| gastric malt lymphomas are divided into three groups based on responsiveness to helicobacter pylori eradication and detection of api2-malt1 fusion. | gastric malt lymphoma shows unique features including regression by helicobacter pylori eradication and api2-malt1 fusion. we performed a molecular and clinicopathologic study for 115 cases. all eradication-responsive cases were devoid of api2-malt1 fusion. all tumors positive for the fusion and all negative for h. pylori infection were nonresponsive to the eradication. consequently, gastric malt lymphomas were divided into three groups: eradication-responsive and fusion-negative (group a, n = 7 ... | 2004 | 15577674 |
| novel 180- and 480-base-pair insertions in african and african-american strains of helicobacter pylori. | helicobacter pylori is a genetically diverse bacterial species that chronically infects human stomachs and sometimes causes severe gastroduodenal disease. studies of polymorphic dna sequences can suggest geographic origins of individual strains. here, we describe a 180-bp insertion (ins180), which is just after the translation stop of a gene of unknown function, near the promoter of jhp0152-jhp0151 two-component signal transduction genes in strain j99, and absent from this site in strain 26695. ... | 2004 | 15583296 |
| the selective cyclooxygenase-2 inhibitor nimesulide prevents helicobacter pylori-associated gastric cancer development in a mouse model. | helicobacter pylori infection can lead to gastric cancer, and cyclooxygenase-2 (cox-2) is overexpressed in the stomach during h. pylori infection. therefore, we investigated whether nonsteroidal anti-inflammatory drugs might protect against this form of cancer. specifically, we examined the chemopreventive effect of the cox-2 inhibitor nimesulide on h. pylori-associated gastric carcinogenesis in mice. | 2004 | 15585646 |
| induction of peripheral lymph node addressin in human gastric mucosa infected by helicobacter pylori. | helicobacter pylori infects over half the world's population and is a leading cause of peptic ulcer and gastric cancer. h. pylori infection results in chronic inflammation of the gastric mucosa, and progression of chronic inflammation leads to glandular atrophy and intestinal metaplasia. however, how this chronic inflammation is induced or maintained is not well known. here, we show that chronic inflammation caused by h. pylori infection is highly correlated with de novo synthesis of peripheral ... | 2004 | 15591109 |
| human peripheral and gastric lymphocyte responses to helicobacter pylori napa and aphc differ in infected and uninfected individuals. | in this study, we identify the nature of the immunological response of human peripheral blood mononuclear cells (pbmc) and lamina propria gastric lymphocytes (lpl) to two helicobacter pylori antigens, the neutrophil activating protein (napa) and alkyl hydroperoxide reductase (aphc). these antigens were identified and selected for study based on the observation that serological recognition of these proteins was associated with h pylori negative status in humans. | 2005 | 15591500 |
| the interaction of h. pylori infection and nsaids in cyclooxygenase-2 mrna expression in gastric antral, corpus mucosa, and gastric ulcer. | although helicobacter pylori infection and use of nonsteroidal anti-inflammatory drugs (nsaids) are the two major causes of gastric ulcer, their interaction remains controversial. we constructed a prospective cohort study to evaluate how these two factors influence the expression of cox-2 mrna in gastric antral, corpus mucosa, and gastric ulcer. | 2005 | 15599211 |
| nutrients released by gastric epithelial cells enhance helicobacter pylori growth. | helicobacter pylori survives and proliferates in the human gastric mucosa. in this niche, h. pylori adheres to the gastric epithelial cells near the tight junctions. in vitro, h. pylori proliferated well in tissue-culture medium near gastric epithelial cells. however, in the absence of epithelial cells, growth of h. pylori could only be established in tissue-culture medium when, prior to the experiment, it was preincubated near gastric epithelial cells. therefore, we aimed to determine whether d ... | 2004 | 15610074 |
| protective mechanism of epigallocatechin-3-gallate against helicobacter pylori-induced gastric epithelial cytotoxicity via the blockage of tlr-4 signaling. | helicobacter pylori infection leads to gastric mucosal damage by several mechanisms including the direct effect of virulence factors produced by h. pylori, propagation of inflammation, oxidative stress, dna damage, and induction of apoptosis. (-)-epigallocatechin-3-gallate (egcg), one of the green tea catechins, is known to suppress h. pylori-induced gastritis through its antioxidative and antibacterial actions. in this study, we evaluated the protective mechanism of egcg against h. pylori-induc ... | 2004 | 15610077 |
| increased risk of intestinal type of gastric adenocarcinoma in japanese women associated with long forms of ccttt pentanucleotide repeat in the inducible nitric oxide synthase promoter. | tandem repeat number polymorphism of a ccttt pentanucleotide in the promoter region of the inducible nitric oxide synthase gene (inos) and a polymorphism of the interleukin-1beta (il-1b) promoter at position -31 were analyzed in dna samples from 181 japanese control subjects and 158 gastric cancer patients, including 96 intestinal type and 62 diffuse type. an association between the intestinal type of gastric adenocarcinoma and higher promoter activity of the inos gene was found in women, especi ... | 2005 | 15617837 |
| mucosal foxp3-expressing cd4+ cd25high regulatory t cells in helicobacter pylori-infected patients. | helicobacter pylori chronically colonizes the stomach and duodenum and causes peptic ulcers or gastric adenocarcinoma in 10 to 20% of infected individuals. we hypothesize that the inability of patients to clear h. pylori infections is a consequence of active suppression of the immune response. here we show that h. pylori-infected individuals have increased frequencies of cd4(+) cd25(high) t cells in both the stomach and duodenal mucosa compared to uninfected controls. these cells have the phenot ... | 2005 | 15618192 |
| detection of cytomegalovirus, helicobacter pylori and chlamydia pneumoniae dna in carotid atherosclerotic plaques by the polymerase chain reaction. | cytomegalovirus (cmv), helicobacter pylori (h. pylori) and chlamydia pneumoniae (c. pneumoniae) have been associated with human atherosclerosis. the reported rates of detection within atherosclerotic lesions by pcr vary widely for all of these pathogens. we investigated their presence in carotid atherosclerotic plaques. | 2004 | 15636450 |
| matrix metalloproteinase secretion by gastric epithelial cells is regulated by e prostaglandins and mapks. | because matrix metalloproteinases (mmps) play roles in inflammatory tissue injury, we asked whether mmp secretion by gastric epithelial cells may contribute to gastric injury in response to signals involved in helicobacter pylori-induced inflammation and/or cyclooxygenase inhibition. tumor necrosis factor (tnf)-alpha, interleukin (il)-1beta, and epidermal growth factor (egf) stimulated gastric cell mmp-1 secretion, indicating that mmp-1 secretion occurs in inflammatory as well as non-inflammator ... | 2005 | 15640153 |
