| development and validation of a cultural method for the detection and isolation of salmonella in cloves. | detection of salmonella in some spices, such as cloves, remains a challenge due to their inherent antimicrobial properties. the purpose of this study was to develop an effective detection method for salmonella from spices using cloves as a model. two clove varieties, ceylon and madagascar, were used in the study. cloves were inoculated with salmonella enterica subsp. enterica serotypes montevideo, typhimurium, or weltevreden at about 1, 3, or 6 log cfu/25 g. two test portion sizes, 10 and 25 g, ... | 2017 | 28199150 |
| rapid concentration detection and differentiation of bacteria in skimmed milk using surface enhanced raman scattering mapping on 4-mercaptophenylboronic acid functionalized silver dendrites. | a novel method was developed to rapidly concentrate, detect, and differentiate bacteria in skimmed milk using surface enhanced raman scattering (sers) mapping on 4-mercaptophenylboronic acid (4-mpba) functionalized silver (ag) dendrites. the 4-mpba functionalized ag dendritic sers substrate was used to capture the bacterial cells and enhance the bacterial signal. salmonella, a significantly important food pathogen, was used as the representative strain to optimize and evaluate the developed meth ... | 2017 | 28091716 |
| fowl typhoid in laying hens cause hepatic oxidative stress. | the aim of this study was to analyses nitric oxide, antioxidant status, and oxidative profile in the liver of laying hens naturally infected by salmonella enterica subsp enterica serovar gallinarum (s. gallinarum). the nitrite/nitrate (nox), reactive oxygen species (ros), thiobarbituric acid-reactive substances (tbars), catalase (cat), glutathione reductase (gr), glutathione peroxidase (gpx) and glutathione s-transferase (gst) activities were measured in liver samples, as well the biomarkers of ... | 2017 | 28027943 |
| draft genome sequence of a multidrug-resistant cmy-2-producing salmonella enterica subsp. enterica serovar minnesota st3088 isolated from chicken meat. | | 2017 | 28017672 |
| genome sequences of multidrug-resistant salmonella enterica subsp. enterica serovar infantis strains from broiler chicks in hungary. | three strains of salmonella enterica serovar infantis isolated from healthy broiler chickens from 2012 to 2013 have been sequenced. comparison of these and previously published s infantis genome sequences of broiler origin in 1996 and 2004 will provide new insight into the genome evolution and recent spread of s infantis in poultry. | 2016 | 27979950 |
| is the evolution of salmonella enterica subsp. enterica linked to restriction-modification systems? | salmonella enterica subsp. enterica bacteria are highly diverse foodborne pathogens that are subdivided into more than 1,500 serovars. the diversity is believed to result from mutational evolution, as well as intra- and interspecies recombination that potentially could be influenced by restriction-modification (rm) systems. the aim of this study was to investigate whether rm systems were linked to the evolution of salmonella enterica subsp. enterica. the study included 221 salmonella enterica ge ... | 2017 | 27822532 |
| complete whole-genome sequence of salmonella enterica subsp. enterica serovar java nctc5706. | salmonellae are a significant cause of morbidity and mortality globally. here, we report the first complete genome sequence for salmonella enterica subsp. enterica serovar java strain nctc5706. this strain is of historical significance, having been isolated in the pre-antibiotic era and was deposited into the national collection of type cultures in 1939. | 2016 | 27811100 |
| biofilm formation, phenotypic production of cellulose and gene expression in salmonella enterica decrease under anaerobic conditions. | salmonella enterica subsp. enterica is one of the main food-borne pathogens. this microorganism combines an aerobic life outside the host with an anaerobic life within the host. one of the main concerns related to s. enterica is biofilm formation and cellulose production. in this study, biofilm formation, morphotype, cellulose production and transcription of biofilm and quorum sensing-related genes of 11 s. enterica strains were tested under three different conditions: aerobiosis, microaerobiosi ... | 2016 | 27592071 |
| ultrasound improves chemical reduction of natural contaminant microbiota and salmonella enterica subsp. enterica on strawberries. | new sanitization methods have been evaluated to improve food safety and food quality and to replace chlorine compounds. however, these new methods can lead to physicochemical and sensory changes in fruits and vegetables. the present study evaluated the effects of acetic acid, peracetic acid, and sodium dodecylbenzenesulfonate isolated or combined with 5min of ultrasound treatment (40khz, 500w) on strawberry quality over 9days of storage at 8°c. the strawberry natural contaminant microbiota (mold ... | 2017 | 27743521 |
| complete genome sequences of 17 canadian isolates of salmonella enterica subsp. enterica serovar heidelberg from human, animal, and food sources. | salmonella enterica subsp. enterica serovar heidelberg is a highly clonal serovar frequently associated with foodborne illness. to facilitate subtyping efforts, we report fully assembled genome sequences of 17 canadian s heidelberg isolates including six pairs of epidemiologically related strains. the plasmid sequences of eight isolates contain several drug resistance genes. | 2016 | 27635008 |
| draft genome sequence of salmonella enterica subsp. enterica serovar blockley strain crjjgf_00147 (phylum gammaproteobacteria). | here, we report a 4.72-mbp draft genome sequence of salmonella enterica subsp. enterica serovar blockley strain crjjgf_00147, isolated from chicken rinse in 2009. | 2016 | 27609923 |
