 17308951 |
| characterization of the microbial diversity in a permafrost sample from the canadian high arctic using culture-dependent and culture-independent methods. | a combination of culture-dependent and culture-independent methodologies (bacteria and archaea 16s rrna gene clone library analyses) was used to determine the microbial diversity present within a geographically distinct high arctic permafrost sample. culturable bacteria isolates, identified by 16s rrna gene sequencing, belonged to the phyla firmicutes, actinobacteria and proteobacteria with spore-forming firmicutes being the most abundant; the majority of the isolates (19/23) were psychrotoleran ... | 2007 | 17313587 |
| actively growing bacteria in the inland sea of japan, identified by combined bromodeoxyuridine immunocapture and denaturing gradient gel electrophoresis. | a fundamental question in microbial oceanography concerns the relationship between prokaryote diversity and biogeochemical function in an ecosystem context. we combined bromodeoxyuridine (brdu) magnetic bead immunocapture and pcr-denaturing gradient gel electrophoresis (bump-dgge) to examine phylotype-specific growth in natural marine assemblages. we also examined a broad range of marine bacterial isolates to determine their abilities to incorporate brdu in order to test the validity of the meth ... | 2007 | 17337555 |
| molecular characterization of bacterial endosymbionts of acanthamoeba isolates from infected corneas of korean patients. | the endosymbionts of 4 strains of acanthamoeba (ka/e9, ka/e21, ka/e22, and ka/e23) isolated from the infected corneas of korean patients were characterized via orcein stain, transmission electron microscopic examination, and 16s rdna sequence analysis. double membrane-bound, rod-shaped endosymbionts were distributed randomly throughout both the trophozoites and cysts of each of acanthamoeba isolates. the endosymbionts of ka/e9, ka/e22, and ka/e23 were surrounded by electron-translucent areas. no ... | 2007 | 17374972 |
| bacterial community analysis of shallow groundwater undergoing sequential anaerobic and aerobic chloroethene biotransformation. | at department of energy site 300, beneficial hydrocarbon cocontaminants and favorable subsurface conditions facilitate sequential reductive dechlorination of trichloroethene (tce) and rapid oxidation of the resultant cis-dichloroethene (cis-dce) upon periodic oxygen influx. we assessed the geochemistry and microbial community of groundwater from across the site. removal of cis-dce was shown to coincide with oxygen influx in hydrocarbon-containing groundwater near the source area. principal compo ... | 2007 | 17386036 |
| genome sequence of the cellulolytic gliding bacterium cytophaga hutchinsonii. | the complete dna sequence of the aerobic cellulolytic soil bacterium cytophaga hutchinsonii, which belongs to the phylum bacteroidetes, is presented. the genome consists of a single, circular, 4.43-mb chromosome containing 3,790 open reading frames, 1,986 of which have been assigned a tentative function. two of the most striking characteristics of c. hutchinsonii are its rapid gliding motility over surfaces and its contact-dependent digestion of crystalline cellulose. the mechanism of c. hutchin ... | 2007 | 17400776 |
| characterization of a sulfide-oxidizing biofilm developed in a packed-column reactor. | the potential of microbial mats to develop sulfide-oxidizing biofims was explored. a bioreactor specially designed for the treatment of sulfide-containing effluents was inoculated with a microbial-mat sample, and a complex microbial biofilm with sulfide-oxidation activity developed. the microbial composition of the biofilm was studied by pigment, microscopy, and 16s rrna gene analyses. purple sulfur bacteria and diatoms were observed by microscopy, chlorophyll a and bacteriochlorophyll a were de ... | 2007 | 17407058 |
| epilithic cyanobacterial communities of a marine tropical beach rock (heron island, great barrier reef): diversity and diazotrophy. | the diversity and nitrogenase activity of epilithic marine microbes in a holocene beach rock (heron island, great barrier reef, australia) with a proposed biological calcification "microbialite" origin were examined. partial 16s rrna gene sequences from the dominant mat (a coherent and layered pink-pigmented community spread over the beach rock) and biofilms (nonstratified, differently pigmented microbial communities of small shallow depressions) were retrieved using denaturing gradient gel elec ... | 2007 | 17416688 |
| identification, synthesis, and conformation of tri- and tetrathiacycloalkanes from marine bacteria. | seven new cyclic natural polysulfides 1-7 were identified in extracts of two bacterial cytophaga strains (cfb-phylum) isolated from biofilms from the north sea. their structures are based on mono- and dimeric-cyclization products of 2-methylpropane-1,2-dithiol 8, which was also present in the extract in trace amounts. the structures were deduced by analysis of their mass spectra and confirmed by synthesis. the 1h nmr spectra of some these compounds suggested a high flexibility of the trithiepane ... | 2007 | 17428096 |
| characterization of the inhabitancy of mouse intestinal bacteria (mib) in rodents and humans by real-time pcr with group-specific primers. | mouse intestinal bacteria (mib) is a new operational taxonomic unit (otu) belonging to the bacteroides subgroup in the cytophaga-flavobacter-bacteroides (cfb) phylum recently found in the intestine of mice, rats and humans. however, their characters are still unknown since they have not yet been isolated by culture. to understand their habitat characteristics in intestinal tracts, the quantification assays of mib were established using mib group-specific primers. the mib population in the intest ... | 2007 | 17446674 |
| adherent bacteria in heavy metal contaminated marine sediments. | the eubacterial communities adherent to sediment particles were studied in heavy metal contaminated coastal sediments. six sampling sites on the belgian continental plate and presenting various metal loads, granulometries, and organic matter content, were compared. the results indicated that the total microbial biomass (attached + free-living bacteria) was negatively correlated to hcl-extractable metal levels (p<0.05) and that the percentage of cells adherent to sediment particles was close to 1 ... | 2007 | 17453724 |
| combinatorial materials research applied to the development of new surface coatings iii. utilisation of a high-throughput multiwell plate screening method to rapidly assess bacterial biofilm retention on antifouling surfaces. | the authors recently reported on the development of a novel multiwell plate screening method for the high-throughput assessment of bacterial biofilm retention on surfaces. two series of biocide containing coatings were prepared to assess the ability of the developed assay to adequately discern differences in antifouling performance: i) a commercially available poly(methyl methacrylate) (pmma) and silicone elastomer (dc) physically blended with an organic antifouling biocide sea-nine 211 (sn211) ... | 2007 | 17453727 |
