| a high-resolution proton nuclear-magnetic-resonance investigation of carp hemoglobin. conformational differences between carp and human normal adult hemoglobins in solution. | the high-resolution proton nuclear magnetic resonance spectra of carp hemoglobin have been compared to those of human normal adult hemoglobin. carp deoxy and carbonmonoxy hemoglobins in the deoxy-type quaternary state exhibit two downfield exchangeable proton resonances as compared to four seen in human normal adult deoxyhemoglobin. this suggests that two of the hydrogen bonds present in human normal adult deoxyhemoglobin are absent or occur in very different environments in carp hemoglobin. one ... | 1984 | 6329750 |
| intracellular distribution of fumarase in various animals. | the subcellular distribution of fumarase was investigated in the liver of various animals and in several tissues of the rat. in the rat liver, fumarase was predominantly located in the cytosolic and mitochondrial fractions, but not in the peroxisomal fraction. the amount of fumarase associated with the microsomes was less than 5% of the total enzyme activity. the investigation of the intracellular distribution of hepatic fumarase of the rat, mouse, rabbit, dog, chicken, snake, frog, and carp rev ... | 1984 | 6333419 |
| separation of lymphocyte subpopulations in carp cyprinus carpio l. by monoclonal antibodies: immunohistochemical studies. | lymphoid cell populations in various organs of the carp cyprinus carpio l. were investigated using a series of mouse monoclonal antibodies raised against carp thymocytes and carp serum ig. clones have been designated as ig+t+, ig+t- or ig-t+ on the basis of the reactivity with thymocytes and serum ig in the enzyme-linked immunosorbent assay (elisa) screening. their reaction to the lymphoid organs of carp was investigated on cryostat sections and cytocentrifuge slides using immunoperoxidase techn ... | 1983 | 6336715 |
| identification of a heparin activated amidolytic enzyme in carp (cyprinus carpio) plasma. | a heparin activated amidolytic enzyme capable of cleaving synthetic thrombin sensitive chromogenic substrates was identified from the plasma of the carp (cyprinus carpio). activation was inhibited by kcl, protamine sulphate and human plasma. heparin was not required for the continued action of the enzyme. active enzyme was irreversibly inhibited by dfp. the ph and temperature optima was studied and the enzyme semi purified by sephadex g100 superfine and sephadex a50 chromatography. an approximat ... | 1983 | 6342205 |
| immunohistochemical demonstration of serotonin-containing csf-contacting neurons in the submammalian paraventricular organ. | the distribution and morphological aspects of the serotonin-containing neurons in the paraventricular organ of the carp, frog, turtle and chicken were studied by means of an immunoperoxidase technique using serotonin antiserum. in all species the serotonin-containing neurons were seen to have the appearance of the csf-contacting neurons and to be distributed in the pars ependymalis and the pars hypendymalis of the organ. particularly, in the frog, the serotonin-containing csf-contacting neurons, ... | 1983 | 6345483 |
| transport of proteins into mitochondria: a high conservation of precursor uptake and processing system. | ornithine transcarbamylase (ec 2.1.3.3) of rat (rattus norvegicus var. albus) liver, a urea cycle enzyme, is synthesized extramitochondrially as a larger precursor which is transported posttranslationally into mitochondria and processed to the mature enzyme. the precursor synthesized in vitro was taken up and processed to the mature enzyme by isolated pigeon (columba livia var. domestica) liver and frog (rana catesbeiana) liver mitochondria. carp (cyprinus carpio) liver mitochondria could also p ... | 1983 | 6347507 |
| high mutagenic potency of several polycyclic aromatic hydrocarbons induced by liver postmitochondrial fractions from control and xenobiotic-treated immature carp. | the metabolism of carcinogens in fish was examined by measuring the activation of different polycyclic aromatic hydrocarbons (pah) by carp (cyprinus carpio l.) liver post-mitochondrial fractions (s9) using the salmonella typhimurium ta100 reverse mutation assay. for this study, 1 non-carcinogen, anthracene (an), and 4 carcinogens, chrysene (chr), benzo[a]pyrene (bap), 3-methylcholanthrene (3mc) and 7,12-dimethylbenzanthracene (dmba), were chosen. the bioactivating potency of the metabolic system ... | 1983 | 6348528 |
| purification and properties of enolase from carp (cyprinus carpio). comparison with enolases from mammals' muscles and yeast. | the enolase (2-phospho-d-glycerate hydrolyase e.c. 4.2.1.11) from carp muscle was obtained, the specific activity--88 u/mg of protein. km for 2-phosphoglycerate was 0.313 mm and for phosphoenolpyruvate--0.76 mm. the enzyme is active only in the presence of divalent metal ions, mg2+ being the best activator. the phosphate and fluoride decreased the activity of enzyme. the molecular weight of the dimeric form of enzyme was found to be 93,000. the enzyme is immunologically different from pig muscle ... | 1983 | 6352164 |
| [quantitative determination of spring viremia carp virus (svcv) antigens using immunofluorescence and immunoperoxidase plaque technic]. | | 1983 | 6353812 |
| immunochemical analysis of cod fish allergen m: locations of the immunoglobulin binding sites as demonstrated by the native and synthetic peptides. | the major allergen of codfish (allergen m) is a muscle protein belonging to the family of calcium binding parvalbumins. the primary structure of the molecule was established and the molecular weight was estimated from the sequence data to be 12,328. allergen m consists of 113 amino acid residues and one residue of glucose. a molecular arrangement of three domains (ab, cd and ef--the latter two bind one ca2+ ion each) was described for allergen m, analogous to carp parvalbumin pi 4.25. the sugges ... | 1983 | 6356964 |
| carp preproinsulin cdna sequence and evolution of insulin genes. | the nucleic acid sequence of the preproinsulin cdna of carp (cyprinus carpio), cloned in the psti site of pbr322 ( liebscher et al. 1980), has been determined. the sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. the nucleotide sequence confirms the previously established amino acid sequence of carp insulin ( makower et al. 1982) and determines those of the s ... | 1984 | 6373397 |
| clonorchis sinensis: life cycle, intermediate hosts, transmission to man and geographical distribution in korea. | the snail host of clonorchis sinensis in korea is referred to as parafossarulus manchouricus, but it has been recorded also as bulimus striatulus japonicus in literature published up to late 1950. the specific name of the snail was corrected such that bulimus striatulus of china, japan, and korea should be incorporated in the species of p. manchouricus. in general, the snail exists in limited areas of the rivers han, kum, mankyung, naktong, and yeongsan, and the infestation of the snail with the ... | 1984 | 6391501 |
| [spore-forming bacteria in carp and rainbow trout, raised in waste waters from a steam power plant]. | | 1984 | 6400797 |
