| [immunofluorescence with the pi-3 virus--study of various working conditions for the detection of viral infection using immunofluorescence on various types of cell cultures]. | we used the fluorescence method for the investigation of the sensitivity of several kinds of cell cultures to the infection with the parainfluenza virus 3 (pi-3). cultures from calf kidneys were the most sensitive while we did not determine any differences between primary cultures and cultures of the first and second subpassages and/or freshly cultivated or incubated cultures over seven days at a temperature of 37 degrees c. equal values of infection titres like on the cultures of calf kidneys w ... | 1978 | 208220 |
| effect of zinc and other chemical agents on foot-and-mouth-disease virus replication. | chemical agents reported to inhibit the growth of various ribonucleic acid and deoxyribonucleic acid viruses were tested against foot-and-mouth disease virus in cell culture. these included zn(2+), aurintricarboxylic acid, polyribocytidylic acid, polyriboinosinic acid, phosphonoacetic acid, and the viral contact inactivator n-methyl isatin beta-thiosemicarbazone alone and with cuso(4). the most effective agent, zn(2+), inhibited foot-and-mouth disease virus production in primary calf kidney cell ... | 1978 | 208461 |
| [in vitro activity of ornidazole against anaerobic organisms. differences seen in certain strains in relation to the partial pressure of oxygen. (author's transl)]. | | 1978 | 209402 |
| the clinical importance of anaerobic bacteria in wound infections after gastrointestinal surgery. | wound cultures from 54 patients with infections after gastrointestinal surgery were examined. cultures from wounds after surgery on the upper gastrointestinal tract grew few organisms, mainly aerobic gram-positive cocci. culture from wounds on the lower gastrointestinal tract grew strains of bacteria, aerobic and anaerobic gram-negative rods dominating. indirect immunofluorescence studies on acute and convalescent phase sera showed significant immune response against bacteroides fragilis in a ma ... | 1978 | 209532 |
| [infections by anaerobic bacteria]. | | 1977 | 211568 |
| decrease of rat liver cysteine dioxygenase (cysteine oxidase) activity mediated by glucagon. | the hepatic cysteine dioxygenase activity of rats was markedly decreased by the intraperitoneal administration of glucagon. the enzyme activity was also decreased by either dibutyryl cyclic amp or theophylline. the prior administration of actinomycin d completely blocked the glucagon-mediated decrease of enzyme activity, while administrations of this inhibitor of protein synthesis after glucagon injection did not block the decrease of enzyme activity. a single administration of actinomycin d res ... | 1978 | 212416 |
| the synthesis of complex-type oligosaccharides. i. structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus g protein. | the synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus g protein is initiated by the en bloc transfer of a high molecular weight oligosaccharide from a lipid carrier to the nascent polypeptide. following transfer the oligosaccharide is "processed" by removal of glucose and mannose residues and the sugars that constitute the outer branches of the complex-type oligosaccharide are added. the structure of the oligosaccharide moiety of the lipid-linked precursor has ... | 1978 | 212434 |
| suppression of interferon production in mouse spleen cells by cytochalasin d. | cytochalasin d is thought to impair microfilament function. the present study has investigated its effects on four different systems in which interferon is formed, namely (1) mouse fibroblasts induced with virus (2) mouse spleen cells induced with virus, or (3) with endotoxin or (4) by allogeneic stimulation. cytochalasin d did not suppress formation of interferon by fibroblasts (l cells) or spleen cells stimulated with either hvj or ndv. however it did suppress production of interferon by splee ... | 1978 | 212512 |
| incomplete anaerobiosis as a cause of metronidazole 'resistance'. | clostridium welchii, used as a control, was found to grow well in a microaerophilic jar used for campylobacters but appeared resistant to a metronidazole disc although the campylobacter was sensitive. minimum inhibitory concentrations for six strains of cl. welchii were up to 64-fold higher in these conditions than when grown anaerobically. zones of inhibition with both cl. welchii and bacteroides fragilis varied with the amount of air admitted to anaerobic jars. | 1978 | 213449 |
| studies on secretory glycoproteins in the rat exocrine pancreas. iii. intracellular transport of fucose-labeled proteins as studied by cell fractionation. | the transcellular movement of fucosylated glycoproteins has been studied in vitro using rat pancreatic lobules and cell fractionation procedures, and has been compared with the well established pathway of secretory proteins. using tritiated leucine as pulse label for the latter, their translocation from the rough endoplasmatic reticulum into the golgi complex and finally into zymogen granules could be followed. in the case of glycoproteins, 14c-fucose was incorporated mainly into the smooth micr ... | 1978 | 214234 |
| linkage of the endogenous avian leukosis virus genome of virus-producing chicken cells to inhibitory cellular dna sequences. | the endogenous avian leukosis virus genome of uninfected virus-producing(v+) chicken embryo fibroblasts appears to be inefficiently transcribed and is noninfectious in transfection assays of high molecular weight dna. we previously proposed that the endogenous virus genome was linked to a cis-acting control element which inhibited its transcription and, consequently, interfered with transfection by endogenous viral dna. we now report that dna of uninfected v+ cells is infectious at a low level w ... | 1978 | 214243 |
