| correlation of the genotoxic activation and kinetic properties of salmonella enterica serovar typhimurium nitroreductases snra and cnr with the redox potentials of nitroaromatic compounds and quinones. | bacterial nitroreductases (nrs) catalyse the oxygen-insensitive reduction of several nitro-substituted compounds and quinones. snra and cnr nrs have been previously identified in salmonella enterica serovar typhimurium; they reduce several environmental nitro compounds that display mutagenic activity in the ames test. although some of their biochemical properties have been reported, the substrate specificity of each protein over mutagenic nitro compounds is unknown; even more, the possible relat ... | 2010 | 20118186 |
| flagellated but not hyperfimbriated salmonella enterica serovar typhimurium attaches to and forms biofilms on cholesterol-coated surfaces. | the asymptomatic, chronic carrier state of salmonella enterica serovar typhi occurs in the bile-rich gallbladder and is frequently associated with the presence of cholesterol gallstones. we have previously demonstrated that salmonellae form biofilms on human gallstones and cholesterol-coated surfaces in vitro and that bile-induced biofilm formation on cholesterol gallstones promotes gallbladder colonization and maintenance of the carrier state. random transposon mutants of s. enterica serovar ty ... | 2010 | 20118264 |
| role of the c-terminal cytoplasmic domain of flha in bacterial flagellar type iii protein export. | for construction of the bacterial flagellum, many of the flagellar proteins are exported into the central channel of the flagellar structure by the flagellar type iii protein export apparatus. flha and flhb, which are integral membrane proteins of the export apparatus, form a docking platform for the soluble components of the export apparatus, flih, flii, and flij. the c-terminal cytoplasmic domain of flha (flha(c)) is required for protein export, but it is not clear how it works. here, we analy ... | 2010 | 20118266 |
| morphologic and cytokine profile characterization of salmonella enterica serovar typhimurium infection in calves with bovine leukocyte adhesion deficiency. | the role of neutrophils in the pathogenesis of salmonella enterica typhimurium-induced ruminant and human enteritis and diarrhea has yet to be characterized with in vivo models. to address this question, the in vivo bovine ligated ileal loop model of nontyphoidal salmonellosis was used in calves with the naturally occurring bovine leukocyte adhesion deficiency (blad) mutation whose neutrophils are unable to extravasate and infiltrate the extravascular matrix. data obtained from 4 blad holstein c ... | 2010 | 20118318 |
| salmonella sdia recognizes n-acyl homoserine lactone signals from pectobacterium carotovorum in vitro, but not in a bacterial soft rot. | genomes of salmonella enterica isolates, including those linked to outbreaks of produce-associated gastroenteritis, contain sdia, which encodes a receptor of n-acyl homoserine lactones (ahl). ahl are the quorum-sensing signals used by bacteria to coordinately regulate gene expression within -their populations. because s. enterica does not produce its own ahl, sdia is hypothesized to function in the interspecies cross-talk with ahl-producing bacteria. under laboratory conditions, s. enterica resp ... | 2010 | 20121449 |
| bacterial chemoreceptors: providing enhanced features to two-component signaling. | bacteria perform chemotaxis utilizing core two-component signaling systems to which have been added enhanced features of signal amplification, sensory adaptation, molecular memory and high sensitivity over a wide dynamic range. chemoreceptors are central to the enhancements. these transmembrane homodimers associate in trimers and in clusters of signaling complexes containing from a few to thousands of receptors. receptor homodimers couple ligand occupancy and adaptational modification to transme ... | 2010 | 20122866 |
| bacterial guanine nucleotide exchange factors sope-like and wxxxe effectors. | subversion of rho family small gtpases, which control actin dynamics, is a common infection strategy used by bacterial pathogens. in particular, salmonella enterica serovar typhimurium, shigella flexneri, enteropathogenic escherichia coli (epec), and enterohemorrhagic escherichia coli (ehec) translocate type iii secretion system (t3ss) effector proteins to modulate the rho gtpases rhoa, cdc42, and rac1, which trigger formation of stress fibers, filopodia, and lamellipodia/ruffles, respectively. ... | 2010 | 20123714 |
| enterococcus mundtii st4sa and lactobacillus plantarum 423 alleviated symptoms of salmonella infection, as determined in wistar rats challenged with salmonella enterica serovar typhimurium. | wistar rats were administered daily with lactobacillus plantarum 423 and enterococcus mundtii st4sa through intragastric gavage (1 x 10(8) cfu of each strain and a combination of the two strains). sterile saline was used as placebo. after 7 days, the animals were challenged by infection with 2 x 10(8) cfu salmonella enterica serovar typhimurium. after 1 day of treatment with l. plantarum 423 and e. mundtii st4sa, the feed and water intake, and body weight of the rats increased. the faecal moistu ... | 2010 | 20127245 |
| [development and validation of a multiplex polymerase chain reaction for molecular identification of salmonella enterica serogroups b, c2, d and e]. | the scheme kauffman-white (kw) for serotyping of salmonella recognizes 46 o antigens, and 119 h antigens, thereby permitting the characterization of 2541 serotypes. the serotyping is a useful epidemiological tool in identifying circulating serotypes and to characterize outbreaks. however, the method presents technical limitations, difficulty in interpretation of results and high costs. | 2009 | 20128349 |
| effects of dam and/or seqa mutations on the fatty acid and phospholipid membrane composition of salmonella enterica serovar typhimurium. | we examined the phospholipids (phls) and the membrane fatty acid (fa) composition in salmonella enterica serovar typhimurium dam and/or seqa mutants. phosphatidylglycerol, phosphatidylethanolamine (pe), and cardiolipin (cl) are the major phls present in all the strains and accounted for greater than 95% of the total lipid phosphorus. phosphatidic acid and phosphatidylserine are the minor ones. the seqa mutant showed a decrease in pe and an increase in cl and phosphatidylglycerol proportion compa ... | 2010 | 20132031 |
| curtailed short-term and long-term survival following infection with non-typhoid salmonella in israel. | among bacterial foodborne pathogens, non-typhoid salmonella enterica (nts) is a leading cause of death worldwide. this study assessed short-term and long-term mortality following nts infection in israel, and evaluated the effects of age, sex, source of isolation and different serotypes on mortality. the source of data was a national registry of nts isolates submitted to the salmonella reference center, government central laboratories, in jerusalem, israel, during 1997-2006. vital status was deri ... | 2011 | 20132249 |
| the role of the flik molecular ruler in hook-length control in salmonella enterica. | a molecular ruler, flik, controls the length of the flagellar hook. flik measures hook length and catalyses the secretion-substrate specificity switch from rod-hook substrate specificity to late substrate secretion, which includes the filament subunits. here, we show normal hook-length control and filament assembly in the complete absence of the c-ring thus refuting the previous 'cup' model for hook-length control. mutants of c-ring components, which are reported to produce short hooks, show a r ... | 2010 | 20132451 |
