| does rigidity in structure of muscarinic agonists and antagonists reflect drug specificity? | the present work is an attempt to elucidate: (1) whether highly rigid structural analogs of acetylcholine are still capable of activating the muscarinic receptor; (2) whether such analogs, be they agonists or antagonists, discriminate among the various ach-mediated functions, thereby providing a tool for the study of a possible receptor heterogeneity; (3) whether structural rigidity is a significant factor in the kinetics of drug-receptor interaction. to this end, we investigated some properties ... | 1980 | 7231438 |
| intracellular compartmentation and secretion of carp proinsulin synthesized in xenopus oocytes. | carp proinsulin and rabbit globin, synthesized in xenopus oocytes in response to simultaneously injected mrnas, are found in membrane vesicles and in the cytosol respectively. after 24 h of incubation labeled proinsulin, but not globin, is found also in the medium, indicating specific secretion from the oocytes. no insulin could be found either within or outside the oocytes. the results provide further evidence that the mrna for a given protein contains the information for its intracellular dest ... | 1981 | 7238527 |
| bothriocephalosis in the common carp in the eastern transvaal. | the first outbreak of bothriocephalosis in common carp in south africa is recorded. this condition was caused by bothriocephalus acheilognathi, a pseudophyllid tapeworm not previously identified in this country. methods to limit its spread are suggested. | 1980 | 7241498 |
| phosphoproteins of spring viremia of carp virus. | | 1981 | 7245617 |
| [svcv infection of carp (author's transl)]. | this word was carried out to confirm several pathogenical points which had been previously outlined during svcv infection trials of carp performed in autumn at + 11 degrees c, when the temperature was naturally decreased. here, 50 g carp which had undergone the wintering, were infected in spring either via the water-route (2 h in 5 x 10(3) pfu/ml water) or intraperitoneally (5 x 10(3) pfu/fish) when the natural elevation of the water temperature had reached + 11 degrees c. then the fish appeared ... | 1980 | 7259029 |
| trisomy 6qter. | a previously reported patient with trisomy for the distal part of 6q was shown by r-banding to be trisomic for 6q26qter, due to a t(6;22)(q26;p12) mat. altogether nine patients with 6qter trisomy have been reported. the main features of the 6qter trisomy syndrome are: severe mental and growth retardation; acrocephaly and brachycephaly; a carp-shaped mouth; micrognathia; a very short neck with unusual anterior webbing; joint contractures; the absence of severe inner organ malformations; and survi ... | 1981 | 7273465 |
| stimulation of transcription by s-adenosyl-l-homocysteine and virion-encapsidated methyl donor in spring viraemia of carp virus. | s-adenosyl-l-methionine (sam), a methyl donor, and its analogue s-adenyl-l-homocysteine (sah), an inhibitor of methylation, stimulate the activity of spring viraemia of carp virus (svcv) virion transcriptase. the stimulation observed for svcv is analogous to that observed previously (furuichi, 1974, 1978) for a totally unrelated virus, cytoplasmic polyhedrosis virus (cpv). in the absence of exogenous sam, rna with 5'-methylated termini (presumptive gpppampap) was produced, indicating that svcv h ... | 1981 | 7276915 |
| aporphines. 34. (-)-2,10,11-trihydroxy-n-n-propylnoraporphine, a novel dopaminergic aporphine alkaloid with anticonvulsant activity. | (-)-2,10,11-trihydroxy-n-n-propylnoraporphine (tnpa,2c) has been synthesized from thebaine (3a), via northebaine (3b), normorphothebaine (2a), and alkylation to the n-propyl derivative 2b. o-demethylation gave the desired product 2c. compound 2c showed activity comparable to its 10,11-dihyroxy counterpart (npa, 1b) on the stimulation of dopamine-sensitive adenylate cyclase in carp retinal homogenates. the evaluation of 2c on audiogenic seizures in mice, in the protection against paroxysimal eeg ... | 1981 | 7277402 |
| [effect of lutropins on oocyte maturation and ovulation in various carp-like fishes in vitro and in vivo]. | the study of biological effect of lh and iso-lh isolated from the hypophyses of higher vertebrates on two zoons of carp-like lower vertebrates, has shown that both preparations injected in vivo induce maturation of female loaches, while in the carp, iso-lh stimulates fish maturation and lh single and/or repeated doses (2-3 injections) do not provoke female maturation. both preparations in vitro stimulate the maturation process and the loach oocyte ovulation, but the character of this effect is d ... | 1981 | 7291144 |
| isolation and characterization of gonadotropin isohormones from the pituitary gland of pike eel (muraenesox cinereus). | pike eel gonadotropins were isolated from pituitary glands by 40% alcohol-6% ammonium acetate, ph 5.1 extraction and were purified by deae-cellulose chromatography and electrophoresis into four electrophoretically homogeneous forms. these four isohormones were biologically identified as gonadotropins by the stimulation of 32 p-uptake in 1 day-old chick testes, by the induction of ovulation in catfish, and by the in vitro production of testosterone from isolated rat leydig cells and of androgen f ... | 1981 | 7309369 |
| ionic mechanisms of two types of on-center bipolar cells in the carp retina. ii. the responses to annular illumination. | on-center bipolar cells in the dark-adapted carp retina were divided into four types (a, b, c, and d) on the basis of response wave forms, spectral response properties, and electrical membrane properties. type a and b cells responded to a spot of light with a transient depolarization followed by a plateau, whereas the response of type c and d cells were approximately rectangular in shape. the center and surround responses of type a cells had maximum spectral response of approximately 525 nm in t ... | 1981 | 7310376 |
| [etiology of erythrodermatitis of the carp]. | | 1981 | 7324642 |
| separating monosomy-21 from the "arthrogryposis basket". | an infant with monosomy-21 lived for 20 minutes. the clinical and radiologic findings suggested arthrogryposis, infants with monosomy-21 have severe intrauterine growth retardation, failure to thrive, arthrogryposis-like findings with restriction of joint mobility, often with joint dislocations, and flexion deformities and malposition of the fingers and toes. features which help to distinguish it from arthrogryposis include a rather specific craniofacial configuration with a broad base of the no ... | 1981 | 7328100 |
| a bacterial disease of perch (perca fluviatilis l.) in an alpine lake: isolation and preliminary study of the causative organism. | an epizootic among perch (perca fluviatilis l.) occurred during the summer and fall of 1979 in an alpine lake (lake annecy, haute-savoie) in france. hemorrhagic and ulcerative clinical signs were associated with the height of the mortality. a gram-negative, non-motile, slow-growing bacterium was isolated from skin lesions is diseased fish. aeromonas hydrophila often was present. since the nonmotile bacterium was suspected to be the etiological agent, its characteristics and pathogenicity were de ... | 1981 | 7338972 |
