| influence of rpos, camp-receptor protein, and ppgpp on expression of the opggh operon and osmoregulated periplasmic glucan content of salmonella enterica serovar typhimurium. | a transcriptional fusion (opgg1::mudj) to the opggh operon of salmonella enterica serovar typhimurium (s. typhimurium) lt2, isolated by resistance to mecillinam, was used to study the influence of global regulators rpos, ppgpp, and camp/camp-receptor protein (crp) on expression of the opggh operon and osmoregulated periplasmic glucan (opg) content. neither high growth medium osmolarity nor absence of ppgpp or crp had important effects on opgg1::mudj expression in exponential cultures. however, u ... | 2009 | 19940937 |
| quantitative analysis of the immune response upon salmonella typhimurium infection along the porcine intestinal gut. | salmonella enterica serovar typhimurium causes enteric disease and compromises food safety. in pigs, the molecular response of the intestine to s. typhimurium has been traditionally characterized by in vitro models that do not reflect the actual immunological competence of the intestinal mucosa. in this work, we performed an oral s. typhimurium infection study to obtain insight into the in vitro response in three different sections (jejunum, ileum and colon) of the porcine intestine. for this, s ... | 2010 | 19941811 |
| immobilization of bacteriophages on modified silica particles. | bacteriophages are selective anti-bacterial agents, which are receiving increasing acceptance by regulatory agencies for use both in the food industry and in clinical settings for biocontrol. while immobilized phage could be particularly useful to create antimicrobial surfaces, current immobilization strategies require chemical bioconjugation to surfaces or more difficult processes involving modification of their head proteins to express specific binding moieties, for example, biotin or cellulos ... | 2010 | 19945158 |
| some putative prebiotics increase the severity of salmonella enterica serovar typhimurium infection in mice. | prebiotics are non-digestible food ingredients believed to beneficially affect host health by selectively stimulating the growth of the beneficial bacteria residing in the gut. such beneficial bacteria have been reported to protect against pathogenic infections. however, contradicting results on prevention of salmonella infections with prebiotics have been published. the aim of the present study was to examine whether s. typhimurium sl1344 infection in mice could be prevented by administration o ... | 2009 | 19948011 |
| in salmonella enterica, 2-methylcitrate blocks gluconeogenesis. | strains of salmonella enterica serovar typhimurium lt2 lacking a functional 2-methylcitric acid cycle (2-mcc) display increased sensitivity to propionate. previous work from our group indicated that this sensitivity to propionate is in part due to the production of 2-methylcitrate (2-mc) by the krebs cycle enzyme citrate synthase (glta). here we report in vivo and in vitro data which show that a target of the 2-mc isomer produced by glta (2-mc(glta)) is fructose-1,6-bisphosphatase (fbpase), a ke ... | 2010 | 19948794 |
| identification of the salmonella enterica damx gene product, an inner membrane protein involved in bile resistance. | the damx gene product of salmonella enterica serovar typhimurium is a protein located in the inner membrane. damx migrates as a 70-kda protein in sds-page even though the predicted protein size is 46 kda. synthesis of damx protein occurs in both exponential- and stationary-phase cultures. disruption of damx causes severe sensitivity to bile. lack of the outer membrane protein asma suppresses bile sensitivity in salmonella damx mutants. | 2010 | 19948803 |
| synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth. | we have previously described a murein hydrolase activity for the surface layer (s-layer) of lactobacillus acidophilus atcc 4356. here we show that, in combination with nisin, this s-layer acts synergistically to inhibit the growth of pathogenic gram-negative salmonella enterica and potential pathogenic gram-positive bacteria, staphylococcus aureus and bacillus cereus. in addition, bacteriolytic effects were observed for the gram-positive species tested. we postulate that the s-layer enhances the ... | 2010 | 19948852 |
| blacmy-2-positive inca/c plasmids from escherichia coli and salmonella enterica are a distinct component of a larger lineage of plasmids. | large multidrug resistance plasmids of the a/c incompatibility complex (inca/c) have been found in a diverse group of gram-negative commensal and pathogenic bacteria. we present three completed sequences from inca/c plasmids that originated from escherichia coli (cattle) and salmonella enterica serovar newport (human) and that carry the cephamycinase gene blacmy-2. these large plasmids (148 to 166 kbp) share extensive sequence identity and synteny. the most divergent plasmid, peh4h, has lost sev ... | 2010 | 19949054 |
| proteome of salmonella enterica serotype typhimurium grown in a low mg/ph medium. | to determine the impact of a low mg(2+)/ph defined growth medium (mgm) on the proteome of salmonella enterica serotype typhimurium, we cultured s. typhimurium cells in the medium under two different conditions termed mgm shock and mgm dilution and then comparatively analyzed the bacterial cells harvested from these conditions by a global proteomic approach. proteomic results showed that mgm shock and mgm dilution differentially affected the s. typhimurium proteome. mgm shock induced a group of p ... | 2009 | 19953200 |
| the salmonella spi2 effector ssei mediates long-term systemic infection by modulating host cell migration. | host-adapted strains of salmonella enterica cause systemic infections and have the ability to persist systemically for long periods of time despite the presence of a robust immune response. chronically infected hosts are asymptomatic and transmit disease to naïve hosts via fecal shedding of bacteria, thereby serving as a critical reservoir for disease. we show that the bacterial effector protein ssei (also called srfh), which is translocated into host cells by the salmonella pathogenicity island ... | 2009 | 19956712 |
| antimicrobial drug use and antimicrobial resistance in enteric bacteria among cattle from alberta feedlots. | the purpose of this study was to determine whether antimicrobial resistance (amr) in foodborne pathogens (escherichia coli o157, salmonella, and campylobacter) and non-type-specific e. coli obtained from fecal samples of feedlot cattle was associated with antimicrobial drug (amd) use. a secondary objective was to determine if amr in non-type-specific e. coli could be used as a predictor of amr in foodborne pathogens. fecal samples were collected from pen floors in 21 alberta feedlots during marc ... | 2010 | 19958100 |
| phosphoribosylpyrophosphate synthetase (prsa) variants alter cellular pools of ribose 5-phosphate and influence thiamine synthesis in salmonella enterica. | phosphoribosylamine (pra) is the first intermediate in the common purine/thiamine biosynthetic pathway and is primarily synthesized by the product of the purf gene, glutamine phosphoribosylpyrophosphate (prpp) amidotransferase (e.c. 2.4.2.14). past genetic and biochemical studies have shown that multiple mechanisms for the synthesis of pra independent of purf are present in salmonella enterica. here, we describe mutant alleles of the essential prsa gene, which encodes prpp synthetase (e.c. 2.7.6 ... | 2010 | 19959576 |
