| cell type-specific recognition of human metapneumoviruses (hmpvs) by retinoic acid-inducible gene i (rig-i) and tlr7 and viral interference of rig-i ligand recognition by hmpv-b1 phosphoprotein. | human metapneumoviruses (hmpvs) are recently identified paramyxoviridae that contribute to respiratory tract infections in children. no effective treatments or vaccines are available. successful defense against virus infection relies on early detection by germ line-encoded pattern recognition receptors and activation of cytokine and type i ifn genes. recently, the rna helicase retinoic acid-inducible gene i (rig-i) has been shown to sense hmpv. in this study, we investigated the abilities of two ... | 2010 | 20042593 |
| pulmonary infection of mice with human metapneumovirus induces local cytotoxic t-cell and immunoregulatory cytokine responses similar to those seen with human respiratory syncytial virus. | human metapneumovirus (hmpv) is a major cause of upper and lower respiratory-tract infection in infants, the elderly and immunocompromised individuals. virus-directed cellular immunity elicited by hmpv infection is poorly understood, in contrast to the phylogenetically and clinically related pathogen human respiratory syncytial virus (hrsv). in a murine model of acute lower respiratory-tract infection with hmpv, we demonstrate the accumulation of gamma interferon (ifn-gamma)-producing cd8+ t cel ... | 2010 | 20053825 |
| hematopoietic cell transplantation and emerging viral infections. | viral infections remain important causes of morbidity and mortality in hematopoietic cell transplant recipients. more recent developments in preparative regimens and graft manipulations, as well as the control of well-recognized post-transplant infections by the introduction of prophylaxis and preemptive strategies, have influenced the timing and the epidemiology of infections. as new pathogens, such as human metapneumovirus (hmpv), human bocavirus, human coronaviruses hcov-nl63 and hcov-hku1, h ... | 2010 | 20087928 |
| [some considerations about the comparative study of diagnostic techniques for infections due to human metapneumovirus]. | | 2010 | 20097451 |
| [construction of human metapneumovirus dna vaccine and study on its immune response in mice]. | to construct human metapneumovirus (hmpv) dna vaccines and evaluate the cellular and humoral immune response in mice. | 2009 | 20104747 |
| estimates of individuals exposed to human metapneumovirus in a community-based taiwanese population in 1999. | human metapneumovirus (hmpv) causes both upper and lower respiratory tract infection worldwide. because little is known about the estimated level of exposure to hmpv in taiwan, data for 1999 are presented. one thousand one hundred twenty sera from otherwise healthy community-based subjects aged 1 month to 99 years were tested in an enzyme-linked immunosorbent assay; the proportion of seropositive individuals was 68.1%. moreover, 53.2% of preschool children were seronegative, whereas 88.3% of sch ... | 2010 | 20112120 |
| prevalence and clinical and molecular characterization of human metapneumovirus in children with acute respiratory infection in china. | human metapneumovirus (hmpv), a newly discovered paramyxovirus, has been associated with acute respiratory tract infections (artis). however, the prevalence and molecular characteristics of hmpv in china are still unclear. | 2010 | 20135829 |
| [an etiological study on acute respiratory infection among inpatient children in suzhou]. | to investigate the pathogen of children with acute respiratory infection (ari) in suzhou and to provide some evidences for clinical diagnosis and treatment. | 2009 | 20137465 |
| a novel multiplex real-time rt-pcr assay with fret hybridization probes for the detection and quantitation of 13 respiratory viruses. | quantitative multiplex real-time rt-pcr assays utilizing fluorescence resonance energy transfer (fret) hybridization probes were developed for the detection of 13 respiratory viruses, including well recognized viral causes (respiratory syncytial virus, influenza viruses a and b, parainfluenza viruses types 1, 2, and 3, adenovirus) as well as viruses described recently as causes of acute respiratory tract infections (human coronaviruses nl63, hku1, 229e, and oc43, human bocavirus, and human metap ... | 2010 | 20153377 |
| usefulness of two new methods for diagnosing metapneumovirus infections in children. | human metapneumovirus (hmpv) is associated with acute respiratory tract infections, mainly in paediatric patients. the aim of this study was to evaluate the usefulness of two new commercial techniques available for the detection of hmpv in clinical samples from children: an enzyme immunoassay, hmpv eia (biotrin international ltd), and a molecular assay, real-time rt-pcr (pro hmpv real time assay kit; prodesse). a total of 184 nasopharyngeal aspirate specimens from 173 children aged less than 5 y ... | 2010 | 20156218 |
| detection of human metapneumovirus and respiratory syncytial virus by duplex real-time rt-pcr assay in comparison with direct fluorescent assay. | human respiratory syncytial virus (hrsv) and human metapneumovirus (hmpv) are important respiratory pathogens of small children and adults. the present study aimed to design a sensitive real-time rt-pcr assay for the detection of hrsv and hmpv in comparison with direct fluorescent assay (dfa) and to determine the incidence of hmpv and hrsv as causative agents of respiratory infections in a finnish population. for dfa detection of hmpv antigen, four commercial antibodies were evaluated. the duple ... | 2010 | 20156219 |
| detection of new respiratory viruses in hospitalized infants with bronchiolitis: a three-year prospective study. | we have designed a study with the objective of describing the clinical impact of other viruses different from the respiratory syncytial virus (rsv) in hospitalized infants with bronchiolitis. | 2010 | 20163373 |
| [analysis of clinical features of community-acquired pneumonia caused by pediatric respiratory syncytial virus and human metapneumovirus]. | we retrospectively reviewed the background, clinical features, blood tests, and complications in the 720 children seen for acute respiratory tract infection from july 2004 to december 2005. of these, 75 (10.5%) were diagnosed with pneumonia due to respiratory syncytial virus (rsv) and 19 (2.6%) with pneumonia due to human metapneumovirus (hmpv) based on multiplex pcr analysis of nasopharyngeal samples. rsv was pcr-positive mostly in winter, -from november to january-, and hmpv mostly in spring, ... | 2010 | 20170013 |
