| effects of lead contamination on soil microbial activity and rice physiological indices in soil-pb-rice (oryza sativa l.) system. | the effect of lead (pb) treatment on the soil microbial activities (soil microbial biomass and soil basal respiration) and rice physiological indices were studied by greenhouse pot experiment. pb was applied as lead acetate at six different levels in two different paddy soils, namely 0 (control), 100, 300, 500, 700, 900 mg kg-1 soil. the results showed that the application of pb at lower level (<300 mg kg-1) as lead acetate resulted in a slight increase in soil microbial activities compared with ... | 2006 | 16581104 |
| remediation of cadmium contamination in paddy soils by washing with chemicals: selection of washing chemicals. | the efficiencies of neutral salts, strong acids, and chelates were tested for extracting cadmium (cd) from three paddy soils. the higher the selectivity of the cations of the added neutral salts toward soil adsorption sites, the lower the ph in the extracts and the more soil cd could be extracted. in addition, soil carbon and nitrogen contents and mineral composition were closely associated with the amount of cd extracted. calcium chloride and iron(iii) chloride were selected as wash chemicals t ... | 2006 | 16580105 |
| cyanidin 3-glucoside and peonidin 3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo. | dietary polyphenols, including anthocyanins, are suggested to be involved in the protective effects of fruits and vegetables against cancer. however, anticancer effects of peonidin 3-glucoside have not been clearly demonstrated, with only limited studies being available concerning the inhibitory effect of cyanidin 3-glucoside for tumor cell growth. therefore, in this study, we have isolated and identified the two bioactive compounds, peonidin 3-glucoside and cyanidin 3-glucoside, from oryza sati ... | 2005 | 16573384 |
| ssr mapping of brown planthopper resistance gene bph9 in kaharamana, an indica rice (oryza sativa l.). | the brown planthopper (bph) is one of the most serious insects pests of rice, and the host resistance has been recognized as one of the most economic and effective measures for bph management. in this study, we conducted a molecular-based genetic analysis of bph9 in kaharamana, a sri lanka rice variety resistant to bph insects of east and southeast asia. an f2 segregating population composed of 180 plants was constructed from the cross between kaharamana and 02428, and each f2 plant was self-cro ... | 2006 | 16553215 |
| drtf: a database of rice transcription factors. | drtf contains 2025 putative transcription factors (tfs) in oryza sativa l. ssp. indica and 2384 in ssp. japonica, distributed in 63 families, identified by computational prediction and manual curation. it includes detailed annotations of each tf including sequence features, functional domains, gene ontology assignment, chromosomal localization, est and microarray expression information, as well as multiple sequence alignment of the dna-binding domains for each tf family. the database can be brow ... | 2006 | 16551659 |
| [a strategy based on comparative genomics to align ests of maize]. | in this study, a new strategy to locate ests on maize linkage groups was described. in the strategy, the rice (oryza sativa l.) genomic sequence database of was employed to locate maize est on rice linkage groups, and then to locate on maize linkage group by comparative genetics mapping between rice and maize genome. the aligned ests information should available for further study on genomics and gene cloning. as an example, 139 ests of maize were assayed, and 96 maize ests (69%) were homologous ... | 2006 | 16551603 |
| proteomic analyses of oryza sativa mature pollen reveal novel proteins associated with pollen germination and tube growth. | as a highly reduced organism, pollen performs specialized functions to generate and carry sperm into the ovule by its polarily growing pollen tube. yet the molecular genetic basis of these functions is poorly understood. here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of oryza sativa l. ssp japonica using a proteomic approach, 23% of them having more than one isoform. functional classification reveals that an overrepresentat ... | 2006 | 16548068 |
| accumulation, distribution and transformation of ddt and pcbs by phragmites australis and oryza sativa l.: i. whole plant study. | glasshouse experiments were conducted to determine the accumulation, distribution and transformation of o,p'-ddt, p,p'-ddt and pcbs by common reed (phragmites australis) and rice (oryza sativa l.) under hydroponic conditions. the culture solution was spiked with the organic pollutants and samples were collected daily. analysis of the plants at harvest showed that both species had removed ddt and pcbs from the solution. ddt appeared to have accumulated within p. australis by both passive adsorpti ... | 2017 | 16547765 |
| accumulation, distribution and transformation of ddt and pcbs by phragmites australis and oryza sativa l.: ii. enzyme study. | two wetland plant species, phragmites australis and oryza sativa, were grown in a glasshouse under hydroponics conditions. enzyme extracts from different parts of the plants were used to determine the transformation rate of o,p'-ddt, p,p'-ddt and pcbs. the organic pollutants were directly spiked into the enzyme extracts, and samples were collected every 30 min and analyzed with a gc-ecd. root extracts of p. australis readily degraded and transformed ddt and some pcb congeners with a low degree o ... | 2017 | 16547764 |
| selection under domestication: evidence for a sweep in the rice waxy genomic region. | rice (oryza sativa) was cultivated by asian neolithic farmers >11,000 years ago, and different cultures have selected for divergent starch qualities in the rice grain during and after the domestication process. an intron 1 splice donor site mutation of the waxy gene is responsible for the absence of amylose in glutinous rice varieties. this mutation appears to have also played an important role in the origin of low amylose, nonglutinous temperate japonica rice varieties, which form a primary com ... | 2006 | 16547098 |
| cytogenetic comparisons between a and g genomes in oryza using genomic in situ hybridization. | the genomic structures of oryza sativa (a genome) and o. meyeriana (g genome) were comparatively studied using bicolor genomic in situ hybridization (gish). gish was clearly able to discriminate between the chromosomes of o. sativa and o. meyeriana in the interspecific f1 hybrids without blocking dna, and co-hybridization was hardly detected. the average mitotic chromosome length of o. meyeriana was found to be 1.69 times that of o. sativa. a comparison of 4,6-diamidino-2-phenylindole staining s ... | 2006 | 16541124 |
| evidence for psii donor-side damage and photoinhibition induced by cadmium treatment on rice (oryza sativa l.). | the effects of cadmium (from 7.5 to 75 microm) on chloroplasts of rice were studied at the structural and biochemical level. loss of pigments, reduction of thylakoids and decrease in oxygen evolution and fv/fm ratio occur in leaves following cadmium treatment. however, the amount of photosystem ii reaction center proteins and that of its light harvesting complex is not affected, indicating that cadmium does not adversely influence the structural organization of this photosystem. in thylakoids is ... | 2006 | 16540337 |