| size-dependent pcr inhibitory effect induced by gold nanoparticles. | in this work, the effect of gold nanoparticles (aunps) with diameters of around 5, 10 and 20 nm on pcr efficiency is evaluated respectively using a real-time pcr machine. gold nanoparticles show no obvious effect on pcr at low particle concentration. when the concentration is increased, pcr inhibition is observed. at the same particle concentration, gold nanoparticles of different sizes show different inhibitory effects on pcr. it is found that taq polymerase can interact with aunps. the interac ... | 2009 | 19964596 |
| design, expression and characterization of recombinant hybrid peptide attacin-thanatin in escherichia coli. | antimicrobial peptides will be attractive and potential candidates as peptide drugs because of their efficient action against microbes and low toxicity to mammal cells. to improve their antibacterial activity, some modifications needs to be made. in this research, the hybrid peptide gene attacin-thanatin with 642 bp in length with preferred codons of e. coli was generated using the technology of gene splicing by overlap extension. the gene was inserted in-frame into e. coli expression plasmid pe ... | 2010 | 19967452 |
| high prevalence of salmonella in tegu lizards (tupinambis merianae), and susceptibility of the serotypes to antibiotics. | species of tegu (tupinambis) are the largest lizards in south america. large numbers of these lizards are hunted; there is a vigorous trade in their skins and the meat is consumed by rural and native peoples. the animals are also bred in captivity, an economic activity for rural populations which can help in the animals' conservation. faecal samples from 30 captive-born tegus were analysed for the presence of salmonella in two separate samplings. in the first analysis, samples from 26 animals (8 ... | 2010 | 19968856 |
| evolutionary diversification of an ancient gene family (rhs) through c-terminal displacement. | rhs genes are prominent features of bacterial genomes that have previously been implicated in genomic rearrangements in e. coli. by comparing rhs repertoires across the enterobacteriaceae, this study provides a robust explanation of rhs diversification and evolution, and a mechanistic model of how rhs diversity is gained and lost. | 2009 | 19968874 |
| septic arthritis of the sternoclavicular joint caused by salmonella in a healthy person. | the sternoclavicular (sc) joint is an unusual site for septic arthritis in healthy people. it is mostly described as affecting intravenous drug abusers. we report a rare case of sternoclavicular osteo-arthritis caused by non-typhoid salmonella in an immunocompetent person. the patient presented with general weakness, dizziness, epigastric and anterior chest pain that had persisted for one week. peptic ulcer disease was diagnosed by gastro-intestinal endoscopy. computed tomography (ct) revealed f ... | 2009 | 19994814 |
| validation of double digest selective label database for sequenced prokaryotic genomes. | summary: a database for simulation of double digest selective label (ddsl) typing technique has been created and validated against a sequenced strain (salmonella enterica serovar typhimurium strain lt2). in silico bands were in agreement with experimental, and the technique was able to discriminate among strains belonging to the same species. when compared with other strain discrimination techniques, ddsl showed a higher discriminatory power. the database contains precomputed data which may be s ... | 2010 | 19996164 |
| molecular and pathogenic characterization of salmonella enterica serovar bovismorbificans strains of animal, environmental, food, and human origin in hungary. | in this study, we characterized 110 strains of salmonella enterica serovar bovismorbificans contaminating environment, animals, food of animal origin, and human, to assess their significance along the food chain in hungary. additionally, five strains from germany were tested for comparative purposes. characterization involved antibiotic susceptibility testing, class 1 integron detection by polymerase chain reaction, plasmid profiling, virulotyping (using virulence gene-specific polymerase chain ... | 2010 | 20001326 |
| salicylidene acylhydrazide-mediated inhibition of type iii secretion system-1 in salmonella enterica serovar typhimurium is associated with iron restriction and can be reversed by free iron. | salmonella enterica serovar typhimurium is an animal and zoonotic pathogen of worldwide importance. intestinal colonization, induction of enteritis and systemic translocation by this bacterium requires type iii protein secretion. strategies that target this process have the potential to control infection, pathology and transmission. we defined the global transcriptional response of s. typhimurium to inp0403, a member of a family of salicylidene acylhydrazides that inhibit type iii secretion (t3s ... | 2010 | 20002188 |
| the transcriptional programme of salmonella enterica serovar typhimurium reveals a key role for tryptophan metabolism in biofilms. | biofilm formation enhances the capacity of pathogenic salmonella bacteria to survive stresses that are commonly encountered within food processing and during host infection. the persistence of salmonella within the food chain has become a major health concern, as biofilms can serve as a reservoir for the contamination of food products. while the molecular mechanisms required for the survival of bacteria on surfaces are not fully understood, transcriptional studies of other bacteria have demonstr ... | 2009 | 20003355 |
| spray washing of tomatoes with chlorine dioxide to minimize salmonella on inoculated fruit surfaces and cross-contamination from revolving brushes. | chlorine dioxide (clo(2)) is an antimicrobial agent available for commercial produce washing. this study examined the efficacy of clo(2) at 5 parts per million (ppm) during spray washing of tomatoes (5.0 ml/s per fruit) for preventing salmonella enterica transfer from inoculated roller brushes to fruit and wash runoff. furthermore, the sanitizing effects of clo(2) during spray washing at low and high flow rates (5.0 and 9.3 ml/s per fruit, respectively) on tomato surfaces (nonstem scar areas) wi ... | 2009 | 20003724 |
| thioridazine protects the mouse from a virulent infection by salmonella enterica serovar typhimurium 74. | when administered to mice at doses of 100microg/mouse and 200microg/mouse, thioridazine (tdz) significantly protected animals from the lethality produced by a virulent strain of salmonella enterica serovar typhimurium and reduced the number of bacteria retrieved from the spleen, liver and heart blood. the protection conferred by tdz against a virulent salmonella infection is hypothesised to be due to a reduction in the 55kda virulence protein of the outer membrane of the organism, as this protei ... | 2010 | 20005679 |
| a live oral recombinant salmonella enterica serovar typhimurium vaccine expressing clostridium perfringens antigens confers protection against necrotic enteritis in broiler chickens. | necrotic enteritis (ne) in broiler chickens is caused by clostridium perfringens, and there is currently no effective vaccine for ne. we previously showed that in broiler chickens protection against ne can be achieved through intramuscular immunization with alpha toxin (at) and hypothetical protein (hp), and we subsequently identified b-cell epitopes in hp. in the present study, we identified b-cell epitopes in at recognized by chickens immune to ne. the gene fragments encoding immunodominant ep ... | 2010 | 20007363 |
| resistance development profiling of piperacillin in combination with the novel {beta}-lactamase inhibitor bli-489. | to evaluate development of resistance to the piperacillin/bli-489 combination. | 2010 | 20008048 |