| combinatorial materials research applied to the development of new surface coatings iv. a high-throughput bacterial biofilm retention and retraction assay for screening fouling-release performance of coatings. | a high-throughput bacterial biofilm retention screening method has been augmented to facilitate the rapid analysis and down-selection of fouling-release coatings for identification of promising candidates. coatings were cast in modified 24-well tissue culture plates and inoculated with the marine bacterium cytophaga lytica for attachment and biofilm growth. biofilms retained after rinsing with deionised water were dried at ambient laboratory conditions. during the drying process, retained biofil ... | 2007 | 17453728 |
| effect of salinity changes on the bacterial diversity, photosynthesis and oxygen consumption of cyanobacterial mats from an intertidal flat of the arabian gulf. | the effects of salinity fluctuation on bacterial diversity, rates of gross photosynthesis (gp) and oxygen consumption in the light (ocl) and in the dark (ocd) were investigated in three submerged cyanobacterial mats from a transect on an intertidal flat. the transect ran 1 km inland from the low water mark along an increasingly extreme habitat with respect to salinity. the response of gp, ocl and ocd in each sample to various salinities (65 per thousand, 100 per thousand, 150 per thousand and 20 ... | 2007 | 17504476 |
| filter-feeder macroinvertebrates as key players in culturable bacteria biodiversity control: a case of study with sabella spallanzanii (polychaeta: sabellidae). | the present study investigates the effect of the filtering activity of sabella spallanzanii on the culturable heterotrophic bacterial community through the comparison of the bacterial diversity in transplanted polychaetes and the surrounding seawater. for isolation of culturable heterotrophic bacteria, seawater samples as well as polychaete homogenates were plated in triplicates onto bacto marine agar 2216 (difco). all the colonies grown were isolated, subcultured and identified by several morph ... | 2007 | 17548107 |
| the diversity of bacterial communities associated with atlantic cod gadus morhua. | the spatial and temporal changes in the bacterial communities associated with the atlantic cod gadus morhua were investigated using terminal restriction fragment length polymorphism (t-rflp) analysis of 16s recombinant dna (rdna). epidermal mucous was sampled from 366 cod caught in three harvest locations (baltic, icelandic, and north seas) over three seasons (spring 2002, autumn 2002, and spring 2003), and an automated method for the high-throughput processing of environmental samples was devel ... | 2008 | 17624487 |
| chitinophaga ginsengisegetis sp. nov. and chitinophaga ginsengisoli sp. nov., isolated from soil of a ginseng field in south korea. | two novel strains belonging to the phylum bacteroidetes [formerly the cytophaga-flexibacter-bacteroides (cfb) group], designated gsoil 040(t) and gsoil 052(t), were isolated from the soil of a ginseng field in pocheon province, south korea. a polyphasic approach was used to characterize the taxonomic position of the novel strains. both strains were gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. phylogenetic analysis based on 16s rrna gene sequences indicated that the novel ... | 2007 | 17625164 |
| molecular cloning and biochemical characterization of a family-9 endoglucanase with an unusual structure from the gliding bacteria cytophaga hutchinsonii. | cytophaga hutchinsonii was originally isolated from sugarcane piles. this microorganism therefore probably produces an array of enzymes allowing it to digest cellulosic substrates. c. hutchinsonii thus represents a rich source of potentially effective cellulase enzymes that can be harnessed for conversion of biomass to simple sugars. these sugars can then be used as feedstock for ethanol production or other chemical syntheses. in this study, we report the pcr cloning of an endoglucanase gene (ce ... | 2007 | 17625271 |
| [lignite microorganisms]. | the first demonstration that samples of lignite at a depth of 10 m are considerably enriched in bacteria is reported. according to direct microscopy, the abundance of bacteria was about 10(7) cells/g. about 70% of cells had intact cell membranes and small size, which points to their anabiotic state. the fungal mycelium length was no more than 1 m. lignite inoculation onto solid glucose-yeast-peptone medium allowed as to isolate bacteria of the genera bacillus, rhodococcus, arthrobacter, micrococ ... | 2007 | 17668464 |
| characterization of bacterial diversity in two aerated lagoons of a wastewater treatment plant using pcr-dgge analysis. | aerated lagoons are commonly used for domestic and industrial wastewater treatment due to their low cost and minimal need of operational requirements. however, little information is known regarding microbial communities that inhabit these ecosystems. in this study, a 16s-dgge approach was used to estimate bacterial diversity and to monitor community changes in two aerated lagoons from a wastewater treatment plant receiving urban and industrial effluents. pronounced shifts between bacterial commu ... | 2009 | 17681740 |
| endophytic bacterial diversity in rice (oryza sativa l.) roots estimated by 16s rdna sequence analysis. | the endophytic bacterial diversity in the roots of rice (oryza sativa l.) growing in the agricultural experimental station in hebei province, china was analyzed by 16s rdna cloning, amplified ribosomal dna restriction analysis (ardra), and sequence homology comparison. to effectively exclude the interference of chloroplast dna and mitochondrial dna of rice, a pair of bacterial pcr primers (799f-1492r) was selected to specifically amplify bacterial 16s rdna sequences directly from rice root tissu ... | 2008 | 17690836 |
| annual dynamics of bacterioplankton assemblages in the gulf of trieste (northern adriatic sea). | bacterioplankton community diversity was investigated monthly in coastal waters of the gulf of trieste (ne adriatic sea) throughout 2003. superficial bacterial assemblages of two differently freshwater influenced stations were studied using pcr-dgge fingerprinting techniques. bacterial genetic diversity of the sampled area, as estimates of the number of dgge bands was high (36-64) compared to that reported in other studies employing this fingerprint technique. the similarity index (sorensen inde ... | 2007 | 17728077 |