| [histopathological changes and localization of tapeworm khawia sinensis in the intestine of carps (cyprinus carpio l.)]. | | 1981 | 7345743 |
| [electrophoretic examination of the serum of carps (cyprinus carpio l.) infected with the tapeworm caryophyllaeus sp]. | | 1981 | 7345745 |
| [aspartate and alanine aminotransferase activity in the alimentary tract of carps (cyprinus carpio l.) infected with tapeworms bothriocephalus gowkongensis yeh, 1955 or khawia sinensis hsü, 1935]. | | 1981 | 7345746 |
| influence of nutritional status on the daily patterns of nitrogen excretion in the carp (cyprinus carpio l.) and the rainbow trout (salmo gairdneri r.). | nitrogen (ammonia and/or urea) excretion in carp and rainbow trout kept under different feeding regimes was continuously monitored over 24-hr cycles. the daily nitrogen excretion patterns resulting from these feeding regimes were studied over several days after a change from one made to another. constant levels of endogenous nitrogen excretion were reached about one week after the start of a fasting period; almost one week was needed for the daily nitrogen excretion pattern to stabilize after th ... | 1980 | 7349509 |
| interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate. | calmodulin has been purified to homogeneity using an improved procedure that allows rapid processing of several kilograms of bovine brain. a calcium-dependent change in the electrophoretic mobility of calmodulin in the presence of sodium dodecyl sulfate (sds) has been observed. freshly prepared calmodulin or lyophilized calmodulin, stored at --80 degrees c for 1--7 months, migrates as a single band with an apparent molecular weight of 21 000 when the sample, gel and running buffer are made 0.1 m ... | 1980 | 7397213 |
| size related toxicity of copper & mercury to lebistes reticulatus (peter), labeo rohita (ham.) & cyprinus carpio linn. | | 1980 | 7399616 |
| influence of quaternary structure on the state of the haem in carp and human methaemoglobins studied by resonance raman scattering. | the resonance raman spectra of carp and human methaemoglobin (methb) derivatives in the t and r quaternary structures were measured in the frequency regions of 1200-1700 cm-1 and 100-600 cm-1. conversion of carp and human fluoro methb's to the t structure was accompanied by a frequency shift of the raman line at 348 cm-1 to lower frequency by 5 cm-1, but all other lines were unaffected by the r-t transition. as the 348 cm-1 line was assigned to a porphyrin mode (v8) involving a bending motion of ... | 1980 | 7407241 |
| binding of non-mammalian immunoglobulins to staphylococcal protein a. | immunoglobulins m of the chicken (about 3% of serum igm), clawed toad (12%) and carp (13%) bind to the protein a-sepharose. a monomeric immunoglobulin with four domain heavy chains from the chicken serum is also bound (30% of bound immunoglobulins). the protein a-sepharose can be utilized for isolation of igm of the clawed toad and carp directly from the serum. | 1980 | 7409269 |
| investigations on the origin of two radiation-induced compounds in irradiated meat. | after irradiation with 0.5 mrad or higher doses, a radiation-induced ninhydrin-positive compounds was found in the muscle tissue of warm-blooded animals. in carp muscle another compound called x was formed. the origin of these two compounds was investigated. irradiation of various amino acids and related compounds showed that substance y is formed from the dipeptides carnosine and anserine, which are present in muscle tissue of warm-blooded animals unusually high concentrations. in carp muscle c ... | 1980 | 7415531 |
| [comparative studies of the specificity of idiotypic guinea pig and carp antisera]. | the specificity of guinea-pig- and carp-anti-idiotypic antisera were compared by using the radioimmunoassay and the passive hemagglutination inhibition test. guinea-pigs and carps were immunized with the iga/k mouse myeloma protein s117 which has binding activity to the hapten n-acetylglucosamine. the resulting antisera were idiotypically specified by absorption and the activity was determined by the binding of the radiolabeled idiotype s117. the binding curves of guinea pig and carp anti-idioty ... | 1980 | 7424349 |
| carp hemoglobin. i. precise oxygen equilibrium and analysis according to the models of adair and of monod, wyman, and changeux. | precise oxygen equilibria for carp hemoglobin had been measured from about 1% to 99% saturation at 15 degrees c. the determinations were made in 2,2-bis(hydroxymethyl)-2,2',2"-nitrilotriethanol buffer and in phosphate buffer in the presence and absence of p6-inositol (ihp). at the highest cooperativity, carp hemoglobin has a maximum hill coefficient, n, of 2.3 and free energy of interaction, delta gi, of 2100 cal/site; addition of ihp decreases these values to 1.6 and 1640 cal/site, respectively ... | 1980 | 7430103 |
| [hemoglobins, xxxv: the sequence of the beta a- und beta b-chains of the hemoglobins of the carp (cyprinus carpio l.)]. | the primary structure of the beta a- and beta b-chains from both main components of the tetrameric hemoglobins from carp is given. the sequences were found by automatic analysis in the sequenator using a hydrophilic and hydrophobic program with hydrophilic isothiocyanate. the beta-chains a and b differ in positions beta 13, beta 41, beta 122 and beta 143. in comparison to human beta-chains carp beta a- and beta b-chains show 66 and 67 residues, respectively (45%). furthermore, we found one inser ... | 1980 | 7450680 |
| comparison of the pineal complex, retina and cerebrospinal fluid contacting neurons by immunocytochemical antirhodopsin reaction. | the presence of rhodopsin was investigated by an indirect immunocytochemical method in the pineal complex of various vertebrates (carassius auratus, cyprinus carpio, hypophthalamichthys molitrix, lucioperca lucioperca, triturus vulgaris, bombina bombina, rana esculenta, pseudemys scripta elegans, lacerta agilis et viridis, white leghorn chickens, rat), in the retina of lebistes reticulatus, lucioperca lucioperca, rana esculenta, lacerta agilis, pseudemys scripta elegans, the chicken and the rat, ... | 1980 | 7456628 |
| an immunocytochemistry study comparing the occurrence of neuroactive substances in the nervous system of cercariae and metacercariae of the eye fluke diplostomum spathaceum. | the nervous system of two larval stages (cercariae, metacercariae) of eye fluke diplostomum spathaceum was investigated immunocytochemically by the application of antisera to the amino acid glutamate and to neuropeptides isolated from invertebrates (mollusca) and from vertebrates to whole-mount preparations. in cercariae, positive immunoreactivity (ir) was observed with antisera raised against catch-relaxing peptide (carp), fmrfamide, alpha-caudodorsal cell peptide (alpha-cdcp), substance p, vas ... | 1995 | 7479646 |
| [15n-labeling of fishes using 15n isotopes in aquarium water and the effect of a different protein nutrient on the 15n elimination after the labeling period]. | in a preexperiment of 12 days fishes (cyprinus carpio l.) were labelled with 15n by means of 15nh4cl and 15n-urea resp. in the aquarium water and by feeding a protein free diet. 15nh4cl yielded a higher atom-% 15n excess (15n') in the tissues of fishes. in the main experiment 75 fishes (cyprinus carpio l.) were 15n-labelled with 100 mg 15n'/l water from 15nh4cl (95 atom-% 15n') in a protein free preperiod of 12 days. in the following main period the fishes received different protein sources in t ... | 1993 | 7487475 |