| clinical and epidemiological comparison of human metapneumovirus and respiratory syncytial virus in seoul, korea, 2003-2008. | human metapneumovirus (hmpv) shares clinical and epidemiological characteristics with well-known respiratory syncytial virus (rsv). the aim of this study was to investigate the clinical and epidemiological differences between hmpv- and rsv-induced wheezing illnesses. a total of 1,008 nasopharyngeal aspirate specimens was collected from 1,008 pediatric patients hospitalized with acute respiratory tract infection at inje university sanggye paik hospital from december 2003 to april 2008, and tested ... | 2010 | 20191030 |
| contribution of human metapneumovirus to influenza-like infections in north greece, 2005-2008. | following its detection in 2001, human metapneumovirus (hmpv) has repeatedly been reported as a respiratory pathogen, especially in children. this study was aimed at determining the proportion of hmpv infections in patients with influenza-like illness (ili) during the three influenza seasons 2005-6, 2006-7, 2007-8 in northern greece. we collected 380 nasopharyngeal swabs or aspirates from ili patients during the winter seasons 2005-2008 and examined them for influenza viruses and hmpv by one-ste ... | 2010 | 20214868 |
| antiviral activity of the green marine alga ulva fasciata on the replication of human metapneumovirus. | we evaluated the antiviral activity of the marine alga, ulva fasciata, collected from rasa beach and forno beach, búzios, rio de janeiro, brazil on the replication of human metapneumovirus (hmpv). the algae extracts were prepared using three different methodologies to compare the activity of different groups of chemical composites obtained through these different methodologies. four out of the six extracts inhibited nearly 100% of viral replication. the results demonstrated that the majority of ... | 2010 | 20305948 |
| [toll-like receptors expression in the lungs of human metapneumovirus infected mice and the effects of polyi:c on viral infection]. | to investigate the expression changes of toll-like receptors (tlr) in the lungs of human metapneumovirus infected balb/c mice, and to explore the effects of polyi:c on viral replication, hmpv-infected group, polyi:c+hmpv group, polyi:c+dmed group and dmem control group were set up for this study. all mice were sacrificed on day 1, 3, 5, 7, 9 and 16 post inoculation. lungs were used for viral titration, pulmonary histopathology and detection of tlrs mrna expression by rt-pcr and real-time pcr. re ... | 2010 | 20329551 |
| phylogenetic analysis of human metapneumovirus from children with acute respiratory infection in yamaguchi, japan, during summer 2009. | | 2010 | 20332580 |
| lambda interferon renders epithelial cells of the respiratory and gastrointestinal tracts resistant to viral infections. | virus-infected cells secrete a broad range of interferons (ifn) which confer resistance to yet uninfected cells by triggering the synthesis of antiviral factors. the relative contributions of the various ifn subtypes to innate immunity against virus infections remain elusive. ifn-alpha, ifn-beta, and other type i ifn molecules signal through a common, universally expressed cell surface receptor, whereas type iii ifn (ifn-lambda) uses a distinct cell-type-specific receptor complex for signaling. ... | 2010 | 20335250 |
| molecular detection and genetic variability of human metapneumovirus in uruguay. | human metapneumovirus (hmpv) has been described as circulating among the uruguayan population at least since 1998 based on serologic evidence. however, no isolation attempts, molecular detection, or genetic studies have been carried out so far in the country. in the present study, molecular detection of circulating hmpv in children hospitalized with acute respiratory tract infection in montevideo-uruguay was carried out by reverse transcription-polymerase chain reaction (rt-pcr) amplification of ... | 2010 | 20336730 |
| etiology of bronchiolitis in a hospitalized pediatric population: prospective multicenter study. | in 2006, bronchiolitis due to adenovirus nosocomial infections resulted in the closure of a pediatric department in northern portugal. | 2010 | 20362492 |
| studies of culture conditions and environmental stability of human metapneumovirus. | human metapneumovirus (hmpv) is a paramyxovirus that is a leading cause of acute respiratory disease. hmpv is difficult to cultivate and limited published data describe the in vitro growth characteristics of the virus and its ability to replicate in different cell lines. stability of hmpv to different temperatures or environmental conditions has not been described. nosocomial infections due to hmpv have been reported, and thus the survival of infectious particles on environmental surfaces is imp ... | 2010 | 20380856 |
| prevalence of viral infection detected by pcr and rt-pcr in patients with acute exacerbation of copd: a systematic review. | viruses are important aetiological agents of acute exacerbation of copd (aecopd). their reported prevalence varies from region to region. this systematic review calculated the prevalence of respiratory viral infections in aecopd. | 2010 | 20415983 |
| a simple approach for preparing real-time pcr positive reaction controls for rare or emerging viruses. | laboratories often have difficulties obtaining positive control material for polymerase chain reaction (pcr) diagnosis of rare or emerging viruses. this is particularly problematic during outbreaks caused by emerging infectious diseases, when delays can impede the public health response. | 2010 | 20417151 |
| soluble recombinant human metapneumovirus g protein is immunogenic but not protective. | human metapneumovirus (hmpv) expresses the major surface glycoproteins f and g. we evaluated the protective efficacy of immunization with g. we generated a recombinant form of g ectodomain (gdeltatm) that was secreted from mammalian cells and purified by affinity chromatography. we tested the immunogenicity of gdeltatm in cotton rats. animals were immunized with pbs, gdeltatm alone or adjuvanted, or were infected once with hmpv, and challenged with live hmpv at 28 days. animals vaccinated with a ... | 2010 | 20417260 |