| substrate degradation kinetics, microbial diversity, and current efficiency of microbial fuel cells supplied with marine plankton. | the decomposition of marine plankton in two-chamber, seawater-filled microbial fuel cells (mfcs) has been investigated and related to resulting chemical changes, electrode potentials, current efficiencies, and microbial diversity. six experiments were run at various discharge potentials, and a seventh served as an open-circuit control. the plankton consisted of a mixture of freshly captured phytoplankton and zooplankton (0.21 to 1 mm) added at an initial batch concentration of 27.5 mmol liter(-1 ... | 2007 | 17766447 |
| quantitative analysis of biofilm eps uronic acid content. | the uronic acids assay was evaluated for its ability to measure the amount of uronic acids contained within a biofilm exopolysaccharide matrix. cytophaga lytica, a marine bacterium isolated from a naturally occurring biofilm, was used to form single-species biofilms for the method assessment. the assay was found to be simple, reproducible, and sensitive to 1 microg levels, suggesting its potential for application as a screening technique for compounds that inhibit the production of microbial bio ... | 2007 | 17822791 |
| characterization of microbial communities in a pilot-scale constructed wetland using plfa and pcr-dgge analyses. | phospholipid fatty acid (plfa) analysis and 16s ribosomal dna polymerase chain reaction amplification-denaturing gradient gel electrophoresis (pcr-dgge) were used to determine microbial communities and predominant microbial populations in water samples collected from a pilot-scale constructed wetland system. this pilot-scale constructed wetland system consists of three types: subsurface-flow (ssf), surface-flow (sf) and a floating aquatic plant (fap) system. analysis of plfa profiles indicated p ... | 2007 | 17849306 |
| phylogenetic diversity of bacteria isolates from the qinghai-tibet plateau permafrost region. | the qinghai-tibet plateau in east asia is a unique and important permafrost environment. however, its microbiology remains largely unexplored to date. in this study, sediment samples were collected from the qinghai-tibet plateau permafrost region, bacteria isolation procedures were performed 8 times, and the samples incubated at 4 degrees c for nearly 3 months. the number of colony forming units (cfu) ranged from 0 to 10(7)/(g dry soil). the quantity of culturable bacteria grew exponentially wit ... | 2007 | 17898857 |
| fate and distribution of brevetoxin (pbtx) following lysis of karenia brevis by algicidal bacteria, including analysis of open a-ring derivatives. | flavobacteriaceae (strain s03) and cytophaga sp. (strain 41-dbg2) are algicidal bacteria active against the brevetoxin (pbtx)-producing, red tide dinoflagellate, karenia brevis. little is known about the fate of pbtx associated with k. brevis cells following attack by such bacteria. the fate and distribution of pbtx in k. brevis cultures exposed to these algicidal strains were thus examined by receptor binding assay and liquid chromatography/mass spectrometry (lc/ms) in three size fractions (>5, ... | 2007 | 17905402 |
| microbiological study of the body wall lesions of the echinoid tripneustes gratilla. | the microbiota of the body wall lesions of the echinoid tripneustes gratilla, initiated by the grazing action of the parasitic gastropod vexilla vexillum, was investigated with a pluridisciplinary approach. parasitised sea urchins showed body wall lesions strongly infected by bacteria that progressed through the test and reached the coelomic cavity after ca. 1 mo. we report here on the bacterial community observed in lesions of echinoids collected in situ and on the bacteria that successively ap ... | 2007 | 17933399 |
| biostimulation of estuarine microbiota on substrate coated agar slides: a novel approach to study diversity of autochthonous bdellovibrio- and like organisms. | characterization of bdellovibrio- and like organisms (balos) from environmental samples involves growing them in the presence of gram-negative prey bacteria and isolation of balo plaques. this labor-intensive enrichment and isolation procedure may impede the detection and phylogenetic characterization of uncultivable balos. in this article, we describe a simple slide biofilm assay to improve detection and characterization of balo microbiota. agar spiked with biostimulants such as yeast extract ( ... | 2008 | 17968612 |
| [characteristics of adhesion of epiphytic bacteria on leaves of the seagrass zostera marina and on abiotic surfaces]. | a comparative study of the adhesion of epiphytic bacteria and marine free-living, saprophytic, and pathogenic bacteria on seagrass leaves and abiotic surfaces was performed to prove the occurrence of true epiphytes of zostera marina and to elucidate the bacterium-plant symbiotrophic relationships. it was shown that in the course of adhesion to the seagrass leaves of two taxonomically different bacteria, cytophaga sp. kmm 3552 and pseudoalteromonas citrea kmm 461, isolated from the seagrass surfa ... | 2007 | 17974207 |
| short-term temporal variability in airborne bacterial and fungal populations. | airborne microorganisms have been studied for centuries, but the majority of this research has relied on cultivation-dependent surveys that may not capture all of the microbial diversity in the atmosphere. as a result, our understanding of airborne microbial ecology is limited despite the relevance of airborne microbes to human health, various ecosystem functions, and environmental quality. cultivation-independent surveys of small-subunit rrna genes were conducted in order to identify the types ... | 2008 | 17981945 |
| the diversity of the bacterial communities associated with the azooxanthellate hexacoral cirrhipathes lutkeni. | this study examined the symbiotic microbiota of the hexacoral cirrhipathes lutkeni using traditional plate culture, fluorescence in situ hybridization (fish) and 16s rdna characterization. fish counts for the whole coral (holobiont) showed a major presence of gamma-proteobacteria (22%) and actinobacteria (19%), followed by alpha-proteobacteria (14%), firmicutes (9%), cytophaga-flavobacterium (7%), beta-proteobacteria (6%) and chloroflexi (2%). in contrast to the diversity observed by fish, plate ... | 2007 | 18043672 |
| chitinophaga soli sp. nov. and chitinophaga terrae sp. nov., isolated from soil of a ginseng field in pocheon province, korea. | two novel strains of the cytophaga-flexibacter-bacteroides (cfb) group, designated gsoil 219" and gsoil 2381, were isolated from soil of a ginseng field of pocheon province in korea. both strains were gram-negative, aerobic, nonmotile, nonspore-forming, and rod-shaped. phylogenetic analysis based on 16s rrna gene sequences indicated that both isolates belong to the genus chitinophaga but were clearly separated from established species of this genus. the sequence similarities between strain gsoil ... | 2007 | 18051289 |