| vernalization response in perennial ryegrass (lolium perenne l.) involves orthologues of diploid wheat (triticum monococcum) vrn1 and rice (oryza sativa) hd1. | flowering time is important when adapting crop plants to different environments. while high feeding quality of forage grasses is facilitated by repression of flowering, flowering should also be inducible to facilitate grass seed production. consequently, the identification and characterization of the genes controlling flowering time in forage grasses, including perennial ryegrass (lolium perenne l.), is of great interest. in this study, three candidate genes for vernalization response genes in p ... | 2006 | 16525886 |
| sensitivity of rice to ultraviolet-b radiation. | depletion of the stratospheric ozone layer leads to an increase in ultraviolet-b (uvb: 280-320 nm) radiation reaching the earth's surface, and the enhanced solar uvb radiation predicted by atmospheric models will result in reduction of growth and yield of crops in the future. over the last two decades, extensive studies of the physiological, biochemical and morphological effects of uvb in plants, as well as the mechanisms of uvb resistance, have been carried out. | 2006 | 16520342 |
| an accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina. | proteomic analysis of any biological system by two-dimensional gel electrophoresis (2-de) requires high resolution and high reproducibility. the results presented here demonstrate the reproducible and accurate separation of rice (oryza sativa l.) proteins using improved procedures for high resolution 2-de, which were adapted for the separation of rice lamina proteins. validation of this system was achieved by measuring the effects of sample preparation and biological variation on the coefficient ... | 2006 | 16513811 |
| purification, crystallization and preliminary x-ray crystallographic analysis of rice lectin from oryza sativa. | lectins with sugar-binding specificity are widely distributed in higher plants and various other species. the expression of rice lectin from oryza sativa is up-regulated in the growing coleoptile when anaerobic stress persists. a rice lectin of molecular weight 15.2 kda has been crystallized using the hanging-drop vapour-diffusion method. from the diffraction of the lectin crystals at 1.93 a resolution, the unit cell belongs to space group p3(1), with unit-cell parameters a = 98.58, b = 98.58, c ... | 2006 | 16511272 |
| alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (oryza sativa l.) genotypes contrasting in chilling tolerance. | abiotic stress is a major limiting factor in crop production. physiological comparisons between contrasting abiotic stress-tolerant genotypes will improve understanding of stress-tolerant mechanisms. rice seedlings (s3 stage) of a chilling-tolerant (ct) genotype (ct6748-8-ca-17) and a chilling-sensitive (cs) genotype (iniap12) were subjected to abiotic stresses including chilling (13/12 degrees c), salt (100mm nacl), and osmotic (200mm mannitol). measures of physiological response to the stresse ... | 2007 | 16500726 |
| 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase is regulated for the accumulation of polysaccharide-linked hydroxycinnamoyl esters in rice (oryza sativa l.) internode cell walls. | polysaccharide-linked hydroxycinnamoyl esters (phes) over-accumulate in the internodes of a rice (oryza sativa l.) mutant, fukei 71 (f71). this accumulation is accompanied by over-expression of phenylalanine ammonialyase (pal). in this study, we show that only one member of the 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (dahps) family expresses in close correlation with pal. furthermore, substrate availability to dahps is promoted by down-regulating the expression of plastidic pyruvate ... | 2006 | 16496151 |
| ethanolic fermentation and anoxia tolerance in four rice cultivars. | the relationship between coleoptile elongation and ethanolic fermentation was investigated in rice (oryza sativa l.) coleoptiles of four cultivars subjected to a 48-h anoxic stress. the coleoptile elongation of all cultivars was suppressed by anoxic stress; however, the elongation of cvs yukihikari and nipponbare was much greater than that of cvs leulikelash and asahimochi. the stress did not significantly increase lactate dehydrogenase (ldh) activity or lactate concentration, but increased alco ... | 2007 | 16483690 |
| morphological and molecular characterization of different echinochloa spp. and oryza sativa populations. | echinochloa p. beauv. is an important genus because many of its species are weeds infesting most paddy fields, which can reduce the rice grain production by up to 80%. a controversy exists about the taxonomy of the genus due to the high level of morphological variations found in these species. cyhalofop-butyl, an aryloxyphenoxy-propionate herbicide, is used to control echinochloa spp. in paddy fields, although differences in susceptibility were found between different echinochloa species. e. col ... | 2006 | 16478232 |
| [a preliminary analysis of the function of the osrab5a gene in rice (oryza sativa l.)]. | osrab5a encodes a rab5 protein in rice, which belongs to the rab family of small gtp-binding protein superfamily. transgenic plants with posrab5a::beta-glucuronidase (gus) gene indicate that osrab5a is expressed in callus, root, stem, leaf, root base and spikelet (figs.1, 2h-1). using the transgenic arabidopsis with fused gene of gfp::osrab5a and gfp::osrab5aca and by using fm4-64 (the lipophilic styryl dye) staining, we have demonstrated that osrab5a is involved in vesicular transport and gfp-o ... | 2006 | 16477129 |
| photosynthetic light and co2 utilization and c4 traits of two novel super-rice hybrids. | characteristics of photosynthetic light and co2 use efficiency from seedling to heading stage, and c4 pathway enzyme activities in both flag leaves and lemma were compared between two newly developed super-rice hybrids (oryza sativa l.), liangyoupeijiu and hua-an 3, and a traditional rice hybrid, shanyou 63. at seedling and tillering stages, liangyoupeijiu and hua-an 3 had higher net photosynthetic rates (pn) and light saturated assimilation rates (asat) than did shanyou 63, at both normal (360 ... | 2006 | 16473657 |
| molecular characterization and differential expression of cytokinin-responsive type-a response regulators in rice (oryza sativa). | the response regulators represent the elements of bacterial two-component system and have been characterized from dicot plants like arabidopsis but little information is available on the monocots, including the cereal crops. the aim of this study was to characterize type-a response regulator genes from rice, and to investigate their expression in various organs as well as in response to different hormones, including cytokinin, and environmental stimuli. | 2006 | 16472405 |
| computational identification and systematic analysis of the acr gene family in oryza sativa. | based on sequence similarity search and domain detection, nine act domain repeat protein-coding genes (the "acr" genes) in rice were identified, which were mainly distributed on the chromosomes 2, 3, 4, and 8. an interpro database search indicated that four copies of the act domain linearly occupied the entire polypeptide. the first three act domains were linked by two different sequences. however, the fourth act domain was extremely close to act3. gene structure comparisons showed large differe ... | 2006 | 16455358 |
| chemical constituents of rice (oryza sativa) hulls and their herbicidal activity against duckweed (lemna paucicostata hegelm 381). | four new compounds, stigmastanol-3beta-p-glyceroxydihydrocoumaroate (1), stigmastanol-3beta-p-butanoxydihydrocoumaroate (2), lanast-7,9(11)-dien-3alpha,15alpha-diol-3alpha-d-glucofuranoside (3) and 1-phenyl-2-hydroxy-3,7-dimethyl-11-aldehydic-tetradecane-2-beta-d-glucopyranoside (4), along with several known compounds were isolated from the methanol extract of hulls of oryza sativa. the new structures were established by one- and two-dimensional nmr and in combination with ir, ei/ms, fab/ms and ... | 2015 | 16454475 |