| human metapneumovirus in hospitalized children in amman, jordan. | human metapneumovirus (hmpv) has recently been identified as an important cause of acute respiratory infections (ari) in children worldwide. however, there is little systematic data on its frequency and importance as a cause of ari in the middle east. we conducted a viral surveillance study in children <5 years of age admitted with respiratory symptoms and/or fever at two major tertiary care hospitals in amman, jordan from 1/18-3/29/07. nose and throat swabs were collected and tested for hmpv an ... | 2010 | 20419816 |
| detection of novel respiratory viruses from influenza-like illness in the philippines. | several novel viruses have been recently identified in respiratory samples. however, the epidemiology of these viruses in tropical countries remains unclear. the aim of the present study was to provide an overview of the epidemiology of novel respiratory viruses, including human metapneumovirus, human bocavirus, new subtypes of human coronavirus (nl63 and hku1), ki virus, wu virus, and melaka virus in the philippines, a tropical country. nasopharyngeal aspirates from 465 patients with influenza- ... | 2010 | 20419824 |
| [detection and clinical characterization of wu polyomavirus in acute respiratory tract infection in children]. | wu polyomavirus (wupyv), a new member of the genus polyomavirus in the family polyomaviridae, has been found to be associated with respiratory tract infections recently. but the role of the wupyv as agents of human disease remains uncertain. we sought to describe the detection and clinical characterization of wupyv in acute respiratory tract infection in children. | 2010 | 20426930 |
| [analysis of viral etiology of severe pneumonia in infants and young children in chongqing area]. | to investigate the prevalence of viral infections and putative association of viral infection with illness severity in young children with severe lower respiratory tract infection (lrti) in chongqing. | 2010 | 20426941 |
| population-based incidence of human metapneumovirus infection among hospitalized children. | human metapneumovirus (hmpv) is a leading cause of acute respiratory illness (ari) in children. population-based incidence rates and comprehensive clinical characterizations of disease have not been established. | 2010 | 20446850 |
| breastfeeding is associated with the production of type i interferon in infants infected with influenza virus. | breast milk-mediated protection against respiratory viruses is well established. however, protective mechanisms are unclear. type i interferons (ifn) mediate host defence against respiratory viruses, particularly influenza virus. the relationship among type i ifn, respiratory viral infections and breastfeeding has not been explored. | 2010 | 20456265 |
| incidence and morbidity of human metapneumovirus and other community-acquired respiratory viruses in lung transplant recipients. | to determine the role of human metapneumovirus (hmpv) in respiratory tract infections (rtis) of lung transplant recipients, 60 patients were prospectively enrolled in this study spanning from september 2005 to november 2007. community-acquired respiratory viruses (carvs) were identified by polymerase chain reaction and tissue culture in respiratory secretions. of 112 rtis, 51 were associated with > or =1 carv, including 7 hmpv, 13 respiratory syncytial virus (rsv), 19 parainfluenza virus 1, 2, o ... | 2010 | 20456714 |
| quantitative proteome profiling of respiratory virus-infected lung epithelial cells. | respiratory virus infections are among the primary causes of morbidity and mortality in humans. influenza virus, respiratory syncytial virus (rsv), parainfluenza (piv) and human metapneumovirus (hmpv) are major causes of respiratory illness in humans. especially young children and the elderly are susceptible to infections with these viruses. in this study we aim to gain detailed insight into the molecular pathogenesis of respiratory virus infections by studying the protein expression profiles of ... | 2010 | 20470912 |
| human metapneumovirus subgroup changes and seasonality during epidemics. | human metapneumovirus (hmpv) is a major cause of respiratory tract illness in young children and causes annual outbreaks in winter and spring seasons. we evaluated the subgroups of hmpv that caused annual outbreaks and its seasonal occurrence during a 21-year period. | 2010 | 20489673 |
| viral etiology of severe pneumonia among kenyan infants and children. | pneumonia is the leading cause of childhood death in sub-saharan africa. comparative estimates of the contribution of causative pathogens to the burden of disease are essential for targeted vaccine development. | 2010 | 20501927 |
| role of emerging respiratory viruses in children with severe acute wheezing. | acute wheezing episodes are frequently associated with respiratory viral infections in children. however, the role of the recently described respiratory viruses is not yet fully understood. | 2010 | 20503284 |
| viruses and atypical bacteria associated with asthma exacerbations in hospitalized children. | objectives and working hypothesis: to evaluate the prevalence of respiratory viruses mycoplasma pneumoniae and chlamydophila pneumoniae and gain insight into their seasonal circulation pattern in children with acute asthma exacerbations in a temperate southern hemisphere region. | 2010 | 20503289 |
| viral respiratory infections in hospitalized and community control children in alaska. | respiratory syncytial virus (rsv) in alaska native children from the yukon kuskokwim (yk) delta is associated with a hospitalization rate five times higher than that reported for the general us child population. the role of other viral respiratory pathogens has not been studied in this population. yk delta children <3 years of age hospitalized with respiratory infections and same aged community control children were prospectively enrolled between october 2005 and september 2007. polymerase chain ... | 2010 | 20513097 |
| [molecular epidemiology of human metapneumovirus in children with acute lower respiratory tract infections in shanghai.]. | to understand the clinical and molecular epidemiologic features of human metapneumovirus (hmpv) in children with acute lower respiratory tract infection (alrti) from oct. 2006 to feb. 2008 in shanghai. | 2010 | 20513294 |