| quorum sensing by n-acylhomoserine lactones is not required for aeromonas hydrophila during growth with organic particles in lake water microcosms. | it was investigated whether quorum sensing (qs) mediated by n-acylhomoserine lactones (ahls) was important for heterotrophic bacteria from the littoral zone of the oligotrophic lake constance for growth with organic particles. more than 900 colonies from lake water microcosms with artificial organic aggregates consisting of autoclaved unicellular algae embedded in agarose beads were screened for ahl-production. ahl-producing bacteria of the genus aeromonas enriched in the microcosms but ahls cou ... | 2008 | 18066531 |
| bacterial population structure of the jute-retting environment. | jute is one of the most versatile bast fibers obtained through the process of retting, which is a result of decomposition of stalks by the indigenous microflora. however, bacterial communities associated with the retting of jute are not well characterized. to investigate the presence of microorganisms during the process of jute retting, full-cycle rrna approach was followed, and two 16s rrna gene libraries, from jute-retting locations of krishnanagar and barrackpore, were constructed. phylotypes ... | 2008 | 18097714 |
| community structures of actively growing bacteria shift along a north-south transect in the western north pacific. | bacterial community structures and their activities in the ocean are tightly coupled with organic matter fluxes and thus control ocean biogeochemical cycles. bromodeoxyuridine (brdu), halogenated nucleoside and thymidine analogue, has been recently used to monitor actively growing bacteria (agb) in natural environments. we labelled dna of proliferating cells in seawater bacterial assemblages with brdu and determined community structures of the bacteria that were possible key species in mediating ... | 2008 | 18177366 |
| planktonic bacteria and fungi are selectively eliminated by exposure to marine macroalgae in close proximity. | to test whether macroalgae affect microbial colonizers in close proximity in a phylum-specific fashion, the community richness of planktonic bacteria and fungi was analyzed with selective oligonucleotide probes targeting the cytophaga/flavobacterium/bacteroides (cfb), alphaproteobacteria and roseobacter group and the its1 region of marine fungi. naturally occuring planktonic microorganisms were incubated in the presence of macroalgae or in seawater previously conditioned with macroalgal metaboli ... | 2008 | 18194343 |
| major differences of bacterial diversity and activity inside and outside of a natural iron-fertilized phytoplankton bloom in the southern ocean. | one of the first comparisons of a natural iron fertilized bloom with a high-nutrient low-chlorophyll (hnlc) site was undertaken during the kerguelen ocean and plateau compared study (keops) cruise. to understand better the bacteria-phytoplankton relationship in the context of natural iron fertilization, bacterial diversity and activity was investigated in the bloom and in the adjacent hnlc region by 16s rdna clone libraries and by single strand conformation polymorphism (sscp) analysis. both lib ... | 2008 | 18237307 |
| biodegradation potential and bacterial diversity of a petrochemical wastewater treatment plant in iran. | an activated sludge treatment was evaluated for its effectiveness in cleaning up a petrochemical wastewater in iran. for assessing biodegradation potential of activated sludge, seven characteristics of wastewater (temperature, ph, dissolved oxygen, chemical oxygen demand, concentrations of ethylene dichloride, vinyl chloride, and total hydrocarbons) were monitored during six months. it was shown that dominant pollutants in order of magnitude were normal-alkanes (c(10)-c(21)), aromatics, and poly ... | 2008 | 18255283 |
| [study on microbial diversity and community in miaoergou snow of east tianshan mountains and their relation to climatic and environmental changes]. | the different depth snow samples were collected from the miaoergou glacier in east tianshan mountains regions, china. total bacteria counts were established by 4',6- diamino-2-phenylindole (dapi). both culture-dependent and culture-independent methods, denaturing gradient gel electrophoresis (dgge), were used to examine the bacterial diversity and community structure. the microbial abundance and diversity index have a close relationship with mineral particle concentration. these mineral particle ... | 2007 | 18271257 |
| functional responses of prokaryotes and viruses to grazer effects and nutrient additions in freshwater microcosms. | for aquatic systems, there is little data on the interactions between viruses, prokaryotes, grazers and the availability of resources. we conducted a microcosm experiment using a size fractionation approach to manipulate grazers, with a purpose to examine the effects of inorganic and organic nutrients on viral and prokaryotic standing stocks and activities, and on prokaryotic community composition as assessed by fluorescent in situ hybridization (fish) method. experiments were performed during p ... | 2008 | 18273065 |
| characterization of cytophaga-flavobacteria community structure in the bering sea by cluster-specific 16s rrna gene amplification analysis. | a newly designed cytophaga-flavobacteria-specific 16s rrna gene primer pair was employed to investigate the cf community structure in the bering sea, revealing a previously unknown and unexpected high cf diversity in this high latitude cold sea. in total, 56 clones were sequenced and 50 unique cf 16 rrna gene fragments were obtained, clustering into 16 cf subgroups, including nine cosmopolitan subgroups, five psychrophilic subgroups, and two putatively autochthonous subgroups. the majority of se ... | 2008 | 18309261 |
| characterization, identification, and cloning of the s-layer protein from cytophaga sp. | we characterized, identified, and cloned a major protein which comprised 16% of the total proteins from cytophaga sp. cell lysate. after french pressing, the fraction of cell envelope was treated with 0.2% triton x-100 to remove cell membranes. subsequent sds-page analysis of the triton x-100-insoluble cell wall revealed a protein of 120 kda with a pi of 5.4, which was identified by gold immunostaining as the surface (s)-layer protein of this soil bacterium. the nucleotide sequence of the cloned ... | 2008 | 18322733 |
| phylogenetic diversity of bacteria associated with the mucus of red sea corals. | coral reefs are the most biodiverse and biologically productive of all marine ecosystems. corals harbor diverse and abundant prokaryotic communities. however, little is known about the diversity of coral-associated bacterial communities. mucus is a characteristic product of all corals, forming a coating over their polyps. the coral mucus is a rich substrate for microorganisms. mucus was collected with a procedure using sterile cotton swabs that minimized contamination of the coral mucus by surro ... | 2008 | 18355296 |