| identification of conserved amino acid residues of the salmonella sigmas chaperone crl involved in crl-sigmas interactions. | proteins that bind sigma factors typically attenuate the function of the sigma factor by restricting its access to the rna polymerase (rnap) core enzyme. an exception to this general rule is the crl protein that binds the stationary-phase sigma factor sigma(s) (rpos) and enhances its affinity for the rnap core enzyme, thereby increasing expression of sigma(s)-dependent genes. analyses of sequenced bacterial genomes revealed that crl is less widespread and less conserved at the sequence level tha ... | 2010 | 20008066 |
| application of a short, disordered n-terminal flagellin segment, a fully functional flagellar type iii export signal, to expression of secreted proteins. | recently, we have demonstrated that the 26-47 segment of salmonella enterica serovar typhimurium flagellin is capable of mediating flagellar export. in order to reveal whether other parts of the n-terminal region have any significant influence on secretion, a series of plasmids were constructed containing the lac promoter followed by the 26-47, 2-65, or 2-192 portion of salmonella flagellin, to which various heterologous proteins of different size were fused (18 constructs overall). essentially, ... | 2010 | 20008166 |
| regulation of salmonella enterica pathogenicity island 1 by dna adenine methylation. | dna adenine methylase (dam(-)) mutants of salmonella enterica are attenuated in the mouse model and present multiple virulence-related defects. impaired interaction of salmonella dam(-) mutants with the intestinal epithelium has been tentatively correlated with reduced secretion of pathogenicity island 1 (spi-1) effectors. in this study, we show that s. enterica dam(-) mutants contain lowered levels of the spi-1 transcriptional regulators hila, hilc, hild, and invf. epistasis analysis indicates ... | 2010 | 20008574 |
| enteric fever in mediterranean north africa. | typhoid fever is endemic in the mediterranean north african countries (morocco, algeria, tunisia, libya, and egypt) with an estimated incidence of 10-100 cases per 100,000 persons. outbreaks caused by salmonella enterica serovar typhi are common and mainly due to the consumption of untreated or sewage-contaminated water. salmonella enterica paratyphi b is more commonly involved in nosocomial cases of enteric fever in north africa than expected and leads to high mortality rates among infants with ... | 2009 | 20009276 |
| microbial alkaloid staurosporine induces formation of nanometer-wide membrane tubular extensions (cytonemes, membrane tethers) in human neutrophils. | in the present work, we demonstrate that microbial alkaloid staurosporine (sts) and ro 31-8220, structurally related to sts protein kinase c inhibitor, caused development of membrane tubular extensions in human neutrophils upon adhesion to fibronectin-coated substrata. sts-induced tubular extensions interconnected neutrophils in a network and bound serum-opsonized bacteria salmonella enterica serovar typhimurium. the diameter of sts-induced extensions varied in the range 160-200 nm. the extensio ... | 2010 | 20009568 |
| performing comparative peptidomics analyses of salmonella from different growth conditions. | host-pathogen interactions are complex competitions during which both the host and the pathogen adapt rapidly to each other in order for one or the other to survive. salmonella enterica serovar typhimurium is a pathogen with a broad host range that causes a typhoid fever-like disease in mice and severe food poisoning in humans. the murine typhoid fever is a systemic infection in which s. typhimurium evades part of the immune system by replicating inside macrophages and other cells. the transitio ... | 2010 | 20013197 |
| global trends in typhoid and paratyphoid fever. | typhoid and paratyphoid fever continue to be important causes of illness and death, particularly among children and adolescents in south-central and southeast asia, where enteric fever is associated with poor sanitation and unsafe food and water. high-quality incidence data from asia are underpinning efforts to expand access to typhoid vaccines. efforts are underway to develop vaccines that are immunogenic in infants after a single dose and that can be produced locally in countries of endemicity ... | 2010 | 20014951 |
| deficiencies in elements involved in tlr4-receptor signalling in rbl-2h3 cells. | rbl-2h3 cells express toll-like receptors, including tlr4. this study aims to assess various aspects of the tlr4 pathway. | 2010 | 20016926 |
| clonal dissemination of the multi-drug resistant salmonella enterica serovar braenderup, but not the serovar bareilly, of prevalent serogroup c1 salmonella from taiwan. | nontyphoidal salmonella is the main cause of human salmonellosis. in order to study the prevalent serogroups and serovars of clinical isolates in taiwan, 8931 salmonellae isolates were collected from 19 medical centers and district hospitals throughout the country from 2004 to 2007. the pulsed-field eletrophoresis types (pfge) and antibiotic resistance profiles of salmonella enterica serovars bareilly (s. bareilly) and braenderup (s. braenderup) were compared, and multi-drug resistance (mdr) pla ... | 2009 | 20017951 |
| transcriptional response in the peripheral blood of patients infected with salmonella enterica serovar typhi. | we used microarrays and transcriptional profiling of peripheral blood to investigate the host response of 29 individuals who contracted typhoid fever in the mekong delta region of vietnam. samples were taken over a nine month period encompassing acute disease, convalescence, and recovery. we found that typhoid fever induced a distinct and highly reproducible signature in the peripheral blood that changed during treatment and convalescence, returning in the majority of cases to the "normal" profi ... | 2009 | 20018727 |
| virulence potential of five major pathogenicity islands (spi-1 to spi-5) of salmonella enterica serovar enteritidis for chickens. | salmonella is a highly successful parasite of reptiles, birds and mammals. its ability to infect and colonise such a broad range of hosts coincided with the introduction of new genetic determinants, among them 5 major pathogenicity islands (spi1-5), into the salmonella genome. however, only limited information is available on how each of these pathogenicity islands influences the ability of salmonella to infect chickens. in this study, we therefore constructed salmonella enteritidis mutants with ... | 2009 | 20021686 |
| investigation of a community outbreak of typhoid fever associated with drinking water. | this report is about the investigation of an outbreak of typhoid fever claimed three human lives and left more than 300 people suffered within one week. the aim of this report is to draw the attention of global health community towards the areas that are still far from basic human essentialities. | 2009 | 20021691 |
| transport and distribution of salmonella enterica serovar typhimurium in loamy and sandy soil monoliths with applied liquid manure. | a leaching experiment, where liquid manure spiked with salmonella enterica serovar typhimurium (tet(+)) dsm554 was applied to soil surfaces, was conducted on intact soil monoliths (60 cm in diameter and 100 cm long). a total of 6.5 x 10(10) cfu was applied to each column. we found that salmonella serovar typhimurium could be transported to a 1-m depth in loamy soil at concentrations reaching 1.3 x 10(5) cfu/ml of leachate. the test strain was found in concentrations ranging from 300 to 1.3(5) ce ... | 2010 | 20023094 |