| draft genome sequence of salmonella enterica subsp. enterica serovar putten strain crjjgf_00159 (phylum gammaproteobacteria). | here, we report a 4.90 mbp draft genome sequence of salmonella enterica subsp. enterica serovar putten strain crjjgf_00159 isolated from food animal in 2004. | 2016 | 27587814 |
| antimicrobial resistance in zoonotic nontyphoidal salmonella: an alarming trend? | zoonotic bacteria of the genus salmonella have acquired various antimicrobial resistance properties over the years. the corresponding resistance genes are commonly located on plasmids, transposons, gene cassettes, or variants of the salmonella genomic islands sgi1 and sgi2. human infections by nontyphoidal salmonella isolates mainly result from ingestion of contaminated food. the two predominantly found salmonella enterica subsp. enterica serovars in the usa and in europe are s. enteritidis and ... | 2016 | 27506509 |
| characterization and evaluation of a salmonella enterica serotype senftenberg mutant created by deletion of virulence-related genes for use as a live attenuated vaccine. | natural infections of chickens with salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg) are characterized by low-level intestinal invasiveness and insignificant production of antibodies. in this study, we investigated the potential effects of lon and cpxr gene deletions on the invasiveness of s senftenberg into the intestinal epithelium of chickens and its ability to induce an immune response, conferring protection against s senftenberg infection. with the allelic exchange m ... | 2016 | 27489135 |
| complete genome sequence of salmonella enterica subsp. enterica serovar indiana c629, a carbapenem-resistant bacterium isolated from chicken carcass in china. | the carbapenem-resistant salmonella enterica subsp. enterica serovar indiana strain c629 was isolated from a chicken carcass collected from a slaughterhouse in qingdao, china. the complete genome sequence of c629 contains a circular 4,791,723-bp chromosome and a circular 210,106-bp plasmid. genes involved in carbapenem resistance of this bacterium were identified by whole-genome analysis. | 2016 | 27417837 |
| experimental infection of chickens by a flagellated motile strain of salmonella enterica serovar gallinarum biovar gallinarum. | salmonella enterica subsp. enterica serovar gallinarum biovar gallinarum (sg) causes fowl typhoid (ft), a septicaemic disease which can result in high mortality in poultry flocks. the absence of flagella in sg is thought to favour systemic invasion, since bacterial recognition via toll-like receptor (tlr)-5 does not take place during the early stages of ft. in the present study, chicks susceptible to ft were inoculated with a wild type sg (sg) or its flagellated motile derivative (sg fla(+)). in ... | 2016 | 27387725 |
| comparison of microbial communities isolated from feces of asymptomatic salmonella-shedding and non-salmonella shedding dairy cows. | in the united states salmonella enterica subsp. enterica serotypes kentucky and cerro are frequently isolated from asymptomatic dairy cows. however, factors that contribute to colonization of the bovine gut by these two serotypes have not been identified. to investigate associations between salmonella status and bacterial diversity, as well as the diversity of the microbial community in the dairy cow hindgut, the bacterial and archaeal communities of fecal samples from cows on a single dairy far ... | 2016 | 27313565 |
| complete and closed genome sequences of 10 salmonella enterica subsp. enterica serovar anatum isolates from human and bovine sources. | salmonella enterica is an important pathogen transmitted by numerous vectors. genomic comparisons of salmonella strains from disparate hosts have the potential to further our understanding of mechanisms underlying host specificities and virulence. here, we present the closed genome and plasmid sequences of 10 salmonella enterica subsp. enterica serovar anatum isolates from bovine and human sources. | 2016 | 27257192 |
| molecular identification of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum by a duplex pcr assay. | salmonella enterica subsp. enterica serovar gallinarum biovar gallinarum (s gallinarum) and biovar pullorum (s pullorum) are 2 poultry pathogens that cause major economic losses to the poultry industry worldwide. control of both diseases mainly relies on the adoption of biosecurity programs, and success is dependent on accurate and fast detection. based on this concept, we developed a duplex pcr assay, targeting 2 chromosomal sequences, which allowed us to precisely identify and differentiate s ... | 2016 | 27216724 |
| genome sequences of salmonella enterica subsp. enterica serovar lubbock strains isolated from liver abscesses of feedlot cattle. | the genome sequencing of 13 salmonella enterica subsp. enterica serovar lubbock strains isolated from liver abscesses of feedlot cattle is reported here. the availability of these genomes will help to further understand the etiologic role of salmonella strains in liver abscesses of cattle and will serve as references in microbial trace-back studies to improve food safety. | 2016 | 27151794 |
| draft genome sequences of salmonella enterica subsp. enterica serovar berta atcc 8392 and a nalidixic acid-resistant isolate of this strain. | italic! salmonella entericasubspecies italic! entericaserovar berta has been isolated in multiple animal species and has been implicated in human disease. here, we report a 4.7-mbp draft genome sequence of italic! s. entericaserovar berta (atcc strain 8392) and a nalidixic acid-resistant isolate derived from this strain. | 2016 | 27103707 |