| bacteria associated with toxic clonal cultures of the dinoflagellate ostreopsis lenticularis. | the marine toxic dinoflagellate ostreopsis lenticularis has been implicated as the major vector in ciguatera seafood poisoning on the southwest coast of puerto rico. studies have demonstrated that associated bacteria play a role in the ciguatoxin production and that different clonal cultures of o. lenticularis harbor different culturable bacteria. in this study, more than 125 associated bacteria from two toxic clonal cultures of o. lenticularis (no. 302 and no. 303) were analyzed utilizing polym ... | 2008 | 18365282 |
| thermally treated grass fibers as colonizable substrate for beneficial bacterial inoculum. | this study investigates how thermally treated (i.e., torrefied) grass, a new prospective ingredient of potting soils, is colonized by microorganisms. torrefied grass fibers (tgf) represent a specific colonizable niche, which is potentially useful to establish a beneficial microbial community that improves plant growth. tgf and torrefied grass extracts (tge) were inoculated with a suspension of microorganisms obtained from soil. sequential microbial enrichment steps were then performed in both su ... | 2008 | 18368438 |
| three microbial strategies for plant cell wall degradation. | cellulolytic bacteria and fungi have been shown to use two different approaches to degrade cellulose. most aerobic microbes secrete sets of individual cellulases, many of which contain a carbohydrate binding molecule (cbm), which act synergistically on native cellulose. most anaerobic microorganisms produce large multienzyme complexes called cellulosomes, which are usually attached to the outer surface of the microorganism. most of the cellulosomal enzymes lack a cbm, but the cohesin subunit, to ... | 2008 | 18378599 |
| vertical distribution and phylogenetic composition of bacteria in the eastern tropical north pacific ocean. | the vertical community structure of bacteria along a depth profile in the eastern tropical north pacific ocean (13 degrees n, 104 degrees w) was studied by flow cytometry measurement and 16s rrna gene clone libraries analysis. picoeukaryotes and synechococcus peaked at 30 m and decreased sharply below 50 m, while prochlorococcus peaked at both 30 and 100 m layers and disappeared below 200 m. heterotrophic bacteria peaked above shallow thermocline and decreased along the depth profile. sequences ... | 2009 | 18387795 |
| epiphytic bacterial community composition on two common submerged macrophytes in brackish water and freshwater. | plants and their heterotrophic bacterial biofilm communities possibly strongly interact, especially in aquatic systems. we aimed to ascertain whether different macrophytes or their habitats determine bacterial community composition. we compared the composition of epiphytic bacteria on two common aquatic macrophytes, the macroalga chara aspera willd. and the angiosperm myriophyllum spicatum l., in two habitats, freshwater (lake constance) and brackish water (schaproder bodden), using fluorescence ... | 2008 | 18402668 |
| molecular structure of endotoxins from gram-negative marine bacteria: an update. | marine bacteria are microrganisms that have adapted, through millions of years, to survival in environments often characterized by one or more extreme physical or chemical parameters, namely pressure, temperature and salinity. the main interest in the research on marine bacteria is due to their ability to produce several biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents. nonetheless, lipopolysaccharides (lpss), or their portions, from g ... | 2007 | 18463721 |
| diversity and abundance of nitrate assimilation genes in the northern south china sea. | marine heterotrophic microorganisms that assimilate nitrate play an important role in nitrogen and carbon cycling in the water column. the nasa gene, encoding the nitrate assimilation enzyme, was selected as a functional marker to examine the nitrate assimilation community in the south china sea (scs). pcr amplification, restriction fragment length polymorphism (rflp) screening, and phylogenetic analysis of nasa gene sequences were performed to characterize in situ nitrate assimilatory bacteria. ... | 2008 | 18481138 |
| analysis of the phylogenetic diversity of estrone-degrading bacteria in activated sewage sludge using microautoradiography-fluorescence in situ hybridization. | in situ uptake of [2,4,6,7-3h(n)]estrone ([3h]e1) by the major phylogenetic groups present in activated sludge samples from two different municipal wastewater treatment plants was investigated using microautoradiography-fluorescence in situ hybridization (mar-fish). approximately 1-2% of the total cells confined in the samples by an eub probe mix contributed to e1 assimilation. almost all the detected e1-assimilating cells involved in the early phase of e1 degradation were affiliated with the be ... | 2008 | 18513907 |
| spatial and temporal patterns in the microbial diversity of a meromictic soda lake in washington state. | the microbial community diversity and composition of meromictic soap lake were studied using culture-dependent and culture-independent approaches. the water column and sediments were sampled monthly for a year. denaturing gradient gel electrophoresis of bacterial and archaeal 16s rrna genes showed an increase in diversity with depth for both groups. late-summer samples harbored the highest prokaryotic diversity, and the bacteria exhibited less seasonal variability than the archaea. most-probable ... | 2008 | 18552187 |
| synthesis and regulation of extracellular beta (1-3) glucanase and protease by cytophaga sp. in batch and continuous culture. | lytic enzyme systems with the ability to break whole cells of yeast are a mixture of several enzymes and virtually all contain beta(1-3)glucanases and some protease. it appears that the presence of these two enzyme activities is necessary to break the two layers of the rigid cell wall. the enzyme system of cytophaga ncib 9497 has a high activity towards the walls of yeast and also of bacteria. this article describes the production of this extracellular lytic enzyme system in batch and continuous ... | 1986 | 18561226 |
| automated image-based method for laboratory screening of coating libraries for adhesion of algae and bacterial biofilms. | assessment and down-selection of non-biocidal coatings that prevent the adhesion of fouling organisms in the marine environment requires a hierarchy of laboratory methods to reduce the number of experimental coatings for field testing. automated image-based methods are described that facilitate rapid, quantitative biological screening of coatings generated through combinatorial polymer chemistry. algorithms are described that measure the coverage of bacterial and algal biofilms on coatings prepa ... | 2008 | 18564880 |
| kinetics of enzymatic lysis and disruption of yeast cells: i. evaluation of two lytic systems with different properties. | many microorganisms produce enzymes which lyse the walls of yeasts, fungi, and bacteria. the proportions of different enzyme activities present in the lytic system, their action patterns, synergism, and dependence on inhibitors, constitute the activity profile of the lytic system. taken together, the activity profile and process conditions for lysis determine the reaction rate and the distribution of products from lysis of any given type of cells. kinetics of glucan hydrolysis, proteolysis, and ... | 1987 | 18581424 |