| human metapneumovirus. | respiratory tract infections (rti) are the leading cause of death in low-income countries and the second leading cause of death worldwide in children less than 5 years old. most rti are viral. human metapneumovirus (hmpv) was discovered in 2001 in routine viral cultures of respiratory specimens from children with rti and has been implicated as a common cause of rti in children and adults and a cause of severe disease in immunocompromised hosts. this article describes the microbiology, epidemiolo ... | 2010 | 20513544 |
| severe human metapneumovirus pneumonia in a child with chronic illness. | | 2010 | 20522618 |
| [detection of mycoplasma pneumoniae, chlamydia trachomatis and common respiratory viruses in children with acute respiratory infection in nanjing]. | to investigate the nonbacterial pathogens in children with acute respiratory infection (ari) in nanjing. | 2010 | 20540855 |
| comparison of virus isolation using the vero e6 cell line with real-time rt-pcr assay for the detection of human metapneumovirus. | the use of cell culture for the diagnosis of human metapneumovirus (hmpv) infection is uncommon at present and molecular method such as reverse-transcription pcr (rt-pcr) has been widely and most commonly used as the preferred test. we aimed to compare the results of virus isolation using vero e6 cells with real-time rt-pcr for the detection of hmpv, since such a comparison data is not available. | 2010 | 20546620 |
| [bronchiolitis viruses]. | in normandy (france), human respiratory syncytial virus (hrsv) was detected in 64.1% of acute bronchiolitis in hospitalized children, rhinovirus in 26.8%, human metapneumovirus (hmpv) in 7.6%, and parainfluenza virus (piv) in 3.4%. the viruses causing acute bronchiolitis in the community were hrsv (42%), rhinovirus (19.5%), coronavirus (8%), piv (3.5%), and hmpv (2.5%). in 53.7% of the cases, hrsv infected infants (86.9%), 53.7% being less than 6 months of age. of the hrsv cases, 48.2% were dete ... | 2010 | 20558050 |
| acute lower respiratory tract infections by human metapneumovirus in children in southwest china: a 2-year study. | human metapneumovirus (hmpv) has been reported to cause both upper and lower respiratory tract diseases in susceptible populations, particularly in children and the elderly. in this study, we describe a hospital-based epidemiological study of hmpv in patients presenting to a children's hospital and show the demographic and clinical characteristics associated with hmpv infection in china, retrospectively. specimens were collected over a 2-year period from children hospitalized with acute lower re ... | 2010 | 20583291 |
| the distinguishing features of human metapneumovirus and respiratory syncytial virus. | acute respiratory tract infections (rtis) are a leading cause of morbidity and mortality worldwide. human metapneumovirus (hmpv) is a member of the metapneumovirus genus within the pneumovirinae subfamily of the paramyxoviridae family. though hmpv was only discovered in 2001, a large body of work has already shown that it is the aetiologic agent of a substantial proportion of upper and lower rtis across all age groups in both healthy and immunocompromised hosts throughout the world. rsv, also a ... | 2010 | 20586081 |
| emerging viruses in transplantation. | several viruses have recently gained importance for the transplant recipient. the purpose of this review is to give an update on emerging viruses in transplantation. | 2010 | 20592533 |
| prophylactic and therapeutic benefits of a monoclonal antibody against the fusion protein of human metapneumovirus in a mouse model. | human metapneumovirus (hmpv) is a paramyxovirus causing acute respiratory tract infections in humans. the effects of a monoclonal antibody (mab 338, medimmune, inc.) directed against the hmpv fusion protein were assessed in vivo. different groups of balb/c mice received an intraperitoneal injection of 25 or 50mg/kg of mab 338 either 24h before or 48h after viral infection. lung samples were collected on days 5 and 42 after infection for determination of viral titers and histopathological changes ... | 2010 | 20619294 |
| viral and mycoplasma pneumoniae community-acquired pneumonia and novel clinical outcome evaluation in ambulatory adult patients in china. | few studies have addressed the etiology and clinical outcomes of community-acquired pneumonia (cap) treated in an ambulatory setting. we investigated the etiology by the culture of mycoplasma pneumoniae, urine antigen testing of streptococcus pneumoniae and legionella pneumoniae, and dna or rna determination of eight kinds of respiratory virus dna or rna. an etiological diagnosis was made in 51.8% of 197 patients. the most common pathogens were m. pneumoniae (29.4%) followed by influenza virus a ... | 2010 | 20623362 |
| detection of respiratory viruses and the associated chemokine responses in serious acute respiratory illness. | a specific diagnosis of a lower respiratory viral infection is often difficult despite frequent clinical suspicion. this low diagnostic yield may be improved by use of sensitive detection methods and biomarkers. | 2010 | 20627924 |
| epidemiology of viral respiratory tract infections in a prospective cohort of infants and toddlers attending daycare. | the epidemiology of respiratory tract infections (rtis) in a daycare cohort has not been explored using molecular techniques. | 2010 | 20650679 |
| comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems. | two molecular assays were compared with real-time rt-pcr and viral culture for simultaneous detection of common viruses from respiratory samples: a multiplex ligation-dependant probe amplification (mlpa) and a dual priming oligonucleotide system (dpo). in addition, the positive detections of mlpa and dpo were identified using two different automatic electrophoresis systems. a panel of 168 culture-positive and negative samples was tested by the molecular assays for the presence of influenza a and ... | 2010 | 20691735 |
| measuring the physical cohesiveness of proteins using physical interaction enrichment. | protein-protein interaction (ppi) networks are a valuable resource for the interpretation of genomics data. however, such networks have interaction enrichment biases for proteins that are often studied. these biases skew quantitative results from comparing ppi networks with genomics data. here, we introduce an approach named physical interaction enrichment (pie) to eliminate these biases. | 2010 | 20798171 |
