| management of meloidogyne incognita on tomato with endophytic bacteria and fresh residue of wasabia japonica. | to characterize the nematicidal endophytic bacteria (neb) of wasabia japonica (wasabi) and evaluated the control efficacies of promising neb as well as fresh wasabi residue (fwr) against meloidogyne incognita on tomato. | 2014 | 24965702 |
| the cyclin-dependent kinase inhibitor krp6 induces mitosis and impairs cytokinesis in giant cells induced by plant-parasitic nematodes in arabidopsis. | in arabidopsis thaliana, seven cyclin-dependent kinase (cdk) inhibitors have been identified, designated interactors of cdks or kip-related proteins (krps). here, the function of krp6 was investigated during cell cycle progression in roots infected by plant-parasitic root-knot nematodes. contrary to expectations, analysis of meloidogyne incognita-induced galls of krp6-overexpressing lines revealed a role for this particular krp as an activator of the mitotic cell cycle. in accordance, krp6-overe ... | 2014 | 24963053 |
| elucidating the molecular bases of epigenetic inheritance in non-model invertebrates: the case of the root-knot nematode meloidogyne incognita. | root-knot nematodes of the genus meloidogyne are biotrophic plant parasites that exhibit different life cycles and reproduction modes, ranging from classical amphimixis to obligatory mitotic parthenogenesis (apomixis), depending on the species. meloidogyne incognita, an apomictic species, exhibits a worldwide distribution and a wide host range affecting more than 3000 plant species. furthermore, evidences suggest that apomixis does not prevent m. incognita from adapting to its environment in con ... | 2014 | 24936189 |
| a meloidogyne incognita effector is imported into the nucleus and exhibits transcriptional activation activity in planta. | root-knot nematodes are sedentary biotrophic endoparasites that maintain a complex interaction with their host plants. nematode effector proteins are synthesized in the oesophageal glands of nematodes and secreted into plant tissue through a needle-like stylet. effectors characterized to date have been shown to mediate processes essential for nematode pathogenesis. to gain an insight into their site of action and putative function, the subcellular localization of 13 previously isolated meloidogy ... | 2015 | 24863562 |
| isolation and characterisation of rhizosphere bacteria active against meloidogyne incognita, phytophthora nicotianae and the root knot-black shank complex in tobacco. | the use of dually antagonistic bacteria (dab) as alternatives to chemicals for biological control of disease complexes has received little attention. in this study targeting the meloidogyne incognita-phytophthora nicotianae complex, dab from the tobacco rhizosphere were identified and screened against the diseases caused by one or both pathogens in tobacco. | 2015 | 24799254 |
| histopathological response of lens culinaris roots towards root-knot nematode, meloidogyne incognito. | lens culinaris (lentil) is an important pulse crop. the yield of the crop is reduced if grown in root-knot nematode (meloidogyne incognita) infested field. meloidogyne incognita caused infection in primary and the secondary roots leading to the anomalies in the affected part of the root. the study revealed that the second stage juveniles (j2) of meloidogyne incognita entered the growing roots and their branches inter and intracellularly. the immediate response was hypertrophy and hyperplasia in ... | 2013 | 24498798 |
| chitosan-cinnamon beads enhance suppressive activity against rhizoctonia solani and meloidogyne incognita in vitro. | a novel chitosan-cinnamon bead carrier was prepared in this study. chitosan was mixed with cinnamon powder (cp) and cinnamon extract (ce) to obtain chitosan-cinnamon powder (ccp) beads and chitosan-cinnamon extracted (cce) beads, respectively. the potential antifungal and nematicidal activities of ccp and cce were investigated against rhizoctonia solani and meloidogyne incognita in vitro. relative antifungal activity of the ccp (5% cp) bead-treated r. solani was 30.9 and 23.9% after 1 and 2 day ... | 2014 | 24417978 |
| inheritance and mapping of mj-2, a new source of root-knot nematode (meloidogyne javanica) resistance in carrot. | root-knot nematodes limit carrot production around the world by inducing taproot forking and galling deformities that render carrots unmarketable. in warmer climates, meloidogyne javanica and meloidogyne incognita are most prevalent. in f2 and f3 progeny from the cross between an asian carrot resistant to m. javanica, pi 652188, and a susceptible carrot, resistance response was incompletely dominant with a relatively high heritability (h (2) = 0.78) and provided evidence for a single gene, desig ... | 2014 | 24336925 |
| effects of meloidogyne incognita and thielaviopsis basicola on cotton growth and root morphology. | effects of the root-knot nematode meloidogyne incognita and the fungal pathogen thielaviopsis basicola on cotton seedling growth and root morphology were evaluated in controlled environmental experiments. four pathogen treatments, including noninfested soil, soil infested with m. incognita, soil infested with t. basicola, and soil infested with both pathogens were evaluated at soil bulk densities (bds) of 1.25 and 1.50 g/cm(3). plant growth and the morphology of the root systems were evaluated 4 ... | 2014 | 24283540 |
| effects of chloroform extract of dryopteris crassirhizoma on the ultramicroscopic structures of meloidogyne incognita. | in our early experiments, the chloroform extract of d. crassirhizoma was demonstrated to contain the highest concentrations of total phloroglucinols among several extract fractions and possessed the most effective nematicidal activity. this study aimed to ascertain the ultrastructural changes in m. incognita after treatment with a d. crassirhizoma chloroform extract at 1 mg·ml⁻¹ for 24 h. it was found that the extract exhibited significant destructive effects on the worm's ultrastructure and cau ... | 2013 | 24282379 |
| utility of host delivered rnai of two fmrf amide like peptides, flp-14 and flp-18, for the management of root knot nematode, meloidogyne incognita. | root knot nematode, meloidogyne incognita, is an obligate sedentary endoparasite that infects a large number of crop species and causes substantial yield losses. non-chemical based control strategies for these nematodes are gaining importance. in the present study, we have demonstrated the significance of two fmrfamide like peptide genes (flp-14 and flp-18) for infection and development of resistance to m. incognita through host-derived rnai. the study demonstrated both in vitro and in planta va ... | 2013 | 24223228 |
| identification of novel target genes for safer and more specific control of root-knot nematodes from a pan-genome mining. | root-knot nematodes are globally the most aggressive and damaging plant-parasitic nematodes. chemical nematicides have so far constituted the most efficient control measures against these agricultural pests. because of their toxicity for the environment and danger for human health, these nematicides have now been banned from use. consequently, new and more specific control means, safe for the environment and human health, are urgently needed to avoid worldwide proliferation of these devastating ... | 2013 | 24204279 |
| [the participation of salicylic and jasmonic acids in genetic and induced resistance of tomato to meloidogyne incognita (kofoid and white, 1919)]. | salicylic (sa) and jasmonic (ja) acids are the best known mediators of signal systems in plants. in this investigation the participation and character of interactions between sa- and ja-signals under the induced and genetic resistance of plants to nematodes was investigated on the model system tomato (lycopersicon esculentum) and the root-knot nematode meloidogyne incognita. this study demonstrates that application of ja and sa to tomato foliage induces systemic effects that suppress root-knot n ... | 2016 | 24171314 |
| lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts mi-flp-18 expression to block infection. | tomato (solanum lycopersicum) crops can be severely damaged due to parasitism by the root-knot nematode (rkn) meloidogyne incognita, but are protected when intercropped with crown daisy (chrysanthemum coronarium l.). root exudate may be the determining factor for this protection. an experiment using pots linked by a tube and petri dish experiments were undertaken to confirm that tomato-crown daisy intercropping root exudate decreased the number of nematodes and alleviated nematode damage, and to ... | 2014 | 24170741 |
| expression of tomato salicylic acid (sa)-responsive pathogenesis-related genes in mi-1-mediated and sa-induced resistance to root-knot nematodes. | the expression pattern of pathogenesis-related genes pr-1, pr-2 and pr-5, considered as markers for salicylic acid (sa)-dependent systemic acquired resistance (sar), was examined in the roots and shoots of tomato plants pre-treated with sa and subsequently infected with root-knot nematodes (rkns) (meloidogyne incognita). pr-1 was up-regulated in both roots and shoots of sa-treated plants, whereas the expression of pr-5 was enhanced only in roots. the over-expression of pr-1 in the whole plant oc ... | 2014 | 24118790 |
| nematicidal activity of the essential oil of rhododendron anthopogonoides aerial parts and its constituent compounds against meloidogyne incognita. | hydrodistilled essential oil from rhododendron anthopogonoides maxim. (ericaceae) aerial parts was analysed by gas chromatography-mass spectrometry (gc-ms). a total of 42 compounds, accounting for 95.48% of the total oil, were identified. the main constituents of the essential oil were benzyl acetone (34.41%), nerolidol (10.19%), 1,4-cineole (8.41%), beta-caryophyllene (5.63%), gamma-elemene (5.10%), and spathulenol (3.06%). four constituents were isolated from the essential oil based on fractio ... | 2013 | 24066516 |
| enhanced levels of plant cell cycle inhibitors hamper root-knot nematode-induced feeding site development. | root-knot nematodes (rkn) are highly specialized, obligatory plant parasites. these animals reprogram root cells to form large, multinucleate, and metabolically active feeding cells (giant cells) that provide a continuous nutrient supply during 3-6 weeks of the nematode's life. the establishment and maintenance of physiologically fully functional giant cells are necessary for the survival of these nematodes. as such, giant cells may be useful targets for applying strategies to reduce damage caus ... | 2013 | 24056043 |
| nematicidal activity of mint aqueous extracts against the root-knot nematode meloidogyne incognita. | the nematicidal activity and chemical characterization of aqueous extracts and essential oils of three mint species, namely, mentha × piperita , mentha spicata , and mentha pulegium , were investigated. the phytochemical analysis of the essential oils was performed by means of gc-ms, whereas the aqueous extracts were analyzed by lc-ms. the most abundant terpenes were isomenthone, menthone, menthol, pulegone, and carvone, and the water extracts yielded mainly chlorogenic acid, salvianolic acid b, ... | 2013 | 24050256 |
| solanum torvum responses to the root-knot nematode meloidogyne incognita. | solanum torvum sw is worldwide employed as rootstock for eggplant cultivation because of its vigour and resistance/tolerance to the most serious soil-borne diseases as bacterial, fungal wilts and root-knot nematodes. the little information on solanum torvum (hereafter torvum) resistance mechanisms, is mostly attributable to the lack of genomic tools (e.g. dedicated microarray) as well as to the paucity of database information limiting high-throughput expression studies in torvum. | 2013 | 23937585 |
| the effect of transitional organic production practices on soilborne pests of tomato in a simulated microplot study. | the perceived risk of pest resurgence upon transition from conventional to organic-based farming systems remains a critical obstacle to expanding organic vegetable production, particularly where chemical fumigants have provided soilborne pest and disease control. microplots were used to study the effects of soil amendments and cropping sequences applied over a 2-year transitional period from conventional to organic tomato (solanum lycopersicum) cultivation on the incidence of bacterial wilt caus ... | 2013 | 23837543 |
| morphological and morphometrical characterization of meloidogyne incognita from different host plants in four districts of punjab, india. | the population of m. incognita, the root knot nematode (rkn) was found infesting five different host plants (okra, banana, sunflower, bottle gourd, and brinjal) out of 24 examined from four districts of punjab, india (gurdaspur, ludhiana, patiala, and hoshiarpur). morphological and morphometrical characterization indicated that in the case of mature female, the characters of body length and width, neck length, ratio 'a', anus to tail terminus (att), interphasmid distance (ipd), and perineal patt ... | 2013 | 23833327 |
| effects of soil compaction and meloidogyne incognita on cotton root architecture and plant growth. | the effects of a soil hardpan and meloidogyne incognita on cotton root architecture and plant growth were evaluated in microplots in 2010 and 2011. soil was infested with m. incognita at four different levels with or without a hardpan. the presence of a hardpan resulted in increased plant height, number of main stem nodes, and root fresh weight for cotton seedlings both years. meloidogyne incognita decreased height and number of nodes for seedlings in 2010. nematode infestation increased seedlin ... | 2013 | 23833326 |
| identification of a critical region in the drosophila ryanodine receptor that confers sensitivity to diamide insecticides. | anthranilic diamides, which include the new commercial insecticide, chlorantraniliprole, are an exciting new class of chemistry that target insect ryanodine receptors. these receptors regulate release of stored intracellular calcium and play a critical role in muscle contraction. as with insects, nematodes express ryanodine receptors and are sensitive to the plant alkaloid, ryanodine. however the plant parasitic nematode, meloidogyne incognita, is insensitive to anthranilic diamides. expression ... | 2013 | 23806522 |
| nematicidal activity of nonacosane-10-ol and 23a-homostigmast-5-en-3β-ol isolated from the roots of fumaria parviflora (fumariaceae). | two bioactive nematicidal phytochemicals, viz., nonacosane-10-ol and 23a-homostigmast-5-en-3β-ol, were isolated from the n-hexane fraction of the roots of fumaria parviflora through activity-guided isolation. the structures of the compounds were elucidated using ¹³c and ¹h nuclear magnetic resonance. activity of the two compounds against eggs and juveniles (j2s) of meloidogyne incognita was evaluated in vitro at the concentrations of 50, 100, 150, and 200 μg ml⁻¹. over 120 h of incubation, the c ... | 2013 | 23713689 |
| isolation of nematicidal triterpenoid saponins from pulsatilla koreana root and their activities against meloidogyne incognita. | pulsatilla koreana, a species endemic to korea, is an important herb used in traditional medicine to treat amoebic dysentery and malaria. in the present study, 23 oleanane-type triterpenoid saponins 1-23 and eight lupane-type triterpenoid saponins 24-31 were isolated from the roots of p. koreana. their structures were elucidated on the basis of spectroscopic data. the methanol extract and isolated compounds were next assessed for nematicidal activity against the root-knot nematode (meloidogyne i ... | 2013 | 23698044 |