| identification and characterization of the aspartate chemosensory receptor of campylobacter jejuni. | campylobacter jejuni is a highly motile bacterium that responds via chemotaxis to environmental stimuli to migrate towards favourable conditions. previous in silico analysis of the c. jejuni strain nctc11168 genome sequence identified 10 open reading frames, tlp1-10, that encode putative chemosensory receptors. we describe the characterization of the role and specificity of the tlp1 chemoreceptor (cj1506c). in vitro and in vivo models were used to determine if tlp1 had a role in host colonizatio ... | 2010 | 20025667 |
| tracing lineage by phenotypic and genotypic markers in salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and salmonella typhimurium isolated in state of são paulo, brazil. | fifty-three salmonella 1,4,[5],12:i:- and 45 salmonella typhimurium strains were characterised using phage typing, plasmid profiles and pulsed-field gel electrophoresis (pfge) for comparison. the majority of the strains were subdivided into definitive type (dt) 41 (22.6%) and dt 193 (18%) and the 60-mda plasmid was detected in 94.3% and 84.4% of strains, respectively. genetic diversity was observed among all strains and 90% presented a > 70% similarity through pfge analysis. these results sugges ... | 2009 | 20027476 |
| qsec mediates salmonella enterica serovar typhimurium virulence in vitro and in vivo. | the autoinducer-3 (ai-3)/epinephrine (epi)/norepinephrine (ne) interkingdom signaling system mediates chemical communication between bacteria and their mammalian hosts. the three signals are sensed by the qsec histidine kinase (hk) sensor. salmonella enterica serovar typhimurium is a pathogen that uses hks to sense its environment and regulate virulence. salmonella serovar typhimurium invades epithelial cells and survives within macrophages. invasion of epithelial cells is mediated by the type i ... | 2010 | 20028809 |
| combined ramr mutation and presence of a tn1721-associated tet(a) variant in a clinical isolate of salmonella enterica serovar hadar resistant to tigecycline. | a salmonella enterica serovar hadar strain resistant to tigecycline (mic, 16 microg/ml) was isolated. molecular characterization revealed the presence of a plasmid-borne tet(a) variant associated with tn1721 mediating a rise of the mic for tigecycline when transferred to escherichia coli. additionally, a truncating mutation in ramr was detected. transformation with wild-type ramr but not with the mutated ramr lowered the mic for tigecycline. characterization of this salmonella isolate implicates ... | 2010 | 20028818 |
| morphology of salmonella typhimurium typing phages of the lilleengen set. | the lilleengen scheme for typing salmonella enterica serovar typhimurium consists of 12 tailed phages. ten phages are podoviruses and morphologically identical to salmonella phage p22. two phages are siphoviruses and identical to flagella-specific phage chi. | 2009 | 20029533 |
| ceftiofur resistance in salmonella enterica serovar heidelberg from chicken meat and humans, canada. | the canadian integrated program for antimicrobial resistance surveillance describes a strong correlation (r = 0.9, p<0.0001) between ceftiofur-resistant salmonella enterica serovar heidelberg isolated from retail chicken and incidence of ceftiofur-resistant salmonella serovar heidelberg infections in humans across canada. in quebec, changes of ceftiofur resistance in chicken salmonella heidelberg and escherichia coli isolates appear related to changing levels of ceftiofur use in hatcheries durin ... | 2010 | 20031042 |
| the designer proline-rich antibacterial peptide a3-apo is effective against systemic escherichia coli infections in different mouse models. | antimicrobial peptides are considered to be viable alternatives to conventional antibiotics. however, they rarely show systemic efficacy in animal models when added at non-toxic doses. the dimer a3-apo was designed to attack both the bacterial membrane and the enterobacteriaceae-specific domain of the heat shock protein dnak in order to reduce toxicity whilst maintaining activity. the peptide exhibited a minimal inhibitory concentration (mic) range of 2-128 mg/l against 28 clinical escherichia c ... | 2010 | 20031377 |
| molecular characterization of extended-spectrum cephalosporinase-producing salmonella enterica serovar choleraesuis isolates from patients in thailand and denmark. | the objective of this study was to characterize extended-spectrum cephalosporinase (esc)-producing isolates of salmonella enterica serovar choleraesuis recovered from patients in thailand and denmark. twenty-four blood culture isolates from 22 patients were included in the study, of which 23 isolates were recovered from 21 thai patients during 2003, 2007, or 2008 and one isolate was recovered from a danish traveler to thailand. esc production was confirmed in 13 out of the 24 isolates by mic tes ... | 2010 | 20032253 |
| construction and immunogenicity of salmonella vaccine vector expressing hiv-1 antigen and mcp3. | this study aims to determine the efficacy of salmonella enterica serovar typhimurium stm-1 bearing mcp-3 gene as a delivery vehicle for the hiv gag gene (in particular p24 gene) and hiv env gene. the stm1 delivery hiv-p24 vaccination was carried out in the form of a recombinant or a dna vaccine whereas only a dna vaccine was used for hiv env . naked dna vaccination was also tested and immune responses were evaluated following immunisation in mouse model. results: vaccination cellular immune resp ... | 2009 | 20038492 |
| virulotyping and antimicrobial resistance typing of salmonella enterica serovars relevant to human health in europe. | the combination of virulence gene and antimicrobial resistance gene typing using dna arrays is a recently developed genomics-based approach to bacterial molecular epidemiology. we have now applied this technology to 523 salmonella enterica subsp. enterica strains collected from various host sources and public health and veterinary institutes across nine european countries. the strain set included the five predominant salmonella serovars isolated in europe (enteritidis, typhimurium, infantis, vir ... | 2010 | 20039795 |
| detection and characterization of extended-spectrum beta-lactamase (tem-52)-producing salmonella serotype infantis from broilers in japan. | during 2004 and 2006, multidrug-resistant salmonella enterica subspecies enterica serovar infantis (salmonella infantis) isolates (n = 120) were recovered from broiler cecal samples collected from a meat-processing plant, and the isolates were examined. the study was conducted to detect and characterize extended-spectrum beta-lactamase (esbl)-producing salmonella infantis isolates recovered from broiler chickens and determine the mechanisms of transfer of the resistance traits. extended-spectrum ... | 2010 | 20039796 |
| espm2 is a rhoa guanine nucleotide exchange factor. | we investigated how the type iii secretion system wxxxe effectors espm2 of enterohaemorrhagic escherichia coli, which triggers stress fibre formation, and sifa of salmonella enterica serovar typhimurium, which is involved in intracellular survival, modulate rho gtpases. we identified a direct interaction between espm2 or sifa and nucleotide-free rhoa. nuclear magnetic resonance spectroscopy revealed that espm2 has a similar fold to sifa and the guanine nucleotide exchange factor (gef) effector s ... | 2010 | 20039879 |
| successful treatment of refractory septic arthritis caused by salmonella and staphylococcus aureus with preservation of graft function in a long-term renal transplant recipient by total withdrawal of immunosuppressants. | an 18-year-old female had received a 2 hla incompatible renal transplant 10 years before. she initially presented with septic arthritis and osteomyelitis caused by salmonella enterica co-infected with staphylococcus aureus of her left knee with development of secondary septic arthritis of the right knee and left shoulder. this was complicated by a recurrent subcutaneous abscess and intermittent high fever. the infection was successfully treated with a combination of a prolonged course of antibio ... | 2010 | 20040356 |