| the synthetic bacterial lipopeptide pam3csk4 modulates respiratory syncytial virus infection independent of tlr activation. | respiratory syncytial virus (rsv) is an important cause of acute respiratory disease in infants, immunocompromised subjects and the elderly. however, it is unclear why most primary rsv infections are associated with relatively mild symptoms, whereas some result in severe lower respiratory tract infections and bronchiolitis. since rsv hospitalization has been associated with respiratory bacterial co-infections, we have tested if bacterial toll-like receptor (tlr) agonists influence rsv-a2-gfp inf ... | 2010 | 20808895 |
| [human metapneumovirus]. | respiratory viral infections are the most significant cause of increased mortality and morbidity especially in immunocompromised people. these infections are increasingly recognized as being the cause of the failure of a graft or the cause of death in both solid organ and hematopoietic stem cell transplant recipients. treatment with potent immunosuppressive medication is necessary for regulation in order to prevent rejection of solid organs and graft-versus-host disease. as a consequence of this ... | 2010 | 20809463 |
| human metapneumovirus genotypes and severity of disease in young children (n = 100) during a 7-year study in dijon hospital, france. | human metapneumovirus is a cause of respiratory tract infections at all ages. our objectives were to analyze the distribution of the a and b genotypes over 7 years in dijon and to investigate a possible association between hmpv genotypes and disease severity. during 2002-2009, we genotyped the 100 isolates from children <3 years old with hmpv. phylogenetic analysis indicated a change in the distribution of hmpv genotype over the years. severity was then measured by detailed clinical evaluation. ... | 2010 | 20827777 |
| noninvasive monitoring of respiratory viruses in wild chimpanzees. | to diagnose respiratory disease among wild great apes, there is a need for noninvasive diagnostic methods. therefore, we analyzed fecal samples from habituated chimpanzees from taï national park, côte d'ivoire. samples had been collected during four distinct outbreaks: two with known aetiology (march 2004 and february 2006) and two with unknown aetiology (october 2004 and august 2005). fecal samples were screened by polymerase chain reaction (pcr) for the presence of human metapneumovirus (hmpv) ... | 2010 | 20865440 |
| molecular detection of respiratory viruses in clinical specimens from children with acute respiratory disease in iran. | viruses are the most common causes of respiratory tract infections in children. the implementation of rapid virology assays can identify the most common pathogens involved. this study was undertaken on iranian children less than 6 years old with respiratory infection. | 2010 | 20879092 |
| longitudinal course of human metapneumovirus antibody titers and reinfection in healthy adults. | the purpose of this study was to evaluate the frequency of human metapneumovirus (hmpv) infection in adults and to determine the association between the levels of serum antibody titers and the susceptibility to reinfection. serum samples collected at the periodic occupational medical checkup for employees of a hospital were subjected to an elisa test. of the 289 subjects, 288 (99.7%) had hmpv antibody titers that were more than 1:100 in may 2006. the percentage of subjects with a titer of ≥ 3,20 ... | 2010 | 20981798 |
| serologically defined human metapneumovirus in pediatric community-acquired pneumonia. | | 2010 | 21030038 |
| evaluation of commercial resplex ii v2.0, multicode-plx, and xtag respiratory viral panels for the diagnosis of respiratory viral infections in adults. | commercial multiplex pcr panels for respiratory viruses (prv) have been recently developed. resplex ii panel v2.0 (qiagen), multicode-plx (eragen biosciences), and xtag (luminex) prv's were studied. all assays detect influenza a and b, adenovirus, parainfluenza 1-3, respiratory syncytial virus a and b, human metapneumovirus and human rhinovirus. the resplex ii additionally detects coronavirus (229e, oc43, nl63, hku1), coxsackie/echo virus, bocavirus and differentiates adenoviruses (b, e). the mu ... | 2010 | 21050809 |
| co-circulation of genetically distinct human metapneumovirus and human bocavirus strains in young children with respiratory tract infections in italy. | the discovery of human metapneumovirus (hmpv) and human bocavirus (hbov) identified the etiological causes of several cases of acute respiratory tract infections in children. this report describes the molecular epidemiology of hmpv and hbov infections observed following viral surveillance of children hospitalized for acute respiratory tract infections in milan, italy. pharyngeal swabs were collected from 240 children ≤3 years of age (130 males, 110 females; median age, 5.0 months; iqr, 2.0-12.5 ... | 2011 | 21108354 |
| integrated capillary electrophoresis microsystem for multiplex analysis of human respiratory viruses. | we developed a two-layer, four-channel polymerase chain reaction (pcr)-capillary electrophoresis microdevice that integrates nucleic acid amplification, sample cleanup and concentration, capillary electrophoretic separation, and detection for multiplex analysis of four human respiratory viral pathogens, influenza a, influenza b, coronavirus oc43, and human metapneumovirus. biotinylated and fluorescently labeled double-stranded (ds) deoxyribonucleic acid (dna) amplification products are generated ... | 2010 | 21114282 |
| simultaneous detection and differentiation of respiratory syncytial virus and other respiratory viral pathogens. | rapid and accurate detection of respiratory syncytial virus (rsv) provides pathogen-specific diagnosis, allows implementation of appropriate infection control measures, and improves patient management. one diagnostic challenge is that respiratory infections, which can be caused by several viral pathogens including rsv, usually present with similar signs and symptoms that are nearly indistinguishable by clinical diagnosis. we have described in the chapter a rapid, high-throughput laboratory techn ... | 2011 | 21116808 |