| synergism of gh and igf-i in stimulation of sulphate uptake by teleostean branchial cartilage in vitro. | the combined effects of teleostean gh and human igf-i in the regulation of teleost branchial cartilage growth were examined. ceratobranchial cartilages were dissected from the common carp (cyprinus carpio) and maintained in a defined culture medium supplemented with recombinant striped bass gh (sbgh), human ifg-i (higf-i) or both, and the uptake of [35s]sulphate by cartilage explants was measured. sbgh alone in the culture medium did not exhibit a significant stimulatory effect on the uptake of ... | 1995 | 7490539 |
| effect of exposure to malachite green solution on common carp fry with dactylogyrus vastator (monogenea) infection. | in five experiments, common carp (cyprinus carpio) fry with dactylogyrus vastator (monogenea) infection of different severity, as well as parasite-free fry, were placed into aquaria containing malachite green solution of 0.5-10 mg/l concentration. most of the fish died within 6 hours. the correlation between the time of death and helminth infection was studied. the experiments demonstrated that in malachite green solution the carp with the heaviest helminth infection died first while the less se ... | 1995 | 7491866 |
| structural and biochemical remodelling in catecholamine-induced cardiomyopathy: comparative and ontogenetic aspects. | excessive release or administration of beta-mimetic catecholamines may induce cardiomegaly, necrotic lesions and accumulation of connective tissue in the heart of adult homoiotherms. it was examined here whether similar changes can also be observed at different stages of evolution of the cardiovascular system, i.e. in poikilotherms and in homoiotherms during embryonic life. sensitivity of the poikilothermic hearts (carp, frog, turtle) to isoproterenol (ipro) was significantly lower than in the h ... | 1995 | 7494559 |
| central nervous system abnormalities in chromosome deletion at 11q23. | two japanese pediatric patients with terminal deletion of the long arm of chromosome 11 are described. both had the morphological abnormalities of the 11q deletion syndrome, such as prominent epicanthal folds, broad flat nasal bridge with short, upturned nose, short philtrum with carp-shaped mouth, cardiac anomalies and nonprogressive moderate psychomotor developmental delay. patient 1 is the first case to be reported with 11q deletion with serial magnetic resonance (mr) examinations of cerebral ... | 1994 | 7523004 |
| recognition of surface antigens on spermatozoa of the common carp (cyprinus carpio l., teleostei) using monoclonal antibodies and scanning electron microscopy. | the distribution of antigenic determinants, recognized by seven anti-carp spermatozoa monoclonal antibodies (mabs) and two anti-carp spermatogonia mabs were studied using fresh, unfixed carp spermatozoa. the location of the antigenic determinants was analysed in light- and in scanning electron microscopy, in the latter with the backscattered imaging mode. with all seven anti-carp spermatozoa mabs a similar regular distribution of the immuno-gold labeling was present on heads, midpieces and tails ... | 1995 | 7536015 |
| insulin and igf-i binding and tyrosine kinase activity in fish heart. | a study is presented of the binding of insulin and igf-i to their respective receptors in the heart muscle of carp (cyprinus carpio), coho salmon (oncorhynchus kisutch), brown trout (salmo trutta fario) and sprague-dawley rats. receptor preparations were partially purified by wheat germ agglutinin affinity chromatography. specific binding of insulin/100 mg cardiac muscle was much lower in fish (from 5.6 to 9.2%) than in rat (52.0 +/- 5.0%). in both carp and trout, insulin binding to the cardiac ... | 1995 | 7561619 |
| chimeric l-type ca2+ channels expressed in xenopus laevis oocytes reveal role of repeats iii and iv in activation gating. | 1. chimeric alpha 1 subunits consisting of repeat i and ii from the rabbit cardiac (alpha 1c-a) and repeat iii and iv from the carp skeletal muscle ca2+ channel (alpha 1s) were constructed and expressed in xenopus laevis oocytes without co-expressing other channel subunits. ba2+-current kinetics of five chimeric channel constructs were studied in xenopus oocytes using the two-microelectrode technique. 2. exchange of repeats iii and iv of alpha 1c-a with sequences of alpha 1s results in a signifi ... | 1995 | 7562629 |
| purification properties and specificity of cathepsin d from cyprinus carpio. | cathepsin d was purified 750-fold from a homogenate of cyprinus carpio muscles. the purified enzyme has a molecular weight of 36,000, is inhibited by pepstatin and is active between ph 2.7 and 3.7 when tested on hemoglobin as the substrate. it consists of two isoenzymes with pis of 5.65 and 6.1, respectively. the mode of cleavage of the beta chain of oxidized insulin was determined by analysis of the n-terminal amino acids of the cleaved peptides. the major points of cleavage of the beta chain o ... | 1995 | 7584842 |
| evidence for a direct action of gh on haemopoietic cells of a marine fish, the gilthead sea bream (sparus aurata). | receptors for gh were characterized in the head kidney of gilthead sea bream (sparus aurata), using radioiodinated and biotinylated ligands. the specific binding of radiolabelled recombinant gilthead sea bream gh (rsbgh) to head kidney membrane preparations was dependent on membrane concentration. salmon prolactin, salmon gonadotrophin and carp gonadotrophin did not compete for 125i-labelled rsbgh-binding sites. unlabelled rsbgh competitively displaced 125i-labelled rsbgh bound to head kidney me ... | 1995 | 7595141 |
| parasites of south african freshwater fish. vii. nematodes of some scaled fishes from the hartbeespoort dam, transvaal. | the nematode parasites of 16 large-scaled yellowfish, barbus marequensis, six silverfish, barbus mattozi, six small-scaled yellowfish, barbus polylepis, 52 canary kurper, chetia flaviventris, 11 carp, cyprinus carpio, 45 mozambique bream, oreochromis mossambicus and a single-banded bream, tilapia sparrmani, caught in the hartbeespoort dam, transvaal, were collected, identified and counted. contracaecum spp. larvae were recovered from one o. mossambicus, 40 chetia flaviventris, three cyprinus car ... | 1994 | 7596571 |
| carp macrophages and neutrophilic granulocytes secrete an interleukin-1-like factor. | carp, cyprinus carpio l, macrophages and neutrophilic granulocytes obtained from pronephros were cultured. supernatant was harvested after 48 h and tested for interleukin-1 (il-1) bioactivity. a concentration-dependent stimulation of proliferation was found of carp ig- lymphocytes as well as of the murine il-1 dependent d10 (n4) m cell line. a 4 h treatment of cells with phorbol myristate acetate prior to culture gave a two- to fourfold enhancement of the bioactivity in the supernatant. antibodi ... | 1995 | 7615138 |
