| the expanding targetome of small rnas in salmonella typhimurium. | the enterobacterial pathogen salmonella has long served as a model for bacterial pathogenesis, stress response, gene expression and regulation with extensive investigation involving protein function. with the advent of high-throughput sequencing technologies and their applications in genomics and transcriptomics, a wealth of data particularly with respect to small rnas (srnas) is being generated. these molecules serve as regulators of major stress response and virulence networks in diverse speci ... | 2017 | 28302471 |
| higher storage temperature causes greater salmonella enterica serovar typhimurium internal penetration of artificially contaminated, commercially available, washed free range eggs. | foodborne salmonellosis is a major public health concern, with contaminated eggs identified as a significant source of infection. in australia, the most prevalent cause of salmonellosis from eggs is salmonella enterica subsp. enterica serovar typhimurium. this study explored the effect of temperature after 1, 7, 14, 21, and 28 days of storage on commercially available washed free range eggs, artificially contaminated with salmonella typhimurium on the external surface. at each time point, the ex ... | 2016 | 27357046 |
| antimicrobial resistance and class 1 integron-associated gene cassettes in salmonella enterica serovar typhimurium isolated from pigs at slaughter and abattoir environment. | forty-five multi-resistant salmonella enterica subsp. enterica serovar (s.) typhimurium isolates obtained at five pig abattoirs in southern brazil were characterized. their relatedness was determined by xbai-macrorestriction analysis. resistance genes, integrons and plasmid-mediated quinolone resistance genes (pmqr) were investigated by pcr. amplicons for the variable part of class 1 integrons and the quinolone resistance-determining regions (qrdr) were sequenced. plasmids were characterized by ... | 2016 | 27142182 |
| complete genome sequence of a human-invasive salmonella enterica serovar typhimurium strain of the emerging sequence type 213 harboring a multidrug resistance inca/c plasmid and a blacmy-2-carrying incf plasmid. | salmonella enterica subsp. enterica serovar typhimurium strain 33676 was isolated in mexico city, mexico, from a patient with a systemic infection, and its complete genome sequence was determined using pacbio single-molecule real-time technology. strain 33676 harbors an incf plasmid carrying the extended-spectrum cephalosporin gene blacmy-2 and a multidrug resistance inca/c plasmid. | 2015 | 26564044 |
| a multiplex oligonucleotide ligation-pcr as a complementary tool for subtyping of salmonella typhimurium. | subtyping below the serovar level is essential for surveillance and outbreak detection and investigation of salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) and its monophasic variant 1,4,[5],12:i:- (s. 1,4,[5],12:i:-), frequent causes of foodborne infections. in an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation-pcr (mol-pcr) assay was developed which combines different types of molecular markers ... | 2015 | 26205523 |
| complete genome sequencing of a multidrug-resistant and human-invasive salmonella enterica serovar typhimurium strain of the emerging sequence type 213 genotype. | salmonella enterica subsp. enterica serovar typhimurium strain yu39 was isolated in 2005 in the state of yucatán, mexico, from a human systemic infection. the yu39 strain is representative of the multidrug-resistant emergent sequence type 213 (st213) genotype. the yu39 complete genome is composed of a chromosome and seven plasmids. | 2015 | 26089426 |
| the anti-infective activity of punicalagin against salmonella enterica subsp. enterica serovar typhimurium in mice. | punicalagin, a major bioactive component of pomegranate peel, has been proven to have antioxidant, antiviral, anti-apoptosis, and hepatoprotective properties. the aim of this study was to investigate the anti-infective activity of punicalagin in a mouse model. c57bl/6 mice were initially challenged with salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) and then treated with punicalagin. food and water consumption and body weight were recorded daily. on day 8 post infection ... | 2015 | 26084785 |
