| hypertrophic gastropathy with transient sessile polyps. | we report an 8-year-old boy with hypertrophic gastropathy (hg) associated with duodenal giardia lamblia infestation. the follow-up was complicated by the development of gastric polyps at the site of previous biopsies that spontaneously disappeared within 15 months. despite the histological similarity, the different course between ménétrier's disease (md) in adults (chronic, with frequent development of sessile or pedunculate polyps) and hg (uncomplicated and usually spontaneously resolving) sugg ... | 1992 | 1619539 |
| helicobacter, helicobacter everywhere, and not a drop to drink! | | 1992 | 1619541 |
| protein-losing enteropathy and helicobacter pylori. | | 1992 | 1619544 |
| [peptic acid disease associated with helicobacter pylori in children. report of a case]. | we studied a child with peptic acid disease which was associated with h. pylori. the identification of h. pylori was done by biopsy and for antibody test. clinical improvement was observed only with the use of amoxocilin and bismuth subsalycilate. | 1992 | 1621037 |
| peptic ulcer disease. | peptic ulcer disease affects between 5 percent and 10 percent of americans during their lifetime. there are a variety of predisposing factors, including nonsteroidal anti-inflammatory drug (nsaid) use, helicobacter pylori infection, tobacco use and, possibly, certain dietary practices. while h2-receptor antagonists remain the mainstay of therapy, the selection of an antiulcer drug should be individualized. famotidine is preferred in persons who use alcohol, sucralfate may be preferred for mainte ... | 1992 | 1621633 |
| [development of genetic and molecular approaches for the diagnosis and the study of the pathogenic power of helicobacter pylori, agent of inflammatory gastric diseases]. | helicobacter pylori (h. pylori) is a small gram negative bacillus, recently discovered, found in the stomach of patients with active chronic gastritis and duodenal ulcers. production of a potent urease has been described as a trait common to all h. pylori so far isolated. to clarify the role of urease in the pathogenic process, as well as to engineer genetic tools useful for the diagnosis of h. pylori, we cloned the genes responsible for urease activity. a genomic library was constructed in esch ... | 1992 | 1622109 |
| chemotactic activity of helicobacter pylori sonicate for human polymorphonuclear leucocytes and monocytes. | the immunopathology of helicobacter pylori associated active chronic gastritis, which is characterised by predominance of polymorphonuclear leucocyte infiltration, is largely unknown. to evaluate the role of bacterial components as inflammatory mediators ultracentrifuged sonicated preparations were made of clinical isolates of helicobacter pylori. the crude sonicates were shown to exhibit chemotactic activity for human polymorphonuclear leucocytes and blood monocytes in a concentration dependent ... | 1992 | 1624151 |
| optimal survival of helicobacter pylori under various transport conditions. | the ability of clinical isolates and type strains of helicobacter pylori to survive in stuart transport medium, isotonic saline solution, and urea-containing isotonic saline was evaluated. the influences of temperature (4, 10, 15, 20, and 30 degrees c) and holding time (6 to 48 h) and the effect of exposure to air on survival were also studied. the recovery rate of h. pylori was highest from stuart transport medium in comparison with the recoveries from the other transport media tested. we found ... | 1992 | 1624562 |
| evaluation of the performance of commercial test kits for detection of helicobacter pylori antibodies in serum. | we have compared the sensitivities, specificities, and predictive values of three commercial serological assays for the diagnosis of helicobacter pylori infection. a qualitative latex method (pyloriset; orion diagnostics), a semiquantitative enzyme-linked immunosorbent assay (elisa) (gap test igg; bio-rad), and a quantiative elisa (helico-g; porton cambridge) were used in 109 untreated dyspeptic patients. the presence of h. pylori was established when the results of culture and/or histology of t ... | 1992 | 1624571 |
| expression of hla-dr antigen in different histological types of gastric polyp. | to study the expression of hla-dr antigen in the different histological types of gastric polyps. | 1992 | 1624598 |
| [helicobacter pylori spectrum: from gastritis to malignancy]. | | 1992 | 1625512 |
| [helicobacter pylori colonization of the gastric remnant following partial resection]. | colonization by helicobacter pylori (hp) in 53 patients after remote gastric surgery and roux-en-y anastomosis was investigated using a rapid urease test (cu-test) and histology (h&e and giemsa stain). the ph-value of gastric juice was measured. hp infection of the gastric remnant was found in 56% of 43 patients after partial resection and in no case after total gastric resection. hp-positive and hp-negative patients were comparable in age, sex distribution, indication and technique of gastric o ... | 1992 | 1626250 |