| analysis of sialyltransferase-like proteins from oryza sativa. | sialic acids are widely distributed among living creatures, from bacteria to mammals, but it has been commonly accepted that they do not exist in plants. however, with the progress of genome analyses, putative gene homologs of animal sialyltransferases have been detected in the genome of some plants. in this study, we cloned three genes from oryza sativa (japanese rice) that encode sialyltransferase-like proteins, designated osstlp1, 2, and 3, and analyzed the enzymatic activity of the proteins. ... | 2006 | 16452316 |
| function and characterization of starch synthase i using mutants in rice. | four starch synthase i (ssi)-deficient rice (oryza sativa) mutant lines were generated using retrotransposon tos17 insertion. the mutants exhibited different levels of ssi activities and produced significantly lower amounts of ssi protein ranging from 0% to 20% of the wild type. the mutant endosperm amylopectin showed a decrease in chains with degree of polymerization (dp) 8 to 12 and an increase in chains with dp 6 to 7 and dp 16 to 19. the degree of change in amylopectin chain-length distribut ... | 2006 | 16443699 |
| the accumulation of deleterious mutations in rice genomes: a hypothesis on the cost of domestication. | the extent of molecular differentiation between domesticated animals or plants and their wild relatives is postulated to be small. the availability of the complete genome sequences of two subspecies of the asian rice, oryza sativa (indica and japonica) and their wild relatives have provided an unprecedented opportunity to study divergence following domestication. we observed significantly more amino acid substitutions during rice domestication than can be expected from a comparison among wild sp ... | 2006 | 16443304 |
| identification of differentially expressed genes in brown planthopper nilaparvata lugens (hemiptera: delphacidae) responding to host plant resistance. | the brown planthopper nilaparvata lugens stål is one of the major insect pests of rice oryza sativa l. the host resistance exhibits profound effects on growth, development and propagation of n. lugens. to investigate the molecular response of n. lugens to host resistance, a cdna-amplified fragment length polymorphism (cdna-aflp) technique was employed to identify the differentially expressed genes in the nymphs feeding on three rice varieties. of the 2,800 cdna bands analysed, 54 were up-regulat ... | 2006 | 16441905 |
| a segment of the apospory-specific genomic region is highly microsyntenic not only between the apomicts pennisetum squamulatum and buffelgrass, but also with a rice chromosome 11 centromeric-proximal genomic region. | bacterial artificial chromosome (bac) clones from apomicts pennisetum squamulatum and buffelgrass (cenchrus ciliaris), isolated with the apospory-specific genomic region (asgr) marker ugt197, were assembled into contigs that were extended by chromosome walking. gene-like sequences from contigs were identified by shotgun sequencing and blast searches, and used to isolate orthologous rice contigs. additional gene-like sequences in the apomicts' contigs were identified by bioinformatics using fully ... | 2006 | 16415213 |
| characterization of a rice (oryza sativa l.) gene encoding a temperature-dependent chloroplast omega-3 fatty acid desaturase. | a cdna, designated osfad8, encoding a chloroplast omega-3 fatty acid desaturase responsible for trienoic fatty acid formation, was isolated from the leaves of oryza sativa l. by rt-pcr. southern blot hybridization indicated that a small gene family composed of two copies or closely linked genes exists. rna in situ hybridization showed that the accumulation of osfad8 mrna was abundant in leaves but hardly detectable in roots. the osfad8 transcript level in leaves was much higher at 15 degrees c t ... | 2006 | 16406238 |
| oryzabase. an integrated biological and genome information database for rice. | the aim of oryzabase is to create a comprehensive view of rice (oryza sativa) as a model monocot plant by integrating biological data with molecular genomic information (http://www.shigen.nig.ac.jp/rice/oryzabase/top/top.jsp). the database contains information about rice development and anatomy, rice mutants, and genetic resources, especially for wild varieties of rice. the anatomical description of rice development is unique and is the first known representation for rice. developmental and anat ... | 2006 | 16403737 |
| cadmium pollution in paddy soil as affected by different rice (oryza sativa l.) cultivars. | | 2005 | 16400554 |
| seasonal changes in temperature dependence of photosynthetic rate in rice under a free-air co(2) enrichment. | influences of rising global co(2) concentration and temperature on plant growth and ecosystem function have become major concerns, but how photosynthesis changes with co(2) and temperature in the field is poorly understood. therefore, studies were made of the effect of elevated co(2) on temperature dependence of photosynthetic rates in rice (oryza sativa) grown in a paddy field, in relation to seasons in two years. | 2006 | 16399793 |
| global patterns of gene expression in the aleurone of wild-type and dwarf1 mutant rice. | the cereal aleurone layer is a model system for studying the regulation of transcription by gibberellin (ga) and abscisic acid (aba). ga stimulates and aba prevents the transcription of genes for alpha-amylases and other secreted hydrolytic enzymes, but how ga and aba affect the transcription of other genes is largely unknown. we characterized gene expression in rice (oryza sativa) aleurone using a half-genome rice microarray. of the 23,000 probe sets on the chip, approximately 11,000 hybridized ... | 2006 | 16384900 |
| the rice annotation project database (rap-db): hub for oryza sativa ssp. japonica genome information. | with the completion of the rice genome sequencing, a standardized annotation is necessary so that the information from the genome sequence can be fully utilized in understanding the biology of rice and other cereal crops. an annotation jamboree was held in japan with the aim of annotating and manually curating all the genes in the rice genome. here we present the rice annotation project database (rap-db), which has been developed to provide access to the annotation data. the rap-db has two diffe ... | 2006 | 16381971 |
| gramene: a bird's eye view of cereal genomes. | rice, maize, sorghum, wheat, barley and the other major crop grasses from the family poaceae (gramineae) are mankind's most important source of calories and contribute tens of billions of dollars annually to the world economy (fao 1999, http://www.fao.org; usda 1997, http://www.usda.gov). continued improvement of poaceae crops is necessary in order to continue to feed an ever-growing world population. however, of the major crop grasses, only rice (oryza sativa), with a compact genome of approxim ... | 2006 | 16381966 |
| a rice glutamate receptor-like gene is critical for the division and survival of individual cells in the root apical meristem. | glu receptors are known to function as glu-activated ion channels that mediate mostly excitatory neurotransmission in animals. glu receptor-like genes have also been reported in higher plants, although their function is largely unknown. we have identified a rice (oryza sativa) glu receptor-like gene, designated glr3.1, in which mutation by t-dna insertion caused a short-root mutant phenotype. histology and dna synthesis analyses revealed that the mutant root meristematic activity is distorted an ... | 2006 | 16377757 |