| carvacrol and cinnamaldehyde inactivate antibiotic-resistant salmonella enterica in buffer and on celery and oysters. | the emergence of antibiotic-resistant salmonella is of concern to food processors. the objective of this research was to identify antimicrobial activities of cinnamaldehyde and carvacrol against antibiotic-resistant salmonella enterica in phosphate-buffered saline (pbs) and on celery and oysters. twenty-three isolates were screened for resistance to seven antibiotics. two resistant and two susceptible strains were chosen for the study. s. enterica cultures (10(5) cfu/ml) were added to different ... | 2010 | 20132667 |
| use of electron beam radiation for the reduction of salmonella enterica serovars typhimurium and tennessee in peanut butter. | peanut butter and peanut paste products were implicated as the vehicle of contamination in an outbreak of salmonella typhimurium, which began in september 2008, and in the november 2006 outbreak of salmonella tennessee. therefore, this study evaluated the effectiveness of electron beam (e-beam) radiation for the reduction of salmonella serovars tennessee (atcc 10722) and typhimurium (atcc 14028) in creamy peanut butter. each strain was studied independently. peanut butter samples were inoculated ... | 2010 | 20132682 |
| dynamics of intracellular bacterial replication at the single cell level. | several important pathogens cause disease by surviving and replicating within host cells. bacterial proliferation is the product of both replication and killing undergone by the population. however, these processes are difficult to distinguish, and are usually assessed together by determination of net bacterial load. in addition, measurement of net load does not reveal heterogeneity within pathogen populations. this is particularly important in persistent infections in which slow or nongrowing b ... | 2010 | 20133586 |
| an oligonucleotide microarray to characterize multidrug resistant plasmids. | many of the enterobacteriaceae carry multiple drug resistance (mdr) genes on large plasmids of replicon type inc a/c and inc h1. it is important to understand the transmission of these mdr plasmids because the genes they carry can affect the outcome of antimicrobial therapy. the aim of this study was to design a microarray with oligonucleotide probes for every gene in the six inc a/c and one inc h1 plasmids of interest while representing all redundant sequences only once. the microarray is print ... | 2010 | 20138094 |
| genetic modification of the salmonella membrane physical state alters the pattern of heat shock response. | it is now recognized that membranes are not simple physical barriers but represent a complex and dynamic environment that affects membrane protein structures and their functions. recent data emphasize the role of membranes in sensing temperature changes, and it has been shown that the physical state of the plasma membrane influences the expression of a variety of genes such as heat shock genes. it has been widely shown that minor alterations in lipid membranes are critically involved in the conv ... | 2010 | 20139186 |
| relevance of dna alkylation damage repair systems in salmonella enterica virulence. | systematic inactivation of pathways involved in dna alkylation damage repair demonstrated that inactivation of the ada, ogt, tag, uvra, and mfd genes is required to detect a salmonella enterica virulence decrease. furthermore, the fitness of s. enterica, defective in these genes, is lowered only when the bacterium is orally, but not intraperitoneally, inoculated. | 2010 | 20139190 |
| changes in membrane fluid state and heat shock response cause attenuation of virulence. | so far attenuation of pathogens has been mainly obtained by chemical or heat treatment of microbial pathogens. recently, live attenuated strains have been produced by genetic modification. we have previously demonstrated that in several prokaryotes as well as in yeasts and mammalian cells the heat shock response is controlled by the membrane physical state (mps). we have also shown that in salmonella enterica serovar typhimurium lt2 (salmonella typhimurium) overexpression of a delta(12)-desatura ... | 2010 | 20139193 |
| synthesis of ag/cnt hybrid nanoparticles and fabrication of their nylon-6 polymer nanocomposite fibers for antimicrobial applications. | ag-coated cnts hybrid nanoparticles (ag/cnts) were prepared by ultrasonic irradiation of dimethylformamide (dmf) and silver (i) acetate precursors in the presence of cnts. the morphology of ag/cnts was characterized using x-ray diffraction and transmission electron microscopy (tem) techniques. the nylon-6 powder and 1 wt% ag/cnts mixture was dispersed uniformly using a noncontact spinning technique. the dried mixture was melted in a single screw extrusion machine and then extruded through an ori ... | 2010 | 20139493 |
| structural and biochemical characterization of srca, a multi-cargo type iii secretion chaperone in salmonella required for pathogenic association with a host. | many gram-negative bacteria colonize and exploit host niches using a protein apparatus called a type iii secretion system (t3ss) that translocates bacterial effector proteins into host cells where their functions are essential for pathogenesis. a suite of t3ss-associated chaperone proteins bind cargo in the bacterial cytosol, establishing protein interaction networks needed for effector translocation into host cells. in salmonella enterica serovar typhimurium, a t3ss encoded in a large genomic i ... | 2010 | 20140193 |
| chicken meat is an infection source of salmonella serovar infantis for humans in japan. | the study sets out to either confirm or refute a recent study's findings that chicken meat is an unlikely source of salmonella enterica subspecies enterica serovar infantis (salmonella infantis) in humans in the kyushu-okinawa region, japan. | 2010 | 20141347 |
| antibacterial activity of carvacrol and 2-nitro-1-propanol against single and mixed populations of foodborne pathogenic bacteria in corn flour dough. | cereal doughs are an important part of human diet, but at the same time can act as vehicles for the transmission of human pathogenic bacteria. in the present study, four pathogenic or toxinogenic bacteria (escherichia coli o157:h7, salmonella enterica serovar enteritidis, bacillus cereus and staphylococcus aureus) were inoculated in a dough made from corn flour in combination with the single antimicrobial compounds carvacrol and 2-nitro-1-propanol (2npoh). survival of single and mixed population ... | 2010 | 20141946 |
| so similar, yet so different: uncovering distinctive features in the genomes of salmonella enterica serovars typhimurium and typhi. | salmonella enterica represents a major human and animal pathogen. many s. enterica genomes have been completed and many more genome sequencing projects are underway, constituting an excellent resource for comparative genome analysis studies leading to a better understanding of bacterial evolution and pathogenesis. salmonella enterica serovar typhimurium and typhi are the best-characterized serovars, with the first being involved in localized gastroenteritis in many hosts and the latter causing a ... | 2010 | 20146749 |