| immunoglobulin g antibodies to helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique. | helicobacter pylori is a gram-negative, curved, rod-shaped bacterium known to cause gastritis and to be an important factor in the pathogenesis of peptic ulcers. serological testing has recently been proposed as an aid in diagnosis of h. pylori infections. in this study, we used the western immunoblot technique to evaluate the possibility of using one or more of the antigens from h. pylori for this purpose. thirteen major bands and about 30 minor bands could be identified by western blotting whe ... | 1992 | 1629330 |
| helicobacter pylori has an ulcerogenic action in the ischemic stomach of rats. | helicobacter pylori (h. pylori) is now accepted as an important cause of chronic active gastritis. there also seems to be an association between the colonization of h. pylori in the gastric mucosa and peptic ulceration. however, it has not demonstrated that the instillation of h. pylori into the stomach produces the ulcerative gastric lesions in animals or humans. we carried out an experiment to study whether or not h. pylori has an ulcerogenic action in the ischemic stomach of rats, using an ex ... | 1992 | 1629566 |
| cytopathic effects of helicobacter pylori on cultured mammalian cells. | cytopathic effects of broth-culture filtrates from eight clinical isolates and one reference strain of helicobacter pylori on three cultured mammalian cell lines were investigated. all the strains, including nctc 11637, produced cytotoxic factors that caused intracellular vacuolation on these cell lines. ags and sflep cells were more sensitive than hep-2 cells. to examine the role of urease in the cytotoxic effect, a urease-negative mutant was produced. filtrates from both wild-type and mutant s ... | 1992 | 1629896 |
| motility as a factor in the colonisation of gnotobiotic piglets by helicobacter pylori. | non-motile variants of helicobacter pylori (strain 26695) occurred with a frequency of 1.6 (sd 0.4) x 10(-4) variants/cell/division cycle, and reversion to the motile form occurred with a frequency of less than 10(-7) variants/cell/division cycle. the two forms remained greater than 90% pure for up to 50 cell divisions and differed only in the presence or absence of motility and flagella. bacteria were recovered from nine of 10 gnotobiotic piglets inoculated orally with motile h. pylori, but fro ... | 1992 | 1629897 |
| helicobacter pylori infection in dialyzed patients: bismuth therapy and pharmacokinetics. | | 1992 | 1630551 |
| [stomach ulcer and helicobacter pylori]. | | 1992 | 1632003 |
| [helicobacter pylori]. | helicobacter pylori (hp) are gram-negative spiral bacteria which occur in the human stomach. the bacteria were cultured in vitro for the first time in 1983. it is suspected that the bacteria may cause chronic gastritis of type b and may also be a contributory cause of chronic ulceration and cancer of the stomach. the bacteria are accompanied by characteristic inflammatory changes in the gastric mucosa. the significance for gastritis, chronic ulceration, non-ulcer dyspepsia and carcinoma of the s ... | 1992 | 1632004 |
| inhibition of helicobacter pylori glycosulfatase activity toward gastric sulfomucin by nitecapone. | a glycosulfatase activity toward gastric sulfomucin was identified in the extracellular material elaborated by h. pylori. the enzyme exhibited maximum activity at ph 5.7 in the presence of triton x-100 and cacl2, and displayed on sds-page an apparent molecular weight of 30kda. the h. pylori glycosulfatase effectively caused desulfation of n-acetylglucosamine-6-sulfate and galactose-6-sulfate of the carbohydrate chains of mucins, as well as that of glucose-6-sulfate of glyceroglucolipids, but was ... | 1992 | 1632804 |
| [clinical significance of serological markers of helicobacter pylori]. | helicobacter pylori (h. pylori) is a gram-negative, curved bacillus known since 1983. it is supposed to play role in the pathogenesis of certain gastroduodenic diseases, e.g. non-ulcer dyspepsia (nud), chronic inflammation, ulcers, etc. serum samples of 70 patients who were examined for stomach complaints with gastroscopy and those of 22 healthy persons were analysed. the purpose of the study was to evaluate anti-h. pylori igg, igm and iga antibodies using elisa method. whether the antibodies ca ... | 1992 | 1635774 |
| [the non-ulcer dyspepsia syndrome]. | | 1992 | 1635780 |
| ebrotidine effect on the proteolytic and lipolytic activities of helicobacter pylori. | 1. the effect of ebrotidine, a new antiulcer agent, on the activity of mucus degrading of protease and lipase enzymes elaborated by helicobacter pylori was investigated. 2. in the absence of ebrotidine, the h. pylori protease caused extensive degradation of gastric mucus protein, while free fatty acids, glycerol mono-oleate and lysophosphatidylcholine were produced by the action of h. pylori lipase and phospholipase a enzymes. 3. introduction of ebrotidine to the incubation system led to the red ... | 1992 | 1639232 |
