| role of hsp70 (dnak-dnaj-grpe) and hsp100 (clpa and clpb) chaperones in refolding and increased thermal stability of bacterial luciferases in escherichia coli cells. | the role of chaperones hsp70 (dnak-dnaj-grpe) and hsp100 (clpa-clpb-clpx) in refolding of thermoinactivated luciferase from the marine bacterium photobacterium fischeri and the terrestrial bacterium photorhabdus luminescens has been studied. these luciferases are homologous, but differ greatly in the rate of thermal inactivation and the rate constant for the luminescence reaction. it was shown that refolding of thermoinactivated luciferases is completely determined by the dnak-dnaj-grpe system. ... | 2002 | 12387711 |
| diffusion of beta-lactam antibiotics through oligomeric or monomeric porin channels of some gram-negative bacteria. | the penetration of anionic beta-lactam antibiotics through porins was evaluated as a mechanism of drug resistance. the major proteins with porin activity were purified from the outer membranes of six bacteria. three of the six porins were oligomeric porins. the molecular weights of their monomers were 37 kda from photobacterium damsela, 42 kda from serratia liquefaciens, and 36 kda from e. coli b. the other three porins were heat-modifiable monomeric porins with molecular weights of 43 kda from ... | 2002 | 12402087 |
| hydrogen production by cyanobacteria in an automated outdoor photobioreactor under aerobic conditions. | the possibility of hydrogen production by a hydrogenase impaired mutant strain of anabaena variabilis in outdoor culture was studied. a computer-controlled rooftop (outdoor) tubular photobioreactor (4.35 l) was assembled. h(2) production rates by a. variabilis pk84 grown in co(2) + air in the photobioreactor were measured together with other parameters such as temperature, irradiance, ph, dry biomass weight, and po(2), and chl a concentrations during summer months of 1998 and 1999. efficiencies ... | 2002 | 12402323 |
| seasonality of chesapeake bay bacterioplankton species. | bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ... | 2002 | 12406742 |
| bacteria of the gamma-subclass proteobacteria associated with zooplankton in chesapeake bay. | the seasonal abundance of gamma-subclass proteobacteria, vibrio-photobacterium, vibrio cholerae-vibrio mimicus, vibrio cincinnatiensis, and vibrio vulnificus in the choptank river of chesapeake bay associated with zooplankton was monitored from april to december 1996. large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. larg ... | 2002 | 12406743 |
| [interaction of cationic surface-active antiseptics and serum albumin by the bacterial bioluminescence method]. | human serum albumin binding of cationic surface-active antiseptics was studied using a photobacterium phosphoreum k3 strain isolated by the authors in the black sea. binding of cationic surface-active proteins to serum albumin led to disappearance of toxic and antibacterial characteristics of miramistine, ethonium, and decametoxin, but not chlorohexidine. the activity of chlorohexidine was reduced by 45-48% in the presence of albumin. albumin binding capacity towards miramistine was 7.0-8.7%, to ... | 2002 | 12412506 |
| stimulated biosynthesis of flavins in photobacterium phosphoreum ifo 13896 and the presence of complete rib operons in two species of luminous bacteria. | photobacterium phosphoreum ifo 13896 emits light strongly when cultured in medium containing 3% nacl, but only weakly in medium containing 1% nacl. it is known that dim or dark mutants appear frequently and spontaneously from this parent strain. to confirm that riboflavin biosynthesis is stimulated when the lux operon is active, the amount of light emitted and flavins synthesized under strongly or weakly light emitting conditions was determined. in comparison with the parent strain cultured in 3 ... | 2002 | 12444973 |
| qsar of ecotoxicological data on the basis of data-driven if-then-rules. | a rather small data matrix of seven chemicals and 17 different ecotoxicological end points is examined by methods of discrete mathematics. especially, the lattice theory and its variant, the formal concept analysis may be an attractive tool to analyze quantitative structure activity relationships, when a numerical functional approach is not at hand. the central item is the so called concept, which is a pair of subsets: a subset of molecules and a subset of properties which correspond to each oth ... | 2002 | 12463679 |
| solute accumulation in the deep-sea bacterium photobacterium profundum. | the identity and amounts of intracellular solutes in the deep-sea bacterium photobacterium profundum strain ss9 were studied using nuclear magnetic resonance techniques. p. profundum strain ss9, a moderate piezophile which grows optimally at 20-30 mpa primarily accumulated glutamate and betaine, with lesser amounts of alanine, beta-hydroxybutyrate (beta-hb) and oligomers composed of the beta-hb units when grown at 0.1 mpa to early stationary phase. when grown at the optimal pressure, the cells p ... | 2002 | 12486460 |