| pyrimidine regulation of the escherichia coli and salmonella typhimurium carab operons: carp and integration host factor (ihf) modulate the methylation status of a gatc site present in the control region. | by measuring the protection against dam methylase modification of a gatc sequence located 106 bp upstream of the startpoint of promoter p1 in the control region of the carab operon (encoding carbamoylphosphate synthetase) we have obtained evidence for a direct correlation between the degree of in vivo occupancy of a specific regulatory target site and the repressibility of the p1 promoter by pyrimidine residues. a high uridine nucleotide pool as well as binding of the carp (alias xerb/pepa) gene ... | 1995 | 7616563 |
| carp, involved in pyrimidine regulation of the escherichia coli carbamoylphosphate synthetase operon encodes a sequence-specific dna-binding protein identical to xerb and pepa, also required for resolution of colei multimers. | the carp gene involved in pyrimidine-specific regulation of the upstream p1 promoter of the escherichia coli carab operon has been cloned in vivo on a mini-mu replicon, sequenced and shown to be identical to the xerb (pepa) gene encoding aminopeptidase a, a protein also involved in the xer-mediated site-specific recombination at colei cer. the trans-dominant allele carp6 was cloned as well and shown to bear a single g-->a transition that converts the tgg codon (trp473) into a tag amber stop codo ... | 1995 | 7616564 |
| immunolabelling of fish host molecules on the tegumental surface of ligula intestinalis (cestoda: pseudophyllidea). | immunoblotting, sds-page and western blotting procedures were used to demonstrate cross-reactivity of a polyclonal anti-carp igm antibody with components of roach serum. the polyclonal antibody labelled 2 major bands in both immune and normal roach sera corresponding to molecular masses of approximately 90 and 65 kda. one of these bands (65 kda) was considered to be heavy chain of fish immunoglobulin whilst the identity of the other remains uncertain. this cross-reaction has been exploited in im ... | 1995 | 7622332 |
| characterization of gamma-crystallin from a catfish: structural characterization of one major isoform with high methionine by cdna sequencing. | gamma-crystallin is the major and most abundant lens protein present in the eye lens of most teleostean fishes. to facilitate structural characterization of gamma-crystallins isolated from the lens of the catfishes (clarias fuscus), a cdna mixture was synthesized from the poly(a)+mrna isolated from fresh eye lenses, and amplification by polymerase chain reaction (pcr) was adopted to obtain cdnas encoding various gamma-crystallins. plasmids of transformed e. coli strain jm109 containing amplified ... | 1995 | 7627123 |
| aii amacrine cell population in the rabbit retina: identification by parvalbumin immunoreactivity. | parvalbumin (pv) is a calcium-binding protein localized to selected neurons in the nervous system, including the retina. this investigation evaluated the distribution of pv immunoreactivity in the rabbit retina using immunohistochemistry with a monoclonal antibody directed to carp pv. in the inner nuclear layer (inl), pv immunoreactivity was present in horizontal and amacrine cells. in the ganglion cell layer, pv immunostaining was confined to somata that are likely to be both displaced amacrine ... | 1995 | 7629307 |
| a human factor that recognizes dna substituted with 2-chloroadenine, an antileukemic purine analog. | 2-chloro-2'-deoxyadenosine (cladribine), an analog of deoxyadenosine, is an important new drug for the treatment of hairy cell leukemia and other forms of adult and pediatric leukemia. by a gel-shift binding assay, we identified an activity in hela nuclear extracts that recognizes and binds to oligonucleotides substituted with 2-chloroadenine (clade). the activity was specific for clade residues because control oligomers did not readily compete out the complex. the binding factor was a monomeric ... | 1995 | 7646763 |
| the use of recombinant gilthead sea bream (sparus aurata) growth hormone for radioiodination and standard preparation in radioimmunoassay. | a gilthead sea bream growth hormone (sbgh) obtained by cloning and expression of sbgh cdna was used to develop a sensitive and specific radioimmunoassay (ria). iodination of recombinant sbgh (rsbgh) was performed by the classical chloramine-t method. specific antiserum, raised in rabbits, was added in a final dilution of 1/36,000. the minimum detectable dose was 30 pg, and the midrange of the assay (ed50) was 275 pg. intra- and inter-assay coefficients of variation (cv) were 3.3 and 5.8% at ed50 ... | 1995 | 7669108 |
| use of 5'-bromodeoxyuridine immunohistochemistry to examine proliferative activity of fish tissues. | the suitability of bromodeoxyuridine (brdu) immunohistochemistry for the study of myosatellite cell proliferation in three subadult carp (cyprinus carpio) stages (11, 15, 17 cm) was examined. they were injected intraperitoneally with brdu and fixed one hour late. after fixation and dehydration, white myotomal muscle and small intestine samples were embedded in histowax. cross sections mounted on glass slides, were incubated with monoclonal antibrdu antibody (1:100) after hcl denaturation. after ... | 1993 | 7688604 |
| major glycoproteins in carp cns myelin: homology to p0 protein with hnk-1/l2 carbohydrate epitope. | we studied the myelin protein profiles of carp from a phylogenetic point of view. the carp central nerve myelin contained two reactive bands, 28 and 25 kda, demonstrated with anti-bovine p0 antibody. their molecular weights are slightly different from those of two positive bands found in carp peripheral myelin. the n-terminal amino acid sequences of these four positive bands were identical to one another and showed high homology with those of mammalian p0 protein, suggesting that carp central my ... | 1993 | 7693109 |
| m-band structure, m-bridge interactions and contraction speed in vertebrate cardiac muscles. | cardiac muscle m-band structures in several mammals (guinea pig, rabbit, rat and cow) and also from three teleosts (plaice, carp and roach), have been studied using electron microscopy and image processing. axial structure seen in negatively stained isolated myofibrils or negatively stained cryo-sections shows the presence of five strong m-bridge lines (m6, m4, m1, m4' and m6') except in the case of the teleost m-bands in which the central m-line (m1) is absent, giving a four-line m-band. the m4 ... | 1994 | 7706420 |
| the distribution and host range of species of the blood fluke sanguinicola in british freshwater fish. | the distribution of host range of sanguinicola armata, s. inermis and s. volgensis in british freshwater fish are described. s. inermis has been recorded in carp cyprinus carpio, crucian carp carassius carassius and tench tinca tinca in southern, central and eastern england and in koi carp imported from japan. s. armata has been detected in pike esox lucius and tench and s. volgensis has been found in chub leuciscus cephalus, dace leuciscus leuciscus, perch perca fluviatilis, roach rutilus rutil ... | 1994 | 7706678 |