| salmonella infection and carriage in reptiles in a zoological collection. | objective to identify important subspecies and serovars of salmonella enterica in a captive reptile population and clinically relevant risk factors for and signs of illness in salmonella-positive reptiles. design retrospective cross-sectional study. animals 11 crocodilians (4 samples), 78 snakes (91 samples), 59 lizards (57 samples), and 34 chelonians (23 samples) at the bronx zoo from 2000 through 2012. procedures data pertaining to various types of biological samples obtained from reptiles wit ... | 2016 | 27074614 |
| draft whole-genome sequences of salmonella enterica subsp. enterica serovars enteritidis, veneziana, and salford, isolated from herbs. | salmonellais a foodborne pathogen found in a wide variety of sources. here, we report draft genome sequences of threesalmonella entericasubsp.entericaserovars found in herbs: enteritidis, veneziana, and salford, with the latter two being extremely rare in california. | 2016 | 27013038 |
| whole genome sequencing provides insights into the genetic determinants of invasiveness in salmonella dublin. | salmonella enterica subsp. enterica serovar dublin (s. dublin) is one of the non-typhoidal salmonella (nts); however, a relatively high proportion of human infections are associated with invasive disease. we applied whole genome sequencing to representative invasive and non-invasive clinical isolates of s. dublin to determine the genomic variations among them and to investigate the underlying genetic determinants associated with invasiveness in s. dublin. although no particular genomic variation ... | 2016 | 26996313 |
| broilers with low serum mannose-binding lectin show increased fecal shedding of salmonella enterica serovar montevideo. | mannose-binding lectin (mbl) is a key molecule in innate immunity. mbl binds to carbohydrates on the surface of pathogens, initiating the complement system via the lectin-dependent pathway or facilitates opsonophagocytosis. in vivo studies using inbred chicken lines differing in mbl serum concentration indicate that chicken mbl affects salmonella resistance; further studies are imperative in conventional broiler chickens. in this study 104 conventional day-old chickens (offspring from a cross be ... | 2016 | 26994208 |
| complete genome and methylome sequences of salmonella enterica subsp. enterica serovar panama (atcc 7378) and salmonella enterica subsp. enterica serovar sloterdijk (atcc 15791). | salmonella enterica spp. are pathogenic bacteria commonly associated with food-borne outbreaks in human and animals. salmonella enterica spp. are characterized into more than 2,500 different serotypes, which makes epidemiological surveillance and outbreak control more difficult. in this report, we announce the first complete genome and methylome sequences from two salmonella type strains associated with food-borne outbreaks, salmonella enterica subsp. enterica serovar panama (atcc 7378) and salm ... | 2016 | 26988049 |
| draft genome sequence of a salmonella enterica subsp. enterica serovar gallinarum bv. gallinarum isolate associated with fowl typhoid outbreaks in brazil. | salmonella enterica subsp. enterica serovar gallinarum bv. gallinarum strains are bird pathogens causing fowl typhoid (ft). isolate br_rs12 was obtained from a poultry flock with ft in 2014. the sequencing of this genome will enable to track the origin of the recent outbreaks in brazil. | 2016 | 26950322 |
| unraveling the effects of static magnetic field stress on cytosolic proteins of salmonella by using a proteomic approach. | the present study investigated the adaptation of salmonella enterica subsp. enterica serovar hadar to static magnetic field (smf) exposure (200 mt, 9 h). the proteomic analysis provides an overview of potentially important cytosolic proteins that salmonella needs to regulate to survive and adapt to magnetic stress. via 2-dimensional electrophoresis and liquid chromatography tandem mass spectrometry, we compared cytosolic proteomes before and after exposure to magnetic field. a total of 35 protei ... | 2016 | 26928316 |
| complete closed genome sequences of salmonella enterica subsp. enterica serotypes anatum, montevideo, typhimurium, and newport, isolated from beef, cattle, and humans. | salmonella enterica spp. are a diverse group of bacteria with a wide range of virulence potential. to facilitate genome comparisons across this virulence spectrum, we present eight complete closed genome sequences of four s. enterica serotypes (anatum, montevideo, typhimurium, and newport), isolated from various cattle samples and from humans. | 2016 | 26847891 |
| complete genome sequence and methylome of salmonella enterica subsp. enterica cerro, a frequent dairy cow serovar. | salmonella enterica subsp. enterica serovar cerro is an infrequent pathogen of humans and other mammals but is frequently isolated from the hindgut of asymptomatic cattle in the united states. to further understand the genomic determinants of s. cerro specificity for the bovine hindgut, the genome of isolate cfsan001588 was fully sequenced and deposited in the genbank database. | 2016 | 26823571 |
| selection and electrophoretic characterization of salmonella enterica subsp. enterica biocide variants resistant to antibiotics. | the proposed research outlines a serious common concern of salmonella resistance to antimicrobials following prolonged exposure to the disinfectants (biocides). these phenotypes of bacteria could potentially result in hard to treat infections. typical for avian sources, biocide sensitive s. enterica subsp. enterica serovars: typhimurium, enteritidis, virchow and zanzibar and their isogenic biocide-tolerant variants were studied in order to investigate bacteriostatic effect of two commercially av ... | 2015 | 26812813 |
