| the vibrio parahaemolyticus small rna ryhb promotes production of the siderophore vibrioferrin by stabilizing the polycistronic mrna. | high-affinity iron acquisition in vibrio parahaemolyticus is mediated by the cognate siderophore vibrioferrin. we have previously reported that the vibrioferrin biosynthesis operon (pvsop) is regulated at the transcriptional level by the iron-responsive repressor fur (t. tanabe, t. funahashi, h. nakao, s. miyoshi, s. shinoda, and s. yamamoto, j. bacteriol. 185:6938-6949, 2003). in this study, we identified the fur-regulated small rna ryhb and the rna chaperone hfq protein as additional regulator ... | 2013 | 23772063 |
| biosynthesis of the osmoprotectant ectoine, but not glycine betaine, is critical for survival of osmotically stressed vibrio parahaemolyticus cells. | vibrio parahaemolyticus is a halophile present in marine and estuarine environments, ecosystems characterized by fluctuations in salinity and temperature. one strategy to thrive in such environments is the synthesis and/or uptake of compatible solutes. the v. parahaemolyticus genome contains biosynthesis systems for both ectoine and glycine betaine, which are known to act as compatible solutes in other species. we showed that v. parahaemolyticus had a 6% nacl tolerance when grown in m9 minimal m ... | 2013 | 23770911 |
| mechanisms of fluoroquinolone resistance in vibrio parahaemolyticus. | | 2013 | 23751355 |
| genome sequence analysis of the vibrio parahaemolyticus lytic bacteriophage vpms1. | vpms1 is a vibrio parahaemolyticus lytic phage isolated from a marine clam. the 42.3-kb genome was predicted to encode 53 proteins. comparison of the vpms1 dna genome with known phage genomes revealed no similarity; hence, it represents a new vp phage, organized into three differently oriented modules. the module for packaging covers 12 % of the genome, the module for structure covers 31 %, and the module for replication and regulation covers 48 %. the g + c content was 44.67 %. the coding regio ... | 2013 | 23732928 |
| molecular characterization of a p38 mapk from litopenaeus vannamei and its expression during the molt cycle and following pathogen infection. | the p38 mitogen-activated protein kinase (mapk), a serine/threonine-specific protein kinase, has been reported to be involved in innate immunity, development and muscle differentiation. to explore the function of p38 in shrimp, partial cdna sequence of p38 in litopenaeus vannamei (designated as lv-p38) was characterized and the expression of lv-p38 in hepatopancreas of the shrimp after being infected with vibrio parahaemolyticus and in muscle of the shrimp at different molt stages was detected b ... | 2013 | 23707783 |
| molecular epidemiology and genetic variation of pathogenic vibrio parahaemolyticus in peru. | vibrio parahaemolyticus is a foodborne pathogen that has become a public health concern at the global scale. the epidemiological significance of v. parahaemolyticus infections in latin america received little attention until the winter of 1997 when cases related to the pandemic clone were detected in the region, changing the epidemic dynamics of this pathogen in peru. with the aim to assess the impact of the arrival of the pandemic clone on local populations of pathogenic v. parahaemolyticus in ... | 2013 | 23696906 |
| the iodide-transport-defect-causing mutation r124h: a δ-amino group at position 124 is critical for maturation and trafficking of the na+/i- symporter. | na(+)/i(-) symporter (nis)-mediated active accumulation of i(-) in thyrocytes is a key step in the biosynthesis of the iodine-containing thyroid hormones t3 and t4. several nis mutants have been identified as a cause of congenital i(-) transport defect (itd), and their investigation has yielded valuable mechanistic information on nis. here we report novel findings derived from the thorough characterization of the itd-causing mutation r124h, located in the second intracellular loop (il-2). r124h ... | 2013 | 23690546 |
| molecular characterization of clinical and environmental vibrio parahaemolyticus isolates in taiwan. | vibrio parahaemolyticus is the most prevalent foodborne pathogen in taiwan and it is frequently recovered from seafood. in this study, v. parahaemolyticus that was isolated in recent years from aquacultural environments and clinical specimens were comparatively analyzed by noti-restricted pulsed-field gel electrophoresis (pfge) and polymerase chain reaction, targeting common toxin genes (tdh, trh, urec), mtase gene, toxr regulator, markers for pandemic strains (orf8, group-specific toxrs) and re ... | 2013 | 23685468 |
| a pentaplex pcr assay for detection and characterization of vibrio vulnificus and vibrio parahaemolyticus isolates. | vibrio parahaemolyticus and vibrio vulnificus are the leading causes of seafood-related illnesses and also can cause wound infections. these bacteria often co-exist in marine and estuarine environments. however, there have been no reported protocols that can detect and characterize (i.e. pathogenic or nonpathogenic) them in a single pcr. in this study, we developed a ppcr assay with a combination of two species-specific and three pathogenic-specific pcr primers to simultaneously detect virulent ... | 2013 | 23682646 |
| [selection of reference genes for virulence gene expression in vibrio parahaemolyticus]. | we selected reference genes for virulence gene expression of vibrio parahaemolyticus under different environmental conditions. | 2013 | 23678578 |
| molecular insights of the first gastropod tlr counterpart from disk abalone (haliotis discus discus), revealing its transcriptional modulation under pathogenic stress. | toll-like receptors (tlrs) are well-characterized pattern recognition receptors of innate immunity, known to induce immune responses against the pathogens by interacting with evolutionarily conserved pathogen-associated molecular patterns (pamps). in this study, a novel tlr homolog from disk abalone (haliotis discus discus) was identified and characterized at molecular level. the open reading frame (orf) of abtlr is 3804 bp in length and encodes a 1268 amino acid peptide with a calculated molecu ... | 2013 | 23669649 |
| expression of glutamine synthetase in tegillarca granosa (bivalvia, arcidae) hemocytes stimulated by vibrio parahaemolyticus and lipopolysaccharides. | the blood cockle, tegillarca granosa, is a widely consumed clam in the indo-pacific region. glutamine synthetase (gs) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. we identified the gs of t. granosa (tg-gs) from hemocytes by 3'- and 5'-rapid amplification of cdna ends (race)-pcr. the full-length cdna consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. sequence compar ... | 2013 | 23661439 |
| multiplex pcr for the detection and differentiation of vibrio parahaemolyticus strains using the groel, tdh and trh genes. | vibrio parahaemolyticus is a significant cause of human gastrointestinal disorders worldwide, transmitted primarily by ingestion of raw or undercooked contaminated seafood. in this study, a multiplex pcr assay for the detection and differentiation of v. parahaemolyticus strains was developed using primer sets for a species-specific marker, groel, and two virulence markers, tdh and trh. multiplex pcr conditions were standardised, and extracted genomic dna of 70 v. parahaemolyticus strains was use ... | 2013 | 23660458 |