| salmonella enterica serovar typhimurium vaccine strains expressing a nontoxic shiga-like toxin 2 derivative induce partial protective immunity to the toxin expressed by enterohemorrhagic escherichia coli. | shiga-like toxin 2 (stx2)-producing enterohemorrhagic escherichia coli (referred to as ehec or stec) strains are the primary etiologic agents of hemolytic-uremic syndrome (hus), which leads to renal failure and high mortality rates. expression of stx2 is the most relevant virulence-associated factor of ehec strains, and toxin neutralization by antigen-specific serum antibodies represents the main target for both preventive and therapeutic anti-hus approaches. in the present report, we describe t ... | 2010 | 20147499 |
| elucidation of the outer membrane proteome of salmonella enterica serovar typhimurium utilising a lipid-based protein immobilization technique. | salmonella enterica serovar typhimurium (s. typhimurium) is a major cause of human gastroenteritis worldwide. the outer membrane proteins expressed by s. typhimurium mediate the process of adhesion and internalisation within the intestinal epithelium of the host thus influencing the progression of disease. since the outer membrane proteins are surface-exposed, they provide attractive targets for the development of improved antimicrobial agents and vaccines. various techniques have been developed ... | 2010 | 20149234 |
| characterization of antimicrobial resistance, molecular and phage types of salmonella enterica serovar typhi isolations. | isolation rates in canada of salmonella enterica serovar typhi increased from 0.29 to 0.55 isolations/100,000 population during 2000-2006. although no ciprofloxacin resistance was detected, nalidixic acid resistance increased from 41% to 80%. multidrug-resistant s. typhi represented 18% of the strains tested. pulsed-field gel electrophoresis (pfge) analysis of 222 isolates resulted in 91 distinct patterns clustering into four major genetic similarity groups. the five most frequently occurring pf ... | 2010 | 20149265 |
| further evidence of plasmid-encoded bla-(ctx-m-15) and bla-(tem-1) genes in lebanese isolates of salmonella enterica serovar typhimurium that produce extended-spectrum beta-lactamase. | | 2010 | 20149296 |
| ribosomes lacking protein s20 are defective in mrna binding and subunit association. | the functional significance of ribosomal proteins is still relatively unclear. here, we examined the role of small subunit protein s20 in translation using both in vivo and in vitro techniques. by means of lambda red recombineering, the rpst gene, encoding s20, was removed from the chromosome of salmonella enterica var. typhimurium lt2 to produce a deltas20 strain that grew markedly slower than the wild type while maintaining a wild-type rate of peptide elongation. removal of s20 conferred a sig ... | 2010 | 20149799 |
| characterisation of pathogenic bacteria in a uasb-polishing pond system using molecular techniques. | molecular techniques have been commonly used to detect and quantify pathogenic bacteria in food, clinical and environmental samples, but in wastewater treatment plants few studies have been carried out. this work applied pcr with a specific set of primers to investigate pathogenic bacteria in a wastewater plant comprised of a uasb reactor followed by polishing ponds. in addition, in-situ hybridisation technique (fish) was used to estimate the abundance of escherichia coli in the system. accordin ... | 2010 | 20150719 |
| molecular basis for endotoxin neutralization by amphipathic peptides derived from the alpha-helical cationic core-region of nk-lysin. | an analysis of the interaction of the nk-lysin derived peptide nk-2 and of analogs thereof with bacterial lipopolysaccharide (lps, endotoxin) was performed to determine the most important biophysical parameters for an effective lps neutralization. we used microcalorimetry, ftir spectroscopy, zeta potential measurements, and small-angle x-ray scattering to analyze the peptide:lps binding enthalpy, the accessible lps surface charge, the fluidity of the lps hydrocarbon chains, their phase transitio ... | 2010 | 20153101 |
| protecting against antimicrobial effectors in the phagosome allows sodcii to contribute to virulence in salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium replicates in macrophages, where it is subjected to antimicrobial substances, including superoxide, antimicrobial peptides, and proteases. the bacterium produces two periplasmic superoxide dismutases, sodci and sodcii. although both are expressed during infection, only sodci contributes to virulence in the mouse by combating phagocytic superoxide. the differential contribution to virulence is at least partially due to inherent differences in the sodci and ... | 2010 | 20154132 |
| the second extracellular loop of pore-forming subunits of atp-binding cassette transporters for basic amino acids plays a crucial role in interaction with the cognate solute binding protein(s). | in the thermophile geobacillus stearothermophilus, the uptake of basic amino acids is mediated by an abc transporter composed of the substrate binding protein (receptor) artj and a homodimer each of the pore-forming subunit, artm, and the nucleotide-binding subunit, artp. we recently identified two putative binding sites in artj that might interact with the art(mp)(2) complex, thereby initiating the transport cycle (a. vahedi-faridi et al., j. mol. biol. 375:448-459, 2008). here we investigated ... | 2010 | 20154136 |
| the occurrence and sources of campylobacter spp., salmonella enterica and escherichia coli o157:h7 in the salmon river, british columbia, canada. | in this study, we wished to assess the prevalence and determine the sources of three zoonotic bacterial pathogens (salmonella, campylobacter, and escherichia coli o157:h7) in the salmon river watershed in southwestern british columbia. surface water, sewage, and animal faecal samples were collected from the watershed. selective bacterial culture and pcr techniques were used to isolate these three pathogens and indicator bacteria from these samples and characterize them. campylobacter was the mos ... | 2010 | 20154400 |
| eric pcr and rapd based fingerprinting of salmonella typhi strains isolated over a period of two decades. | salmonella enterica serotype typhi strains (n=113) were isolated from typhoid patients over a period of 2 decades, i.e. 1987-2006. rapd and eric pcr methods were used for random whole genome typing of these strains. eric pcr was found to be very efficient with the discriminatory index (di) of 0.9821 with 100% reproducibility. rapd was satisfactory in discriminating the strains (di=0.8978) but with poor reproducibility (40%). however, composite genotypic analysis was still better with di of 0.998 ... | 2010 | 20156601 |
| virulence mechanisms displayed by salmonella to impair dendritic cell function. | dendritic cells (dcs) link innate and adaptive immunity by directly recognizing pathogen-associated molecular patterns (pamps) on bacteria. dcs can capture and degrade bacteria and present their antigens on mhc molecules to t cells. pamp recognition promotes dc maturation, a phenotypic change that empowers them to prime naïve t cells. as a result, an adaptive immune response that specifically targets bacteria-derived antigens is initiated. consequently, any impairment of dc function might contri ... | 2010 | 20158475 |
| comparative study of leaf and stem bark extracts of parkia biglobosa against enterobacteria. | hydroethanolic and aqueous extracts of leaf and stem bark of parkia biglobosa (jacq) benth. (mimosaceae) were tested against clinical isolates escherichia coli, salmonella typhi, shigella dysenteriae and enterococcus faecalis, and corresponding collection strains e. coli cip 105 182, salmonella enterica cip 105 150, shigella dysenteriae cip 54-51 and enterococcus faecalis cip 103 907. discs of gentamicin, a broad spectrum antibiotic were used as positive controls. the results showed that all the ... | 2008 | 20161943 |
