| pulmonary ischemia/reperfusion injury: a quantitative study of structure and function in isolated heart-lungs of the rat. | early graft dysfunction after lung transplantation is a significant and unpredictable problem. our study aimed at a detailed investigation of structure-function correlations in a rat isolated heart-lung model ofischemia/ reperfusion injury. variable degrees of injury were induced by preservation with potassium-modified euro-collins solutions, 2 hr of cold ischemia, and 40 min of reperfusion. pulmonary artery pressure (ppa), pulmonary vascular resistance (pvr), peak inspiratory pressure (pip), an ... | 1999 | 10321996 |
| reduced neutrophil infiltration protects against lung reperfusion injury after transplantation. | there is evidence that lung ischemia reperfusion injury is a result of the activation of components of the inflammatory cascade. however, the role of neutrophils in lung reperfusion injury continues to be a source of controversy. | 1999 | 10355425 |
| effects of lung preservation solutions on pmn activation in vitro. | polymorphonuclear leukocyte (pmn) activation and pmn-endothelial cell interactions may cause graft failure due to ischemia-reperfusion injury after lung transplantation. we investigated the effects of euro-collins solution (ec), low-potassium dextran solution (lpd), and ec plus pentoxifylline (ec-ptxf) on adhesion molecule (cd11b/cd18 and l-selectin) expression, chemotaxis, and oxidative burst of pmn. pmn from healthy human volunteers were incubated with ec, lpd, and ec-ptxf, and, in controls, w ... | 1999 | 10363593 |
| influence of different routes of flush perfusion on the distribution of lung preservation solutions in parenchyma and airways. | the present study was performed to investigate the influence of different routes of perfusion on the distribution of the preservation solutions in the lung parenchyma and upper airways. | 1999 | 10371126 |
| endothelium-dependent relaxation of canine pulmonary artery endothelium after prolonged preservation. | experiments were designed to investigate the effect of euro-collins (ec) solution and university of wisconsin (uw) solution on function of pulmonary arterial endothelium. | 1998 | 10374397 |
| relationships between cytochrome oxidase (co) blobs in primate primary visual cortex (v1) and the distribution of neurons projecting to the middle temporal area (mt). | the cytochrome oxidase (co) blobs and interblobs in layer 3b of primate visual cortex have different sets of corticocortical connections. cortical layers below layer 3b also project corticocortically, but the relationship of efferent projections from the deeper layers to the overlying blob/interblob architecture is less clear. we studied the tangential organization of neurons projecting from primary visual cortex (v1) to the middle temporal visual area (mt) and their relationship to the co blobs ... | 1999 | 10376741 |
| enhancement of hypothermic heart preservation with fructose 1, 6-diphosphate. | we hypothesized that the addition of fructose 1, 6-diphosphate (fdp) to a hypothermic heart preservation solution could improve metabolic recovery because it has several beneficial effects. | 1999 | 10383848 |
| superior protection in orthotopic rat lung transplantation with cyclic adenosine monophosphate and nitroglycerin-containing preservation solution. | primary lung graft failure is common, and current lung preservation strategies are suboptimal. because the decline in lung levels of cyclic adenosine monophosphate and cyclic guanosine monophosphate during preservation could enhance adhesiveness of endothelial cells for leukocytes as well as increase vascular permeability and vasoconstriction, we hypothesized that buttressing these levels by means of a preservation solution would significantly improve lung preservation. | 1999 | 10384196 |
| celsior solution for improvement of currently used clinical standards of lung preservation in an ex vivo rat model. | the introduction of euro-collins solution with its intracellular electrolyte composition has allowed for clinically accepted pulmonary preservation for up to 7 h of ischemic time. in recent years several alternative solutions have been developed for the improvement of pulmonary preservation. celsior is an extracellular solution which has significantly reduced the ischemia/reperfusion (ir)-induced pulmonary damage in animal studies. so far, no larger experimental studies exist concerning the infl ... | 1999 | 10386415 |
| the historical roots of high rates of infant death in aboriginal communities in canada in the early twentieth century: the case of fisher river, manitoba. | infant mortality is investigated for a period of thirty years at the beginning of the 20th century in the aboriginal nations community of fisher river, manitoba. infant mortality rates were generated from parish records of infant burials from the methodist mission at fisher river and later archived at the united church archives in winnipeg, man. the average infant mortality rate (imr) for the total period (1910-1939) was 249 per 1000 live births, an exceedingly high rate compared to modern imrs ... | 1999 | 10405019 |
| investigation of possible participation of nucleoside transport systems in the postischemic release of purines and pyrimidines from cold stored liver. | the aim of the study was to elucidate the role of nucleoside transport systems in the postischemic release of nucleosides and nucleobases accumulated by the rat liver during cold storage. livers were preserved for 24 h in euro-collins (ec) or in a lactobionate-based solution (lbs) without exogenous adenosine. the rates of release of uric acid, xanthine, hypoxanthine, inosine, adenosine, uridine, and cytidine were monitored during early reperfusion. the greater part of the purines and pyrimidines ... | 1999 | 10413569 |
| beneficial effects of leukocyte-depleted blood and low-potassium dextran solutions on microvascular permeability in preserved porcine lung. | modified euro-collins (ec) solution, a crystalloid intracellular-type solution, has been commonly used for pulmonary preservation. several experimental studies have shown the advantages of using extracellular colloid-based solutions. the aim of this study was to compare the quality of preservation of two extracellular colloid solutions, leukocyte-depleted blood (bl) and low-potassium dextran (lpd) solutions, with that of ec solution. lungs of 22 domestic pigs were flushed and preserved with ec ( ... | 1999 | 10430747 |