| validation of reference genes aiming accurate normalization of qpcr data in soybean upon nematode parasitism and insect attack. | soybean pathogens and pests reduce grain production worldwide. biotic interaction cause extensive changes in plant gene expression profile and the data produced by functional genomics studies need validation, usually done by quantitative pcr. nevertheless, this technique relies on accurate normalization which, in turn, depends upon the proper selection of stable reference genes for each experimental condition. to date, only a few studies were performed to validate reference genes in soybean subj ... | 2013 | 23668315 |
| nematicidal activity of allylisothiocyanate from horseradish (armoracia rusticana) roots against meloidogyne incognita. | in recent years, there has been a great development in the search for new natural pesticides for crop protection aiming a partial or total replacement of currently used chemical nematicides. glucosinolate breakdown products are volatile and are therefore good candidates for nematodes fumigants. in this article, the methanol-aqueous extract (1:1, w/v) of horseradish (armoracia rusticana) fresh roots (mah) was in vitro tested for nematicidal activity against second stage (j2) meloidogyne incognita ... | 2013 | 23627288 |
| nematicidal activity of 3,4-dihydroxybenzoic acid purified from terminalia nigrovenulosa bark against meloidogyne incognita. | in this study, the 3,4-dihydroxybenzoic acid (3,4-dhba) from terminalia nigrovenulosa bark (tnb) was purified and its in vitro nematicidal activity was investigated against meloidogyne incognita. the purification of 3,4-dhba used a silica gel column and sephadex lh-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. structural identification of the 3,4-dhba was conducted using (1)h nuclear magnetic resonance (nmr), (13)c nmr, and liquid chromatog ... | 2015 | 23603737 |
| transcriptional changes of the root-knot nematode meloidogyne incognita in response to arabidopsis thaliana root signals. | root-knot nematodes are obligate parasites that invade roots and induce the formation of specialized feeding structures. although physiological and molecular changes inside the root leading to feeding site formation have been studied, very little is known about the molecular events preceding root penetration by nematodes. in order to investigate the influence of root exudates on nematode gene expression before plant invasion and to identify new genes potentially involved in parasitism, sterile r ... | 2013 | 23593446 |
| penetration, post-penetration development, and reproduction of meloidogyne incognita on cucumis melo var. texanus. | cucumis melo var. texanus, a wild melon commonly found in the southern united states and two accessions, burleson co. and mx 1230, expressed resistance to meloidogyne incognita in preliminary experiments. to characterize the mechanism of resistance, we evaluated root penetration, post-penetration development, reproduction, and emigration of m. incognita on these two accessions of c. melo var. texanus. additionally, we evaluated 22 accessions of c. melo var. texanus for their reaction against m. ... | 2013 | 23589661 |
| the relationship between environmental variables and response of cotton to nematicides. | nematicide/irrigation rate trials were conducted in texas (tx) in 2009 and 2010 in cotton grown at three irrigation rates, where irrigation rate (base (b), b - 33%, b + 33%) was the main plot and treatment (untreated check, aldicarb, and nematicide seed treatment (nst) and nst + aldicarb) were the subplots. aldicarb improved cotton lint yield with the base (medium) irrigation rate over the untreated check, but not at the b - 33% and b + 33% irrigation rates. in a second evaluation, 20 tests cond ... | 2013 | 23589654 |
| ectopic expression of kip-related proteins restrains root-knot nematode-feeding site expansion. | the development of nematode feeding sites induced by root-knot nematodes involves the synchronized activation of cell cycle processes such as acytokinetic mitoses and dna amplification. a number of key cell cycle genes are reported to be critical for nematode feeding site development. however, it remains unknown whether plant cyclin-dependent kinase (cdk) inhibitors such as the arabidopsis interactor/inhibitor of cdk (ick)/kip-related protein (krp) family are involved in nematode feeding site de ... | 2013 | 23574394 |
| chemical composition and nematicidal activity of essential oil of agastache rugosa against meloidogyne incognita. | the aim of this research was to determine the chemical composition and nematicidal activity of essential oil of agastache rugosa flowering aerial parts against the root knot nematode, meloidogyne incognita, and to isolate and identify any nematicidal constituents from the essential oil. the essential oil of a. rugosa aerial parts was obtained by hydrodistillation and analyzed by gc-fid and gc-ms. a total of 37 components of the essential oil were identified, with the principal compounds being me ... | 2013 | 23571530 |
| fine mapping and identification of candidate genes controlling the resistance to southern root-knot nematode in pi 96354. | meloidogyne incognita (kofoid and white) chitwood (mi) is the most economically damaging species of the root-knot nematode to soybean and other crops in the southern usa. pi 96354 was identified to carry a high level of resistance to galling and mi egg production. two quantitative trait locus (qtls) were found to condition the resistance in pi 96354 including a major qtl and a minor qtl on chromosome 10 and chromosome 18, respectively. to fine map the major qtl on chromosome 10, f5:6 recombinant ... | 2013 | 23568221 |
| a new nematicidal compound produced by streptomyces albogriseolus ha10002. | strain ha10002 was isolated from mangrove sediment collected from dongzhaigang mangrove reserve in hainan, china. it was selected with potent nematicidal activity and was identified as streptomyces albogriseolus. by bioassay-guided fractionation, a new active component a22-1(s1) against root-knot nematodes was separated from its fermentation broth. on the basis of spectroscopic analyses and comparison with the data from correlative literature, the structure of s1 was established to be 6'-methyl- ... | 2013 | 23444037 |
| multiyear evaluation of the durability of the resistance conferred by ma and rmia genes to meloidogyne incognita in prunus under controlled conditions. | root-knot nematodes (rkns) (meloidogyne spp.) are highly polyphagous pests that parasitize prunus crops in mediterranean climates. breeding for rkn-resistant prunus cultivars, as an alternative to the now-banned use of nematicides, is a real challenge, because the perennial nature of these trees increases the risk of resistance breakdown. the ma plum resistance (r) gene, with a complete spectrum, and the rmia peach r gene, with a more restricted spectrum, both provide total control of meloidogyn ... | 2013 | 23425239 |
| potent nematicidal activity of phthalaldehyde, salicylaldehyde, and cinnamic aldehyde against meloidogyne incognita. | the nematicidal activity of selected aromatic aldehydes was tested against the root knot nematode meloidogyne incognita. the most active aldehyde was phthalaldehyde (1) with an ec(50) value of 11 ± 6 mg/l followed by salicylaldehyde (2) and cinnamic aldehyde (3) with ec(50) values of 11 ± 1 and 12 ± 5 mg/l, respectively. on the other hand, structurally related aldehydes such as 2-methoxybenzaldehyde (21), 3,4-dimethoxybenzaldehyde, and vanillin (23) were not active at the concentration of 1000 m ... | 2013 | 23379671 |