| comparative study of four qsar models of aromatic compounds to aquatic organisms. | quantitative structure-activity relationships (qsars) were developed for 43 aromatic compounds toxicity to photobacterium phosphoreum and daphnia magna based on four methods: octanol/water partition coefficient, linear solvation energy relationship, molecular connectivity index and group contribution. through the evaluation of four qsar methods, lser was proved to be the best. and it applied to the widest range of chemicals with the greatest accuracy. | 2002 | 12491732 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| numerical taxonomy of vibrionaceae isolated from cultured amberjack (seriola dumerili) and surrounding water. | a numerical taxonomic study was performed on 148 isolates of gram-negative, heterotrophic, facultative anaerobic bacteria isolated from amberjack (seriola dumerili) and its surrounding culture water. the study included 30 type and reference strains belonging to genera vibrio, listonella, and photobacterium. the strains were characterized by 109 morphological, biochemical, physiological, and nutritional tests. cluster analysis of similarity matrices obtained with s(sm) and s(j) coefficients was c ... | 2003 | 12567241 |
| bioluminescence assays: effects of quinones and phenols. | the influence of a series of quinones and phenols on bacteria bioluminescence systems was investigated. three bioluminescence systems used in ecological monitoring were compared: (1) water-soluble; (2) immobilized in starch gel coupled enzyme systems: nadh:fmn-oxidoreductase-luciferase; (3) luminescent bacteria. bioluminescence inhibition constants of quinones and phenols and bioluminescence induction periods were compared. these kinetic parameters are proportional to quinone concentrations and ... | 2002 | 12568457 |
| active-site copper and zinc ions modulate the quaternary structure of prokaryotic cu,zn superoxide dismutase. | the influence of the constitutive metal ions on the equilibrium properties of dimeric photobacterium leiognathi cu,zn superoxide dismutase has been studied for the wild-type and for two mutant protein forms bearing a negative charge in the amino acid clusters at the dimer association interface. depletion of copper and zinc dissociates the two mutant proteins into monomers, which reassemble toward the dimeric state upon addition of stoichiometric amounts of zinc. pressure-dependent dissociation i ... | 2003 | 12595249 |
| patch-clamp experiments with porins extracted from a marine bacterium (photobacterium profundum strain ss9) and reconstituted in liposomes. | the reconstitution of bacterial porins in liposome bilayers for patch-clamp recording is well established. however, the solutions used in the dehydration, rehydration, and osmotic swelling of the liposomes have been developed for porins from enteric bacteria. porins from marine bacteria normally function in contact with seawater whose ionic composition and osmotic pressure would appear to be incompatible with the established methods. here, we show that, contrary to expectation, an established re ... | 2003 | 12625625 |
| multiple computer-automated structure evaluation study of aquatic toxicity. iii. vibrio fischeri. | an acute toxicity model was constructed on the basis of 901 chemicals tested for toxicity against the luminescent bacteria vibrio fischeri (formerly photobacterium phosphoreum, the microtox test). the model was created using the multiple computer-automated structure evaluation (m-case) program. the model can correctly predict acute toxicity for 92% of the compounds with an error averaging 0.55 log units per median effect concentration (ec50). the main toxicophores, corresponding to polar and non ... | 2003 | 12627631 |
| cloning and nucleotide sequence analysis of the chloramphenicol resistance gene on conjugative r plasmids from the fish pathogen photobacterium damselae subsp. piscicida. | transferable resistance to various drugs was investigated in photobacterium damselae subsp. piscicida from japan. drug resistances were transferred via plasmids of 100, 50, and 40 kb. resistance to chloramphenicol (cmr) was transferred on plasmids of all 3 sizes. the cmr gene (cat) was cloned from the 50 kb plasmids ppdp8511 and ppdp9106 transferred from p. damselae subsp. piscicida strains isolated in different years and places in japan. subcloning localized the cat to within 1.5 kb hindiii-hin ... | 2003 | 12650243 |
| reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring. | the luminous intensity of dark variant (s1) separated from photobacterium phosphoreum (a2) was 1/10,000 less than that of wild-type. ethidium bromide (eb) (0.6 mg/l), mytomycin c (mc, 0.05 mg/l), 2-amino fluorene (2-af, 1.0 mg/l) all could strongly induce reversion mutation for s1 within 24 h and increase reversion ratio significantly. the results of experiments indicated that these revertants had stable genetic characteristic and the mutation may take place at gene levels. the mutagenesis to s1 ... | 2002 | 12658797 |
| use of partition coefficients to predict mixture toxicity. | by using the c(18)-empore disks/water partition coefficient (k(md)) to describe the toxicity of 50 mixed halogenated benzenes to photobacterium phosphoreum, an approach is proposed in this study. application of the approach to the 15 other related mixtures prove the predictive capability of this k(md)-based approach, due to the consistency between the predicted toxicity and the observed ones with r(2)=0.929, se=0.104, f=169.513 at p<0.001. further analysis of this approach finds that, for the mi ... | 2003 | 12691908 |