| ultrastructural alterations in intraalveolar surfactant subtypes after experimental ischemia and reperfusion. | ischemia and reperfusion (i/r) result in surfactant dysfunction. whether the impairment of surfactant is a consequence or a cause of intraalveolar edema formation is still unknown. the cumulative effects of lung perfusion, ischemic storage, and subsequent reperfusion on surfactant ultrastructure and pulmonary function were studied in a rat isolated perfused lung model. the left lungs were fixed for electron microscopy by vascular perfusion either immediately after excision (control; n = 5) or af ... | 1999 | 10430751 |
| ethnic affinities of the ancient human jety-asar population by mitochondrial dna analysis. | an anthropological study of the remains has indicated uniformity of the ancient human jety-asar population (central asia) and suggests a mixed euro-mongoloid genesis. dna was extracted from teeth from three caucasoid skulls recovered from a burial site dated at approximately 2000 years ago. ancient mitochondrial dna (mtdna) was analysed by restriction fragment length polymorphism (rflp) analysis for the a, b, c and d haplogroups and the sequencing of hypervariable region i of the mtdna control r ... | 1999 | 10435439 |
| renoprotective effects of trimetazidine against ischemia-reperfusion injury and cold storage preservation: a preliminary study. | initial ischemia-reperfusion injury is associated with organ retrieval, storage, and transplantation adversely affects early graft function and influences the development of chronic graft dysfunction. we have recently shown that the protective agent trimetazidine (tmz) added to preservation solutions: euro-collins (ec) and university of wisconsin (uw) was efficient to protect kidneys from ischemia-reperfusion injury in an isolated perfused kidney model. we extended these observations to investig ... | 1999 | 10440406 |
| osmotic tolerance limits of canine pancreatic islets. | future improvements in the recovery and function of pancreatic islets following cryopreservation will require a more precise quantification of the stresses that occur at each stage of the cryopreservation protocol. changes in solution osmolality during the addition and dilution of cryoprotectants and during freezing and thawing induce changes in islet volume that may exceed tolerable limits. the aim of this study was to determine the range of solution osmolalities that results in significant cha ... | 1999 | 10442740 |
| effects of preservation of rat lungs in a hypothermic medium on alveolar morphology. | the increasing use of organs such as liver, lung, heart, pancreas, kidney and small intestine for transplantation purposes necessitates the development of optimum preservation techniques. the aim of our study was to investigate time-related morphological changes in alveoli during preservation of rat lungs in hypothermic euro-collins solution. lungs were perfused via the pulmonary arteries with euro-collins solution at a temp of 19 degrees c. totally perfused lungs were placed in euro-collins sol ... | 1999 | 10443296 |
| inhaled nitric oxide as a prophylactic treatment against reperfusion injury of the lung. | inhaled nitric oxide (no) has been found to be effective in clinical treatment of reperfusion injury after lung transplantation. this study was designed to determine a possible prophylactic role to reduce severe reperfusion injury. | 1999 | 10443521 |
| analysis of 31 cftr mutations by polymerase chain reaction/oligonucleotide ligation assay in a pilot screening of 4476 newborns for cystic fibrosis. | molecular biological testing for genetic diseases has grown rapidly, but speed, accuracy, specificity, sensitivity, throughput, and cost become more important as large scale screening is considered. this is a pilot study of an assay for the simultaneous detection of up to 31 cystic fibrosis mutations in a multicentre population based screening of 4476 italian newborns. | 1999 | 10444722 |
| combined use of prostacyclin and higher perfusate temperatures further enhances the superior lung preservation by celsior solution in the isolated rat lung. | the poor tolerance of the lung to ischemia and reperfusion (ir) still represents one of the limitations in clinically successful lung transplantation. modified euro-collins (ec) is routinely used in lung preservation, but alternative solutions have been developed for improvement of pulmonary preservation. celsior is an extracellular solution that has significantly reduced the ir-induced pulmonary damage in animal studies. so far, no extensive experimental studies exist concerning the influence o ... | 1999 | 10452345 |
| [cost analysis concerning mrsa-infection in icu]. | mrsa-infection incidences are still rising, because of unreflected use of antibiotic drugs in man and animals. although some european countries already have an incidence as high as 30% of mrsa infection in icu-patients, there is no additional financial support for the treatment of mrsa infected patients. | 1999 | 10464519 |
| forskolin-stimulated adenylylcyclase activity: a marker to assess islet cell viability following cold storage in different solutions and to predict islet cell function following transplantation. | for clinical islet cell transplantation, short-term storage of islet cells is likely to be necessary, and it is imperative that the islet cells be kept as viable as possible during the period. however, there are little data on which preservative solutions are most suitable for the storage of islet cells after isolations or before transplantation. to estimate islet cell viability and transplantation success rate in the present study, adenylylcyclase activity was measured with a rapid new fluorome ... | 1999 | 10478718 |
| amelioration of lung reperfusion injury by l- and e- selectin blockade. | reperfusion injury is the main reason for early graft failure after lung transplantation. inhibition of the adherence of polymorphonuclear leukocytes to activated endothelium by blocking l- and e-selectins (antibody el-246) could potentially inhibit reperfusion injury. | 1999 | 10485417 |