| rds-127 (2-di-n-propylamino-4,7-dimethoxyindane): central effects of a new dopamine receptor agonist. | apomorphine (apo), 2-di-n-propylamino-4,7-dimethoxyindane (rds-127) and 2-di-n-propylamino-5,8-dimethoxytetralin (jmc-181) were examined on a variety of biochemical and pharmacological assays to determine their possible interaction with dopamine (da) receptors. nanomolar concentrations of all three compounds displaced [3h]apo from specific high-affinity binding sites in rat striatal membrane preparations, while higher concentrations were required to displace [3h]spiperone or [3h]rauwolscine. apo ... | 1983 | 6401334 |
| comparative study of the glial fibrillary acidic protein in vertebrates by pap immunohistochemistry. | glial fibrillary acidic protein (gfa) has been visualized by direct peroxidase antiperoxidase (apa) immunohistochemistry in various vertebrates (cyclostomes, teleosts, amphibians, reptiles, birds, and several placental mammals). in this study gfa-immunoreactivity (gfa-i) was observed in all species examined except in cyclostomes and amphibians. two types of immunoreactive elements were observed: astrocytes and long processes without visible somata. astrocytic cells with gfa-i were first found in ... | 1983 | 6408144 |
| [necrotic processes in the gill tissue of carp caused by changes in the aquatic environment]. | histological findings show that various pathogenic processes are involved in the cases of clinically detected branchionecrosis. the changes in gills occurring at toxic branchionecrosis are a necrobiotic process in their nature; in gill lamellae this process manifests itself as a swelling of the respiratory epithelium and as dilation of lamellar sinuses, and in stratified epithelium of gill arch and filaments it takes on the form of the hypertrophy and hyperplasia of epithelial cells. in severe c ... | 1983 | 6419439 |
| pharmacologic profile of a novel potent direct-acting dopamine agonist, (+)-4-propyl-9-hydroxynaphthoxazine [(+)-phno]. | the (+)-enantiomer of 1,2,3,4a,5,6-hexahydro-9-hydroxy-4-n-propyl-4h-naphth[1,2-b][ 1,4]-oxazine [(+)-phno] is demonstrated to be a potent and direct dopamine (da) agonist in several in vivo and in vitro test procedures. in vitro (+)-phno inhibited binding of [3h]apomorphine (ic50 = 23 nm) or [3h]spiperone (ic50 = 55 nm) to rat striatal membranes. because (+)-phno failed to stimulate adenylate cyclase in carp retina, it was classified as a d-2 agonist. ed50 values (shown in parentheses) derived ... | 1984 | 6433000 |
| [changes in the electrolyte composition of the blood and muscles in carp infection with ichthyophthirius multifiliis (ciliata, ophryoglenidae)]. | a study of the electrolytic contents of blood serum, erythrocytes and muscles of carp infected with ichthyophthirius multifiliis was carried out. whatever the infection intensity, a characteristic increase in the cations k+ and na+ ratio in the plasma and muscles of infected fishes takes place. the above dependence, which is expressed in the intensification of active ions transport, is associated with mobilization of organism's protective functions. | 1984 | 6441141 |
| light- and electron-microscopic demonstration of immunoreactive opsin in the pinealocytes of various vertebrates. | an antibody to opsin isolated from rod outer segments of the frog retina was applied in light- and electron-microscopic immunocytochemical studies to the pineal organ of various vertebrates (cyprinus carpio, carassius auratus, rana esculenta, emys orbicularis, pseudemys scripta elegans, lacerta agilis and viridis. gallus domesticus, columba livia, melopsittacus undulatus, serinus canaria, taeniopyga punctata, didelphis virginiana, erinaceus roumanicus, myotis myotis, rabbit, rat, cat). | 1981 | 6458362 |
| stereochemistry of atp and gtp bound to fish haemoglobins. a transferred nuclear overhauser enhancement, 31p-nuclear magnetic resonance, oxygen equilibrium and molecular modelling study. | this study was undertaken in order to test the models of atp and gtp binding to carp deoxyhaemoglobin proposed by perutz & brunori (1982) and to find out why gtp is a more potent allosteric effector than atp. we have determined the conformations of both nucleoside triphosphates by nuclear magnetic resonance studies and found them to be the same. the purines are in anti conformation about the glycosidic bond that links them to the ribose; the pentose ring is 3'-endo; the p-o5'-c5'-c4' torsion ang ... | 1984 | 6492161 |
| proton nuclear magnetic resonance investigation of the allosteric transition in ligated and unligated carp hemoglobin. evidence for structural heterogeneity in the heme pocket. | the proton nuclear magnetic resonance spectra of carp hemoglobin (hb) in the unligated deoxy and ligated met-cyano and met-azido forms have been recorded as a function of ph and upon addition of inositol hexaphosphate. all protein derivatives yield spectra that are consistent with appreciable molecular heterogeneity in the heme cavity. the pattern of heme methyl hyperfine shifts in carp met-cyano hb indicates that this heterogeneity arises from the presence of heme rotational disorder, as found ... | 1984 | 6492169 |
| a field-potential study of centripetal and centrifugal connections of the olfactory bulb in the carp, cyprinus carpio (l). | the projection areas of mitral cell axons in the lateral (lot) and medial (mot) olfactory tracts and the pathways of two groups of centrifugal fibers (with fast and slow conduction velocities) to the olfactory bulb were studied in the carp using the field potential method. mitral cell axons of the lot and those of the mot projected to the overlapping areas in the bilateral telencephalon and preoptic area (poa), the contralateral mot, and the nucleus posterior tuberis (npt) in the diencephalon. h ... | 1984 | 6498513 |
| new anomalies found in the 11q-syndrome. | the cytogenetic analysis of an infant with multiple congenital anomalies revealed a small deletion of the long arm of one no. 11 chromosome: 46xx del 11 (q23-q25). the main clinical manifestations included: trigonocephaly, flat broad nasal bridge, micrognathia, carp mouth, hypertelorism, low set ears, severe congenital heart disease, anomalies of limbs and external genitalia. in comparison to the previously reported cases of 11q-, the patient presented here had congenital anomalies not described ... | 1984 | 6499268 |
| structural and functional analysis of spontaneous anti-nitrophenyl antibodies in three cyprinid fish species: carp (cyrinus carpio), goldfish (carassius auratus) and tench (tinca tinca). | high spontaneous anti-trinitrophenyl (tnp) activities were found in three cyprinid fish species: carp (cyprinus carpio), goldfish (carassius auratus) and tench (tinca tinca). the molecules involved, isolated by affinity chromatography on dinitrophenyl-lysine sepharose (dnp-lysine-sepharose), had the main characteristics of a high molecular weight immunoglobulin (igm-like). affinity measurements were performed on natural anti-dnp/tnp antibodies isolated from nine individual tench sera, using the ... | 1984 | 6500139 |
| [polymorphism of trypanosoma gruby, 1841 found in the blood of carp (carassius auratus gibelio (bloch), perch (perca fluviatilis l.) and stone perch (acerina cernua (l.))]. | | 1984 | 6516399 |
| [acid phosphatase activity in the internal organs of carp (cyprinus carpio l.) infected with tapeworm khawia sinensis (hsü, 1935) and bothriocephalus gowkongensis (yeh, 1955)]. | | 1984 | 6516402 |