| interaction of aromatic compounds with photobacterium leiognathi luciferase: fluorescence anisotropy study. | the time-resolved and steady-state fluorescence techniques were employed to elucidate possible interactions of four aromatic compounds (anthracene, popop, msb and 1,4-naphthalendiol) with bacterial luciferase. fluorescence spectra and fluorescence anisotropy decays of these compounds were studied in ethanol, water-ethanol solutions and in the presence of bacterial luciferase. shifts of fluorescent spectra and differences in rotational correlation times are interpreted in terms of weak (hydrophob ... | 2003 | 12701091 |
| the acute toxicity of gluconic acid, beta-alaninediacetic acid, diethylenetriaminepentakismethylenephosphonic acid, and nitrilotriacetic acid determined by daphnia magna, raphidocelis subcapitata, and photobacterium phosphoreum. | acute toxicity of four relatively new chelating agents and their equimolar manganese and cadmium complexes was studied. the chelating agents studied were gluconic acid (ga), beta-alaninediacetic acid (ada), diethylenetriaminepentakismethylenephosphonic acid (dtpmp), and nitrilotriacetic acid (nta). three common bioassays, namely daphnia magna, raphidocelis subcapitata, and photobacterium phosphoreum (microtox bioassay) were applied. r. subcapitata proved the most sensitive to these compounds. wi ... | 2003 | 12712292 |
| pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream sparus aurata. | the present study investigated the kinetic profile of flumequine (flu) in gilthead sea bream sparus aurata (170 g) held at 19 degrees c and evaluated its in vitro efficacy against important bacterial diseases in mediterranean mariculture. following a single intravascular injection (10 mg kg(-1) fish), the distribution half-life (t1/2alpha) and the half-life of the terminal phase of elimination (t1/2gamma) of the drug were 0.2 and 30 h respectively. tissue penetration of flu was low, since both t ... | 2003 | 12718468 |
| standardized system for quantifying residual dirt on medical appliances cleansed in hospital washers--disinfectors: dirt detection by different methods. | an easy-to construct, easy-to-operate standardized system was developed for determining the residual biological contamination of surgical instruments, endoscopes and other medical appliances subjected to hospital cleansing and/or disinfection. it consists of standard-sized pieces of glass, metal or endoscope plastic--dirt carriers--either bare or enclosed in truncated eppendorf caps to simulate hard-to-access conditions. the surface of the carriers is covered with model dirt simulating biologica ... | 2003 | 12744084 |
| carriage of potentially fish-pathogenic bacteria in sparus aurata cultured in mediterranean fish farms. | a bacteriological survey of gilthead sea bream sparus aurata from different fish farms and culture systems on the spanish mediterranean coast was conducted. three different studies were performed. study a included hatchery-reared larvae; study b, periodic examination of randomly sampled growing fish; and study c, growing fish sampled only during mortality/morbidity events. in studies b and c, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical sign ... | 2003 | 12747637 |
| acute and chronic toxicity of dimethylsulfoxide to daphnia magna. | | 2003 | 12756470 |
| static and dynamic water molecules in cu,zn superoxide dismutase. | understanding protein hydration is a crucial, and often underestimated issue, in unraveling protein function. molecular dynamics (md) computer simulation can provide a microscopic description of the water behavior. we have applied such a simulative approach to dimeric photobacterium leiognathi cu,zn superoxide dismutase, comparing the water molecule sites determined using 1.0 ns md simulation with those detected by x-ray crystallography. of the water molecules detected by the two techniques, 20% ... | 2003 | 12784219 |
| biodegradation of plasticizers by rhodotorula rubra. | the degradation of plasticizers by the yeast rhodotorula rubra j-96-1 (american type culture collection 9449) in the presence of glucose was studied. the plasticizers included the commonly used bis-2-ethylhexyl adipate (b(eh)a), dioctyl phthalate (dop), and dioctyl terephthalate (dotp), and the less commonly used dipropylene glycol dibenzoate (d(pg)db) and diethylene glycol dibenzoate (d(eg)db). the proposal had been made that the latter two plasticizers be used as alternatives to the first thre ... | 2003 | 12785580 |
| [the inhibitory effect of extracts from galla chinensis on marine fouling bacteria]. | to observe the activity of extracts from galla chinensis with different solvents against marine fouling bacteria in vitro. | 2003 | 12795221 |
| quantification of joint effect for hydrogen bond and development of qsars for predicting mixture toxicity. | a qsar model is successfully proposed to predict the toxicity effect on photobacterium phosphoreum by nonpolar-narcotic-chemical mixtures and/or polar-narcotic-chemical mixtures. for nonpolar-narcotic-chemical mixtures and polar-narcotic-chemical mixtures, their corresponding hydrophobicity-based qsar models are derived from regression analysis. comparison of these two qsar models make us believe that it is the joint effect of hydrogen bond in polar-narcotic-chemical mixture that leads to the di ... | 2003 | 12821001 |