| skin flap storage with intracellular and extracellular solutions containing trehalose. | to evaluate and compare the effects of it-kyoto (it-k) solution (intracellular type) and et-kyoto (et-k) solution (extracellular type) on long-term skin flap preservation (from 24-120 hours), japanese white rabbits were used as an animal model and euro-collins (ec) solution was used as a comparative solution. a total of 180 animals were divided randomly into 15 groups (n = 12) for 24, 48, 72, 96, and 120 hours of flap storage in three solutions. the survival rate, weight change, and histological ... | 1999 | 10490181 |
| organ preservation with ec, htk, and uw, solution in orthotopic rat liver transplantation. part ii. morphological study. | the quality of organ preservation is of major importance in minimizing the incidence of primary graft nonfunction and organ rejection. for this study a new semiquantitative score was developed that grades morphologic tissue alterations in the liver according to their frequency and severity. it was applied to assess commonly used perfusion solutions for their efficacy in preventing early and late tissue damage after rat liver transplantation. for transplantation the livers were stored in euro-col ... | 1999 | 10501078 |
| perfusion of kidneys with unformulated "naked" intercellular adhesion molecule-1 antisense oligodeoxynucleotides prevents ischemic/reperfusion injury. | we have previously shown that phosphorothioate intercellular adhesion molecule (icam)-1 antisense oligodeoxynucleotide (oligo) ip-9125 blocks the expression of rat icam-1 mrna in rat l2 cells. a single ex situ perfusion of grafts with unformulated ip-9125, suspended in euro-collins solution, prolonged the survival of kidney allografts in rats. the present experiments examined whether perfusion of kidneys with unformulated ip-9125 prevents ischemic/reperfusion injury. | 1999 | 10515390 |
| comparison of (32)p-postlabeling and high-resolution gc/ms in quantifying n7-(2-hydroxyethyl)guanine adducts. | this study compares (32)p-postlabeling and high-resolution gas chromatography/mass spectrometry (gc/ms) in the quantification of n7-(2-hydroxyethyl)guanine adducts (7-heg) in dna obtained from the same tissue samples of control rats and rats exposed to ethene. the samples were obtained from two independent studies. in one study, male sprague-dawley rats were exposed to 300 ppm ethene for 12 h/day for 3 days ("euro samples"). in the other study, male f-344 rats were exposed to 3000 ppm ethene for ... | 1999 | 10525275 |
| donor lung pretreatment with prostaglandin e(1) does not improve lung graft preservation. | prostaglandin e(1) (pge(1)) is widely used to improve early graft function after lung transplantation, but some studies have questioned its benefits. therefore we evaluated the effect of donor pretreatment with pge(1) in our porcine model of single lung transplantation. donors received pge(1) or placebo intravenously before flushing the pulmonary artery with modified euro-collins solution. after cold storage, the excised left lung was transplanted. ischemic time was 4 h. we used our right side h ... | 1999 | 10529557 |
| oocyte donor selection from 554 candidates. | oocyte donation is a technique in full expansion in the field of human reproduction. the main problem with this technique is the shortage of oocytes. in our programme, prospective donors are selected from anonymous, well-informed university students over 18 years of age, who give their informed consent in writing. before being accepted as donors, the candidates' personal and family medical histories were taken and they were given a gynaecological examination, genital ultrasonography, and analyse ... | 1999 | 10548620 |
| lung procurement by low-potassium dextran and the effect on preservation injury. munich lung transplant group. | this clinical study was performed to evaluate the effect of low-potassium dextran (lpd) solution on organ function in human lung transplantation. | 1999 | 10551643 |
| management of symptomatic bph in the uk: who is treated and how? | to review the contemporary management of symptomatic benign prostatic hyperplasia (bph) in the united kingdom (uk). | 1999 | 10559629 |
| selective adenosine-a2a activation reduces lung reperfusion injury following transplantation. | the adenosine-a2a receptor on the neutrophil is responsible for several anti-inflammatory actions. we hypothesized that dwh-146e, a selective adenosine-a2a agonist, would reduce lung reperfusion injury following transplantation. | 1999 | 10561110 |
| identification of optimal conditions for lung graft storage with euro-collins solution by use of a rat orthotopic lung transplant model. | lung preservation disrupts normal vascular homeostasis, resulting in increased permeability, vasoconstriction, and endothelial cell adhesion for neutrophils. we hypothesized that a storage strategy that best preserves post-lung transplantation (ltx) vascular homeostasis might be organ and species specific. because of the potential utility of a rat ltx model for developing improved lung preservation strategies, we have attempted to identify the optimal physical conditions for rat lung graft stora ... | 1999 | 10567313 |
| pharmacological limitation of damage to renal medulla after cold storage and transplantation by trimetazidine. | delayed graft function remains an important complication after renal transplantation. in this study, we investigated the influence of trimetazidine (tmz), a cytoprotective agent, on renal medullary damage after prolonged preservation and autotransplantation. pig kidneys were cold-flushed and preserved (48 h at 4 degrees c) with two standard renal preservation solutions euro-collins and university of wisconsin supplemented or not with tmz (10(-6) m). analysis of plasma and urine from 48-h-cold-st ... | 2000 | 10604955 |
| improvement of pulmonary preservation with celsior and perfadex: impact of storage time on early post-ischemic lung function. | optimal preservation of post-ischemic organ function is a continuing challenge in clinical lung transplantation. | 1999 | 10612378 |
| trimetazidine reduces renal dysfunction by limiting the cold ischemia/reperfusion injury in autotransplanted pig kidneys. | ischemia/reperfusion injury leads to delayed graft function, which is a major problem in kidney transplantation. this study investigated the effects of adding trimetazidine (tmz) to the perfusate of cold-stored kidneys on the function of reperfused autotransplanted pig kidney. the left kidney was removed and cold-flushed with euro-collins (ec), or university of wisconsin (uw) solutions with or without 10(-6)m tmz and stored for 48 h at 4 degrees c. the kidneys were then autotransplanted and the ... | 2000 | 10616850 |