| [evaluation of 4 vermifuges against bothriocephalus acheilognathi in carp]. | with the aim to evaluate the efficacy of four vermicides, praziquantel, mebendazole, nitroscanate and triclorfon in the control of bothriocephalus acheilognathi in carps, one hundred and sixty, two year-old carps (ctenopharyngodon idellus) with an average weight of 120 g and a mean parasitic burden of 115.5 parasites/fish were used. five groups of 30 fish each were randomly formed. each group was then subdivided in three groups, each of 10 individuals/pond. the three subgroups of group a receive ... | 1994 | 7709095 |
| ecopathology in aquaculture: risk factors in infectious disease outbreak. | this paper describes a study of the risk factors associated with disease outbreaks in fish species of fish farms and rivers of north-east spain. we focused our work on the isolation of fish pathogens (bacteria, virus), the water quality (physicochemical and microbiological quality) and management characteristics. we have observed 2 important viral diseases, infectious pancreatic necrosis and spring viraemia of carp, and 2 important bacterial ones, furunculosis (aeromonas salmonicida) and bacteri ... | 1995 | 7711777 |
| hypothalamic and thyroidal regulation of growth hormone in tilapia. | a radioimmunoassay (ria) for recombinant tilapia growth hormone (gh) was established and validated. the ability of various hypothalamic factors to regulate gh secretion in the tilapia hybrid (oreochromis niloticus x oreochromis aureus) was studied. somatostatin1-14 (srif1-14; 10-100 micrograms/kg) was found to reduce circulating gh levels in a dose-dependent manner. srif1-14 (0.1-1000 nm) inhibited gh release from perifused pituitary fragments (ed50 0.83 nm). human growth hormone-releasing hormo ... | 1995 | 7713377 |
| experimental streptococcal meningo-encephalitis in cultured fish. | in 1984 a disease of fish appeared in israel which spread rapidly in cultured fishponds. the disease affected tilapia (oreochromis aura x oreochromis nilotica hybrids) and trout (oncorhynchus mykiss). common carp (cyprinus carpus), although reared in community with tilapia were not susceptible to the disease. various species of ornamental cyprinids and cichlids were also affected. morbidity was high and mortality ranged between 50% (in trout) and 30% (in tilapia). clinical and pathological findi ... | 1995 | 7716882 |
| cyclic amp-reactive proteins in human saliva. | this study was conducted to compare cyclic amp-reactive proteins (carp), the secretory form of regulatory (r) subunits of cyclic amp-dependent protein kinase (pka), and cyclic nucleotide phosphodiesterase (pde) activity in human whole saliva with that of parotid fluid. additionally, experiments were done to determine whether secretory carp is altered by environmental stimuli. earlier work showed that r subunits are present in parotid fluid and in salivary glands of rats. no previous information ... | 1994 | 7741656 |
| interstitial deletion of 8q21-->22 associated with minor anomalies, congenital heart defect, and dandy-walker variant. | we describe an infant with a deletion of 8q21-->22 who had distinct clinical manifestations including minor facial anomalies, a congenital heart defect, a dandy-walker variant, and mild to moderate developmental delay. her facial characteristics included small, wide-spaced eyes, asymmetric bilateral epicanthal folds, a broad nasal bridge, a "carp-shaped" mouth, micrognathia, and prominent, apparently low-set ears. three other reports describe children with larger proximal deletions of 8q that in ... | 1995 | 7747796 |
| on the role of the escherichia coli integration host factor (ihf) in repression at a distance of the pyrimidine specific promoter p1 of the carab operon. | binding of integration host factor to its target site, centered around nucleotide -305 upstream of the transcription startpoint, exerts antagonistic effects on the expression of p1, the upstream pyrimidine specific promoter of the e coli and s typhimurium carab operons. ihf stimulates p1 promoter activity in minimal medium, but also increases the repressibility of this promoter by pyrimidines. we present evidence strongly suggesting that ihf exerts these effects by modulating the binding of anot ... | 1994 | 7748925 |
| development of position-independent expression vectors and their transfer into transgenic fish. | many genes have been transferred into fish for scientific and aquacultural purposes. we have been developing expression vectors containing regulatory sequences from the carp beta-actin gene enhancer/promoter for expression of genes or cdnas in transgenic fish. expression from these vectors varies over a 20-fold range in zebrafish, beginning within 12 hours of fertilization and continuing for at least two weeks. expression can be found in nearly all tissues. the vectors have the following charact ... | 1995 | 7749466 |
| comparative inhibition patterns of adenylate kinases from mammals, bird, fish and microorganisms. | the s8 inhibitions of aks from six different sources were studied in mammals, birds, fish, and a microorganism. all aks tested were inhibited by s8. except for carp, all inhibited aks from those tested were reactivated by dtt. inhibitions of aks by other hydrophobic inhibitors, nem, butanol and ethanol were also studied. the inhibitions by s8 suggest that the hydrophobic pockets in the aks cover a wide phylogenetic range. all inhibitions by s8 are reactivated by dtt. unlike the inhibitions by s8 ... | 1994 | 7749617 |
| production of a biologically active recombinant teleostean growth hormone in e. coli cells. | we have isolated and characterized several recombinant lambda phage clones carrying growth hormone (gh) cdna of striped bass (morone saxatilis). nucleotide sequence and the predicted amino acid sequence of sbgh was determined from a recombinant clone carrying the longest cdna insert. the sbgh cdna encodes a pre-hormone of 204 amino acid residues. comparison of the predicted amino acid sequence of sbgh with those of other vertebrates revealed different degrees of sequence identity: approximately ... | 1995 | 7758842 |
| effect of arterially infused catecholamines and insulin on plasma glucose and free fatty acids in carp. | common carp (cyprinus carpio l.), kept at 20 degrees c, were fitted with an indwelling pe-50 cannula in the dorsal aorta. hormones dissolved in ringer saline were arterially infused at a rate of 1 microgram.kg-1.min-1 for epinephrine (epi), 2 micrograms.kg-1.min-1 for norepinephrine (ne), and 1.33 micrograms.kg-1.min-1 for insulin. infusion of bovine insulin in carp resulted in a long lasting (24 h) decrease of plasma free fatty acids (ffa; -0.41 +/- 0.06 mm) and glucose levels (-3.14 +/- 0.25 m ... | 1995 | 7771575 |
| abundant insulin-like growth factor-1 (igf-1) receptor binding in fish skeletal muscle. | insulin and insulin-like growth factor-1 (igf-1) receptors were quantified in glycoprotein fractions prepared by wheat germ agglutinin-agarose affinity chromatography from skeletal muscle of several species of salmonid fish and carp. insulin binding in fish skeletal muscle was lower than insulin binding found in rat. igf-1-specific binding was two- to sixfold higher than insulin binding (16.0 +/- 3.0 versus 5.8 +/- 0.75%/50 micrograms glycoprotein in brown trout, 15.5 +/- 0.9 versus 2.2 +/- 0.5% ... | 1995 | 7781961 |