| the roles of cytokines, inflammation and immunity in vascular diseases. | recent findings regarding the roles of cytokines, inflammation and immunity during the development of atherosclerosis were reviewed. especially, the relationships among pro-inflammatory cytokines such as interleukin (il)-1, il-18 and osteopontin, and anti-inflammatory cytokines such as il-1 receptor antagonist, il-10 and il-18 binding protein to inflammation and atherosclerosis were investigated and are described in detail. in addition, helicobacter pylori and c pneumoniae infections to inflamma ... | 2004 | 15644584 |
| hla polymorphisms are associated with helicobacter pylori infected gastric cancer in a high risk population, china. | helicobacter pylori is one of the most common bacterial infections associated with an increased risk of gastric cancer, but its association with host factors, particularly polymorphisms of the immune response genes, such as human leukocyte antigen (hla) genes, is still unclear. to investigate the role of hla polymorphisms in the risk of gastric cancer among subjects with h. pylori infection, a case-control study involving 52 gastric cancer patients and 139 non-cancer controls was conducted in li ... | 2005 | 15650879 |
| helicobacter infection, chronic inflammation, and the development of malignancy. | chronic infection with helicobacter pylori affects approximately half the world and results in malignancy in a small subset of this population. although the frequency of infection in developed nations is falling with a resultant decline in h. pylori-associated peptic ulcer disease, gastric cancer remains the second major cause of cancer death worldwide, with h. pylori infection being a major attributable factor in the development of gastric cancer. this review provides an update on the pathogene ... | 2005 | 15687882 |
| the sialic acid binding saba adhesin of helicobacter pylori is essential for nonopsonic activation of human neutrophils. | infiltration of neutrophils and monocytes into the gastric mucosa is a hallmark of chronic gastritis caused by helicobacter pylori. certain h. pylori strains nonopsonized stimulate neutrophils to production of reactive oxygen species causing oxidative damage of the gastric epithelium. here, the contribution of some h. pylori virulence factors, the blood group antigen-binding adhesin baba, the sialic acid-binding adhesin saba, the neutrophil-activating protein hp-nap, and the vacuolating cytotoxi ... | 2005 | 15689619 |
| the effect of ascorbic acid on helicobacter pylori induced cyclooxygenase 2 expression and prostaglandin e2 production by gastric epithelial cells in vitro. | cyclooxygenase 2 (cox-2) is induced by the presence of helicobacter pylori (h. pylori) on the gastric mucosa as part of the inflammatory response; this results in the synthesis of prostaglandins that amplify the local inflammatory response. the presence of h. pylori inhibits the secretion of ascorbate into the gastric lumen. interestingly, ascorbate inhibits the growth of h. pylori and low dietary levels are associated with an increased risk of gastric adenocarcinoma. we therefore investigated t ... | 2005 | 15691319 |
| concomitant dosing of famotidine with a triple therapy increases the cure rates of helicobacter pylori infections in patients with the homozygous extensive metabolizer genotype of cyp2c19. | proton-pump inhibitors, such as lansoprazole, are metabolized in the liver by cyp2c19 and cannot inhibit acid sufficiently in homozygous extensive metabolizers of cyp2c19. | 2005 | 15710002 |
| intracellular vaca is a valuable marker to predict whether helicobacter pylori induces progressive atrophic gastritis that is associated with the development of gastric cancer. | vaca was histochemically stained in biopsy specimen and was intracellularly and mainly located in fundic gland area. it is recognized gastric atrophy was observed in the h. pylori-positive patients with intracellular vaca compared with others. the aim of study is to understand the relationship between intracellular vaca and the progression of gastric atrophy that is associated with gastric cancer. biopsy specimens and sera were obtained from 364 people in their 50s and 60s without gastric cancer ... | 2005 | 15712638 |
| mutational analysis of metronidazole resistance in helicobacter pylori. | metronidazole is one of a few antibiotics effective in eliminating helicobacter pylori infection of the human stomach. several chromosomal loci have been implicated in resistance to this drug. saturation transposon mutagenesis of the h. pylori genome revealed inactivation of the rdxa gene as uniquely able to confer metronidazole resistance. | 2005 | 15728938 |
| helicobacter pylori induces interleukin-8 secretion by toll-like receptor 2- and toll-like receptor 5-dependent and -independent pathways. | helicobacter pylori is an important human pathogen that causes gastritis and is strongly associated with gastric ulcers, gastric adenocarcinomas, and mucosa-associated lymphoid tissue lymphomas. in response to h. pylori, interleukin-8 (il-8) is secreted from host cells to attract components of the innate and adaptive immune systems to the site of infection. toll-like receptor 2 (tlr2) and tlr5 have been shown to recognize h. pylori and to initiate signaling pathways that result in enhanced activ ... | 2005 | 15731050 |
| rapid loss of motility of helicobacter pylori in the gastric lumen in vivo. | the human pathogen helicobacter pylori has infected more than half of the world's population. nevertheless, the first step of infection, the acute colonization of the gastric mucus, is poorly understood. for successful colonization, h. pylori must retain active motility in the gastric lumen until it reaches the safety of the mucus layer. to identify the factors determining the acute colonization, we inserted bacteria into the stomach of anesthetized mongolian gerbils. we adjusted the gastric jui ... | 2005 | 15731057 |
| colonization and inflammation deficiencies in mongolian gerbils infected by helicobacter pylori chemotaxis mutants. | helicobacter pylori causes disease in the human stomach and in mouse and gerbil stomach models. previous results have shown that motility is critical for h. pylori to colonize mice, gerbils, and other animal models. the role of chemotaxis, however, in colonization and disease is less well understood. two genes in the h. pylori chemotaxis pathway, chey and tlpb, which encode the chemotaxis response regulator and a methyl-accepting chemoreceptor, respectively, were disrupted. the chey mutation was ... | 2005 | 15731083 |