| apoptosis of sea bass (dicentrarchus labrax l.) neutrophils and macrophages induced by experimental infection with photobacterium damselae subsp. piscicida. | the infection of sea bass (dicentrarchus labrax l.) by intraperitoneal (i.p.) injection of the agent of fish pasteurellosis photobacterium damselae subsp. piscicida resulted in the apoptosis of peritoneal neutrophils and macrophages. all the eight virulent and none of the two non-virulent strains tested exhibited apoptogenic activity. a secreted bacterial protein(s) is a likely candidate as the factor(s) responsible for this activity, since no apoptosis was induced by i.p. injected uv-killed vir ... | 2003 | 12834617 |
| [detection of heterotrophic bacteria and vibrios in shoal of bullacta exarata]. | the number of heterotrophic bacteria and vibrios in shoal for bullacta exarata rearing fluctuated mainly within 1.25 x 10(4)-7.21 x 10(4) cfu.g-1 and 0-1.08 x 10(4) cfu.g-1, respectively. 173 strains of bacteria, mainly belong to 13 genera were isolated. the predominant genera were composed of bacillus, photobacterium, pseudomonas, vibrio and some genera of enterobacteriaceae. the number of bacteria changed in line with the alteration of the temperature, and was especially affected by the organi ... | 2003 | 12836568 |
| dominance of vibrio fischeri in secreted mucus outside the light organ of euprymna scolopes: the first site of symbiont specificity. | previous studies of the euprymna scolopes-vibrio fischeri symbiosis have demonstrated that, during colonization, the hatchling host secretes mucus in which gram-negative environmental bacteria amass in dense aggregations outside the sites of infection. in this study, experiments with green fluorescent protein-labeled symbiotic and nonsymbiotic species of gram-negative bacteria were used to characterize the behavior of cells in the aggregates. when hatchling animals were exposed to 10(3) to 10(6) ... | 2003 | 12839763 |
| toxicity of nitric oxide and peroxynitrite to photobacterium damselae subsp. piscicida. | virulent strains of photobacterium damselae subsp. piscicida (pdp) were grown in media with or without glucose supplementation (to enhance polysaccharide capsule formation) and the bactericidal action of nitric oxide (no) and peroxynitrites was evaluated in a cell-free assay. treatment with the no-donor s-nitroso-acetyl-penicillamine (snap) induced a dose-and time-dependent decrease in pdp survival. this effect was greater for strains grown without glucose supplementation (c forms) than for thei ... | 2003 | 12892745 |
| [study of the binding between bacterial lipopolysaccharides and polymyxin by a bioluminescent method]. | for rating the interaction of lipopolysaccharides (lps) with polymixin b (pmb) a bacterial bioluminescence is offered to be used. bioluminescence level of bacteria decreases under free antibiotic amounts action. it is shown, that as a result of interaction with lps antibiotic properties of pmb are reduced, and the intensity of bacterial bioluminescence is restored. the bioluminescence level in such system characterizes the lps quantity. kinetic properties of bacterial light emission at the prese ... | 2002 | 12924022 |
| concerted simulations reveal how peroxidase compound iii formation results in cellular oscillations. | a major problem in mathematical modeling of the dynamics of complex biological systems is the frequent lack of knowledge of kinetic parameters. here, we apply brownian dynamics simulations, based on protein three-dimensional structures, to estimate a previously undetermined kinetic parameter, which is then used in biochemical network simulations. the peroxidase-oxidase reaction involves many elementary steps and displays oscillatory dynamics important for immune response. brownian dynamics simul ... | 2003 | 12944259 |
| similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates. | tetracycline-resistant (tet(r)) bacteria were isolated from fishes collected at three different fish farms in the southern part of japan in august and september 2000. of the 66 tet(r) gram-negative strains, 29 were identified as carrying tetb only. four carried tety, and another four carried tetd. three strains carried tetc, two strains carried tetb and tety, and one strain carried tetc and tetg. sequence analyses indicated the identity in tet(r) genes between the fish farm bacteria and clinical ... | 2003 | 12957921 |
| investigation of media formulations promoting differential antigen expression by photobacterium damsela ssp. piscicida and recognition by sea bass, dicentrarchus labrax (l.), immune sera. | photobacterium damsela ssp. piscicida (phdp) isolates were grown in various bacteriological media, in eukaryotic cell culture media and in the presence of fish cells (resembling some aspects of in vivo growth environments). bacterial cells, extracellular products (ecps) and crude capsular polysaccharide were isolated and analysed by electrophoresis and western blot using sea bass sera. growth in bacteriological media conserved the synthesis of cell and extracellular components when these were co ... | 2003 | 12962207 |