| effects of human respiratory syncytial virus, metapneumovirus, parainfluenza virus 3 and influenza virus on cd4+ t cell activation by dendritic cells. | background: human respiratory syncytial virus (hrsv), and to a lesser extent human metapneumovirus (hmpv) and human parainfluenza virus type 3 (hpiv3), re-infect symptomatically throughout life without antigenic change, suggestive of incomplete immunity. one causative factor is thought to be viral interference with dendritic cell (dc)-mediated stimulation of cd4+ t cells. methodology, principal findings: we infected human monocyte-derived dc with purified hrsv, hmpv, hpiv3, or influenza a virus ... | 2010 | 21124776 |
| prevalence of human metapneumovirus (hmpv) in children with wheezing in shiraz-iran. | human metapneumovirus (hmpv) infection plays an important role in the pediatric respiratory infections. the aim of this study was to determine the relationship between asthma and wheezing with hmpv in hospitalized children. nasal pharyngeal swabs obtained from 120 children aged 1-60 months, hospitalized during a one year period, were tested for the hmpv by rt-pcr. hmpv was detected in 20 (16.6%) of patients suffering from wheezing. some patients in addition to wheezing had asthma 10.8%. this inf ... | 2010 | 21131706 |
| endemicity of human metapneumovirus subgenogroups a2 and b2 in yamagata, japan, between 2004 and 2009. | to clarify a longitudinal epidemiology,we isolated 280 hmpv strains from patients with acute respiratory infections in yamagata, japan, between 2004 and 2009.we observed that the high season for hmpv was from winter to spring (between january and may) and the low season was in the fall (around september and october). a further molecular analysis revealed that subgenogroup a2 (a2) strains were the most commonly isolated (151/280; 53.9%), followed by b2 (108/280; 38.6%) and b1 (19/280; 6.8%). our ... | 2010 | 21140598 |
| molecular virology in the clinical laboratory. | as one can see by the tests listed at www.amp.org, molecular diagnostic techniques have enabled the laboratory professionals to play an integral role in the identification and quantitation of viral infectious agents. viral loads can be determined for hiv, hbv, and hcv using a variety of molecular methods such as real-time pcr, tma, nasba, and bdna. determining the amount of viral particles in a sample can not only monitor the status and progression of the disease, but can also guide recommendati ... | 2010 | 21140798 |
| the changing face of pediatric respiratory tract infections: how human metapneumovirus and human bocavirus fit into the overall etiology of respiratory tract infections in young children. | lower respiratory tract infections are one of the leading causes of morbidity and mortality in children worldwide. recent technological advances in the field of molecular biology have allowed virologists to detect many previously undetected viral pathogens. two of these, human metapneumovirus (hmpv) and human bocavirus (hbov), are of particular clinical interest to pediatric health care providers. this review discusses the most common viral respiratory infections in children, explores the role o ... | 2010 | 21165338 |
| [human metapneumoviruses were isolated from infants and children with acute respiratory infections in beijing]. | to obtain isolated human metapneumovirus (hmpv) strains from clinical specimens collected from infants and children in beijing and to promote the investigation on this important respiratory pathogen. | 2010 | 21215024 |
| impact of human metapneumovirus infection on in and outpatients for the years 2006-2008 in southern brazil. | the human metapneumovirus (hmpv), member of the paramyxoviridae family, has been reported as an important agent involved with acute respiratory infections (aris). the aim of this study is to identify hmpv as the etiological agent of aris on in and outpatients in the city of curitiba, southern brazil, and describe clinical data of hmpv subtyping. a retrospective study was performed in 1,572 respiratory samples over a period of three years. hmpv was detected by reverse transcription-polymerase cha ... | 2010 | 21225198 |
| the cellular endosomal sorting complex required for transport pathway is not involved in avian metapneumovirus budding in a virus-like-particle expression system. | avian metapneumovirus (ampv) is a paramyxovirus that principally causes respiratory disease and egg production drops in turkeys and chickens. together with its closely related human metapneumovirus (hmpv), they comprise the genus metapneumovirus in the family paramyxoviridae. little is currently known about the mechanisms involved in the budding of metapneumovirus. by using ampv as a model system, we showed that the matrix (m) protein by itself was insufficient to form virus-like-particles (vlps ... | 2011 | 21248175 |
| association between human metapneumovirus seroprevalence and hypertension in elderly subjects in a long-term care facility. | recently, relations between hypertension and infections caused by several pathogens have been reported. however, few studies have examined the relationship between human metapneumovirus (hmpv) and hypertension in elderly inpatients. to assess the association between anti-hmpv-immunoglobulin g (igg) titer and the prevalence of hypertension, we conducted a case-control study in a japanese long-term care facility (ltcf). the participants included 84 hypertensive patients aged 65 years, and 84 age- ... | 2011 | 21248755 |
| reverse transcription polymerase chain reaction and electrospray ionization mass spectrometry for identifying acute viral upper respiratory tract infections. | diagnosis of respiratory viruses traditionally relies on culture or antigen detection. we aimed to demonstrate capacity of the reverse transcription polymerase chain reaction/electrospray ionization mass spectrometry (rt-pcr/esi-ms) platform to identify clinical relevant respiratory viruses in nasopharyngeal aspirate (npa) samples and compare the diagnostic performance characteristics relative to conventional culture- and antigen-based methods. an rt-pcr/esi-ms respiratory virus surveillance kit ... | 2011 | 21251562 |
| rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by rt-pcr and electrospray ionization mass spectrometry. | diagnosis of the etiologic agent of respiratory viral infection relies traditionally on culture or antigen detection. this pilot evaluation compared performance characteristics of the rt-pcr and electrospray ionization mass spectrometry (rt-pcr/esi-ms) platform to conventional virologic methods for identifying multiple clinically relevant respiratory viruses in nasopharyngeal aspirates. the rt-pcr/esi-ms respiratory virus surveillance kit was designed to detect respiratory syncytial virus, influ ... | 2011 | 21256867 |