| economic evaluation of influenza vaccination. assessment for the netherlands. | the objective of this study was to determine the costs associated with influenza and the cost effectiveness (net costs per life-year gained) of influenza vaccination in the netherlands. | 1999 | 10623374 |
| self-report measures and scoring protocols affect prevalence estimates of meeting physical activity guidelines. | prevalence estimates of meeting the guidelines for physical activity based on various self-report measures were compared, and the effects of various scoring protocols on the estimates were evaluated. | 2000 | 10647542 |
| [the costs of treating hiv-infected children in abidjan, ivory coast, 1996-1997]. | little is known about the costs of treating hiv-infected children in africa. however, this is one of the factors that must be taken into account when assessing the cost-effectiveness of strategies aimed at reducing the transmission of hiv from mother to child. the aim of this study was to estimate the direct costs of the treatment of african children born to hiv-infected mothers and the additional costs of treating those children who are themselves infected with the virus. we assessed the direct ... | 1999 | 10657769 |
| direct cost of single dose methotrexate for unruptured ectopic pregnancy. prospective comparison with laparoscopy. | to compare the direct cost of single dose methotrexate (mtx) and laparoscopy in the treatment of unruptured ectopic pregnancy (unruptured ep). | 2000 | 10659909 |
| nineteenth century canada: indigenous place of dis-ease. | during the nineteenth century period of intensive european expansion into canada, place was experienced with dis-ease by indigenous people. not only was there less land available for people of the first nations to live on as in the past centuries, but their intimate relationship with the land was disturbed causing a dis-ease, as their ability to experience place through ceremony was denied. the effects of this process of euro-canadian invasion within canada created a sense of dis-ease, a sense o ... | 1998 | 10671011 |
| raffinose improves the function of rat pulmonary grafts stored for twenty-four hours in low-potassium dextran solution. | the perfect strategy for pulmonary graft preservation remains elusive. experimental work supports the use of perfusates, such as euro-collins, university of wisconsin, and low-potassium dextran solutions. we use low-potassium dextran solution in our clinical program, but we aim for continued improvement. the trisaccharide raffinose has been shown to be responsible for the efficacy of university of wisconsin perfusate in lung preservation. raffinose is superior to a variety of other saccharides f ... | 2000 | 10694607 |
| improved quality and yield of islets isolated from human pancreata using a two-step digestion method. | a new approach, involving a two-step digestion process and los angeles preservation solution #1 (lap-1), a cold storage solution, was developed for isolation of high-quality islets from human pancreata for transplantation. this approach markedly improves the islet yield, purity and viability, and the isolation success rate. in this method, the pancreas was digested first in warm collagenase solution for up to 20 minutes. after decanting the enzyme solution, partially digested tissue was dissocia ... | 2000 | 10707935 |
| effects of cold and warm ischemia on the mitochondrial oxidative phosphorylation of swine lung. | the aim of the study was to investigate the consequence of warm and cold ischemia on lung mitochondria in order to define bioenergetic limits within lung could be suitable for pulmonary transplantation. | 2000 | 10708115 |
| the effect of fk409-a nitric oxide donor-on canine lung transplantation. | nitric oxide (no) is known to have beneficial effects in ischemia-reperfusion (i/r) injury through maintaining endothelial integrity, inhibiting leukocyte adhesion and platelet aggregation, and inducing vasodilation. the effect of fk409 (fk), a spontaneous no donor, was investigated in a canine lung transplantation model. | 2000 | 10713255 |
| limitation of ischemic damage to the renal medulla by trimetazidine added to euro-collins solution: evaluation in an autotransplant model. | | 2000 | 10715485 |
| effect of tt virus infection on hepatocellular carcinoma development: results of a euro-asian survey. | a small percentage of persons with hepatocellular carcinoma (hcc) lack identifiable causes of liver pathology. the single-stranded dna virus, tt virus (ttv), has been found in persons with acute and chronic liver injury. nested polymerase chain reaction was used to search for both ttv and parvoviruses in 293 hcc samples from asia and europe. ttv was found in >30% of chinese and italian samples but in only 13% of french samples. no clinicopathologic differences were found between ttv-positive and ... | 2000 | 10720542 |
| value of pedigree/clinical data, immunohistochemistry and microsatellite instability analyses in reducing the cost of determining hmlh1 and hmsh2 gene mutations in patients with colorectal cancer. | the aim of this study was to evaluate the significance of pedigree/clinical data, immunohistochemistry (ihc) and microsatellite instability (mi) analyses in the reduction of costs of constitutional hmlh1 and hmsh2 gene mutation diagnosis in patients with colorectal cancers (crc). pedigree/clinical data were evaluated on a series of 168 patients with crc, including 43 consecutive sporadic late-onset and 25 consecutive, definitive or suspected hereditary non-polyposis colorectal cancer (hnpcc) cas ... | 2000 | 10741294 |
| analysis of the 5.8s rrna gene and the internal transcribed spacers in naegleria spp. and in n. fowleri. | internal transcribed spacers (its) and the 5.8s ribosomal gene of 21 naegleria fowleri strains and eight other species including naegleria gruberi were sequenced. the results showed that this region can help differentiate between and within species. the phylogeny of naegleria spp. deduced from the its and the 5.8s gene produced four major lineages, fowleri-lovaniensis, galeacystis-italica-clarki-gruberi-australiensis, andersoni-jamiesoni, and pussardi, that fit perfectly with those inferred from ... | 2000 | 10750838 |