| eicosanoid synthesis and helicobacter pylori associated gastritis: increase in leukotriene c4 generation associated with h. pylori colonization. | the importance of pro-inflammatory leukotriene c4 in helicobacter pylori (h. pylori) associated gastritis in man is unknown. fresh gastric biopsy specimens from 28 dyspeptic patients were obtained: 10 showed normal antral histology with no evidence of h. pylori, the remaining 18 patients exhibited histological gastritis and were h. pylori positive as assessed by histology, culture and urease test. twelve of these 18 patients received 240 mg twice daily colloidal bismuth subcitrate for four weeks ... | 1992 | 1641505 |
| serodiagnosis of helicobacter pylori-associated gastritis with a monoclonal antibody competitive enzyme-linked immunosorbent assay. | forty-nine monoclonal antibodies against helicobacter pylori were screened to investigate their capacity to be used in enzyme-linked immunosorbent assay (elisa) competitive systems for the serodiagnosis of helicobacter pylori infection. on the basis of the inhibition pattern showed by the sera of five infected patients, the antibodies were subdivided into five groups. the immunoblotting analysis showed that the antibodies recognized a total of nine different antigenic determinants. in a study of ... | 1992 | 1641587 |
| [helicobacter pylori infections in children]. | in our patient population with chronic aspecific abdominal complaints helicobacter pylori gastritis is no rarity. this entity has been diagnosed in 10 children out of 48 (mean age: 8 years 11 months) who underwent an endoscopy of the upper gastrointestinal tract. after treatment with amoxicillin and colloidal bismuth subcitrate during a period of 2 weeks the complaints disappeared and an eradication was obtained in 7. histology of the antral mucosa demonstrated a type b chronic active gastritis ... | 1992 | 1641843 |
| visual endoscopic indicator of helicobacter pylori infection. | | 1992 | 1642216 |
| a prospective randomized study of the injurious effects of aspirin and naproxen on the gastroduodenal mucosa in patients with rheumatoid arthritis. | a prospective double-blind randomized placebo-controlled study was conducted to compare the injurious effects of aspirin and naproxen on the gastroduodenal mucosa. patients with rheumatoid arthritis attending the immunology clinic of a tertiary care center were included in the study. ninety consecutive patients were randomized to receive aspirin, naproxen, or placebo. clinical evaluation was done every 2 wk. upper gastrointestinal endoscopy, biopsies from the gastric antrum and duodenum for hist ... | 1992 | 1642224 |
| helicobacter pylori infection in children. a clinical overview. | | 1992 | 1643767 |
| non-immunological defence mechanisms of the gut. | non-immunological defence mechanisms represent an important line of intestinal defence in addition to humoral and cellular immunity. this review summarises the evidence for the role of the non-immunological defence system. protective factors that have been amply documented are gastric juice, intestinal motility, and intestinal flora. components of pancreatic juice, lysozyme, and epithelial cell turnover may also be involved. special attention is given to gastric acid, infection with helicobacter ... | 1992 | 1644343 |
| [what do we know and what do we not know about peptic ulcer]. | | 1991 | 1654011 |
| [anti-helicobacter pylori drugs must be considered as first line therapy in peptic ulcer]. | | 1991 | 1658869 |
| role of cytokines in infectious and noninfectious enteropathy in patients with aids. | | 1991 | 1659606 |
| helicobacter pylori-associated gastritis is rare in aids: antibiotic effect or a consequence of immunodeficiency? | helicobacter pylori is the principal cause of type b histologic gastritis. aids is associated with increased susceptibility to gram-negative enteric infections. using a retrospective study design, we have determined the prevalence of h. pylori-associated histologic gastritis (based on gastric histopathology) in 201 patients with aids. these data were compared with h. pylori prevalence rates (based on serology) from healthy hiv-negative, age-matched australian controls (n = 785) and a cohort (n = ... | 1991 | 1660218 |
| [elisa for detection of anti-urease antibodies of campylobacter pylori and its application]. | the urease extracted from 6 strains of helicobacter pylori (hp) was used as antigen to detect sera anti-urease antibodies of hp in 136 patients with gastric diseases and 13 persons with normal gastric mucosa by enzyme-linked immunosorbent assay (elisa). the antigen proved to be pure and specific for elisa. for a titre of 450eu or over 450eu the test had a sensitivity of 91.96% and a specificity of 86.49%. the results of the elisa showed excellent correlation with microbiological detection of hp ... | 1991 | 1660762 |