| bacillus and streptomyces were selected as broad-spectrum antagonists against soilborne pathogens from arid areas in egypt. | plant protection via disease-suppressive bacteria in desert farming requires specific biological control agents (bcas) adapted to the unique arid conditions. we performed an ecological study of below-ground communities in desert farm soil and untreated desert soil, and based on these findings, selected antagonists were hierarchically evaluated. in contrast to the highly specific 16s rrna fingerprints of bacterial communities in soil and cultivated medicinal plants, internal transcribed spacer pr ... | 2013 | 23350560 |
| the amino acid permeases aap3 and aap6 are involved in root-knot nematode parasitism of arabidopsis. | the root-knot nematode, meloidogyne incognita, is an obligate parasite which depends entirely on the host plant for its nutrition. root-knot nematodes induce the formation of a highly specialized feeding site consisting of several giant cells surrounded by a network of vascular tissues. nutrients, including amino acids and sugars, are transferred apoplastically from the vascular tissues to the feeding site. using arabidopsis thaliana lacking the vascular-expressed amino acid permeases (aap) aap3 ... | 2013 | 23194341 |
| proteomic profiles of soluble proteins from the esophageal gland in female meloidogyne incognita. | meloidogyne incognita can infect multiple plant species. proteins synthesized in the esophageal glands and secreted through the stylet of plant parasitic nematodes play critical roles in the plant-nematode interactions. female m. incognita live for approximately 15days, embedded in a host plant, but their esophageal gland proteins have not yet been comprehensively analyzed. in this study, a new bacterium-contamination-resistant method for collecting soluble proteins from esophageal gland cells ( ... | 2012 | 23142006 |
| nematotoxic phenolic compounds from melia azedarach against meloidogyne incognita. | in the present study, evaluated was the paralysis activity of whole italian and algerian melia azedarach, commonly known as chinaberry, fruits and parts (seeds, wood, and kernels) against meloidogyne incognita second stage juveniles (j(2)). the paralysis activity was evaluated in vitro after 1 h and 1 day immersion periods of nematodes in test solutions. phenolic constituent components of the extracts were identified and quantified by high-performance liquid chromatography-mass spectrometry, whi ... | 2012 | 23136941 |
| potential antagonistic effects of nine natural fatty acids against meloidogyne incognita. | fatty acids, the essential components of life, were widely present in various seed cakes, gutter oil, and other resources. the objective of this study was to evaluate the potential antagonistic effects of nine fatty acids (fas) against meloidogyne incognita (root-knot nematodes). the results showed that butyric, caprylic, capric, lauric, myristic, palmitic, and oleic acids significantly reduced m. incognita reproduction, whereas cucumber (cucumus sativus) biomass was not adversely affected by th ... | 2012 | 23121218 |
| the arabidopsis f-box/kelch-repeat protein at2g44130 is upregulated in giant cells and promotes nematode susceptibility. | we report that the f-box/kelch-repeat protein at2g44130 is specifically induced by the root-knot nematode meloidogyne incognita during the initial stages of the initiation and maintenance of the feeding site. in addition, we show that the expression of this gene promotes susceptibility of infection because knocking down the f-box gene (at2g44130) drastically reduces nematode attraction to and infection of roots. in contrast, f-box overexpressing (oe) lines had a hypersusceptible phenotype, with ... | 2013 | 23075039 |
| comparative proteomic study between tuberous roots of light orange- and purple-fleshed sweetpotato cultivars. | this study compares the differences in proteomes expressed in tuberous roots of a light orange-fleshed sweetpotato (ipomoea batatas (l.) lam. cultivar yulmi) and a purple-fleshed sweetpotato cultivar (shinjami). more than 370 protein spots were reproducibly detected by two-dimensional gel electrophoresis, in which 35 spots were up-regulated (yulmi vs. shinjami) or uniquely expressed (only yulmi or shinjami) in either of the two cultivars. of these 35 protein spots, 23 were expressed in yulmi and ... | 2012 | 22794925 |
| control of root-knot nematodes on tomato in stone wool substrate with biological nematicides. | the efficacy of four biological nematicides on root-galling, root-knot nematode (meloidogyne incognita) reproduction, and shoot weight of tomato (solanum lycopersicum) grown in stone wool substrate or in pots with sandy soil was compared to an oxamyl treatment and a non-treated control. in stone wool grown tomato, avid® (a.i. abamectin) was highly effective when applied as a drench at time of nematode inoculation. it strongly reduced root-galling and nematode reproduction, and prevented a reduct ... | 2011 | 22791920 |
| virulence of meloidogyne incognita to expression of n gene in pepper. | four pepper genotypes classified as resistant and four pepper genotypes classified as susceptible to several avirulent populations of m. incognita were compared for their reactions against a population of meloidogyne incognita (chitwood) kofoid and white which had been shown to be virulent to resistant bell pepper (capsicum annuum) in preliminary tests. the virulent population of m. incognita originated from a commercial bell pepper field in california. the resistant pepper genotypes used in all ... | 2011 | 22791917 |
| nematicidal activity of 2-thiophenecarboxaldehyde and methylisothiocyanate from caper (capparis spinosa) against meloidogyne incognita. | new pesticides based on plant extracts have recently gained interest in the development of nontoxic crop protection chemicals. numerous research studies are focused on the isolation and identification of new active compounds derived from plants. in this manuscript we report about the use of the mediterranean species capparis spinosa as a potent natural nematicidal agent against the root knot nematodes meloidogyne incognita. leaves, stems, and caper buds of capparis spinosa were used to obtain th ... | 2012 | 22769561 |
| the map-1 gene family in root-knot nematodes, meloidogyne spp.: a set of taxonomically restricted genes specific to clonal species. | taxonomically restricted genes (trgs), i.e., genes that are restricted to a limited subset of phylogenetically related organisms, may be important in adaptation. in parasitic organisms, trg-encoded proteins are possible determinants of the specificity of host-parasite interactions. in the root-knot nematode (rkn) meloidogyne incognita, the map-1 gene family encodes expansin-like proteins that are secreted into plant tissues during parasitism, thought to act as effectors to promote successful roo ... | 2012 | 22719916 |
| whole-mount confocal imaging of nuclei in giant feeding cells induced by root-knot nematodes in arabidopsis. | • excellent visualization of nuclei was obtained here using a whole-mount procedure adapted to provide high-resolution images of large, irregularly shaped nuclei. the procedure is based on tissue clearing, and fluorescent staining of nuclear dna with the dye propidium iodide. • the method developed for standard confocal imaging was applied to large multicellular root swellings, named galls, induced in plant hosts by the root-knot nematode meloidogyne incognita. • here, we performed a functional ... | 2012 | 22616777 |