| evaluation of pet-related management factors and the risk of salmonella spp. carriage in pet dogs from volunteer households in ontario (2005-2006). | the purpose of this study was to determine pet-related management factors that may be associated with the presence of salmonella spp. in feces of pet dogs from volunteer households. from october 2005 until may 2006, 138 dogs from 84 households in ontario were recruited to participate in a cross-sectional study. five consecutive daily fecal samples were collected from each dog and enrichment culture for salmonella spp. was performed. a higher than expected number of the dogs (23.2%; 32/138) had a ... | 2011 | 20163574 |
| development of a multiplex primer extension assay for rapid detection of salmonella isolates of diverse serotypes. | food-borne salmonellosis is a major manifestation of gastrointestinal disease in humans across the globe. accurate and rapid identification methods could positively impact the identification of isolates, enhance outbreak investigation, and aid infection control. the snapshot multiplex system is a primer extension-based method that enables multiplexing of single nucleotide polymorphisms (snps). here the method has been developed for the identification of five salmonella serotypes, commonly detect ... | 2010 | 20164272 |
| 1-2 test. | a study was conducted to determine the efficacy of detecting low contamination levels of salmonella in peanut butter using the 1-2 test. this study was conducted under the aoac research institute emergency response validation program. a set of samples was analyzed by the 1-2 test and the u.s. food and drug administration's bacteriological analytical manual reference method. among the 90 total samples and controls, 32 samples were positive by the 1-2 test and the reference method. statistical ana ... | 2009 | 20166605 |
| transia plate salmonella gold. performance tested method 010602. | a study was conducted to determine the efficacy of detecting low contamination levels of salmonella in peanut butter using transia plate salmonella gold (tpsg). this study was conducted under the aoac emergency response validation program. a set of samples was each analyzed by the tpsg method and the u.s. food and drug administration's bacteriological analytical manual reference method. among the 45 total samples and controls, 26 were positive by the tpsg and 24 were positive by the reference me ... | 2009 | 20166607 |
| iq-check salmonella ii: real-time polymerase chain reaction test kit. performance tested method 010803. | iq-check salmonella ii is a real-time pcr kit for detection of salmonella in foods. specific oligonucleotide probes are used to detect target dna during the amplification, by hybridizing to the amplicons. these probes are linked to a fluorophore, which fluoresces only when hybridized to the target sequence. as part of an emergency response validation due to a massive outbreak and subsequent recall, peanut butter was tested to compare the performance of iq-check salmonella ii to the u.s. food and ... | 2009 | 20166609 |
| rapid'salmonella chromogenic medium. performance tested method 050701. | rapid'salmonella is a chromogenic medium for isolation and detection of salmonella spp. in food, based on two enzymatic activities. all presumptive salmonella-positive colonies are magenta, including lactose-positive salmonella. s. typhi, and s. paratyphi serotypes, due to detection of c8 esterase activity. in order to differentiate salmonella from other enterobacteriaceae, the medium includes a second chromogenic substrate. as part of an emergency response validation due to a massive outbreak a ... | 2009 | 20166610 |
| foodproof salmonella detection kit. performance tested method 120301. | the foodproof salmonella detection kit was previously validated in the performance tested methods program for the detection of salmonella species in a variety of foods, including milk powder, egg powder, coconut, cocoa powder, chicken breast, minced meat, sliced sausage, sausage, smoked fish, pasta, white pepper, cumin, dough, wet pet food, dry pet food, ice cream, watermelon, sliced cabbage, food dye, and milk chocolate. the method was shown to be equivalent to the u.s. food and drug administra ... | 2009 | 20166611 |
| singlepath salmonella. performance tested method 060401. | singlepath salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of salmonella spp. in food. a previous aoac performance tested method study evaluated singlepath salmonella as an effective method for the detection of salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. in this emergency response validation extension ... | 2009 | 20166612 |
| taqman salmonella enterica detection kit. performance tested method 020803. | peanut butter spiked with salmonella enterica ser. typhimurium was prepared by an independent laboratory and sent to applied biosystems to determine the sensitivity and specificity of the taqman salmonella enterica detection kit for detecting salmonella in peanut butter. the samples were spiked at three levels: five no-spike (0 cfu/25 g); 20 low-spike (0.2 cfu/25 g); and 20 high-spike (2 cfu/25 g). they were coded to create a blind set of 45 samples. the samples were processed based on an unpair ... | 2009 | 20166614 |
| dupont qualicon bax system polymerase chain reaction assay. performance tested method 100201. | a recent outbreak of salmonella in peanut butter has highlighted the need for validation of rapid detection methods. a multilaboratory study for detecting salmonella in peanut butter was conducted as part of the aoac research institute emergency response validation program for methods that detect outbreak threats to food safety. three sites tested spiked samples from the same master mix according to the u.s. food and drug administration's bacteriological analytical manual (fda-bam) method and th ... | 2009 | 20166615 |
| chromagar salmonella detection test kit. performance tested method 020502. | bbl chromagar salmonella was evaluated by an external food testing laboratory for the recovery of salmonella in peanut butter using the u.s. food and drug administration's bacteriological analytical manual (fda-bam) procedure. the peanut butter was found to be negative for the presence of salmonella and, therefore, was seeded with heat-stressed salmonella at target concentrations of 0.2 and 2 cfu/25 g. the salmonella-seeded samples remained at room temperature for 14 days before analysis to stab ... | 2009 | 20166616 |
| attenuated salmonella choleraesuis-mediated rnai targeted to conserved regions against foot-and-mouth disease virus in guinea pigs and swine. | in this study, specific sequences within three genes (3d, vp4 and 2b) of the foot-and-mouth disease virus (fmdv) genome were determined to be effective rnai targets. these sequences are highly conserved among different serotype viruses based on sequence analysis. small interfering rna (sirna)-expressing plasmids (p3d-nt19, p3d-nt56, pvp4-nt19, pvp4-nt65 and p2b-nt25) were constructed to express sirna targeting 3d, vp4 and 2b, respectively. the antiviral potential of these sirna for various fmdv ... | 2010 | 20167192 |
| cell biology. rise of the rival. | | 2010 | 20167774 |