| vaccination trials of sea bass, dicentrarchus labrax (l.), against photobacterium damsela subsp. piscicida, using novel vaccine mixtures. | bacterial cells of the marine fish pathogen photobacterium damsela subsp. piscicida were grown in novel culture media. a mixture of whole cells and extracellular components was inactivated and used in bath, intraperitoneal (i.p.) and oral vaccination of sea bass, dicentrarchus labrax, employing two sizes of fish. a commercial vaccine was used for comparative purposes. control and immunized fish were either bath or intraperitoneally challenged 6 and 12 weeks post-vaccination. small fish had signi ... | 2003 | 12962216 |
| survival against exogenous hydrogen peroxide of photobacterium damselae subsp. piscicida under different culture conditions. | | 2003 | 12962239 |
| the sos-lux-lac-fluoro-toxicity-test on the international space station (iss). | in the 21st century, an increasing number of astronauts will visit the international space station (iss) for prolonged times. therefore it is of utmost importance to provide necessary basic knowledge concerning risks to their health and their ability to work on the station and during extravehicular activities (eva) in free space. it is the aim of one experiment of the german project triple-lux (to be flown on the iss) to provide an estimation of health risk resulting from exposure of the astrona ... | 2003 | 12971406 |
| the decarboxylation of amino acids by photobacterium fischeri. | | 1953 | 13034892 |
| the taxonomy of certain luminous bacteria. | | 1955 | 13252219 |
| correlation between luminescence and l-bodies in a strain of photobacterium fischeri. | | 1957 | 13400119 |
| the interaction of luciferase, flavin mono-nucleotide and longchain aldehydes in the light reaction catalyzed by preparations of luminous bacteria. | | 1958 | 13535692 |
| effect of antibodies on the luminescence of achromobacter fischeri as a criterion of cellular penetration. | | 1958 | 13575820 |
| the carboxyl activation of glycine in extracts of photobacterium fischeri. | | 1958 | 13584406 |
| mechanism of the carboxyl activation of glycine in extracts of photobacterium fischeri. | | 1959 | 13651216 |
| some optical properties of luminous bacteria. | | 1959 | 13835177 |
| on the role of long-chain aldehydes in the light reaction in photobacterium phosphoreum enzyme preparations. | | 1960 | 13837482 |
| irradiation of bacterial luciferase in vivo. | | 1962 | 13874983 |
| ion sensitivity of the light reaction catalyzed by enzyme preparations from photobacterium phosphoreum. | | 1961 | 13920343 |
| evidence for the presence of an unknown factor, active in the light reaction, in preparations of photobacterium phosphoreum. | | 1962 | 13920344 |
| [physico-chemical studies on intermediary metabolism of photogenic bacteria]. | | 1962 | 14035710 |
| molybdenum and iron requirements for nitrate reductase in photobacterium sepia. | | 1963 | 14098463 |
| investigations on the identity of the light-emitting molecule in photobacterium phosphoreum. | | 1963 | 14104945 |
| effects of cultural conditions on nitrate reductase in photobacterium sepia. | | 1964 | 14188767 |
| [chemistry of photosynthetic phosphorylization]. | | 1964 | 14218283 |
| [luminescence and oxidative enzyme activity of luminescent bacteria]. | | 1964 | 14237039 |
| [on the action of aminazin on bioluminescent bacteria]. | | 1964 | 14238144 |
| on the molecular mechanism of bioluminescence. i. the role of long-chain aldehyde. | | 1964 | 14243528 |
| influence of some inhibiting and activating substances on the light reaction in vitro of photobacterium phosphoreum. | | 1964 | 14249835 |
| on the molecular mechanism of bioluminescence, ii. light-induced luminescence. | | 1965 | 14283197 |
| the purification properties, and chemiluminescent quantum yield of bacterial luciferase. | | 1965 | 14284763 |
| crystalline bacterial luciferase from photoberterium fischeri. | | 1965 | 14324541 |
| [studies on cultivation of photobacterium phosphoreum. 1. factors influencing the growth and light production of photobacterium phosphoreum]. | | 1961 | 14458104 |
| [studies on cultivation of photobacterium phosphoreum. ii. on a large scale cultivation of photobacterium phosphoreum]. | | 1961 | 14458105 |
| [studies on cultivation of photobacterium phosphoreum. iii. on a measurement of light intensity and some applications]. | | 1962 | 14458106 |
| three-dimensional quantitative structure-activity relationship study for phenylsulfonyl carboxylates using comfa and comsia. | from both the comparative molecular field analysis (comfa) and the comparative molecular similarity indices analysis (comsia), the paper describes two three-dimensional quantitative structure-activity relationship (3d-qsar) models for the acute toxicity logec50 (15 min-ec50 in micromoll(-1)) of 56 phenylsulfonyl carboxylates on photobacterium phosphoreum. two models yield the leave-one-out cross-validated correlation coefficient q2 values of 0.823 and 0.713, and the conventional correlation coef ... | 2003 | 14505717 |
| purification and characterization of an antibacterial protein in the skin secretion of rockfish sebastes schlegeli. | an antibacterial protein in the skin secretion of rockfish (sebastes schlegeli) was purified by lectin affinity chromatography on con a-sepharose and gel filtration on tskgel g3000sw. the antibacterial protein featured the high molecular mass and selective action against gram-negative bacteria. the molecular mass of the protein was estimated to be approximately 150 kda in gel filtration and approximately 75 kda by sds-page, suggesting that it is dimeric. the antibacterial principle was an acidic ... | 2003 | 14522599 |