| virtual screening and in vitro assay of potential drug like inhibitors from spices against glutathione-s-transferase of meloidogyne incognita. | glutathione s-transferases (gsts) enzymes are critical antioxidant and detoxification system responsible for long-term existence of nematodes in host species. hence, 16 phytochemicals predicted and reported to have potential nematicidal activity have been docked to gst enzyme of meloidogyne incognita to assess their binding affinity and inhibitory activity. in vitro effects of these phytochemicals from in silico results have been done for validation of docking studies and efficacy in gst inhibit ... | 2012 | 22553389 |
| a root-knot nematode-secreted protein is injected into giant cells and targeted to the nuclei. | root-knot nematodes (rkns) are obligate endoparasites that maintain a biotrophic relationship with their hosts over a period of several weeks and induce the differentiation of root cells into specialized feeding cells. nematode effectors synthesized in the oesophageal glands and injected into the plant tissue through the syringe-like stylet certainly play a central role in these processes. in a search for nematode effectors, we used comparative genomics on expressed sequence tag (est) datasets t ... | 2012 | 22540860 |
| qtl analysis for transgressive resistance to root-knot nematode in interspecific cotton (gossypium spp.) progeny derived from susceptible parents. | the southern root-knot nematode (rkn, meloidogyne incognita) is a major soil-inhabiting plant parasite that causes significant yield losses in cotton (gossypium spp.). progeny from crosses between cotton genotypes susceptible to rkn produced segregants in subsequent populations which were highly resistant to this parasite. a recombinant inbred line (ril) population of 138 lines developed from a cross between upland cotton tm-1 (g. hirsutum l.) and pima 3-79 (g. barbadense l.), both susceptible t ... | 2012 | 22514682 |
| bioefficacy evaluation of controlled release formulations based on amphiphilic nano-polymer of carbofuran against meloidogyne incognita infecting tomato. | in the present investigation, the bioefficacy of developed carbofuran formulations, with peg-600 (7a, cp1) & peg-900 (7b, cp2) @ 5, 10 and 20 ppm, along with commercial formulation of carbofuran 3g (cp0) were evaluated against the root-knot nematode, meloidogyne incognita infecting tomato (cv. pusa ruby) in pot and field conditions. the bioefficacy data indicated that the formulations developed by utilizing polymers having peg - 900 (7b) as hydrophilic segment were effective even at 14 days post ... | 2012 | 22494375 |
| rapid and highly sensitive detection of single nematode via direct maldi mass spectrometry. | for the first time, we demonstrate the feasibility for the use of maldi-tof ms for rapid, direct and sensitive detection of single adult root-knot nematode, meloidogyne incognita and their second stage juvenile (j(2)). we have proposed simple pretreatment protocols and have demonstrated that the crushed and washed nematodes yielded better spectra. we also report the differentiation between the harmless and harmful stages of the nematode based on mass spectrometric profiling. peaks at m/z 4350, 4 ... | 2012 | 22483896 |
| nematicidal fluorescent pseudomonads for the in vitro and in vivo suppression of root knot (meloidogyne incognita) of capsicum annuum l. | considerable attention has been paid to plant-growth-promoting rhizobacteria (pgpr), especially the fluorescent group of pseudomonas species, as the best alternatives to chemicals for facilitating ecofriendly biological control of soil- and seedborne microorganisms. on the basis of their novel plant-growth-promoting attributes, two rhizobacteria pseudomonas aeruginosa vp1 and vp2 selected out of over 63 isolates from the rhizosphere of chilli (capsicum annuum) were identified as potential candid ... | 2012 | 22411906 |
| effect of abamectin on root-knot nematodes and tomato yield. | tomato growers in shandong province, china, commonly face heavy root-knot nematode infestations. current methods of control include cadusafos and methyl bromide (mebr), but alternative methods are required because of the high toxicity of these pesticides and the ecological risk of their use. therefore, abamectin soil applications were evaluated for their potential to control soil nematodes in a series of laboratory tests, greenhouse pot experiments and field trials. | 2012 | 22395950 |
| plant genes involved in harbouring symbiotic rhizobia or pathogenic nematodes. | the establishment and development of plant-microorganism interactions involve impressive transcriptomic reprogramming of target plant genes. the symbiont (sinorhizobium meliloti) and the root knot-nematode pathogen (meloidogyne incognita) induce the formation of new root organs, the nodule and the gall, respectively. using laser-assisted microdissection, we specifically monitored, at the cell level, medicago gene expression in nodule zone ii cells, which are preparing to receive rhizobia, and in ... | 2012 | 22360638 |
| rna interference-mediated growth control of the southern root-knot nematode meloidogyne incognita. | the southern root-knot nematode meloidogyne incognita causes huge crop losses. we investigated the effects of rna interference directed against the m. incognita posterior segregation-1 gene. the rnai resulted in a drastic reduction in the relative amount of pos-1 transcript. also, it reduced reproductive potential in terms of hatching rate from eggs, probably due to embryonic lethality. | 2012 | 22313763 |
| [control effects of ricinus communis extracts on meloidogyne incognita]. | toxicity test and pot experiment were conducted to study the nematocidal activity and control effects of ricinus communis extracts on meloidogyne incognita. the results showed that both the ricinine and the r. communis water extracts had high nematocidal activity. the ricinine at concentration 2 g x l(-1) and treated for 48 hours had the strongest nematocidal activity, leading to 91.5% of corrected mortality of m. incognita and with the lc50 being 0.6 g x l(-1), whereas the r. communis water ext ... | 2011 | 22303684 |
| sirnas trigger efficient silencing of a parasitism gene in plant parasitic root-knot nematodes. | expanding genomic data on plant pathogens open new perspectives for the development of specific and environment friendly pest management strategies based on the inhibition of parasitism genes that are essential for the success of infection. identifying such genes relies on accurate reverse genetics tools and the screening of pathogen knock-down phenotypes. root-knot nematodes are major cosmopolitan crop pests that feed on a wide range of host plants. small interfering rnas (sirnas) would provide ... | 2012 | 24704976 |
| differential expression of antioxidant enzymes and pr-proteins in compatible and incompatible interactions of cowpea (vigna unguiculata) and the root-knot nematode meloidogyne incognita. | this study aimed to evaluated the resistance and susceptibility of 10 cowpea cultivars to meloidogyne incognita in field studies and to analyze the kinetics of the enzymes superoxide dismutase, catalase, peroxidase, chitinase, β-1,3-glucanases and cystein proteinase inhibitors in the root system of two contrasting cowpea cultivars after inoculation with m. incognita. the cultivars ce-31 and frade preto were highly resistant; ce-28, ce-01, ce-315, ce-237, were very resistant; ce-70 and ce-216 wer ... | 2012 | 22153251 |