| prevalence and risk factors for salmonella enterica subsp. enterica contamination in french breeding and fattening turkey flocks at the end of the rearing period. | an epidemiological study was conducted to estimate the prevalence of salmonella spp. contamination in french commercial breeding and fattening turkey flocks at the end of the rearing period, as part of a european union-wide baseline study. two hundred and five breeding turkey flocks and three hundred and two fattening turkey flocks were included in the study, between october 2006 and september 2007. the salmonella status of flocks was assessed by collecting five environmental faeces samples, ana ... | 2010 | 20044159 |
| structure and mechanisms of a protein-based organelle in escherichia coli. | many bacterial cells contain proteinaceous microcompartments that act as simple organelles by sequestering specific metabolic processes involving volatile or toxic metabolites. here we report the three-dimensional (3d) crystal structures, with resolutions between 1.65 and 2.5 angstroms, of the four homologous proteins (euts, eutl, eutk, and eutm) that are thought to be the major shell constituents of a functionally complex ethanolamine utilization (eut) microcompartment. the eut microcompartment ... | 2010 | 20044574 |
| deletion of penicillin-binding protein 5 (pbp5) sensitises escherichia coli cells to beta-lactam agents. | escherichia coli penicillin-binding protein 5 (pbp5), a dd-carboxypeptidase encoded by the daca gene, plays a key role in the maintenance of cell shape. although pbp5 shares one of the highest copy numbers among the pbps, it is not essential for cell survival. to determine the effect of this redundant pbp on beta-lactam antibiotic susceptibility, pbp5 was deleted from o-antigen-negative e. coli k-12 (cs109) and o8-antigen-positive e. coli 2443, thus creating strains am15-1 and ag1o5-1, respectiv ... | 2010 | 20047819 |
| changes in the proteome of salmonella enterica serovar thompson as stress adaptation to sublethal concentrations of thymol. | thymol is a natural biocide and component of some essential oils from herbs. its inhibitory effect on the growth of different microorganisms is well documented. the precise targets of the antibacterial action of thymol is not yet been fully established, the action seems to take place in different ways. the strain salmonella enterica serovar thompson mcv1 was grown in the presence of a sublethal concentration (0.01%) of thymol. the proteins extracted from treated and untreated cells were subjecte ... | 2010 | 20049861 |
| effect of alpha-cyclodextrin-cinnamic acid inclusion complexes on populations of escherichia coli o157:h7 and salmonella enterica in fruit juices. | cinnamic acid (ca), a naturally occurring organic acid found in fruits and spices, has antimicrobial activity against spoilage and pathogenic bacteria, but low aqueous solubility limits its use. the purpose of this study was to determine the effectiveness of solubility-enhancing alpha-cyclodextrin-ca inclusion complexes against escherichia coli o157:h7 and salmonella enterica serovars suspended in apple cider or orange juice at two different incubation temperatures (4 and 26 degrees celsius). tw ... | 2010 | 20051210 |
| immunogenicity of a live recombinant salmonella enterica serovar typhimurium vaccine expressing pspa in neonates and infant mice born from naive and immunized mothers. | we are developing a salmonella vectored vaccine to prevent infant pneumonia and other diseases caused by streptococcus pneumoniae. one prerequisite for achieving this goal is to construct and evaluate new recombinant attenuated salmonella vaccine (rasv) strains suitable for use in neonates and infants. salmonella enterica serovar typhimurium strain chi9558(pya4088) specifies delivery of the pneumococcal protective antigen pspa and can protect adult mice from challenge with s. pneumoniae. this st ... | 2010 | 20053873 |
| construction of recombinant attenuated salmonella enterica serovar typhimurium vaccine vector strains for safety in newborn and infant mice. | recombinant bacterial vaccines must be safe, efficacious, and well tolerated, especially when administered to newborns and infants to prevent diseases of early childhood. many means of attenuation have been shown to render vaccine strains susceptible to host defenses or unable to colonize lymphoid tissue effectively, thus decreasing their immunogenicity. we have constructed recombinant attenuated salmonella vaccine strains that display high levels of attenuation while retaining the ability to in ... | 2010 | 20053874 |
| effects of exposure to poultry chemical decontaminants on the membrane fluidity of listeria monocytogenes and salmonella enterica strains. | there is a lack of work comparing the influence of various poultry chemical decontaminants on the membrane fluidity of pathogenic bacteria. in order to assess the possible role of physical membrane changes on bacterial adaptation to decontaminants, fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (dph) was measured in listeria monocytogenes and salmonella enterica strains before and after growth in the presence of increasing sub-lethal concentrations of decontaminants (trisodium phosphat ... | 2010 | 20056288 |
| contrasting persistence strategies in salmonella and mycobacterium. | long-term survival of persistent bacterial pathogens in mammalian hosts critically depends on their ability to avoid elimination by innate and adaptive immune responses. the persistent human pathogens that cause typhoid fever and tuberculosis exemplify alternative strategies for survival in the host: immune evasion and immune adaptation, respectively. salmonella enterica serotype typhi evades host innate immune responses and inflammation by expressing factors that interfere with its detection as ... | 2010 | 20056478 |
| association between bulk-tank milk salmonella antibody level and high calf mortality in danish dairy herds. | salmonella enterica ssp. enterica dublin is the most common salmonella serotype found in the dairy sector in denmark. salmonella antibody level in bulk-tank milk (btm), indicative of salmonella dublin infection in the herd, has been recorded regularly in all danish dairy herds through a surveillance program since 2002. the objective of this study was to investigate whether high btm salmonella antibody level was associated with high calf mortality at herd level. other risk factors for high calf m ... | 2010 | 20059928 |
| a real-time pcr method for the detection of salmonella enterica from food using a target sequence identified by comparative genomic analysis. | a 5'-nuclease real-time pcr assay using a minor groove binding probe was developed for the detection of salmonella enterica from artificially contaminated foods. s. enterica-specific sequences were identified by a comparative genomic approach. several species-specific target sequences were evaluated for specificity. a real-time pcr assay was developed targeting a nucleotide sequence within the putative type iii secretion atp synthase gene (ssan). an internal amplification control (iac) probe was ... | 2010 | 20060189 |