| [antimicrobial susceptibility and resistance genes among salmonella enterica serovar weltevreden isolates from humans, farm animals, and the environment in okinawa prefecture between 1992 and 2007]. | between 1992 and 2007, a total of 86 isolates of salmonella enterica weltevreden were obtained from clinical human samples (n = 41), 45 farm animals and their environment on 20 farms, including poultry (n = 25), beef cattle (n = 5), swine (n = 5), dairy cattle (n = 3), mice (n = 2), pony (n = 1), fly (n = 1) and feed samples (n = 3), in okinawa prefecture, japan. only seven isolates (8.1%) of the isolates were resistance to one or more antimicrobial agents tested; six streptomycin (7.0%), six ox ... | 2010 | 20170010 |
| [a 14-year-old healthy boy with splenic abscess due to salmonella enterica serovar senftenberg]. | salmonella enterica serovar senftenberg may very rarely cause splenic abscess, which can be diagnosed using gallium scintigraphy and drained. a 14-year-old boy admitted for stomachache, diarrhea and fever and diagnosed from his symptoms as having enteritis did not respond when treated with fosfomycin, meropenem, and clindamycin. a low-density splenic area seen in abdominal computed tomography on admission did not show contrast medium enhancement. gallium scintigraphy on hospital day 10, however, ... | 2010 | 20170018 |
| immunogenic properties of a recombinant fusion protein containing the c-terminal 19 kda of plasmodium falciparum merozoite surface protein-1 and the innate immunity agonist flic flagellin of salmonella typhimurium. | in a recent study, we demonstrated the immunogenic properties of a new malaria vaccine polypeptide based on a 19 kda c-terminal fragment of the merozoite surface protein-1 (msp1(19)) from plasmodium vivax and an innate immunity agonist, the salmonella enterica serovar typhimurium flagellin (flic). herein, we tested whether the same strategy, based on the msp1(19) component of the deadly malaria parasite plasmodium falciparum, could also generate a fusion polypeptide with enhanced immunogenicity. ... | 2010 | 20170765 |
| spontaneous excision of the salmonella enterica serovar enteritidis-specific defective prophage-like element phise14. | salmonella enterica serovar enteritidis has emerged as a major health problem worldwide in the last few decades. dna loci unique to s. enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. an in silico comparison of 16 salmonella genomic sequences revealed the presence of an approximately 12.5-kb genomic island (gei) specific to the sequenced s. enteritidis strain nctc13349. the gei is inserted at the 5' end of gene ydao ( ... | 2010 | 20172996 |
| autonomous growth of isolated single listeria monocytogenes and salmonella enterica serovar typhimurium cells in the absence of growth factors and intercellular contact. | the aim of this study was to observe growth of isolated single bacterial cells in the absence of growth factors and intercellular contact. in order to exclude stochastic uncertainties induced by dilution series, a new micromanipulation method was developed to ensure explicit results under visual control. this was performed with particular care for production of single prokaryotic cells and subsequent investigation of their autonomous growth. over 450 single isolated listeria monocytogenes and sa ... | 2010 | 20173058 |
| an antimicrobial peptide that targets dna repair intermediates in vitro inhibits salmonella growth within murine macrophages. | the hexapeptide wrwycr was previously identified on the basis of its ability to inhibit bacteriophage lambda integrase-mediated recombination by trapping and preventing resolution of the holliday junction intermediate. this peptide inhibits several unrelated dna repair enzymes that bind to and process holliday junctions and branched dna substrates. wrwycr and its d stereoisomer, wrwycr, are bactericidal against both gram-positive and gram-negative bacteria, causing the accumulation of dna breaks ... | 2010 | 20176906 |
| gallstones play a significant role in salmonella spp. gallbladder colonization and carriage. | salmonella enterica serovar typhi can colonize the gallbladder and persist in an asymptomatic carrier state that is frequently associated with the presence of gallstones. we have shown that salmonellae form bile-mediated biofilms on human gallstones and cholesterol-coated surfaces in vitro. here, we test the hypothesis that biofilms on cholesterol gallbladder stones facilitate typhoid carriage in mice and men. naturally resistant (nramp1(+/+)) mice fed a lithogenic diet developed cholesterol gal ... | 2010 | 20176950 |
| amplification of the gene for isoleucyl-trna synthetase facilitates adaptation to the fitness cost of mupirocin resistance in salmonella enterica. | mutations that cause resistance to antibiotics in bacteria often reduce growth rate by impairing some essential cellular function. this growth impairment is expected to counterselect resistant organisms from natural populations following discontinuation of antibiotic therapy. unfortunately (for disease control) bacteria adapt and improve their growth rate, often without losing antibiotic resistance. this adaptation process was studied in mupirocin-resistant (mup(r)) strains of salmonella enteric ... | 2010 | 20176977 |
| proton-conductivity assay of plugged and unplugged mota/b proton channel by cytoplasmic phluorin expressed in salmonella. | mota and motb form the proton-channel complex of the proton-driven bacterial flagellar motor. a plug segment of escherichia coli motb suppresses proton leakage through the mota/b complex when it is not assembled into the motor. using a ratiometric ph indicator protein, phluorin, we show that the proton-conductivity of a salmonella mota/b complex not incorporated into the motor is two orders of magnitude lower than that of a complex that is incorporated and activated. this leakage is, however, si ... | 2010 | 20178785 |
| pulsed-field gel electrophoresis diversity of human and bovine clinical salmonella isolates. | pulsed-field gel electrophoresis (pfge) characterization of 335 temporally and spatially matched clinical, bovine, and human salmonella enterica subsp. enterica isolates revealed 167 xbai pfge patterns. these isolates were previously classified into 51 serotypes and 73 sequence types, as determined by multilocus sequence typing. discriminatory power of pfge (simpson's index, d = 0.991) was considerably higher than that of multilocus sequence typing (d = 0.920) or serotyping (d = 0.913). although ... | 2010 | 20180633 |
| sialic acid is required for nonspecific adherence of salmonella enterica ssp. enterica serovar typhi on caco-2 cells. | to initiate infection, bacteria must adhere to and colonize host tissues. specific and nonspecific mechanisms participate in the adherence process. salmonella enterica ssp. enterica serovar typhi (s. typhi) must first adhere to the intestinal epithelium to invade and disseminate throughout the host. in this study, the role of colonic epithelial cell surface sialic acid in the adherence of s. typhi was defined. neuraminidase treatment of colonic caco-2 cells removed 27-58% of surface sialic acid. ... | 2010 | 20180850 |
| relatedness of the o-polysaccharide structures of escherichia coli o123 and salmonella enterica o58, both containing 4,6-dideoxy-4-{n-[(s)-3-hydroxybutanoyl]-d-alanyl}amino-d-glucose; revision of the e. coli o123 o-polysaccharide structure. | o-polysaccharides were isolated by mild acid degradation of the lipopolysaccharides of escherichia coli o123 and salmonella enterica o58 and studied by chemical methods and 2d (1)h and (13)c nmr spectroscopy, including experiments in a h(2)o/d(2)o mixture, which enabled observation of correlations for nitrogen-linked protons. the following structure of the o-polysaccharide of e. coli o123 was established: -->3)-beta-d-quip4nalahb-(1-->6)-alpha-d-glcpnac-(1-->3)-alpha-l-quipnac-(1-->3)-alpha-d-gl ... | 2010 | 20181329 |