| a vibrio parahaemolyticus t3ss effector mediates pathogenesis by independently enabling intestinal colonization and inhibiting tak1 activation. | vibrio parahaemolyticus type iii secretion system 2 (t3ss2) is essential for the organism's virulence, but the effectors required for intestinal colonization and induction of diarrhea by this pathogen have not been identified. here, we identify a type iii secretion system (t3ss2)-secreted effector, vopz, that is essential for v. parahaemolyticus pathogenicity. vopz plays distinct, genetically separable roles in enabling intestinal colonization and diarrheagenesis. truncation of vopz prevents v. ... | 2013 | 23623501 |
| [inactivation kinetics of vibrio parahaemolyticus in peeled shrimp treated with organic acids]. | to establish the kinetic inactivation models of vibrio parahaemolytius in saline and peeled shrimp treated with acetic acid, lactic acid and citric acid for guidance of their potential application in shrimp decontamination. | 2013 | 23614238 |
| vibrio parahaemolyticus type vi secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. | vibrio parahaemolyticus is a marine bacterium that thrives in warm climates. it is a leading cause of gastroenteritis resulting from consumption of contaminated uncooked shellfish. this bacterium harbors two putative type vi secretion systems (t6ss). t6sss are widespread protein secretion systems found in many gram-negative bacteria, and are often tightly regulated. for many t6sss studied to date, the conditions and cues, as well as the regulatory mechanisms that control t6ss activity are unknow ... | 2013 | 23613791 |
| gating of the trkh ion channel by its associated rck protein trka. | trkh belongs to a superfamily of k(+) transport proteins required for growth of bacteria in low external k(+) concentrations. the crystal structure of trkh from vibrio parahaemolyticus showed that trkh resembles a k(+) channel and may have a gating mechanism substantially different from k(+) channels. trkh assembles with trka, a cytosolic protein comprising two rck (regulate the conductance of k(+)) domains, which are found in certain k(+) channels and control their gating. however, fundamental ... | 2013 | 23598339 |
| murine macrophage inflammatory cytokine production and immune activation in response to vibrio parahaemolyticus infection. | vibrio parahaemolyticus is the most common cause of bacterial, seafood-related illness in the usa. currently, there is a dearth of published reports regarding immunity to infection with this pathogen. here, production of both pro- and anti-inflammatory cytokines by v. parahaemolyticus-infected raw 264.7 murine macrophages was studied. it was determined that this infection results in increased concentrations of il-1α, il-6, tnf-α and il-10. additionally, decreases in cell surface tlr2 and tlr4 an ... | 2013 | 23586636 |
| isolation and characterization of pandemic and nonpandemic strains of vibrio parahaemolyticus from an outbreak of diarrhea in north 24 parganas, west bengal, india. | strains of the enteric pathogen vibrio parahaemolyticus harboring the thermostable hemolysin (tdh) encoding gene tdh is known to cause epidemic and pandemic diarrhea. in industrialized countries, this pathogen causes sporadic or outbreaks of diarrheal illness associated with consumption of raw or improperly cooked seafood. this report describes a foodborne outbreak of gastroenteritis caused by v. parahaemolyticus in june 2011 following consumption of food served at a funeral reception held at ha ... | 2013 | 23566271 |
| effects of pre- or post-processing storage conditions on high-hydrostatic pressure inactivation of vibrio parahaemolyticus and v. vulnificus in oysters. | the effects of storage conditions on subsequent high-hydrostatic pressure (hhp) inactivation of vibrio parahaemolyticus and vibrio vulnificus in oysters were investigated. live oysters were inoculated with v. parahaemolyticus or v. vulnificus to ca. 7-8 log mpn/g by feeding and stored at varying conditions (i.e., 21 or 35 °c for 5h, 4 or 10 °c for 1 and 2 days and -18 °c for 2 weeks). oyster meats were then treated at 225-300 mpa for 2 min at 4, 21 or 35 °c. hhp at 300 mpa for 2 min achieved a > ... | 2013 | 23545264 |
| ecological study of pathogenic vibrios in aquatic environments. | an ecological study of pathogenic vibrios in aquatic environments of okayama was carried out. the number of vibrio parahaemolyticus detected in the sea area was comparatively smaler than that found in the survey of about two decades ago. various reasons for the decrease in the case of food poisoning by v. parahaemolyticus have been suggested but the lower number of the vibrio in aquatic environments may be one explanation. although the number of v. vulnificus was also not as large, most of the i ... | 2013 | 23538851 |
| transcriptomic and cellular response to bacterial challenge (pathogenic vibrio parahaemolyticus) in farmed juvenile haliotis rufescens fed with or without probiotic diet. | the abalone production in chile has increased considerably in recent years with no sign of tapering off. open and semi-closed circuits in the marine water zones in the north and south of chile are the preferred areas of culture. coastal ecosystems are subjected to a wide variety of contaminants that generate stress that affects populations via their impacts to individuals at both physiological and genetic levels. this work investigated the genomic and cellular response of post-weaning juvenile h ... | 2013 | 23535139 |
| susceptibility of vibrio parahaemolyticus to disinfectants after prior exposure to sublethal stress. | in the present study, vibrio parahaemolyticus 690 in phosphate buffered-saline containing 3% nacl was subjected to sublethal stresses: heat shock at 42 °c for 15 min, acid adaptation at ph 5.0 for 30 min, or cold shock at 20 °c for 4 h. the effect of sublethal stress on the susceptibility of v. parahaemolyticus to a chlorine-containing disinfectant (clidox-s) and a quaternary ammonium compound (quatricide) at 25 and 40 °c was investigated. it was found that the sublethal stresses examined enhanc ... | 2013 | 23498199 |
| [isolation of vibrio parahaemolyticus having new combination of o and k serotypes from sporadic diarrheal patients]. | | 2013 | 23484380 |
| structure and immune expression analysis of hemoglobin genes from the blood clam tegillarca granosa. | hemoglobin (hb) is the major protein component of erythrocytes in animals with red blood, although it can serve additional functions beyond the transport of oxygen. the blood clam (tegillarca granosa) is one of the few mollusks that has hb, although the structure and function of molluskan hbs remain unclear. we characterized two unique and highly compartmentalized blood clam hemoglobin genes, tg-hbiia and tg-hbiib, at the molecular level. the full-length cdna of tg-hbiia was 731 bp with a 450-bp ... | 2013 | 23479150 |
| polymorphism of the multiple hemoglobins in blood clam tegillarca granosa and its association with disease resistance to vibrio parahaemolyticus. | hemoglobin (hb) is the major protein component of erythrocytes in animals with red blood, but it can serve additional functions beyond the transport of oxygen. in this study, we identified polymorphism in the blood clam tegillarca granosa hb (tg-hb) genes and investigated the association of this polymorphism with resistance/susceptibility to vibrio parahaemolyticus. analysis of the 540 sequences revealed 28 snps in the coding region of three tg-hbs, corresponding to about one snp per 48 bp. thre ... | 2013 | 23470816 |