| characterization of salmonella enterica serovar typhimurium and salmonella enterica serovar 4,[5],12:i:- isolates from pigs presenting with diarrhea in korea. | between 2011 and 2012, a total of 896 pig fecal samples were collected from nine provinces in korea, and 50 salmonella enterica susp. enterica serovar typhimurium (s. typhimurium) was isolated. the characteristics of the 50 strains were analyzed, and 4 strains were identified as salmonella enterica subsp. enterica serovar 4,[5],12:i:-. salmonella 4,[5],12:i:- could not be distinguished from s. typhimurium through phage typing, antimicrobial resistance testing or multiple-locus variable-number ta ... | 2015 | 26074410 |
| salmonellae interactions with host processes. | salmonellae invasion and intracellular replication within host cells result in a range of diseases, including gastroenteritis, bacteraemia, enteric fever and focal infections. in recent years, considerable progress has been made in our understanding of the molecular mechanisms that salmonellae use to alter host cell physiology; through the delivery of effector proteins with specific activities and through the modulation of defence and stress response pathways. in this review, we summarize our cu ... | 2015 | 25749450 |
| salmonella spp. as a cause of mortality and clinical symptoms in free-living garden bird species in poland. | some species of garden birds are considered to be sensitive to salmonella (s.) spp. infections. the aim of this study was to determine the cause of mortality of six free-living birds in one private property in suburban area of wrocław (poland). in 2013 poland experienced prolonged winter, with low temperatures and snow precipitations. during march and april, two dead individuals of the eurasian siskin (carduelis spinus) and four dead individuals of the greenfinch (carduelis chloris) were found i ... | 2014 | 25638990 |
| invasive salmonella typhimurium st313 with naturally attenuated flagellin elicits reduced inflammation and replicates within macrophages. | invasive non-typhoidal salmonella (ints) are an important cause of septicemia in children under the age of five years in sub-saharan africa. a novel genotype of salmonella enterica subsp. enterica serovar typhimurium (multi-locus sequence type [st] 313) circulating in this geographic region is genetically different to from s. typhimurium st19 strains that are common throughout the rest of the world. s. typhimurium st313 strains have acquired pseudogenes and genetic deletions and appear to be evo ... | 2015 | 25569606 |
| first complete genome sequence of salmonella enterica subsp. enterica serovar typhimurium strain atcc 13311 (nctc 74), a reference strain of multidrug resistance, as achieved by use of pacbio single-molecule real-time technology. | we report the first complete genomic sequence of salmonella enterica subsp. enterica serovar typhimurium strain atcc 13311, the leading food-borne pathogen and a reference strain used in drug resistance studies. de novo assembly with pacbio sequencing completed its chromosome and one plasmid. they will accelerate the investigation into multidrug resistance in salmonella typhimurium. | 2014 | 25278532 |
| association between indoor environmental contamination by salmonella enterica and contamination of eggs on layer farms. | this study involves longitudinal and point-in-time surveys of salmonella carriage and environmental contamination on two commercial cage layer farms positive for salmonella enterica subsp. enterica serovar typhimurium (flock a age, 32 weeks; flock b age, 34 weeks). salmonella-positive fecal, egg belt, and dust samples were all unconditionally associated with eggshells testing positive for salmonella. the odds of an eggshell testing positive for salmonella were 91.8, 61.5, and 18.2 times higher w ... | 2014 | 24966362 |
| immunochromatographic strip assay for the rapid and sensitive detection of salmonella typhimurium in artificially contaminated tomato samples. | this study was designed to confirm the applicability of a liposome-based immunochromatographic assay for the rapid detection of salmonella enterica subsp. enterica serovar typhimurium (salmonella typhimurium) in artificially contaminated tomato samples. to determine the detection limit and pre-enrichment incubation time (10, 12, and 18 h pre-enrichment in 1% buffered peptone water), the tests were performed with different cell numbers of salmonella typhimurium (3 × 10(0), 3 × 10(1), 3 × 10(2), a ... | 2014 | 24896193 |
| diversity of plasmids encoding virulence and resistance functions in salmonella enterica subsp. enterica serovar typhimurium monophasic variant 4,[5],12:i:- strains circulating in europe. | plasmids encoding resistance and virulence properties in multidrug resistant (mdr) salmonella enterica (s.) serovar typhimurium monophasic variant 4,[5],12:i:- isolates recovered from pigs and humans (2006-2008) in europe were characterised. the isolates were selected based on the detection by pcr-amplification of s. typhimurium virulence plasmid pslt genes and were analysed by multi-locus sequence typing (mlst). the resistance genes present in the isolates and the association of these genes wit ... | 2014 | 24586926 |