| salmonella pathogenicity island 1 (spi-1) type iii secretion of sopd involves n- and c-terminal signals and direct binding to the invc atpase. | salmonella enterica serovar typhimurium (s. typhimurium) is an important pathogen and a causative agent of gastroenteritis. during infection, s. typhimurium assembles molecular-needle complexes termed type iii secretion (t3s) systems to translocate effector proteins from the bacterial cytoplasm directly into the host cell. the t3s signals that direct the secretion of effectors still remain enigmatic. sopd is a key t3s effector contributing to the systemic virulence of s. typhimurium and the deve ... | 2010 | 20185511 |
| salmonella enterica serotype cerro among dairy cattle in new york: an emerging pathogen? | the focus of this study was salmonella enterica serotype cerro, a potentially emerging pathogen of cattle. our objectives were to document the within-herd prevalence of salmonella cerro among a sample of new york dairy herds, to describe the antimicrobial resistance patterns and pulsed-field gel electrophoresis types of the isolates, and to elucidate the status of this serotype as a bovine pathogen. data were collected prospectively from dairy herds throughout new york that had at least 150 lact ... | 2010 | 20187753 |
| sub-iliac lymph nodes at slaughter lack ability to predict salmonella enterica prevalence for swine farms. | the aim of this study was to assess the value of deep systemic sub-iliac lymph nodes collected at slaughter as predictors of salmonella prevalence in live hogs. an observational study was conducted on 24 farms from september 2006 to february 2009. at least one cohort of market-weight pigs was visited for each farm. within each cohort, 30 farm fecal samples on farm and 30 sub-iliac lymph nodes from matched pigs at slaughter were collected. samples were cultured for salmonella enterica and serotyp ... | 2010 | 20187754 |
| drug resistance patterns in salmonella enterica subspecies enterica serotype typhi strains isolated over a period of two decades, with special reference to ciprofloxacin and ceftriaxone. | fluoroquinolone-resistant salmonella enterica subspecies enterica serotype typhi are being increasingly reported from the asian subcontinent. this has been hypothesised to be due to a double mutation in the gyra gene. a total of 113 s. typhi strains isolated during 1987-2006 in a tertiary-level hospital of north india were monitored for their antibiotic susceptibility by the disk diffusion and minimum inhibitory concentration (mic) methods. the study period was arbitrarily divided into four equa ... | 2010 | 20188522 |
| [study on the epidemiological characteristics and molecular typing of salmonella enterica subsp. enterica serovar senftenberg in shanghai]. | to study the molecular epidemiological characteristics of salmonella enterica subsp. enterica serovar senftenberg (salmonella senftenberg) in shanghai, from 2006 to 2007. | 2009 | 20193231 |
| anti-infective mechanisms induced by a probiotic lactobacillus strain against salmonella enterica serovar typhimurium infection. | the prevention of pathogen infections is one of the most extensively studied effects of probiotics. l. casei crl 431 is a probiotic bacterium and its effects on the gut immune cells have been extensively studied. the aim of the present study was to determine, using a mouse model, the preventive and therapeutic effect of l. casei crl 431 to achieve protection against salmonella enteritidis serovar typhimurium infection. in both previous and continuous (previous and post-infection) probiotic admin ... | 2010 | 20193971 |
| salmonella enterica serovar typhimurium-induced placental inflammation and not bacterial burden correlates with pathology and fatal maternal disease. | food-borne infections caused by salmonella enterica species are increasing globally, and pregnancy poses a high risk. pregnant mice rapidly succumb to s. enterica serovar typhimurium infection. to determine the mechanisms involved, we addressed the role of inflammation and bacterial burden in causing placental and systemic disease. in vitro, choriocarcinoma cells were a highly conducive niche for intracellular s. typhimurium proliferation. while infection of mice with s. typhimurium wild-type (w ... | 2010 | 20194592 |
| chronic murine typhoid fever is a natural model of secondary hemophagocytic lymphohistiocytosis. | hemophagocytic lymphohistiocytosis (hlh) is a hyper-inflammatory clinical syndrome associated with neoplastic disorders especially lymphoma, autoimmune conditions, and infectious agents including bacteria, viruses, protozoa and fungi. in both human and veterinary medicine, hemophagocytic histiocytic disorders are clinically important and frequently fatal. hlh in humans can be a primary (familial, autosomal recessive) or secondary (acquired) condition, with both types generally precipitated by an ... | 2010 | 20195482 |
| structure of the o-polysaccharide of salmonella enterica o41. | an o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of salmonella enterica o41, and the following structure of the o-unit was determined by chemical analyses along with 1d and 2d (1)h and (13)c nmr spectroscopy: -->2)-beta-d-manp-(1-->4)-alpha-d-glcp-(1-->3)-alpha-l-quipnac-(1-->3)-alpha-d-glcpnac-(1--> where quinac stands for 2-acetamido-2,6-dideoxyglucose. the structure established is in agreement with the o-antigen gene cluster of s. enterica o41 and tentative ... | 2010 | 20199769 |
| salmonella pathogenicity island 1 differentially modulates bacterial entry to dendritic and non-phagocytic cells. | salmonella enterica serovar typhimurium can enter non-phagocytic cells, such as intestinal epithelial cells, by virtue of a type three secretion system (ttss) encoded in the salmonella pathogenicity island 1 (spi-1), which translocates bacterial effector molecules into the host cell. salmonella can also be taken up by dendritic cells (dcs). although the role of spi-1 in non-phagocytic cell invasion is well established, its contribution to invasion of phagocytic cells has not been evaluated. here ... | 2010 | 20201987 |
| probiotics down-regulate genes in salmonella enterica serovar typhimurium pathogenicity islands 1 and 2. | salmonella typhimurium pathogenesis relies mainly on the expression of genes of two pathogenicity islands, salmonella pathogenicity islands 1 and 2 (spi1 and spi2). each island has its own pattern of expression and regulation. success in suppression of the responsible key activator of each island would be an effective way of controlling salmonella, especially with the emerging problem of antibiotic-resistant strains. probiotics have been shown to inhibit several foodborne pathogens, and their mo ... | 2010 | 20202329 |
| lessons learned from the management of a national outbreak of salmonella ohio linked to pork meat processing and distribution. | during the summer of 2005, an increase in reports of human cases of salmonella enterica serovar ohio infection was observed in belgium. during 11 weeks, between 1 july and 13 september, 60 cases of laboratory-confirmed salmonella ohio infection were reported to the national reference centre for salmonella, with a peak onset of symptoms in the third week of july. all clinical isolates caused self-limiting gastroenteritis; both genders (32 males and 28 females) and all age groups (three children < ... | 2010 | 20202340 |