| azithromycin levels in plasma and gastric tissue, juice and mucus. | azithromycin is the first member of a new class called the azalides. its distribution in gastric tissues was studied in 27 patients (mean age 66 years) with proven gastric cancer due to be resected. five groups of patients received a single 500 mg oral dose of azithromycin 24, 48, 72, 96 or 120 h pre-operatively. samples of blood, gastric juice, mucus and gastric tissue were taken and azithromycin assayed by high performance liquid chromatography. there was an increase in the level of azithromyc ... | 1991 | 1662634 |
| inhibition of human gastric cyclic amp production by helicobacter pylori protein--possible involvement of mucosal prostaglandin e2. | the effect of helicobacter pylori protein on camp and prostaglandin (pge2) production was studied in incubates of human gastric fundic mucosa. at 24 hours, specimens incubated in the control fluid had a median camp value of 81 pmol/mg protein, compared to 28 pmol/mg (p less than 0.05) when incubated in h. pylori protein, 155 pmol/kg (p less than 0.006) in histamine, and 23 pmol/kg (p less than 0.05) in histamine plus h. pylori protein. a similar trend was observed at 48 hours. although h. pylori ... | 1991 | 1663793 |
| [chronic gastritis and helicobacter pylori infection on the ivory coast. a series of 277 symptomatic patients]. | only a few reports have established the importance of chronic gastritis and helicobacter pylori infection in africa. the aim of this study was to ascertain the prevalence of chronic gastritis and helicobacter pylori infection in a symptomatic population in abidjan, côte-d'ivoire. the study included 277 consecutive patients referred for gastroscopy. two hundred nine patients complained of abdominal pain without gastroduodenal ulcer or cancer: 26 had a duodenal ulcer, 23 had a gastric ulcer, and 6 ... | 1991 | 1667767 |
| [preliminary study of the prevalence of helicobacter pylori in tananarive, madagascar and the antibacterial activity in vitro of 13 malagasy medicinal plants on this germ]. | helicobacter pylori infection has been studied in 60 patients who were suffering from either a peptic ulcer disease or a gastritis complaint. helicobacter pylori was searched in antral biopsy specimens using standard bacteriological methods (gram-staining urea-test, culture) and histological staining techniques (hematoxylin-eosin-saffron, undifferentiated giemsa). the culture was successful in 40 cases. the antibacterial activity of 13 malagasy medicinal plants has been tested versus helicobacte ... | 1991 | 1669364 |
| anti-helicobacter pylori therapy: clearance, elimination, or eradication? | | 1991 | 1671157 |
| follow-up after anti-helicobacter pylori treatment. | | 1991 | 1671932 |
| helicobacter pylori infection in cohabiting children. | | 1991 | 1672930 |
| lack of eradication of helicobacter pylori after omeprazole. | | 1991 | 1673511 |
| [prevention of duodenal ulcer recurrence. a new prospect]. | prevention of recurrence is now the main therapeutic problem in the management of duodenal ulcer disease. it has been shown that in the absence of maintenance treatment the mean recurrence rate within 6 to 12 months from healing obtained with drugs other than h2-receptor antagonists (h2a) is lower than when healing is obtained with h2a. possible reasons for this discrepancy are examined. when the recurrence rates observed with each of the non-h2a drugs were compared individually with those obser ... | 1991 | 1673786 |
| achlorhydria-induced hypergastrinaemia: the role of bacteria. | 1. studies of helicobacter pylori show that microbes can alter gastrin release. lack of gastric acid (achlorhydria) causes hypergastrinaemia and allows bacteria to grow within the stomach. we speculated that the bacteria contribute to the rise in gastrin seen after acid inhibition, and tested the idea by comparing plasma gastrin levels during inhibition of acid secretion between germ-free and conventional rats. 2. matched germ-free and conventional rats (n = 8 per group) received either vehicle ... | 1991 | 1673881 |
| frozen biopsy specimens and culture rates of helicobacter pylori. | | 1991 | 1674782 |
| activity of lansoprazole against helicobacter pylori. | | 1991 | 1675355 |
| water source as risk factor for helicobacter pylori infection in peruvian children. gastrointestinal physiology working group. | helicobacter pylori infection is widespread among peruvian adults by the age of 30, but the age at which children become infected, the prevalence of disease, and the role of socioeconomic status in the epidemiology of infection are not known. we used the 13c-urea breath test to study the prevalence of infection in 407 peruvian children from lima, aged 2 months to 12 years, from families of low and high socioeconomic status. peruvian children acquire h pylori early in life and the number of infec ... | 1991 | 1675369 |
| duodenal ulcer relapse after eradication of helicobacter pylori. | | 1991 | 1675746 |