| genetic polymorphisms of cyp2e1, gstt1, gstp1, gstm1, aldh2, and odc and the risk of advanced precancerous gastric lesions in a chinese population. | there have been few studies of the associations of genetic polymorphisms with precancerous gastric lesions. we conducted a cross-sectional study to compare the prevalences of several genetic polymorphisms in 302 subjects with mild chronic atrophic gastritis with prevalences in 606 subjects with deep intestinal metaplasia or dysplasia. this stratified random sample of 908 subjects was selected and analyzed for genetic polymorphisms from 2,628 individuals who had gastric biopsies with histopatholo ... | 2005 | 15734972 |
| morphological changes in human gastric tumours after eradication therapy of helicobacter pylori in a short-term follow-up. | it is controversial as to whether the development of gastric cancer is influenced by helicobacter pylori eradication. if eradication itself influences the tumour morphology, this may affect the tumour discovery rate. | 2005 | 15740539 |
| gene expression in gastric biopsies from patients infected with helicobacter pylori. | helicobacter pylori infection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. in this study the effects of h. pylori infection on host gene expression in the gastric antral mucosa in patients were examined. | 2004 | 15742995 |
| novel binding epitope for helicobacter pylori found in neolacto carbohydrate chains: structure and cross-binding properties. | helicobacter pylori is a bacterium that colonizes the stomach of a majority of the global human population causing common gastric diseases like ulcers and cancer. it has an unusually complex pattern of binding to various host glycoconjugates including interaction with sialylated, sulfated, and fucosylated sequences. the present study describes an additional binding epitope comprising the neolacto internal sequence of glcnacbeta3-galbeta4glcnacbeta. the binding was detected on tlc plates as an in ... | 2005 | 15743770 |
| detection of helicobacter pylori dna in human faeces and water with different levels of faecal pollution in the north-east of spain. | to assess the role of water in the faecal transmission of helicobacter pylori by detecting the dna of this pathogen in human faecal samples and environmental water samples with a range of faecal pollution from the north-east of spain. | 2005 | 15752335 |
| towards a new therapeutic target: helicobacter pylori flavodoxin. | helicobacter pylori flavodoxin is the electronic acceptor of the pyruvate-oxidoreductase complex (por) that catalyzes pyruvate oxidative decarboxilation. inactivation of this metabolic route precludes bacterial survival. because flavodoxin is not present in the human host, substances interfering electronic transport from por might be well suited for eradication therapies against the bacterium. h. pylori flavodoxin presents a peculiar cofactor (fmn) binding site, compared to other known flavodoxi ... | 2005 | 15752617 |
| interaction of amoxicillin with dna in human lymphocytes and h. pylori-infected and non-infected gastric mucosa cells. | amoxicillin is a penicillin derivative belonging to a group of beta-lactam antibiotics used in helicobacter pylori eradication. clinical application of amoxicillin is underlined by its antibacterial activity, but little is known about its interaction with dna of human cells. using the alkaline comet assay we investigated the genotoxicity of amoxicillin in human peripheral blood lymphocytes as well as in h. pylori-infected and non-infected human gastric mucosa cells. to assess the role of reactiv ... | 2005 | 15766919 |
| long-term strict monitoring of plasma ghrelin and other serological markers of gastric diseases after helicobacter pylori eradication. | we report on a case of chronic atrophic gastritis in which the serological markers of gastric diseases were strictly monitored for 2 years after successful helicobacter pylori (h. pylori) eradication. a 31-year-old man with upper abdominal pain was diagnosed as having h. pylori infection. laboratory examination revealed low serum levels of pepsinogen (pg) i, low pg i/ii ratio, and low plasma levels of ghrelin. upper gastrointestinal endoscopy revealed severe corpus-dominant atrophic gastritis. h ... | 2005 | 15782980 |
| helicobacter pylori in patients can be killed by visible light. | helicobacter pylori colonizes the mucus layer of the human stomach and may cause peptic ulcer and adenocarcinoma. novel antimicrobial approaches are sought due to the occurrence of antibiotic resistance and consequent treatment failure. we report here that h. pylori is susceptible to inactivation by blue light. | 2005 | 15791671 |
| immune subversion by helicobacter pylori. | to maintain prolonged colonization of the human gastric mucosa, helicobacter pylori must avoid both innate and adaptive immune responses. during its long coexistence with humans, it has evolved complex strategies to maintain a mild inflammation of the gastric epithelium while limiting the extent of immune effector activity. severe disease, associated with bacterial colonization, might reflect loss of this control. several mechanisms and the bacterial factors involved in immune subversion have, i ... | 2005 | 15797510 |
| evaluation of the stool antigen test for helicobacter pylori in children and adolescents. | the stool antigen test for helicobacter pylori is a noninvasive immunoassay to diagnose active infection with helicobacter pylori in human fecal samples. its performance in children and teenagers has been tested in some developed countries, showing a sensitivity and specificity above 90%, however, its accuracy in developing countries and in children under 6 years is not well established. to determine the accuracy of the test for diagnosing helicobacter pylori infection in children and teenagers, ... | 2005 | 15810625 |
| fibrin glue, healing of gastric mucosal injury, and expression of growth factors: results from a human in vivo study. | fibrin glue is used in the endoscopic therapy of bleeding ulcerations. accelerated closure of ulcers has been attributed to this treatment; the biologic reason, however, remains unclear. | 2005 | 15812409 |
| identification of two new helicobacter pylori surface proteins involved in attachment to epithelial cell lines. | helicobacter pylori causes the development of gastritis, gastric ulcers and adenocarcinomas in humans. the establishment of infection is influenced by adherence to the gastric epithelium, and several bacterial adhesins and host cell receptors have been identified. h. pylori recognize the lewis(b) receptor through the baba adhesin but also readily adhere to epithelia in the absence of the lewis(b) epitope, demonstrating the relevance of additional adhesive interactions. this study presents a nove ... | 2005 | 15824418 |
| difficulties in assessing cytomegalovirus-associated gastric perforation in an hiv-infected patient. | active cytomegalovirus (cmv) infection is a common complication in advanced symptomatic human immunodeficiency virus (hiv) infection. cmv-induced intestinal perforations are hard to diagnose and may be observed throughout the gastrointestinal tract. isolated stomach perforation is exceptional. | 2005 | 15829006 |
