| increase in cpd photolyase activity functions effectively to prevent growth inhibition caused by uvb radiation. | rice cultivars vary widely in their sensitivity to ultraviolet b (uvb) and this has been correlated with cyclobutane pyrimidine dimer (cpd) photolyase mutations that alter the structure/function of this photorepair enzyme. here, we tested whether cpd photolyase function determines the uvb sensitivity of rice (oryza sativa) by generating transgenic rice plants bearing the cpd photolyase gene of the uv-resistant rice cultivar sasanishiki in the sense orientation (s-b and s-c lines) or the antisens ... | 2007 | 17397507 |
| proteome and phosphoproteome differential expression under salinity stress in rice (oryza sativa) roots. | salinity stress is a major abiotic stress that limits agriculture productivity worldwide. rice is a model plant of monocotyledons, including cereal crops. studies have suggested a critical role of protein phosphorylation in salt stress response in plants. however, the phosphoproteome in rice, particularly under salinity stress, has not been well studied. here, we use pro-q diamond phosphoprotein stain to study rice phosphoproteome differential expression under salt stress. seventeen differential ... | 2007 | 17385905 |
| a multiresidue method for the analysis of pesticide residues in polished rice (oryza sativa l.) using accelerated solvent extraction and gas chromatography and confirmation by mass spectrometry. | an analytical procedure using accelerated solvent extraction and gas chromatography with an electron capture detector has been optimized to simultaneously determine the residue of two insecticides (diazinon and epn) and one fungicide (isoprothiolane) in polished rice and was confirmed by gc-mass spectrometry. several parameters, including temperature, pressure, solvent ratio, cell size and cell cycle, were thoroughly investigated to find the optimal extraction conditions. the average recoveries ... | 2007 | 17385804 |
| comprehensive expression profiling of rice grain filling-related genes under high temperature using dna microarray. | to elucidate the effect of high temperature on grain-filling metabolism, developing rice (oryza sativa) "nipponbare" caryopses were exposed to high temperature (33 degrees c/28 degrees c) or control temperature (25 degrees c/20 degrees c) during the milky stage. comprehensive gene screening by a 22-k dna microarray and differential hybridization, followed by expression analysis by semiquantitative reverse transcription-pcr, revealed that several starch synthesis-related genes, such as granule-bo ... | 2007 | 17384160 |
| saturated humidity accelerates lateral root development in rice (oryza sativa l.) seedlings by increasing phloem-based auxin transport. | auxin transport plays a significant role modifying plant growth and development in response to environmental signals such as light and gravity. however, the effect of humidity on auxin transport is rarely documented. it is shown here that the transport of labelled indole-3-acetic acid (iaa) from the shoot to the root is accelerated in rice (oryza sativa l. ssp. indica cv. ir8) seedlings grown under saturated humidity (sh-seedlings) compared with plants grown under normal humidity (nh-seedlings). ... | 2007 | 17383991 |
| analysis of the barley chromosome 2 region containing the six-rowed spike gene vrs1 reveals a breakdown of rice-barley micro collinearity by a transposition. | in cultivated barley (hordeum vulgare ssp. vulgare), six-rowed spikes produce three times as many seeds per spike as do two-rowed spikes. the determinant of this trait is the mendelian gene vrs1, located on chromosome 2h, which is syntenous with rice (oryza sativa) chromosomes 4 and 7. we exploited barley-rice micro-synteny to increase marker density in the vrs1 region as a prelude to its map-based cloning. the rice genomic sequence, covering a 980 kb contig, identified barley ests linked to vrs ... | 2007 | 17375281 |
| assessment of reclaimed wastewater irrigation impacts on water quality, soil, and rice cultivation in paddy fields. | the objective of this research was to monitor and assess the impact of reclaimed wastewater irrigation on water quality, soil, and rice cultivation by comparing the effects of various wastewater treatment levels on the growth and yield of rice. a randomized complete block design was used for the application methods of the wastewater effluents to paddy rice, with five treatments and six replications. the treatments were: control with groundwater irrigation (gw); irrigation with polluted water for ... | 2007 | 17365313 |
| cloning and sequence analysis of germin-like protein gene 2 promoter from oryza sativa l. ssp. indica. | germin and germin-like proteins (glps) are water soluble extracellular proteins reportedly expressed in response to some environmental and developmental signals. some enzymatic activities have also been associated with germin/glps. however, their role in overall metabolism has not been fully understood. significant insight into their function may also be gained by analysis of their promoter. during this study, about 1107 bp 5'region of osrglp2 gene was amplified, cloned and sequenced. the sequen ... | 2007 | 17364810 |
| molecular dissection of developmental behavior of tiller number and plant height and their relationship in rice (oryza sativa l.). | plant height and tiller number are two important characters related to yield in rice (oriza sativa l.). zhenshan97 x minghui63 recombinant inbred lines were employed to dissect the genetic basis of development of plant height and tiller number using conditional and unconditional composite interval mapping approaches. the traits were normally distributed with transgressive segregation in both directions. increasingly negative correlations were observed between tiller number and plant height at fi ... | 2006 | 17362360 |
| [nanoscale silicas in oryza sativa l. and their uv absorption]. | to reveal the unique microstructure of silica in rice and its absorption of ultra violet, wet digestion was chosen to isolate silica bodies from rice leaves and bract according to the fact that concentrated acid cannot destroy glass made of sio2. a mixed solution of sulfuric and nitric acids was applied to the leaves and bract of rice, respectively. after keeping the treated samples in 60-70 "c water bath for 30 hours and times of washing and sedimentation in water, pure silica bodies were obtai ... | 2006 | 17361739 |
| establishment of an in vitro fertilization system in rice (oryza sativa l.). | in vitro fertilization (ivf) systems using isolated male and female gametes have been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, as the female gametophytes of plants are deeply embedded within ovaries. in this study, a rice ivf system was established to take advantage of the abundant resources stemming from rice research for investigations into the mechanisms of fertilization and early embryogenesis. fusion ... | 2007 | 17361458 |
| bayesian association mapping of multiple quantitative trait loci and its application to the analysis of genetic variation among oryza sativa l. germplasms. | one way to use a crop germplasm collection directly to map qtls without using line-crossing experiments is the whole genome association mapping. a major problem with association mapping is the presence of population structure, which can lead to both false positives and failure to detect genuine associations (i.e., false negatives). particularly in highly selfing species such as asian cultivated rice, high levels of population structure are expected and therefore the efficiency of association map ... | 2007 | 17356864 |