| a fatal enteric syndrome in mystromys albicaudatus (white tailed rat) following topical antibiotic treatment. | heavy death losses (59%) occurred in adult mystromys 3--14 days after muscle biopsies were taken from their rear legs. clinical signs included anorexia, depression, and rough hair coat. predominant necropsy findings were hrmorrhagic typhlitis and colitis. results of studies evaluating the roles of the anesthetic and the tropical antibiotic ointment suggested that the deaths were caused by ingestion of the topically applied antibiotic. | 1978 | 214636 |
| isolation and partial characterization of secreted mouse pituitary prolactin. | prolactin secreted by mouse anterior pituitaries was purified by gel filtration on sephadex g-100. electrophoretic homogeneity of the purified hormone was demonstrated in several gel systems, and electrophoresis in the presence of sodium dodecyl sulfate indicated an apparent molecular weight of 21 000 +/- 2000. mouse and ovine prolactin displayed parallel dose vs. response curves in radio-receptor binding studies, indicating that these two hormones compete for identical receptor sites on rabbit ... | 1978 | 215218 |
| [detection of tubercular infection after bcg vaccination]. | the author has followed several groups of children over a period of many years and evidenced the general decrease of the level of tuberculin reactivity. the implications are discussed, of the b.c.g. vaccination on the tuberculous allergy and the possibility to differentiate post-vaccinal allergy with the aid of skin testing, using 2 and 10 ppd units. the importance is stressed, of the vaccinal scar as an indication of a good immune reactivity, and thus of protection against contamination with vi ... | 1978 | 217060 |
| influence of early anosmia on the developing olfactory bulb. | | 1978 | 218250 |
| low trimethoprim susceptibility of anaerobic bacteria due to insensitive dihydrofolate reductases. | all the 28 bacteroides fragilis strains investigated were susceptible to sulfamethoxazole (minimal inhibitory concentration < 16 mug/ml) and resistant to trimethoprim (tmp; minimal inhibitory concentration > 4 mug/ml). synergism between sulfamethoxazole and tmp was present in all strains at a ratio of 1:1. the few clostridia investigated proved more resistant to both compounds. dihydrofolate reductases from b. fragilis, c. perfringens, and some other anaerobic species were isolated. inhibition p ... | 1979 | 218496 |
| mechanism of aminoglycoside antibiotic resistance in anaerobic bacteria: clostridium perfringens and bacteroides fragilis. | cell-free amino acid incorporation using ribosomes from strains of either clostridium perfringens or bacteroides fragilis was shown to be susceptible to inhibition by streptomycin and gentamicin. ribosomes bound dihydrostreptomycin as effectively as ribosomes from escherichia coli. no inactivation of streptomycin or gentamicin was detected by cell extracts of either anaerobic bacterial species. b. fragilis, grown without added hemin, menadione, and fumarate, and c. perfringens did not show any t ... | 1979 | 218500 |
| presence of viral particles in the salivary gland of calomys musculinus infected with junin virus by a natural route. | calomys musculinus, a wild cricetid rodent, is one of the main reservoirs of junin virus. six of these animals were infected by being placed in close contact with animals that had been experimentally infected with the virus. they were sacrificed at 10, 15 and 20 months after contact, and their salivary glands were studied by ultrastructural, immunohistochemical and virological methods. two animals developed chronic viremia and low titers of complement-fixing antibodies. these animals were the on ... | 1979 | 218895 |
| recognition of viral antigens in 6/94 virus-induced t-cell-mediated cytotoxicity. | distinct events in the virus-stimulated t-cell-mediated cytotoxicity (v-cmc) have been investigated: 1.) the induction of v-cmc is possible by immunizing mice with infectious as well as uv-inactivated virus (parainfluenza type 1 strain 6/94), or with virus-infected cells either compatible or imcompatible with the recipient. 2). recognition of viral antigens by the effector cells occurs independently of the h2 environment: fractionation of effector cells on columns loaded with virus-infected cell ... | 1979 | 219322 |
| inconsistency of collagenase activity for isolation of islet cells for transplantation. | | 1979 | 219568 |
| characterization of a murine myeloma (mopc-315) c-type virus by nucleic acid hybridization. | | 1979 | 219600 |
| intestinal strangulation in germfree and monocontaminated dogs. | earlier studies in the germfree rat demonstrated that the common intestinal bacteria vary greatly in their ability to cause death after intestinal strangulation. some of these experiments were repeated in adult, germfree and monocontaminated beagles. neither short closed-loop hemorrhagic nor long closed-loop ischemic strangulation killed the germfree dog. either procedure rapidly killed the dog with a conventional bacterial flora or a dog monocontaminated with clostridium perfringens. the dogs m ... | 1979 | 219816 |
| humoral antibodies to mouse mammary tumor virus in sera from breast cancer patients. | | 1978 | 220186 |
| ornidazole and anaerobic bacteria: in vitro sensitivity and effects on wound infections after appendectomy. | the sensitivities of 68 clinical isolates of bacteroides fragilis, 18 of clostridium perfringens, and 11 of other clostridium species were tested against ornidazole alone and in combination with ampicillin and gentamicin. a concentration of 3.1 microgram of ornidazole/ml inhibited 98% of the strains of b. fragilis, with greater sensitivity when ampicillin and gentamicin were also present. a concentration of 6.2 microgram of ornidazole/ml inhibited 16 of 18 strains of c. perfringens and all 11 st ... | 1979 | 220342 |