| whole-genome sequencing of salmonella enterica subsp. enterica serovar ouakam isolated from ground turkey. | in this report, we announce the first whole-genome sequencing of salmonella enterica subsp. enterica serovar ouakam strain gnt-01, isolated from ground turkey retail meat. the strain has a chromosome of 5,088,451 bp long, with a g+c content of 52.3%, and a plasmid of 109,715 bp. | 2016 | 26798110 |
| complete genome and methylome sequences of two salmonella enterica spp. | salmonella enterica is responsible for major foodborne outbreaks worldwide. it can cause gastroenteritis characterized by diarrhea, vomiting, and fever. salmonella infections raise public health concerns along with consequential economic impacts. in this report, we announce the first complete genome sequences of salmonella enterica subsp. enterica serovar choleraeuis (s. choleraeuis) atcc 10708 and salmonella enterica subsp. enterica serovar pullorum (s. pullorum) atcc 9120, isolated from patien ... | 2016 | 26798102 |
| comparative evaluation of the mutagenicity and genotoxicity of smoke condensate derived from korean cigarettes. | cigarette smoking is associated with carcinogenesis owing to the mutagenic and genotoxic effects of cigarette smoke. the aim of this study was to evaluate the mutagenic and genotoxic effects of korean cigarettes using in vitro assays. | 2015 | 26796893 |
| salmonella enterica typhimurium fljba operon stability: implications regarding the origin of salmonella enterica i 4,[5],12:i:. | salmonella enterica subsp enterica serovar 4,5,12:i:- has been responsible for many recent salmonella outbreaks worldwide. several studies indicate that this serovar originated from s. enterica subsp enterica serovar typhimurium, by the loss of the flagellar phase ii gene (fljb) and adjacent sequences. however, at least two different clones of s. enterica 4,5,12:i:- exist that differs in the molecular events responsible for fljb deletion. the aim of this study was to test the stability of the fl ... | 2015 | 26782556 |
| complete genome and plasmid sequences of three canadian isolates of salmonella enterica subsp. enterica serovar heidelberg from human and food sources. | isolates of salmonella enterica subsp. enterica serovar heidelberg are often associated with poultry products and may cause severe human illness. here, we report the fully assembled genome and plasmid sequences of three s. heidelberg strains with phage types 9, 29, and 41. | 2016 | 26769926 |
| emergence of a clonal lineage of multidrug-resistant esbl-producing salmonella infantis transmitted from broilers and broiler meat to humans in italy between 2011 and 2014. | we report the spread of a clone of multidrug-resistant (mdr), esbl-producing (blactx-m-1) salmonella enterica subsp. enterica serovar infantis, in the italian broiler chicken industry and along the food-chain. this was first detected in italy in 2011 and led to human infection in italy in 2013-2014.a set (n = 49) of extended-spectrum cephalosporin (esc)-resistant (r) isolates of s. infantis (2011-2014) from humans, food-producing animals and meat thereof, were studied along with a selected set o ... | 2015 | 26716443 |
| complete genome sequences of two outbreak strains of salmonella enterica subsp. enterica serovar thompson associated with cilantro. | salmonella enterica subsp. enterica serovar thompson strains rm1984 (cadph-99a2334) and rm1986 (cadph-99a2345) are associated with a 1999 outbreak in contaminated cilantro. we report here the complete genome sequences and annotation of these two s. thompson strains. these genomes are distinct and provide additional data for our understanding of s. enterica. | 2015 | 26586897 |
| complete genome sequence of salmonella enterica subsp. enterica serovar typhi isolate b/sf/13/03/195 associated with a typhoid carrier in pasir mas, kelantan, malaysia. | we report here the complete genome sequence of salmonella enterica subsp. enterica serovar typhi b/sf/13/03/195 obtained from a typhoid carrier, who is a food handler in pasir mas, kelantan. | 2015 | 26564035 |
| genome sequence of salmonella enterica subsp. enterica serovar typhi isolate pm016/13 from untreated well water associated with a typhoid outbreak in pasir mas, kelantan, malaysia. | salmonella enterica subsp. enterica serovar typhi is a human-restricted pathogen that causes typhoid fever. even though it is a human-restricted pathogen, the bacterium is also isolated from environments such as groundwater and pond water. here, we describe the genome sequence of the salmonella enterica subsp. enterica serovar typhi pm016/13 which was isolated from well water during a typhoid outbreak in kelantan, malaysia, in 2013. | 2015 | 26564032 |
| [a case of osteomyelitis with a granulomatous lesion caused by salmonella infantis]. | we report herein on a case of osteomyelitis with a granulomatous lesion caused by salmonella enterica subsp. enterica serovar infantis (salmonella infantis). a 30-year-old japanese man presenting with a 3-week history of left ankle pain was suspected to have either a tumor or osteomyelitis, on the basis of imaging study findings. he underwent a surgical resection of the lesion. histopathological examination revealed a granuloma. however, s. infantis was cultured from the biopsy sample. cefazolin ... | 2015 | 26554224 |