| [transport of benzo(a)pyrene into animal organs following the feeding of activated sludge from swine manure]. | in feeding experiments with pigs and fish (carp and trout) the test animals received quotas of activated sludge of 7.5 and 15% dm (pigs) and 20% (carp) in their mixed feed. in an experiment with trout in an aquarium the quota of activated sludge was increased from 20% to 50%. the benzo(a)pyrene content was ascertained in selected organs of the pigs (kidney, liver, bacon, muscles) and the edible parts of the fish. as a result of this experiment one can say that the feeding of activated sludge fro ... | 1984 | 6517694 |
| magnetic susceptibility of oxy- and carbonmonoxyhemoglobins. | the room temperature magnetic susceptibilities of human and carp oxy- and carbonmonoxyhemoglobin solutions were measured in a 30-gauss (1 g = 10(-4) t) field with a superconducting magnetometer. to within experimental uncertainty, the susceptibility was the same for both the oxy and carbonmonoxy forms, and salt concentration did not effect it. a variety of sample preparations were used; the iron chemical state was verified by mössbauer spectroscopy. a value of -0.580 +/- 0.010 x 10(-6) centimete ... | 1984 | 6591198 |
| [distal 14q trisomy]. | a 22-month-old boy was found to be trisomic for distal 14q due to malsegregation of a t(10;14)(q26.3;q32.1)pat. this observation and seven others reported in the literature permit the delineation of the corresponding clinical syndrome, which includes the following : intra-uterine and postnatal growth retardation; craniofacial dysmorphism : a large facies, chubby cheeks, facial asymmetry, hypertelorism, a broad nose, a short prominent philtrum, a carp-shaped mouth, micrognathia, low-set ears with ... | 1983 | 6606379 |
| [cross-reactivity of 3m kcl-extracts of fetal tissue from various species in the macrophage electrophoresis mobility test. ii. tissue extracts from lower vertebrates in various phases of development]. | cellular sensitization was detected by the mem-test using 3m kcl-extracts of fetuses from man and mouse and of different metamorphic stages of the water frog (tadpoles) and the carp (yolk sack-bearing stage). the sensitization demonstrated was independent on the type of tumor tested. the occurrence of active components in the extracts was correlated with certain stages of individual development of water frog and carp. the evidence of these components in species of several classes of vertebrates ... | 1983 | 6606408 |
| evolution of low molecular weight immunoglobulins--iv. igy-like immunoglobulins of birds, reptiles and amphibians, precursors of mammalian iga. | radioimmunochemical studies on the comparison of the immunological cross-reactivity between the 7-s igs of birds, reptiles and amphibians (igy-like igs) and the iga of mammals (man and pig) using 125i-chicken igy and anti-chicken igy(fc) or anti-chicken igy(h) antibodies from rabbits and carp for the detection led to the conclusion that there are close antigenic relationships between them. therefore, the igy-like igs seem to be the precursors for the iga class of mammals. from that, we give a ph ... | 1984 | 6611490 |
| [peculiarities of the amino acid composition, spatial organization and interaction with dna of histones h1 from calf thymus and carp spermatozoa]. | the amino acid composition of the h1-like histone isolated from carp spermatozoa (h1carp) is characterized by a high content of lysine (34.6%) and a low content of glycine (4.5%) as compared to that of its calf counterpart (h1calf). the lys/arg ratio is 21.6, which is much higher than that for the h1-like histones from other species spermatozoa (cf. echinodermata). it was shown that the fluorescence anisotropy and excitation spectra of histones h1carp and h1calf change synchronically. at the sam ... | 1983 | 6615921 |
| lateral spread of light-induced response at the cell body and axon terminal levels of external horizontal cells in the carp retina. | one type of light-induced response (photopic l-type s-potential)recorded from isolated carp (cyprinus carpio) retinas was identified by its spectral response and later confirmed by morphological localization of the recorded sites ionophoretically marked by a fluorescent dye, lucifer yellow. such l-type s-potentials could be recorded from the soma and from the axon terminal of external horizontal cells. the spatial property of the s-potentials from the soma was compared with that recorded from th ... | 1983 | 6632374 |
| ligand binding to heme proteins. an evaluation of distal effects. | the o2, co, and alkyl isocyanide-binding properties of a variety of vertebrate and invertebrate heme proteins have been compared in detail to those of protoheme mono-3-(1-imidazoyl)-propylamide monomethyl ester in aqueous suspensions of soap micelles. the proteins examined include: cytochrome p-450cam from pseudomonas putida, beef heart cytochrome c oxidase, yeast cytochrome c peroxidase, alpha and beta subunits of human hemoglobin, sheep hemoglobin, carp hemoglobin, sperm whale myoglobin, horse ... | 1983 | 6643477 |
| evidence for the homology of hexose 6-phosphate dehydrogenase and glucose 6-phosphate dehydrogenase: comparison of the amino acid compositions. | amino acid compositions of hexose 6-phosphate dehydrogenase and glucose 6-phosphate dehydrogenase from crucian carp and rat, and that of sea-urchin glucose 6-phosphate dehydrogenase were compared in order to ascertain if they are homologous enzymes. the results demonstrated that the overall amino acid compositions of the two enzymes remarkably resemble each other, and that the composition divergence (d = 0.038) of the crucian carp enzymes is in the range of those for homologous proteins so far r ... | 1983 | 6661901 |
| scanning electron microscopic study of the intestinal mucosa of an indian freshwater adult major carp, labeo rohita (hamilton). | fine anatomical structures of the different regions (viz., anterior, middle and posterior) of the intestinal mucosa in adult labeo rohita (hamilton) have been studied by using scanning electron microscope (sem). surface mucosal modifications of the anterior and the middle of the intestine not only increase the luminal surface by 2-4 times but serve also the storage function of the ingested food due to the absence of stomach in the fish studied. fine anatomical structures of the columnar epitheli ... | 1983 | 6670351 |
| preliminary study on the effect of temperature on the transport of thyroxine (t4) into the body tissues and sub-cellular fractions of liver and muscle of carp, cyprinus carpio. | in order to determine if the inability of thyroxine to induce cellular effects at low temperature is mediated through a temperature-sensitive system for the translocation of t4 into the nucleus, the effect of temperature on the uptake of t4 by body tissues and sub-cellular fractions of carp liver and muscle was studied in vivo. a single injection of 125i-t4 (1 micro c/10 g body weight) was given intraperitoneally to juvenile carp maintained at 15 and 25 c. uptake from the peritoneal cavity was r ... | 1983 | 6670568 |
| [establishment of a fish-virus susceptible cell strain from the pituitary gland of carp (cyprinus carpio l.)]. | a new continuous cell strain (capi) was established from normal pituitary tissue of carp, cyprinus carpio l. cells of a fractionated dispersion (0.25% trypsin-pbs, 10 degrees c) of pituitaries formed monolayer (fibroblast- and epithelial-like-cells) at 25 degrees c using eagle's minimal essential medium (mem) supplemented with 10% fetal calf serum and non essential amino acids. the continuous cell strain, designated capi, was obtained by an enzymatical selection of epitheloid cells from the mono ... | 1983 | 6675352 |