| effects of prolactin and fish pituitary extract on plasma calcium levels in common carp, cyprinus carpio. | the effects of prolactin (prl) from both mammalian and piscine sources on plasma calcium levels in common carp, cyprinus carpio, were investigated. injections of ovine prolactin (oprl) or homologous fish pituitary extract or partially purified prolactin of murrel, channa punctatus pituitary (mprl), caused significant increases in total and ultrafiltrable plasma calcium. larger hypercalcemic responses were observed in fish kept in high-calcium fresh water and normal tap water than in fish in low- ... | 1995 | 7789747 |
| survey of the paratenic hosts of anguillicola crassus in lake velence, hungary. | anguillicola crassus infection of paratenic hosts was investigated in lake velence, hungary, in october 1992. a total of 155 specimens of 8 species (gymnocephalus cernuus, lepomis gibbosus, stizostedion lucioperca, abramis brama, alburnus alburnus, cyprinus carpio, pseudorasbora parva and rutilus rutilus) were examined: all species proved to be infected. there were large differences in the prevalence of infection among fishes, but the prevalence was generally higher in perciform fishes and it wa ... | 1994 | 7810405 |
| the occurrence of neurons with strongly negatively charged surface coats in mammalian, avian, reptilian, amphibian and piscine brains. | neurons with strongly negatively charged surface coats were recognized in mammalian, avian, reptilian, amphibian and piscine brains. many large-sized neurons had strongly negatively charged surface coats in the visual cortex and brain stem of the cow, cat, guinea pig, mouse, quail and parakeet. such neurons were also seen in the brain stem of the lower vertebrates such as the house lizard, japanese terrapin, bullfrog, newt, carp and sweetfish. | 1994 | 7817774 |
| [ambiphrya ameiuri (ciliophora: peritricha): its ultrastructure and distribution on the body of carp fry]. | the mass infection of larvae and youngsters of carp fishes with the peritrichoid ambiphrya ameiuri has been recorded in natural condition in the rybinsk water reservoir. the uneven localisation of ambiphyrans on the body surface has been observed on the hosts blicca bjorkna and abramis brama. most high density of parasites was on the breast, back and tail fins. the morphological variability of ambiphyrans in dependence upon their localisation on the fish body has been observed. details being sig ... | 1994 | 7824286 |
| immunocytochemical localization of granulin-1 to mononuclear phagocytic cells of the teleost fish cyprinus carpio and carassius auratus. | a new class of low-molecular-weight cysteine-rich regulatory growth factors, designated granulins, has been isolated from hematopoietic tissues of a teleost fish (cyprinus carpio) and structurally characterized. granulin-1, the predominant form found in carp spleen, was used to raise polyclonal antibodies in rabbits and to establish a radioimmunoassay. this permitted preliminary tissue distribution studies of granulin-1 to be undertaken in carp (cyprinus carpio) and goldfish (carassius auratus). ... | 1995 | 7829976 |
| development of a homologous radioimmunoassay for coho salmon insulin-like growth factor-i. | a specific homologous radioimmunoassay (ria) for measurement of insulin-like growth factor-i (igf-i) in plasma of salmonid and a few non-salmonid fish species was developed using recombinant coho salmon igf-i (rsigf-i) as tracer and standard, and antiserum against this peptide raised in rabbits. the minimum detection level of igf-i was 1.5 ng/ml and linearity was obtained in a range from 1.5 to 23 ng/ml. no cross-reaction was detected in the salmon igf-i ria with mammalian growth factors, salmon ... | 1994 | 7843563 |
| up-regulation of insulin binding in fish skeletal muscle by high insulin levels. | the effects of insulin titres on regulation of receptor binding were studied in several fish species. insulin receptors were semi-purified by affinity chromatography (wga-agarose) from skeletal muscle of carp, brown trout and rainbow trout that had been subjected to increases in insulinemia produced either by arginine injection, food administration, or adaptation to an experimental diet (extruded diet with high-digestibility carbohydrates). arginine injection provoked acute hyperinsulinemia in b ... | 1994 | 7846297 |
| two c-myc genes from a tetraploid fish, the common carp (cyprinus carpio). | two c-myc genes have been isolated from a tetraploid fish, the common carp (cyprinus carpio). the nucleotide (nt) sequences of the two genes and their flanking regions (cam1, 8675 bp and cam2, 5784 bp) have been determined. both genes contain two coding exons which are homologs of c-myc exons 2 and 3 in higher vertebrates. the nt sequence homologous to c-myc exon 1 of higher vertebrates has not been found. carp cam1 and cam2 presumably encode peptides of 394 and 401 amino acids (aa), respectivel ... | 1995 | 7875594 |
| the regulatory region and transcription factor required for the expression of rat and salmon pituitary hormone-encoding genes show cell-type and species specificity. | the promoter regions of the genes encoding the rat and chum salmon growth hormones (gh) and rat prolactin (prl) were combined with a reporter gene and introduced into gh- and/or prl-producing cells from rat. the rat gh and prl promoters (pgh and pprl, respectively) were most active in cells producing gh and prl, respectively. the activity of the salmon pgh was much less than that of the rat pgh in rat gh-producing cells. the regulatory region required for cell-type-specific gene expression of pi ... | 1995 | 7875599 |
| [the perceptive color space in the carp (carpio cyprinus l.)]. | discrimination of colours was studied using choice conditioning paradigm (one of a pair of colour stimuli) in carps. matrix of probabilities of instrumental reactions (catching a bead) was constructed by the results of eight experimental sessions with successive reinforcement of one of the eight colours. it was processed by factor analysis. the spherical structure of carp's perceptual colour space revealed by factor analysis was similar to that of monkeys and humans. four eigenvectors which cons ... | 1994 | 7879429 |
| response of ig-positive cells to goussia carpelli (protozoa: apicomplexa) infections in carp (cyprinus carpio l.). | the kinetics of ig-positive cell populations in carp tissues was followed during an infection with the gut dwelling coccidian goussia carpelli léger et stankovich, 1921. in cell suspensions of the anterior and posterior sections of the intestine, the proportion of ig-positive cells increased with the development of the coccidia and peaked during oocyst formation at day 15 post exposure. these results suggest a reaction of the local mucosal immune system. in cell suspensions of pronephros the pro ... | 1994 | 7883249 |
| temperature acclimation induces light meromyosin isoforms with different primary structures in carp fast skeletal muscle. | carp acclimated to 10 degrees c gave 69k, 66k, and 62kda light meromyosin (lmm) fragments in sds-page, while fish acclimated to 30 degrees c gave 74k, 69k, 66k, and 62kda fragments. the microsequence analysis revealed that the 69k and 66kda components from the 10 degrees c-acclimated carp contained an n-terminal amino acid sequence different from that of 62kda. the four fragments from the 30 degrees c-acclimated carp showed the same sequence as that of the 69k and 66kda components from the 10 de ... | 1995 | 7887920 |