| effects of leachate from crumb rubber and zinc in green roofs on the survival, growth, and resistance characteristics of salmonella enterica subsp. enterica serovar typhimurium. | the use of green roofs is a growing practice worldwide, particularly in densely populated areas. in an attempt to find new methods for recycling crumb rubber, incorporation of crumb rubber into artificial medium for plant growth in green roofs and similar engineered environments has become an attractive option for the recycling of waste tires. though this approach decreases waste in landfills, there are concerns about the leaching of zinc and other heavy metals, as well as nutrient and organic c ... | 2014 | 24584242 |
| distribution of gifsy-3 and of variants of st64b and gifsy-1 prophages amongst salmonella enterica serovar typhimurium isolates: evidence that combinations of prophages promote clonality. | salmonella isolates harbour a range of resident prophages which can influence their virulence and ability to compete and survive in their environment. phage gene profiling of a range of phage types of salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) indicates a significant level of correlation of phage gene profile with phage type as well as correlation with genotypes determined by a combination of multi-locus variable-number tandem repeat (vntr) typing and clustered regu ... | 2014 | 24475087 |
| mlva as a tool for public health surveillance of human salmonella typhimurium: prospective study in belgium and evaluation of mlva loci stability. | surveillance of salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (mlva), which allow earlier detection and confinement of outbreaks. here, a surveillance study, including phage typing, antimicrobial susceptibility testing and the in europe most commonly used 5-loci mlva on 1,420 s. typhimurium isolates collected between 2010 and 2012 in belgium, was ... | 2013 | 24391880 |
| functional insight from the tetratricopeptide repeat-like motifs of the type iii secretion chaperone sica in salmonella enterica serovar typhimurium. | sica functions both as a class ii chaperone for sipb and sipc of the type iii secretion system (t3ss)-1 and as a transcriptional cofactor for the arac-type transcription factor invf in salmonella enterica subsp. enterica serovar typhimurium. bioinformatic analysis has predicted that sica possesses three tetratricopeptide repeat (tpr)-like motifs, which are important for protein-protein interactions and serve as multiprotein complex mediators. to investigate whether the tpr-like motifs in sica ar ... | 2014 | 24224875 |
| genetic types, gene repertoire, and evolution of isolates of the salmonella enterica serovar 4,5,12:i:- spanish clone assigned to different phage types. | salmonella enterica subsp. enterica 4,[5],12:i:- is one of the most prevalent serovars associated with human infections worldwide. two multidrug-resistant clones, designated spanish and european clones, are recognized as having importance for public health and are subject to control measures in the european union. in this study, 23 clinical isolates belonging to the spanish clone were characterized by multilocus sequence typing, multiple-locus variable number tandem repeat analysis (mlva), pcr a ... | 2013 | 23325816 |
| comparison of heat stress responses of immobilized and planktonic salmonella enterica serovar typhimurium. | salmonellae are often present as immobilized cells in food products in the form of micro-colonies. despite this, most research into salmonella physiology has been performed with bacteria grown as planktonic cultures. in the current study, we compared the transcriptome of planktonic and immobilized salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) subjected to 30 min of heat stress at 45 °c. the expression of 538 genes was differently regulated between the two conditions af ... | 2013 | 23200655 |
| epidemic of salmonellosis in passerine birds in switzerland with spillover to domestic cats. | a die-off of passerine birds, mostly eurasian siskins (carduelis spinus), occurred in multiple areas of switzerland between february and march 2010. several of the dead birds were submitted for full necropsy. bacteriological examination was carried out on multiple tissues of each bird. at gross examination, common findings were light-tan nodules, 1 to 4 mm in diameter, scattered through the esophagus/crop. histologically, a necroulcerative transmural esophagitis/ingluvitis was observed. bacteria ... | 2013 | 23125146 |