| aporphines. 48. enantioselectivity of (r)-(-)- and (s)-(+)-n-n-propylnorapomorphine on dopamine receptors. | the enantiomers [(s)-(+) and (r)-(-)] of n-n-propylnorapomorphine (npa) were synthesized. (r)-npa was obtained by the acid-catalyzed rearrangement of n-n-propylnormorphine. (r)-npa also was converted to (rs)-n-n-propylnorapomorphine dimethyl ether by dehydrogenation of the 10,11-o,o'-dimethyl ether of (r)-npa with 10% palladium on carbon in acetonitrile, followed by reduction with sodium cyanoborohydride under acidic conditions. alternatively (rs)-npa 10,11-o,o'-dimethyl ether was obtained via t ... | 1983 | 6682147 |
| detection of calcium binding proteins by 45ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. | a new simple method of detecting calcium binding proteins in a protein mixture is described. a sample which might include calcium binding proteins was subjected to sds-polyacrylamide gel electrophoresis and then electrophoretically transferred to a nitrocellulose membrane. the membrane was then incubated with 45ca to detect calcium binding proteins as radioactive bands by autoradiography. purified troponin-c, calmodulin, myosin dtnb light chain, and parvalbumin were clearly identified by this me ... | 1984 | 6715311 |
| regulatory effect of dopamine on spatial properties of horizontal cells in carp retina. | three types of light-induced response (l-, rg-, and yrb -type s-potentials) recorded from isolated retinas of the carp (cyprinus carpio) were identified by their spectral response and later by morphological localization of the recording sites marked with an intracellular lucifer yellow (ly). horizontal cells in a given layer, generating one of the above response types, are electrically coupled via gap junctions, so that the injected ly normally diffused to several neighboring cells. the spatial ... | 1984 | 6726332 |
| collagen hydrolysis by a new streptomyces species. | a soil streptomycete hydrolysed collagen extracted from bovine achilles tendon, calf skin, carp swim bladder and rat tail tendon. enzyme activity was highest with rat tail tendon collagen as substrate. the taxonomy of the streptomycete was studied according to internationally approved methods. comparative studies revealed that it resembled streptomyces humidus , s. rochei and s. distatochromogenes in some respects, but differed in many cultural and physiological characteristics. based on these d ... | 1984 | 6727696 |
| oxygen equilibrium studies on carp-human hybrid hemoglobins. | hybrid hemoglobins have been made in which one chain is derived from human hemoglobin and the other from carp hemoglobin. both hybrid hemoglobins show low cooperativity in oxygen binding. hybrid i (alpha carp: beta human) has a very small bohr effect, whereas hybrid ii (alpha human: beta carp) has a bohr effect nearly as large as that for human hemoglobin. both hemoglobins have p50's more closely resembling carp hemoglobin than human hemoglobin in the region of ph 7, and for both hybrids, as for ... | 1984 | 6733079 |
| ligand binding kinetic studies on the hybrid hemoglobin alpha(carp):beta(human): a hemoglobin with a restricted allosteric range. | the preparation of native alpha- and beta-chains of carp hemoglobin and the preparation of the hybrid hemoglobin alpha(carp):beta(human), hybrid i, are described. co association and dissociation kinetics were determined for the hybrid hemoglobin alpha(carp):beta(human) as a function of fractional saturation by stopped-flow and by flash and laser photolysis. oxygen dissociation from the liganded distribution of conformers and overall oxygen association were studied by laser photolysis. an alloste ... | 1984 | 6733080 |
| ligand binding kinetic studies on the hybrid hemoglobin alpha (human):beta (carp): a hemoglobin with mixed conformations and sequential conformational changes. | oxygen and co ligand binding kinetics have been studied for the hybrid hemoglobin (hb) alpha (human):beta (carp), hybrid ii. valency and half-saturated hybrids were used to aid in the assignment of the conformations of both chains. in hybrid ii, an intermediate s state occurs, in which one chain has r- and the other t-state properties. in hbco at ph 6 (plus 1 mm inositol hexaphosphate), the human alpha-chain is r state and the carp beta-chain is t state. we have no evidence at this ph that the c ... | 1984 | 6733081 |
| primary structures of multiple forms of urotensin ii in the urophysis of the carp, cyprinus carpio. | multiple forms of urotensin ii (uii), one of the hormonal peptides of the caudal neurosecretory system of fishes, were purified from the urophyses of the carp, cyprinus carpio. three distinct peaks with uii activity (classified as uii-alpha, -beta and -gamma) were separated by reverse-phase high-pressure liquid chromatography (hplc). edman degradation as well as digestion with carboxypeptidase a revealed the primary structures of these peptides as uii-alpha: gly-gly-gly-ala-asp-cys-phe-trp-lys-t ... | 1984 | 6745627 |
| connectin content and its post-mortem changes in fish muscle. | connectin was isolated from fish dorsal myofibrils by an sds-gel filtration method and estimated to account for approximately 13% of the total myofibrillar proteins. there was no significant difference in the amount of connectin among seven fish species but rabbit skeletal myofibrils contained a slightly higher content (16%) of connectin. the high molecular weight connectins from carp and rabbit both showed a doublet band, consisting of bands 1 and 2, on sds-polyacrylamide gel electrophoresis us ... | 1984 | 6746595 |
| enzymic and molecular characteristics of a new form of monoamine oxidase, distinct from form-a and form-b. | the present study was undertaken to clarify the enzymic and molecular properties of monoamine oxidase (mao) in carp brain. in particular, its sensitivities to selective mao inhibitors, kinetic properties and molecular weight were compared with those of the enzyme in carp liver. the selective and potent mao-a and mao-b inhibitors fla 788(+), fla 336(+), md 780236 and benzylcyanide caused dose-dependent inhibitions of mao activity in both carp brain and liver; the inhibition curves were all single ... | 1984 | 6748374 |
| isolation and amino acid sequence of urotensin i, a vasoactive and acth-releasing neuropeptide, from the carp (cyprinus carpio) urophysis. | urotensin i (ui), a 41-residue mammalian hypotensive and fish or mammalian corticotropin-releasing peptide, isolated from 0.1 n hci extracts of urophyses of the carp (cyprinus carpio) was purified and the amino acid sequence was determined to be: h-asn-asp-asp-pro-pro-ile-ser-ile-asp-leu-thr-phe-his-leu-leu- arg-asn-met-ile-glu-met-ala-arg-asn-glu-asn-gln-arg-glu-gln-ala-gly-leu-asn-arg-lys-tyr-leu-asp-glu-val-nh2. when the extraction procedure included heating at 100 degrees c for 15 min, ui wa ... | 1982 | 6757895 |