| complete genome sequence of prepandemic vibrio parahaemolyticus bb22op. | the number of inflammatory gastroenteritis outbreaks due to the food-borne pathogen vibrio parahaemolyticus is rising sharply worldwide and in the united states in particular. here we report the complete, annotated genome sequence of the prepandemic v. parahaemolyticus strain bb22op and make some initial comparisons to the complete genome sequence for pandemic strain rimd2210633. | 2013 | 23469330 |
| rapid identification of vibrio parahaemolyticus isolated from shellfish, sea water and sediments of the khnifiss lagoon, morocco, by maldi-tof mass spectrometry. | we establish the presence of vibrio parahaemolyticus and deepen the comparison of isolates using maldi-tof ms for the typing of isolates originating from the khnifiss lagoon (morocco). amongst 48 samples from sea water, sediment and shellfish isolated from different sites of khnifiss lagoon, morocco, we obtained 22 isolates of v. parahaemolyticus identified by vitek 2™ system (biomérieux) and maldi biotyper™ (bruker daltonics). all isolates were highly resistant to ampicillin and ticarcillin, mo ... | 2013 | 23464928 |
| in silico analysis of putative paralytic shellfish poisoning toxins export proteins in cyanobacteria. | paralytic shellfish poisoning toxins (psts) are a family of more than 30 natural alkaloids synthesized by dinoflagellates and cyanobacteria whose toxicity in animals is mediated by voltage-gated na(+) channel blocking. the export of pst analogues may be through sxtf and sxtm, two putative mate (multidrug and toxic compound extrusion) family transporters encoded in psts biosynthetic gene cluster (sxt). sxtm is present in every sxt cluster analyzed; however, sxtf is only present in the cylindrospe ... | 2013 | 23457475 |
| isolation of pandemic vibrio parahaemolyticus from uk water and shellfish produce. | vibrio parahaemolyticus is a gram-negative, halophilic bacterium found commonly in temperate and warm estuarine waters worldwide. v. parahaemolyticus is considered an emerging bacterial pathogen in europe and has been responsible for several recent seafood-associated outbreaks. during ad hoc testing of raw shellfish produce in may 2012, pandemic group (o3:k6) v. parahaemolyticus was isolated from pacific oysters (crassostrea gigas), harvested in southern england. follow-on testing of water and s ... | 2013 | 23455432 |
| vibrio parahaemolyticus type iv pili mediate interactions with diatom-derived chitin and point to an unexplored mechanism of environmental persistence. | vibrio parahaemolyticus is a naturally occurring bacterium common in coastal waters where it concentrates in shellfish through filter feeding. the bacterium is a human pathogen and the leading cause of seafood-borne gastroenteritis. presently there is little information regarding mechanisms of environmental persistence of v.parahaemolyticus or an accurate early warning system for outbreak prediction. vibrios have been shown to adhere to several substrates in the environment, including chitin, on ... | 2013 | 23441888 |
| virulence determinants for vibrio parahaemolyticus infection. | vibrio parahaemolyticus is a marine microorganism that causes acute gastroenteritis associated with the consumption of contaminated raw or under cooked seafood. during infection, the bacterium utilizes a wide variety of virulence factors, including adhesins, toxins and type iii secretion systems, to cause both cytotoxicity in cultured cells and enterotoxicity in animal models. herein, we describe recent discoveries on the regulation and characterization of the virulence factors from v. para. det ... | 2013 | 23433802 |
| presence of pathogenic vibrio parahaemolyticus in waters and seafood from the tunisian sea. | the occurrence of the hemolysin genes, tdh and trh, in vibrio parahaemolyticus strains isolated from environmental samples collected from various exported seafood products comprising of fishes and shellfish (mytilus edulis and crassostrea gigas) or seawater, was studied. eight strains were confirmed as v. parahaemolyticus by toxr -based polymerase chain reaction and only one strain out of these 8 strains was positive for tdh and trh genes. toxigenic v. parahaemolyticus isolates are present in tu ... | 2013 | 23430717 |
| population structure of clinical and environmental vibrio parahaemolyticus from the pacific northwest coast of the united states. | vibrio parahaemolyticus is a common marine bacterium and a leading cause of seafood-borne bacterial gastroenteritis worldwide. although this bacterium has been the subject of much research, the population structure of cold-water populations remains largely undescribed. we present a broad phylogenetic analysis of clinical and environmental v. parahaemolyticus originating largely from the pacific northwest coast of the united states. repetitive extragenic palindromic pcr (rep-pcr) separated 167 is ... | 2013 | 23409028 |
| draft genome sequence of vibrio parahaemolyticus snuvps-1 isolated from korean seafood. | vibrio parahaemolyticus is the leading cause of food-borne diseases, and several pathogenic strains cause global gastroenteritis outbreaks. here, we report a draft genome sequence of v. parahaemolyticus snuvps-1, which was isolated from seafood in a fishery market in the republic of korea and contained tl, toxr, and toxrs(old) genes. the current draft genome sequence will contribute to the effort to monitor the spread of v. parahaemolyticus seafood isolates and clinical isolates. | 2013 | 23405312 |
| uptake and retention of vibrio parahaemolyticus in a cohabitating population of ruditapes decussatus and ruditapes philippinarum under experimental conditions. | the presence of vibrio parahaemolyticus in bivalve mollusc is an important cause of foodborne illnesses, and their levels are influenced by environmental changes, such as temperature and salinity. clams are common species in estuaries and are used in environmental monitoring programmes. present study compared the uptake and retention of nonpathogenic v. parahaemolyticus by two species of clam (ruditapes decussatus and r. philippinarum), cohabitating in a closed system. results showed no signific ... | 2013 | 23397223 |
| a candidate ion-retaining state in the inward-facing conformation of sodium/galactose symporter: clues from atomistic simulations. | the recent vibrio parahaemolyticus sodium/galactose (vsglt) symporter crystal structure captures the protein in an inward-facing substrate-bound conformation, with the sodium ion placed, by structural alignment, in a site equivalent to the na2 site of the leucine transporter (leut). a recent study, based on molecular dynamics simulations, showed that the sodium ion spontaneously leaves its initial position diffusing outside vsglt, toward the intracellular space. this suggested that the crystal s ... | 2013 | 26588767 |
| genetic relationships of vibrio parahaemolyticus isolates from clinical, human carrier, and environmental sources in thailand, determined by multilocus sequence analysis. | vibrio parahaemolyticus is a seafood-borne pathogenic bacterium that is a major cause of gastroenteritis worldwide. we investigated the genetic and evolutionary relationships of 101 v. parahaemolyticus isolates originating from clinical, human carrier, and various environmental and seafood production sources in thailand using multilocus sequence analysis. the isolates were recovered from clinical samples (n = 15), healthy human carriers (n = 18), various types of fresh seafood (n = 18), frozen s ... | 2013 | 23377932 |