| [comparative study of microbial communities from cultured and natural population of the mussel mytilus trossulus in peter the great bay]. | the 525 strains of heterotrophic bacteria isolated from natural and cultured populations of the mussel mytilus trossulus and the surrounding seawater were identified to a genus level on the basis of phenotypic analysis and the fatty acid composition of cell lipids. gram-negative isolates were dominated by six genera of the family enterobacteriaceae and by the genera pseudoalteromonas, vibrio, photobacterium, cytophaga/flavobacterium/bacteroides, pseudomonas, and moraxella, gram-positive isolates ... | 2003 | 14526545 |
| development of qsars for predicting the joint effects between cyanogenic toxicants and aldehydes. | quantitative structure-activity relationship (qsar) approaches are proposed in this study to predict the joint effects of mixture toxicity. the initial investigation studies the joint effects between cyanogenic toxicants and aldehydes to photobacterium phosphoreum. joint effects are found to result from the formation of a carbanion intermediate produced through the chemical interactions between cyanogenic toxicants and aldehydes. further research indicates that the formation of carbanion interme ... | 2003 | 14565777 |
| [effect of mycotoxin t-2 on bioluminescence intensity of bacteria]. | the acute and chronic toxicity of t-2 was studied by bioluminescent method with the use of two strains of luminous bacteria--p. phosphorum sq3 u v. fischeri f1 as biological objects. it was shown that in acute experiments after 10 min incubation of bacteria in the presence of t-2 the bioluminescence inhibition on the 50% level was observed at the toxin concentration equal to 12 mg/ml. in chronic experiments such a level of bioluminescence inhibition was registered after 16 hours incubation at th ... | 2003 | 14577159 |
| bacterial influences on atlantic halibut hippoglossus hippoglossus yolk-sac larval survival and start-feed response. | a bacteria-free halibut larval rearing system was used to test 20 bacterial isolates, from british halibut hatcheries, for their toxicity towards halibut yolk-sac larvae under microbially controlled conditions. the isolates tested spanned a range of genera and species (pseudoalteromonas, halomonas marina, vibrio salmonicida-like, photobacterium phosphoreum and v. splendidus species). a pathogen of turbot, scophthalmus maximus, v. anguillarum 91079, and 2 isolates from adult halibut were also inc ... | 2003 | 14598986 |
| isolation and identification of photobacterium phosphoreum from an unexpected niche: migrating salmon. | six luminous bacteria were isolated from migrating salmon in the yukon river, alaska. all isolates were identified as photobacterium phosphoreum. previous studies suggest that p. phosphoreum is an exclusively marine bacterium, while our alaskan isolates are from salmon which migrated up to 1,228 km from the marine environment. | 2003 | 14602659 |
| virulence of photobacterium damselae subsp. piscicida in cultured cobia rachycentron canadum. | an outbreak of serious mortality among the cultured cobia rachycentron canadum (weighing 3 kg) characterized by the presence of whitish granulomatous deposits on the kidney, liver and spleen occurred in july of 2000 in taiwan. a non-motile strain cp1 was isolated from kidney and/or liver on tryptic soy agar and/or brain heart infusion agar plates (both supplemented with 1% nacl, w/v). this strain was characterized and identified as photobacterium damselae subsp. piscicida using biochemical chara ... | 2003 | 14625900 |
| nutritional composition and microflora of the fresh and fermented skate (raja kenojei) skins. | the proximate compositions of fresh and fermented skate skin were each 75.95% and 74.5% moisture, 22.7% and 21.8% protein, 0.5% and 0.7% lipid and 0.6% and 0.9% ash, respectively. the predominant minerals were potassium and phosphorus (i.e. 53.5 and 33.0 mg/100 g in fresh skin, and 10.46 and 10.51 mg/100 g in fermented skin, respectively). amino acid concentrations were lower in the fermented skin compared with the fresh skin. histidine, glycine, alanine and glutamic acid were the major free ami ... | 2004 | 14630591 |
| oxidative stress monitoring in biological samples by bioluminescent method. | the integral bioluminescent biotest with lyophilized fluorescent bacteria was used for monitoring of lpo processes in tissue extracts and serum of rats exposed to stress. a relationship between the content of mda (lpo indicator) and fluorescence of bacteria was observed in all biological samples. | 2003 | 14631513 |
| simple and rapid detection of photobacterium damselae ssp. piscicida by a pcr technique and plating method. | to detect photobacterium damselae ssp. piscicida using the pcr technique and plating method. | 2003 | 14633013 |
| accuracy of six commercially available systems for identification of members of the family vibrionaceae. | six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ... | 2003 | 14662957 |