| effect of 2,4-d, hydric stress and light on indica rice (oryza sativa) somatic embryogenesis. | with the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety cr-5272 (oryza sativa l.), two independent experiments were performed. the first experiment consisted in the effect of combination of three concentrations of the gelling agent phytagel (1.8. 2.4. and 3 gl(-1)) and four 2,4-d concentrations (2.26, 4.52. 6.78, and 9.05 microm) on the induction and subsequent regeneration of embryogenic calli. on the second e ... | 2015 | 17354447 |
| an expression atlas of rice mrnas and small rnas. | identification of all expressed transcripts in a sequenced genome is essential both for genome analysis and for realization of the goals of systems biology. we used the transcriptional profiling technology called 'massively parallel signature sequencing' to develop a comprehensive expression atlas of rice (oryza sativa cv nipponbare). we sequenced 46,971,553 mrna transcripts from 22 libraries, and 2,953,855 small rnas from 3 libraries. the data demonstrate widespread transcription throughout the ... | 2007 | 17351617 |
| assimilation of ammonium ions and reutilization of nitrogen in rice (oryza sativa l.). | a major source of inorganic nitrogen for rice plants grown in paddy soil is ammonium ions. the ammonium ions are actively taken up by the roots via ammonium transporters and subsequently assimilated into the amide residue of glutamine (gln) by the reaction of glutamine synthetase (gs) in the roots. the gln is converted into glutamate (glu), which is a central amino acid for the synthesis of a number of amino acids, by the reaction of glutamate synthase (gogat). although a small gene family for b ... | 2007 | 17350935 |
| osmtn encodes a 5'-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice (oryza sativa l.). | methylthioadenosine (mta) is released as a by-product of s-adenosylmethionine (adomet)-dependent reactions central to ethylene, polyamine, or phytosiderophore biosynthesis. mta is hydrolysed by methylthioadenosine nucleosidase (mtn; ec 3.2.2.16) into adenine and methylthioribose which is processed through the methionine (met) cycle to produce a new molecule of adomet. in deepwater rice, submergence enhances ethylene biosynthesis, and ethylene in turn influences the methionine cycle through posit ... | 2007 | 17339651 |
| comparative physical mapping between oryza sativa (aa genome type) and o. punctata (bb genome type). | a comparative physical map of the aa genome (oryza sativa) and the bb genome (o. punctata) was constructed by aligning a physical map of o. punctata, deduced from 63,942 bac end sequences (bess) and 34,224 fingerprints, onto the o. sativa genome sequence. the level of conservation of each chromosome between the two species was determined by calculating a ratio of bes alignments. the alignment result suggests more divergence of intergenic and repeat regions in comparison to gene-rich regions. fur ... | 2007 | 17339227 |
| the flowering locus t-like gene family in barley (hordeum vulgare). | the flowering locus t (ft) gene plays a central role in integrating flowering signals in arabidopsis because its expression is regulated antagonistically by the photoperiod and vernalization pathways. ft belongs to a family of six genes characterized by a phosphatidylethanolamine-binding protein (pebp) domain. in rice (oryza sativa), 19 pebp genes were previously described, 13 of which are ft-like genes. five ft-like genes were found in barley (hordeum vulgare). hvft1, hvft2, hvft3, and hvft4 we ... | 2007 | 17339225 |
| rice (oryza sativa) allergy in rhinitis and asthma patients: a clinico-immunological study. | sensitization to foods varies in different countries reflecting a possible interaction of genetic factors, cultural and dietary habits. rice is a major food consumed world wide and needs evaluation for ige mediated reactions. the present study was carried out to identify rice allergy in patients of rhinitis and asthma and identify the allergenic proteins in raw and cooked rice. of 1200 patients screened using standard questionnaire, 165 presented with history of rice allergy. of these, 20 (12.1% ... | 2007 | 17336834 |
| nitrite-driven anaerobic atp synthesis in barley and rice root mitochondria. | mitochondria isolated from the roots of barley (hordeum vulgare l.) and rice (oryza sativa l.) seedlings were capable of oxidizing external nadh and nadph anaerobically in the presence of nitrite. the reaction was linked to atp synthesis and nitric oxide (no) was a measurable product. the rates of nadh and nadph oxidation were in the range of 12-16 nmol min(-1) mg(-1) protein for both species. the anaerobic atp synthesis rate was 7-9 nmol min(-1) mg(-1) protein for barley and 15-17 nmol min(-1) ... | 2007 | 17333252 |
| reduced protease activity in transformed rice cell suspension cultures expressing a proteinase inhibitor. | in this study, we synthesized a synthetic serine proteinase inhibitor ii gene (spi-ii) that harbored the chymotrypsin and trypsin inhibitor domains of the pi-ii gene from nicotiana alata. in an effort to reduce protease activity in a rice cell suspension culture, we first synthesized spi-ii using overlap pcr and then introduced the gene into a rice calli (oryza sativa l. cv. dongin) by particle bombardment-mediated transformation. the spi-ii gene was under the control of a rice alpha-amylase 3d ... | 2007 | 17314052 |
| the anti-photooxidation of anthocyanins-rich leaves of a purple rice cultivar. | in the leaf of rice (oryza sativa l.) cultivar yunnan purple rice, the anthocyanins with an obvious absorption peak at 530nm were distributed in the cells of upper and lower epidermis, bulliform tissue and bristle. the maximal photosynthetic oxygen evolution rate and chlorophyll content in flag leaves were 28% and 23%, respectively, more than the common green leaf rice cultivar chijiaoruanzhan. higher chlorophyll content is probably one of the physiological adaptations for enhancing light harves ... | 2006 | 17312992 |
| heredity, physiology and mapping of a chlorophyll content gene of rice (oryza sativa l.). | a single dominant gene gc controls the trait of high chlorophyll (chl) content in rice (cultivar (cv.) zhenshan 97b). the contents of chl b and total chl increased 100% and 25%, respectively, when gc was introduced. in addition, photosynthetic rate, biomass and grain yield also increased by 20%, 17% and 16%, respectively. three simple sequence repeats (ssr) markers (rm462, rm6340 and rm6464) that are linked to gc were identified by amplification of dna samples from near-isogenic lines using two ... | 2008 | 17306416 |
| rice albumin n-terminal (asp-his-his-gln) prevents against copper ion-catalyzed oxidations. | chromatographic separation of soluble proteins from rice (oryza sativa l.) yielded a major albumin protein (16 kda), with the dhhqvyspgeq sequence in the n terminus, showing antioxidant action. the rice albumin was more potent than other rice proteins in preventing cu2+-induced low-density lipoprotein (ldl) oxidation. additionally, it also exhibited a remarkable suppression of hocl oxidation. in a further study, albumin inhibited cu2+-induced oxidation of ldl in a stoichiometric manner with an e ... | 2007 | 17305353 |
| interactions among endophytic bacteria and fungi: effects and potentials. | plants benefit extensively by harbouring endophytic microbes. they promote plant growth and confer enhanced resistance to various pathogens. however, the way the interactions among endophytes influence the plant productivity has not been explained. present study experimentally showed that endophytes isolated from rice (oryza sativa) used as the test plant produced two types of interactions; biofilms (bacteria attached to mycelia) and mixed cultures with no such attachments. acidity, as measured ... | 2006 | 17301503 |