| detection and molecular characterization of salmonella enterica serovar eppendorf circulating in chicken farms in tunisia. | salmonella enterica subsp. enterica serovar eppendorf, with antigenic formula 1,4,12,[27]:d:1,5, is an infrequent serovar. however, 14% (20 of 142) of the isolates recovered during june-july 2012 in chicken farms in tunisia belonged to s. eppendorf. these isolates were analysed for resistance and virulence profiles. none of them were susceptible to all antimicrobials tested, while 70%, 60%, 50%, 50%, 20% and 5% were resistant to sulphonamides (sul1, sul2 and sul3), streptomycin (aada1-like), tri ... | 2016 | 26537844 |
| first draft genome sequence of salmonella enterica serovar gallinarum strain vtccbaa614, isolated from chicken in india. | salmonella enterica subsp. enterica serovar gallinarum biovar gallinarum causes fowl typhoid (ft), which results in huge economic losses to poultry farmers in india. we report the draft genome sequence of salmonella biovar gallinarum strain vtccbaa614, isolated from a chicken in an ft affected broiler flock. | 2015 | 26494667 |
| subtyping of salmonella food isolates suggests the geographic clustering of serotype telaviv. | salmonella is commonly found in a variety of food products and is a major cause of bacterial foodborne illness throughout the world. in this study, we investigated the prevalence and diversity of salmonella in eight different food types: sheep ground meat, cow ground meat, chicken meat, cow offal, traditional sanliurfa cheese, unripened feta cheese, pistachios, and isot (a spice blend of dried red peppers specific to sanliurfa), traditionally and commonly consumed in turkey. among 192 food sampl ... | 2015 | 26489049 |
| draft genome sequence of salmonella enterica subsp. enterica serovar mishmarhaemek isolated from bovine feces. | salmonella enterica subsp. enterica serovar mishmarhaemek is a gram-negative, non-spore-forming, rod-shaped bacterium implicated in human clinical disease. here, we report a 4.8-mbp draft genome sequence of a nalidixic acid-resistant isolate of s. serovar mishmarhaemek. | 2015 | 26472847 |
| development and comparison of a generic multiple-locus variable-number tandem repeat analysis with pulsed-field gel electrophoresis for typing of salmonella enterica subsp. enterica. | salmonella enterica subsp. enterica causes salmonellosis in humans and animals. serovar-specific multiple-locus variable-number tandem repeat analysis (mlva) is widely used for salmonella surveillance; however, isolates have to be serotyped prior to mlva typing and only the most common serovars can be typed. we developed a mlva scheme for high-discriminatory typing of salmonella. | 2015 | 26440858 |
| antimicrobial resistance and molecular investigation of h2s-negative salmonella enterica subsp. enterica serovar choleraesuis isolates in china. | salmonella enterica subsp. enterica serovar choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in asia, and can also cause severe invasive disease in humans. in this study, 21 s. choleraesuis isolates, detected from 21 patients with diarrhea in china between 2010 and 2011, were found to include 19 h2s-negative s. choleraesuis isolates and two h2s-positive isolates. this is the first report of h2s-negative s. choleraesuis isolated from huma ... | 2015 | 26431037 |
| eric-pcr genotyping of field isolates of salmonella enterica subsp. enterica serovar gallinarum biovars gallinarum and pullorum. | salmonella gallinarum (sg) and salmonella pullorum (sp) have been classified as biovars belonging to salmonella enterica subsp. enterica serovar gallinarum. genetic diversity among isolates of the same biovar can be detected by dna fingerprinting techniques which are useful in epidemiological investigations. in this study, we applied the pcr amplification of enterobacterial repetitive intergenic consensus sequences (eric-pcr) to analyse 45 strains of sg and sp, most of which were isolated from d ... | 2015 | 26365161 |
| draft genome sequence of salmonella enterica subsp. enterica serovar kiambu strain crjjgf_00061 (phylum gammaproteobacteria). | we report a 4.58 mbp draft genome sequence of salmonella enterica subsp. enterica serovar kiambu strain crjjgf_00061 isolated from cattle in 2004. | 2016 | 27389263 |
| draft genome sequence of salmonella enterica subsp. enterica serovar lille strain crjjgf_000101 (phylum gammaproteobacteria). | here, we report a 4.98 mbp draft genome sequence of salmonella enterica subsp. enterica serovar lille strain crjjgf_000101, isolated from ground beef in 2007. | 2016 | 27417829 |
| draft genome sequence of salmonella enterica subsp. enterica serovar widemarsh strain crjjgf_00058 (phylum gammaproteobacteria). | here, we report a 4.73 mbp draft genome sequence of salmonella enterica subsp. enterica serovar widemarsh strain crjjgf_00058, isolated from eggs in 2008. | 2016 | 27417830 |
| prevalence and characterization of monophasic salmonella serovar 1,4,[5],12:i:- of food origin in china. | salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- is a monophasic variant of salmonella typhimurium, which has recently been recognized as an emerging cause of infection worldwide. this bacterium has also ranked among the four most frequent serovars causing human salmonellosis in china. however, there are no reports on its contamination in chinese food. serotyping, polymerase chain reaction, antibiotic resistance, virulotyping, and multilocus sequence typing (mlst) assays were used to i ... | 2015 | 26360603 |
| draft genome sequence of salmonella enterica subsp. enterica serovar napoli strain sn310, cause of a multischool outbreak in milan, italy, in 2014. | we report the draft genome sequence of salmonella enterica subsp. enterica serovar napoli strain sn310, isolated from a stool sample of an affected pupil during a multischool outbreak in 2014 in milan, italy. this represents the first reported draft genome sequence of the emerging serovar napoli. | 2015 | 26358605 |