| emerging salmonella paratyphi a enteric fever and changing trends in antimicrobial resistance pattern of salmonella in shimla. | this retrospective study incorporates a six years, six months (january 2000-june 2006) laboratory data comprising 258 isolates of salmonella. cultures were identified by standard methods. salmonella enterica serotype typhi (s.typhi) was the more frequent serotype isolated i.e., 61.62% with the remaining 38.37% being salmonella enterica serotype paratyphi a (s. paratyphi a). there was emergence of s. paratyphi a as the predominant serotype in 2003-2004 with resurgence of serotype typhi thereon. a ... | 2010 | 20061765 |
| like will to like: abundances of closely related species can predict susceptibility to intestinal colonization by pathogenic and commensal bacteria. | the intestinal ecosystem is formed by a complex, yet highly characteristic microbial community. the parameters defining whether this community permits invasion of a new bacterial species are unclear. in particular, inhibition of enteropathogen infection by the gut microbiota ( = colonization resistance) is poorly understood. to analyze the mechanisms of microbiota-mediated protection from salmonella enterica induced enterocolitis, we used a mouse infection model and large scale high-throughput p ... | 2010 | 20062525 |
| consumption of groundwater as an independent risk factor of salmonella choleraesuis infection: a case-control study in taiwan. | infection with salmonella enterica serovar choleraesuis (s. choleraesuis) in humans can be considered as an endemic disease in certain regions of taiwan, and the number of cases has increased in recent years. the goal of the case-control study discussed in this article was to identify the possible demographic and environmental risk factors associated with s. choleraesuis infection in taiwan. during the period of december 2005 to march 2007, the case-control study was conducted in human patients ... | 2009 | 20063610 |
| [salmonella enterica and cytokine system of the host]. | contemporary data on mechanisms of immune defense during salmonellosis as well as on ability of salmonella to resist these mechanisms are presented in the review. main emphasis is made on reaction of cytokine system of innate and adaptive immunity described in experimental and clinical studies. | 2009 | 20066778 |
| the complexities of porin genetic regulation. | our understanding of porin regulation has revealed not only increasing complexity in the regulatory mechanisms, but also that the porin repertoire is more extensive than previously conceived. initially, the ompr response regulator was described as the master regulator of porin genes, but many more regulators are involved such as cpxr, phob, lrp, rob, mara, soxs, cadc, crp, fnr, toxr, h-ns, stpa, ihf, hu and leuo. in addition to micf, the first small rna (srna) that was proposed to regulate porin ... | 2010 | 20068355 |
| tracking the dynamic interplay between bacterial and host factors during pathogen-induced vacuole rupture in real time. | escape into the host cell cytosol following invasion of mammalian cells is a common strategy used by invasive pathogens. this requires membrane rupture of the vesicular or vacuolar compartment formed around the bacteria after uptake into the host cell. the mechanism of pathogen-induced disassembly of the vacuolar membrane is poorly understood. we established a novel, robust and sensitive fluorescence microscopy method that tracks the precise time point of vacuole rupture upon uptake of gram-nega ... | 2010 | 20070313 |
| molecular typing of salmonella enterica serovar sofia in australia by pulsed-field gel electrophoresis and repetitive element pcr typing. | in this study, we used two molecular fingerprinting methods to investigate the genetic and clonal relationship shared by australian salmonella sofia isolates. | 2010 | 20070444 |
| vaccination of mice with salmonella expressing vapa: mucosal and systemic th1 responses provide protection against rhodococcus equi infection. | conventional vaccines to prevent the pneumonia caused by rhodococcus equi have not been successful. we have recently demonstrated that immunization with salmonella enterica typhimurium expressing the vapa antigen protects mice against r. equi infection. we now report that oral vaccination of mice with this recombinant strain results in high and persistent fecal levels of antigen-specific iga, and specific proliferation of the spleen cells of immunized mice in response to the in vitro stimulation ... | 2010 | 20072623 |
| glucose and glucose 6-phosphate as carbon sources in extra- and intracellular growth of enteroinvasive escherichia coli and salmonella enterica. | to study the role of carbohydrates, in particular glucose, glucose 6-phosphate and mannose, as carbon substrates for extra- and intracellular replication of facultative intracellular enteric bacteria, mutants of two enteroinvasive escherichia coli (eiec) strains and a salmonella enterica serovar typhimurium isolate were constructed that were defective in the uptake of glucose and mannose (deltaptsg, manxyz), glucose 6-phosphate (deltauhpt) or all three carbohydrates (deltaptsg, manxyz, uhpt). th ... | 2010 | 20075042 |
| prevalence and characterization of salmonella gallinarum in the chicken in korea during 2000 to 2008. | salmonella enterica serovar gallinarum (salmonella gallinarum) is the causative agent of fowl typhoid (ft), a severe systemic disease of chickens that results in high mortality. since 1992, ft has become one of the most serious bacterial diseases in poultry in korea. the purpose of this study was to investigate the prevalence of ft during 2000 to 2008 and characterize the phenotype and genetic diversity of salmonella gallinarum isolates before and after adopting national-wide vaccination with a ... | 2010 | 20075274 |
| effect of lactic acid bacteria probiotic culture treatment timing on salmonella enteritidis in neonatal broilers. | in the present study, a series of experiments were conducted to evaluate the ability of a combination of 3 atcc lactobacilli (lab3) or a commercially available probiotic culture (prob) to reduce salmonella enterica serovar enteritidis (salmonella enteritidis) in broiler chicks. additionally, we varied the timing of prob administration in relationship to salmonella challenge and determined the influence on recovery of enteric salmonella. in experiments 1 to 3, chicks were randomly assigned to tre ... | 2010 | 20075275 |
| exploiting the role of tolc in pathogenicity: identification of a bacteriophage for eradication of salmonella serovars from poultry. | using a screening procedure, three bacteriophages, st27, st29, and st35, were identified with selective activity for salmonella enterica serovar typhimurium (sl1344) but not sl1344 tolc::aph. overproduction of tolc led to a lower efficiency of plating (eop), further suggesting that tolc was the target receptor. activity against other serovars of salmonella was observed but not against other species of enterobacteriaceae. this study provides proof of principle that bacteriophages can be active ag ... | 2010 | 20080996 |
| rama, a member of the arac/xyls family, influences both virulence and efflux in salmonella enterica serovar typhimurium. | the transcriptomes of salmonella enterica serovar typhimurium sl1344 lacking a functional rama or ramr or with plasmid-mediated high-level overexpression of rama were compared to those of the wild-type parental strain. inactivation of rama led to increased expression of 14 spi-1 genes and decreased expression of three spi-2 genes, and it altered expression of ribosomal biosynthetic genes and several amino acid biosynthetic pathways. furthermore, disruption of rama led to decreased survival withi ... | 2010 | 20081028 |