| epidemiological and phylogenic study of human metapneumovirus infections during three consecutive outbreaks in normandy, france. | human metapneumovirus (hmpv) is responsible for respiratory tract disease, particularly in the young and elderly population. an epidemiological and phylogenic study was performed on children admitted to hospital with an acute lower respiratory tract infection (lri). data were obtained and analyzed over three consecutive winters, from 2002-2003 to 2004-2005. each year during the winter period, from november to march, 2,415 nasal swabs were tested by a direct immunofluorescence assay (dfa) for inf ... | 2011 | 21264874 |
| detection of human metapneumovirus genomes during an outbreak of bronchitis and pneumonia in a geriatric care home in shimane, japan, in autumn 2009. | | 2011 | 21266764 |
| virus-related lower respiratory inflammation: detection of human metapneumovirus from severe pneumonia cases with a/h1n1pdm virus. | | 2011 | 21266766 |
| human metapneumovirus infection as an emerging pathogen causing acute respiratory distress syndrome. | | 2011 | 21288832 |
| genetic variability of human metapneumovirus amongst an all ages population in cambodia between 2007 and 2009. | first identified in 2001, human metapneumovirus (hmpv) is a novel pathogen and causative agent of acute respiratory tract infection. re-infection with hmpv is common, and currently there is no available vaccine against hmpv infection. two genotypes of hmpv have been identified, a and b, both of which can be divided further into at least two distinct sub-genotypes. here we report the results of the first study to investigate the genetic variability of hmpv strains circulating within cambodia. the ... | 2011 | 21292032 |
| respiratory infections by hmpv and rsv are clinically indistinguishable but induce different host response in aged individuals. | human metapneumovirus and respiratory syncytial virus can cause severe respiratory diseases, especially in infants, young children, and the elderly. so far it remains unclear why infections in the elderly become life threatening despite the presence of neutralizing antibodies in the serum, and to which extent double infections worsen the clinical course. | 2011 | 21298115 |
| twelve years' detection of respiratory viruses by immunofluorescence in hospitalised children: impact of the introduction of a new respiratory picornavirus assay. | direct immunofluorescence assays (dfa) are a rapid and inexpensive method for the detection of respiratory viruses and may therefore be used for surveillance. few epidemiological studies have been published based solely on dfa and none included respiratory picornaviruses and human metapneumovirus (hmpv). we wished to evaluate the use of dfa for epidemiological studies with a long-term observation of respiratory viruses that includes both respiratory picornaviruses and hmpv. | 2011 | 21299840 |
| using the full spectral capacity (six channels) of a real-time pcr instrument can simplify diagnostic laboratory screening and typing protocols for pandemic h1n1 influenza. | timely reporting of influenza a virus subtype affects patient management. real-time pcr is a rapid and sensitive method routinely used to characterise viral nucleic acid, but the full spectral capability of the instruments is not employed. | 2010 | 21306574 |
| influenza and other respiratory viruses in three central american countries. | despite the disease burden imposed by respiratory diseases on children in central america, there is a paucity of data describing the etiologic agents of the disease. | 2010 | 21306576 |
| large-scale seroprevalence analysis of human metapneumovirus and human respiratory syncytial virus infections in beijing, china. | human metapneumovirus (hmpv), a recently identified virus, causes acute respiratory tract infections (artis) in infants and children. however, studies on the seroepidemeology of hmpv are very limited in china. to assess the seroprevalence of hmpv infection in china, we tested a total of 1,156 serum specimens for the presence of anti-hmpv igg antibody in children and adults free of acute respiratory illness in beijing, china by using hmpv nucleocapsid (n) protein as an antigen. as a control, we u ... | 2011 | 21310026 |
| genetic variability of attachment (g) and fusion (f) protein genes of human metapneumovirus strains circulating during 2006-2009 in kolkata, eastern india. | human metapneumovirus (hmpv) is associated with the acute respiratory tract infection (arti) in all the age groups. however, there is limited information on prevalence and genetic diversity of human metapneumovirus (hmpv) strains circulating in india. | 2011 | 21314961 |
| evidence that tmprss2 activates the severe acute respiratory syndrome coronavirus spike protein for membrane fusion and reduces viral control by the humoral immune response. | the spike (s) protein of the severe acute respiratory syndrome coronavirus (sars-cov) can be proteolytically activated by cathepsins b and l upon viral uptake into target cell endosomes. in contrast, it is largely unknown whether host cell proteases located in the secretory pathway of infected cells and/or on the surface of target cells can cleave sars s. we along with others could previously show that the type ii transmembrane protease tmprss2 activates the influenza virus hemagglutinin and the ... | 2011 | 21325420 |
| detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern brazil. | the introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hmpv) and human bocavirus (hbov), in respiratory tract specimens. we have studied the occurrence of hmpv and hbov in the porto alegre (pa) metropolitan area, one of the southernmost cities of brazil, evaluating children with suspected lower respiratory tract infection from may 2007-june 2008. a real-time polymerase chain reaction method was used for amplification and de ... | 2011 | 21340356 |