| helicobacter pylori and other helicobacter species in gallbladder and liver of patients with chronic cholecystitis detected by immunological and molecular methods. | despite several recent reports on the detection of helicobacter dna in human bile, there are still uncertainties concerning the correlation of these findings with biliary tract and liver diseases. | 2005 | 15841721 |
| primerselect: a transcriptome-wide oligonucleotide primer pair design program for kinetic rt-pcr-based transcript profiling. | we describe primerselect, a program capable of transcriptome-wide design of primer pairs for optimal performance in kinetic reverse-transcriptase polymerase chain reaction (rt-pcr). for the yeast saccharomyces cerevisiae, primerselect designs primer pairs for 86% of genomic open reading frames (orfs) using design criteria we previously established to be optimal for kinetic rt-pcr (krt-pcr)-based transcript quantitation. primer pairs designed by primerselect for 230 yeast orfs were evaluated for ... | 2005 | 15865983 |
| macrolide-affected toll-like receptor 4 expression from helicobacter pylori-infected monocytes does not modify interleukin-8 production. | macrolide antibiotics have an anti-inflammatory effect by suppressing lipopolysaccharide-induced il-8 production. il-8 secretion from monocytes is observed in helicobacter pylori infection. although cag gene products are known to induce il-8 secretion, whether other bacterial substances can initiate the reaction is not determined. in this study, we show that clarithromycin induced down-regulation of toll-like receptor 4 expression and did not lead to a decrease in il-8 production and h. pylori l ... | 2005 | 15866212 |
| restriction of dna encoding selectable markers decreases the transformation efficiency of helicobacter pylori. | helicobacter pylori populations recovered from the human stomach display extensive recombination and quasispecies development, and this suggests frequent exchange of dna between different strains in vivo. in vitro, however, most h. pylori strains display restriction to the uptake of non-self dna, as measured using selectable markers, regardless of their competency for transformation with self dna. we have examined the effect of different selectable markers on double-crossover recombination effic ... | 2005 | 15866218 |
| involvement of proinflammatory cytokines il-1beta and il-6 in progression of human gastric carcinoma. | gastric carcinoma occurs in response to chronic inflammation of gastric mucosa infected with helicobacter pylori. it is not known how cytokines affect the growth and progression of gastric carcinoma. | 2005 | 15868900 |
| helicobacter pylori eradication induces marked increase in h+/k+-adenosine triphosphatase expression without altering parietal cell number in human gastric mucosa. | gastric acid secretion is downregulated by helicobacter pylori infection and upregulated after its eradication, but the mechanisms are still unclear. we examined the effects of h pylori eradication on the number of parietal cells and on expression of molecules functioning in acid secretion in the human gastric mucosa. | 2006 | 15872000 |
| identification of non-helicobacter pylori spiral organisms in gastric samples from humans, dogs, and cats. | tightly coiled bacteria are a rare cause of gastric pathology in humans and represent a mixture of species for which a zoonotic origin is suspected. similar organisms are common inhabitants of the gastric mucosae of carnivores and pigs. it was the goal of the present study to determine the actual occurrence of each individual helicobacter species in human, canine, and feline stomachs in order to better understand the possible zoonotic significance. gastric biopsy samples from humans with histolo ... | 2005 | 15872252 |
| synergism of helicobacter pylori infection and stress on the augmentation of gastric mucosal damage and its prevention with alpha-tocopherol. | despite evidence that helicobacter pylori (h. pylori) infection is closely associated with stress in gastric ulcer patients, the underlying mechanism why ulcer recurrence after stress is augmented especially in patients with h. pylori remains unknown. in this study, we found that oxidative stress played a critical role in the augmented mucosal damage provoked by water immersion restraint stress (wirs) in h. pylori infection and that an antioxidant, alpha-tocopherol, could ameliorate the aggravat ... | 2005 | 15890619 |
| iron-responsive regulation of the helicobacter pylori iron-cofactored superoxide dismutase sodb is mediated by fur. | maintaining iron homeostasis is a necessity for all living organisms, as free iron augments the generation of reactive oxygen species like superoxide anions, at the risk of subsequent lethal cellular damage. the iron-responsive regulator fur controls iron metabolism in many bacteria, including the important human pathogen helicobacter pylori, and thus is directly or indirectly involved in regulation of oxidative stress defense. here we demonstrate that fur is a direct regulator of the h. pylori ... | 2005 | 15901691 |
| upregulated akt signaling adjacent to gastric cancers: implications for screening and chemoprevention. | most gastric adenocarcinomas arise as a longterm complication of helicobacter pylori infection of the stomach, but the high prevalence of this infection limits the cost-effectiveness of antibiotic eradication as a cancer prevention strategy. here we have used phosphorylation-specific antibodies against the akt kinase consensus sequence to detect downstream substrates of this oncogenic signaling pathway in normal and malignant gastric tissues. in vitro studies confirm that phosphorylation of akt ... | 2004 | 15922857 |
| polarized expression of cd74 by gastric epithelial cells. | cd74 is known as the major histocompatibility complex (mhc) class ii-associated invariant chain (ii) that regulates the cell biology and functions of mhc class ii molecules. class ii mhc and ii expression was believed to be restricted to classical antigen-presenting cells (apc); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class ii mhc molecules. given the importance of ii in the biology of class ii mhc, we sought to exami ... | 2005 | 15923369 |
| enhancing health benefits of berries through phenolic antioxidant enrichment: focus on cranberry. | emerging epidemiological evidence is increasingly pointing to the beneficial effects of fruits and vegetables in managing chronic and infectious diseases. these beneficial effects are now suggested to be due to the constituent phenolic phytochemicals having antioxidant activity. cranberry like other fruits is also rich in phenolic phytochemicals such as phenolic acids, flavonoids and ellagic acid. consumption of cranberry has been historically been linked to lower incidences of urinary tract inf ... | 2005 | 15927928 |
| helicobacter pylori promote gastric cancer cells invasion through a nf-kappab and cox-2-mediated pathway. | to examine the effects of helicobacter pylori (h pylori) infection on the invasiveness of gastric cancer cells, and to elucidate its mechanism. | 2005 | 15929167 |
| interleukin 12 gene polymorphisms enhance gastric cancer risk in h pylori infected individuals. | | 2005 | 15937086 |