| arbuscular mycorrhizal fungi affect both penetration and further life stage development of root-knot nematodes in tomato. | the root-knot nematode meloidogyne incognita poses a worldwide threat to agriculture, with an increasing demand for alternative control options since most common nematicides are being withdrawn due to environmental concerns. the biocontrol potential of arbuscular mycorrhizal fungi (amf) against plant-parasitic nematodes has been demonstrated, but the modes of action remain to be unraveled. in this study, m. incognita penetration of second-stage juveniles at 4, 8 and 12 days after inoculation was ... | 2012 | 22147206 |
| [involvement of salicylic acid in induction of nematode resistance in plants]. | the role of salicylic acid (sa) as a possible signaling component in the case of the infection of plants with nematodes has been studied using a model system consisting of the tomato (lycopersicon esculentum (mill.) and race 1 of the gall eelworm meloidogyne incognita (kofoid and white, 1919; chitwood, 1949). the pre-planting sa treatment of tomato seeds results in an increased nematode resistance of susceptible tomato cultivars; the protective effect is higher in the case of sa combined with ch ... | 2016 | 22117420 |
| evidence of differences between the communities of arbuscular mycorrhizal fungi colonizing galls and roots of prunus persica infected by the root-knot nematode meloidogyne incognita. | arbuscular mycorrhizal fungi (amf) play important roles as plant protection agents, reducing or suppressing nematode colonization. however, it has never been investigated whether the galls produced in roots by nematode infection are colonized by amf. this study tested whether galls produced by meloidogyne incognita infection in prunus persica roots are colonized by amf. we also determined the changes in amf composition and biodiversity mediated by infection with this root-knot nematode. dna from ... | 2011 | 21984233 |
| microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon. | microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ... | 2011 | 23431109 |
| systemic nematicidal activity of fluensulfone against the root-knot nematode meloidogyne incognita on pepper. | fluensulfone, a new nematicide of the fluoroalkenyl group, has proved to be very effective in controlling root-knot nematodes, meloidogyne spp., by soil application. the systemic activity of this compound against m. incognita on peppers via soil drenching and foliar spray was evaluated. | 2012 | 21842527 |
| multiplex pcr for the simultaneous identification and detection of meloidogyne incognita, m. enterolobii, and m. javanica using dna extracted directly from individual galls. | meloidogyne incognita, m. enterolobii, and m. javanica are the most widespread species of root-knot nematodes in south china, affecting many economically important crops, ornamental plants, and fruit trees. in this study, one pair of meloidogyne universal primers was designed and three pairs of species-specific primers were employed successfully to rapidly detect and identify m. incognita, m. enterolobii, and m. javanica by multiplex polymerase chain reaction (pcr) using dna extracted from indiv ... | 2011 | 21770774 |
| nematocidal flavone-c-glycosides against the root-knot nematode (meloidogyne incognita) from arisaema erubescens tubers. | a screening of several chinese medicinal herbs for nematicidal properties showed that arisaema erubescens (wall.) schott tubers possessed significant nematicidal activity against the root-knot nematode (meloidogyne incognita). from the ethanol extract, two nematicidal flavone-c-glycosides were isolated by bioassay-guided fractionation. the compounds were identified as schaftoside and isoschaftoside on the basis of their phytochemical and spectral data. schaftoside and isoschaftoside possessed st ... | 2011 | 21694672 |
| aliphatic ketones from ruta chalepensis (rutaceae) induce paralysis on root knot nematodes. | this paper reports on the use of ruta chalepensis l. extracts as a potential nematicide against root knot nematodes meloidogyne incognita and meloidogyne javanica . the essential oil (reo) and methanol extract (rme) of r. chalepensis were tested against second-stage juveniles, with reo inducing paralysis in both species (ec(50/1d) = 77.5 and 107.3 mg/l) and rme being selective for m. incognita (ec(50/1d) = 1001 mg/l). chemical characterization of extracts was done by means of gc-ms and lc-ms, re ... | 2011 | 21631118 |
| elevated co2 influences nematode-induced defense responses of tomato genotypes differing in the ja pathway. | rising atmospheric co(2) concentrations can affect the induced defense of plants against chewing herbivores but little is known about whether elevated co(2) can change the induced defense of plants against parasitic nematodes. this study examined the interactions between the root-knot nematode meloidogyne incognita and three isogenic tomato (lycopersicon esculentum) genotypes grown under ambient (390 ppm) and elevated (750 ppm) co(2) in growth chambers. in a previous study with open-top chambers ... | 2011 | 21629688 |
| chemical constituents of cordia latifolia and their nematicidal activity. | following nematicidal activity-guided isolation studies on the fruits, bark, and leaves of cordia latifolia, two new constituents, cordinoic acid (=11-oxours-12-ene-23,28-dioic acid; 1) and cordicilin (=2-{[(e)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxy}-3-[4-hydroxy-3-(stearoyloxy)phenyl]propanoic acid; 2) were isolated from the stem and leaves, respectively, together with nine known compounds, namely cordioic and cordifolic acid from the stem bark, latifolicin a-d and rosmarinic acid from the fru ... | 2011 | 21560233 |
| effect of methomyl and oxamyl soil applications on early control of nematodes and insects. | methomyl is a widely used carbamate insecticide that has traditionally been applied as a foliar spray. more recently, methomyl has been labeled as a soil application via drip chemigation. not much is known about the insecticidal and nematicidal potential of soil-applied methomyl. methomyl soil applications were evaluated for their potential to control soil nematodes and foliar insect pests in a series of lab and greenhouse tests. | 2011 | 21438120 |
| identifying discriminative classification-based motifs in biological sequences. | identification of conserved motifs in biological sequences is crucial to unveil common shared functions. many tools exist for motif identification, including some that allow degenerate positions with multiple possible nucleotides or amino acids. most efficient methods available today search conserved motifs in a set of sequences, but do not check for their specificity regarding to a set of negative sequences. | 2011 | 21372086 |
| potential use of cucumber (cucumis sativus l.) endophytic fungi as seed treatment agents against root-knot nematode meloidogyne incognita. | seed treatment with endophytic fungi has been regarded as an effective method for plant parasitic nematode control. endophytic fungi from cucumber seedlings were isolated and screened for their potential to be used as seed treatment agents against meloidogyne incognita. among the 294 isolates screened, 23 significantly reduced galls formed by m. incognita in greenhouse test. the 10 most effective isolates were fusarium (5), trichoderma (1), chaetomium (1), acremonium (1), paecilomyces (1), and p ... | 2011 | 21370507 |
| mustard seed meal mixtures: management of meloidogyne incognita on pepper and potential phytotoxicity. | meals produced when oil is extracted from seeds in the brassicaceae have been shown to suppress weeds and soilborne pathogens. these seed meals are commonly used individually as soil amendments; the goal of this research was to evaluate seed meal mixes of brassica juncea (bj) and sinapis alba (sa) against meloidogyne incognita. seed meals from bj 'pacific gold' and sa 'idagold' were tested alone and in combinations to determine rates and application times that would suppress m. incognita on pepp ... | 2011 | 22791910 |