| in situ strain-level detection and identification of vibrio parahaemolyticus using surface-enhanced raman spectroscopy. | the outer membrane of a bacterium is composed of chemical and biological components that carry specific molecular information related to strains, growth stages, expressions to stimulation, and maybe even geographic differences. in this work, we demonstrate that the biochemical information embedded in the outer membrane can be used for rapid detection and identification of pathogenic bacteria using surface-enhanced raman spectroscopy (sers). we used seven different strains of the marine pathogen ... | 2013 | 23356387 |
| high frequency of virulence factor genes tdh, trh, and tlh in vibrio parahaemolyticus strains isolated from a pristine estuary. | virulence factor genes encoding the thermostable direct hemolysin (tdh) and the thermostable direct hemolysin-related hemolysin (trh) are strongly correlated with virulence of the emergent human pathogen vibrio parahaemolyticus. the gene encoding the thermolabile hemolysin (tlh) is also considered a signature molecular marker for the species. these genes are typically reported in very low percentages (1 to 2%) of nonclinical strains. v. parahaemolyticus strains were isolated from various niches ... | 2013 | 23354697 |
| inactivation of vibrio parahaemolyticus in hard clams (mercanaria mercanaria) by high hydrostatic pressure (hhp) and the effect of hhp on the physical characteristics of hard clam meat. | shellfish may internalize dangerous pathogens during filter feeding. traditional methods of depuration have been found ineffective against certain pathogens. the objective was to explore high hydrostatic pressure (hhp) as an alternative to the traditional depuration process. the effect of hhp on the survival of vibrio parahaemolyticus in live clams (mercanaria mercanaria) and the impact of hhp on physical characteristics of clam meat were investigated. clams were inoculated with up to 7 log cfu/ ... | 2013 | 23324022 |
| preliminary study of transplanting as a process for reducing levels of vibrio vulnificus and vibrio parahaemolyticus in shellstock oysters. | increasingly strict standards for harvest of oysters for the raw, half-shell market (designated as "white tag") should increase the proportion of oysters not meeting these standards (designated as "green tag"). transplanting of green tag oysters into highsalinity waters (>20 practical salinity units) was explored as a means of returning vibrio vulnificus and vibrio parahaemolyticus levels to levels present on initial harvest. in summer 2011, oysters originally harvested in louisiana were transpl ... | 2013 | 23317866 |
| effects of the dietary administration of sodium alginate on the immune responses and disease resistance of taiwan abalone, haliotis diversicolor supertexta. | sodium alginate extracted from brown algae was reported to enhance the immune response and resistance of fish and shrimp. in this study, survival rates of the abalone, haliotis diversicolor supertexta, against vibrio parahaemolyticus, and its non-specific immune parameters such as the total haemocyte count (thc), phenoloxidase (po) activity, respiratory bursts, superoxide dismutase (sod) activity, phagocytic activity, and clearance efficiency to v. parahaemolyticus by h. diversicolor supertexta ... | 2013 | 23296117 |
| decontamination of vibrio parahaemolyticus in fish by washing with hygienic seawater and impacts of the high level contamination in the gills and viscera. | the effect of washing in vibrio parahaemolyticus contaminated and hygienic seawater on fish, and the frequency and level of natural v. parahaemolyticus contamination in fish were investigated. in the first experiment, live horse mackerel was experimentally kept in seawater artificially contaminated with v. parahaemolyticus. after washing in contaminated and hygienic seawater, the contamination in fish was quantitatively analyzed. washing fish in the seawater contaminated with v. parahaemolyticus ... | 2013 | 23292104 |
| changes of ultrastructure and stress tolerance of vibrio parahaemolyticus upon entering viable but nonculturable state. | this study examined the change of ultrastructure and stress tolerance of the marine foodborne pathogen, vibrio parahaemolyticus 1137, when incubated under viable but nonculturable (vbnc) state induction conditions for different time intervals. the rod-shaped v. parahaemolyticus cells in the exponential phase became coccoid cells in the vbnc state, with aberrantly shaped cells formed in the initial stage. in the aberrantly shaped cells, the cell wall was loosened, flexible and allowed the cell to ... | 2013 | 23290246 |
| prevalence of vibrio parahaemolyticus in oyster and clam culturing environments in taiwan. | vibrio parahaemolyticus is the most prevalent gastroenteritis pathogen in taiwan and some other asian countries, and it frequently occurs in oysters and other seafood. this study monitors changes in the density of v. parahaemolyticus and environmental parameters in oyster and hard clam aquacultural environments in taiwan. water, sediment and shellfish samples were collected from five sampling sites in 2008-2010, and analyzed for environmental physiochemical parameters, numbers of indicator bacte ... | 2013 | 23290223 |
| [effects of ultra-high pressure on membrane components in vibrio parahaemolyticus]. | we observed the changes in cell membrane of vibrio parahaemolyticus which is a prevalent foodborne pathogen by high pressure treatments. | 2012 | 23289323 |
| detection of vibrio parahaemolyticus in food samples using in situ loop-mediated isothermal amplification method. | a novel in situ loop-mediated isothermal amplification (in situ lamp) technique for rapid detection of the food-borne vibrio parahaemolyticus strains had been developed and evaluated in this study. the sensitivity of the in situ lamp assay was detected to be 10 cfu/reaction via test in serial 10-fold dilutions of v. parahaemolyticus cells, and high specificity had also been obtained through confirmation with 14 reference gram-positive and -negative strains. application of the established in situ ... | 2013 | 23266627 |
| development and validation of a predictive model for the growth of vibrio parahaemolyticus in post-harvest shellstock oysters. | information is limited about the growth and survival of naturally-occurring vibrio parahaemolyticus in live oysters under commercially relevant storage conditions harvested from different regions and in different oyster species. this study produced a predictive model for the growth of naturally-occurring v. parahaemolyticus in live eastern oysters (crassostrea virginica) harvested from the chesapeake bay, md, usa and stored at 5-30 °c until oysters gapped. the model was validated with model-inde ... | 2013 | 23246606 |
| rapid identification and differentiation of vibrio parahaemolyticus from vibrio spp. in seafood samples using developed monoclonal antibodies. | monoclonal antibodies (mabs) specific to vibrio parahaemolyticus were successfully generated. according to the specificity of v. parahaemolyticus, mabs can be classified into 5 groups. the mabs vp-2d and vp-11h were specific to the o2 and o4 groups of v. parahaemolyticus, respectively. the mab vp-11b reacted with 11 out of 30 isolates of v. parahaemolyticus used in this study. the mab vp-516 bound to 27 out of 30 isolates of v. parahaemolyticus and cross reacted with all 10 isolates of v. algino ... | 2013 | 23233121 |