| studies on the enzymatic reduction of c-nitroso compounds. i. distribution of c-nitrosoreductase activity in animal tissues and partial purification of the enzyme from porcine liver. | the subcellular distribution of nadh-p-nitrosophenol (p-nsp) reductase activity at ph 6.0 in porcine liver was studied by spectrophotometric assay. about two-thirds of the activity was found in the cytosol fraction and the ph optimum of this fraction was about 5.5. the activity at ph 5.8 of cytosol fractions from various tissues of rats, quails, frogs, carp, and scallops was also studied. all these fractions showed more or less nadh-p-nsp reductase activity but the activity of nadh-aldehyde redu ... | 1980 | 6774979 |
| dna repeat lengths of erythrocyte chromatins differing in content of histones h1 and h5. | among the erythrocytes of chicken, trout, carp, and sucker, the relative proportion of the lysine-rich histone h5 varied from 20 to 0% of the total histones. following digestion of nuclear chromatin with micrococcal nuclease, each of them displayed a longer dna repeat length and greater repeat length heterogeneity than found in liver chromatin. fish erythrocytes possessed similar repeat lengths of 207-209 base pairs which was 10-12 base pairs shorter than in chicken erythrocyte chromatin and app ... | 1980 | 6777761 |
| purification and properties of glycine decarboxylase, a component of the glycine cleavage system, from rat liver mitochondria and immunochemical comparison of this enzyme from various sources. | glycine decarboxylase, tentatively called p-protein as a constituent of the glycine cleavage system, was purified to near homogeneity from rat liver mitochondria. the purified p-protein was a homodimer with a molecular weight of about 210,000, consisting of identical subunits with a molecular weight of 105,000. in the exchange reaction of the carboxyl carbon of glycine wih co2 catalyzed by the purified p-protein in the presence of h-protein, the ph optimum was 6.7, km for glycine was 6.6 mm, and ... | 1980 | 6778858 |
| detection of a monoclonal human myeloma protein of igm and igg class by carp anti-idiotypic sera. | in the patient st. with a morbus waldenström macroglobulinemia a double paraproteinemia could be detected. besides the igm myeloma protein an igg myeloma protein was identified during the clinical course. a strong cross reactivity between the igm and the igg myeloma proteins was shown using anti-idiotypic antisera. this is an indirect indication for a common precursor cell clone of the igm- and igg-myeloma protein producing cells. the anti-idiotypic antisera were made in carp. the high specifici ... | 1981 | 6798794 |
| synthesis of diene prostaglandins in freshwater fish. | biosynthesis of pgd2, pge2 and pgf2 alpha in four species of freshwater fish, tilapia mossambica, cyprinus carpio, heteropneustes fossilis and clarius batrachus was studied. both arachidonic acid and pgh2 were used as substrates. when pgh2 replaced arachidonic acid in the enzymic reaction, there was a 3- to 4-fold increase in pge2 synthesis, but no such increase in the synthesis of pgf2 alpha and pgd2 was observed. | 1982 | 6817004 |
| presence of antigen sensitized leukocytes in carp (cyprinus carpio l) following bath immunization against flexibacter columnaris. | bath immunization of carp (cyprinus carpio l) resulted in protection of fish at natural challenge. stimulation of leukocytes derived from thymus, spleen, anterior kidney and mid-kidney of fish immunized with flexibacter columnaris bacterin revealed the presence of antigen sensitized cells in all lymphoid tissues except the anterior kidney. after 28 days a response was obtained in thymus and spleen leukocyte cultures. | 1982 | 6817508 |
| a new type of mitochondrial monoamine oxidase distinct from type-a and type-b. | the characteristics of mitochondrial monoamine oxidase (mao) in carp liver were studied with mao inhibitors and substrates. this enzyme was thermolabile, but was stabilized in the presence of bovine serum albumin. with clorgyline and deprenyl, single-sigmoidal curves for inhibition of the activity towards tyramine or 5-hydroxytryptamine were obtained; the sensitivities to the two inhibitors were identical. the activity towards beta-phenylethylamine was not completely inhibited by clorgyline or d ... | 1983 | 6823208 |
| primary structure of hemoglobin from trout (salmo irideus) amino acid sequence of the beta chain of trout hb i. | the amino acid sequence of the beta chain of trout hb i is presented; it adds to the previously reported sequence of the alpha chain (bossa et al. (1978) biochim. biophys. acta 536, 298-305), thus completing the primary structure of the hemoglobin component of trout's blood devoid of heterotropic phenomena. comparison of beta chain from trout hb i with the corresponding sequences from human and carp shows differences of 46.6% and 34.7%, respectively; the sequence (almost completed) of the beta c ... | 1983 | 6824687 |
| vertebrate skin type i collagen: comparison of bony fishes with lamprey and calf. | 1. characterization of type i collagen alpha chains, alpha 1 and alpha 2, in the skin tissues of carp and common mackerel revealed a marked interspecies difference in cnbr-peptide maps of the alpha 2 chains, suggesting the hypervariability during evolution of the alpha 2 chains relative to the alpha 1 chains. 2. when compared with calf type i collagen, lower vertebrate type i collagens derived from these bony fishes as well as from lamprey were found to exhibit a higher degree of structural simi ... | 1983 | 6839718 |
| magnetic and spectral properties of carp carbonmonoxyhemoglobin. competitive effects of chloride ions and inositol hexakisphosphate. | we have extended our studies on the magnetic properties of carp carbonmonoxyhemoglobin and the dependence of these properties upon solution variables. using an improved version of the superconducting magnetometer, we have found that the magnetic susceptibility of carp carbonmonoxyhemoglobin is sensitive to both inositol hexakisphosphate and chloride ion. the dependence upon chloride ion concentration is complex. at relatively low concentrations this anion reverses the effect of inositol hexakisp ... | 1983 | 6852007 |
| quaternary structure and spin equilibria in ferric hemoglobins. a room temperature study. | the effective magnetic moments for a number of human and carp methemoglobin derivatives were determined in solution at room temperature. the data permit us to confirm the dependence of the spin-state equilibrium of azide methemoglobin on the quarternary state of the hemoglobin and to demonstrate a similar dependence for both human and carp aquomethemoglobin. in addition, the ph dependence of the effective magnetic moment and the soret spectrum of carp azidemethemoglobin are compared. | 1983 | 6852055 |
| ionic mechanisms underlying the responses of off-center bipolar cells in the carp retina. i. studies on responses evoked by light. | off-center bipolar cells show hyperpolarizing responses to spot illumination in the receptive field center and depolarization responses to an annulus in the surround. to understand the ionic mechanisms underlying these responses, we examined the current-voltage relationship of these bipolar cells, input resistance changes during their light-evoked responses, and the reversal potentials of these responses. off-center bipolar cells generally showed inward rectification when they were hyperpolarize ... | 1983 | 6854267 |