| synergistic and antagonistic interactions of terpenes against meloidogyne incognita and the nematicidal activity of essential oils from seven plants indigenous to greece. | biorational means for phytonematode control were studied within the context of an increasingly ecofriendly pest management global approach. the nematicidal activity and the chemical composition of essential oils (eos) isolated from seven plants grown in greece and ten selected compounds extracted from them against second-stage juveniles (j2) of meloidogyne incognita (kof. & white) chitwood were evaluated using juvenile paralysis experiments. additionally, synergistic and antagonistic interaction ... | 2011 | 21308960 |
| expression profile of jasmonic acid-induced genes and the induced resistance against the root-knot nematode (meloidogyne incognita) in tomato plants (solanum lycopersicum) after foliar treatment with methyl jasmonate. | we investigated what gene(s) in the plant roots have the positive role against repressing root-knot nematode (rkn) infection. we investigated the interaction between rkn infection and gene expression in the plant roots induced by methyl jasmonate (meja). we focused on the induced resistance response and the duration after foliar treatment with meja of 0.1, 0.5, 1.0, and 5.0mm at 1, 24, 48, and 72h prior to the inoculation of rkn. as a result, the foliar treatment with meja at 0.5mm or higher con ... | 2011 | 21216026 |
| evolution of nematode-resistant mi-1 gene homologs in three species of solanum. | plants have evolved several defense mechanisms, including resistance genes. resistance to the root-knot nematode meloidogyne incognita has been found in wild plant species. the molecular basis for this resistance has been best studied in the wild tomato solanum peruvianum and it is based on a single dominant gene, mi-1.2, which is found in a cluster of seven genes. this nematode attacks fiercely several crops, including potatoes. the genomic arrangement, number of copies, function and evolution ... | 2011 | 21207065 |
| identification of plant-parasitism genes in nematodes in silico screening and in vivo validation in meloidogyne incognita. | | 2011 | 22696951 |
| peroxiredoxins from the plant parasitic root-knot nematode, meloidogyne incognita, are required for successful development within the host. | root-knot nematodes, meloidogyne spp., are sedentary biotrophic parasites which are able to infest > 2000 plant species. after root invasion they settle sedentarily inside the vascular cylinder and maintain a compatible interaction for up to 8 weeks. plant cells respond to pathogen attacks by producing reactive oxygen species (ros). these ros, in particular hydroperoxides, are important regulators of host-parasite interactions and partly govern the success or failure of disease. ros producing an ... | 2011 | 21145323 |
| inheritance of resistance to meloidoygne incognita in primitive cotton accessions from mexico. | few sources of resistance to root-knot nematodes (meloidogyne incognita) in upland cotton (gossypium hirsutum) have been utilized to develop resistant cultivars, making this resistance vulnerable to virulence in the pathogen population. the objectives of this study were to determine the inheritance of resistance in five primitive accessions of g. hirsutum (tx1174, tx1440, tx2076, tx2079, and tx2107) and to determine allelic relations with the genes for resistance in the genotypes clevewilt-6 (cw ... | 2010 | 22736869 |
| data-mining of the meloidogyne incognita degradome and comparative analysis of proteases in nematodes. | proteases perform essential physiological functions in all living organisms. in parasitic helminths, they are of particular importance for tissue penetration, digestion of host tissues for nutrition, and evasion of host immune responses. the recent availability of the genome sequence of the nematode meloidogyne incognita has allowed the analysis of the protease repertoire of this major crop pathogen. the m. incognita degradome consists of at least 334 proteases that are distributed into 43 famil ... | 2011 | 20951198 |
| nematicidal carboxylic acids and aldehydes from melia azedarach fruits. | melia azedarach is a species gaining scientific interest mostly concerning its range of biological activities against agricultural target pests. the nematicidal melia methanol extract (mme) obtained from the fruits, acting against the phytonematode meloidogyne incognita , is herein reported to contain hexadecanoic, acetic, and hexanoic acids as well as furfural, 5-hydroxymethylfurfural, 5-methylfurfural, and furfurol. all compounds were tested individually for nematicidal activity against the ne ... | 2010 | 20925380 |
| geostatistical modeling of the spatial variability and risk areas of southern root-knot nematodes in relation to soil properties. | identifying the spatial variability and risk areas for southern root-knot nematode [meloidogyne incognita (kofoid & white) chitwood] (rkn) is key for site-specific management (ssm) of cotton (gossypium hirsutum l.) fields. the objectives of this study were to: (i) determine the soil properties that influence rkn occurrence at different scales; and (ii) delineate risk areas of rkn by indicator kriging. the study site was a cotton field located in the southeastern coastal plain region of the usa. ... | 2010 | 20717481 |
| fine mapping qmi-c11 a major qtl controlling root-knot nematodes resistance in upland cotton. | the identification and utilization of a high-level of host plant resistance is the most effective and economical approach to control root-knot nematode (meloidogyne incognita). in an earlier study, we identified a major quantitative trait locus (qtl) for resistance to root-knot nematode in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. the qtl is located in a 12.9-cm interval flanked by the two ssr markers cir069 and cir316 on the distal segment of chromos ... | 2010 | 20680610 |
| ssr markers closely associated with genes for resistance to root-knot nematode on chromosomes 11 and 14 of upland cotton. | molecular markers closely linked to genes that confer a high level of resistance to root-knot nematode (rkn) [meloidogyne incognita (kofoid & white) chitwood] in cotton (gossypium hirsutum l.) germplasm derived from auburn 623 rnr would greatly facilitate cotton breeding programs. our objectives were to identify simple sequence repeat (ssr) markers linked to rkn resistance quantitative trait loci (qtl) and map these markers to specific chromosomes. we developed three recombinant inbred line (ril ... | 2010 | 20607210 |
| phytochemistry and nematicidal activity of the essential oils from 8 greek lamiaceae aromatic plants and 13 terpene components. | eight essential oils (eos) as well as 13 single terpenes were studied for their nematicidal activity against meloidogyne incognita , for three immersion periods (24, 48, and 96 h). the eos were isolated from eight greek lamiaceae species: melissa officinalis , sideritis clandestina , origanum dictamnus , ocimum basilicum , mentha pulegium , origanum vulgare , vitex agnus castus , and salvia officinalis . the eos nematicidal activity was correlated to their chemical composition as well as to the ... | 2010 | 20527965 |
| elevated co2 changes the interactions between nematode and tomato genotypes differing in the ja pathway. | interactions between the root-knot nematode meloidogyne incognita and three isogenic tomato (lycopersicon esculentum) genotypes were examined when plants were grown under ambient (370 ppm) and elevated (750 ppm) co2. we tested the hypothesis that, defence-recessive genotypes tend to allocate 'extra' carbon (relative to nitrogen) to growth under elevated co2, whereas defence-dominated genotypes allocate extra carbon to defence, and thereby increases the defence against nematodes. for all three ge ... | 2010 | 20519018 |