| [vpa1045 and vpa1049 of vibrio parahaemolyticus regulate translocation of hcp2]. | vibrio parahaemolyticus causes acute gastroenteritis by secreting a number of virulence factors including two sets of type vi secretion systems (t6ss1 and t6ss2). however, it remains unknown how t6ss is regulated in the bacterium and what kinds of effects the systems have on adhesion onto and invasion into the host cells. this study was attempted to examine if vpa1045 and vpa1049 regulate t6ss2. | 2012 | 23173431 |
| genome sequence of temperate vibrio parahaemolyticus bacteriophage vb_vpas_mar10. | vibrio parahaemolyticus is recognized as one of the main causes of human gastroenteritis associated with seafood. we have fully sequenced the genome of a newly isolated phage, vb_vpas_mar10, which lysed 61.9% of the v. parahaemolyticus strains tested. phage mar10 is a temperate siphovirus, and its genome consists of double-stranded dna (dsdna) with a size of 78,751 bp, a g+c content of 49.70%, and 104 open reading frames. bioinformatic analysis shows that phage mar10 is closely related to vibrio ... | 2012 | 23166255 |
| [study of possibility of formation of vibrio parahaemolyticus pandemic clones based on retrospective pcr-screening of clinical strains]. | pcr-genotyping of vibrio parahaemolyticus strains that had caused sporadic diseases in novorossiysk from 1973 to 1976. | 2012 | 23163032 |
| characterization of the type iii secretion associated low calcium response genes of vibrio parahaemolyticus rimd2210633. | vibrio parahaemolyticus is a significant human pathogen associated with gastroenteritis. two v. parahaemolyticus type 3 secretion systems, t3ss-1 and t3ss-2, secrete effector proteins and have been implicated in host-cell cytotoxicity and enterotoxicity, respectively. vibrio parahaemolyticus t3ss-1 substrates have been identified, although many predicted substrates (based on homologies) remain undetected in secreted fractions and therefore uncharacterized. we have experimentally developed and op ... | 2012 | 23145828 |
| the role of type iii secretion system 2 in vibrio parahaemolyticus pathogenicity. | vibrio parahaemolyticus, a gram-negative marine bacterial pathogen, is emerging as a major cause of food-borne illnesses worldwide due to the consumption of raw seafood leading to diseases including gastroenteritis, wound infection, and septicemia. the bacteria utilize toxins and type iii secretion system (t3ss) to trigger virulence. t3ss is a multi-subunit needle-like apparatus used to deliver bacterial proteins, termed effectors, into the host cytoplasm which then target various eukaryotic sig ... | 2012 | 23124738 |
| complete genome sequence of vibrio parahaemolyticus bacteriophage vb_vpam_mar. | vibrio parahaemolyticus is a major pathogen that is mainly associated with seafood and is a global food safety issue. our objective was to isolate and completely sequence a specific phage against this bacterium. phage vb_vpam_mar is able to lyse 76% of the v. parahaemolyticus strains tested. mar belongs to the myoviridae family and has a genome comprised of double-stranded dna with a size of 41,351 bp, a g+c content of 51.3%, and 62 open reading frames (orfs). bioinformatic analysis showed that ... | 2012 | 23118463 |
| electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus. | a carboxyl functionalized graphene oxide (go-cooh) and electropolymerized poly-l-lysine (plly) modified glassy carbon electrode (gce) was fabricated and used for the construction of an electrochemical deoxyribonucleic acid (dna) biosensor. the nh(2) modified probe ssdna sequences were immobilized on the surface of go-cooh/plly/gce by covalent linking with the formation of amide bonds, which was stable and further hybridized with the target ssdna sequence. differential pulse voltammetry (dpv) was ... | 2012 | 23101650 |
| heterogeneous interactome between litopenaeus vannamei plasma proteins and vibrio parahaemolyticus outer membrane proteins. | a great loss has been suffered by microbial infectious diseases under intensive shrimp farming in recent years. in this background, the understanding of shrimp innate immunity becomes an importantly scientific issue, but little is known about the heterogeneous protein-protein interaction between pathogenic cells and hosts, which is a key step for the invading microbes to infect internet organs through bloodstream. in the present study, bacterial outer membrane (om) protein array and pull-down ap ... | 2013 | 23099052 |
| the role of catalase in the immune response to oxidative stress and pathogen challenge in the clam meretrix meretrix. | catalase (cat) can effectively eliminate h(2)o(2) and maintain the redox balance of immune system, which is essential for innate immunity. a catalase gene was cloned and its potential role in immune system was investigated in the clam, meretrix meretrix. the catalase (mmecat) gene had an open reading frame of 1533 bp encoding 511 amino acids which showed high identity with that of molluscs. the distribution of mmecat in clam tissues was examined and the mrna, protein expression and cat activity ... | 2013 | 23079536 |
| the in vitro and in vivo efficacy of hen igy against vibrio parahaemolyticus and vibrio vulnificus. | the inhibitory effect of igy against vibrio parahaemolyticus and vibrio vulnificus responsible for seafood-borne diseases was investigated in this study. water-soluble fractions (wsf) of protein containing igys were isolated from the egg yolk of hens initially immunized with formalin inactivated v. parahaemolyticus or v. vulnificus. protein, total and specific igy contents of the wsf were determined. the inhibitory and protective effects of igys on the growth of v. parahaemolyticus and v. vulnif ... | 2012 | 23075795 |
| development of o-serogroup specific pcr assay for detection and identification of vibrio parahaemolyticus. | vibrio parahaemolyticus is a human pathogen that is widely disseminated in estuarine, marine and coastal environments throughout the world, and is recognized as the leading cause of food-borne illness worldwide. v. parahaemolyticus infections have been characterized by causal associations with multiple, diverse serotypes. to date, 13 o-serogroups have been recognized in v. parahaemolyticus, although only the o-serogroup genetic determinants (ogds) of serogroups o3 and o4 have been sequenced. in ... | 2012 | 23072697 |
| transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in mactra veneriformis. | the response of the defense components lysozyme (lyz), metallothionein (mt), and superoxide dismutase (sod) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam mactra veneriformis. first, the full-length lyz cdna containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. multiple alignments revealed that mvlyz had a high identity with invertebrate-type lyzs from other mollusks. next, clams were exposed to vib ... | 2013 | 23058986 |
| nontoxigenic vibrio parahaemolyticus strains causing acute gastroenteritis. | we investigated the virulence properties of four vibrio parahaemolyticus strains causing acute gastroenteritis following consumption of indigenous mussels in italy. the isolated strains were cytotoxic and adhesive but, surprisingly, lacked tdh, trh, and type three secretion system 2 (t3ss2) genes. we emphasize that nontoxigenic v. parahaemolyticus can induce acute gastroenteritis, highlighting the need for more investigation of the pathogenicity of this microorganism. | 2012 | 23052317 |