| occurrence and characterization of monophasic salmonella enterica serovar typhimurium (1,4,[5],12:i:-) of non-human origin in poland. | the epidemiological role of monophasic salmonella enterica subsp. enterica serovar typhimurium tends to increase, indicating pandemic spread. the aim of the present study was to confirm the occurrence of this serological variant in poland and to report the first cases in belarus and ukraine. genetic similarity of monophasic isolates with salmonella typhimurium already present in these countries was assessed. serotyping, duplex-polymerase chain reaction (pcr) assay, antibiotic resistance and puls ... | 2012 | 23009171 |
| electrochemical detection of salmonella using gold nanoparticles. | a disposable immunosensor for salmonella enterica subsp. enterica serovar typhimurium lt2 (s) detection using a magneto-immunoassay and gold nanoparticles (aunps) as label for electrochemical detection is developed. the immunosensor is based on the use of a screen-printed carbon electrode (spce) that incorporates a permanent magnet underneath. salmonella containing samples (i.e. skimmed milk) have been tested by using anti-salmonella magnetic beads (mbs-psab) as capture phase and sandwiching aft ... | 2013 | 22884647 |
| comparative genomics of australian and international isolates of salmonella typhimurium: correlation of core genome evolution with crispr and prophage profiles. | salmonella enterica subsp enterica serovar typhimurium (s. typhimurium) is a serovar with broad host range. to determine the genomic diversity of s. typhimurium, we sequenced 39 isolates (37 australian and 2 uk isolates) representing 14 repeats groups (rgs) determined primarily by clustered regularly interspaced short palindromic repeats (crispr). analysis of single nucleotide polymorphisms (snps) among the 39 isolates yielded an average of 1,232 snps per isolate, ranging from 128 snps to 11,339 ... | 2017 | 28851865 |
| whole-genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains tt6675 and tt9097 employed in the isolation and characterization of a giant phage mutant collection. | we report here the genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains tt6675 and tt9097, which we utilize for genetic analyses of giant bacterial viruses. our analyses identified several genetic variations between the two strains, most significantly confirming strain tt6675 as a serine suppressor and tt9097 as a nonsuppressor. | 2017 | 28839032 |
| use of multiple-locus variable-number of tandem repeats analysis (mlva) to investigate genetic diversity of salmonella enterica subsp. enterica serovar typhimurium isolates from human, food, and veterinary sources. | salmonella enterica subspecies enterica serovar typhimurium is the most common zoonotic pathogen in bulgaria. to allow efficient outbreak investigations and surveillance in the food chain, accurate and discriminatory methods for typing are needed. this study evaluated the use of multiple-locus variable-number of tandem repeats analysis (mlva) and compared results with antimicrobial resistance (amr) determinations for 100 s. typhimurium strains isolated in bulgaria during 2008-2012 (50 veterinary ... | 2017 | 28836358 |
| genome sequence of an australian monophasic salmonella enterica subsp. enterica typhimurium isolate (tw-stm6) carrying a large plasmid with multiple antimicrobial resistance genes. | we report the genome sequence of a monophasic salmonella enterica subsp. enterica typhimurium strain (tw-stm6) isolated in australia that is similar to epidemic multidrug-resistant strains from europe and elsewhere. this strain carries additional antibiotic and heavy-metal resistance genes on a large (275-kb) inchi2 plasmid. | 2017 | 28818900 |
| ground water as the source of an outbreak of salmonella enteritidis. | in september 2014, an outbreak of gastroenteritis was reported to the public health institute of šibenik and knin county in croatia. the outbreak occurred in the county center of šibenik, a town with 50,000 inhabitants, and it lasted for 12days. an epidemiological investigation suggested a nearby water spring as the source of the outbreak. due to the temporary closure of the public water supply system, the inhabitants started to use untreated water from a nearby spring. microbiological analysis ... | 2017 | 28756827 |