| the effect of the two-layer cold storage method on islet isolation from ischemically damaged pancreas. | previous studies have shown that the two-layer cold storage method (tl) resuscitates the function of warm ischemically (wi) damaged pancreas when transplanted as a segmental graft in a dog model. in this study, we evaluated the effect of tl on yield and viability of islets isolated from wi damaged pancreas. in group 1 (n = 10), dog pancreases were processed into islets immediately after total pancreatectomy. in group 2 (n = 5), excised pancreases were left in the abdominal cavity for 60 minutes ... | 1999 | 10752312 |
| preservation of intraalveolar surfactant in a rat lung ischaemia/reperfusion injury model. | ischaemia/reperfusion (i/r) injury, a major problem in clinical lung transplantation, is associated with surfactant dysfunction. the present study aimed to test the hypothesis that preservation related improvements in post-ischaemic lung function are associated with improved ultrastructural preservation of pulmonary surfactant. rat lungs were flush perfused with modified euro-collins solutions (ecs), stored for 2 h at 4 degrees c, and reperfused for 40 min. lungs were preserved with conventional ... | 2000 | 10759447 |
| diagnosis of helicobacter pylori after triple therapy in uncomplicated duodenal ulcers--a cost-effectiveness analysis. | the cost-effectiveness of determining helicobacter pylori status after treatment remains to be established. | 2000 | 10759623 |
| the effects of various organ preservation solutions on hepatocyte membrane potentials, intracellular calcium concentrations, and outcome following liver transplantation. | hepatocyte membrane potential differences (pds) may be altered by the preservation solutions used in liver transplantation. such alterations could impact on the survival of the donor liver, extent of biochemical injury, and flux of important ionic compounds. the purpose of the present study was to document these outcomes in the presence of four different preservation solutions. | 2000 | 10773153 |
| hmg20a and hmg20b map to human chromosomes 15q24 and 19p13.3 and constitute a distinct class of hmg-box genes with ubiquitous expression. | the hmg box encodes a conserved dna binding domain found in many proteins and is involved in the regulation of transcription and chromatin conformation. we describe hmg20a and hmg20b, two novel human hmg box-containing genes, discovered within the euro-image consortium full-length cdna sequencing initiative. the predicted proteins encoded by these two genes are 48.4% identical (73.9% within the hmg domain). the hmg domain of both hmg20 proteins is most similar to that of yeast nhp6a (38% to 42%) ... | 2000 | 10773667 |
| comparison of university of wisconsin, euro-collins, low-potassium dextran, and krebs-henseleit solutions for hypothermic lung preservation. | we sought to test the effectiveness of 4 different solutions for hypothermic rat lung preservation. | 2000 | 10788813 |
| improved biochemical preservation of lung slices during cold storage. | development of lung preservation solutions typically requires whole-organ models which are animal and labor intensive. these models rely on physiologic rather than biochemical endpoints, making accurate comparison of the relative efficacy of individual solution components difficult. we hypothesized that lung slices could be used to assess preservation of biochemical function during cold storage. | 2000 | 10792955 |
| beneficial effect of additional cardioplegia flush during hypothermic static cardiac preservation. | the effects of various preservative solutions and methods have been studied to prolong the safety period of cardiac preservation. in this study, we used cardioplegic solution (cs) during cardiac preservation and investigated how flush cs yields good preservation of isolated hearts compared with only cold immersion. | 2000 | 10830237 |
| homozygosity mapping in a family with microcephaly, mental retardation, and short stature to a cohen syndrome region on 8q21.3-8q22.1: redefining a clinical entity. | a syndrome of microcephaly, progressive postnatal growth deficiency, and mental retardation was observed in two brothers and their cousin from a multiply consanguineous kindred of lebanese descent. hypotonia, chorioretinal dystrophy, and myopia were also identified. the severity of the condition varied among the closely related patients. because of absence of a distinctive facial appearance, the degree of mental retardation, and short stature, the initially considered clinical diagnosis of cohen ... | 2000 | 10842298 |
| the effects of inhaled nitric oxide, gabexate mesilate, and retrograde flush in the lung graft from non-heart beating minipig donors. | the use of lung grafts from non-heart-beating donors (nhbd) is one way of solving the donor organ shortage problem. in this experiment, we studied the effect of retrograde flush (rf) from the left atrium before harvest, inhaled nitric oxide (no), and gabexate mesilate (foy), a protease inhibitor, in the lung grafts from nhbd. | 2000 | 10852590 |
| antimicrobial properties of copper-coated electroconductive polyester fibers. | three synthetic copper-coated euro-static fibers (pet--polyester, pa--polyamide, and pac--polyacrylamide) manufactured by europa corporation s.c., poland, were tested as potential antimicrobial agents. the inhibitory properties of the fibers were examined using different microorganisms as follows: i. staphylococcus aureus atcc 25293, and pseudomonas aeruginosa atcc 27853 reference strains, ii. 8 strains of s. aureus (4 mrsa and 4 mssa) and 5 strains of p. aeruginosa isolated from infected wounds ... | 1999 | 10876646 |
| iodine supply in children from different european areas: the euro-growth study. committee for the study of iodine supply in european children. | in many areas of europe iodine deficiency disorders still exist. urinary iodine excretion is a valuable tool in characterizing the iodine supply of a population. however, there is a continuing discussion on the most appropriate parameter to measure urinary iodine excretion. | 2000 | 10896091 |
| [the malaria situation in the who european region]. | the number of indigenous malaria cases in european region peaked in 1997, when 77,985 cases were officially reported. these were caused almost exclusively by p. vivax, p. falciparum being restricted to a rather limited number of cases in tajikistan only. another important problem in the european region is the importation of malaria associated with a high fatality rate from tropical endemic countries. there were 841 cases of malaria in armenia, 567 of which were locally transmitted, 30 out of 81 ... | 2000 | 10900907 |