| rise and fall of pandemic vibrio parahaemolyticus serotype o3:k6 in southern chile. | seafood consumption-related diarrhoea increased drastically in chile when the pandemic strain of vibrio parahaemolyticus serotype o3:k6 reached region de los lagos, where most of chile's seafood is produced. outbreaks peaked in 2005 with 3725 clinical cases in this region and gradually decreased to fewer than 10 cases in 2010 and 2011. we show here that the pandemic strain concurrently vanished from mussels; we also report further environmental data. integration of the 2010/2011 data with those ... | 2013 | 23051148 |
| genetic diversity of clinical and environmental vibrio parahaemolyticus strains from the pacific northwest. | since 1997, cases of vibrio parahaemolyticus-related gastroenteritis from the consumption of raw oysters harvested in washington state have been higher than historical levels. these cases have shown little or no correlation with concentrations of potentially pathogenic v. parahaemolyticus (positive for the thermostable direct hemolysin gene, tdh) in oysters, although significant concentrations of tdh(+) v. parahaemolyticus strains were isolated from shellfish-growing areas in the pacific northwe ... | 2012 | 23042162 |
| prevalence and distribution of vibrio parahaemolyticus in finfish from cochin (south india). | finfish samples obtained from four retail outlets in cochin between june 2009 and june 2010 were investigated for the occurrence of vibrio parahaemolyticus. a total of 182 samples were collected and suspect isolates were identified using standard biochemical tests and were further confirmed by a species-specific tlh gene. v. parahaemolyticus was detected in 45.1% of samples, with demersal fish being more affected than pelagic species. the bacterium was isolated more frequently from the skin and ... | 2013 | 23038073 |
| clinical, epidemiological, and spatial characteristics of vibrio parahaemolyticus diarrhea and cholera in the urban slums of kolkata, india. | there is not much information on the differences in clinical, epidemiological and spatial characteristics of diarrhea due to v. cholerae and v. parahaemolyticus from non-coastal areas. we investigated the differences in clinical, epidemiological and spatial characteristics of the two vibrio species in the urban slums of kolkata, india. | 2012 | 23020794 |
| psp toxin release from the cyanobacterium raphidiopsis brookii d9 (nostocales) can be induced by sodium and potassium ions. | paralytic shellfish poisoning (psp) toxins are a group of naturally occurring neurotoxic alkaloids produced among several genera of primarily freshwater cyanobacteria and marine dinoflagellates. although saxitoxin (stx) and analogs are all potent na(+) channel blockers in vertebrate cells, the functional role of these compounds for the toxigenic microorganisms is unknown. based upon the known importance of monovalent cations (such as sodium) in the maintenance of cellular homeostasis and ion cha ... | 2012 | 22983012 |
| prevalence of salmonella and vibrio spp. in seafood products sold in singapore. | foodborne hazards in seafood have only recently received increased attention in singapore since the illness outbreak in 2009 that was associated with consumption of indian rojak (a traditional salad of fruits, vegetables, and seafood). the microbiological quality of seafood must be evaluated for assurance of food safety. the aim of this study was to evaluate the microbiological quality and to determine the prevalence of salmonella and vibrio spp. in seafood sold in singapore. a total of 116 samp ... | 2012 | 22980017 |
| expression of the type vi secretion system 1 component hcp1 is indirectly repressed by opar in vibrio parahaemolyticus. | the type vi secretion system (t6ss) is bacterial protein injection machinery with roles in virulence, symbiosis, interbacterial interaction, antipathogenesis, and environmental stress responses. there are two t6ss loci, t6ss1 and t6ss2, in the two chromosomes of vibrio parahaemolyticus, respectively. this work disclosed that the master quorum sensing (qs) regulator opar repressed the transcription of hcp1 encoding the structural component hcp1 of t6ss1 in v. parahaemolyticus, indicating that qs ... | 2012 | 22924031 |
| predatory bacteria as natural modulators of vibrio parahaemolyticus and vibrio vulnificus in seawater and oysters. | this study shows that naturally occurring vibrio predatory bacteria (vpb) exert a major role in controlling pathogenic vibrios in seawater and shellfish. the growth and persistence of vibrio parahaemolyticus and vibrio vulnificus were assessed in natural seawater and in the eastern oyster, crassostrea virginica. the pathogens examined were v. vulnificus strain vv1003, v. parahaemolyticus o1:kut (kut stands for k untypeable), and v. parahaemolyticus o3:k6 and corresponding o3:k6 mutants deficient ... | 2012 | 22904049 |
| characteristics of potentially pathogenic vibrios from subtropical mozambique compared with isolates from tropical india and boreal sweden. | reported outbreaks of vibrio parahaemolyticus have increased worldwide, particularly in regions of high seafood consumption. in mozambique, seafood constitutes an important food resource and diarrheal diseases are common among its inhabitants. edible clams were collected in maputo bay during both the dry and rainy seasons, with the results showing the number of viable counts of vibrios in clams to peak during the latter. vibrio parahaemolyticus was the predominant species identified among the is ... | 2013 | 22891871 |
| an extensive investigation into the prevalence and the genetic and serological diversity of toxigenic vibrio parahaemolyticus in italian marine coastal waters. | the relationship between vibrio parahaemolyticus strains isolated from the aquatic environment and those isolated from cases of infection in humans is poorly understood due to the low prevalence of tdh- and/or trh-positive strains in the environment. to address this concern, it would be useful to analyse the genetic relationships among environmental and food strains and with reference to clinical isolates, also applying molecular typing methods. the aim of this study was to evaluate the prevalen ... | 2013 | 22891758 |
| [development of a loop-mediated isothermal amplification assay for detecting vibrio parahaemolyticus]. | this study aimed to establish a loop-mediated isothermal amplification (lamp) assay for detection of vibrio parahaemolyticus (v. parahaemolyticus). | 2012 | 22883738 |
| ecology of vibrio parahaemolyticus and vibrio vulnificus in the coastal and estuarine waters of louisiana, maryland, mississippi, and washington (united states). | vibrio parahaemolyticus and vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. sea surface temperature (sst), sea surface height (ssh), and chlorophyll have been shown to be predictors of zooplankton and thus factors linked to vibrio populations. the contribution of salinity, conductivity, turbidity, a ... | 2012 | 22865080 |
| depuration of oysters (crassostrea gigas) contaminated with vibrio parahaemolyticus and vibrio vulnificus with uv light and chlorinated seawater. | the efficacy of depuration using uv light and chlorinated seawater for decontaminating vibrio parahaemolyticus and vibrio vulnificus from oysters was investigated. oysters were contaminated with a five-strain cocktail of v. parahaemolyticus or v. vulnificus to levels of 10(4) to 10(5) cfu ml(-1) for bioaccumulation. the depuration was conducted in a closed system in which 350 liters of seawater was recirculated at a rate of 7 liters/min for 48 h at room temperature. counts of v. parahaemolyticus ... | 2012 | 22856577 |