| long-term persistence of molecular disease after histological remission in low-grade gastric malt lymphoma treated with h. pylori eradication. lack of association with translocation t(11;18): a 10-year updated follow-up of a prospective study. | localized low-grade gastric mucosa-associated lymphoid tissue (malt) lymphoma can regress after helicobacter pylori eradication, but igv(h) gene monoclonality may persist. we studied the long-term histological and molecular follow-up of 24 patients and the possible association of t(11;18) with the persistent monoclonality. | 2005 | 15946976 |
| alpha-catenin expression is decreased in patients with gastric carcinoma. | to assess the expression of alpha-catenin in gastric carcinoma and to determine the role of alpha-catenin expression in gastric carcinogenesis. | 2005 | 15948257 |
| comparative proteome analysis of untreated and helicobacter pylori-treated hepg2. | to investigate the pathological effect of helicobacter pylori (h pylori) on human hepatic cells, proteomic methods were used to find and to identify proteins that were overexpressed in hepg2 cells treated by h pylori. | 2005 | 15948260 |
| antimicrobial potential of probiotic lactic acid bacteria. | antibacterial activity is an important characteristic of probiotics. a possible way to achieve this antibacterial activity is through the production of bacteriocins. this study shows that the commercial probiotic strains lactobacillus johnsonii la1 and lactobacillus casei yit 9029 produce bacteriocins. the nature of the antimicrobial compound could be derived from its behaviour: a narrow inhibitory spectrum (only active against closely related strains), loss of activity when treated with protein ... | 2001 | 15954651 |
| evasion of toll-like receptor 5 by flagellated bacteria. | toll-like receptor 5 (tlr5) recognizes an evolutionarily conserved site on bacterial flagellin that is required for flagellar filament assembly and motility. the alpha and epsilon proteobacteria, including the important human pathogens campylobacter jejuni, helicobacter pylori, and bartonella bacilliformis, require flagellar motility to efficiently infect mammalian hosts. in this study, we demonstrate that these bacteria make flagellin molecules that are not recognized by tlr5. we map the site r ... | 2005 | 15956202 |
| effect of cyp2c19 and mdr1 polymorphisms on cure rate in patients with acid-related disorders with helicobacter pylori infection. | a proton pump inhibitor (ppi) plus two antibiotics (amoxicillin and either clarithromycin or metronidazole) are recommended for treatment of acid-related disorders with helicobacter pylori (h. pylori) infection. the aim of this pharmacogenetic study was to evaluate the efficacy of triple therapy with ppis on eradication of h. pylori infection in relation to cytochrome p450 2c19 (cyp2c19) and p-glycoprotein (mdr1) gene polymorphisms. the retrospective study involved 70 polish caucasian patients w ... | 2005 | 15976989 |
| infection with helicobacter pylori affects all major secretory cell populations in the human antrum. | we have investigated how gastric h. pylori infection affects antrum secretory cell types by studying the expression of secretory proteins in antrum epithelium. antrum biopsy specimens were prospectively collected from 102 individuals (49 h. pylori-infected). immunohistochemistry was performed for secretory mucins (muc5ac, muc5b, muc6), trefoil factor family (tff)-peptides (tff1, tff2), endocrine peptides (gastrin, chromogranin a), and proliferating cells (ki-67). protein expression was quantifie ... | 2005 | 15986858 |
| [epidemiologic, pathologic, diagnostic and clinical features and therapy of helicobacter pylori infection based on latest data]. | helicobacter pylori is one of the most common human microorganisms. the outcome of its acquisition is not predictable, nevertheless a great amount of knowledge has accumulated concerning the virulence factors of the bacteria and the diseases associated with the infection. in most cases, this indigenous bacterium of the human stomach does not cause serious illnesses and the consequences of the infection depend on host and environmental factors. a more comprehensive insight into bacterial pathogen ... | 2005 | 15997663 |
| helicobacter pylori induce neutrophil transendothelial migration: role of the bacterial hp-nap. | continuous recruitment of neutrophils into the inflamed gastric mucosal tissue is a hallmark of helicobacter pylori infection in humans. in this study, we examined the ability of h. pylori to induce transendothelial migration of neutrophils using a transwell system consisting of a cultured monolayer of human endothelial cells as barrier between two chambers. we showed for the first time that live h. pylori, but not formalin-killed bacteria, induced a significantly increased transendothelial migr ... | 2005 | 16000239 |
| molecular cloning and functional expression of a novel helicobacter pylori alpha-1,4 fucosyltransferase. | helicobacter pylori is an important human pathogen which causes both gastric and duodenal ulcers and is associated with gastric cancer and lymphoma. this microorganism synthesizes fucosylated oligosaccharides, predominantly the galb-1,4glcnac (type ii) blood group antigens lewis x and y, whereas a small population also expresses the galb-1,3glcnac (type i) blood group antigens lewis a and b. these carbohydrate structures are known to mimic host cell antigens and permit the bacteria to escape fro ... | 2005 | 16000696 |
| hyperemesis gravidarum, a literature review. | hyperemesis gravidarum (hg) is a condition causing severe nausea and vomiting in early pregnancy often resulting in hospital admission. the incidence of hg is approximately 0.5% of live births, said to be higher in multiple pregnancies, hydatidiform mole and other conditions associated with increased pregnancy hormone levels. both the aetiology and pathogenesis of hg remain unknown. we conducted a literature review (1966-now) to summarize the current evidence on the aetiology and pathogenesis of ... | 2005 | 16006438 |
| human t-lymphotropic virus type i infection and idiopathic thrombocytopnic purpura. | human t-lymphotropic virus type i (htlv-i) is the causative agent in adult t-cell leukemia and htlv-i associated myelopathy. some other diseases such as uveitis, chronic thyroiditis, sjögren syndrome, arthritis, acute myeloid leukemia and myelodysplastic syndrome may be also associated with htlv-i. several case reports have suggested the possible combination of idiopathic thrombocytopenic purpura (itp) and htlv-i infection. in these studies and from our current report, we found 17 patients (22.1 ... | 2005 | 16019454 |
| synergistic enhancement of toll-like receptor responses by nod1 activation. | nod1 is an intracellular pattern-recognition receptor specific for gram-negative peptidoglycan that is important in host response to infections (e.g. helicobacter pylori and shigella flexneri). genetic variation in nod1 predisposes to asthma and inflammatory bowel disease. functional responses have not previously been studied in primary human cells. nod1 activation by low nanomolar concentrations of the specific muropeptide ligand m-tridap induced minimal human peripheral blood mononuclear cell ... | 2005 | 16021603 |