| interaction of bacillus species and salmonella enterica serovar typhimurium in immune or inflammatory signaling from swine intestinal epithelial cells. | previous research evaluated a laboratory strain of bacillus licheniformis (bl) in a model swine epithelium and found it exerted antiinflammatory effects on salmonella enterica serovar typhimurium (sal)-induced secretion of il-8. the current investigation evaluated the antiinflammatory actions of bacillus bacteria available commercially as feed additives for the swine industry. three isolates were obtained from the product, 2 bacillus subtilis (bs1 and bs3) and 1 bl (bl2). swine jejunal epithelia ... | 2010 | 20081082 |
| oral inoculation with salmonella enterica serovar typhimurium or choleraesuis promotes divergent responses in the somatotropic growth axis of swine. | enteric disease and immune challenge are processes that have detrimental effects on the growth performance of young swine. the current study tested the hypothesis that salmonella-induced enteric disease would perturb the endocrine growth axis in a serovar-dependent fashion. specifically, we evaluated the effects of salmonella enterica serovar typhimurium (typhimurium) and serovar choleraesuis (choleraesuis) on critical regulatory components of growth in young swine. weaned pigs were housed 2 per ... | 2010 | 20081084 |
| individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain lactobacillus johnsonii ncc933 and vaginal strain lactobacillus gasseri ks120.1 against enteric, uropathogenic and vaginosis-associated pathogens. | the mechanism underlying the killing activity of lactobacillus strains against bacterial pathogens appears to be multifactorial. here, we investigate the respective contributions of hydrogen peroxide and lactic acid in killing bacterial pathogens associated with the human vagina, urinary tract or intestine by two hydrogen peroxide-producing strains. in co-culture, the human intestinal strain lactobacillus johnsonii ncc933 and human vaginal strain lactobacillus gasseri ks120.1 strains killed ente ... | 2010 | 20082639 |
| evaluation of isotope-coded protein labeling (icpl) in the quantitative analysis of complex proteomes. | an evaluation of the icpl (isotope-coded protein labeling) non-isobaric labeling technique was performed using two different biological models. two samples containing phage t4 capsids were mixed in a 1:1 ratio after being labeled with the light or heavy versions of the icpl reagent. the analysis of this proteome demonstrated the feasibility of this approach for differential quantitative proteomics and was employed to optimize the experimental parameters of the icpl workflow. icpl-mediated analys ... | 2010 | 20082807 |
| regulation of bacterial conjugation in microaerobiosis by host-encoded functions arcab and sdhabcd. | the virulence plasmid of salmonella enterica (pslt) is an f-like conjugative plasmid. high rates of pslt transfer occur in the mammalian gut, a microaerobic environment. in this study, we describe genetic screens for host-encoded activators and repressors of the transfer operon (tra) of pslt. we show that the transcription factor arca is an activator of conjugation, especially under microaerobiosis. in turn, succinate dehydrogenase (sdhabcd) is a repressor of mating in aerobiosis. arca binds ups ... | 2010 | 20083612 |
| duplication frequency in a population of salmonella enterica rapidly approaches steady state with or without recombination. | tandem duplications are among the most common mutation events. the high loss rate of duplication suggested that the frequency of duplications in a bacterial population (1/1000) might reflect a steady state dictated by relative rates of formation (k(f)) and loss (k(l)). this possibility was tested for three genetic loci. without homologous recombination (reca), duplication loss rate dropped essentially to zero, but formation rate decreased only slightly and a steady state was still reached rapidl ... | 2010 | 20083614 |
| expression of homing receptors on iga1 and iga2 plasmablasts in blood reflects differential distribution of iga1 and iga2 in various body fluids. | although secretory iga is the most abundantly produced ig isotype, the mechanisms underlying the differential distribution of iga subclasses in various body fluids remain unclear. to explore these mechanisms, we examined the distribution of iga subclasses, the influence of the nature and sites of encounters with antigens, and the correlation between iga subclass distribution and homing potentials of circulating iga plasmablasts. iga1 predominated in serum, tears, nasal wash fluid, and saliva; th ... | 2010 | 20089794 |
| characterization of resistance genes in multidrug-resistant salmonella enterica serotype typhimurium isolated from diseased cattle in france (2002 to 2007). | we report here the results of the survey of antimicrobial resistance in 148 serotype typhimurium strains isolated from cattle in france from 2002 to 2007 and displaying more than two antimicrobial resistances. salmonella enterica serotype typhimurium of definitive phage type 104 strains that are commonly resistant to ampicillin-amoxicillin, chloramphenicol-florfenicol, streptomycin-spectinomycin, sulfonamides, and tetracycline (acssut phenotype) harbored resistance genes clustered on a complex c ... | 2010 | 20092404 |
| siderophore receptor iron is an important protective antigen against salmonella infection in chickens. | in iron-limiting environments, salmonella enterica serovar enteritidis and salmonella enterica serovar typhimurium synthesize and secrete several types of siderophore to trap trivalent ferric ions; these bacteria then express siderophore receptors called iron-regulated outer membrane proteins (iromps). in this study, we experimentally reproduced iron-limiting environments using a divalent metal chelator. iron, one of the iromps, was purified by affinity chromatography with an anti-iron-mab-immob ... | 2009 | 20095157 |
| inhibition of the early stage of salmonella enterica serovar enteritidis biofilm development on stainless steel by cell-free supernatant of a hafnia alvei culture. | compounds present in hafnia alvei cell-free culture supernatant cumulatively negatively influence the early stage of biofilm development by salmonella enterica serovar enteritidis on stainless steel while they also reduce the overall metabolic activity of s. enteritidis planktonic cells. although acylhomoserine lactones (ahls) were detected among these compounds, the use of several synthetic ahls was not able to affect the initial stage of biofilm formation by this pathogen. | 2010 | 20097823 |
| the zur-regulated zint protein is an auxiliary component of the high-affinity znuabc zinc transporter that facilitates metal recruitment during severe zinc shortage. | the pathways ensuring the efficient uptake of zinc are crucial for the ability of bacteria to multiply in the infected host. to better understand bacterial responses to zinc deficiency, we have investigated the role of the periplasmic protein zint in salmonella enterica serovar typhimurium. we have found that zint expression is regulated by zur and parallels that of znua, the periplasmic component of the zinc transporter znuabc. despite the fact that zint contributes to salmonella growth in medi ... | 2010 | 20097857 |
| evaluation of aroa deletion mutant of salmonella enterica subspecies enterica serovar abortusequi for its vaccine candidate potential. | the present study on a defined deletion aroa mutant (b-26) of salmonella enterica subspecies enterica serovar abortusequi (s. abortusequi) for residual virulence and safety in experimental model revealed that the virulence of the strain was at no difference in any of the cell assays (caprine alveolar macrophages, bovine alveolar macrophages, guinea pig blood mononuclear cells and horse blood mononuclear cells) than that of its parent virulence plasmid cured (s-787) and wild type (e-156) strains. ... | 2009 | 20099460 |
