| a recombinant potato virus y infectious clone tagged with the rosea1 visual marker (pvy-ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants. | potato virus y (pvy) is a major threat to the cultivation of potato and other solanaceous plants. by inserting a cdna coding for the antirrhinum majus rosea1 transcription factor into a pvy infectious clone, we created a biotechnological tool (pvy-ros1) that allows infection by this relevant plant virus to be tracked by the naked eye with no need for complex instrumentation. rosea1 is an myb-type transcription factor whose expression activates the biosynthesis of anthocyanin pigments in a dose-s ... | 2017 | 28428782 |
| identification of mapks as signal transduction components required for the cell death response during compatible infection by the synergistic pair potato virus x-potato virus y. | systemic necrosis is one of the most severe symptoms caused in compatible plant-virus interactions and shares common features with the hypersensitive response (hr). mitogen-activated protein kinase (mapk) cascades are associated with responses to compatible and incompatible host-virus interactions. here, we show that virus-induced gene silencing of the nicotiana benthamiana mapk genes salicylic acid-induced protein kinase (sipk) and wound-induced protein kinase (wipk), and the mapk kinase (mapkk ... | 2017 | 28647505 |
| a novel recombined potato virus y isolate in china. | this study reports the findings of a distinct potato virus y (pvy) isolate found in northeast china. one hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of harbin in heilongjiang province of china. the collected tubers were planted and let to grow in a greenhouse. new potato plants generated from these tubers showed similar symptoms, except for one plant. subsequent serological analyses revealed pvy as the causing agent of the ... | 2017 | 28811755 |
| breeding of a new potato variety 'nagasaki kogane' with high eating quality, high carotenoid content, and resistance to diseases and pests. | 'nagasaki kogane' is a new potato variety bred from a cross between 'saikai 35' as a female parent and 'saikai 33' as a male. 'saikai 35' is resistant to bacterial wilt, contains the h1 and rychc genes for resistance to the potato cyst nematode (pcn) and potato virus y (pvy), respectively, and has high carotenoid content, while 'saikai 33' has large and high-yielding tubers and is resistant to both bacterial wilt and pcn. the carotenoid content of 'nagasaki kogane' is higher than that of 'dejima ... | 2017 | 28744186 |
| silencing the tobacco gene for rna-dependent rna polymerase 1 and infection by potato virus y cause remodeling of cellular organelles. | rna-dependent rna polymerase 1 (rdr1) has been shown to be involved in dna methylation, rna silencing and regulating expression of other genes. rdr1 gene expression is stimulated by infection with potato virus y° (pvy). transgenic nicotiana tabacum plants silenced for rdr1 gene expression showed morphological changes in mesophyll cells, associated with remodeling of the nuclei, chloroplasts and mitochondria. rdr1 silencing led to decreased nuclear size, increased heterochromatin content and aggr ... | 2017 | 28719835 |
| the effects of potato virus y-derived virus small interfering rnas of three biologically distinct strains on potato (solanum tuberosum) transcriptome. | potato virus y (pvy) is one of the most economically important pathogen of potato that is present as biologically distinct strains. the virus-derived small interfering rnas (vsirnas) from potato cv. russet burbank individually infected with pvy-n, pvy-ntn and pvy-o strains were recently characterized. plant defense rna-silencing mechanisms deployed against viruses produce vsirnas to degrade homologous viral transcripts. based on sequence complementarity, the vsirnas can potentially degrade host ... | 2017 | 28716126 |
| integrated analysis of tobacco mirna and mrna expression profiles under pvy infection provids insight into tobacco-pvy interactions. | potato virus y (pvy) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.to explore the molecular pvy-host interactions, we analysed changes in the mirna and mrna profiles of tobacco in response to pvy infection. a total of 81 differentially expressed mirnas belonging to 29 families and 8133 mrnas were identified. the gene ontology (go) enrichment analyses showed that genes encoding the ... | 2017 | 28687775 |
| comparison between proteome and transcriptome response in potato (solanum tuberosum l.) leaves following potato virus y (pvy) infection. | plant diseases caused by viral infection are affecting all major crops. being an obligate intracellular organisms, chemical control of these pathogens is so far not applied in the field except to control the insect vectors of the viruses. understanding of molecular responses of plant immunity is therefore economically important, guiding the enforcement of crop resistance. to disentangle complex regulatory mechanisms of the plant immune responses, understanding system as a whole is a must. howeve ... | 2017 | 28684682 |