| screening dyspepsia by serology to helicobacter pylori. | owing to limited endoscopy resources, various screening strategies for endoscopy have been proposed. helicobacter pylori can be detected with high sensitivity and specificity by serology, and therefore we assessed the effects on diagnostic accuracy and endoscopic workload of a policy of screening clinic patients with dyspepsia before endoscopy by a strategy based on age, helicobacter pylori serology, and use of non-steroidal anti-inflammatory drugs. 1153 patients were studied, of whom 842 were o ... | 1991 | 1676478 |
| much ado about gastrin. | the regulation of gastrin secretion from antral g-cells is of major importance in the physiologic control of acid secretion. gastrin secretion is highly dependent upon gastric intraluminal ph and is inhibited significantly by a ph of less than 3.0. acute gastric alkalinization greater than ph 6.0 with antisecretory agents such as h2-receptor antagonists or h+/k+ atpase inhibitors has little impact on fasting serum gastrin levels but promotes an enhanced sustained rise in meal-stimulated gastrin ... | 1991 | 1676713 |
| isolation of helicobacter pylori from frozen gastric biopsy specimens. | | 1991 | 1676805 |
| an increased number of gamma/delta t-cells and gastric epithelial cell expression of the groel stress-protein homologue in helicobacter pylori-associated chronic gastritis of the antrum. | numerous studies have shown that the presence of helicobacter pylori in the stomach is linked to the development of chronic gastritis most commonly seen in the antrum. however, the pathogenic mechanisms are unclear. in 23 of 31 patients, examined due to symptoms from the upper gastrointestinal tract, h. pylori-associated chronic gastritis of the antrum was diagnosed histologically and by growth of h. pylori. immunoperoxidase staining on gastric biopsy specimens from these patients showed an incr ... | 1991 | 1677527 |
| mucosal iga recognition of helicobacter pylori 120 kda protein, peptic ulceration, and gastric pathology. | the gastric iga response to helicobacter pylori was examined in 100 dyspeptic patients by means of immunoblotting of supernatants from antral biopsy and gastric mononuclear cell cultures. 76 of 78 patients with chronic gastritis, 2 of 8 with reactive gastritis, and 1 of 14 subjects with normal mucosa showed positive responses. of patients with chronic gastritis, 75%, 83%, 97% and 76%, respectively, showed responses to the 120 kda, 90 kda, 61 kda, and 31 kda proteins. none of the 19 patients with ... | 1991 | 1677696 |
| screening for helicobacter pylori in dyspepsia. | | 1991 | 1677739 |
| second annual symposium on helicobacter pylori. cincinnati, ohio, usa, 6 and 7 august 1990. | | 1991 | 1678205 |
| duodenal ulcer recurrence and helicobacter pylori. | | 1991 | 1678818 |
| screening dyspepsia by serology to helicobacter pylori in children. | | 1991 | 1681395 |
| symposium on helicobacter pylori: a cause of gastroduodenal disease. cincinnati, ohio, 9-10 october 1989. | | 1991 | 1681582 |
| screening for helicobacter pylori. | | 1991 | 1682570 |
| helicobacter pylori-associated gastritis and primary b-cell gastric lymphoma. | although lymphoid tissue is absent in normal gastric mucosa, primary lymphomas arise in the stomach and most of these recapitulate the features of mucosa-associated lymphoid tissue (malt). gastric lymphoid tissue is known to be acquired in response to local infection by helicobacter pylori, and we have confirmed this in 450 patients with h pylori-associated gastritis of whom 125 showed mucosal lymphoid follicles. in 8 patients, b lymphocytes infiltrated epithelium, which is a feature characteris ... | 1991 | 1682595 |
| one week eradication regimen for helicobacter pylori. | although helicobacter pylori is both a frequent cause of gastritis and an important factor in duodenal ulcer recurrence, no treatment regimen exists that is completely safe and effective. we have studied a short eradication regimen of tripotassium dicitrato bismuthate 120 mg four times daily and amoxycillin 500 mg four times daily for seven days with metronidazole 400 mg five times daily for three days (days 5-7). 106 patients with peptic ulceration and non-ulcer dyspepsia, who were also infecte ... | 1991 | 1682653 |
| treatment of duodenal ulcer with sucralfate. | sucralfate attains a healing rate of about 79% for duodenal ulcer in 4 weeks, which is similar to the effects of cimetidine and ranitidine. whereas cigarette smoking significantly affects duodenal ulcer healing by acid-reducing agents, the healing rates of smokers and non-smokers treated with sucralfate or colloidal bismuth are indistinguishable, suggesting an inherent advantage through the cytoprotective mechanisms of these agents. the 12-month relapse curves for duodenal ulcers initially heale ... | 1991 | 1683491 |