| relationships among ryanodine receptor isoforms expressed in vertebrate skeletal muscles based on immunologic cross-reactivities. | to clarify the relationship among ryanodine receptor isoforms from skeletal muscles of various vertebrates (alpha- and beta-isoforms with smaller and larger mobilities on sds-polyacrylamide gel electrophoresis, respectively), we examined the cross-reactivities of those receptors from 13 animal species by western blot analysis, using specific polyclonal antibodies raised in rabbit against the bullfrog alpha- and beta-isoforms. anti-beta-isoform antibody showed positive reactions against skeletal ... | 1994 | 7896740 |
| cystine/glutamate antiporter expression in retinal müller glial cells: implications for dl-alpha-aminoadipate toxicity. | a cytotoxicity of glutamate or related amino acids (10 mm) mediated by a cystine/glutamate antiporter (system xc) has recently been demonstrated in n18 neuroblastoma-rat retina hybrid (n18re105) cells and c6 glioma cells. the antiporter usually transports glutamate outside and cystine inside, thereby maintaining cellular concentrations of glutathione. high concentrations of glutamate inhibit cystine uptake and lead to depletion of cellular levels of glutathione. among related amino acids, dl-alp ... | 1993 | 7906874 |
| spring viraemia of carp. | | 1994 | 7941273 |
| role of the atrial natriuretic peptide for adrenal regulation in the teleost fish cyprinus carpio. | the biological role of atrial natriuretic peptide (anp) for the adrenal tissue of the teleost fish cyprinus carpio, the carp, was investigated using in vitro autoradiography to study anp binding sites, immunohistochemistry to localize anp-like peptides, and in vitro perifusion to measure adrenal cortisol and catecholamine release. 125i-labeled rat anp-(99-126) [125i-ranp-(99-126)] binding was present in adrenal tissue exhibiting positive cooperativity with a half-maximal binding concentration (e ... | 1994 | 7943413 |
| monoclonal antibodies against goldfish (carassius auratus) immunoglobulin: application to the quantification of immunoglobulin and antibody-secreting cells by elispot and seric immunoglobulin and antibody levels by elisa in carp (cyprinus carpio). | monoclonal antibodies (mabs) raised against heavy and light chains of goldfish immunoglobulin (ig) were characterized by a western blot technique. a complete cross-reactivity was observed between carp and goldfish ig. these mabs were used for the quantification of carp ig and anti-yersinia ruckeri antibodies by elisa. an elispot assay was also developed in carp to quantify ig-secreting cells (isc) and antibody-secreting cells (asc). the number of asc was maximum on day 18 post-vaccination and de ... | 1994 | 7951348 |
| immunodetection of the ribosomal transcription factor ubf at the nucleolus organizer regions of fish cells. | a human autoimmune serum to nucleolus organizer regions (nors) has been used to localize these structures at the light microscopic level in carp and trout tissue culture cells. in interphase cells, the immunofluorescence pattern indicates that the nors autoantigens are contained exclusively within the nucleolus of carp epithelial (epc) and trout gonad (rtg) cells. this fluorescence is punctuate rather than uniform, and presumably represents transcriptional complexes of ribosomal dna. during mito ... | 1994 | 7954874 |
| dnase-i-like enzyme from the carp liver--inhibition by muscle and endogenous actin. | 1. dnase-i-like activity occurs in the carp (cyprinus carpio) liver cytosol (supernatant 105,000 g). 2. the enzyme resembles dnase i from bovine pancreas in respect to the molecular mass (approximately 31 kda), ph (7.4) and ion requirements (mg2+, ca2+) and the ability to degrade native as well as denatured dna. 3. as judged by comparison of dnase zymograms obtained after native- and sds-page, the enzyme occurs in the three molecular forms of similar molecular weight and different charges. 4. al ... | 1994 | 7988739 |
| cloning and characterization of the european seabass, dicentrarchus labrax, mitochondrial genome. | mitochondrial dna (mtdna) from the european seabass, dicentrarchus labrax, has been cloned and characterized. its gene organization was deduced by a comparison of the sequenced termini of different subclones obtained from european seabass mtdna to the completely-sequenced mtdnas from carp and freshwater loach. the difference in genome size between the european seabass mtdna (approximately 18 kb) and most of the other characterized fish mtdnas (approximately 16.5 kb) is accounted for by the displ ... | 1994 | 8001168 |
| properties of the 'neutral zone' explain polarity of retinal spreading depression. | retinal spreading depression was evoked using low cl- ringer's solution and the concomitant field potentials (spreading depression potential; sdp) were recorded. the polarity of the transretinally recorded sdps was not consistent among animal species. the sdps recorded from carp and frog were receptor side negative, while chick and cat induced receptor side positive sdps. according to the k+ hypothesis, the retinal sdp is generated by müller cells responding to an increase in the extracellular k ... | 1994 | 8008248 |
| studies on the toxic and immunosuppressive effects of cadmium on the common carp. | in the last couple of decades, research on cadmium extended from its toxicologic aspects to its role in environmental pollution. the toxicity of cadmium to different species of fish varies widely. the cadmium sensitivity of batches of common carp, the most important cultured fish species of hungary, was studied. lethality, histopathologic changes and effect of cadmium on the antibody response, lysozyme level and microbicidal capacity were monitored. wide variation was found in individual sensiti ... | 1993 | 8017244 |
| immunocytochemical study on biologically active neurosubstances in daughter sporocysts and cercariae of trichobilharzia ocellata and schistosoma mansoni. | immunocytochemical techniques applied to sections and whole-mount preparations of cercariae from two species of trematodes, trichobilharzia ocellata and schistosoma mansoni, revealed the occurrence of immunoreactivity (ir) to several neurosubstances in the nervous system (ns). immunostaining was localized in cerebral ganglia, in the main commissure, in anterior and posterior nerve trunks, as well as in a pair of nerve fibres running along the tail. in t. ocellata, immunoreactivity (ir) was obser ... | 1994 | 8022656 |
| insulin binding and receptor tyrosine kinase activity in skeletal muscle of carnivorous and omnivorous fish. | we characterized the insulin receptors in skeletal muscle from several fish species with different nutritional preferences: brown trout (salmo trutta fario), gilthead sea bream (sparus aurata), tilapia (tilapia mossambica), and carp (cyprinus carpio), semipurified by affinity chromatography (wheat germ agglutinin-agarose). total specific binding and number of receptors per unit weight of piscine white skeletal muscle were lower than those values found in mammalian skeletal muscle. the same param ... | 1994 | 8024051 |