| up-regulation of cathepsin x in helicobacter pylori gastritis and gastric cancer. | recently, we identified increased cathepsin x expression in h. pylori-infected gastric mucosa. here, we describe further up-regulation in gastric cancer and report on the role of inflammatory cytokines required for cathepsin x up-regulation in h. pylori-infected gastric mucosa, as well as on consequences for cellular invasion. biopsy specimens were taken from the antrum, corpus and cardia of h. pylori-infected and non-infected patients. gastric cancer samples were obtained from patients undergoi ... | 2005 | 16025436 |
| association between serum pepsinogens and polymorphismof ptpn11 encoding shp-2 among helicobacter pylori seropositive japanese. | helicobacter pylori (h. pylori) plays a crucial role in the development of gastric atrophy and cancer, and caga-positive strains, which are universal in japan, increase the risk of these outcomes substantially. the caga protein is injected from attached h. pylori into gastric epithelial cells and undergoes src-dependent tyrosine phosphorylation and activation of the eukaryotic phosphatase shp-2. the caga/shp-2 interactions elicit cellular changes that increase the risk of carcinogenesis. we inve ... | 2006 | 16032704 |
| isoprenoid biosynthetic pathways as anti-infective drug targets. | ipp (isopentenyl diphosphate) and dmapp (dimethylallyl diphosphate) serve as the universal precursors for the biosynthesis of isoprenoids. besides the well-known mevalonate pathway, the existence of a second biosynthetic pathway conducive to ipp and dmapp formation through 1-deoxy-d-xylulose 5-phosphate and 2c-methyl-d-erythritol 4-phosphate was discovered approx. 10 years ago in plants and certain eubacteria. it is now known that this pathway is widely distributed in the bacterial kingdom inclu ... | 2005 | 16042599 |
| microsatellite instability and gastric non-invasive neoplasia in a high risk population in cesena, italy. | in the natural history of gastric cancer, non-invasive neoplasia (nin) precedes invasive carcinoma. a histological classification of gastric nin has recently been proposed by a world health organisation international panel of experts. genetic instability is known to be among the molecular pathways involved in gastric oncogenesis. in this retrospective cross sectional study, microsatellite instability (msi) was analysed in a consecutive series of nin and nin related histological alterations from ... | 2005 | 16049280 |
| an overview of acetic acid ulcer models--the history and state of the art of peptic ulcer research. | four types of experimental chronic ulcer models, named acetic acid ulcer models, have been developed to examine the healing process of peptic ulcers, screen anti-ulcer drugs, and better evaluate the adverse effects of various anti-inflammatory drugs on the gastrointestinal mucosa. the model easily and reliably produces round, deep ulcers in the stomach and duodenum, allowing acetic acid ulcer production in mice, rats, mongolian gerbils, guinea pigs, cats, dogs, miniature pigs, and monkeys. these ... | 2005 | 16079471 |
| cytokeratin 7 and 20 expression in intestinal metaplasia of the distal oesophagus: relationship to gastro-oesophageal reflux disease. | intestinal metaplasia and gastro-oesophageal reflux disease typify classical barrett's oesophagus. cytokeratin (ck) 7 and 20 phenotypes differentiate intestinal metaplasia in long segment barrett's oesophagus from gastric intestinal metaplasia. this study examines the relationship between ck7/20 phenotypes and reflux disease in intestinal metaplasia of the distal oesophagus. | 2005 | 16115227 |
| the usefulness of stable isotopes in nutrition and human health: the application of mass spectrometry and 13c-breath tests to detect helicobacter pylori infection. | the interest in helicobacter pylori has escalated in recent years. h. pylori may produce a chronic gastric infection which is usually life-long and many epidemiological studies have shown that this is the most common bacterial infection throughout the world involving 50% of the world population. thus, it is clear that the diagnosis of h. pylori infection represents at least a key step in the management of many of the patients referred to the gastroenterologist. additionally, due to the wide rang ... | 2004 | 16119720 |
| cloning of helicobacter pylori urease subunit b gene and its expression in tobacco (nicotiana tabacum l.). | vaccines produced by transgenic plants would have the potential to change the traditional means of production and inoculation of vaccines, and to reduce the cost of vaccine production. in the present study, an ureb antigen gene from a new helicobacter pylori strain zjc02 was cloned into the binary vector pbi121 which contains a camv35s promoter and a kanamycin resistance gene, and then transformed ureb into tobacco leaf-disc by agrobacterium-mediated method. a total of 50 regenerated plants with ... | 2005 | 16133345 |
| host epithelial interactions with helicobacter pylori: a role for disrupted gastric barrier function in the clinical outcome of infection? | infection of the human stomach with helicobacter pylori may develop into gastritis, ulceration, adenocarcinoma and mucosal lymphomas. the pathogenic mechanisms that determine the clinical outcome from this microbial-epithelial interaction remain poorly understood. an increasing number of reports suggests that disruptions of epithelial barrier function may contribute to pathology and postinfectious complications in a variety of gastrointestinal infections. the aim of this review is to critically ... | 2005 | 16151546 |
| molecular pathogenesis of mucosa-associated lymphoid tissue lymphoma. | extranodal marginal zone b-cell lymphomas of mucosa-associated lymphoid tissue (malt) type occur in a number of anatomic sites, but share overlapping morphologic and immunophenotypic features. helicobacter pylori infection has been identified as an etiologic factor in gastric malt lymphoma, and a growing list of other infectious organisms have recently been shown to be associated with malt lymphomas at other anatomic sites. although cause and effect has not been established for most of these inf ... | 2005 | 16155022 |
| deciphering host migrations and origins by means of their microbes. | mitochondrial dna and microsatellite sequences are powerful genetic markers for inferring the genealogy and the population genetic structure of animals but they have only limited resolution for organisms that display low genetic variability due to recent strong bottlenecks. an alternative source of data for deciphering migrations and origins in genetically uniform hosts can be provided by some of their microbes, if their evolutionary history correlates closely with that of the host. in this revi ... | 2005 | 16156803 |