| helicobacter-associated duodenitis and gastric metaplasia in duodenal ulcer patients. | biopsy specimens were taken from the duodenal bulb and the distal duodenum in 45 duodenal ulcer patients before and after treatment with histamine-2 antagonists, prostaglandin analogues or antacids. after four weeks of treatment, the ulcer had healed in 31 patients. the treatment did not lead to a reduced frequency of helicobacter-associated duodenitis or gastric metaplasia of the duodenal epithelium. we found gastric metaplasia in 52.3% of all biopsy specimens from the duodenal bulb, chronic ac ... | 1991 | 1683540 |
| iv workshop on gastroduodenal pathology and helicobacter pylori. bologna, november 28-30, 1991. abstracts. | | 1991 | 1684305 |
| restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant campylobacter species. | whole-cell chromosomal digests of 84 strains of aerotolerant campylobacter (ac) were examined by using pvuii restriction fragment length polymorphisms of rrna genes followed by hybridization with escherichia coli 16s and 23s rrna (ribotyping). the ac strains belonged to campylobacter cryaerophila (n = 13) and a newly defined species, "c. butzleri" (n = 64). strains of c. cryaerophila belonged to two hybridization groups: dna group 1a (including the type strain of c. cryaerophila) and dna group 1 ... | 1991 | 1684797 |
| restriction fragment length polymorphism of helicobacter pylori using the urease gene probe. | | 1991 | 1684943 |
| mechanisms of injury and sequelae of helicobacter pylori infection. international workshop at deerhurst, ontario, canada, 21-24 february 1991. | | 1991 | 1685597 |
| evaluation of fingerprinting methods for identification of helicobacter pylori strains. | variation amongst strains of helicobacter pylori was examined by 35s-methionine-labelled protein sds-page (radio-page), immunoblot and dna fingerprinting techniques. these methods allowed discrimination amongst isolates and showed total correlation. pig and baboon gastric helicobacter pylori-like organisms were found to be very similar to helicobacter pylori by both radio-page and immunoblotting, whereas a helicobacter mustelae isolate was markedly different. the hindiii restriction endonuclease ... | 1991 | 1687029 |
| comparison of four stains and a urease test for rapid detection of helicobacter pylori in gastric biopsies. | gastric biopsies were obtained from 125 subjects to compare detection of helicobacter pylori by culture, a rapid urease test and histopathologic examination using haematoxylin-eosin, gram, giemsa, warthin-starry silver and acridine orange stains. helicobacter pylori was isolated from 39 specimens. acridine orange and giemsa were the most sensitive stains, detecting 85% and 79% of positive specimens respectively. all stains showed high specificity (97-100%). the sensitivity and specificity of the ... | 1990 | 1695573 |
| comparison of culture and histology for the identification of helicobacter pylori in endoscopic biopsies. | the aim of the study was to compare culture with microscopic examination of haematoxylin and eosin stained tissue sections in the identification of helicobacter pylori in gastric antral mucosa. duplicate gastric antral biopsies from 89 patients were cultured and examined histologically for h pylori. h pylori was cultured in 46 cases (51%) and identified histologically in 63 (71%). results from the two tests concurred in 63 cases (71%). the presence of h pylori was strongly correlated with histol ... | 1990 | 1697451 |
| use of dna restriction endonuclease digest and ribosomal rna gene probe patterns to fingerprint helicobacter pylori and helicobacter mustelae isolated from human and animal hosts. | variation amongst strains of helicobacter pylori and helicobacter mustelae was examined by dna restriction endonuclease digestion and rrna gene patterns generated using a non-radioactive probe. chromosomal dna was extracted from 30 cultures of h. pylori from human, rhesus monkey and pig gastric mucosa, and from three h. mustelae isolates from ferret gastric mucosa. dna fingerprinting with hae iii and hind iii showed h. mustelae was relatively homogeneous but revealed genomic heterogeneity within ... | 1990 | 1698260 |
| detection of antibodies to helicobacter pylori cell surface antigens. | serum igg antibodies of helicobacter pylori were detected in single-dilution elisa using glycine extracted material. among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90%) was found in patients with positive gastric culture for h. pylori than in culture negative patients (37%). among 68 blood donors the frequency of h. pylori antibodies was 28%. in 73 children less than 15 years of age examined for unrelated disorders the occurrence was 4%. by immunoblottin ... | 1990 | 1699265 |
| a spiral microorganism in the stomach of pigs. | light and electron microscopic and microbiologic evaluations were performed on mucosa of stomachs from 120 healthy slaughtered pigs. helicobacter pylori was not found, but a tightly spiralled bacterium, not previously described, was seen in histological sections and/or in carbol fuchsin stained smears in 13 (10.8%) stomachs. in paraffin sections stained with carbol fuchsin, the bacteria were seen in the mucus of the lumen of the antral pits and in the mucosa surface within and beneath the mucus. ... | 1990 | 1700535 |