| metronidazole bioassay with increased sensitivity. | the bioassay of metronidazole using clostridium butyricum incorporated in agar plates detected concentrations higher than 1.0 microgram/ml. gentamicin in the sample did not affect the growth of the target organism or the inhibition by metronidazole. penicillin in the sample could be eliminated by the incorporation of penicillinase in the agar or by using as the target organism a surface inoculum of the penicillin-resistant bacteroides fragilis. increasing duration and temperature of aerobic pred ... | 1979 | 220515 |
| concomitant t- and tk-activation associated with acquired-b antigens. | three patients with acquired-b antigens are reported whose red blood cells also manifested t- and tk-activation. the results of tests using purified arachis hypogaea and bandeiraea simplicifolia (bs ii) lectins, and red blood cells either t, tk or both t and tk-activated by bacterial enzymes in vitro, suggest that the acquired-b phenomenon, t-activation and tk-activation result from the action of different bacterial enzymes. | 1979 | 222015 |
| subacute cadmium intoxication in jewelry workers: an evaluation of diagnostic procedures. | an outbreak of cadmium intoxication in a jewelry factory provided an opportunity for evaluating the usefulness of diagnostic procedures used to evaluate human cadmium toxicity. blood cadmium levels in workers exposed to cadmium were higher (.93 micrograms/100ml vs .38 micrograms/100 ml) than in unexposed workers. a dose-response relationship was noted between blood cadmium level and symptom prevalence in four symptoms (dyspnea, chest pain, dysuria, and dizziness). segmental hair analysis reveale ... | 1979 | 222221 |
| investigation of the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease. | the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease with an intestinal fistula was investigated with various serological techniques. two aerobic bacterial species, e. dispar and p. mirabilis, and four strict anaerobic bacterial species, b. fragilis ss. fragilis, f. varium and two different strains of c. perfringens, were isolated from fistula secretion of the patient. these strains were used as antigens for tube agglutination, passive hemagglutinati ... | 1979 | 223235 |
| reciprocal responses to tilt of neurones within both fore- and hindlimb regions of deiters' nucleus. | in decerebrate cats with cerebellum intact the frequency response of 102 neurons located within the lateral vestibular nucleus (lvn) to sinusoidal stimulation of vestibular receptors was analyzed. positional sensitive units, showing a reciprocal pattern of response to lateral tilting, characterized by an excitation during ipsilateral and a depression during contralateral tilt, were equally found in the rostroventral (forelimb) and dorsocaudal (hindlimb) divisions of the lvn. no unit was found to ... | 1979 | 228242 |
| activity of semisynthetic penicillins and synergism with mecillinam against bacteroides species. | the minimal inhibitory concentrations (mic) of six penicillins (ampicillin, carbenicillin, ticarcillin, piperacillin, mezlocillin, and bay k 4999) against 29 clinical isolates of bacteriodes spp. (including bacteroides fragilis, bacteroides thetaiotaomicron, and bacteroides vulgatus) were determined by an agar dilution method. bay k 4999 was most active, followed in descending order by ampicillin, piperacillin, mezlocillin, ticarcillin, and carbenicillin. mecillinam, a 6 beta-amidino-penicillani ... | 1979 | 228593 |
| interspecific heterokaryons between oocytes and blastomeres of the mouse and the bank vole. | interspecific hybrid cells were produced using sendai-virus-mediated fusion of interphase blastomeres from 2-cell embryos with ovulated oocytes of the mouse and bank vole. under the influence of the cytoplasm of oocytes (arrested in metaphase ii) premature chromosome condensation occurs in the nuclei of blastomeres, thus implying that the cytoplasmic factor which induces chromosome condensation is not species specific, at least not between these two species. | 1979 | 229193 |
| anchorage independence: analysis of factors affecting the growth and colony formation of wild-type and dl 54/59 mutant sv40-transformed lines. | | 1979 | 229626 |
| experimental animal models for anaerobic infections. | an experimental animal model that stimulates the mixed aerobic-anaerobic microflora of intraabdominal sepsis was used to study antimicrobial efficacy in vivo. treatment of infected rats with chloramphenicol resulted in only a modest reduction in the percentage of animals surviving infection with abscesses at necropsy. this unanticipated observation led to further exploration of the predominant anaerobes associated with the experimental infection. in vitro cultures of bacteroides fragilis, suscep ... | 1979 | 232936 |
| antimicrobial activity of metronidazole in anaerobic bacteria. | the antimicrobial activity of metronidazole was investigated in anaerobic bacteria by use of time-viability studies. this antimicrobial agent has a rapid onset of bactericidal activity under proper reducing conditions. the bactericidal rates were not affected by inoculum size or nutritional requirements, nor by inhibition of growth and protein synthesis by chloramphenicol. using supernatant fractions of actively growing cultures of susceptible organisms, we observed a disappearance of metronidaz ... | 1978 | 233729 |