| genome-wide transcription analyses in rice using tiling microarrays. | sequencing and computational annotation revealed several features, including high gene numbers, unusual composition of the predicted genes and a large number of genes lacking homology to known genes, that distinguish the rice (oryza sativa) genome from that of other fully sequenced model species. we report here a full-genome transcription analysis of the indica rice subspecies using high-density oligonucleotide tiling microarrays. our results provided expression data support for the existence of ... | 2006 | 16369532 |
| an integrated map of oryza sativa l. chromosome 5. | the developments of molecular marker-based genetic linkage maps are now routine. physical maps based on contigs of large insert genomic clones have been established in several plant species. however, integration of genetic, physical, and cytological maps is still a challenge for most plant species. here we present an integrated map of rice (oryza sativa l.) chromosome 5, developed by fluorescence in situ hybridization mapping of 18 bacterial artificial chromosome (bac) clones or pi-derived artif ... | 2006 | 16365756 |
| [changes in some defensive enzyme activity induced by the piercing-sucking of brown planthopper in rice]. | the effects of brown planthopper on the activities of some key enzymes involved in defense response and the h(2)o(2) content were investigated in rice. from the fact that after piercing-sucking of brown planthopper, malondialdehyde (mda) content of rice plant was increased rapidly (fig.3), one can conclude that it induced lipid peroxidation; lipoxygenase (lox), hydroperoxide lyase (hpl) and phenylalanine ammonia-lyase (pal) activities were increased significantly in plants of resistant and susce ... | 2005 | 16361793 |
| [genetic analysis and mapping of the rice leafy-hull mutant oslh]. | oslh (lh=leafy hull), in the japonica cultivar 9522 background, a mutant of oryza sativa l. spp. japonica cv. 9522 identified from an m(2) population, was mutagenized by irradiation with (60)co gamma-ray. the oslh mutant plants flowered about 15 days later than the wild-type plants (fig.1e). the paleas, lemmas and lodicules of the flowers of oslh mutant were transformed into leaf-like structures (fig.1b, d). genetic analysis of the f(2) progeny from a cross between the oslh mutant and wild-type ... | 2005 | 16361786 |
| study of rice pollen grains by multispectral imaging microscopy. | cellular images obtained by light microscopy have been analyzed qualitatively, but there is still a lack of quantitative information about the variations in cellular metabolism of selective substances. a new approach using the multispectral imaging microscope (mim) to observe rice (oryza sativa l.) pollen grains is reported. a liquid crystal tunable filter device was used for wavelength selection from 400 to 720 nm and a cooled two-dimensional monochrome charge coupled device for image detection ... | 2005 | 16358284 |
| mapping segregation distortion loci and quantitative trait loci for spikelet sterility in rice ( oryza sativa l.). | markers with segregation ratio distortion are commonly observed in data sets used for quantitative trait locus (qtl) mapping. in this study, a multipoint method of maximum likelihood (ml) was newly developed to estimate the positions and effects of the segregation distortion loci (sdls) in two f2 populations of rice (oryza sativa l.), i.e. taichung65/bhadua (tb; japonica-indica cross) and cpslo17/w207-2 (cw; japonica-japonica). of the four parents, w207-2 and bhadua were found to be spikelet sem ... | 2005 | 16356283 |
| the oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert bac libraries that represent the 10 genome types of the genus oryza. | rice (oryza sativa l.) is the most important food crop in the world and a model system for plant biology. with the completion of a finished genome sequence we must now functionally characterize the rice genome by a variety of methods, including comparative genomic analysis between cereal species and within the genus oryza. oryza contains two cultivated and 22 wild species that represent 10 distinct genome types. the wild species contain an essentially untapped reservoir of agriculturally importa ... | 2006 | 16344555 |
| qtls influencing panicle size detected in two reciprocal introgressive line (il) populations in rice (oryza sativa l.). | two sets of reciprocal introgression line (il) populations, i.e., ils with lemont as recurrent parent (il_lt) and ils with teqing as recurrent parent (il_tq), were developed and evaluated for traits representing panicle size, including primary branch number (pbn), secondary branch number (sbn), and spikelet number per panicle (snp). together with the regression to recurrent parent by advanced backcross, transgressive segregations were observed for all traits. correlation and regression analysis ... | 2006 | 16331475 |
| post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene. | the purpose of this study was to test the hypothesis that the interaction between abscisic acid (aba) and ethylene may be involved in mediating the post-anthesis development of spikelets in rice (oryza sativa l.). two rice genotypes were field-grown, and the changes of aba, ethylene, and 1-aminocylopropane-1-carboxylic acid (acc) levels in spikelets during grain filling and their relationships with endosperm-division and grain-filling rates were investigated. the results showed that earlier-flow ... | 2006 | 16330527 |
| construction of suppression subtractive hybridization libraries and identification of brown planthopper-induced genes. | a suppression subtractive hybridization technique was used to screen for brown planthopper (bph)-inducible genes in rice (oryza sativa). cdnas from a bph-resistant rice line (b5) infested by bph were used as the tester population, and mixed cdnas from a bph-sensitive line (mh63) and a control (uninfested b5) as the driver population. after hybridizing and cloning, forward and reverse subtraction cdna libraries were obtained, containing 5700 clones. these clones were further analyzed by different ... | 2005 | 16323277 |
| mapping of qtls for ferrous iron toxicity tolerance in rice (oryza sativa l.). | ferrous iron toxicity is the main factor limiting the productivity of rice in gleyic paddy soils. in this study, an f2 and an equivalent f3 populations derived from a japonica/indica cross of rice, longza8503/ir64, were raised under iron-enriched solution cultures, and used to map qtls controlling ferrous iron toxicity tolerance. a genetic linkage map consisting of 101 ssr markers was constructed to determine the position and nature of quantitative trait loci (qtls) affecting fe2+ toxicity toler ... | 2005 | 16318281 |
| fine mapping of f5-du, a gene conferring wide-compatibility for pollen fertility in inter-subspecific hybrids of rice (oryza sativa l.). | wide-compatibility varieties (wcvs), comprising a special class of rice germplasm, are able to produce fertile hybrids when crossed with both indica and japonica varieties. dular, a landrace variety from india, has both a wide spectrum and high level of wide-compatibility when crossed with a range of indica and japonica varieties. in previous studies, an allele at the f5 locus from dular (f5-du) was identified as a neutral allele conferring wide-compatibility with a large effect on both pollen a ... | 2006 | 16317572 |