| immune response of gilt-head seabream (sparus aurata) to antigens from photobacterium damselae subsp. piscicida. | | 2004 | 14675834 |
| mechanism of concentration addition toxicity: they are different for nonpolar narcotic chemicals, polar narcotic chemicals and reactive chemicals. | according to the toxicity mechanism of the individual chemicals, the concentration addition toxicity mechanism is revealed for nonpolar-narcotic-chemical mixtures, polar-narcotic-chemical mixtures and reactive-chemical mixtures, respectively. for nonpolar-narcotic-chemical mixtures, the partitioning of individual chemicals from water to biophase was determined, and the result shows that their concentration additive effect results from no competitive partitioning among individual chemicals. for p ... | 2004 | 14675847 |
| modelling and predicting the simultaneous growth of listeria monocytogenes and spoilage micro-organisms in cold-smoked salmon. | to evaluate and model the simultaneous growth of listeria monocytogenes and spoilage micro-organisms in cold-smoked salmon. | 2004 | 14678163 |
| rapidly advancing necrotizing fasciitis caused by photobacterium (vibrio) damsela: a hyperaggressive variant. | to describe the first case of vibrio damsela necrotizing fasciitis in new england, emphasizing the importance of very early operative intervention to achieve source control in this extremely aggressive infection. | 2004 | 14707592 |
| [antimicrobial activity of saponins from hedera taurica c a r r]. | the antibacterial and antifungal action of saponins sx1, taurozide h2, taurozide i and their effect on bioluminescent system of photobacteria photobacterium phosphoreum (cohn) ford were investigated. saponins h2 and i had no antimicrobial effect. saponin sx1 possessed antifungal activity in vitro with respect to candida albicans, c. krusei, c. tropicalis. saponin sx1 is less toxic to p. phosphoreum than well-known antiseptics--chlorhexedin and miramistin. | 2003 | 14723156 |
| construction of a safe, stable, efficacious vaccine against photobacterium damselae ssp. piscicida. | vaccination with bacterial auxotrophs, particularly those with an interruption in the common pathway of aromatic amino-acid biosynthesis, known as the shikimate pathway, has been shown to be effective in the prevention of a variety of bacterial diseases. in order to evaluate this approach to vaccine development in the important marine pathogen photobacterium damselae subsp. piscicida, the aroa gene of the shikimate pathway was identified from a p. damselae subsp. piscicida genomic library by com ... | 2003 | 14735921 |
| evolution of toxicity upon wet catalytic oxidation of phenol. | this work reports on the evolution of the toxicity of phenol-containing simulated wastewater upon catalytic wet oxidation with a commercial copper-based catalyst (engelhard cu-0203t). the results of the study show that this catalyst enhances detoxification, in addition to its effect on the oxidation rate. the ec50 values of the intermediates identified throughout the oxidation route of phenol have been determined and used to predict the evolution of toxicity upon oxidation. the predicted values ... | 2004 | 14740728 |
| interactions of soil-derived dissolved organic matter with phenol in peroxidase-catalyzed oxidative coupling reactions. | the influence of dissolved soil organic matter (dsom) derived from three geosorbents of different chemical composition and diagenetic history on the horseradish peroxidase (hrp) catalyzed oxidative coupling reactions of phenol was investigated. phenol conversion and precipitate-product formation were measured, respectively, by hplc and radiolabeled species analysis. fourier transform infrared (ftir) spectroscopy and capillary electrophoresis (ce) were used to characterize the products of enzymat ... | 2004 | 14740756 |
| collaborative effects of photobacterium cuzn superoxide dismutase (sods) and human ap endonuclease in dna repair and sod-deficient escherichia coli under oxidative stress. | the defenses against free radical damage include specialized repair enzymes that correct oxidative damage in dna and detoxification systems such as superoxide dismutases (sods). these defenses may be coordinated genetically as global responses. we hypothesized that the expression of sod and dna repair genes would inhibit dna damage under oxidative stress. therefore, protection of escherichia coli mutants deficient in sod and dna repair genes (sod-, xth-, and nfo-) was demonstrated by transformin ... | 2004 | 14744629 |
| genomic polymorphism in symbiotic populations of photobacterium leiognathi. | photobacterium leiognathi forms a bioluminescent symbiosis with leiognathid fishes, colonizing the internal light organ of the fish and providing its host with light used in bioluminescence displays. strains symbiotic with different species of the fish exhibit substantial phenotypic differences in symbiosis and in culture, including differences in 2-d page protein patterns and profiles of indigenous plasmids. to determine if such differences might reflect a genetically based symbiont-strain/host ... | 2004 | 14756879 |
| the influence of penicillin on large body production by luminous bacteria. | | 1950 | 14774347 |
| hoarding behavior in the golden hamster (mesocricetus auratus auratus). | | 1950 | 14803683 |
| characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern pacific ocean based on 16s ribosomal dna analysis. | seventy-eight 4 degrees c-culturable bacteria were isolated using zobell 2216e medium from surface (0-200 m) and deep-sea (1000-9671 m) waters in the northwestern pacific ocean. growth studies indicated that all 4 degrees c-culturable bacteria were psychrotrophs. six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. phylogenetic characterization based on 16s ribosomal dna sequence analysis of the representative phylotypes revealed that some bacterial ... | 2001 | 14961338 |
| the effect of in vivo growth on the cellular and extracellular components of the marine bacterial pathogen photobacterium damsela subsp. piscicida. | photobacterium damsela subsp. piscicida, the causative agent of fish pasteurellosis, was grown in vivo. bacterial cells and extracellular products (ecps) were analysed via electrophoresis and immunoblot analysis, using specific sea bass antisera. growth in vivo induced the synthesis of unique bacterial cell proteins at > 206, 206, 21.3, 18, 7.6 and < 7.6 kda. sea bass serum raised against live bacterial cells of the pathogen and especially a sea bass serum raised against formalin-inactivated bac ... | 2004 | 14986934 |
| two cases of fatal necrotizing fasciitis caused by photobacterium damsela in japan. | we encountered two cases of fatal necrotizing fasciitis caused by photobacterium damsela in japan. both cases occurred in fishermen who became sick after fishing. they developed multiple organ failure within 20 to 36 h from the onset of initial symptoms despite intensive chemotherapy and surgical treatments. | 2004 | 15004123 |
| development of a fragment constant method for estimating the mixture toxicity. | based on group contribution theory, a fragment constant model to estimate mixture toxicity is proposed in this paper. the toxicity (ec50m) of 58 mixtures is determined using photobacterium phosphoreum. analysis of these ec50m and the mole fraction of the individual chemical fragments (br, cl, no2, oh, nh2) helps work out the fragment toxicity contribution (deltati) to ec50m. thus, a linear regression equation is established between the toxicity contribution deltati and the fragment constants of ... | 2004 | 15025158 |
| photobacterium phosphoreum caused a histamine fish poisoning incident. | an incident of histamine fish poisoning (hfp) occurred due to the consumption of iwashi maruboshi (dried sardine) in osaka, japan in march 2002. a histamine-producing bacterial strain, ys4-7, was isolated from iwashi maruboshi that contained 1700 mg of histamine per kilogram. this strain was identified as photobacterium phosphoreum by biochemical examinations and partial sequencing of 16s rdna. p. phosphoreum ys4-7 showed greater capability as a histamine producer at 4 and 12 degrees c than morg ... | 2004 | 15033270 |
| phylogenetic analysis of the lux operon distinguishes two evolutionarily distinct clades of photobacterium leiognathi. | the luminous marine bacterium photobacterium mandapamensis was synonymized several years ago with photobacterium leiognathi based on a high degree of phenotypic and genetic similarity. to test the possibility that p. leiognathi as now formulated, however, actually contains two distinct bacterial groups reflecting the earlier identification of p. mandapamensis and p. leiognathi as separate species, we compared p. leiognathi strains isolated from light-organ symbiosis with leiognathid fishes (i.e. ... | 2004 | 15034641 |
| cloning and characterization of the gene encoding for omp-pd porin: the major photobacterium damsela outer membrane protein. | the outer membrane protein of photobacterium damsela (omp-pd) and the gene encoding for this porin protein were isolated and characterized. the deduced amino acid sequence of the omp-pd monomer has 338 amino acids and a calculated molecular weight of 36,951 da. this sequence includes a 22-amino acid signal peptide at the n-terminal, which is not found when the monomer is located in the outer membrane. native omp-pd protein forms a trimeric structure of approximately 110 kda. it exhibits resistan ... | 2004 | 15057460 |
| simultaneous detection of marine fish pathogens by using multiplex pcr and a dna microarray. | we coupled multiplex pcr and a dna microarray to construct an assay suitable for the simultaneous detection of five important marine fish pathogens (vibrio vulnificus, listonella anguillarum, photobacterium damselae subsp. damselae, aeromonas salmonicida subsp. salmonicida, and vibrio parahaemolyticus). the array was composed of nine short oligonucleotide probes (25-mer) complementary to seven chromosomal loci (cyt, rpon, gyrb, toxr, urec, dly, and vapa) and two plasmid-borne loci (fata and a.sa ... | 2004 | 15070982 |
| biosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria. | the biochemistry of piezophilic bacteria is unique in that piezophiles produce polyunsaturated fatty acids (pufas). a pertinent question is if piezophilic bacteria synthesize pufa de novo, through dietary uptake, or both. this study was undertaken to examine the biosynthesis and cellular uptake of pufas by piezophilic bacteria. a moderately piezophilic (shewanella violacea dss12) and two hyperpiezophilic bacteria (s. benthica db21mt-2 and moritella yayanosii db21mt-5) were grown under 50 mpa (me ... | 2004 | 15081997 |