| comparison of sensitivity and specificity of three different methods for the detection of helicobacter pylori infection. | | 1990 | 1701286 |
| superior stain for helicobacter pylori using toluidine o. | | 1990 | 1702103 |
| different effect of helicobacter pylori on the human gastric antral and body mucosal intracellular mucin. | to elucidate the role of helicobacter pylori infection in the pathogenesis of gastric ulcer, we investigated the intracellular mucin content by measuring the periodic acid-schiff-alcian blue (pas-ab)-stained substances, by means of computer, in the biopsy sample of gastric mucosa from patients with and without h. pylori infection. in the antral mucosa the intracellular pas-ab-stained mucin content was significantly smaller in patients with infection than in patients without infection, whereas in ... | 1990 | 1702231 |
| direct gram stain and urease test to detect helicobacter pylori. | antral biopsies were obtained by gastrointestinal endoscopy on 143 adult patients with dyspeptic symptoms of gastritis or peptic ulcer disease. a direct gram stain and a direct urease test were performed on each biopsy in addition to culture. forty-three biopsies (30%) were considered positive for helicobacter pylori based on culture or histologic examination, or both. thirty-one biopsies (72% sensitivity) were positive for both direct tests, whereas 95 of 100 negative cultures were negative for ... | 1990 | 1703938 |
| direct detection and amplification of helicobacter pylori ribosomal 16s gene segments from gastric endoscopic biopsies. | helicobacter pylori is an organism thought to play an important causative role in gastritis and peptic ulcer diseases. we have designed an rna dot blot assay for the detection of h. pylori, using as probe a synthetic oligonucleotide complementary to its 16s rrna. we have also used oligonucleotide primers, complementary to conserved sequences within bacterial ribosomal 16s genes, to amplify a h. pylori ribosomal 16s dna fragment via the polymerase chain reaction (pcr). after determining the dna s ... | 1990 | 1703940 |
| identification, characterization, and spatial localization of two flagellin species in helicobacter pylori flagella. | flagellar filaments were isolated from helicobacter pylori by shearing, and flagellar proteins were further purified by a variety of techniques, including cscl density gradient ultracentrifugation, ph 2.0 acid disassociation-neutral ph reassociation, and differential ultracentrifugation followed by molecular sieving with a sephacryl s-500 column or mono q anion-exchange column, and purified to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer to an ... | 1991 | 1704004 |
| fine structure of active and healed duodenal ulcer. | in order to characterize the fine structure of active and healed duodenal ulcers, we examined tissue specimens of patients with active duodenal ulcer disease (n = 30) before and after treatment with either antacids (n = 16) or h2-receptor antagonists (n = 14), by light microscopy and various electron microscopic techniques, e.g., scanning and transmission electron microscopy. the characteristic histological feature of both the active and healed duodenal ulcer was the appearance of periodic acid- ... | 1991 | 1704181 |
| [the determination of c. pylori in patients with gastroduodenal diseases]. | a total of 46 gastric mucosa biopsy specimens obtained during surgery and 90 gastric juice samples from patients with gastroduodenal diseases were examined. campylobacter-like microorganisms were detected in 84.6 percent of biopsy specimens and in 73.9 percent of gastric juice samples (in gram-stained smears). urease activity was detected in 75.9 and 13.3 percent, respectively. c. pylori growth in solid nutrient medium in microaerophilic conditions was observed in 44.2 and 5.7 percent of the exa ... | 1990 | 1704468 |
| phylogeny of helicobacter felis sp. nov., helicobacter mustelae, and related bacteria. | strain cs1t (t = type strain) is a gram-negative, microaerophilic, urease-positive, spiral-shaped bacterium that was isolated from the gastric mucosa of a cat. additional strains which possessed biochemical and ultrastructural characteristics similar to those of strain cs1t were isolated from the gastric mucosa of cats and dogs. the guanine-plus-cytosine content of the dna of strain cs1t was 42.5 mol%. the 16s rrna sequences of strain cs1t, strain ds3 (a spiral-shaped isolate from a dog), and he ... | 1991 | 1704791 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| characterisation of the urease from helicobacter pylori and comparison with the ureases from related spiral gastric bacteria. | the urease enzyme of helicobacter pylori was partially purified from whole cell extracts and found to have a molecular weight of 484 +/- 12 kda. ten monoclonal antibodies (mabs) were produced against four different epitopes of the native enzyme. these mabs also recognised the ureases of h. pylori-like organisms isolated from monkeys and pigs and the h. mustelae urease from ferrets. the urease enzymes of each of these organisms were found to be of the same molecular weight. the urease enzyme of h ... | 1991 | 1711875 |