| identification and mapping of expressed genes, simple sequence repeats and transposable elements in centromeric regions of rice chromosomes. | the genomic sequences derived from rice centromeric regions were analyzed to facilitate the comprehensive understanding of the rice genome. a rice centromere-specific satellite sequence, rcs2/trsd/cento, was used to screen p1-derived artificial chromosome (pac) and bacterial artificial chromosome (bac) genomic libraries derived from oryza sativa l. ssp. japonica cultivar nipponbare. physical maps of the centromeric regions were constructed by dna fingerprinting methods and the aligned clones wer ... | 2006 | 17298954 |
| knockout of a starch synthase gene osssiiia/flo5 causes white-core floury endosperm in rice (oryza sativa l.). | to elucidate the role of ssiiia during starch synthesis in rice (oryza sativa l.) endosperm, we characterized null mutants of this gene, generated by t-dna insertions. scanning electron microscope (sem) analysis revealed that the starch granules in these mutants are smaller and rounder compared with the wild type controls, and that the mutant endosperm is characterized by a loosely packed central portion exhibiting a floury-like phenotype. hence, the osssiiia (oryza sativa ssiiia) mutations are ... | 2007 | 17297616 |
| computational identification and phylogenetic analysis of the mapk gene family in oryza sativa. | the mapk cascade plays greatly important roles in signal transduction pathways. the present study computationally identified 16 rice mapks (osmpks). the results of est and cdna hitting supported the reliability of osmpks in rice. gene structure comparison showed large differences in exon numbers, from 2 to 12, among members of the rice mapk family. rice mapks were located on chromosomes 1, 2, 3, 5, 6, 10, and 11, particularly being distributed on the 1, 5 and 6 chromosomes. on a genome scale we ... | 2007 | 17296305 |
| oryzacystatin-ii, a cystatin from rice (oryza sativa l. japonica), is a dimeric protein: possible involvement of the interconversion between dimer and monomer in the regulation of the reactivity of oryzacystatin-ii. | we examined the biochemical and structural properties of oryzacystatin-ii, a phytocystatin in rice (oryza sativa l. japonica), under heat-stress conditions. the enzyme inhibitory reactivity of oryzacystatin-ii was enhanced by heating in a temperature-dependent manner and reached a maximum level by heating at 65 degrees c for 10 min. size-exclusion chromatography showed that oryzacystatin-ii forms a homodimer at ambient temperature and that the enhancement of inhibitory reactivity is due to the c ... | 2007 | 17295505 |
| rice as a model for centromere and heterochromatin research. | rice (oryza sativa) has become an important model plant species in numerous research projects involving genome, molecular and evolutionary biology. in this review we describe the reasons why rice provides an excellent model system for centromere and heterochromatin research. in most multicellular eukaryotes, centromeres and heterochromatic domains contain long arrays of repetitive dna elements that are recalcitrant to dna sequencing. in contrast, three rice centromeres and the majority of the cy ... | 2007 | 17295128 |
| f-box proteins in rice. genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress. | f-box proteins constitute a large family in eukaryotes and are characterized by a conserved f-box motif (approximately 40 amino acids). as components of the skp1p-cullin-f-box complex, f-box proteins are critical for the controlled degradation of cellular proteins. we have identified 687 potential f-box proteins in rice (oryza sativa), the model monocotyledonous plant, by a reiterative database search. computational analysis revealed the presence of several other functional domains, including le ... | 2007 | 17293439 |
| overexpression of a type-a response regulator alters rice morphology and cytokinin metabolism. | genome-wide analyses of rice (oryza sativa l.) cytokinin (ck)-responsive genes using the affymetrix genechip(r) rice genome array were conducted to define the spectrum of genes subject to regulation by ck in monocotyledonous plants. application of trans-zeatin modulated the expression of a wide variety of genes including those involved in hormone signaling and metabolism, transcriptional regulation, macronutrient transport and protein synthesis. to understand further the function of ck in rice p ... | 2007 | 17293362 |
| oryza sativa myosin xi b controls pollen development by photoperiod-sensitive protein localizations. | myosins are actin-based motor proteins responsible for various motility and signal transduction. only a small set of myosin classes is present inplants, and little is known about their functions. here we showed how a rice myosin gene controlled pollen development by sensing changed environmental factors. the analysis is based on a gene-trapped ds insertion mutant oryza sativa myosin xi b (osmyoxib). this mutant showed male sterility under short day length (sd) conditions and fertility under long ... | 2007 | 17289016 |
| two novel loci for pollen sterility in hybrids between the weedy strain ludao and the japonica variety akihikari of rice (oryza sativa l.). | partial pollen sterility has been observed in hybrid progeny derived from a japonica cultivar, akihikari and a weedy strain, ludao, which naturally grows in jiangsu province of east china. cytological and histological analyses revealed that pollen abortion occurred largely at the bicellular pollen stage, primarily due to the gradual disaggregation of generative and vegetative cells. a genome-wide analysis was further carried out in a backcross population of akihikari //ludao/akihikari using a to ... | 2007 | 17287976 |
| fine mapping of pss1, a pollen semi-sterile gene in rice (oryza sativa l.). | during routine seed increase procedures in rice, semi-sterile plants are common; however, such semi-sterility mutants in rice varieties have been only rarely analyzed genetically. w207-2 is a semi-sterile selection from the japonica rice variety nipponbare. in this report, we found the female gamete of w207-2 was normal, and its semi-sterility was unaffected by growth duration but was conditioned by a recessive nuclear gene whose action leads to pollen semi-sterility and anther indehiscence, and ... | 2007 | 17279367 |
| in silico modeling and hydrogen peroxide binding study of rice catalase. | homology modeling of the catalase, catc cloned and sequenced from rice (oryza sativa l., cv ratna an indica cultivar) has been performed based on the crystal structure of the catalase catf (pdb code 1m7s) by using the software modeller. with the aid of molecular mechanics and molecular dynamics methods, the final model is obtained and is further assessed by procheck and verify - 3d graph, which show that the final refined model is reliable. with this model, a flexible docking study with the hydr ... | 2006 | 17274773 |
| the discriminatory transfer routes of trna genes among organellar and nuclear genomes in flowering plants: a genome-wide investigation of indica rice. | the transfer and integration of trna genes from organellar genomes to the nuclear genome and between organellar genomes occur extensively in flowering plants. the routes of the genetic materials flowing from one genome to another are biased, limited largely by compatibility of dna replication and repair systems differing among the organelles and nucleus. after thoroughly surveying the trna gene transfer among organellar genomes and the nuclear genome of a domesticated rice (oryza sativa l. ssp. ... | 2007 | 17273918 |