| levels and trends of pcdd/fs and pcbs in human milk in finland. | word health organization, who/euro, has coordinated two rounds of follow-up studies on levels of pcdds, pcdfs, and pcbs in human milk which were analyzed as two pooled samples from each participating country, one from urban and the other one from rural area. finland has taken part to both of those studies and we are now reporting results of all the second round randomly sampled human milk samples (84 samples) from southern (20) and eastern (64) finland. the levels of pcdd/fs and pcbs in human mi ... | 1999 | 10901657 |
| influence of cold-storage conditions on renal function of autotransplanted large pig kidneys. | the consequences of ischemia/reperfusion injury (iri) on delayed graft function (dgf) and graft survival for kidney recipients remain a matter of debate. several strategies have been proposed to reduce iri. we have shown that adding the anti-ischemic drug trimetazidine (tmz) to different preservation solutions had beneficial effects on the function of reperfused rat and pig kidneys. | 2000 | 10916109 |
| ultrastructural and functional studies of cryopreserved rat lungs for transplantation using a new hyperosmolal solution. | one of the most promising approaches for extending the period of tissue or organ preservation is to induce a state of cryopreservation. in this report, to achieve successful lung cryopreservation, we created a new hyperosmolal solution (hos) containing 10% glycerol and 5% dimethyl sulfoxide (me2so). we compared the efficacy of hos with that of euro-collins solution (ecs) in rat lung cryopreservation from an ultrastructural and functional point of view. using ecs, widespread damage to the air-blo ... | 2000 | 10920605 |
| effects of storage and reperfusion oxygen content on substrate metabolism in the isolated rat lung. | lung transplantation requires a period of storage and ischemia; we examined the largely unknown effects of that period on intermediary metabolism. | 2000 | 10921720 |
| effect of hypothermic pulmonary artery flushing on capillary filtration coefficient. | we previously demonstrated that surfactant dilution and inhibition occur immediately after pulmonary artery flushing with hypothermic modified euro-collins solution. consequently, we speculated that increased capillary permeability contributed to these surfactant changes. to test this hypothesis, we evaluated the effects of hypothermic pulmonary artery flushing on the pulmonary capillary filtration coefficient (kfc), and additionally performed a biochemical analysis of surfactant. | 2000 | 10933147 |
| influence of retrieval conditions on renal medulla injury: evaluation by proton nmr spectroscopy in an isolated perfused pig kidney model. | delayed graft function (dgf) has remained an important complication after renal transplantation. the exact causes of dgf remain to be clarified, particularly the impact of retrieval conditions and preservation factors. in the present investigation, (1)hnmr spectroscopy of urine was assessed in order to detect the influence of retrieval condition on renal medulla damage. | 2000 | 10945936 |
| a rapid, simple, and cost-effective method for screening liver preservation solutions in the rat. | rat liver transplantation models or isolated liver perfusion models are currently used for assessing efficacy of liver preservation methods. we tested the hypothesis that hepatocellular enzymes released into the washout solution after preservation may predict hepatic function during reperfusion and could thus be alternatively used for evaluating efficiency of liver preservation solutions. furthermore, we applied this approach for assessing the role of kupffer cells (kc) in preservation-induced l ... | 2000 | 10949183 |
| [cost-effectiveness analysis of 2 strategies of helicobacter pylori eradication: results of a prospective and randomized study in primary care]. | to analyze cost-effectiveness of two different strategies to treat h. pylori infection in peptic ulcer in the primary care setting. | 2000 | 10953829 |
| attenuation of lung reperfusion injury after transplantation using an inhibitor of nuclear factor-kappab. | a central role for nuclear factor-kappab (nf-kappab) in the induction of lung inflammatory injury is emerging. we hypothesized that nf-kappab is a critical early regulator of the inflammatory response in lung ischemia-reperfusion injury, and inhibition of nf-kappab activation reduces this injury and improves pulmonary graft function. with use of a porcine transplantation model, left lungs were harvested and stored in cold euro-collins preservation solution for 6 h before transplantation. activat ... | 2000 | 10956628 |
| low-potassium dextran solution ameliorates reperfusion injury of the lung and protects surfactant function. | this study was designed to compare the effect of lung preservation with low-potassium dextran solution and euro-collins solution on reperfusion injury and surfactant function by using an in situ model of warm ischemia. | 2000 | 10962420 |
| the effect of nitecapone on early graft function in experimental single lung transplantation. | nitecapone is an antioxidant molecule which has been shown to protect the heart against ischemia-reperfusion injury. we investigated whether a similar effect could be detected on lung graft preservation in a porcine model of single lung transplantation. donors received either nitecapone or placebo in a modified euro-collins pulmonary flush solution. after cold storage for 19 h the left lung was transplanted. patients in the nitecapone group received a nitecapone infusion during the graft reperfu ... | 2000 | 10983677 |
| hiv risks in a homeless population. | homeless people are one of the most vulnerable with regard to hiv transmission. however, most research on this population has been carried out on samples from health clinics. we surveyed 390 homeless people in houston at a day shelter with regard to their hiv/aids knowledge and risk behaviours. the sample was 76% african-american, 11% euro-american, with small numbers of latin-americans, native-americans and asian-americans: half were born in texas, and 92% were male. data indicated that hiv/aid ... | 2000 | 10990335 |