| comparative proteomic analysis provides new insights into chilling stress responses in rice. | low temperature is one of the major abiotic stresses limiting the productivity and the geographical distribution of many important crops. to gain a better understanding of chilling stress responses in rice (oryza sativa l. cv. nipponbare), we carried out a comparative proteomic analysis. three-week-old rice seedlings were treated at 6 degrees c for 6 or 24 h and then recovered for 24 h. chilling treatment resulted in stress phenotypes of rolling leaves, increased relative electrolyte leakage, an ... | 2006 | 16316980 |
| embolized conduits of rice (oryza sativa, poaceae) refill despite negative xylem pressure. | embolism reversal in rice plants was studied by testing the plant's ability to refill embolized conduits while xylem pressures were substantially negative. intact, potted plants were water-stressed to a xylem pressure of -1.88 ± 0.1 mpa and a 66.3 ± 3.8% loss of xylem conductivity (plc) by cavitation. stressed plants were carefully rewatered, allowing xylem pressure to rise, but not above the theoretical threshold of c. -0.15 mpa for embolism collapse. despite xylem pressures being more negative ... | 2005 | 21646115 |
| effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance. | rice (oryza sativa l.) roots were fed with l-ascorbic acid (asa) and its putative precursors to observe asa and oxalate concentrations and the resistance of rice to chilling, water stress, and al toxicity. asa concentration was significantly enhanced in both shoots and roots of rice seedlings by feeding with d-glucose or l-galactono-gamma-lactone. asa or l-galactono-gamma-lactone treatment increased accumulation of oxalate mainly in soluble form, while these treatments decreased electrolyte leak ... | 2016 | 16310370 |
| a novel function of abscisic acid in the regulation of rice (oryza sativa l.) root growth and development. | plant roots retain developmental plasticity and respond to environmental stresses or exogenous plant growth regulators by undergoing profound morphological and physiological alteration. in this study, we investigated the effects of exogenous aba on root growth and development in taichung native 1 (tn1) rice. exogenous application of 10 microm aba leads to swelling, root hair formation and initiation of lateral root primodia in the tips of young, seminal rice roots. cortex cells increased in size ... | 2006 | 16299003 |
| a loss-of-function mutation in atysl1 reveals its role in iron and nicotianamine seed loading. | the arabidopsis yellow stripe 1-like (ysl) proteins have been identified by homology with the maize (zea mays) yellow stripe 1 (ys1) transporter which is responsible for iron-phytosiderophore (ps) uptake by roots in response to iron shortage. although dicotyledonous plants do not synthesize ps, they do synthesize the ps precursor nicotianamine, a strong metal chelator essential for maintenance of iron homeostasis and copper translocation. furthermore, zmys1 and the rice (oryza sativa) protein os ... | 2005 | 16297069 |
| the immediate-early ethylene response gene osard1 encodes an acireductone dioxygenase involved in recycling of the ethylene precursor s-adenosylmethionine. | methylthioadenosine (mta) is formed as a by-product of ethylene biosynthesis from s-adenosyl-l-methionine (adomet). the methionine cycle regenerates adomet from mta. in two independent differential screens for submergence-induced genes and for 1-aminocyclopropane-1-carboxylic acid (acc)-induced genes from deepwater rice (oryza sativa l.) we identified an acireductone dioxygenase (ard). osard1 is a metal-binding protein that belongs to the cupin superfamily. acireductone dioxygenases are unique p ... | 2005 | 16297065 |
| distinct and cooperative functions of phytochromes a, b, and c in the control of deetiolation and flowering in rice. | we have isolated phytochrome b (phyb) and phyc mutants from rice (oryza sativa) and have produced all combinations of double mutants. seedlings of phyb and phyb phyc mutants exhibited a partial loss of sensitivity to continuous red light (rc) but still showed significant deetiolation responses. the responses to rc were completely canceled in phya phyb double mutants. these results indicate that phya and phyb act in a highly redundant manner to control deetiolation under rc. under continuous far- ... | 2005 | 16278346 |
| uptake of sodium in protoplasts of salt-sensitive and salt-tolerant cultivars of rice, oryza sativa l. determined by the fluorescent dye sbfi. | in this study, the uptake of na+ into the cytosol of rice (oryza sativa l. cvs pokkali and brri dhan29) protoplasts was measured using the acetoxy methyl ester of the fluorescent sodium-binding benzofuran isopthalate, sbfi-am, and fluorescence microscopy. by means of inhibitor analyses the mechanisms for uptake and sequestration of na+ in the salt-sensitive indica rice cv. brri dhan29 and in the salt-tolerant indica rice cv. pokkali were detected. less na+ was taken up into the cytosol of pokkal ... | 2005 | 16275670 |
| a fluorescent antibiotic resistance marker for rapid production of transgenic rice plants. | blasticidin s (bs) is an aminoacylnucleoside antibiotic used for the control of rice blast disease. to establish a new cereal transformation system, we constructed a visual marker gene designated gfbsd, encoding an enhanced green fluorescent protein (egfp) fused to the n-terminus of bs deaminase (bsd). it was cloned into a monocot expression vector and introduced into rice (oryza sativa l. cv. nipponbare) calluses by microprojectile bombardment. three to five weeks after the bombardment, multice ... | 2006 | 16271791 |
| low temperature treatment at the young microspore stage induces protein changes in rice anthers. | male reproductive development in rice is very sensitive to various forms of environmental stresses including low temperature. a few days of cold treatment (<20 degrees c) at the young microspore stage induce severe pollen sterility and thus large grain yield reductions. to investigate this phenomenon, anther proteins at the early stages of microspore development, with or without cold treatment at 12 degrees c, were extracted, separated by two-dimensional gel electrophoresis, and compared. the co ... | 2006 | 16263700 |
| [root activity and nitrogen assimilation of rice (oryza sativa) under free-air co2 enrichment]. | with free-air co2 enrichment(face) technique, this paper studied the root activity and amino acid(aa) synthesis of rice (oryza sativa )at low n(ln, 150 kgn x hm(-2)) and normal n(nn, 250 kgn x hm(-2)) under ambient air and elevated atmospheric co2 (ambient + 200 micromol x mol(-1)). under elevated co2, the xylem exudates per hill changed little, while the xylem exudates per stem declined by 1.4% - 21.7% as the result of greater tiller numbers. at tillering and heading stages, elevated co2 increa ... | 2005 | 16262063 |
| a fine physical map of the rice chromosome 5. | a fine physical map of the rice (oryza sativa spp. japonica var. nipponbare) chromosome 5 with bacterial artificial chromosome (bac) and pi-derived artificial chromosome (pac) clones was constructed through integration of 280 sequenced bac/pac clones and 232 sequence tagged site/expressed sequence tag markers with the use of fingerprinted contig data of the nipponbare genome. this map consists of five contigs covering 99% of the estimated chromosome size (30.08 mb). the four physical gaps were e ... | 2005 | 16261349 |