| [effect of free-air co2 enrichment (face) on leaf nitrate reductase activity of oryza sativa l. cultivar wuxianjing 14]. | with the target co2 concentration of face plots being 200 micromol x mol(-1) above that in ambient air, this paper studied the effect of free-air co2 enrichment (face) on leaf nitrate reductase activity (nra) of oryza sativa l. cultivar wuxianjing 14. the results showed that face obviously increased the nra of functional leaves at all growth stages, with an average increment of 50%, 20%, 60%, 80% and 30% at the stages of jointing, booting, heading, 10 after heading, and 20 days after heading, re ... | 2006 | 17269350 |
| genome-wide identification and analyses of the rice calmodulin and related potential calcium sensor proteins. | a wide range of stimuli evoke rapid and transient increases in [ca2+]cyt in plant cells which are transmitted by protein sensors that contain ef-hand motifs. here, a group of oryza sativa l. genes encoding calmodulin (cam) and cam-like (cml) proteins that do not possess functional domains other than the ca2+-binding ef-hand motifs was analyzed. | 2007 | 17263873 |
| distinct signalling pathways for induction of map kinase activities by cadmium and copper in rice roots. | plant growth is severely affected by toxic concentrations of heavy metals. on characterizing the heavy metal-induced signalling pathways, the effects of cadmium (cdcl2) and copper (cucl2) on mbp (myelin basic protein) kinase activities in oryza sativa l. cv. tng67 were analysed and it was found that cd2+-induced 42 kda mbp kinase has the characteristics of a mitogen-activated protein (map) kinase. this study confirmed that the 42 kda kinase-active band contains, at least, the activities of osmpk ... | 2007 | 17259646 |
| spatial distribution and temporal variation of the rice silicon transporter lsi1. | rice (oryza sativa) is a typical silicon (si) accumulator and requires a large amount of si for high-yield production. recently, a gene (low silicon rice1 [lsi1]) encoding a si transporter was identified in rice roots. here, we characterized lsi1 in terms of spatial distribution and temporal variation using both physiological and molecular approaches. results from a multicompartment transport box experiment showed that the major site for si uptake was located at the basal zone (>10 mm from the r ... | 2007 | 17259286 |
| cultivar identification of rice (oryza sativa l.) by polymerase chain reaction method and its application to processed rice products. | as the cultivars of rice markedly affect eating quality, processing suitability, and price, identification or differentiation of rice cultivar is very important. we developed suitable 14 sts (sequence-tagged site) primers for pcr (polymerase chain reaction), and it became possible to differentiate 60 japanese dominant rice cultivars from each other using template dna extracted and purified from rice grains. a multiplex primer set was shown to be useful to effectively differentiate rice cultivars ... | 2007 | 17256960 |
| rapid discrimination of scented rice by solid-phase microextraction, mass spectrometry, and multivariate analysis used as a mass sensor. | this study describes a new and suitable method for the rapid evaluation of rice (oryza sativa, l.) aroma by analysis of the volatile fraction using solid-phase microextraction coupled with mass spectrometry (spme/ms). the abundance list of unresolved mass fragments of the spme extracted volatile fraction formed the "fingerprint" of a rice sample. fingerprints of 61 rice samples were recorded in duplicate. pollutants originating from the extraction system induce fingerprint background that could ... | 2007 | 17256955 |
| identification of drought-induced micrornas in rice. | micrornas (mirnas) are a large new class of small non-coding rnas. to date, hundreds of micrornas have been identified in plants. micrornas play important roles in post-transcriptional gene regulation by targeting mrnas for cleavage or repressing translation. to better understand microrna function, we have used an oligonucleotide microarray to monitor rice (oryza sativa) microrna expression profile under drought stress. two drought-induced micrornas were identified. furthermore, mir-169g was con ... | 2007 | 17254555 |
| chemically induced expression of rice osb2 under the control of the ospr1.1 promoter confers increased anthocyanin accumulation in transgenic rice. | anthocyanin pigmentation provides an excellent system with which to study the regulation of gene expression in higher plants. in this study, ospr1.1 promoter was isolated and the promoter activity was monitored using a reporter gene osb2, which encodes a transcription factor for anthocyanin synthesis in rice plants. we introduced pr::osb2 plasmid into an isogenic taichung 65, no. 99-962 t-65 cba b9f5 (t65 cba), rice line (oryza sativa l.) and found that the transgenic rice plants exhibited antho ... | 2007 | 17253710 |
| expression of an nadp-malic enzyme gene in rice (oryza sativa. l) is induced by environmental stresses; over-expression of the gene in arabidopsis confers salt and osmotic stress tolerance. | nadp-malic enzyme (nadp-me, ec 1.1.1.40) functions in many different pathways in plants, and has recently been implicated in plant defense such as in responses to wounding and uv-b radiation. in this study, we isolated a complementary dna (cdna) clone by using the differential display method and screening of a root cdna library of rice (oryza sativa. l) under carbonate (nahco3) stress, and identified it as one of the rice nadp-me genes (we named it nadp-me2, genbank accession no. ab053295). the ... | 2007 | 17245561 |
| transport mechanisms for the uptake of organic compounds by rice (oryza sativa) roots. | uptake of six organic compounds, dinitrobenzene (dnb), dinitrotoluene (dnt), lindan (lin), 1,2,3-triclorobenzene (tcb), phenanthrene (phn) and pyrene (pyr) by freshly excised rice roots and dead rice roots (heated for 40min at 105 degrees c) were investigated. results indicated that the uptake by the two types of roots did not increase proportionally with those in external solution. there appears to be some special chemical function of root cells other than simple absorption by the cells. the co ... | 2007 | 17240019 |
| biased distribution of microsatellite motifs in the rice genome. | microsatellites are useful tools to study the extent of divergence between two taxonomic groups that show high sequence similarity. we have compared microsatellite distribution to illustrate genetic variation between the two rice genomes, oryza sativa l. ssp. indica and oryza sativa l. ssp. japonica. microsatellite distribution proved to be non random as certain regions of very high microsatellite density have been identified. microsatellite density in the subspecies japonica was computed margin ... | 2007 | 17237941 |
| genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice. | mutant genes, reduced culm number 1 (rcn1) and bunketsuwaito tillering dwarf (d3), affect tiller number in rice (oryza sativa l.) in opposite directions. the d3 mutant was reported to increase tiller number and reduce plant stature. our objective was to compare the phenotype of the d3rcn1 double mutant with each single mutant and parental rice cultivar "shiokari" and to clarify whether the rcn1 gene interacted with the d3 gene. we recovered a new rcn1 mutant from shiokari and developed d3rcn1 do ... | 2015 | 17237471 |
| a honglian cms line of rice displays aberrant f0 of f0f1-atpase. | honglian (hl) is one of the three major types of cytoplasmic male sterility (cms) of rice (oryza sativa l) that has been commercially used in hybrid production. in previous studies, the cms in hl is shown to be associated with a chimeric gene orfh79 that is cotranscribed with an extra atp6 in mitochondria. this study demonstrated that the intact f0f1-atpase in hl cms line was specifically reduced in both of its protein quantity and enzyme activity, whereas its f1 sector was not affected. it impl ... | 2007 | 17226056 |