| novel cooperation experimentally evolved between species. | cooperation violates the view of "nature red in tooth and claw" that prevails in our understanding of evolution, yet examples of cooperation abound. most work has focused on maintenance of cooperation within a single species through mechanisms such as kin selection. the factors necessary for the evolutionary origin of aiding unrelated individuals such as members of another species have not been experimentally tested. here, i demonstrate that cooperation between species can be evolved in the labo ... | 2010 | 20100214 |
| characterization of a novel sialic acid transporter of the sodium solute symporter (sss) family and in vivo comparison with known bacterial sialic acid transporters. | the function of sialic acids in the biology of bacterial pathogens is reflected by the diverse range of solute transporters that can recognize these sugar acids. here, we use an escherichia colideltanant strain to characterize the function of known and proposed bacterial sialic acid transporters. we discover that the stm1128 gene from salmonella enterica serovar typhimurium, which encodes a member of the sodium solute symporter family, is able to restore growth on sialic acid to the deltanant st ... | 2010 | 20100283 |
| characterization of small cole1-like plasmids conferring kanamycin resistance in salmonella enterica subsp. enterica serovars typhimurium and newport. | multi-antibiotic resistant (mr) salmonella enterica serovars typhimurium and newport are an increasing concern in human and animal health. many strains are known to carry antibiotic resistance determinants on multiple plasmids, yet detailed information has been scarce. three plasmids conferring kanamycin (kan) resistance were isolated and nucleotide sequences were determined. two kan(r) plasmids from salmonella newport strains, psn11/00kan and psn02/01kan, were found to be identical and were 569 ... | 2010 | 20100512 |
| both hemolytic anemia and malaria parasite-specific factors increase susceptibility to nontyphoidal salmonella enterica serovar typhimurium infection in mice. | severe pediatric malaria is an important risk factor for developing disseminated infections with nontyphoidal salmonella serotypes (nts). while recent animal studies on this subject are lacking, early work suggests that an increased risk for developing systemic nts infection during malaria is caused by hemolytic anemia, which leads to reduced macrophage microbicidal activity. here we established a model for oral salmonella enterica serotype typhimurium challenge in mice infected with plasmodium ... | 2010 | 20100860 |
| recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy. | flagellin contains conserved n/c domains for tlr5 binding to activate innate immunity and a middle hypervariable domain harboring the major antigenic epitopes. however, conflict results existed in the previous studies as to whether the hypervariable domain was involved in the cytokine production and adjuvancy of flagellin. here we constructed three flagellin variants (designated as flicdelta190-278, flicdelta220-320, and flicdelta180-400) with deletions in the hypervariable domain. our data demo ... | 2010 | 20102702 |
| ndp52, a novel autophagy receptor for ubiquitin-decorated cytosolic bacteria. | autophagy functions as a cell-autonomous effector mechanism of innate immunity by separating bacteria from cytosolic resources and delivering them for lysosomal destruction. how cytosolic bacteria are targeted for autophagy is incompletely understood. we recently discovered that salmonella enterica serotype typhimurium and streptococcus pyogenes are detected by ndp52 (nuclear dot protein 52 kda), after these bacteria enter the cytosol of human cells and become decorated with polyubiquitinated pr ... | 2010 | 20104023 |
| empyema of preexisting subdural hemorrhage caused by a rare salmonella species after exposure to bearded dragons in a foster home. | an infant had a subdural empyema caused by the rare salmonella species enterica subspecies houtenae (iv) serotype 44:z4,z23:- after only indirect exposure to exotic reptiles in her foster home. infants recovering from preexisting subdural hematoma are at risk for development of empyema. | 2010 | 20105642 |
| prime-boost immunization using a dna vaccine delivered by attenuated salmonella enterica serovar typhimurium and a killed vaccine completely protects chickens from h5n1 highly pathogenic avian influenza virus. | h5n1 highly pathogenic avian influenza virus (hpaiv) has posed a great threat not only for the poultry industry but also for human health. however, an effective vaccine to provide a full spectrum of protection is lacking in the poultry field. in the current study, a novel prime-boost vaccination strategy against h5n1 hpaiv was developed: chickens were first orally immunized with a hemagglutinin (ha) dna vaccine delivered by attenuated salmonella enterica serovar typhimurium, and boosting with a ... | 2010 | 20107004 |
| development of a bead immunoassay to measure vi polysaccharide-specific serum igg after vaccination with the salmonella enterica serovar typhi vi polysaccharide. | vi polysaccharide from salmonella enterica serotype typhi is used as one of the available vaccines to prevent typhoid fever. measurement of vi-specific serum antibodies after vaccination with vi polysaccharide by enzyme-linked immunosorbent assay (elisa) may be complicated due to poor binding of the vi polysaccharide to elisa plates resulting in poor reproducibility of measured antibody responses. we chemically conjugated vi polysaccharide to fluorescent beads and performed studies to determine ... | 2010 | 20107010 |
| development and evaluation of a multiplex real-time polymerase chain reaction procedure to clinically type prevalent salmonella enterica serovars. | a multiplex real-time polymerase chain reaction procedure was developed to identify the most prevalent clinical isolates of salmonella enterica subsp. enterica. genes from the rfb, flic, fljb, and viab groups that encode the o, h, and vi antigens were used to design 15 primer pairs and taqman probes specific for the genes rfbj, wzx, flic, fljb, wcdb, the sdf-l sequence, and inva, which was used as an internal amplification control. the primers and probes were variously combined into six sets. th ... | 2010 | 20110454 |
| interaction of saccharomyces boulardii with salmonella enterica serovar typhimurium protects mice and modifies t84 cell response to the infection. | salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment of cellular small gtp-binding proteins induced by the bacteria, and by triggering a pro-inflammatory response through activation of mapks and nuclear translocation of nf-kappab. worldwide interest in the use of functional foods containing probiotic bacteria for health promotion and disease prevention has increased significantly. saccharomyces boulardii is a non-pathogenic yeast ... | 2010 | 20111723 |
| [salmonella choleraesuis infection in children: chinical analysis of 21 cases]. | | 2009 | 20113613 |
| comparison of a semi-automated rep-pcr system and multilocus sequence typing for differentiation of salmonella enterica isolates. | the accurate sub-typing of salmonella enterica isolates is essential for epidemiological investigations and surveillance of salmonella infections. salmonella isolates are currently identified using the kauffman-white serotyping scheme. multilocus sequence typing (mlst) schemes have been developed for the major bacterial pathogens including salmonella and have assisted in understanding the molecular epidemiology and population biology of these organisms. recently, the diversilab rep-pcr system ha ... | 2010 | 20114063 |