| draft genome sequence and annotation of phyllosphere-persisting salmonella enterica subsp. enterica serovar livingstone strain cky-s4, isolated from an urban lake in regina, canada. | here, we report the first draft genome sequence of salmonella enterica subsp. enterica serovar livingstone. this s. livingstone strain cky-s4 displayed biofilm formation and cellulose production and could persist on lettuce. this genome may help the study of mechanisms by which enteric pathogens colonize food crops. | 2015 | 26272568 |
| first complete genome sequence of a salmonella enterica subsp. enterica serovar derby strain associated with pork in france. | in france, salmonella enterica subsp. enterica serovar derby is one of the most often isolated serovars in pigs. here, we describe the draft genome sequence of a strain isolated from a pig. this strain had the most frequent pulsed-field gel electrophoresis (pfge) and antimicrobial patterns (s, ssu, t) usually observed in pig production in france. those patterns have been also highlighted in human isolates. | 2015 | 26227607 |
| salmonella occurrence and enterobacteriaceae counts in pig feed ingredients and compound feed from feed mills in ireland. | the purpose of this study was to assess the occurrence of non-typhoidal salmonellae and enterobacteriaceae counts in raw ingredients and compound feeds sampled from feed mills manufacturing pig diets. between november 2012 and september 2013, feed ingredients (n=340) and compound pig feed (n=313) samples were collected from five commercial feed mills and one home compounder at various locations throughout ireland. feed ingredients included cereals, vegetable protein sources and by-products of oi ... | 2015 | 26211839 |
| characterization of the cytolethal distending toxin (typhoid toxin) in non-typhoidal salmonella serovars. | for many putative salmonella enterica subsp. enterica virulence genes, functional characterization across serovars has been limited. cytolethal distending toxin b (cdtb) is an incompletely characterized virulence factor that is found not only in salmonella enterica subsp. enterica serovar typhi (salmonella typhi) and dozens of gram negative bacterial pathogens, but also in non-typhoidal salmonella (nts) serovars. | 2015 | 26207144 |
| whole genome sequencing provides an unambiguous link between salmonella dublin outbreak strain and a historical isolate. | salmonella enterica subsp. enterica serovar dublin is an uncommon cause of human salmonellosis; however, a relatively high proportion of cases are associated with invasive disease. the serotype is associated with cattle. a geographically diffuse outbreak of s. dublin involving nine patients occurred in ireland in 2013. the source of infection was not identified. typing of outbreak associated isolates by pulsed-field gel electrophoresis (pfge) was of limited value because pfge has limited discrim ... | 2016 | 26165314 |
| draft genome sequence of salmonella enterica subsp. enterica serovar give, isolated from an imported chili powder product. | we report the genome sequence of salmonella enterica subsp. enterica serovar give (cfsan012622), isolated from imported chili powder in 2014. this genome contains genes previously reported to be specific only to s. enterica serovar enteritidis. this strain shows a unique pulsed-field gel electrophoresis (pfge) pattern clustering with serovar enteritidis (jeg x01.0005). | 2015 | 26139723 |
| complete genome sequence of salmonella enterica subsp. enterica serovar agona 460004 2-1, associated with a multistate outbreak in the united states. | within the last several years, salmonella enterica subsp. enterica serovar agona has been among the 20 most frequently isolated serovars in clinical cases of salmonellosis. in this report, the complete genome sequence of s. agona strain 460004 2-1 isolated from unsweetened puffed-rice cereal during a multistate outbreak in 2008 was sequenced using single-molecule real-time dna sequencing. | 2015 | 26139714 |
| results of salmonella enterica subsp. enterica serotype identification by salmonella check&trace microarray in international external quality assurance systems. | traditionally salmonella enterica subsp. enterica serotypes are identified by slide agglutination with specific antisera for somatic, flagellar and sometimes capsular antigens. an alternative way is genoserotyping using for example a microarray, eg. commercially available test check&trace salmonella. the goal of this study was to evaluate the check&trace salmonella microarray for salmonella enterica subsp. enterica serotype identification, using salmonella strains provided by reference laborator ... | 2015 | 26084071 |
| improvements to a pcr-based serogrouping scheme for salmonella enterica from dairy farm samples. | molecular serotyping through the use of pcr is a simple and useful technique for characterizing isolates of salmonella enterica subsp. enterica belonging to serogroups b, c1, c2, d1, and e1, which are the majority of the isolates associated with human disease outbreaks. however, many of the salmonella strains currently isolated from dairy farms in the northeastern united states are serovar cerro, a group k strain not detected by this assay. primers from a well-known pcr assay for the identificat ... | 2015 | 26038910 |
| [salmonella meningitis in an infant due to a pet turtle]. | in humans, salmonella most often causes self-limiting gastroenteritis, but more severe symptoms such as sepsis and meningitis can also occur and can sometimes have a fatal outcome. even if the meningitis is not fatal, sequelae such as epilepsy, cranial nerve palsies, and hydrocephalus can occur. in the united states, it has been estimated that approximately 6% of the human cases of salmonellosis can be attributed to contact with reptiles or amphibians. the infection may take place by direct cont ... | 2015 | 26014646 |