| characterization of a stress responsive proteinase inhibitor gene with positive effect in improving drought resistance in rice. | a full-length cdna gene, designated oryza sativa chymotrypsin inhibitor-like 1 (ocpi1), was characterized in rice. the predicted protein of ocpi1 shows very high sequence identity to reported chymotrypsin inhibitors from various plant species. northern-blot analysis showed that the expression of ocpi1 was strongly induced by dehydration stresses and abscisic acid (aba). the expression of beta-glucuronidase (gus) reporter gene under the control of ocpi1 promoter transformed into rice was strongly ... | 2007 | 17221232 |
| recent proliferation and translocation of pollen group 1 allergen genes in the maize genome. | the dominant allergenic components of grass pollen are known by immunologists as group 1 allergens. these constitute a set of closely related proteins from the beta-expansin family and have been shown to have cell wall-loosening activity. group 1 allergens may facilitate the penetration of pollen tubes through the grass stigma and style. in maize (zea mays), group 1 allergens are divided into two classes, a and b. we have identified 15 genes encoding group 1 allergens in maize, 11 genes in class ... | 2007 | 17220362 |
| large-scale dna polymorphism study of oryza sativa and o. rufipogon reveals the origin and divergence of asian rice. | polymorphism over approximately 26 kb of dna sequence spanning 22 loci and one region distributed on chromosomes 1, 2, 3 and 4 was studied in 30 accessions of cultivated rice, oryza sativa, and its wild relatives. phylogenetic analysis using all the dna sequences suggested that o. sativa ssp. indica and ssp. japonica were independently domesticated from a wild species o. rufipogon. o. sativa ssp. indica contained substantial genetic diversity (pi = 0.0024), whereas ssp. japonica exhibited extrem ... | 2007 | 17219210 |
| generation and characterization of low phytic acid germplasm in rice (oryza sativa l.). | phytic acid (pa, myo-inositol 1,2,3,4,5,6-hexakisphosphate), or its salt form, phytate, is commonly regarded as the major anti-nutritional component in cereal and legume grains. breeding of low phytic acid (lpa) crops has recently been considered as a potential way to increase nutritional quality of crop products. in this study, eight independent lpa rice mutant lines from both indica and japonica subspecies were developed through physical and chemical mutagenesis. among them, five are non-letha ... | 2007 | 17219209 |
| isolation of cold stress-responsive genes in the reproductive organs, and characterization of the oslti6b gene from rice (oryza sativa l.). | during their reproductive stage, rice crops often are exposed to cold stress, which leads to sterility and reduced yields. to understand the cold response mechanism at that stage, we used an mrna differential display method to isolate cold-responsive genes from pre-anthesis flowers. approximately 5,000 transcripts were identified here, of which 123 were found to be displayed differentially between the control (30 degrees c) and cold-treated (12 degrees c) flowers. among them, 26 were analyzed by ... | 2007 | 17219102 |
| structural and functional analyses of the wheat genomes based on expressed sequence tags (ests) related to abiotic stresses. | to gain insights into the structure and function of the wheat (triticum aestivum l.) genomes, we identified 278 ests related to abiotic stress (cold, heat, drought, salinity, and aluminum) from 7671 ests previously mapped to wheat chromosomes. of the 278 abiotic stress related ests, 259 (811 loci) were assigned to chromosome deletion bins and analyzed for their distribution pattern among the 7 homoeologous chromosome groups. distribution of abiotic stress related est loci were not uniform throug ... | 2006 | 17218960 |
| cloning and characterization of two cytochrome p450 cyp6ax1 and cyp6ay1 cdnas from nilaparvata lugens stål (homoptera: delphacidae). | two full-length p450 cdnas, cyp6ax1 and cyp6ay1, were cloned from the brown planthopper nilaparvata lugens stål (homoptera: delphacidae). both cyp6ax1 and cyp6ay1 are typical microsomal p450s and their deduced amino acid sequences share common characteristics with other members of the insect p450 cyp6 family. cyp6ax1 and cyp6ay1 show the highest percent identity (36%) of amino acid to each other; both of them have 31-33% amino acid identity with cyp6b1 from papilio polyxenes (lepidoptera: papili ... | 2007 | 17212353 |
| submergence tolerance in rice requires sub1a, an ethylene-response-factor-like gene. | submergence of rice (oryza sativa) by flash flooding is a major constraint to rice production in asia. rice cultivars vary in their capacity to tolerate complete submergence; quantitative trait loci analyses have revealed that a large portion of this variation in submergence tolerance can be explained by one locus (sub1) on chromosome 9. two recently published papers (takeshi fukao et al. and kenong xu et al.) present evidence that a transcription factor belonging to the b-2 subgroup of the ethy ... | 2007 | 17208508 |
| two native pools of phytochrome a in monocots: evidence from fluorescence investigations of phytochrome mutants of rice. | fluorescence investigations of phytochrome (phy) in rice (oryza sativa l. cv. nipponbare) mutants deficient in phya, phyb and phya plus phyb were performed. total content of the pigment (p(tot)) and its spectroscopic and photochemical characteristics were determined in different parts of the dark-grown and far-red light (fr)-grown coleoptiles. spectroscopically, phya in the phyb mutant was identical to phya in the wild-type (wt) and the extent of the conversion from pr to lumi-r at 85 k was the ... | 2015 | 17205634 |
| function and diversification of mads-box genes in rice. | mads-box genes play critical roles in a number of developmental processes in flowering plants, such as specification of floral organ identity, control of flowering time, and regulation of fruit development. because of their crucial functions in flower development, diversification of the mads-box gene family has been suggested to be a major factor responsible for floral diversity during radiation of the flowering plants. inflorescences and flowers in the grass species have unique structures that ... | 2006 | 17205197 |
| a model explaining genotypic and ontogenetic variation of leaf photosynthetic rate in rice (oryza sativa) based on leaf nitrogen content and stomatal conductance. | identification of physiological traits associated with leaf photosynthetic rate (pn) is important for improving potential productivity of rice (oryza sativa). the objectives of this study were to develop a model which can explain genotypic variation and ontogenetic change of pn in rice under optimal conditions as a function of leaf nitrogen content per unit area (n) and stomatal conductance (g(s)), and to quantify the effects of interaction between n and g(s) on the variation of pn. | 2007 | 17204541 |
| dramatic reduction of crop-to-crop gene flow within a short distance from transgenic rice fields. | genetically modified (gm) rice with enhanced agronomic traits and pharmaceutical uses are ready for widespread adoption. little is known about isolation requirements for achieving stringent transgene confinement in rice. to investigate the extent of pollen-mediated crop-to-crop transgene flow, we conducted a field experiment with four plot-size treatments of adjacent gm and nongm rice (oryza sativa) in china. three insect-resistant gm rice (bt/cpti) and nongm isogenic lines were used in the stud ... | 2007 | 17204081 |