| bioactivities of anastasia black (russian sweet pepper). | anastasia black (russian sweet pepper) of capsicum annuum l. var. angulosum mill. (solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of twenty-three fractions by silica gel or octadecylsilane (ods; c18) column chromatography. these extracts and fractions were investigated for their cytotoxicity, anti-human immunodeficiency virus (hiv), anti-helicobacter pylori (h. pylori), urease inhibition and multidrug r ... | 2005 | 16158935 |
| the -251t allele of the interleukin-8 promoter is associated with increased risk of gastric carcinoma featuring diffuse-type histopathology in chinese population. | persistent interleukin-8 (il-8) production contributes to chronic inflammation of the stomach. the proinflammatory il-1beta polymorphisms, which enhance the cytokine production, are associated with increased risk of gastric cancer. the -251a/t polymorphism of the il-8 promoter is involved in several human diseases. particularly, the -251a is associated with decreased risk of colorectal cancer. we aimed to determine whether the -251 allele resulting in high il-8 expression was associated with inc ... | 2005 | 16166417 |
| mutation and recombination in helicobacter pylori: mechanisms and role in generating strain diversity. | the pathogenic bacterium helicobacter pylori infects half of the human population and it is one of the most genetically diverse bacterial species known. the unusual combination of high frequencies of mutation and recombination events together with a very small size of dna fragments imported into the genome after recombination, contribute to its genetic variability. in this review article, we discuss the genetic variability of h. pylori, the mechanisms that generate diversity, and the potential r ... | 2005 | 16173496 |
| helicobacter pylori and the innate immune system. | since its discovery, helicobacter pylori surprises us by its ability for life-long chronic persistence, proliferation, and probably active adaptation in the unfavourable niche of the human stomach, without being eliminated by the defence systems of the human body. this minireview highlights recent developments about the interaction of h. pylori with the innate immune system, and makes a case that evasion and possibly suppression of innate immune responses play an important role for the active su ... | 2005 | 16173499 |
| the oxysterol-binding protein homologue orp1l interacts with rab7 and alters functional properties of late endocytic compartments. | orp1l is a member of the human oxysterol-binding protein (osbp) family. orp1l localizes to late endosomes (les)/lysosomes, colocalizing with the gtpases rab7 and rab9 and lysosome-associated membrane protein-1. we demonstrate that orp1l interacts physically with rab7, preferentially with its gtp-bound form, and provide evidence that orp1l stabilizes gtp-bound rab7 on les/lysosomes. the rab7-binding determinant is mapped to the ankyrin repeat (ank) region of orp1l. the pleckstrin homology domain ... | 2005 | 16176980 |
| acid-induced activation of the urease promoters is mediated directly by the arsrs two-component system of helicobacter pylori. | the nickel-containing enzyme urease is an essential colonization factor of the human gastric pathogen helicobacter pylori which enables the bacteria to survive the low-ph conditions of the stomach. transcription of the urease genes is positively controlled in response to increasing concentrations of nickel ions and acidic ph. here we demonstrate that acid-induced transcription of the urease genes is mediated directly by the arsrs two-component system. footprint analyses identify binding sites of ... | 2005 | 16177315 |
| overexpression of caspase recruitment domain (card) membrane-associated guanylate kinase 1 (carma1) and card9 in primary gastric b-cell lymphoma. | although caspase recruitment domain (card) membrane-associated guanylate kinase (maguk) protein 1 (carma1) and card9 play important roles in lymphocyte activation, the significance of carma1 and card9 in the pathogenesis of gastric mucosa-associated lymphoid tissue (malt) lymphoma remains to be elucidated. | 2005 | 16177990 |
| helicobacter pylori and extragastric diseases--other helicobacters. | the involvement of helicobacter pylori in the pathogenesis of extragastric diseases continues to be an interesting topic in the field of helicobacter-related pathology. although conflicting findings have been reported for most of the disorders, a role of h. pylori seems to be important especially for the development of cardiovascular and hematologic disorders. previously isolated human and animal helicobacter sp. flexispira and "helicobacter heilmannii" strains have been validated using polyphas ... | 2005 | 16178972 |
| [pathogenesis of helicobacter pylori infection]. | helicobacter pylori (h. pylori), a long term colonizer of human stomach is known to infect a half of mankind. gastric and duodenal ulcer, gastric adenocarcinoma and malt lymphoma develop in a subset of infected individuals. pathogenesis of h. pylori infection is based on the long-term host to bacterial interaction and affected by the virulence factors of the bacterium, environmental and host factors (age, sex, blood type). mucosal inflammation is the basic principle mechanism underlying the dise ... | 2005 | 16179834 |
| h. pylori infection increases levels of exhaled nitrate. | helicobacter pylori infection is one of the most common chronic bacterial infections worldwide. despite the existence of a breath test for the diagnosis of h. pylori infection, no study has described the composition of volatile compounds, especially the levels of nitrate, in the exhaled air of patients with h. pylori infection. | 2005 | 16181348 |
| il1rn polymorphism and caga-positive helicobacter pylori strains increase the risk of duodenal ulcer in children. | duodenal ulcers in children are associated with helicobacter pylori gastric infection with caga-positive strains, but factors linked to the host are poorly known. the authors evaluated the role of proinflammatory interleukin-1 gene cluster polymorphisms in the pathogenesis of duodenal ulcer. they studied prospectively 437 children 1 to years old, 209 of whom were h. pylori positive and 228 of whom were h. pylori negative. il1b-511-c/t, -31t/c, and il1rn variable number of tandem repeats were gen ... | 2005 | 16183821 |
| helicobacter pylori-specific immune response in maternal serum, cord blood, and human milk among mothers with and without current helicobacter pylori infection. | we assessed the patterns of helicobacter pylori (h. pylori) specific maternal antibodies in maternal serum, cord blood, and milk, which might play a role in prevention of h. pylori infection because transferred to the infant. between november 2000 and november 2001, mothers were recruited after delivery of their offspring. h. pylori infection status was determined by 13c-urea breath test (ubt). specific h. pylori antibody profiles were analysed using commercial h. pylori-specific enzyme-linked i ... | 2005 | 16183830 |