| kinetics of enzymatic lysis and disruption of yeast cells: ii. a simple model of lysis kinetics. | a simple two-step model is proposed to describe the kinetics of the two lytic systems examined in the preceding article. the model predicts concentrations of yeast solids, soluble proteins, peptides, and carbohyrates. in the first reaction step, yeast cell mass is solubilized; in the second, the released protein can be hydrolyzed to peptides. kinetics for both yeast lysis and the subsequent protein breakdown are based on michaelis-menten expressions. terms have been included for competitive inhi ... | 1987 | 18581425 |
| molecular characterization of the bacterial communities in the different compartments of a full-scale reverse-osmosis water purification plant. | the origin, structure, and composition of biofilms in various compartments of an industrial full-scale reverse-osmosis (ro) membrane water purification plant were analyzed by molecular biological methods. samples were taken when the ro installation suffered from a substantial pressure drop and decreased production. the bacterial community of the ro membrane biofilm was clearly different from the bacterial community present at other locations in the ro plant, indicating the development of a speci ... | 2008 | 18621875 |
| a bacterial ice-binding protein from the vostok ice core. | bacterial and yeast isolates recovered from a deep antarctic ice core were screened for proteins with ice-binding activity, an indicator of adaptation to icy environments. a bacterial strain recovered from glacial ice at a depth of 3,519 m, just above the accreted ice from subglacial lake vostok, was found to produce a 54 kda ice-binding protein (genbank eu694412) that is similar to ice-binding proteins previously found in sea ice diatoms, a snow mold, and a sea ice bacterium. the protein has th ... | 2008 | 18622572 |
| colonization of overlaying water by bacteria from dry river sediments. | we studied the diversity, community composition and activity of the primary microbial colonizers of the water above freshly re-wetted sediments from a temporary river. dried sediments, collected from mulargia river (sardinia, italy), were covered with sterile freshwater in triplicate microcosms, and changes of the planktonic microbial assemblage were monitored over a 48 h period. during the first 9 h bacterial abundance was low (1.5 x 10(4) cells ml(-1)); it increased to 3.4 x 10(6) cells ml(-1) ... | 2008 | 18643927 |
| identification of three proteins up-regulated by raw starch in cytophaga sp. | raw starch-digesting amylases (rsdas) in many microorganisms convert starch granules into maltodextrins and simple sugars. we cloned and sequenced from cytophaga sp. an rsda with an excellent raw starch digestion activity. this rsda was highly inducible by raw starch, but not by other sugars, suggesting that an unknown signal transduction mechanism is involved in the degradation of raw starch. we used a proteomic approach to investigate the effect of raw starch on protein expression in cytophaga ... | 2008 | 18704367 |
| shifts in microbial and chemical patterns within the marine sponge aplysina aerophoba during a disease outbreak. | the microbial community composition in affected and unaffected portions of diseased sponges and healthy control sponges of aplysina aerophoba was assessed to ascertain the role of microbes in the disease process. sponge secondary metabolites were also examined to assess chemical shifts in response to infection. the microbial profile and aplysinimine levels in unaffected tissue near the lesions closely reflected those of healthy sponge tissue, indicating a highly localized disease process. dgge d ... | 2008 | 18783385 |
| microbial community in the rhizosphere of young maize seedlings is susceptible to the impact of introduced pseudomonads as indicated by fame analysis. | two species of pseudomonas (i.e. p. chlororaphis or p. putida) derived from a maize rhizosphere were studied for their impact on the structure of the microbial community in the rhizosphere of young maize seedlings after inoculation. the culturable bacteria and total microbial communities were analyzed based on profiles of whole-cell fatty acid methyl esters (midi-fame). the introduction of pseudomonas species resulted in the shift from the gram-positive dominated culturable community in the rhiz ... | 2008 | 18802319 |
| physico-chemical and microbial characteristics of the coral reef environment of the gulf of mannar marine biosphere reserve, india. | investigation on physico-chemical parameters and bacteial characteristics of the coral reef environs of the gulf of mannar biosphere reserve was studied. the study found the influence of different physico-chemical parameters on one another and also on the distribution of the total heterotrophic bacteria (thb) in the coral reef areas. nutrients exhibited considerable seasonal and spatial variations with influence on the bacterial population. coral reef areas recorded higher bacterial population d ... | 2008 | 18831378 |
| the commensal microbiology of the gastrointestinal tract. | the gastrointestinal (gi) tract is a dynamic environment and therefore the stability of the commensal community, or microbiota, is under constant challenge. microscopic observations have revealed that the majority of bacteria present in the gi tract are not detected using standard culturing techniques, however with the application of culture-independent techniques it has been estimated that between 500 to 1000 bacterial species inhabit the human gi tract. numerically predominant organisms in the ... | 2008 | 18841700 |
| substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment. | marine microorganisms that consume one-carbon (c(1)) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry. this study investigated marine bacterial communities involved in the metabolism of c(1) compounds. these communities were of relevance to surface seawater and atmospheric chemistry in the context of a bloom that was dominated by phytoplankton known to produce dimethylsulfoniopropionate. in addition to using 16s rrna gen ... | 2008 | 18849453 |
| specific microbiota direct the differentiation of il-17-producing t-helper cells in the mucosa of the small intestine. | the requirements for in vivo steady state differentiation of il-17-producing t-helper (th17) cells, which are potent inflammation effectors, remain obscure. we report that th17 cell differentiation in the lamina propria (lp) of the small intestine requires specific commensal microbiota and is inhibited by treating mice with selective antibiotics. mice from different sources had marked differences in their th17 cell numbers and animals lacking th17 cells acquired them after introduction of bacter ... | 2008 | 18854238 |