| nanostructural analysis of starch components by atomic force microscopy. | morphological and structural features of starch from potato (solanum tuberosa) and rice (oryza sativa) have been examined using atomic force microscopy. amylose from potato and rice was observed in aggregated structures, which are suggested to be a result of retrogradation during sample preparation. the degrees of polymerization of amylose from potato and rice starches were calculated from the mean contour lengths of the observed structures to be approximately 1440 and 1860, respectively. potato ... | 2006 | 17204065 |
| proteome dynamics during plastid differentiation in rice. | we have analyzed proteome dynamics during light-induced development of rice (oryza sativa) chloroplasts from etioplasts using quantitative two-dimensional gel electrophoresis and tandem mass spectrometry protein identification. in the dark, the etioplast allocates the main proportion of total protein mass to carbohydrate and amino acid metabolism and a surprisingly high number of proteins to the regulation and expression of plastid genes. chaperones, proteins for photosynthetic energy metabolism ... | 2007 | 17189339 |
| unique properties of nadp-thioredoxin reductase c in legumes. | nadp-thioredoxin reductases (ntrs) reduce thioredoxins (trxs), using nadph as a reductant, together constituting complete redox systems (nts). beside ntra and ntrb targeted to both cytosol and mitochondria of plant cells, there is in chloroplasts an unusual ntr (ntrc) harbouring a trx domain in a c-terminal extension, as recently reported in oryza sativa. although ntrc may constitute a complete nts, it was described as a bifunctional enzyme. because the gene is only present in photosynthetic org ... | 2007 | 17185738 |
| analysis of the phylogenetic relationships among several species of gramineae using acgm markers. | to study the transferability of rice (oryza sativa l.) genome data, we used amplified consensus genetic markers to analyze the phylogenetic relationships among several species and genera in gramineae. ten accessions representing five grass genera (oryza, zea, setaria, triticum, and phyllostachys) were used. according to the genetic distances, a cluster tree was constructed. the relationships among the five genera could be simply described as ((oryza + (zea +setaria)) +triticum) +phyllostachys. t ... | 2006 | 17185173 |
| ultrastructure and gene mapping of the albino mutant al12 in rice (oryza sativa l.). | seedling albino mutation resistant to low temperature is an adaptability of rice (oryza sativa l.) to cold. the mutant, a conditional expression controlled by development and temperature, differs from other albino mutants. the chlorophyll content of the mutant was measured using a portable chlorophyll meter, and the ultrastructure of the chloroplast was observed using a transmission electron microscope. chlorophyll content was 1.2 spad, and the chloroplast did not develop, with only small vesicl ... | 2006 | 17185171 |
| impact assessment of cadmium contamination on rice (oryza sativa l.) seedlings at molecular and population levels using multiple biomarkers. | assessment of environmental contamination on ecology (plant) at molecular and population levels is important in risk quantification and remediation study. random amplified polymorphic dna (rapd) assay and related other fingerprinting techniques have been employed to detect the genotoxin-induced dna damage and mutations. this research compared the effects occurring at molecular and population levels in rice seedlings exposed to cadmium (cd) concentrations of 15-60 mg l(-1) for 8 days with quartz ... | 2007 | 17184821 |
| analysis of gene function in rice through virus-induced gene silencing. | virus-induced gene silencing (vigs) is a powerful rna-silencing based technology adapted for the study of host-gene function. vigs functions through the expression of a host gene from a virus vector. both the virus-encoded host sequence and the homologous host target messenger rna are destroyed or made inactive through a host surveillance system. here, we describe procedures for the use of a new virus vector for vigs in monocotyledonous hosts and, in particular, in rice (oryza sativa), a species ... | 2007 | 17172752 |
| the rice kinase database. a phylogenomic database for the rice kinome. | the rice (oryza sativa) genome contains 1,429 protein kinases, the vast majority of which have unknown functions. we created a phylogenomic database (http://rkd.ucdavis.edu) to facilitate functional analysis of this large gene family. sequence and genomic data, including gene expression data and protein-protein interaction maps, can be displayed for each selected kinase in the context of a phylogenetic tree allowing for comparative analysis both within and between large kinase subfamilies. inter ... | 2007 | 17172291 |
| a bidirectional gene trap construct suitable for t-dna and ds-mediated insertional mutagenesis in rice (oryza sativa l.). | a construct suitable for genome-wide transfer-dna (t-dna) and subsequent transposon-based (ds) gene trapping has been developed for use in rice (oryza sativa). this t-dna/ds construct contains: ds terminal sequences immediately inside t-dna borders for subsequent ds mobilization; promoterless green fluorescent protein (sgfps65t) and beta-glucuronidase (uida) reporter genes, each fused to an intron (from arabidopsis gpa1 gene) to enable bidirectional gene trapping by t-dna or ds; an ampicillin re ... | 2004 | 17168884 |
| growth performance and biochemical responses of three rice (oryza sativa l.) cultivars grown in fly-ash amended soil. | the disposal of fly-ash (fa) from coal-fired power stations causes significant economic and environmental problems. use of such contaminated sites for crop production and use of contaminated water for irrigation not only decreases crop productivity but also poses health hazards to humans due to accumulation of toxic metals in edible grains. in the present investigation, three rice cultivars viz., saryu-52, sabha-5204, and pant-4 were grown in garden soil (gs, control) and various amendments (10% ... | 2007 | 17166555 |
| global transcript analysis of rice leaf and seed using sage technology. | we have compiled two comprehensive gene expression profiles from mature leaf and immature seed tissue of rice (oryza sativa ssp. japonica cultivar nipponbare) using serial analysis of gene expression (sage) technology. analysis revealed a total of 50 519 sage tags, corresponding to 15 131 unique transcripts. of these, the large majority (approximately 70%) occur only once in both libraries. unexpectedly, the most abundant transcript (approximately 3% of the total) in the leaf library was derived ... | 2003 | 17163904 |
| proteomic responses of rice young panicles to salinity. | rice (oryza sativa) is most sensitive to salinity during the reproductive stage. we employed a proteomic approach to further understand the mechanism of plant responses to salinity at an early reproductive stage. plants were grown in culture solution and salt stress imposed at panicle initiation. after 12 days of stress, young panicles were collected from control and salt stressed plants. the na+ and k+ content of panicle and several yield components changed significantly in response to short-te ... | 2006 | 17163441 |