| effects of temperature on diphosphopyridine nucleotide-linked isocitrate dehydrogenase from bovine heart. aspects of the kinetics, stability, and quarternary structure of the enzyme. | a temperature-dependent conformational change of the active dpn-linked isocitrate dehydrogenase was observed. when initial reaction kinetic data were examined between 35 and 5 degrees, the hill number (n) varied from 2 at higher to n approaching unity at lower temperatures, with an inflection point at 17 degrees. the presence of manganous isocitrate in the incubation media shifted the transition temperature for enzyme inactivation by 5,5'-dithiobis(2-nitrobenzoate) from 8-16 degrees. these tempe ... | 1975 | 234954 |
| the esterase activity of bovine carbonic anhydrase b above ph 9. reversible and cooalent inhibition by acetozolamide. | the reversible complex between the metalloenzyme bovine carbonic anhydrase b and the sulfonamide inhibitor acetazolamide can be "frozen" irreversibly by the addition of a covalent bond between the methyl group of the inhibitor and the tau-nitrogen of histidine-64. in both cases the inhibited enzyme is inactive as an esterase toward p-nitrophenyl propionate at physiological ph but retains activity controlled by an ionization in the protein exhibiting a pk-a greater than 10. similarly, both the co ... | 1975 | 235550 |
| diuretics, urate excretion and sodium reabsorption: effect of acetazolamide and urinary alkalinization. | the uricosuric properties of acetazolamide were investigated in order to elucidate the relationship between changes in proximal tubular sodium reabsorption and urate excretion in man. acetazolamide produced a modest uricosuric response which was not suppressible by pyrazinamide. alkalinization of the urine with sodium bicarbonate elicited an even smaller increment in the urate clearance. if urinary alkalinization does play a role in the uricosuric response to acetazolamide, it probably decreases ... | 1975 | 235746 |
| multiple forms of 7-alpha-hydroxysteroid dehydrogenase in selected strains of bacteroides fragilis. | multiple forms of 7-alpha-hydroxysteroid dehydrogenase were detected in six of nine strains of bacteroides fragilis. the enzymes differed with respect to pyridine nucleotide specificity, thermal stability, divalent metal cation requirement, and elution profilies from sephadex g-200 columns. the nicotinamide adenine dinucleotide phosphate (nadp)-dependent enzyme required divalent metal cations, preferentially mn-2+ (km, 57 mum), for maximum catalytic activity. the nadp-dependent enzyme was labile ... | 1975 | 236279 |
| production of cell-bound antigens by bacteroides fragilis nctc 9343 in continuous culture. | highly active antigen preparations, as measured by precipitation and inhibition of haemaglutination, were obtained from bacteroides fragilis nctc 9343 grown in continuous culture. the activity varied slightly only with the glucose concentration of the medium and the dilution rate. ph had a greater influence on antigen production. | 1975 | 236632 |
| nad- and nadp-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis. | twenty strains of bacteroides fragilis were screened for hydroxysteroid oxidoreductase activity in cell-free preparations. eighteen strains were shown to contain nad-dependent 7alpha-hydroxysteroid dehydrogenase. sixteen of the strains containing the nad-dependent enzyme also contained nadp-depedent 7alpha-hydroxysteroid dehydrogenase, but invariably in lesser amounts. a strain particulary rich in both 7alpha-hydroxysteroid dehydrogenase activities was selected for further study. measurement of ... | 1975 | 236764 |
| acid-base changes and excitation-contraction coupling in rabbit myocardium. ii. effects on resting membrane potential, action potential characteristics and propagation velocity. | the effects of changes in acid-base parameters on the resting membrane potential, action potential characteristics and propagation velocity were studied in isolated rabbit papillary muscles. lowering extracellular ph from 7.4 to 6.7 in a bathing solution buffered with 10 mm histidine did not alter the resting membrane potential or action potential characteristics but casused slight reduction in propagation velocity. a parallel increase in hco3-minus concentration (up to 47 mm)and pco2 at a const ... | 1975 | 238360 |
| susceptibility of bacteroides fragilis to spectinomycin. | | 1975 | 240793 |
| survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems. | the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ... | 1975 | 240870 |
| a ligand-induced conformational change of yeast dipeptidase at physiological ph: kinetic consequences and possible regulatory significance. | the hydrolysis of dipeptides by purified yeast dipeptidase (ec 3.4.13.?) shows marked deviations from michaelis-menten kinetics over a wide range of ph. quite anomalous kinetics is observed between ph 6 and 7, indicating a drastic change in the enzyme's properties. a reasonable explanation is provided by the assumption of a conformational transition brought about either by ph shifts or, at a constant ph, by changes in the substrate concentration. the transition, which may have a half-life on the ... | 1975 | 241695 |
| microbial kinetics of drug action against gram-positive and gram-negative organisms. ii: effect of clindamycin on staphylococcus aureus and escherichia coli. | clindamycin-affected staphylococcus aureus cultures show biphasic steady-state generation curves. an initial (phase i) generation of the clindamycin-affected staph. aureus is followed by an ultimate (phase ii) generation at the same dose level. the phase i apparent generation rate constant is greater than the phase ii apparent generation rate constant and suggests the development of resistant staph. aureus mutants to clindamycin action after a finite period of drug-bacteria contact at any subcom ... | 1975 | 241826 |