| salmonella in raw chicken meat from the romanian seaside: frequency of isolation and antibiotic resistance. | the purpose of this study was to investigate the occurrence of salmonella in raw chicken meat samples collected at the romanian seaside and to evaluate the antimicrobial susceptibility of the isolates. in 2012, 317 chicken meat samples from slaughterhouses (n = 289) and retail markets (n = 28) were evaluated. overall, 13.2% (42) of the samples contained salmonella; 12.8% (37) and 17.8% (5) from the chicken carcasses at slaughterhouses and the fresh meat from retail markets, respectively. eight s ... | 2015 | 25951397 |
| two draft genome sequences of a new serovar of salmonella enterica, serovar lubbock. | salmonella enterica is principally a foodborne pathogen that shows considerable serovar diversity. in this report, we present two draft genome sequences of salmonella enterica subsp. enterica serovar lubbock, a novel serovar. | 2015 | 25883279 |
| draft genome sequence of multidrug resistant salmonella enterica serovar weltevreden isolated from seafood. | salmonella enterica subsp. enterica serovar weltevereden is the most frequent serovar isolated from asia. here, we report a draft genome sequence of multidrug resistant salmonella weltevreden 9 isolated from seafood. whole-genome of this isolate and annotation will help enhance the understanding of this pathogenic multidrug-resistant serovar. | 2015 | 25874015 |
| anti-salmonella activity of volatile compounds of vietnam coriander. | essential oil derived from the fresh leaves of polygonum odoratum lour was tested for their effects on a foodborne bacterium salmonella choleraesuis subsp. choleraesuis atcc 35640 using a broth dilution method. this essential oil showed a significant antibacterial activity against s. choleraesuis at the concentration of 200 µg/ml. twenty-five volatile compounds were characterized from this essential oil by gc-ms, and aldehyde compounds were found abundant and accounted for more than three-fourth ... | 2015 | 25870012 |
| genome-wide methylation patterns in salmonella enterica subsp. enterica serovars. | the methylation of dna bases plays an important role in numerous biological processes including development, gene expression, and dna replication. salmonella is an important foodborne pathogen, and methylation in salmonella is implicated in virulence. using single molecule real-time (smrt) dna-sequencing, we sequenced and assembled the complete genomes of eleven salmonella enterica isolates from nine different serovars, and analysed the whole-genome methylation patterns of each genome. we descri ... | 2015 | 25860355 |
| acquisition of extended-spectrum cephalosporin- and colistin-resistant salmonella enterica subsp. enterica serotype newport by pilgrims during hajj. | gatherings like the hajj involving many people who travel from different parts of the world represent a risk for the acquisition and dissemination of infectious diseases. in this study, acquisition of multidrug-resistant (mdr) salmonella spp. in 2013 hajj pilgrims from marseille, france, was investigated. in total, 267 rectal swabs were collected from 129 participants before their departure and after their return from the pilgrimage as well as during the pilgrimage from patients with diarrhoea. ... | 2015 | 25769786 |
| a fatal case of iliopsoas abscess caused by salmonella enterica serovar choleraesuis that heterogeneously formed mucoid colonies. | salmonella enterica subsp. enterica serovar choleraesuis, an organism highly adapted to swine, rarely causes invasive human infection. we describe a fatal case of salmonella ser. choleraesuis infection developing iliopsoas abscess. a part of organisms recovered from the blood formed mucoid colonies, which became reactive to anti-o antigen antisera after either heat treatment or subculture through semisolid agar. | 2015 | 25737074 |
| complete sequences of six inca/c plasmids of multidrug-resistant salmonella enterica subsp. enterica serotype newport. | multidrug-resistant (mdr) salmonella enterica subsp. enterica serotype newport has been a long-standing public health concern in the united states. we present the complete sequences of six inca/c plasmids from animal-derived mdr s. newport ranging from 80.1 to 158.5 kb. they shared a genetic backbone with s. newport inca/c plasmids psn254 and pam04528. | 2015 | 25720681 |
| resistance phenotypes and genotypes of salmonella enterica subsp. enterica isolates from feed, pigs, and carcasses in brazil. | salmonella enterica subsp. enterica plays a role as a foodborne pathogen worldwide. the consumption of contaminated pork has been associated with human salmonellosis and the increase in antimicrobial resistance among salmonella from pigs and pork products is a concern. a total of 225 salmonella isolates from feed mills, the lairage environment, and the intestinal contents of pigs and carcasses were investigated for their antimicrobial susceptibility. a mic for ciprofloxacin was screened by agar ... | 2015 | 25710159 |
| genome sequences of three salmonella enterica subsp. enterica serovar infantis strains from healthy broiler chicks in hungary and in the united kingdom. | the genome sequences of three strains of salmonella enterica subsp. enterica serovar infantis isolated from broiler chickens in 1994 and 2004 in hungary and in the 1980s in the united kingdom are reported here. a sequence comparison should improve our understanding of the evolution of the genome and spread of s. infantis in poultry. | 2015 | 25676749 |