| synergistic effects of ethanol and uv radiation to reduce levels of selected foodborne pathogenic bacteria. | the purpose of this study was to determine whether combined treatments would produce synergistic disinfection effects on food products during food processing compared with single treatments. we investigated the bactericidal effects of a commercial chemical disinfectant (ethanol) and of uv radiation on bacillus cereus f4810/72, cronobacter sakazakii kctc 2949, staphylococcus aureus atcc 35556, escherichia coli atcc 10536, and salmonella enterica typhimurium no/na in vitro. various concentrations ... | 2010 | 20202345 |
| salmonella isolated from the feces of migrating cranes at the izumi plain (2002-2008): serotype, antibiotic sensitivity and pfge type. | from november 2002 to february 2008, 2,251 crane feces were collected at the izumi plain in kagoshima prefecture. salmonella enterica was isolated from 359 feces (15.9%), of which 332 (92.5%) were salmonella typhimurium (st), 9 were s. hvittingfoss/ii, 4 were s. abaetetuba, 3 were s. enteritidis, 2 were s. konstanz, 1 was s. pakistan and 8 were untyped isolates, respectively. against 12 antimicrobial agents, no resistant strains were found in 154 isolates examined, but one was found to be resist ... | 2010 | 20203434 |
| synergistic antimicrobial effect of nisin and p-cymene on salmonella enterica serovar typhi in vitro and on ready-to-eat food. | foods contaminated with salmonella enterica serovar typhi are a major cause of typhoid fever, leading to public health problems and economic losses worldwide. nisin and rho-cymene were tested in this study for their antimicrobial activity against s. typhi at 4 degrees c and 37 degrees c. nisin and rho-cymene, when used separately, did not inhibit the bacterium at either temperature. a synergistic antimicrobial effect between both compounds was observed when they were used simultaneously. this sy ... | 2010 | 20208367 |
| the c terminus of sipc binds and bundles f-actin to promote salmonella invasion. | salmonella enterica serovar typhimurium invade non-phagocytic cells by injecting bacterial effector proteins to exploit the host actin cytoskeleton network. sipc is such a salmonella effector known to nucleate actin, bundle f-actin, and translocate type iii effectors. the molecular mechanism of how sipc bundles f-actin and sipc domains responsible for these activities are not well characterized. we successfully separated these activities through a series of genetic deletion/insertions in sipc. w ... | 2010 | 20212042 |
| the tandem inversion duplication in salmonella enterica: selection drives unstable precursors to final mutation types. | during growth under selection, mutant types appear that are rare in unselected populations. stress-induced mechanisms may cause these structures or selection may favor a series of standard events that modify common preexisting structures. one such mutation is the short junction (sj) duplication with long repeats separated by short sequence elements: ab*(cd)*(cd)*e (* = a few bases). another mutation type, described here, is the tandem inversion duplication (tid), where two copies of a parent seq ... | 2010 | 20215473 |
| differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and tzm-bl cells by endotoxin-mediated chemokine and gamma interferon production. | bacterial lipopolysaccharide (endotoxin) is a frequent contaminant of biological specimens and is also known to be a potent inducer of beta-chemokines and other soluble factors that inhibit hiv-1 infection in vitro. though lipopolysaccharide (lps) has been shown to stimulate the production of soluble hiv-1 inhibitors in cultures of monocyte-derived macrophages, the ability of lps to induce similar inhibitors in other cell types is poorly characterized. here we show that lps exhibits potent anti- ... | 2010 | 20218881 |
| ppgpp-mediated stationary phase induction of the genes encoded by horizontally acquired pathogenicity islands and cob/pdu locus in salmonella enterica serovar typhimurium. | salmonella enterica is highly diverse in terms of genome structure, which is at least partly due to the horizontal transfer of genetic elements from various sources. in this study, we examined the expression profiles of such genes in salmonella pathogenicity islands (spis) and the cob/pdu locus, horizontally acquired large dna segments, during growth under standard growth conditions. transcripts from exponentially growing and early stationary phase salmonellae were compared using various methods ... | 2010 | 20221735 |
| influence of 5 major salmonella pathogenicity islands on nk cell depletion in mice infected with salmonella enterica serovar enteritidis. | in this study we were interested in the colonisation and early immune response of balb/c mice to infection with salmonella enteritidis and isogenic pathogenicity island free mutants. | 2010 | 20226037 |
| differences in attachment of salmonella enterica serovars to cabbage and lettuce leaves. | this study investigated the ability of five salmonella enterica serovars to attach to and colonize intact and cut lettuce (iceberg, romaine) and cabbage surfaces. biofilm formation and attachment of salmonella serovars to intact and cut leaves were determined. populations of loosely and strongly attached salmonella were obtained to calculate the attachment strength (s(r)). biofilm formation, as determined by microtiter plate assay, varied with strain and growth medium used. salmonella tennessee ... | 2010 | 20226552 |
| inactivation of salmonella enteritidis strains by combination of high hydrostatic pressure and nisin. | the effects of high hydrostatic pressure (hhp) and nisin treatment alone and in combination on cellular components and viability of two salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) strains were evaluated by differential scanning calorimetry (dsc) and plate counting in order to evaluate the relative resistance and optimize the treatment conditions. s. enteritidis fda and osu 799 strains were subjected to hhp (0.1-550 mpa for 10 min at 25 degrees c) alone and in combina ... | 2010 | 20226554 |
| in vitro antagonistic activities of animal intestinal strains against swine-associated pathogens. | a wide range of enteropathogens cause costly diarrhoeal diseases in fattening piglets and account for food-related infections in humans. the objective of this study was to screen beneficial bacterial strains from the gastrointestinal tract of various animal sources for antagonistic activity against diverse pathogens associated with hazardous pig production times. using agar spot assays, 15 well-characterized strains belonging to lactobacillus, enterococcus, bifidobacterium and bacillus were stud ... | 2010 | 20226602 |
| the salmonella enterica serovar typhimurium qseb response regulator negatively regulates bacterial motility and swine colonization in the absence of the qsec sensor kinase. | salmonella enterica serovar typhimurium (s. typhimurium) responds to the catecholamine, norepinephrine by increasing bacterial growth and enhancing motility. in this study, iron with or without the siderophore, ferrioxamine e also enhanced bacterial motility. iron-enhanced motility was growth-rate dependent, while norepinephrine-enhanced motility was growth-rate independent. the outer membrane catecholate receptors, iron, fepa and cira (required for norepinephrine-enhanced growth) were not requi ... | 2010 | 20227482 |