| biochemical properties and localization of the beta-expansin osexpb3 in rice (oryza sativa l.). | alpha-expansins are bound to the cell wall of plants and can be solubilized with an extraction buffer containing 1 m nacl. localization of alpha-expansins in the cell wall was confirmed by immunogold labeling and electron microscopy. the subcellular localization of vegetative beta-expansins has not yet been studied. using antibodies specific for osexpb3, a vegetative beta-expansin of rice (oryza sativa l.), we found that osexpb3 is tightly bound to the cell wall and, unlike alpha-expansins, cann ... | 2005 | 16258250 |
| [study on early stability by issr markers in rice]. | in the f2 population crossed from two early stability rice (oryza sativa l.) with four cultivars, eight uniform strains were recorded. genetic analysis showed that both uniform strains with uniform agronomic characteristics and segregated strains segregating in mendelian manner were observed in f2 population of the same combination. four types of marker-bands were obtained after the f2 uniform strains were marked by 26 issr primers:t ype i, maternal marker bands present, and paternal marker band ... | 2005 | 16252703 |
| qtl for yield and its components responded to elevated co2 in rice (oryza sativa l.). | face (free air carbon dioxide enrichment) technology may provide a means by which the environment around growing plants can be modified to realistically simulate the concentration of atmospheric co2 in the future. the plant growth and its yield of plant species can be enhanced under face. identification of genomic regions influencing the response of yield and its components to elevated co2 will be useful for understanding the genetics of active response to changed co2 environment and developing ... | 2005 | 16252702 |
| efficiency of rice bran for the removal of selected organics from water: kinetic and thermodynamic investigations. | the sorption efficiency of indigenous rice (oryza sativa) bran for the removal of organics, that is, benzene, toluene, ethylbenzene, and cumene (btec), from aqueous solutions has been studied. the sorption of btec by rice bran is observed over a wide ph range of 1-10, indicating its high applicability to remove these organics from various industrial effluents. rice bran effectively adsorbs btec of 10 microg ml(-1) sorbate concentration from water at temperatures of 283-323 +/- 2 k. the effect of ... | 2005 | 16248568 |
| salt-responsive genes in rice revealed by cdna microarray analysis. | we used cdna microarrays containing approximately 9,000 unigenes to identify 486 salt responsive expressed sequence tags (ests) (representing approximately 450 unigenes) in shoots of the highly salt-tolerant rice variety, nona bokra (oryza sativa l. ssp. indica pv. nona). some of the genes identified in this study had previously been associated with salt stress. however the majority were novel, indicating that there is a great number of genes that are induced by salt exposure. analysis of the sa ... | 2005 | 16246269 |
| crystal structure of putative n-acetyl-gamma-glutamyl-phosphate reductase (ak071544) from rice (oryza sativa). | | 2005 | 16240442 |
| high-resolution mapping of a new brown planthopper (bph) resistance gene, bph18(t), and marker-assisted selection for bph resistance in rice (oryza sativa l.). | brown planthopper (bph) is a destructive insect pest of rice in asia. identification and the incorporation of new bph resistance genes into modern rice cultivars are important breeding strategies to control the damage caused by new biotypes of bph. in this study, a major resistance gene, bph18(t), has been identified in an introgression line (ir65482-7-216-1-2) that has inherited the gene from the wild species oryza australiensis. genetic analysis revealed the dominant nature of the bph18(t) gen ... | 2006 | 16240104 |
| purification and characterization of rice alpha-glucosidase, a key enzyme for alcohol fermentation of rice polish. | alpha-glucosidase, a key enzyme for nuka-sake brewing, was purified from oryza sativa cv. yamadanishiki, which is widely used for sake brewing. the molecular weight of the purified enzyme was 95 kda. the optimum ph and temperature were 4.5 and 55 degrees c, respectively. the substrate specificity differed from that of oryza sativa cv. shinsetsu, which is a variety of rice consumed as a cereal. the extraction of alpha-glucosidase from the rice was stimulated by lactic acid, which suggests that la ... | 2003 | 16233375 |
| enhanced regeneration of rice (oryza sativa l.) embryogenic callus by light irradiation in growth phase. | the effect of light irradiation on the growth and regeneration of embryogenic rice calli was investigated. rice calli grew slightly better in the light than in the dark during the growth stage. when calli subcultured under light irradiation were transferred into a liquid regeneration culture system, the number of plantlets with shoots regenerated from calli subcultured in the light was higher than in the case of those subcultured in the dark, whereas there was no obvious difference between the l ... | 2001 | 16232998 |
| in situ regeneration of rice (oryza sativa l.) callus immobilized in polyurethane foam. | an in situ regeneration system for rice calli comprised of a callus growth stage and two regeneration stages was developed. after the first stage of regeneration, the medium is changed and the calli are immobilized in polyurethane foam supports, in each of which 3-5 regenerated plantlets develop from the immobilized calli during the second stage. while no significant change in callus size was observed during the first stage of regeneration, in the second stage callus enlargement and shoot regene ... | 2001 | 16232950 |
| development of a bioreactor suitable for embryogenic rice callus culture. | embryogenic rice calli induced from mature rice (oryza sativa l., sasanishiki) were cultured in a jarfermentor with disk turbine impellers, an air-lift reactor, and a turbine-blade reactor (tbr(r)), and the effects of agitation were investigated. in all cases, the growth inhibition was observed, though a slightly improved regeneration frequency was obtained in the tbr. to overcome the growth inhibition, small cubes of polyurethane foam were used as immobilization supports in the tbr culture. sup ... | 1999 | 16232535 |
| influence of different oryza cultivars on expression of nifh gene pools in roots of rice. | cultivation-independent studies suggest that roots of rice (oryza sativa) are colonized by a diverse community of nitrogen-fixing bacteria. here we report for the first time mrna-based profiling of nitrogenase (nifh) genes, to study the impact of lowland-rice genotypes at the cultivar level on the functional diversity of root-associated diazotrophs. root rna extracts from all plants contained nifh mrna at levels detectable by reverse transcription polymerase chain reaction (rt-pcr). terminal res ... | 2005 | 16232287 |
| fish analysis of pachytene chromosome and dna fiber of telomere sequence in rice (oryza sativa l. indica). | telomere sequences were analyzed by using pachytene chromosome and extended dna fiber fish in rice (oryza, sativa ssp. indica cv. -guangluai no.4). pachytene fish results showed that most of chromosome ends possess the telomere tandem repeats, but the signals on different chromosomes were not the same in intensity. fiber fish results indicated that the longest string of beads was 6.55 microm, while the shortest one was 1.82 microm long,which were equal to 16.44 and 4.56 kb correspondingly based ... | 2005 | 16231738 |