| inactivation of vibrio parahaemolyticus and vibrio vulnificus in oysters by high-hydrostatic pressure and mild heat. | several recent outbreaks associated with oysters have heightened safety concerns of raw shellfish consumptions, with the majority being attributed to vibrio spp. the objective of this study was to determine the effect of high-hydrostatic pressure (hhp) followed by mild heating on the inactivation of vibrio parahaemolyticus and vibrio vulnificus in live oysters. inoculated oysters were randomly subjected to: a) pressurization at 200-300 mpa for 2 min at 21 °c, b) mild heat treatment at 40, 45 or ... | 2012 | 22850390 |
| vopb1 and vopd1 are essential for translocation of type iii secretion system 1 effectors of vibrio parahaemolyticus. | vibrio parahaemolyticus is a pathogenic vibrio species that causes food-borne acute gastroenteritis, often related to the consumption of raw or undercooked seafood. vibrio parahaemolyticus has 2 type iii secretion systems (t3ss1 and t3ss2). here, we demonstrate that vp1657 (vopb1) and vp1656 (vopd1), which share sequence similarity with pseudomonas genes popb (38%) and popd (36%), respectively, are essential for translocation of t3ss1 effectors into host cells. a vp1680cyaa fusion reporter syste ... | 2012 | 22827847 |
| adaptive acid tolerance response of vibrio parahaemolyticus as affected by acid adaptation conditions, growth phase, and bacterial strains. | vibrio parahaemolyticus strain 690 was isolated from gastroenteritis patients. its thermal and ethanol stress responses have been reported in our previous studies. in this study, we further investigated the effects of various acid adaptation conditions including ph (5.0-6.0) and time (30-90 min) on the acid tolerance in different growth phases of v. parahaemolyticus 690. additionally, the adaptive acid tolerance among different v. parahaemolyticus strains was compared. results indicated that the ... | 2012 | 22827515 |
| bacteriostatic anti-vibrio parahaemolyticus activity of pseudoalteromonas sp. strains dit09, dit44 and dit46 isolated from southern chilean intertidal perumytilus purpuratus. | we characterised the anti-vibrio parahaemolyticus (anti-v. parahaemolyticus) marine bacteria dit09, dit44 and dit46 isolated from the intertidal mussel perumytilus purpuratus. the 16s rrna gene sequences identify a pseudoalteromonas sp. that form a clade with p. prydzensis and p. mariniglutinosa. the strains produced bacteriostatic anti-v. parahaemolyticus agents during the exponential growth phase, which were also active against v. cholerae and v. anguillarum, but not on other gram positive and ... | 2012 | 22806110 |
| a novel tissue inhibitor of metalloproteinase in blood clam tegillarca granosa: molecular cloning, tissue distribution and expression analysis. | tissue inhibitor of metalloproteinases (timps) were originally characterized as inhibitors of matrix metalloproteinases (mmps), but their range of activities has been found to be broader as it includes the inhibition of several of the mmps, etc. the cdna encoding timp-4-like gene from blood clam tegillarca granosa (designated as tg-timp-4-like) which is the first tissue inhibitor of metalloproteinase identified in blood clams, was cloned and characterized. it was of 1164 bp, and an open reading ... | 2012 | 22771965 |
| vp2118 has major roles in vibrio parahaemolyticus response to oxidative stress. | reactive oxygen species (ros), including superoxide anion radical, induce chronic risk of oxidative damage to many cellular macromolecules resulting in damage to cells. superoxide dismutases (sods) catalyze the dismutation of superoxide to oxygen and hydrogen peroxide and are a primary defense against ros. vibrio parahaemolyticus, a marine bacterium that causes acute gastroenteritis following consumption of raw or undercooked seafood, can survive ros generated by intestinal inflammatory cells. h ... | 2012 | 22771831 |
| characterization of vibrio parahaemolyticus genes encoding the systems for utilization of enterobactin as a xenosiderophore. | we determined the ability of vibrio parahaemolyticus to utilize enterobactin (ent) as a xenosiderophore. homology searches of the v. parahaemolyticus genomic sequence revealed the presence of genes that are homologous to the v. cholerae ferric ent utilization genes, which consist of the iron-repressible outer-membrane protein genes irga and vcta, and the atp-binding cassette transport system operon vctpdgc. moreover, the irgb and vctr genes, which encode transcriptional regulators, were also fou ... | 2012 | 22700651 |
| quantitative study of viable vibrio parahaemolyticus cells in raw seafood using propidium monoazide in combination with quantitative pcr. | in this study we developed a specific and sensitive quantitative pcr (qpcr) method combined with a propidium monoazide (pma) sample treatment to quantify tdh-positive viable cells of v. parahaemolyticus in raw seafood (pma-qpcr). the high selectivity of primers and probes were demonstrated by using purified dna from 57 strains belonging to 18 species. using these primers and probes for qpcr and in artificial contamination samples, a good correlation was obtained between ct values and log cfu/rea ... | 2012 | 22677606 |
| complete genome sequence of a novel marine siphovirus, pvp-1, infecting vibrio parahaemolyticus. | among the abundant bacteriophages that belong to the order caudovirales in the ocean, the genome sequences of marine siphoviruses are poorly investigated in comparison to those of myo- or podoviruses. here we report the complete genome sequence of vibrio phage pvp-1, which belongs to the family siphoviridae and infects vibrio parahaemolyticus atcc 33844. | 2012 | 22628398 |
| non-specific immune response and disease resistance induced by siegesbeckia glabrescens against vibrio parahaemolyticus in epinephelus bruneus. | the immunomodulatory effect of siegesbeckia glabrescens extract-supplementation diets on innate immune response and disease resistance of kelp grouper, epinephelus bruneus against vibrio parahaemolyticus at weeks 1, 2, and 4 is reported. the serum lysozyme activity was significantly enhanced with any enriched diet from weeks 1-4 when compared to control diet (0%). the alternative complement haemolytic activities significantly were enhanced with all enriched diets on weeks 2 and 4 whereas the cel ... | 2012 | 22626564 |
| cdna cloning, characterization and expression of lipopolysaccharide and β-1,3-glucan binding protein (lgbp) gene from the indian white shrimp fenneropenaeus indicus. | a 1372bp full length cdna of an lgbp gene was identified from the indian white shrimp, fenneropenaeus indicus. the open reading frame (orf) of the f. indicus lgbp (fein-lgbp) was 1164bp long, encoding a polypeptide of 388 amino acids. the fein-lgbp deduced amino acid has conserved potential recognition motif for β-1, 3 linkages of polysaccharides and putative rgd (arg-gly-asp) cell adhesion sites. phylogenetic analysis revealed that the fein-lgbp was grouped together with lgbps of other crustace ... | 2012 | 22609928 |
| the hydrophilic translocator for vibrio parahaemolyticus, t3ss2, is also translocated. | the pathogenesis of the diarrheal disease caused by vibrio parahaemolyticus, a leading cause of seafood-associated enteritis worldwide, is dependent upon a type iii secretion system, t3ss2. this apparatus enables the pathogen to inject bacterial proteins (effectors) into the cytosol of host cells and thereby modulate host processes. t3ss effector proteins transit into the host cell via a membrane pore (translocon) typically formed by 3 bacterial proteins. we have identified the third translocon ... | 2012 | 22585964 |