| [report of "lethal factor" synthetized by bacillus cereus (author's transl)]. | bacillus cereus growing in nutrition broth produces during logarithmic growth and in stationary phase a lethal factor extremely toxic for rodents. this toxin is only produced in the presence of oxygen and depends on bacterial replication. the highest titers of toxin are obtained using bacteria in a concentration of 10(7) to 10(9) per ml of nutrition broth. during regression phase of bacillus cereus the titer of toxin declines to zero. toxicity of the lethal factor becomes not altered in an alcal ... | 1975 | 242167 |
| [the combination effect of ampicillin and dicloxacillin on the strains of bacteriodes fragilis isolated from clinical specimens (author's transl)]. | the antimicrobial activities of ampicillin (abpc) and dicloxacillin (mdipc) alone were tested in 100 strains of b. fragilis isolated from clinical specimens, and combined activity of abpc and mdipc was investigated employing a chequer board titration method (box method) with agar plates and liquid media. the following results were obtained. 1. of the 100 strains tested, approximately 33% were shown to have mics of abpc greater than 100 mcg/ml, whereas 92% of strains were greater than 100 mcg/ml ... | 1977 | 242607 |
| susceptibility to beta-lactam antibiotics and production of beta-lactamase in bacteroides fragilis. | using the agar dilution technique, 231 strains of bacteroides fragilis, isolated during a 2-year period from human infections, were identified at subspecies level and were tested for susceptibility to 13 beta-lactam antibiotics. the penicillins were benzylpenicillin, ampicillin, carbenicillin, cloxacillin, and the recently described penicillin derivatives cyclacillin, ticarcillin, and pc-904. the following cephalosporin derivatives were tested: cephaloridine, cephalothin, cephalexin, cefamandole ... | 1977 | 243112 |
| [synergistic effect of ampicillin and dicloxacillin against bacteroides fragilis (author's transl)]. | | 1978 | 246041 |
| cefuroxime, a beta-lactamase-resistant cephalosporin with a broad spectrum of gram-positive and -negative activity. | the in vitro activity of cefuroxime, a cephalosporin antibiotic, was investigated against 604 isolates and compared with the activity of other beta-lactam compounds. cefuroxime had activity comparable to that of other cephalosporins, including cefamandole and cefoxitin, against streptococcal and staphylococcal species; most streptococci were inhibited by 0.1 mug or less per ml, and staphylococci were inhibited by 1.6 mug or less per ml. enterococci were relatively resistant. cefuroxime inhibited ... | 1978 | 248268 |
| infective endocarditis: part ii. current therapy. | | 1978 | 249230 |
| carbenicillin for treatment of bacteroides fragilis infections: why not penicillin g? | carbenicillin has been advocated for treatment of infections caused by bacteroides fragilis and other anaerobic bacteria. wide-scale use of the drug in this setting could result in a substantial increase in carbenicillin-resistant pseudomonas aeruginosa, an effect that would have serious implications. thirty-four strains of b. fragilis, one-half from bacteremic infections, were tested in vitro, and penicillin g was found to be twice as active as carbenicillin on an equal weight basis; 94% of the ... | 1978 | 253006 |
| [comparison of the antibacterial activity of ticarcillin with other antibacterial agents (author's transl)]. | antibacterial activity of ticarcillin was determined in comparison with that of sulbenicillin, amoxicillin, cefuroxime, clindamycin and metronidazole against anaerobic bacteria which have been isolated from various clinical materials in this hospital. growth of more than 90% of gram-negative anaerobic rod bacteria was inhibited by ticarcillin at its concentration of 100 micrograms/ml. strains resistant to ticarcillin showed cross resistance against both sulbenicillin and amoxicillin. antibacteri ... | 1979 | 258675 |
| the effect of indomethacin on cardiovascular and lysosomal mechanisms during experimental myocardial ischemia. | the present study evaluated the effects of indomethacin (indo) on cardiovascular and lysosomal mechanisms during experimental myocardial ischemia (mi). indo (5 mg/kg) was infused iv prior to mi, and the results were compared to vehicle-treated and sham-operated dogs. indo induced a slight hypertension after mi. additionally, indo attenuated the decline in coronary blood flow in the non-ischemic myocardium after mi. myocardial lysosomes (biopsied three hours post-mi) were more labile in ischemic ... | 1978 | 262078 |
| the effect of arachidonic acid on the response of the isolated liver to hypoxia. | arachidonic acid, the precursor molecule of the bisenoic series of prostaglandins, was investigated for its ability to modify the response of isolated perfused livers to hypoxia. rabbit livers were perfused at a constant rate of flow with krebs-henseleit bicarbonate buffer for two hours following the initiation of severely hypoxic conditions. this procedure resulted in significant increases in the wet weight of the liver, and also resulted in the release of intracellular enzymes into the perfusi ... | 1978 | 262082 |
| effects of herbs and drugs during pregnancy and lactation. | prior to considering the effects of herbs and drugs during pregnancy and lactation, attention is directed to the relevance of herbal medicines to human patients. when a drug is taken orally, its absorption, biotransformation, and excretion will affect its blood level. it then makes its way to the capillaries surrounding the alveoli of the breast, and the drug must cross the endothelium of the capillaries into the alveolar cells of the breast and then be excreted into the lumen with the milk. ... | 1979 | 262485 |