| [developing single segment substitution lines (sssls) in rice (oryza sativa l.) using advanced backcrosses and mas]. | a novel population consisted of 86 single segment substitution lines (sssls) were developed from advanced backcrosses between the recipient, huajingxian 74 and six donors by microsatellite marker-assisted selection (mas). fifty-two sssls were selected in bc3f2, and 34 others were selected in bc3f3. every sssl contains a single chromosome segment introgressed from one donor on the genetic background of huajingxian 74. the substituted segments in sssls were distributed on 12 rice chromosomes. the ... | 2005 | 16231737 |
| [qtl analysis for traits associated with photosynthetic functions in rice (oryza sativa l.)]. | a mapping population of 81 f11 lines (recombinant inbred lines, rils), derived from a cross between a japonica variety kinmaze and an indica variety dv85 by single-seed descent method, was used to detect quantitative trait loci (qtl) for traits associated with photosynthetic functions. total leaf nitrogen content (tln), chlorophyll a/b ratio (chl. a:b) and chlorophyll content (chl) were measured in leaves of rice (oryza sativa l.) at the 7th day after heading. a total of six putative qtls were d ... | 2005 | 16231736 |
| grain yield responses to moisture regimes in a rice population: association among traits and genetic markers. | drought is a major constraint to rice (oryza sativa l.) production in rainfed and poorly irrigated environments. identifying genomic regions influencing the response of yield and its components to water deficits will aid our understanding of the genetic mechanism of drought tolerance (dt) of rice and the development of dt varieties. grain yield (gy) and its components of a recombinant inbred population developed from a lowland rice and an upland rice were investigated under different water level ... | 2005 | 16231161 |
| microarray analysis reveals similarities and variations in genetic programs controlling pollination/fertilization and stress responses in rice (oryza sativa l.). | previously, we identified 253 cdnas that are regulated by pollination/fertilization in rice by using a 10k cdna microarray. in addition, many of them also appeared to be involved in drought and wounding responses. to investigate this relationship, we obtained their expression profiles after dehydration and wounding treatments in this study. venn diagram analysis indicated that 53.8% (136/253) and 21% (57/253) of the pollination/fertilization-related genes are indeed regulated by dehydration and ... | 2005 | 16217609 |
| the plant architecture of rice (oryza sativa). | plant architecture, a collection of the important agronomic traits that determine grain production in rice, is mainly affected by factors including tillering, plant height and panicle morphology. recently, significant progress has been made in isolating and collecting of mutants that are defective in rice plant architecture. although our understanding of the molecular mechanisms that control rice tillering, panicle development and plant height are still limited, new findings have begun to emerge ... | 2005 | 16217603 |
| genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (oryza sativa l.). | tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (fg) research since the rice genome was completely sequenced. to facilitate fg research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ils) in three elite rice genetic backgrounds for a wide range of complex traits, including resistances/tolerances to many biotic and abiotic stresses, morpho-agronomic traits, physiological traits, ... | 2005 | 16217600 |
| cloning and characterization of cdna for the oryza sativa phosphate transporter. | a putative high-affinity phosphate (pi) transporter gene in rice (oryza sativa), oslpt1, was isolated by rt-pcr from the leaves of the plants. the 1635-bp nucleotide sequence of oslpt1 spans an open reading frame encoding a polypeptide of 535 amino acids with sequence similarity to phosphate transporters from other plant species. southern blot analysis showed that the oslpt1 gene might be present in three transcripts in the rice genome. rt-pcr analysis demonstrated the expression of oslpt1 in bo ... | 2005 | 16217552 |
| cloning and characterization of osorc2, a new member of rice origin recognition complex. | in eukaryotic cells, the origin recognition complex (orc) governs the initiation site of dna replication and formation of the prereplication complex. the isolation, characterization and tissue-specific expression of a putative orc subunit 2 (osorc2) in oryza sativa is described here. a novel cdna fragment encoding rice orc2 was isolated by screening the subtractive library, which had a higher expression level in inflorescence meristem than in shoot apical meristem. the full-length cdna of rice o ... | 2005 | 16215849 |
| metabolic conversion of 24-methyl-delta25-cholesterol to 24-methylcholesterol in higher plants. | feeding of chemically synthesized [27-13c]codisterol ([27-13c]2), [27-13c]24-epicodisterol ([27-13c]3), [23,24-2h2]codisterol ([23,24-2h2]2), and [26,27-2h6]24-methyldesmosterol ([26,27-2h6]8) to oryza sativa cell cultures, followed by ms and nmr analysis of the biosynthesized dihydrobrassicasterol (9)/campesterol (10), revealed that both (24r)- and (24s)-epimers of 24-methyl-delta25-cholesterol (2/3) were converted to 9 and 10 via the common intermediate 24-methyldesmosterol (8). | 2006 | 16213729 |
| anaconda, a new class of transposon belonging to the mu superfamily, has diversified by acquiring host genes during rice evolution. | a new type of transposon, named anaconda (anac) has been found in rice (oryza sativa). in this paper, we demonstrate that anaconda elements have diversified by acquisition of host cellular genes, amplification of the elements, and substitution and deletion of short segments. we identified four anaconda elements in studies of rice alternative oxidase (aox) genes, and subsequently isolated an additional 23 elements based on the identity of their terminal inverted repeats (tirs). the anaconda eleme ... | 2005 | 16208489 |
| [qtls mapping and genetic analysis of tiller angle in rice (oryza sativa l.)]. | tiller angle is one of the most important morphological characters that has a significant effect on the formation of rice high-yield population. the tiller angles were measured in a japonica/indica ril population with 71 lines and a genome-wide chromosome segment substitution line (cssl) population with 65 lines at two experimental sites. a transgressive segregation was observed in both populations. qtl analysis of tiller angle was conducted based on the saturated rflp marker linkage map and the ... | 2005 | 16201239 |
| random sheared fosmid library as a new genomic tool to accelerate complete finishing of rice (oryza sativa spp. nipponbare) genome sequence: sequencing of gap-specific fosmid clones uncovers new euchromatic portions of the genome. | the international rice genome sequencing project has recently announced the high-quality finished sequence that covers nearly 95% of the japonica rice genome representing 370 mbp. nevertheless, the current physical map of japonica rice contains 62 physical gaps corresponding to approximately 5% of the genome, that have not been identified/represented in the comprehensive array of publicly available bac, pac and other genomic library resources. without finishing these gaps, it is impossible to id ... | 2005 | 16200416 |
| functional analysis of dna sequences controlling the expression of the rice oscdpk2 gene. | plant calcium-dependent protein kinases (cdpks) are involved in calcium-mediated signal transduction pathways. their expression is finely tuned in different tissues and in response to specific signals, but the mechanism of such a regulation is still largely unknown. oscdpk2 gene expression is modulated in vivo during rice (oryza sativa l.) flower development and is downregulated by white light in leaves. in order to identify oscdpk2 regulatory sequences, we amplified and cloned both the 5' and 3 ... | 2006 | 16200411 |