| ionic mechanisms underlying the responses of off-center bipolar cells in the carp retina. ii. studies on responses evoked by transretinal current stimulation. | transretinal current pulses flowing from the receptor side to the vitreous side of the retina cause transient release of transmitter from the photoreceptor terminals, and in off-center bipolar cells they evoke transient depolarizations with a brief (less than 1 ms) synaptic delay. since it is known that the presence of na+ in the external medium is not essential for this type of transmitter release, we used this procedure to examine the role of [na+]o in the generation of light-evoked responses ... | 1983 | 6854268 |
| [studies on metabolism of mercury compounds in mouse, rat and carp--with special reference to biological half lives and distribution of mercury compounds in whole body and in individual organs]. | | 1983 | 6865087 |
| [effect of calcium binding on the quenching time of self-fluorescence of ca-binding proteins]. | lifetimes of phenylalanine, tyrosine and tryptophan self-fluorescence of three ca2+-binding proteins (parvalbumins pi 4.47 and 3.95 and bovine alpha-lactalbumin) in the ca2+-saturated state and without ca2+ were measured on a device functioning in a channel of synchrotron radiation of the lebedev physical institute electron accelerator c-60 with a single photon counting system. the decay curve of phenylalanine fluorescence of ca2+-saturated parvalbumin pi 4.47 is two-exponential, which results f ... | 1983 | 6871261 |
| evolution of low molecular weight immunoglobulins--iii. the immunoglobulin of chicken bile--not an iga. | studies on comparison of antigenic relationships between the immunoglobulin of chicken bile--the so-called chicken iga--and human, porcine, as well as murine iga were performed by a series of double-antibody and solid-phase ria systems using h chain-specific anti-chicken biliary ig sera and alpha chain-specific anti-human iga sera produced in rabbits and in carp. it was not possible to demonstrate a significant immunological cross-reactivity of the ig of chicken bile to the ig's of iga class. be ... | 1983 | 6877244 |
| [morphohistochemical studies of the carp intestines in bothriocephaliasis]. | morphohistochemical study of host-parasite relationships after the infection of carp with bothriocephalus opsariichthydis was conducted. it has been found out that the whole parasite's strobile comes into close contact with tissues of the mucous membrane of the carp's gut. the destruction of the gut's wall results from its digestion or lysis that is confirmed by high activity of acid phosphatase. together with epithelium of the mucous membrane of the gut exodermis of the helminth takes an active ... | 1983 | 6877860 |
| double color-opponent receptive fields of carp bipolar cells. | bipolar cells with double color-opponent receptive fields were found in the carp retina. they were classified into two types depending on their response polarity. in the receptive field center, type i cells showed hyperpolarization to a long wavelength spot (r-) and depolarization to a short wavelength spot (g+), while in the surround they showed r+, g- responses. type ii cells showed responses of opposite polarities to those of type i cells, namely r + g- in the center, and r - g+ in the surrou ... | 1983 | 6880036 |
| 5-hydroxytryptamine: its facilitative action on [3h]dopamine release from the retina. | 5-hydroxytryptamine (5-ht) at 5 x 10(-4) m caused a two-fold increase in [3h]dopamine (da) release from retinal particulate fractions of the carp (cyprinus carpio). the 5-ht action was dose- and ca2+-dependent. the three 5-ht agonists examined (5-methoxytryptamine, 5-methoxy-n,n-dimethyltryptamine and tryptamine) were more effective than 5-ht on [3h]da release. 5,6-dihydroxytryptamine was the strongest competitor among drugs tested for [3h ]da uptake, but did not evoke any significant release of ... | 1983 | 6880041 |
| characterization of the active site, substrate specificity and kinetic properties of acetyl-coa:arylamine n-acetyltransferase from pigeon liver. | it could be demonstrated that a sulfhydryl group is involved in the catalysis of acetyl-coa:arylamine n-acetyltransferase from pigeon liver (ec 2.3.1.5). from ping-pong kinetics it was concluded that there is a covalent acetyl-enzyme intermediate. the respective intermediate could be isolated and chemically characterized as a cysteinyl thioester. electrophoretically homogeneous acetyl-coa:acylamine n-acetyltransferase from pigeon liver was able to acetylate a broad variety of aromatic and alipha ... | 1983 | 6882770 |
| bioaccumulation of 95mtc in fish and snails. | concentration factors for technetium recommended in radiological assessment models for freshwater biota are default values based on the behavior of iodine in the environment. in this study a small experimental freshwater pond was spiked with 95mtc to obtain data for calculating concentration factors for fish and snails. a model using the pond data was developed to calculate steady-state body burdens for freshwater biota. the concentration factors based on the calculated body burden for carp (cap ... | 1982 | 6896045 |
| synthesis of capped and uncapped methylated oligonucleotides by the virion transcriptase of spring viremia of carp virus, a rhabdovirus. | capped and uncapped methylated oligonucleotides, five and six nucleotides in length, were synthesized in vitro in spring viremia of carp virus transcription reaction mixtures containing atp + ctp, atp + ctp + gtp, or atp + ctp + utp, but not in any other combinations of two or three ribonucleoside triphosphates. the oligonucleotides that have been characterized are consistent with the structures: m7gpppampnpcpnpn, gpppampnpcpnpn, pppampnpcpnpn, and ppampnpcpnpn--i.e., similar to those of the ter ... | 1981 | 6946423 |
| calcium-dependent interaction of s100b, troponin c, and calmodulin with an immobilized phenothiazine. | we have purified the brain-specific protein s100b by affinity-based adsorption chromatography on phenothiazine-sepharose conjugates and studied the interaction of this and other calcium-modulated proteins with the immobilized antipsychotic drug. bovine brain calmodulin, rabbit skeletal muscle troponin c, and bovine brain s100b bind to phenothiazine-sepharose in a calcium-dependent manner. these three proteins competitively inhibit the calcium-dependent binding of 125i-labeled chicken gizzard cal ... | 1981 | 6947252 |
| responses of msh and prolactin cells to 5-hydroxytryptophan (5-htp) in amphibians and teleosts. | injection of 5-hydroxytryptophan (5-htp), a precursor of serotonin, induces dispersion of melanin in the amphibians, pleurodeles waltlii (urodela) and xenopus laevis (anura), in the goldfish, carassius auratus, and in the carp, cyprinus carpio. it is accompanied by a dispersion of erythrophore pigments. in the pituitaries of pleurodeles and goldfish, a stimulation of msh cells, characterized by a significant nuclear hypertrophy, is also observed; in carassius, msh cells may become degranulated. ... | 1980 | 6967354 |
| [development of the adrenergic innervation of the atrioventricular valves of certain vertebrates]. | adrenergic innervation of the atrioventricular valves in the carp cyprinus carpo, frog rana temporaria, tortoise emys orbicularis, hens and albino rats has been investigated by histochemical glyoxylic technique. it was shown that the adrenergic innervation of cardiac valves becomes more intense and more complex during progressive evolution of cardio-vascular activity. adrenergic nerves realize the connection between annullus fibrosus, valve, chordal tendineal and papillary muscles. chromaffin ce ... | 1980 | 6967660 |