| viral etiology among the elderly presenting acute respiratory infection during the influenza season. | acute respiratory tract infections are the most common illness in all individuals. rhinoviruses have been reported as the etiology of more than 50% of respiratory tract infections worldwide. the study prospectively evaluated 47 elderly individuals from a group of 384 randomly assigned for acute respiratory viral infections (cold or flu) and assessed the occurrence of human rhinovirus (hrv), influenza a and b, respiratory syncytial virus and metapneumovirus (hmpv) in botucatu, state of são paulo, ... | 2011 | 21340401 |
| emerging paramyxoviruses: molecular mechanisms and antiviral strategies. | in recent years, several paramyxoviruses have emerged to infect humans, including previously unidentified zoonoses. hendra and nipah viruses (henipaviruses within this family) were first identified in the 1990s in australia, malaysia and singapore, causing epidemics with high mortality and morbidity rates in affected animals and humans. other paramyxoviruses, such as menangle virus, tioman virus, human metapneumovirus and avian paramyxovirus 1, which cause less morbidity in humans, have also bee ... | 2011 | 21345285 |
| [a single-center prospective study of viral infection in community-acquired pneumonia in adults]. | from july, 2008 to march, 2009, 125 adults with community-acquired pneumonia (cap) who were admitted to our hospital were retrospectively investigated to elucidate the characteristics of viral infection in adult cap in japan. nasopharyngeal swabs for real-time polymerase chain reaction for 7 types of influenza virus, rhinovirus, respiratory synctial virus, human metapneumovirus, parainfluenza virus, coronavirus, and enterovirus were obtained. diagnoses of viral infections were established accord ... | 2011 | 21384676 |
| molecular epidemiology and evolution of human respiratory syncytial virus and human metapneumovirus. | human respiratory syncytial virus (hrsv) and human metapneumovirus (hmpv) are ubiquitous respiratory pathogens of the pneumovirinae subfamily of the paramyxoviridae. two major surface antigens are expressed by both viruses; the highly conserved fusion (f) protein, and the extremely diverse attachment (g) glycoprotein. both viruses comprise two genetic groups, a and b. circulation frequencies of the two genetic groups fluctuate for both viruses, giving rise to frequently observed switching of the ... | 2011 | 21390255 |
| human rhinovirus infection in young african children with acute wheezing. | infections caused by human rhinoviruses (hrvs) are important triggers of wheezing in young children. wheezy illness has increasingly been recognised as an important cause of morbidity in african children, but there is little information on the contribution of hrv to this. the aim of this study was to determine the role of hrv as a cause of acute wheezing in south african children. | 2011 | 21401965 |
| bordetella pertussis and concomitant viral respiratory tract infections are rare in children with cough illness. | case reports, case series, and retrospective and prospective studies have reported concomitant bordetella pertussis and viral respiratory tract pathogen infections in children with cough illness with conflicting results regarding their frequency. | 2011 | 21407144 |
| comparison of the luminex xtag respiratory viral panel with xtag respiratory viral panel fast for diagnosis of respiratory virus infections. | nucleic acid tests are sensitive and specific and provide a rapid diagnosis, making them invaluable for patient and outbreak management. multiplex pcr assays have additional advantages in providing an economical and comprehensive panel for many common respiratory viruses. previous reports have shown the utility of the xtag respiratory viral panel (rvp) assay manufactured by luminex molecular diagnostics for this purpose. a newer generation of this kit, released in canada in early 2010, is design ... | 2011 | 21411570 |
| long-term impairment of streptococcus pneumoniae lung clearance is observed after initial infection with influenza a virus but not human metapneumovirus in mice. | human metapneumovirus (hmpv) is a paramyxovirus responsible for respiratory tract infections in humans. our objective was to investigate whether hmpv could predispose to long-term bacterial susceptibility, such as previously observed with influenza viruses. balb/c mice were infected with hmpv or influenza a and, 14 days following viral infection, challenged with streptococcus pneumoniae. only mice previously infected with influenza a demonstrated an 8% weight loss of their body weight 72 h follo ... | 2011 | 21411678 |
| the human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro. | each year, during winter months, human metapneumovirus (hmpv) is associated with epidemics of bronchiolitis resulting in the hospitalization of many infants. bronchiolitis is an acute illness of the lower respiratory tract with a consequent inflammation of the bronchioles. the rapid onset of inflammation suggests the innate immune response may have a role to play in the pathogenesis of this hmpv infection. since, the matrix protein is one of the most abundant proteins in the paramyxoviridae fami ... | 2011 | 21412439 |
| adult outpatient experience of the 2009 h1n1 pandemic: clinical course, pathogens, and evaluation of case definitions. | the aim was to describe causative agents and clinical characteristics in adult outpatients with upper airway symptoms during the 2009 h1n1 pandemic and to evaluate case definitions that are used in clinical practice. | 2011 | 21420427 |
| viral pneumonia. | about 200 million cases of viral community-acquired pneumonia occur every year-100 million in children and 100 million in adults. molecular diagnostic tests have greatly increased our understanding of the role of viruses in pneumonia, and findings indicate that the incidence of viral pneumonia has been underestimated. in children, respiratory syncytial virus, rhinovirus, human metapneumovirus, human bocavirus, and parainfluenza viruses are the agents identified most frequently in both developed ... | 2011 | 21435708 |
| atypical bacterial pathogen infection in children with acute bronchiolitis in northeast thailand. | atypical bacterial pathogens--including mycoplasma pneumoniae, chlamydophila pneumoniae, and chlamydia trachomatis--are important infectious agents of the respiratory system. most current information pertains to adults and little is known about the role of these organisms in lower respiratory tract infections among young children with acute bronchiolitis. | 2011 | 21439510 |