| structure and expression analysis of early auxin-responsive aux/iaa gene family in rice (oryza sativa). | auxin exerts pleiotropic effects on plant growth and development by regulating the expression of early auxin-responsive genes of auxin/indoleacetic acid (aux/iaa), small auxin-up rna, and gh3 classes. these genes have been studied extensively in dicots like soybean and arabidopsis. we had earlier characterized a cdna of the first monocot member of aux/iaa family from rice. the achievement of the large scale rice genome sequencing combined with the availability of full-length cdna sequences from ... | 2006 | 16200395 |
| a relationship between tolerance to dehydration of rice cell lines and ability for aba synthesis under stress. | plant dehydration is commonly caused by some adverse environmental conditions such as salinity, drought and freezing. as the plant hormone abscisic acid (aba) is involved in responses to water stress, we studied its putative relationship with the degree of tolerance to these abiotic stresses. for this purpose we used cell lines that had been established from mature embryos of rice (oryza sativa l, cvs. bahia and bomba), and selected by their high (l-t) or low (l-s) levels of tolerance to each ty ... | 2005 | 16198586 |
| accumulation and distribution of polycyclic aromatic hydrocarbons in rice (oryza sativa). | various tissues of rice plants were sampled from a pah contaminated site in tianjin, china at different growth stages of the ripening period and analyzed for pahs. pahs were much higher in roots than in the exposed tissues. grains and internodes accumulated much smaller amounts of pahs than leaves, hulls or ear axes. no specific gradient trends along roots, stem, ear axes, and grains were observed, suggesting that systematic translocation among them is unlikely. over the ripening period, pah con ... | 2006 | 16198033 |
| rice (oryza sativa) response to drift rates of glyphosate. | greenhouse and field studies were conducted to investigate response of two rice varieties, priscilla and cocodrie, to sub-lethal rates of glyphosate in terms of injury, shikimate accumulation and yield. in the greenhouse, more shikimate accumulated in cocodrie than priscilla at comparable glyphosate rates applied to plants at the three-leaf stage. in field studies, glyphosate was applied to both varieties when they were 74-cm tall and in the internode separation growth stage. visual injury, plan ... | 2005 | 16189844 |
| active site residues and amino acid specificity of the ubiquitin carrier protein-binding ring-h2 finger domain. | el5 is a rice ubiquitin-protein isopeptide ligase (e3) containing a ring-h2 finger domain that interacts with oryza sativa (os) ubc5b, a rice ubiquitin carrier protein. we introduced point mutations into the el5 ring-h2 finger so that residues that functionally interact with osubc5b could be identified when assayed for ubiquitination activity in vitro. the residue positions were selected based on the results of an el5 ring-h2 finger/osubc5b nmr titration experiment. these ring-h2 finger residues ... | 2005 | 16186120 |
| comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. | rice (oryza sativa), a salt-sensitive species, has considerable genetic variation for salt tolerance within the cultivated gene pool. two indica rice genotypes, fl478, a recombinant inbred line derived from a population developed for salinity tolerance studies, and ir29, the sensitive parent of the population, were selected for this study. we used the affymetrix rice genome array containing 55,515 probe sets to explore the transcriptome of the salt-tolerant and salt-sensitive genotypes under con ... | 2005 | 16183841 |
| delimitation of the rice wide compatibility gene s5 ( n ) to a 40-kb dna fragment. | wide compatibility varieties (wcvs) are a special class of rice (oryza sativa l.) germplasm that produces hybrids with normal pollen and spikelet fertility when crossed with both indica and japonica subspecies. the wide compatibility gene s5 ( n ) has been used extensively in inter-subspecific hybrid breeding programs. we previously mapped the s5 locus to a 2.2-cm genomic region between rm253 and r2349 on chromosome 6, using a population of 356 f(1) plants derived from the three-way cross 02428/ ... | 2005 | 16177904 |
| concentration and release level of momilactone b in the seedlings of eight rice cultivars. | the release levels of a growth inhibitor, momilactone b, from rice (oryza sativa l.) seedlings of eight cultivars were compared with the endogenous concentrations of momilactone b in their seedlings. all rice cultivars contained momilactone b in the seedlings, and their concentrations differed between the cultivars. momilactone b was also found in all culture solutions in which these rice seedlings were grown, and the concentrations differed between the cultivars. the momilactone b concentration ... | 2005 | 16173457 |
| rna silencing of single and multiple members in a gene family of rice. | rna silencing with inverted repeat (ir) constructs has been used to suppress gene expression in various organisms. however, the transitive rna-silencing effect described in plants may preclude the use of rna silencing for a gene family. here, we show that, in rice (oryza sativa), transitive rna silencing (spreading of double-stranded rna along the target mrna) occurred with the green fluorescent protein transgene but not with the endogenous phytoene desaturase gene. we fused ir copies of unique ... | 2005 | 16172097 |
| epidermal cell death in rice is regulated by ethylene, gibberellin, and abscisic acid. | programmed cell death (pcd) of epidermal cells that cover adventitious root primordia in deepwater rice (oryza sativa) is induced by submergence. early suicide of epidermal cells may prevent injury to the growing root that emerges under flooding conditions. induction of pcd is dependent on ethylene signaling and is further promoted by gibberellin (ga). ethylene and ga act in a synergistic manner, indicating converging signaling pathways. treatment of plants with ga alone did not promote pcd. tre ... | 2005 | 16169967 |
| identification and characterization of a novel water-deficit-suppressed gene osard encoding an aci-reductone-dioxygenase-like protein in rice. | the aci-reductone dioxygenase (ard) family common to bacteria, plants and animals is involved in the methionine salvage pathway. a water-deficit-suppressed gene, osard encoding an aci-reductone-dioxygenase-like protein, was identified from rice (oryza sativa l.). northern blot and reverse transcriptase-polymerase chain reaction (rt-pcr) analysis revealed that the osard expression is regulated by abiotic stresses and phytohormones. osard was mainly expressed in roots under flood conditions. it wa ... | 2005 | 16169685 |
| interactions between ethylene, gibberellin and abscisic acid regulate emergence and growth rate of adventitious roots in deepwater rice. | growth of adventitious roots is induced in deepwater rice (oryza sativa l.) when plants become submerged. ethylene which accumulates in flooded plant parts is responsible for root growth induction. gibberellin (ga) is ineffective on its own but acts in a synergistic manner together with ethylene to promote the number of penetrating roots and the growth rate of emerged roots. studies with the ga biosynthesis inhibitor paclobutrazol revealed that root emergence was dependent on ga activity. abscis ... | 2006 | 16160845 |