| kinetics of growth and inactivation of salmonella enterica serotype typhimurium dt104 in pasteurised liquid egg products. | the potential impact of post-pasteurisation contamination of liquid egg products with the multi-antibiotic resistant pathogen salmonella enterica serotype typhimurium definitive type 104 (dt104) was assessed by determining the viability of this bacterium in whole egg, albumen and 10% w/w sugared and salted yolk incubated at 4-42 degrees c. results indicated that populations of s. typhimurium dt104 were slowly inactivated in all four products when stored at 4 degrees c. however, based on the typi ... | 2010 | 20227605 |
| cryptdin-2: a novel therapeutic agent for experimental salmonella typhimurium infection. | salmonella infections represent a major health hazard and have been responsible for a number of epidemics. in view of the emergence of multidrug-resistant salmonella strains, there is a need for therapeutic alternatives. the purpose of this study was to evaluate the therapeutic potential of cryptdin-2 (a paneth cell antimicrobial peptide) against salmonella infection. | 2010 | 20228082 |
| accuracy and sensitivity of commercial pcr-based methods for detection of salmonella enterica in feed. | the present study compared the performance of commercial pcr-based salmonella enterica detection methods (bax system q7, the iq-check salmonella ii kit, and the taqman salmonella enterica detection kit) with culture-based methods (modified semisolid rappaport-vassiliadis [msrv] and nmkl71) in spiked and naturally contaminated samples of feed mill scrapings (fms), palm kernel meal (pkm), pelleted feed (pf), rape seed meal (rsm), soybean meal (sm), and wheat grain (wg). when results from the vario ... | 2010 | 20228106 |
| imaging type-iii secretion reveals dynamics and spatial segregation of salmonella effectors. | the type-iii secretion system (t3ss) enables gram-negative bacteria to inject effector proteins into eukaryotic host cells. upon entry, t3ss effectors work cooperatively to reprogram host cells, enabling bacterial survival. progress in understanding when and where effectors localize in host cells has been hindered by a dearth of tools to study these proteins in the native cellular environment. we report a method to label and track t3ss effectors during infection using a split-gfp system. we demo ... | 2010 | 20228815 |
| the multi-copper-ion oxidase cueo of salmonella enterica serovar typhimurium is required for systemic virulence. | salmonella enterica serovar typhimurium possesses a multi-copper-ion oxidase (multicopper oxidase), cueo (also known as cuid), a periplasmic enzyme known to be required for resistance to copper ions. cueo from s. typhimurium was expressed as a recombinant protein in escherichia coli, and the purified protein exhibited a high cuprous oxidase activity. we have characterized an s. typhimurium cueo mutant and confirmed that it is more sensitive to copper ions. using a murine model of infection, it w ... | 2010 | 20231415 |
| identification by pcr of non-typhoidal salmonella enterica serovars associated with invasive infections among febrile patients in mali. | in sub-saharan africa, non-typhoidal salmonella (nts) are emerging as a prominent cause of invasive disease (bacteremia and focal infections such as meningitis) in infants and young children. importantly, including data from mali, three serovars, salmonella enterica serovar typhimurium, salmonella enteritidis and salmonella dublin, account for the majority of non-typhoidal salmonella isolated from these patients. | 2010 | 20231882 |
| periplasmic peptidyl-prolyl isomerases sura and fkpa play an important role in the starvation-stress response (ssr) of salmonella enterica serovar typhimurium. | carbon-energy source (c)-starved cells of salmonella enterica serovar typhimurium (s. typhimurium) are remarkably more resistant to stress than actively growing ones. carbon-starved s. typhimurium is capable of withstanding extended periods of starvation and assault from a number of different stresses that rapidly kill growing cells. these unique properties of the c-starved cell are the direct result of a series of genetic and physiological adaptations referred to as the starvation-stress respon ... | 2010 | 20232248 |
| structural and functional characterization of three dsba paralogues from salmonella enterica serovar typhimurium. | in prototypic escherichia coli k-12 the introduction of disulfide bonds into folding proteins is mediated by the dsb family of enzymes, primarily through the actions of the highly oxidizing protein ecdsba. homologues of the dsb catalysts are found in most bacteria. interestingly, pathogens have developed distinct dsb machineries that play a pivotal role in the biogenesis of virulence factors, hence contributing to their pathogenicity. salmonella enterica serovar (sv.) typhimurium encodes an exte ... | 2010 | 20233716 |
| intrafamilial transmission of extended-spectrum-beta-lactamase-producing escherichia coli and salmonella enterica babelsberg among the families of internationally adopted children. | international adoption from developing countries has become an increasing phenomenon in recent years. given the high prevalence of multidrug-resistant (mdr) bacteria in these countries, the adopted children represent a group at risk for both carriage and infection with mdr bacteria. the dynamics of intrafamilial transmission of mdr bacteria after adoption was studied in a prospective study from january 2002 to january 2005. | 2010 | 20233775 |
| mechanisms and physiological effects of protamine resistance in salmonella enterica serovar typhimurium lt2. | protamines are cationic peptides that exert antimicrobial activity. we have examined the evolution of bacterial resistance to protamine sulphate and the resulting effects on fitness and physiology, with the objective of increasing knowledge about mechanisms of bacterial resistance to antimicrobial peptides. | 2010 | 20233778 |
| salicylate reduces the antimicrobial activity of ciprofloxacin against extracellular salmonella enterica serovar typhimurium, but not against salmonella in macrophages. | salicylate, a potent inducer of the mara activator in salmonella enterica, is the principal metabolite of aspirin, which is often consumed for medicinal and cosmetic uses. our research was aimed at testing if salicylate activates the mar regulon in macrophage-associated salmonella (intracellular bacteria), and investigating its effects on bacterial susceptibility to ciprofloxacin extracellularly and intracellularly. | 2010 | 20237076 |
| coronary arteritis with fatal thrombosis due to salmonella choleraesuis variety kunzendorf. | | 1947 | 20262639 |
| sulphonamide investigations; treatment of experimental pasteurella septica, ery. rhusiopathiae and s. cholerae-suis infections in mice with sulphathiazole, sulphadiazine, sulphamezathine and sulphapyridine. | | 1947 | 20265764 |
| listeria and salmonella bacterial vectors of tumor-associated antigens for cancer immunotherapy. | this review covers the use of the facultative intracellular bacteria, listeriamonocytogenes and salmonella enterica serovar typhimurium as delivery systems for tumor-associated antigens in tumor immunotherapy. because of their ability to infect and survive in antigen presenting cells, these bacteria have been harnessed to deliver tumor antigens to the immune system both as bacterially expressed proteins and encoded on eukaryotic plasmids. they do this in the context of strong innate immunity, wh ... | 2010 | 20299242 |