| characterization of the bacterial community associated with body wall lesions of tripneustes gratilla (echinoidea) using culture-independent methods. | the bacterial community associated with skin lesions of the sea urchin tripneustes gratilla was investigated using 16s ribosomal rna gene cloning and fluorescent in situ hybridization (fish). all clones were classified in the alphaproteobacteria, gammaproteobacteria and cytophaga-flexibacter-bacteroides (cfb) bacteria. most of the alphaproteobacteria were related to the roseobacter lineage and to bacteria implicated in marine diseases. the majority of the gammaproteobacteria were identified as v ... | 2009 | 19041326 |
| microbial community variation and functions to excess sludge reduction in a novel gravel contact oxidation reactor. | excess biomass produced within the degradation processes of organic pollutants is creating environmental challenges. the gravel contact oxidation reactor (gcor) filled with crushed stone globular aggregates as carriers, has been demonstrated capable of reducing the excess sludge effectively in some pilot and small-scale engineering studies. in order to evaluate the variation and structure of the microbial community and their functions to excess sludge reduction in gcor, a conventional activated ... | 2009 | 19070427 |
| [effects of exotic larix kaempferi on forest soil quality and bacterial diversity]. | the study on the soil quality and bacterial diversity under 8-30 years old exotic larix kaempferi, native pinus tabulaeformis, and secondary deciduous broadleaf forest stands in xiaolong-shan mountains of gansu, northwest china showed that the soil ph under different forest stands had no distinct variation, but soil moisture content was increased with increasing age of forest stands. soil organic matter and nitrogen contents were the highest under secondary deciduous forest, followed by under l. ... | 2008 | 19123342 |
| effect of covering composting piles with mature compost on ammonia emission and microbial community structure of composting process. | to control ammonia (nh(3)) volatilization from the dairy cattle (bos taurus) manure composting process, a compost pile was covered with mature compost and the gas emissions evaluated using the dynamic chamber system. the peak of nh(3) volatilization observed immediately after piling up of the compost was reduced from 196 to 62 mg/m(3) by covering the compost pile with mature compost. the accumulation of nh(4)-n to the covered mature compost was also observed. covering and mixing the compost with ... | 2009 | 19202030 |
| isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities. | this study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell anadara broughtoni inhabiting in the sea of japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. a total of 149 strains were isolated and identified phenotypically. a total of 27 strains were selected to be investigated phylogenetically by 165 rrna gene sequence analysis. the most bacteria were aff ... | 2008 | 19216104 |
| quantitative population dynamics of microbial communities in plankton-fed microbial fuel cells. | this study examines changes in diversity and abundance of bacteria recovered from the anodes of microbial fuel cells (mfcs) in relation to anode potential, power production and geochemistry. mfcs were batch-fed with plankton, and two systems were maintained at different potentials whereas one was at open circuit for 56.8 days. bacterial phylogenetic diversity during peak power was assessed from 16s rdna clone libraries. throughout the experiment, microbial community structure was examined using ... | 2009 | 19242533 |
| ingestion of bacteria overproducing dnak attenuates vibrio infection of artemia franciscana larvae. | feeding of bacterially encapsulated heat shock proteins (hsps) to invertebrates is a novel way to limit vibrio infection. as an example, ingestion of escherichia coli overproducing prokaryotic hsps significantly improves survival of gnotobiotically cultured artemia larvae upon challenge with pathogenic vibrio campbellii. the relationship between hsp accumulation and enhanced resistance to infection may involve dnak, the prokaryotic equivalent to hsp70, a major molecular chaperone in eukaryotic c ... | 2009 | 19373565 |
| [morphologic changes in the life cycle of cytophaga hutchinsonii]. | to study the morphological changes of cytophaga hutchinsonii cell during its life circle. | 2009 | 19388259 |
| [bacterial diversity of disused thermal vents in tibetan yangbajing region]. | to study the bacterial diversity in sediment and water from two disused thermal vents in yangbajing, tibet, china. | 2009 | 19445178 |
| [bacterial diversity in guangxi buffalo rumen]. | to analyze the diversity of bacterial community in guangxi buffalo rumen and to identify the possible cellulolytic bacterial group. | 2009 | 19445183 |
| effects of seawater ozonation on biofilm development in aquaculture tanks. | microbial biofilms developing in aquaculture tanks represent a reservoir for opportunistic bacterial pathogens, and procedures to control formation and bacterial composition of biofilms are important for the development of commercially viable aquaculture industries. this study investigated the effects of seawater ozonation on biofilm development on microscope glass slides placed in small-scale aquaculture tanks containing the live feed organism artemia. fluorescence in situ hybridization (fish) ... | 2009 | 19446976 |
| inorganic phosphorus and nitrogen modify composition and diversity of microbial communities in water of mesotrophic lake. | the effects of inorganic nutrients (n, p) enrichment of mesotrophic lake water on changes in bacterial and protistan (heterotrophic nanoflagellates and ciliates) communities compositions were studied in the mesocosm experiment. phosphorus (po4(3-)) and nitrogen (nh4+) alone and in combination were added to three types of experimental mesocosms. mesocosms results suggested that simultaneous addition of p and n stimulated phytoplankton growth and production rates of bacterial biomass its turnover ... | 2009 | 19469290 |
| extremophile culture collection from andean lakes: extreme pristine environments that host a wide diversity of microorganisms with tolerance to uv radiation. | a total of 88 bacterial strains were isolated from six andean lakes situated at altitudes ranging from 3,400 to 4,600 m above sea level: l. aparejos (4,200 m), l. negra (4,400 m), l. verde (4,460 m), l. azul (4,400 m), l. vilama (4,600 m), and salina grande (3,400 m). salinity ranged from 0.4 to 117 ppm. general diversity was determined by denaturing gradient gel electrophoresis (dgge) analysis. from the excised dgge bands, 182 bacterial sequences of good quality were obtained. gammaproteobacter ... | 2009 | 19495855 |