| experimental studies on the biological properties of bordetella pertussis. | | 1979 | 262507 |
| decline of maternal antibodies to plague in norway rats. | the decline of maternal antibodies to the fraciton i antigen of yersinia pestis was investigated in newly weaned rattus norvegicus obtained from dams vaccinated with strain ev76(51f) of y. pestis. iha titre decreased by 50% each 7-3 days and cf titre declined 50% each 10-0 days in young rats. an analysis of available data indicated that maternal iha and cf antibodies could persist to 3 months of age. therefore, positive serologic reactions in young r. norvegicus, detected in the course of serolo ... | 1977 | 264498 |
| [use of the tray system in dental practice]. | the present account of experience presents the commercial trya assemblage and illustrates some important possiblities of using it. it is emphasized that trays should be introduced only after individual work analysies. in this connexion, it is concluded that any tray assemblage must be in individual one. | 1977 | 265644 |
| [actinomyces in dental plaque]. | | 1977 | 266558 |
| [submicroscopic studies of experimental pulp mineralizations]. | | 1977 | 268278 |
| serological studies of bacteroides fragilis. | using direct agglutination methods, a simple serological scheme for the classification of bacteroides fragilis is described. twenty strains of b. fragilis were selected by a process of sucessive screening from 151 strains obtained from various sources. o-antigens were prepared from the 20 strains, and used to raise antisera in rabbits. each of the 20 antisera reacted with its homologous antigen and eight antisera cross-reacted with other subspecies. these cross-reactions were successfully remove ... | 1977 | 269199 |
| [physiological significance of human apolipoproteins]. | | 1977 | 269614 |
| pyrolysis gas chromatography of microorganisms. influence of various pyrolysis parameters on the yield of volatile organic products. | bacteroides fragilis was analysed by pyrolysis gas chromatography (pgc). the influence of temperature rise time, pyrolysis temperature and pyrolysis time on the yield of volatile organic pyrolysis products was studied. the use of short pyrolysis time (8 msec) and a high pyrolysis end temperature (1300 degrees c) was found to provide a high yield. in consecutive analyses of the test organism, under the pyrolysis conditions indicated, the standard deviation of the total yield of volatile organic p ... | 1977 | 269647 |
| nonrandom chromosome rearrangements in 27 cases of human myeloid leukemia. | the r-banding pattern of the chromosomes of 31 patients hospitalized in the hematologic clinic for myeloid leukemia were studied before chemotherapy. this analysis permitted identification of one unusual 3-chromosome rearrangement t(3;9;22) in addition to 25 classic forms of (22q-;9q+) translocation accompanied by the specific ph' chromosome in chronic granulocytic leukemia patients, independent of the blastic course of the disease. during blastic crisis observed in 6 patients, extra 8 and 10 ch ... | 1977 | 271632 |
| liposome--lymphocyte interaction: saturable sites for transfer and intracellular release of liposome contents. | the water-soluble dye 6-carboxyfluorescein was trapped in the internal aqueous compartments of small sonicated dioleoyl lecithin vesicles and used to assess the kinetics of transfer of vesicle contents to human lymphocytes. by using flow microfluorometry, the initial rate of dye transfer to the cells was measured as a function of the concentration of vesicles in the external medium. the rate of transfer consists of at least two components, one of which saturates at high vesicle concentration and ... | 1977 | 271988 |
| pharmacological studies on an iron-poly-(sorbitol-gluconic acid) complex for parenteral treatment of iron deficiency anaemia. | intravenous injection of the iron-poly(sorbitol-gluconic acid) complex (ipsg) to cats anaesthetized with sodium pentobarbitone caused a transient decrease in mean arterial blood pressure and a temporary increase in central venous pressure, heart rate and femoral blood flow at large doses (cumulative doses up to 744 mg/kg). tachyphylaxis developed upon repeated administration. a temporary reduction in the magnitude of the blood pressure responses to noradrenaline and isoprenaline was obtained aft ... | 1977 | 272036 |
| tetracyclines in gynecologic infections. | the use of tetracyclines in salpingitis and in postsurgical gynecological infections is outlined. unless the patient is allergic, the drug of choice in gonococcal salpingitis is penicillin or a derivative. however, a study of outpatients with gonococcal salpingitis showed that a majority of 197 women studied became asymptomatic 5 days after starting tetracycline treatment. in nongonococcal acute salpingitis, evidence is good that both aerobic and anaerobic organisms are usually involved, espe ... | 1978 | 272213 |
| effect of hypophysectomy on dentin production in maxillary incisors in the rat. | | 1978 | 274803 |
| [report on the capsule dosage and mixing system. 3. suitability testing from a technological and clinical point of view]. | the results from experiments with composite filling materials, silicate cements, stone cements, polyacrylate cements, eba cements and amalgam testify to the suitability of a mixing apparatus already presented (cf. par ii). apart from industrial predosed products, well known multicomponent materials prepared until now manually, among which evicrol, were mixed mechanically. | 1978 | 274850 |