| two novel nuclear genes, ossig5 and ossig6, encoding potential plastid sigma factors of rna polymerase in rice: tissue-specific and light-responsive gene expression. | two novel nuclear genes, ossig5 and ossig6, encoding potential plastid sigma factors of rna polymerase (rnap) were identified in oryza sativa. the deduced amino acid sequences contain conserved regions, regions 1.2-4.2, and a novel region a/b at the n-terminus. tissue-specific and light-responsive transcripts of ossig5 and ossig6 were observed. the n-terminal region of ossig5 conferred import of green fluorescent protein into the chloroplast. specific transcripts of rice psba were synthesized in ... | 2007 | 17148693 |
| transgenic rice lines expressing maize c1 and r-s regulatory genes produce various flavonoids in the endosperm. | flavonoids, compounds that possess diverse health-promoting benefits, are lacking in the endosperm of rice. therefore, to develop transgenic lines that produce flavonoids, we transformed a white rice cultivar, oryza sativa japonica cv. hwa-young, with maize c1 and r-s regulatory genes. expression of these transgenes was restricted to the endosperm using the promoter of a rice prolamin gene. the pericarp of the c1/r-s homozygous lines became dark brown in accordance with their maternal genotype, ... | 2006 | 17147636 |
| a paracentric inversion suppresses genetic recombination at the fon3 locus with breakpoints corresponding to sequence gaps on rice chromosome 11l. | paracentric inversion is known to inhibit genetic recombination between normal and inverted chromosomal segments in heterozygous arrangements. insect inversion polymorphisms have been studied to reveal adaptive processes for maintaining genetic variation. we report the first paracentric inversion in rice (oryza sativa), which was discovered in our effort to clone the floral organ number gene fon3. recombination at the fon3 locus on the long arm of chromosome 11 was severely suppressed over a dis ... | 2007 | 17143630 |
| functional characterization of the rice kaurene synthase-like gene family. | the rice (oryza sativa) genome contains a family of kaurene synthase-like genes (osksl) presumably involved in diterpenoid biosynthesis. while a number of osksl enzymes have been functionally characterized, several have not been previously investigated, and the gene family has not been broadly analyzed. here we report cloning of several osksl genes and functional characterization of the encoded enzymes. in particular, we have verified the expected production of ent-kaur-16-ene by the gibberellin ... | 2007 | 17141283 |
| isolation of stress responsive psb a gene from rice (oryza sativa l.) using differential display. | differential display (dd) experiments were performed on drought-tolerant rice (oryza sativa l.) genotype n22 to identify both upregulated and downregulated partial cdnas with respect to moisture stress. dna polymorphism was detected between drought-stressed and control leaf tissues on the dd gels. a partial cdna showing differential expression, with respect to moisture stress was isolated from the gel. northern blotting analysis was performed using this cdna as a probe and it was observed that m ... | 2006 | 17133769 |
| identification of stress-responsive genes in an indica rice (oryza sativa l.) using ests generated from drought-stressed seedlings. | the impacts of drought on plant growth and development limit cereal crop production worldwide. rice (oryza sativa) productivity and production is severely affected due to recurrent droughts in almost all agroecological zones. with the advent of molecular and genomic technologies, emphasis is now placed on understanding the mechanisms of genetic control of the drought-stress response. in order to identify genes associated with water-stress response in rice, ests generated from a normalized cdna l ... | 2007 | 17132712 |
| proteomics identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated oryza sativa pollen. | mature pollen from most plant species is metabolically quiescent; however, after pollination, it germinates quickly and gives rise to a pollen tube to transport sperms into the embryo sac. because methods for collecting a large amount of in vitro germinated pollen grains for transcriptomics and proteomics studies from model plants of arabidopsis and rice are not available, molecular information about the germination developmental process is lacking. here we describe a method for obtaining a larg ... | 2007 | 17132620 |
| differentiation of the two rice subspecies indica and japonica: a gene ontology perspective. | cultivated rice (oryza sativa) is comprised of two subspecies: japonica and indica. polymorphism levels between putative homologues were determined for genes whose japonica homologue had been classified into functional categories using the gene ontology (go) system. genes were partitioned into below-average and above-average polymorphism groups, and then the set of genes having each go term was checked for the randomness of its distribution into these polymorphism groups using a series of false ... | 2007 | 17131138 |
| qtl mapping of grain length in rice (oryza sativa l.) using chromosome segment substitution lines. | chromosome segment substitution (css) lines have the potential for use in qtl fine mapping and map-based cloning. the standard t-test used in the idealized case that each css line has a single segment from the donor parent is not suitable for non-idealized css lines carrying several substituted segments from the donor parent. in this study, we present a likelihood ratio test based on stepwise regression (rstep-lrt) that can be used for qtl mapping in a population consisting of non-idealized css ... | 2006 | 17125584 |
| photon emissions from rice cells elicited by n-acetylchitooligosaccharide are generated through phospholipid signaling in close association with the production of reactive oxygen species. | biophotons are ultraweak light emissions from biochemical reactions in a living body. they increase in suspension-cultured rice (oryza sativa l.) cells when elicited by n-acetylchitooligosaccharide. biochemical analyses were undertaken to investigate the relationship between disease response and biophotons in order to clarify the emission mechanism of biophotons caused by this elicitor. photon emissions induced by n-acetylchitohexaose were suppressed when cells were pretreated with the reactive ... | 2016 | 17123826 |
| transcriptomic adaptations in rice suspension cells under sucrose starvation. | sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion and senescence; however, that of a monocot model plant, rice, under sucrose depletion is still under investigation. here, the time-course monitoring of gene expression profiles in ... | 2007 | 17115300 |
| analysis of genotype x environment interaction effects for starch pasting viscosity characteristics in indica rice. | milled rice (oryza sativa l.) is composed of approximately 90% starch. the properties of starch have considerable effects on cooked rice palatability and consumer acceptability. starch pasting viscosity parameters serve as important indices in the estimation of eating, cooking, and processing qualities of rice. in the present study, four cytoplasmic male-sterile (cms) lines and eight restorer (r) lines have been used in an incomplete diallel cross to analyze seed effects, cytoplasmic effects, ma ... | 2006 | 17112972 |
| genomic variation in rice: genesis of highly polymorphic linkage blocks during domestication. | genomic regions that are unusually divergent between closely related species or racial groups can be particularly informative about the process of speciation or the operation of natural selection. the two sequenced genomes of cultivated asian rice, oryza sativa, reveal that at least 6% of the genomes are unusually divergent. sequencing of ten unlinked loci from the highly divergent regions consistently identified two highly divergent haplotypes with each locus in nearly complete linkage disequil ... | 2006 | 17112320 |
| compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice (oryza sativa l). | the shoot apical meristem (sam) produces lateral organs in a regular spacing (phyllotaxy) and at a regular interval (phyllochron) during the vegetative phase. in a dissociation (ds) insertion rice population, we identified a mutant, compact shoot and leafy head 1 (csl1), which produced massive number of leaves (~70) during the vegetative phase. in csl1, the transition from the vegetative to the reproductive phase was delayed by about 2 months under long-day conditions. with a reduced leaf size a ... | 2007 | 17111113 |
| pattern and rate of indel evolution inferred from whole chloroplast intergenic regions in sugarcane, maize and rice. | microstructural changes such as insertions and deletions (=indels) are a major driving force in the evolution of non-coding dna sequences. to better understand the mechanisms by which indel mutations arise, as well as the molecular evolution of non-coding regions, the number and pattern of indels and nucleotide substitutions were compared in the whole chloroplast genomes. comparisons were made for a total of over 38 kb non-coding dna sequences from 126 intergenic regions in two data sets represe ... | 2006 | 17110395 |
| low cost sorbents for the removal of methyl parathion pesticide from aqueous solutions. | sorptive potential of selected agricultural waste materials i.e. rice (oryza sativa) bran (rb), bagasse fly ash (bfa) of sugarcane (saccharum officinarum), moringa oleifera pods (mop) and rice husk (rh) for the removal of methyl parathion pesticide (mp) from surface and ground waters has been investigated. optimization of operating parameters of sorption process, i.e. sorbent dose, agitation time, ph, initial concentration of sorbate, and temperature have been studied. the sorption data fitted t ... | 2007 | 17109916 |
| co-bombardment, integration and expression of rice chitinase and thaumatin-like protein genes in barley (hordeum vulgare cv. conlon). | pathogenesis-related (pr) proteins associated with degradation of structural components of pathogenic filamentous fungi were overexpressed in the two-rowed malting barley (hordeum vulgare l.) cultivar conlon. transgenes were introduced by co-bombardment with two plasmids, one carrying a rice (oryza sativa l.) chitinase gene (chi11) and another carrying a rice thaumatin-like protein gene (tlp). each gene was under the control of the maize ubiquitin (ubi1) promoter. fifty-eight primary transforman ... | 2007 | 17103003 |
| differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. | responses of antioxidative defense systems to chilling and drought stresses were comparatively studied in four cultivars of rice (oryza sativa l.) differing in sensitivity, two of them (xiangnuo no. 1 and zimanuo) are tolerant to chilling but sensitive to drought and the other two (xiangzhongxian no. 2 and ir50) are tolerant to drought but sensitive to chilling. the seedlings of rice were transferred into growth chamber for 5 d at 8 degrees c as chilling treatment, or at 28 degrees c as control, ... | 2016 | 17098438 |
| expressions of oshkt1, oshkt2, and osvha are differentially regulated under nacl stress in salt-sensitive and salt-tolerant rice (oryza sativa l.) cultivars. | under nacl-dominated salt stress, the key to plant survival is maintaining a low cytosolic na(+) level or na(+)/k(+) ratio. the oshkt1, oshkt2, and osvha transporter genes might play important roles in maintaining cytosolic na(+) homeostasis in rice (oryza sativa l. indica cvs pokkali and brri dhan29). upon nacl stress, the oshkt1 transcript was significantly down-regulated in salt-tolerant cv. pokkali, but not in salt-sensitive cv. brri dhan29. nacl stress induced the expression of oshkt2 and o ... | 2006 | 17088362 |
| circumnutation of rice coleoptiles: its relationships with gravitropism and absence in lazy mutants. | although circumnutation occurs widely in higher plants, its mechanism is little understood. the idea that circumnutation is based on gravitropism has long been investigated, but the reported results have been controversial. we used dark-grown coleoptiles of rice (oryza sativa l.) to re-investigate this issue. the following results supported the existence of a close relationship between gravitropism and circumnutation: (1) circumnutation disappears on a horizontal clinostat; (2) circumnutation is ... | 2006 | 17087462 |
| photosynthetic and biochemical activities in flag leaves of a newly developed superhigh-yield hybrid rice (oryza sativa) and its parents during the reproductive stage. | responses of net photosynthetic rates to intercellular co(2) concentration (p (n)/c (i) curves) and photochemical characteristics were investigated in flag leaves of newly developed superhigh-yield hybrid rice (oryza sativa l.) liangyoupeijiu (lypj) and its maternal peiai64s (pa64s) and paternal wumang9311 (wm9311) lines grown in the field during the reproductive stage. the results showed that photosynthetic functions, such as the electron transport activities of photosystems and photophosphoryl ... | 2007 | 17077941 |
| [identification of the rice (oryza sativa l.) mutant msp1-4 and expression analysis of its udt1 and gamyb genes]. | a rice male-sterile mutant msp1-4 (multiple sporocyte) with japonica cultivar '9522' background, was obtained in m(3) population treated with (60)co gamma-ray. results of genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive locus. to map this locus, an f(2) population was constructed from the cross between the msp1-4 (japonica) and 'longtefu b' (indica). this locus was mapped between the two indel markers, wy-4 and wy-8, with physical distance of 247 kb ... | 2006 | 17075175 |
| genetic analysis of rooting ability of transplanted rice (oryza sativa l.) under different water conditions. | in order to assess the benefits of superior rooting ability of rice (oryza sativa l.) for growth after transplanting under water-limiting conditions, genetic differences in the rooting ability of rice seedlings 30 d after sowing, with their visible roots either pruned or not pruned, were quantified by several root parameters 4 d after transplanting (dat), under flooded or non-flooded paddy fields (four treatments in total), together with production traits at maturity. ninety-eight recombinant in ... | 2007 | 17075079 |
| distinct reorganization of the genome transcription associates with organogenesis of somatic embryo, shoots, and roots in rice. | most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. however, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. here we used a rice (oryza sativa) cell culture system to generate somatic embryos, which were further induced into shoots and roots. the global transcriptional reorganization during the development of somatic embryos, shoots, and ro ... | 2007 | 17072560 |
| tissue-specific histochemical localization of iron and ferritin gene expression in transgenic indica rice pusa basmati (oryza sativa l.). | | 2006 | 17072086 |
| a sodium transporter (hkt7) is a candidate for nax1, a gene for salt tolerance in durum wheat. | durum wheat (triticum turgidum subsp. durum) is more salt sensitive than bread wheat (triticum aestivum). a novel source of na(+) exclusion conferring salt tolerance to durum wheat is present in the durum wheat line 149 derived from triticum monococcum c68-101, and a quantitative trait locus contributing to low na(+) concentration in leaf blades, nax1, mapped to chromosome 2al. in this study, we used the rice (oryza sativa) genome sequence and data from the wheat expressed sequence tag deletion ... | 2006 | 17071645 |