| microbial diversity of a sulfide black smoker in main endeavour hydrothermal vent field, juan de fuca ridge. | submarine hydrothermal vents are among the least-understood habitats on earth but have been the intense focus of research in the past 30 years. an active hydrothermal sulfide chimney collected from the dudley site in the main endeavour vent field (mef) of juan de fuca ridge was investigated using mineralogical and molecular approaches. mineral analysis indicated that the chimney was composed mainly of fe-, zn-and cu-rich sulfides. according to phylogenetic analysis, within the crenarchaeota, clo ... | 2009 | 19557339 |
| a small population of planktonic flavobacteria with disproportionally high growth during the spring phytoplankton bloom in a prealpine lake. | bacterioplankton growth in temperate lake zurich (switzerland) was studied during the spring phytoplankton bloom by in situ techniques and short-term dilution bioassays. a peak of chlorophyll a (chl a) concentrations was followed by a rise of bacterial cell numbers and leucine assimilation rates, of the proportions of cells incorporating 5-bromo-2-deoxyuridine (brdu), and of community net growth rates in dilution cultures. incorporation of brdu was low in betaproteobacteria (2 +/- 1%), indicatin ... | 2009 | 19601962 |
| marinifilum fragile gen. nov., sp. nov., isolated from tidal flat sediment. | a facultatively anaerobic, moderately halophilic, gram-negative, filamentous bacterium, designated jc2469t, was isolated from tidal flat sediment in korea. phylogenetic analysis based on 16s rrna gene sequences showed that the isolate belonged to the phylum bacteroidetes and its closest taxonomic relative was cytophaga fermentans ncimb 2218t (89.6% sequence similarity). cells appeared as filaments during exponential growth, but became fragmented to rods at stationary phase. spherical cells were ... | 2009 | 19620374 |
| isolation and identification of bacteriophages infecting ayu plecoglossus altivelis altivelis specific flavobacterium psychrophilum. | in order to investigate methods for controlling systemic bacterial coldwater disease (cwd), bacteriophages that infect flavobacterium psychrophilum were isolated by the enrichment method from pond water collected from japanese ayu farms. the five phages isolated were classified as members of myoviridae (pfpw-3, pfpc-y), podoviridae (pfpw-6, pfpw-7), and siphoviridae (pfpw-8) and had highly variable patterns of infectivity for different f. psychrophilum isolates (n=128). the stability tests of th ... | 2010 | 19647377 |
| bacterial diversity in the rumen of gayals (bos frontalis), swamp buffaloes (bubalus bubalis) and holstein cow as revealed by cloned 16s rrna gene sequences. | libraries of rumen bacterial 16s rrna gene sequences of gayals (bos frontalis) and swamp buffaloes (bubalus bubalis) were cloned and sequenced in the present work to compare the bacterial diversity with the third published library of holstein cow. sequence similarity of 97% was used as the definition of operational taxonomic unit (otu). the majority of the 470 sequences retrieved fell into the phyla of low g + c subdivision (329 sequences) and cytophaga-flexibacter-bacteroides (cfb, 123 sequence ... | 2010 | 19662514 |
| cultivation-independent population analysis of bacterial endophytes in three potato varieties based on eubacterial and actinomycetes-specific pcr of 16s rrna genes. | abstract endophytic bacteria are ubiquitous in most plants and colonise plants without exhibiting pathogenicity. studies on the diversity of bacterial endophytes have been mainly approached by characterisation of isolates obtained from internal tissues. despite the broad application of culture-independent techniques for the analysis of microbial communities in a wide range of natural habitats, little information is available on the species diversity of endophytes. in this study, microbial commun ... | 2002 | 19709181 |
| the ecology of cytophaga-flavobacteria in aquatic environments. | | 2002 | 19709188 |
| bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (zea mays) and comparison to its non-transgenic cultivar bosphore. | bacterial communities in rhizospheres of transgenic maize (zea mays, with the pat-gene conferring resistance to the herbicide glufosinate; syn. l-phosphinothricin) were compared to its isogenic, non-transgenic cultivar. total dna was extracted from bacterial cell consortia collected from rhizospheres of plants grown in an agricultural field. with the use of three different primer pairs binding to evolutionarily conserved regions of the bacterial 16s rrna gene, partial sequences were amplified by ... | 2002 | 19709208 |
| molecular characterization of microbial community in nitrate-removing activated sludge. | abstract the microbial community composition and dominant denitrifying populations in high-nitrate-removing (cr-i) and low-nitrate-removing (cr-ii) activated sludge from continuous bioreactors were investigated with most probable number (mpn) enumeration, fluorescence in situ hybridization (fish) and 16s rdna characterization. mpns of nitrate-reducing bacteria of sludge cr-i and sludge cr-ii were 2.82x10(7) and 2.69x10(4) colony-forming units ml(-1), respectively. eight denitrifying bacteria and ... | 2002 | 19709242 |
| genetic diversity of 'satellite' bacteria present in cultures of marine diatoms. | abstract uni-algal, non-axenic cultures of six marine diatoms were screened by polymerase chain reaction-denaturing gradient gel electrophoresis for the diversity of the accompanying bacterial communities ('satellite' bacteria) in order to test the hypothesis that algal cells constitute niches for specific bacterial species. the complexity of the satellite assemblages, as judged from the number of detected phylotypes, was low when compared to the complexity of bacterial assemblages in nature. ge ... | 2002 | 19709263 |
| molecular characterization of bacterial diversity in lodgepole pine (pinus contorta) rhizosphere soils from british columbia forest soils differing in disturbance and geographic source. | rhizosphere bacteria from lodgepole pine (pinus contorta) seedlings were characterized from forest soils which differed in disturbance and geographic source. soil disturbance treatments included whole-tree harvesting with and without heavy soil compaction and whole-tree harvesting with complete surface organic matter removal and heavy soil compaction from british columbia (bc) ministry of forests long-term soil productivity installations in three biogeoclimatic subzones in central bc, canada. ba ... | 2002 | 19709294 |
| phylogenetic characterization of the bacterial assemblage associated with mucous secretions of the hydrothermal vent polychaete paralvinella palmiformis. | as part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous secretions of the northeastern pacific vent polychaete paralvinella palmiformis. using a molecular 16s rdna-based phylogenetic approach, clone libraries were constructed from two samples collected from active sulfide edifices in two hydrothermal vent fields. in bo ... | 2002 | 19709305 |