| clinical andrology: from evidence-base to ethics. the 'e' quintet in clinical andrology. | the management of the infertile man should be founded on consensus-based medicine, i.e. the consensual opinion of experts considering evidence-based as well as empirical or experience-based medicine, the effective cumulative rate of successful deliveries, ethical and economic considerations. the apparent contradictions between conclusions from experience-based medicine and evidence-based medicine regarding the efficacy of varicocele treatment and tamoxifen treatment can be explained by scientifi ... | 2000 | 11006174 |
| fr167653 ameliorates pulmonary damage in ischemia-reperfusion injury in a canine lung transplantation model. | interleukin (il)-1 and tumor necrosis factor-alpha (tnf-alpha) are recognized as important factors in ischemia-reperfusion (i/r) injury. fr167653 has been characterized as a potent suppressant of il-1 and tnf-alpha production. we previously reported that fr167653 suppressed the expression of il-1 beta mrna after reperfusion and ameliorated pulmonary i/r injury following 3-hour left lung warm ischemia in dogs. the aim of this study was to investigate the effects of fr167653 on i/r injury in a can ... | 2000 | 11008078 |
| effects of ep4 solution and lpd solution vs euro-collins solution on na(+)/k(+)-atpase activity in rat alveolar type ii cells and human alveolar epithelial cell line a549 cells. | intact alveolar epithelial na(+)/k(+)- adenosinetriphosphatase (atpase) function is important in preventing alveolar fluid accumulation after lung transplantation. we examined whether the type of preservation solution used influences na(+)/k(+)-atpase activity in alveolar epithelial cells. | 2000 | 11008079 |
| the economic impact of smoking in germany. | smoking is a high-risk behaviour affecting health and economic welfare of society. thus it is important to quantify the economic burden smoking places on social institutions in germany. approximately 33.4% of the male and 20.4% of the female population are current smokers. this study investigates the health care costs of smoking based on 1996 figures, focusing on the seven most frequent diseases associated with the inhalation of tobacco smoke: chronic obstructive pulmonary disease (copd, interna ... | 2000 | 11028648 |
| biochemical changes in transplanted rat liver stored in university of wisconsin and euro-collins solutions. | liver ischemia/reperfusion injury is a severe problem in transplantation, and preservation solutions could be critical for liver viability. the aim of our study was to evaluate the cytosolic and mitochondrial glutathione levels, the glyoxalase ii activity, and the mitochondrial hydroperoxide contents of livers stored in different preservation solutions for 7 or 24 h and after transplantation. | 2000 | 11038305 |
| retrograde flush perfusion with low-potassium solutions for improvement of experimental pulmonary preservation. | optimal preservation of post-ischemic organ function is a continuing challenge in clinical lung transplantation. retrograde instillation of preservation solutions has the theoretic advantage of achieving homogeneous distribution in the lung because of perfusing both the pulmonary and the bronchial circulation. so far, we have seen no experimental studies that include stereologic analysis of intrapulmonary edema concerning the influence of retrograde preservation on post-ischemic lung function af ... | 2000 | 11044693 |
| matrix metalloproteinases correlate with alveolar-capillary permeability alteration in lung ischemia-reperfusion injury. | matrix metalloproteinases (mmps) are able to degrade the endothelial basal lamina and increase vascular permeability. | 2000 | 11045633 |
| modulation of pulmonary na+ pump gene expression during cold storage and reperfusion. | reperfusion injury with pulmonary edema continues to be a major complication after lung transplantation. alveolar fluid homeostasis is regulated by na+/k+-atpase activity on the basolateral surface of alveolar epithelial cells. intact na+/k+-atpase is essential to the resolution of pulmonary edema. we characterized the effects of cold ischemia and reperfusion on expression of na+/k+-atpase mrna and protein. | 2000 | 11045636 |
| aprotinin improves pulmonary function during reperfusion in an isolated lung model. | we hypothesized that the use of aprotinin would ameliorate the reperfusion injury observed after lung transplantation because of a reduction in the inflammatory response. | 2000 | 11093508 |
| ascorbic acid for amelioration of reperfusion injury in a lung autotransplantation model in sheep. | reperfusion injury is the leading cause of early graft dysfunction after lung transplantation. activation of neutrophilic granulocytes with generation of free oxygen radicals appears to play a key role in this process. the efficacy of ascorbic acid as an antioxidant in the amelioration of reperfusion injury after lung transplantation has not been studied yet. | 2000 | 11093511 |
| resistance of isolated pulmonary mitochondria during in vitro anoxia/reoxygenation. | the aim of the study was to investigate the effect of in vitro anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. mitochondria were isolated after harvesting from fresh pig lungs flushed with euro-collins solution. mitochondrial respiratory parameters were determined in isolated mitochondria before anoxia (control), after 5-45 min anoxia followed by 5 min reoxygenation, and after 25 or 40 min of in vitro incubation in order to follow the in vitro aging of mitoch ... | 2000 | 11106775 |
| effect on rat kidney preservation in the cold of various sugars added to euro-collins solution to adjust osmolarity. | | 2000 | 11119865 |
| comparison of euro-collins and university of wisconsin solutions in the isolated perfused rat liver model: evaluation by proton nuclear magnetic resonance spectroscopy. | | 2000 | 11134808 |
| influence of an infectious disease service on antibiotic prescription behavior and selection of multiresistant pathogens. | a routine infectious disease service was established in january 1998 in order to optimize the antibiotic usage and prescription pattern of a neurologic intensive care unit (nicu). | 2000 | 11139159 |
| disease management status: a typology of latino and euro-american patients with type 2 diabetes. | the number of indicators of chronic disease management is large and no practical framework is available to guide selection for use in intervention programs. in addition, it is often difficult to integrate multiple disease management indicators into a comprehensive picture of patient functioning. the authors present a heuristic framework for creating a profile of disease management and an empirically based descriptive typology of disease management behavior. from the literature, they identified 5 ... | 2000 | 11147290 |