| the usefulness of the acridine-orange stain in identifying helicobacter pylori in gastric biopsies. | the efficacy of the acridine-orange stain (aos) in identifying helicobacter pylori (hp)-like organisms in biopsy smears from adults with gastroduodenal disease was studied. the results obtained indicate that aos can replace gram stain in hp organism identification in gastroduodenal mucosa specimen. | 1991 | 1713287 |
| [helicobacter pylori--a pathogen with many faces]. | | 1991 | 1714173 |
| [virulence factors of helicobacter pylori and pathomechanisms of mucosal damage]. | | 1991 | 1714174 |
| [effect of helicobacter pylori on stomach physiology]. | | 1991 | 1714175 |
| [helicobacter pylori infection: a facilitator of stomach cancer?]. | | 1991 | 1714176 |
| [epidemiology and diagnosis of helicobacter pylori infection]. | | 1991 | 1714177 |
| [helicobacter pylori infection, gastritis and non-ulcer dyspepsia--is there a connection?]. | | 1991 | 1714178 |
| [methods of isolating and identifying campylobacter pylori (review of the literature)]. | | 1991 | 1714997 |
| rapid diagnosis of campylobacter pylori infection by urea test paper. | antral biopsy specimens from 106 patients were examined by culture, gram stain and silver stain for campylobacter pylori. biopsies were also examined by a urea test paper test (utpt). of 106 patients studied c. pylori was detected in 68 (64.2%) by gram stain, silver stain and culture. the utpt was positive in 63 (59.4%) specimens. five had false negative results using the utpt with no false positive subjects. thus, utpt has a sensitivity of 92.6% (63/68) and a specificity of 100%. of the 63 spec ... | 1990 | 1717032 |
| detection of helicobacter pylori in stomach tissue by use of a monoclonal antibody. | monoclonal antibodies were produced against an acid glycine extract of helicobacter pylori atcc 43504t. one of these appeared to be specific for h. pylori; it recognized all h. pylori isolates by an indirect immunofluorescence assay (iif) but it did not cross-react with the other strains tested, including different species of the genera helicobacter, campylobacter, and wolinella. different strains of members of the families enterobacteriaceae and pseudomonadaceae or other gram-negative bacteria ... | 1991 | 1721923 |
| a high frequency of detection of helicobacter pylori in whitish exudate of gastric ulcer. | helicobacter pylori has been implicated in the pathogenesis of chronic gastritis and peptic ulcer. however, no report to date has been made on the presence of h. pylori in the whitish exudate of peptic ulcers. we examined the biopsy specimens of 52 patients (42 men and 10 women, aged 29-78, not taking steroids or nonsteroid antiinflammatory drugs), and evaluated the frequency of detecting the organism in the whitish exudate of gastric ulcers in comparison with that in the ulcer border, gastric f ... | 1991 | 1722231 |
| [a rapid indication of helicobacter pylori in patients with gastroduodenal pathology]. | rapid detection of h. pylori in samples from gastroduodenal patients is based on the use of standard ingredients made in the ussr: disks with urea from kits and indicator strips to show ph values of the rifan type, within the ph range 5.8-7.4. this method helps detect ph changes emerging in h. pylori urea disintegration in the liquid and gaseous (air) phases. the sensitivity, specificity, and rate of the test are not inferior to those of the commercial urease test. | 1991 | 1722536 |
| direct polymerase chain reaction test for detection of helicobacter pylori in humans and animals. | we designed a polymerase chain reaction (pcr) for amplifying the helicobacter pylori gene encoding 16s rrna. primers for the specific detection of h. pylori were designed for areas of the 16s rrna gene in which there is the least sequence homology between h. pylori and its closest relatives. the specificity of detection was confirmed by ensuring that the primers did not amplify dna extracts from the campylobacters h. cinaedi, h. mustelae, and wolinella succinogenes, which are the closest relativ ... | 1991 | 1723072 |
| [determination of the degree of seeding by helicobacter pylori in the gastric mucosa]. | | 1991 | 1724047 |
| [histologic study of helicobacter pylori in 265 consecutive gastric biopsies]. | we evaluated 265 consecutive endoscopic gastric biopsies of subjects attending the gastric cancer mass survey that is taken place in the state of tachira, venezuela. they were 152 males and 113 females between 18 and 70 years of age. we established the prevalence of helicobacter pylori in relation with histologic diagnosis and location of the lesions in the stomach. we performed hematoxylin eosin, giemsa and gimenez stains. the bacteria was identified in chronic non atrophic gastritis in 81% of ... | 1991 | 1726912 |