| characteristics of a sharp decrease in vibrio parahaemolyticus infections and seafood contamination in japan. | vibrio parahaemolyticus has been one of the most important foodborne pathogens in japan since the 1960s, and a large epidemic was caused by the pandemic serotype o3:k6 from 1997 to 2001. v. parahaemolyticus infections, however, have sharply declined since that time. data on serotypes isolated from 977 outbreaks were collected and analysed. total and pathogenic, thermostable direct hemolysin (tdh) gene-positive v. parahaemolyticus were qualitatively and quantitatively detected in 842 seafood samp ... | 2012 | 22583518 |
| screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the vibrio parahaemolyticus thermolabile hemolysin. | vibrio parahaemolyticus is a halophilic bacterium that is widely distributed in water resources. the bacterium causes lethal food-borne diseases and poses a serious threat to human and animal health all over the world. the major pathogenic factor of v. parahaemolyticus is thermolabile hemolysin (tlh), encoded by the tlh gene, but its toxicity mechanisms are unknown. a high-affinity antibody that can neutralize tlh activity effectively is not available. in this study, we successfully expressed an ... | 2012 | 22562997 |
| [foodborne disease outbreaks around the urban chilean areas from 2005 to 2010]. | foodborne disease outbreaks are one of the main health problems all over the world, which have an extensive impact on human health. | 2012 | 22552507 |
| thermostable direct hemolysin diminishes tyrosine phosphorylation of epidermal growth factor receptor through protein kinase c dependent mechanism. | adequate evidence mounts to the fact that several bacteria and their toxins have protective or curative roles in colorectal cancers. thermostable direct hemolysin (tdh), produced by vibrio parahaemolyticus, down regulates cell proliferation in colon carcinoma cell lines. tdh induces ca2+ influx from an extracellular environment accompanied by protein kinase c phosphorylation. activated protein kinase c inhibits the tyrosine kinase activity of epidermal growth factor receptor (egfr), the rational ... | 2012 | 22543197 |
| biochemical, serological, and virulence characterization of clinical and oyster vibrio parahaemolyticus isolates. | in this study, 77 clinical and 67 oyster vibrio parahaemolyticus isolates from north america were examined for biochemical profiles, serotype, and the presence of potential virulence factors (tdh, trh, and type iii secretion system [t3ss] genes). all isolates were positive for oxidase, indole, and glucose fermentation, consistent with previous reports. the isolates represented 35 different serotypes, 9 of which were shared by clinical and oyster isolates. serotypes associated with pandemic strai ... | 2012 | 22535979 |
| putative type vi secretion systems of vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. | analysis of the genome sequence of vibrio parahaemolyticus reveals two icmf family genes in putative type vi secretion system (vpt6ss) clusters in chromosomes 1 (icmf1) and 2 (icmf2). the icmf1 gene is present in majority of clinical isolates (87.5 %), but has a low fraction (25.0 %) in environmental isolates. however, icmf2 is contained in all strains of both clinical and environmental sources. deletion of either icmf1 or hcp1 significantly reduced bacterial adhesion to caco-2 cells or hela mon ... | 2012 | 22535222 |
| transcriptional regulation of opar, qrr2-4 and apha by the master quorum-sensing regulator opar in vibrio parahaemolyticus. | vibrio parahaemolyticus is a leading cause of infectious diarrhea and enterogastritis via the fecal-oral route. v. harveyi is a pathogen of fishes and invertebrates, and has been used as a model for quorum sensing (qs) studies. luxr is the master qs regulator (mqsr) of v. harveyi, and luxr-dependent expression of its own gene, qrr2-4 and apha have been established in v. harveyi. molecular regulation of target genes by the v. parahaemolyticus mqsr opar is still poorly understood. | 2012 | 22506036 |
| development of a real time pcr assay for rapid detection of vibrio parahaemolyticus from seafood. | a real time pcr assay for the detection of vibrio parahaemolyticus in seafood samples was developed using a novel specific target and a competitive internal amplification control (iac). the specificity of this assay was evaluated using 390 bacterial strains including v. parahaemolyticus, and other strains belonging to vibrio and non-vibrio species. the real time pcr assay unambiguously distinguished v. parahaemolyticus with a detection sensitivity of 4.8 fg per pcr with purified genomic dna or 1 ... | 2012 | 22477699 |
| reductions of vibrio parahaemolyticus in pacific oysters (crassostrea gigas) by depuration at various temperatures. | consumption of raw oysters has been linked to several outbreaks of vibrio parahaemolyticus infection in the united states. this study investigated effects of ice storage and uv-sterilized seawater depuration at various temperatures on reducing v. parahaemolyticus in oysters. raw pacific oysters (crassostrea gigas) were inoculated with a mixed culture of five clinical strains of v. parahaemolyticus (10290, 10292, 10293, be 98-2029 and 027-1c1) at levels of 10⁴⁻⁶ mpn/g. inoculated oysters were eit ... | 2012 | 22475942 |
| probing adenylation: using a fluorescently labelled atp probe to directly label and immunoprecipitate vops substrates. | the bacterial effector vops from vibrio parahaemolyticus modifies host rho gtpases to prevent downstream signalling, which leads to cell rounding and eventually apoptosis. while previous studies have used [α-(32)p] atp for studying this enzyme, we sought to develop a non-radioactive chemical probe of vops function. to guide these studies, the kinetic parameters were determined for a variety of nucleotides and the results indicated that the c6 position of adenosine was amenable to modification. s ... | 2012 | 22456874 |
| identification of differentially expressed genes and quantitative expression of complement genes in the liver of marine medaka oryzias melastigma challenged with vibrio parahaemolyticus. | the innate immune system of fish is the primary defense against acute diseases. the marine medaka oryzias melastigma has been shown to be a potential marine fish model for ecotoxicology, but little is known about the innate immune system of this small fish. in this study, suppression subtractive hybridization (ssh) was used to identify differentially expressed immune genes in the liver of o. melastigma infected with vibrio parahaemolyticus. among the 396 genes identified, based on ncbi blast sea ... | 2012 | 22445008 |
| inflammation and disintegration of intestinal villi in an experimental model for vibrio parahaemolyticus-induced diarrhea. | vibrio parahaemolyticus is a leading cause of seafood-borne gastroenteritis in many parts of the world, but there is limited knowledge of the pathogenesis of v. parahaemolyticus-induced diarrhea. the absence of an oral infection-based small animal model to study v. parahaemolyticus intestinal colonization and disease has constrained analyses of the course of infection and the factors that mediate it. here, we demonstrate that infant rabbits oro-gastrically inoculated with v. parahaemolyticus dev ... | 2012 | 22438811 |