| population variation in children's behavioral symptomatology. | previous anthropological studies identified significant interpopulation consistencies in the frequency and symptoms of adult depression, anxiety, and schizophrenia. tests of the degree of variation of such behavioral phenomena across contemporary populations are significant for the study of human evolution because universality or near universality suggests specific evolved aspects of human behavior. the aim of this research was to provide a preliminary test of whether cross-cultural consistencie ... | 2001 | 11150052 |
| protection of autotransplanted pig kidneys from ischemia-reperfusion injury by polyethylene glycol. | ischemia-reperfusion injury (iri) is often responsible for graft rejection and leads to delayed graft function of cadaveric kidneys. we have shown that adding polyethylene glycol (peg 20m) to the preservation solutions helps protect isolated perfused pig kidneys against cold ischemia and reperfusion injury. | 2000 | 11152217 |
| kidney retrieval conditions influence damage to renal medulla: evaluation by proton nuclear magnetic resonance (nmr) pectroscopy. | in the present investigation, the influence of retrieval condition on medullary damage in kidneys was assessed. the isolated perfused pig kidney was used to assess initial renal function from multiorgan donors or single organ donors after cold flush and 24 h cold storage preservation with two preservation solutions: euro-collins and university of wisconsin solutions. kidneys flushed with cold heparinized saline and immediately perfused were used as a control group. kidneys were perfused for 90 m ... | 2000 | 11156333 |
| citrate, acetate and renal medullary osmolyte excretion in urine as predictor of renal changes after cold ischaemia and transplantation. | in organ transplantation, the determination of reliable parameters to assess ischaemic damage is essential to predict renal injury after preservation. the aim of this study was to assess renal medullary injury by 1h nmr (proton nuclear magnetic resonance) spectroscopy after preservation and reperfusion. three experimental groups of pigs were examined during a 2-week period: control group (n = 4), euro-collins group (ec) (cold flushed and 48 h cold storage of kidney in ec and autotransplantation, ... | 2000 | 11156334 |
| a new approach to the evaluation of liver graft function by nuclear magnetic resonance spectroscopy. a comparative study between euro-collins and university of wisconsin solutions. | the incidence of primary dysfunction or non-function of liver grafts still occurs at an unacceptable rate and the identification of new markers of graft viability and metabolic capacity is essential. proton nuclear magnetic resonance (h nmr) spectroscopy has previously shown potential in the evaluation of renal allograft dysfunction after ischaemia reperfusion. the aim of this study was to compare liver graft function in a rat isolated perfused liver model after 24 hours of preservation in eithe ... | 2000 | 11156343 |
| risk factors for aids associated kaposi's sarcoma. an european multicentre case control study. | euro-shaks, european study on hiv associated kaposi's sarcoma, is a biomed 1 project financed by the european union (dg xii). the spanish side of the project has been financed by fiss (fondo de investigaciones sanitarias). the aims of this study are to identify possible genetic, behavioural, biological and environmental risk factors for hiv associated kaposi's sarcoma through a multicentre case-control study. an extensive personal questionnaire, a clinical data form and blood sample is required ... | 1997 | 11173630 |
| antiretroviral treatments used among adults with hiv infection in europe. | international guidelines for the treatment of human immunodeficiency virus (hiv) infection change rapidly. they are mainly based on results from large-scale randomized clinical trials, but also on hypotheses. the objective of the study was to look at the use of antiretrovirals (arvs) in different hiv treatment centres in europe. between august 1996 and september 1997 self-administered anonymous questionnaires were distributed to persons with hiv infection at inpatient and outpatient departments ... | 2001 | 11177461 |
| ischemic preconditioning enhances donor lung preservation in canine lung transplantation. | to prove the protective effect of lung ischemic preconditioning on enhancing the canine lung preservation and reducing allograft lung dysfunction after transplantation. | 1998 | 11189228 |
| ethnic differences in patterns and correlates of age of initiation in a population of air force recruits. | early age of initiation is a significant risk factor for long-term dependent smoking and may also relate to other unhealthy behaviors. the current study assessed age of initiation in relationship to smoking dependence and motivation to quit, physical activity, dietary intake, body mass index (bmi), attitude toward illegal drug use, binge drinking, seat belt use, and smoking status at follow-up. subjects were 7995 air force recruits who reported smoking regularly up to basic military training. eu ... | 2000 | 11197313 |
| noninvasive monitoring of citrate, acetate, lactate, and renal medullary osmolyte excretion in urine as biomarkers of exposure to ischemic reperfusion injury. | injury during the transplant process affects the alloantigen-dependent factors and the alloantigen-independent processes of "chronic" rejection. consequently, the determination of reliable parameters for the assessment of ischemic damage is essential for the prediction of renal changes after ischemia/reperfusion injury. the aim of this study was to assess the ability of (1)h nmr spectroscopy to predict the early graft dysfunction in an ischemia/reperfusion model after preservation in two standar ... | 2000 | 11222025 |
| economic impact of community- and nosocomially acquired rotavirus gastroenteritis in austria. | to determine the average costs per child for rotavirus (rv) acute gastroenteritis from different perspectives, from the hospital's, third-party payer's, pediatrician's and family's perspectives as well as in summary from the societal one. | 2001 | 11224839 |