| partial monosomy of the long arm of chromosome 11 in a severely affected child. | a white female infant affected with deletion of the terminal end of the long arm of chromosome 11 (11q-) is presented. the clinical features--including trigonocephaly, epicanthal folds, coloboma of iris, prominent occiput, dysplastic ears, carp-shaped mouth, micrognathia, pectus excavalum single umbilical artery, anorectal malformation, dysplastic fifth fingers, hypoplastic nails, simian creases, hydronephrotic multicystic kidney, neutropenia, thrombocytopenia, and congenital heart anomalies--ar ... | 1981 | 6971620 |
| the haemolytic plaque assay in carp (cyprinus carpio). | a haemolytic plaque assay for the enumeration of antibody forming cells in the carp (cyprinus carpio) is described. serum of bream (abramis brama) turned out to be a more reliable complement source than allogeneic carp serum. the addition of 3--5% bream serum to a mixture of immune lymphoid carp cells and xenogeneic erythrocytes gave optimal results. higher amounts of bream complement inhibited plaque formation. plaque formation was also suppressed when inactive or heat-inactivated bream or carp ... | 1980 | 6987315 |
| [examination of some strains of the aeromonas hydrophila-punctata-group isolated from carp by resistance tests (author's transl)]. | | 1980 | 6993174 |
| cloning of carp preproinsulin cdna in the bacterial plasmid pbr322. | the successful cloning of recombinants between cdna from fractionated poly(a)+-rna of brockmann bodies of the carp and the plasmid pbr322 in escherichia coli chi 1776 is reported. one of the recombinant clones has been identified as a preproinsulin-cdna recombinant by the hybrid-arrest translation assay. recombination was at the psti site of pbr322; reconstitution of this site was by 3'-tailing of the vector with dgn. the transformants were screened by in situ hybridization with kinase-labeled p ... | 1980 | 6993287 |
| biosynthesis of proinsulin of carp (cyprinus carpio) and characterization and cloning of mrna. | | 1980 | 6994550 |
| the immune system of cyprinid fish. kinetics and temperature dependence of antibody-producing cells in carp (cyprinus carpio). | after immunization of carp with sheep red blood cells, the spleen accounts for only 5% of the total number of plaque-forming cells (pfc). in addition, thymus, peripheral blood and heart contained low numbers of pfc (< 0.5, 1 and 0.5%, respectively). pronephros and mesonephros were the major antibody-forming organs (53 and 40% of total pfc, respectively). the temperature dependence of the antibody-forming cell response in spleen, pronephros and mesonephros as studied in animals kept at 12-24 degr ... | 1980 | 7000695 |
| carp insulin: amino acid sequence, biological activity and structural properties. | the amino acid sequence of insulin of carp (cyprinus carpio) has been determined and correlated with its biological activity in a fat-cell test and its structural properties as measured by circular dichroism and sedimentation analysis. the amino acid sequence of carp insulin displays some unusual features: the b chain is longer at the n terminus by two residues as compared with mammalian insulins and there are substitutions of the charged residues, found in most insulins at positions b21 and b22 ... | 1982 | 7037403 |
| enzymatic reduction of oxidized alpha-1-proteinase inhibitor restores biological activity. | the major serum inhibitor of proteolytic activity, alpha-1-proteinase inhibitor (alpha-1-pi), (or alpha-1-antitrypsin) can be readily inactivated by oxidation [carp, h. & janoff, a. (1978) am. rev. resp. dis. 118, 617-621]. this inactivation appears to be due to the oxidation of a critical methionine(s) in alpha-1-pi that is required for the inhibition of elastase activity. an enzyme from escherichia coli that reduces methionine sulfoxide residues in protein [brot, n., weissbach, l., werth, j. & ... | 1981 | 7038679 |
| the immune system of cyprinid fish. oxytetracycline and the regulation of humoral immunity in carp (cyprinus carpio). | the antibiotic oxytetracycline (oxytc) was administered either by mixing with food or by intraperitoneal injections. on oxytc treated animals decreased serum immunoglobulin levels were found. the primary anti-sheep red blood cells (srbc) response was measured by enumerating plaque forming cells (pfc). it was observed that the pfc response was depressed by 80-95% in oxytc treated animals. when an anti-srbc serum was injected together with srbc the immunosuppressive effect of oxytc was absent. a s ... | 1981 | 7046213 |
| optimization and kinetics of in vitro stimulation of carp (cyprinus carpio l.) leukocytes. | the optimization of a leukocyte stimulation microassay with carp (cyprinus carpio l.) leukocytes is described. leukocytes were isolated from the thymus, anterior kidney, spleen, mid-kidney and peripheral blood. leukocyte cultures were stimulated with pha-p, lps (escherichia coli 055: b5) pwm, cona and ppd from mycobacterium fortuitum. the optimum incubation temperature for leukocyte cultures differed 3.5 days for leukocyte cultures derived from lymphoid organs and 4.5 days for peripheral blood l ... | 1982 | 7051523 |
| inhibition of cysteine proteinase activity by z-phe-phe-diazomethane and of aspartic proteinase activity by pepstatin in different organs from some animals and isolated cells from rat liver. | two methods have been developed to discriminate simultaneously between the main part of cysteine proteinase activity (cathepsin l) and all aspartic proteinase activity (mainly cathepsin d) in rat organs, using z-phe-phe-chn2 which at 5 mumol/l completely inhibits cathepsin l from rat liver and, on the other hand, pepstatin which at 0.5 mumol/l completely inhibits cathepsin d. substrates are double-labeled cytosol proteins from rat liver at ph 3.0 or azocasein in 3 mol/l urea at ph 5.0. several o ... | 1982 | 7051705 |
| different modes of membrane interactions of the signal sequence of carp preproinsulin and of the insertion sequence of rabbit cytochrome b5. | the signal segment of the secretory protein carp preproinsulin is shown to be bound by a protein receptor present in the rough endoplasmic reticulum membrane. the receptor does not bind the insertion segment of the integral membrane protein cytochrome b5. on the other hand, the insertion sequence, in contrast to the signal sequence, is dissolved into the lipid bilayer of natural and artificial membranes. hydrophobicity or length per se of the two types of peptides cannot be responsible for their ... | 1982 | 7067693 |
| nmr studies on parvalbumin phylogeny and ionic interactions. | the inspection of several muscular parvalbumins from different species by two nmr methods (113cd resonance and 1h relaxation measurements) allows two classes of parvalbumins to be distinguished according to their ion-binding properties. this result is in agreement with the phylogenetic classification of parvalbumins in two series, alpha and beta, which was established on the basis of the primary structures of these proteins. all parvalbumins are characterized by the presence of two primary catio ... | 1982 | 7110122 |