| the ultrastructure of human osteosarcoma: a study of nine cases. | electron microscopic study of 9 osteosarcomas corroborates the light microscopic observation that cells with osteoblastic, chondroblastic and fibroblastic features occur in osteosarcomas. two types of multinucleated giant cells were observed, one was osteoclast-like. the other had anaplastic and degenerative changes. the intercellular matrix is composed of collagen fibers, amorphous ground substance with osteoid and tumor bone. contrary to many previous electron microscopic reports, osteosarcoma ... | 1978 | 275014 |
| recent trends in salmonella and shigella in new york city and at kings county hospital. | | 1979 | 283866 |
| fractionation of glucans synthesized by streptococcus mutans in vitro. | | 1979 | 283884 |
| collagen reduces glycosaminoglycan degradation by cultured mammary epithelial cells: possible mechanism for basal lamina formation. | collagenous substrates are reported to promote the accumulation of extracellular matrix materials by epithelia in culture. glycosaminoglycan (gag) metabolism is compared in secondary cultures of mouse mammary epithelial cells maintained on plastic or type i collagen gel substrates. the incorporation of 35so42- into gag during brief labeling indicates no difference between substrates in the rate of gag synthesis. during prolonged labeling, however, accumulation of [35s]gag in cultures on colllage ... | 1979 | 284399 |
| effects of isolation in male and female rats: shock-elicited behaviour, pellets, water and saline intake, plasma and brain biochemistry and organ weights. | | 1978 | 284564 |
| [acute dental care in stockholm municipality]. | | 1978 | 284615 |
| bilateral reticular formation lesions causing coma: their effects on regional cerebral blood flow, glucose utilization and oxidative metabolism. | | 1978 | 284730 |
| experimental bacteroides fragilis abscess in the rat liver. | liver abscess was induced in rats by injecting a culture of b. fragilis ss. fragilis (approx. 5x10(6) cells) into devascularized liver tissue. mixed infection with b. fragilis and aerobes occurred in all animals subjected to repeated laparotomies for bacteriological sampling (4, 7, 14 and 28 days after inoculation). in animals sampled only at 8 or at 42 days after injection, b. fragilis was isolated in pure culture from the majority of animals. the size of abscesses containing b. fragilis in pur ... | 1979 | 288164 |
| the humoral antibody response to bacteroides fragilis infections in humans. | the humoral antibody response to bacteroides fragilis infections in humans, with particular reference to ss. fragilis, was studied using an enzyme immunosorbent assay (eia). phenol-water extracted polysaccharide fractions (ps) from b. fragilis ss. fragilis, ss. ovatus, ss. distasonis and ss. vulgatus were used as antigens. antibody titer determinations were done on sera collected from 57 patients where b. fragilis had been cultured and from 50 controls. in patients with septicemia caused by b. f ... | 1979 | 288165 |
| anaerobic bacteria in maxillary sinusitis. | the anaerobic bacterial flora of purulent maxillary sinusitis was investigated in 30 patients and the different anaerobic species identified and tested with respect to antibiotic susceptibility. most strains were identified as anaerobic cocci. non-sporeforming gram-positive rods and gram-negative rods other than bacteroides fragilis were also isolated. the majority of the strains were susceptible to benzylpenicillin, cefoxitin, clindamycin, doxycycline, metronidazole and chloramphenicol. | 1979 | 288166 |
| alterations of host defenses paralleling cholesterol-induced atherogenesis. i. interactions of prolonged experimental hypercholesterolemia and infections. | cholesterol may interfere with normal repair processes in the arterial wall. it is also possible that this same action occurs during inflammation and repair throughout the body. this hypothesis was tested by giving intravenous injections of bacteroides fragilis, a bacterium that is innocuous for normal rabbits even when multiple large doses are given. almost all cholesterol-fed rabbits developed significant pleuropulmonary infections with complications that included vasculitis, thrombosis, extra ... | 1979 | 290725 |
| effect of intestinal bacteria on incidence of liver tumors in gnotobiotic c3h/he male mice. | the effect of intestinal microflora on liver tumorigenesis was studied in gnotobiotic c3h/he male mice monoassociated, diassociated, or polyassociated with the following strains of intestinal bacteria: escherichia coli, streptococcus faecalis, bifidobacterium adolescentis, bifidobacterium infantis, clostridium indolis, c. paraputrificum, c. perfringens, c. innocuum, c. nexile, c. ramosum, c. clostridiiforme, bacteroides multiacidus, bacteroides fragilis, veillonella alcalescens, v. parvula, and ... | 1979 | 292809 |
| comparison of in vitro activity of cephalexin, cephradine, and cefaclor. | inhibitory activity of cephalexin, cephradine, and cefaclor was compared by the who-ics agar dilution technique. cefaclor was substantially more active against staphylococci, streptococci, gonococci, meningococci, haemophilus, escherichia coli, klebsiella pneumoniae, citrobacter diversus, proteus mirabilis, salmonellae, and shigellae than was cephalexin, which in turn was more active than cephradine. cefaclor appeared to be less resistant to staphylococcal penicillinase than did the other two ag ... | 1977 | 301005 |
| studies with cefuroxime and cefoxitin. | we have studied cefuroxime, a new beta-lactamase resistant cephalosporin, and cefoxitin, the first cephamycin antibiotic, which is also resistant to many beta-lactamases. both of these antibiotics have been shown to be microbiologically superior to the "first generation" cephalosporins, cefuroxime having notable activity against haemophilus influenzae, and cefoxitin against bacteroides fragilis. neither antibiotic is absorbed from the gut but, following parenteral administration, serum, urine an ... | 1978 | 308260 |