| combining maxratio analysis with real-time pcr and its potential application for the prediction of meloidogyne incognita in field samples. | diagnosing and quantifying plant-parasitic nematodes is critical for efficient nematode management. several studies have been performed intending to demonstrate nematode quantification via real-time quantitative pcr. however, most of the studies used dilution of dna templates to make standard curves, while few studies used samples with different nematode numbers to make the standard curve, resulting in a high standard error. the objective of the present study was to develop a high quality standa ... | 2010 | 22736853 |
| the potential of five winter-grown crops to reduce root-knot nematode damage and increase yield of tomato. | broccoli (brassica oleracea), carrot (daucus carota), marigold (tagetes patula), nematode-resistant tomato (solanum lycopersicum), and strawberry (fragaria ananassa) were grown for three years during the winter in a root-knot nematode (meloidogyne incognita) infested field in southern california. each year in the spring, the tops of all crops were shredded and incorporated in the soil. amendment with poultry litter was included as a sub-treatment. the soil was then covered with clear plastic for ... | 2010 | 22736848 |
| strip-tilled cover cropping for managing nematodes, soil mesoarthropods, and weeds in a bitter melon agroecosystem. | a field trial was conducted to examine whether strip-tilled cover cropping followed by living mulch practice could suppress root-knot nematode (meloidogyne incognita) and enhance beneficial nematodes and other soil mesofauna, while suppressing weeds throughout two vegetable cropping seasons. sunn hemp (sh), crotalaria juncea, and french marigold (mg), tagetes patula, were grown for three months, strip-tilled, and bitter melon (momordica charantia) seedlings were transplanted into the tilled stri ... | 2010 | 22736847 |
| effect of mowing cotton stalks and preventing plant re-growth on post-harvest reproduction of meloidogyne incognita. | the southern root-knot nematode (meloidogyne incognita) is a major parasite of cotton in the u.s., and management tactics for this nematode attempt to minimize population levels. we compared three post-harvest practices for their ability to reduce nematode population levels in the field, thereby reducing initial nematode population for the next year's crop. the three practices tested were: 1) chemical defoliation before harvest plus cutting cotton stalks after harvest, 2) chemical defoliation pl ... | 2010 | 22736845 |
| nematicidal natural products from the aerial parts of buddleja crispa. | studies on the aerial parts of buddleja crispa yielded 13 known compounds, nonyl benzoate, hexyl p-hydroxy-cinnamate, ginipin, gardiol, 1-heptacosanol, steroidal galactoside (22 r)-stigmasta-7,9 (11)-dien-22 beta-ol-3beta-o-beta-d-galactopyranoside, 3-methoxy benzoic acid, beta-sitosterol and ursolic acid. besides this two iridoid galactosides buddlejosides a, buddlejosides b and a benzofuran-type sesquiterpene buddlejone have been isolated from the etoac fraction of b. crispa. together with the ... | 2010 | 20461624 |
| short interfering rna-mediated knockdown of drosha and pasha in undifferentiated meloidogyne incognita eggs leads to irregular growth and embryonic lethality. | micro-(mi)rnas play a pivotal role in the developmental regulation of plants and animals. we reasoned that disruption of normal heterochronic activity in differentiating meloidogyne incognita eggs may lead to irregular development, lethality and by extension, represent a novel target for parasite control. on silencing the nuclear rnase iii enzyme drosha, a critical effector of mirna maturation in animals, we found a significant inhibition of normal development and hatching in short interfering ( ... | 2010 | 20398669 |
| biocatalytic amidation of carboxylic acids and their antinemic activity. | a series of novel n-alkyl substituted amides, synthesized by enzyme catalysis, were evaluated against root-knot nematode, meloidogyne incognita and found to have potential antinemic activity. the corresponding amides were prepared by the condensation of equimolar amounts of carboxylic acids with different alkyl amines in the presence of candida antarctica lipase at 60-90 degrees c in 16-20 h. the reactions were carried out in a non - solvent system without the use of any activating agents. all t ... | 2010 | 20390959 |
| in vitro comparison of protease activities in preparations from free-living (panagrellus redivivus) and plant-parasitic (meloidogyne incognita) nematodes using fmrfa and fmrfa-like peptides as substrates. | extracts prepared from the microbivorous nematode panagrellus redivivus and the plant-parasitic nematode meloidogyne incognita were used to provide general protease activities for peptide substrate screening and species comparisons. each extract was evaluated for its ability to degrade a broad range of nematode fmrfamide-like peptides (flps), key regulatory messengers governing nematode growth and development. clear quantitative differences between the two extracts were observed using fmrfamide ... | 2010 | 20334714 |
| nematicidal natural products from the aerial parts of rubus niveus. | studies on the aerial parts of rubus niveus yielded six known compounds, 3,5-dihydroxy benzoic acid c(7)h(6)o(4), (1), gallic acid c(7)h(6)o(5) (2), ethyl galactoside (3), oleanolic acid (4), beta-sitosterol (5) and 3-o-[beta-d-galactopyranosyl-(12)-d-glucopyranoside (6). besides this, a gallic acid derivative with methyl substitution was synthesised as tetramethyl gallate (3). together with this derivative, compounds 1, 2, the alcohol soluble, chloroform soluble and petroleum ether soluble extr ... | 2010 | 20306362 |
| effects of a novel pathogenesis-related class 10 (pr-10) protein from crotalaria pallida roots with papain inhibitory activity against root-knot nematode meloidogyne incognita. | a novel pathogenesis-related class 10 (pr-10) protein with papain inhibitory activity, named cppri, was purified from crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid chromatographies (hplcs). cppri is made up of a single polypeptide chain with a m(r) of 15 kda, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this protein exhibited a k(i) value of 1.8 x 10(-9) m and operates via a noncompetit ... | 2010 | 20199085 |
| microwave assisted solvent-free synthesis and biological activities of novel imines (schiff bases). | twelve new ortho-hydroxyketimines were synthesized by conventional as well as microwave method and evaluated for their antinemic activity against meloidogyne incognita [(kofoid and white) chitwood]. conventional methods for synthesis of schiff bases require refluxing at 140 degrees c of the reactants in different solvents for at least 24 h or more, where as the microwave-assisted synthesis has brought down the reaction time from 24 h to 1 minute. the procedure reported is simple as it does not r ... | 2009 | 20183046 |
| additive effects of plant expressed double-stranded rnas on root-knot nematode development. | ectopically expressed double-stranded rnas (dsrnas) have recently been shown to suppress parasitic success of meloidogyne spp. in plants. we have targeted two genes from the root-knot nematode meloidogyne incognita; a dual oxidase gene implicated in the tyrosine cross-linking of the developing cuticle and a subunit of signal peptidase, a protein complex required for the processing of secreted proteins. while these genes are involved in different aspects of nematode development, the phenotypic co ... | 2010 | 20100489 |