| alteration of cytochrome p-450 and glutathione s-transferase activity in normal and malignant human stomach. | cytochrome p-450 and glutathione s-transferase (gst) activities were investigated in stomach tumor and tumor-adjacent tissues of patients (n = 211) with gastric adenocarcinoma, and in the stomach tissues of unaffected individuals (normal tissues, n = 113). a significant reduction in total cytochrome p-450 activity was observed in tumor and tumor-adjacent tissues versus normal stomach. in all cases, cytochrome p-450 activity was significantly higher in males than in females. in the case of smoker ... | 2005 | 16195217 |
| influence of antigen on the development of malt lymphoma. | mucosa-associated lymphoid tissue (malt) b-cell lymphomas develop in the context of autoimmune or chronic inflammations like helicobacter pylori-induced gastritis. remission of most gastric malt lymphomas after eradication of h pylori links tumor cell proliferation to antigen-induced inflammation and the need for antigenic contact. furthermore, the tumor cells correspond to antigen-activated memory b cells. to investigate the reactivity of the tumor immunoglobulins we employed in vitro-generated ... | 2006 | 16204314 |
| hla-dqa1*0103-dqb1*0601 haplotype and helicobacter pylori-positive gastric mucosa-associated lymphoid tissue lymphoma. | immune responses to helicobacter pylori are important in the pathogenesis of gastric mucosa-associated lymphoid tissue (malt) lymphoma. in this retrospective case study, we investigated whether certain alleles and haplotypes of major histocompatibility complex genes are associated with gastric malt lymphoma and the efficacy of h pylori eradication therapy on the lymphoma. | 2005 | 16234023 |
| suppressive effects of fruit-juice concentrate of prunus mume sieb. et zucc. (japanese apricot, ume) on helicobacter pylori-induced glandular stomach lesions in mongolian gerbils. | helicobacter pylori (hp) infection is an important factor in human gastric disorders, including chronic active gastritis, peptic ulcers, intestinal metaplasia and cancer. since epidemiologic studies overwhelmingly agree on a protective influence of fruits and vegetables in reducing the risk of gastric neoplasia and processed foods made from prunus mume sieb. et zucc. (japanese apricot or "ume" in japanese) are traditionally known for their miscellaneous medical effects, in the present study we i ... | 2005 | 16235996 |
| helicobacter pylori-stimulated interleukin-8 (il-8) promotes cell proliferation through transactivation of epidermal growth factor receptor (egfr) by disintegrin and metalloproteinase (adam) activation. | helicobacter pylori infection increases the risk of hyperplastic polyps and gastric cancer, but the mechanisms remain to be elucidated. h. pylori was recently shown to transactivate epidermal growth factor receptor (egfr) through metalloprotease stimulation. the present study was designed to investigate the effect of interleukin-8 (il-8) induced by h. pylori infection on egfr transactivation and epithelial cell growth. h. pylori sydney strain 1 (ss1) having wild-type cag(+)a was used. phospho-eg ... | 2005 | 16240219 |
| multiplex-touchdown pcr assay for the detection and genotyping of helicobacter pylori from artificially contaminated sheep milk. | helicobacter pylori (hp) is an organism commonly present worldwide in the human population, sometimes causing serious illnesses such as duodenal and gastric ulcers, adenocarcinoma of the stomach, and low-grade b-cell mucosa-associated lymphoid tissue lymphoma of the stomach. this article describes a multiplex-touchdown pcr method for the identification and genotyping (vaca-s1/m1, sl/m2, and s2/m2-and caga genes) of hp directly from sheep milk artificially contaminated with hp strains from human ... | 2005 | 16245719 |
| anti-inflammatory effect of activated protein c in gastric epithelial cells. | it has been previously demonstrated that activated protein c (apc) plays an important role in the inhibition of inflammation in the gastric mucosa from patients with helicobacter pylori infection. however, the role of gastric epithelial cells in the anti-inflammatory activity of apc remains unknown. in the present study, we evaluated the anti-inflammatory activity of apc and the expression of thrombomodulin (tm) and endothelial protein c receptor (epcr) in gastric epithelial cells. the gastric e ... | 2005 | 16246255 |
| hedgehog signaling pathway and gastric cancer. | hedgehog, wnt, fgf and bmp signaling pathways network together during embryogenesis, tissue regeneration, and carcinogenesis. aberrant activation of hedgehog signaling pathway leads to pathological consequences in a variety of human tumors, such as gastric cancer and pancreatic cancer. endoscopic mucosal resection (emr), endoscopic submucosal dissection (esd), surgical gastrectomy and chemotherapy are therapeutic options for gastric cancer; however, prognosis of advanced gastric cancer patient i ... | 2005 | 16258256 |
| the plasmodium protein network diverges from those of other eukaryotes. | plasmodium falciparum is the pathogen responsible for over 90% of human deaths from malaria. therefore, it has been the focus of a considerable research initiative, involving the complete dna sequencing of the genome, large-scale expression analyses, and protein characterization of its life-cycle stages. the plasmodium genome sequence is relatively distant from those of most other eukaryotes, with more than 60% of the 5,334 encoded proteins lacking any notable sequence similarity to other organi ... | 2005 | 16267557 |
| helicobacter pylori and gastric epithelial cells: from gastritis to cancer. | helicobacter pylori is a spiral, gram-negative rod-shaped pathogen that attaches to gastric epithelial cells in the human stomach and is a causative agent of chronic active gastritis, peptic ulcer and neoplasia. h. pylori is one of the most common pathogens afflicting humans and is the major environmental factor in the development of gastric cancer increasing from 4 to 6 folds the risk of its development. several specific virulence factors are implicated in the mechanism of h. pylori infection l ... | 2005 | 16270519 |
| upregulation of cathepsin w-expressing t cells is specific for autoimmune atrophic gastritis compared to other types of chronic gastritis. | to investigate a pathophysiological role of cathepsin w (catw), a putative thiol-dependent cysteine protease, which is specifically expressed in cytotoxic lymphocytes, in different types of chronic inflammation of the gastric mucosa. | 2005 | 16273605 |
| is the hypoxia-inducible factor pathway important in gastric cancer? | tumour hypoxia is well recognised in oncology to be a key factor resulting in treatment resistance and poor prognosis. hypoxia leads to the expression of a number of gene products that are involved in tumour progression, invasion and metastasis formation. the most important of these proteins is thought to be hypoxia-inducible factor-1alpha (hif-1alpha), which appears to be a master regulator of the cellular response to hypoxia. hif-1alpha expression is associated with a poor prognosis and treatm ... | 2005 | 16290133 |
| [helicobacter pylori caga and shp-2 tyrosine phosphatase]. | | 2005 | 16296318 |