| radioactive deoxyglucose uptake into forelimb muscles in task performing monkeys. | incorporation of [14c]2-deoxy-d-glucose (2-dg) into forelimb muscles of task-performing monkeys was studied. extensor muscles of used forelimbs showed a significant increase in 2-dg uptake; they were ext.dig.2nd and 3rd, ext.dig.com., ext.carp.rad. and ext.carp.uln. on the other hand, flexor muscles in the used forelimb did not show such a significant increase in 2-dg uptake. muscles of non-used limbs, flexors or extensors, did not show a significant 2-dg uptake. | 1982 | 7110627 |
| toxic effects of animal manures and sewage sludge as supplementary feeds for the common carp. cyprinus carpio. | activated sludge, chicken manure and pig manure were tested for their toxic effects as supplementary feeds for the common carp, cyprinus carpio. the acute toxicity test showed that animal manures were more toxic than activated sludge (e.g., 96-h lc50 values for pig manure and chicken manure were 0.55% and 0.29%, respectively, while no effective value was revealed for activated sludge). this may be due to the inadequate processing of the manures which resulted in the release of harmful substances ... | 1982 | 7112604 |
| dendritic tree structure and dendritic hypertrophy during growth of the crucian carp eye. | the areas of the ganglion cell dendritic trees were determined in golgi-stained, flatmounted retinas of crucian carp ranging in age from one summer to 7 years. the dendritic trees of small ganglion cells (s-gc), forming the majority of retinal ganglion cells, add new branches as the retina grows. the increase in dendritic tree area exactly compensates for the decrease in ganglion cell density during growth of the eye so that the number of dendritic trees covering a particular point remains const ... | 1982 | 7130457 |
| [familial observation of partial trisomy 6, and probable partial monosomy 18q by parental translocation]. | partial trisomy 6q (6q23 leads to qter), resulting from a non-balanced translocation 6/18, was detected in a child and his paternal uncle. features of note in the uncle included micrognathia, scoliosis and mild mental retardation. the child presented a moderate hypertelorism, a flat nose bridge, carp-shaped mouth and minimal cutaneous syndactyly of the 2nd-3rd toes. these clinical signs have also been mentioned in the few cases of partial trisomy 6q described in the literature. in addition, our ... | 1982 | 7130955 |
| studies on hemoglobin intermediates. a new method for studying the reaction of hb4(co)3 with co in human, carp, rabbit and opossum hemoglobin. | | 1982 | 7131556 |
| [sarcolemmic membrane of various species: data on its enzymatic characterization and chemical composition]. | a sarcolemmal enriched fraction, f1, was obtained from the skeletal muscle of rabbit (oryctolagus cuniculus l.), hamster (mesocricetus auratus wateth.), chicken (gallus gallus l.), frog (rana ridibunda seoane), young carp (carassius carassius l.) and the marine crab (carcinus maenas l.). in this fraction, 5'-nucleotidase and na+ + k+-atpase (as membrane markers) showed higher specific activities than in the three other fractions separated after centrifugation in a discontinuous sucrose gradient; ... | 1982 | 7146587 |
| detection and localization of alkaline and acid phosphatases in the digestive system of the adult catla catla (hamilton), an indian freshwater major carp by histochemical methods. | the localization and distribution of alkaline phosphatase (alpase) and acid phosphatase (acpase) in the various regions of the alimentary canal viz., buccopharynx, oesophagus, intestine, rectum and hepatopancreas of adult catla catla have been studied by employing various histochemical techniques. alpase activity was recorded in the various regions of the alimentary canal in the present study although its intensity of localization and distribution varies greatly. the highest alpase activity was ... | 1982 | 7152228 |
| sphaerospora renicola n.sp., a myxosporean from carp kidney, and its pathogenicity. | sphaerospora renicola n.sp. is a common parasite of carp in czechoslovakia. its life cycle involves intracellular stages in the epithelial cells of renal tubuli and trophozoite stages proliferating in the tubular lumen, transforming ultimately into pansporoblasts, each having one pansporoblast nucleus and producing two spores. the spores are almost globular with an average size of 7.3 x 7.2 microns, with polar capsules of equal size, and may have two slightly protruding tubercles on their shell ... | 1982 | 7157939 |
| biology and histopathology of thelohanellus nikolskii achmerov, 1955 (myxosporea, myxozoa), a protozoan parasite of the common carp (cyprinus carpio). | thelohanellus nikolskii, a myxosporean originating from the far east, arrived in hungary through natural waters from neighbouring countries which it had originally reached because of the introduction of the amur wild carp. t. nikolskii is a specific parasite of carp which produces large cysts on the fins of fry. the cysts usually appear on the fish at the beginning of july, and in august and september cysts containing the mature spores burst. the cysts are easily seen even by the naked eye and a ... | 1982 | 7157940 |
| [the oncomiricidium of diplozoon nipponicum goto, 1891. a new type of larval haptor for the genus diplozoon (monogenea, discocotylidae)]. | diplozoon nipponicum goto 1891, a branchial parasite of cyprinus carpio is mentioned for the first time in france and western europe. carps, along with their monogenea, were introduced from hungary. after being obtained experimentally, the oncomiracidium is thoroughly studied (excretory system, ciliated cells, chaetotaxy, haptor). a new haptor for the genus diplozoon is described: two pairs of larval lateral hooklets are present. the taxonomic and phylogenic implications of these observations ar ... | 1982 | 7168525 |
| biochemical compartmentation of fish tissues. hexokinase in the brain. | differential distribution of gross, particulate and soluble forms of hexokinase in the cerebrum, cerebellum, pituitary, hypothalamus and thalamus and medulla oblongata was studied in 9 inland teleosts of different phylogenetic age to clarify the compartmentation of glucose metabolism. enzyme activity (gross) was maximum in the cerebrum and minimum in the medulla oblongata, suggesting that glucose metabolism is more intensive in the former structure. the activity in the particulate fraction was h ... | 1982 | 7187168 |
| presence of liver-forming fraction in fish egg mrnas detected by its ability to encode albumin synthesis. | poly a attached rnas were isolated from carp ovarian and mature eggs. both were equally potent in cell-free translating wheat germ system. the products were analyzed with rabbit antiserum against carp albumin and polyacrylamide gel electrophoresis. the results revealed the presence of carp albumin, a liver specific protein. | 1980 | 7190727 |
| [chromosome 18 long-arm deletion. report of 1 case]. | authors report a ten month old girl with multiple malformations: midfacial hypoplasia, hypertelorism, carp mouth, very marked antihelix, poor muscular tone, spindle-shaped fingers with a high frequency of whirles and low levels of serum immunoglobulin a. in leukocyte culture, patient shows a deletion of the long arm of a 18 chromosome. | 1980 | 7212462 |