| characterization of gamma-crystallins from a hybrid teleostean fish: multiplicity of isoforms as revealed by cdna sequence analysis. | gamma-crystallins were isolated and characterized from the eye lenses of a hybrid species belonging to the teleostean fish. isoelectric focusing of gamma-crystallin fraction obtained from gel-permeation chromatography revealed that it consists of multiple charge isomers of a protein species with a molecular mass of about 20 kda. to facilitate the cloning of gamma-crystallin gene, cdna was constructed from the poly(a)+mrna isolated from fresh lenses, and amplification by polymerase chain reaction ... | 1994 | 8037758 |
| toxicity identification evaluations for the investigation of fish kills: a case study. | a large fish-kill was observed in the river tajo during the spring-summer of 1991. the mortality was first detected between aranjuez and toledo, affecting several fish species. then it was slowly going downstream, affecting only carp (cyprinus carpio), reaching the spanish-portuguese border several months later. short-term toxicity tests on daphnia magna and in vitro cytotoxicity tests on rtg-2 cells were used as toxicity monitoring systems in water samples and different water fractions. the fis ... | 1994 | 8044634 |
| phylogenetic and ontogenetic study of neuropeptide y-containing nerves in the liver. | the distribution of neuropeptide y was investigated by light and electron microscopic immunohistochemistry in the liver of various vertebrates including the eel, carp, bullfrog, turtle, chicken, mouse, rat, guinea-pig, dog, monkey and human. the ontogenetic development of neuropeptide y was also studied in the mouse liver. in all species examined except the eel, neuropeptide y-like immunoreactivity was detected in nerve fibres. in the carp, bullfrog, turtle, chicken, mouse and rat, positive fibr ... | 1994 | 8045785 |
| [activity of lysosomal proteinases in carb tissue in aeromonas infection]. | the activity of major lysosomal proteinases (cathepsins b and d) increased in carp tissues infested with aeromonas hydrophila. the value and character of changes in the activity of cathepsins depended on the degree of infestation and the physiological state of fishes (normally fed or starved). | 1994 | 8047538 |
| characterisation of immunoglobulin-binding leucocytes in carp (cyprinus carpio l.). | this study demonstrates the immunoglobulin(ig)-binding capacity of ig-positive carp macrophages employing immunofluorescence and immunogold methods. these methods allow for the characterisation of the ig-binding cells. after internalisation of fluorescent- or gold-labelled ig (30 min at room temperature), most macrophages from the hindgut were able to bind added purified carp ig, which could be demonstrated clearly with a second fluorescent or gold label. in pronephros, an important haemopoietic ... | 1994 | 8050616 |
| creatine kinase, energy-rich phosphates and energy metabolism in heart muscle of different vertebrates. | maximal activities of creatine kinase, pyruvate kinase and cytochrome oxidase and total concentrations of creatine and phosphorylated adenylates were measured in cardiac muscle of hagfish, eight teleost species, frog, turtle, pigeon and rat. the ratio of creatine kinase to cytochrome oxidase with cytochrome oxidase as a rough estimate of aerobic capacity and cellular "energy turnover", was increased in myocardia of hagfish, turtle and crucian carp. these myocardia are likely to be frequently exp ... | 1994 | 8056878 |
| immunoreactive atrial natriuretic peptides in the oocyte. | 1. the presence and partial characterization of immunoreactive anp (iranp) in eggs were investigated using high performance liquid chromatography (hplc), immunohistochemistry and northern-blot hybridization in mammalian and nonmammalian vertebrates. 2. serial dilution curves of egg extracts from frogs and freshwater teleostean fishes (silver crusian carp and loach) were parallel to the standard curve of atriopeptin iii. rat oocytes also contained iranp (25-30 pg/egg). 3. the hplc profile of iran ... | 1993 | 8095876 |
| distribution of free d-serine in vertebrate brains. | free d-serine distribution in vertebrate brains was investigated. in various brain regions of the lower vertebrate species, carp, frog and chick, free d-serine levels were low. on the contrary, in the mammals, mouse, rat and bull, the contents of free d-serine were high in the forebrain (around 400 nmol/g wet weight, and the ratio of d-serine to l-serine, was d/l = 0.4), and low in the hindbrain. in developing mice, d-serine levels in the cerebrum increased with age and attained the adult level ... | 1994 | 8131079 |
| purification and characterization of urotensin ii from the brain of a teleost (trout, oncorhynchus mykiss) and an elasmobranch (skate, raja rhina). | peptides related to urotensin ii have been isolated in pure form from an extract of whole brain of a teleost, the rainbow trout (oncorhynchus mykiss) and of an elasmobranch, the longnose skate (raja rhina). the primary structure of the trout peptide [gly-gly-asn-ser-glu-cys-phe-trp-lys-tyr-cys-val] is similar to that of urotensin ii peptides isolated from the urophyses of other teleost fish. for example, trout urotensin ii contains only one amino acid substitution (thr4-->ser) compared with urot ... | 1993 | 8138107 |
| isolation of a carp complement protein homologous to mammalian factor d. | factor d of carp (cyprinus carpio) complement was purified to apparent homogeneity by a 4-step chromatographic procedure and examined for physicochemical and functional properties. carp factor d proved to be an alpha-globulin with a molecular mass of 29 kda and the serum concentration was estimated to be 6 micrograms/ml. the nh2-terminal amino acid sequence (30 residues including five unidentified positions) of carp factor d showed high homologies (57-60%) to those of mammalian factor d. neither ... | 1994 | 8152436 |
| [the protein level in bothriocephalus acheilognathi cestodes and their hosts, carp fingerlings, in experimental feeding and starving of the fish]. | | 1993 | 8152846 |
| a case-control study of oral cancer in beijing, people's republic of china. associations with nutrient intakes, foods and food groups. | a case-control study of oral cancer was conducted in beijing, people's republic of china to examine the association between dietary nutrient intake and risk of oral cancer, both in terms of estimated intake of nutrients and micro-nutrients, and in terms of specific foods and food groups. the study was hospital-based and controls were hospital in-patients matched for age and sex with the cases. the response rate for cases and controls was 100% and 404 case/control pairs were interviewed. the resu ... | 1993 | 8180577 |
| comparative studies on fish and bird insulin receptors. | the comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). the scatchard plots indicated the presence of two pools of binding sites both in fish and duck. these pools show inter-species differences in binding ability and the number of receptors. specific binding of insulin and the binding affinity are higher in duck than in fish. | 1993 | 8212960 |