| beta-lactamases of subspecies of bacteroides fragilis. | | 1978 | 308502 |
| factors affecting antimicrobial agents in an anaerobic abscess. | | 1978 | 308504 |
| cefotoxime (hr 756) a new cephalosporin with exceptional broad-spectrum activity in vitro. | | 1978 | 308505 |
| activity of hr 756 against haemophilus influenzae, bacteroides fragilis and gram-negative rods. | | 1978 | 308506 |
| apparatus for uniaxial testing of soft human tissues in vitro. | | 1978 | 309051 |
| analysis of human lymphocyte subpopulations responsible for antibody-dependent and spontaneous cell-mediated cytotoxicity. | | 1979 | 313102 |
| [antibacterial activity of sulfobenzylpenicillin against anaerobic bacteria (author's transl)]. | | 1979 | 313459 |
| susceptibility to cefazedone and cefazolin and production of beta-lactamase in in bacteroides fragilis and other species of bacteroidaceae. | | 1979 | 314805 |
| ly-127935: a novel beta-lactam antibiotic with unusual antibacterial activity. | the in vitro activity of ly-127935, a new beta-lactam antibiotic, was examined by using 370 clinical bacterial strains. in comparison with several other beta-lactam agents, ly-127935 was the most inhibitory against the enterobacteriaceae. it was remarkably active against multi-drug-resistant strains of enterobacter spp., serratia spp., and pseudomonas aeruginosa. ly-127935 had four- to eightfold greater activity than did cefoxitin against bacteroides fragilis. production of beta-lactamase by ent ... | 1979 | 315753 |
| penetration of cephalothin and cefoxitin into experimental infections with bacteroides fragilis. | the in vitro activities of cephalothin and cefoxitin against bacteroides fragilis were studied by time-kill curves and measurement of residual drugs in culture supernatants. cefoxitin was bactericidal, causing a decrease of 10(7) in viable counts over 24 hr. cephalothin caused an initial decrease of 10(2) b. fragilis at 2 hr; this change was followed by growth of the organism within 24 hr back to the number present before addition of cephalothin. the concentration of cephalothin in broth decreas ... | 1979 | 318211 |
| cefoxitin therapy for bacterial endocarditis. | of 22 patients who were suspected of having bacterial endocarditis and who were treated with cefoxitin intravenously (8-12 g per day), 12 were evaluated for responses to therapy. ten patients had infections due to a single pathogen, and two had polymicrobial infections. staphylococci were isolated from eight patients, and streptococci from four; both of these pathogens were susceptible to 2-16 micrograms of cefoxitin/ml. staphylococcus aureus and four strains of anaerobic bacteria, including bac ... | 1979 | 318219 |
| the fetid foot: lower-extremity infections in patients with diabetes mellitus. | infections of the lower extremities in patients with diabetes mellitus have been attributed to staphylococcus aureus and other facultatively anaerobic bacteria. however, a review of 30 consecutive diabetics who required surgery for lower-extremity infections revealed that 17 had mixed infections due to both obligate and facultative anaerobes; only six had infections due to s. aureus. mixed infections often had characteristics of anaerobic suppuration and responded poorly to therapy. patients wit ... | 1979 | 318225 |
| in vitro activity of cefoxitin and parenterally administered cephalosporins against anaerobic bacteria. | the in vitro activity of cefoxitin against 221 recent isolates of anaerobic bacteria was compared with that of cefamandole and cefuroxime by the agar dilution technique. at achievable serum levels of 32 micrograms/ml, all three drugs were active against most groups of anaerobic bacteria tested. cefoxitin was active against most strains of the bacteroides fragilis group, whereas cefamandole and cefuroxime were relatively ineffective. cefoxitin was least active against clostridium species other th ... | 1979 | 318226 |
| clindamycin and gentamicin for aerobic and anaerobic sepsis. | thirty-eight adult patients with serious pleuropulmonary, soft-tissue, bone, and intra-abdominal infections caused by combinations of aerobic, facultative, and anaerobic bacteria were treated with parenterally given clindamycin phosphate and gentamicin sulfate and surgery when appropriate. nine had associated bacteremia. in 29, infections failed to respond to other therapeutic regimens, which included penicillins, cephalosporins, aminoglycosides, and chloramphenicol. results with clindamycin and ... | 1977 | 318824 |
| bactericidal activity of tinidazole. an in vitro comparison of the effects of tinidazole and metronidazole against bacteroides fragilis and other anaerobic bacteria. | the new antiprotozoal agent, tinidazole, was found to be bactericidal against all 52 isolates of obligate anaerobic bacteria tested, 42 bacteroides fragilis, 4 clostridia and 6 peptostreptococci. the minimum bactericidal concentrations of tinidazole for b. fragilis ranged from 0.25 to 4 mug/ml, and those of metronidazole from 0.25 to 8 mug/ml, i.e. several times lower than the serum concentrations achievable after oral administration. in most cases the mic was identical with mbc or half of it. o ... | 1977 | 318974 |
| development of a lysis-filtration blood culture technique. | a lysed-blood culture system that quickly lyses patients' blood near neutrality and is relatively noninjurious to more delicate pathogens such as haemophilus influenzae and bacteroides fragilis is reported. the lysing solution includes culture medium, 0.004 m sodium carbonate and bicarbonate, 0.04% triton x-100,and 0.6% rhozyme (a mixture of proteases). most of the pathogens tested multiplied in the lysing solution. the lysed blood normally is immediately filtered. the membrane is transferred to ... | 1977 | 319108 |