| salmonella surveillance on fresh produce in retail in turkey. | although turkey is one of the major producers of fruits and vegetables in the world, there has been no information available on the prevalence of pathogens in fresh produce. to fill this gap, we collected 503 fresh produce samples including tomato, parsley, iceberg lettuce, green-leaf lettuce and five different fresh pepper varieties (i.e., green, kapya, bell, mazamort and charleston) from 3 major districts within 9 supermarkets and 3 bazaars in ankara, turkey to investigate the presence of salm ... | 2015 | 25643853 |
| increase in resistance to extended-spectrum cephalosporins in salmonella isolated from retail chicken products in japan. | extended-spectrum β-lactamase (esbl)-producing salmonella are one of the most important public health problems in developed countries. esbl-producing salmonella strains have been isolated from humans in asian countries neighboring japan, along with strains harboring the plasmid-mediated extended-spectrum cephalosporin (esc)-resistance gene, ampc (pampc). however, only a few studies have investigated the prevalence of esc-resistant salmonella in chicken products in japan, which are the main vehic ... | 2015 | 25642944 |
| chicken mannose-binding lectin function in relation to antibacterial activity towards salmonella enterica. | mannose-binding lectin (mbl) is a c-type serum lectin of importance in innate immunity. low serum concentrations of mbl have been associated with greater susceptibility to infections. in this study, binding of purified chicken mbl (cmbl) to salmonella enterica subsp. enterica (s. enterica) serotypes b, c1 and d was investigated by flow cytometry, and staphylococcus aureus (s. aureus) was used for comparison. for s. enterica the c1 serotypes were the only group to exhibit binding to cmbl. further ... | 2015 | 25623031 |
| a novel subpopulation of salmonella enterica serovar infantis strains isolated from broiler chicken organs other than the gastrointestinal tract. | salmonella enterica subsp. enterica serovar infantis strains were isolated from broiler chickens from six farms in japan and the pathogenicity associated with the recently reported 280kbp mega plasmid was examined by possession of the plasmid and histopathology of tissues from these chickens. s. infantis strains were isolated from 10 of 24 chickens. phylogenetic, network and bayesian cluster analyses were used to determine whether these strains were in the previously defined clusters 1-5. phylog ... | 2015 | 25542287 |
| rapid detection and specific differentiation of salmonella enterica subsp. enterica enteritidis, typhimurium and its monophasic variant 4,[5],12:i:- by real-time multiplex pcr. | salmonella enterica is one of the most common zoonotic pathogens worldwide causing clinical diseases in human and animal hosts. targeting a reduction of salmonella prevalence in poultry, the eu set up a microbiological criterion that demands the absence of s. enterica subsp. enterica serovars enteritidis and typhimurium including its monophasic variant with seroformula 4,[5],12:i:- in 25 g of poultry neck skin samples and fresh meat according to regulation (eu) no 1086/2011. we developed and in- ... | 2015 | 25462917 |
| [occurrence and characterization of monophasic salmonella enterica subsp. enterica with antigenic formula 1,4, [5], 12: i:-isolated in poland in 2007-2012]. | salmonella is significant etiological agent of bacterial intestinal infections in poland and other european union countries. since the 90's increasing incidence of monophasic salmonella antigenic formula 1,4,[5],12:i:-has been observed, which are divided into two lineages: spanish and european. more common european lineage are characterized by antimicrobial resistance assut and dt193 phagetype. in many european countries, these organisms have become one of the most commonly isolated serovars. th ... | 2014 | 25369653 |
| first fully closed genome sequence of salmonella enterica subsp. enterica serovar cubana associated with a food-borne outbreak. | salmonella enterica subsp. enterica serovar cubana (salmonella serovar cubana) is associated with human and animal disease. here, we used third-generation, single-molecule, real-time dna sequencing to determine the first complete genome sequence of salmonella serovar cubana cfsan002050, which was isolated from fresh alfalfa sprouts during a multistate outbreak in 2012. | 2014 | 25359917 |
| an evaluation of alternative methods for constructing phylogenies from whole genome sequence data: a case study with salmonella. | comparative genomics based on whole genome sequencing (wgs) is increasingly being applied to investigate questions within evolutionary and molecular biology, as well as questions concerning public health (e.g., pathogen outbreaks). given the impact that conclusions derived from such analyses may have, we have evaluated the robustness of clustering individuals based on wgs data to three key factors: (1) next-generation sequencing (ngs) platform (hiseq, miseq, iontorrent, 454, and solid), (2) algo ... | 2014 | 25332847 |
| a comparison of the bax system method to the u.s. food and drug administration's bacteriological analytical manual and international organization for standardization reference methods for the detection of salmonella in a variety of soy ingredients. | the performances of two dupont bax system pcr assays for detecting salmonella on a variety of low-moisture soy ingredients were evaluated against the u. s. food and drug administration's bacteriological analytical manual (fda bam) method or the international organization for standardization (iso) 6579 reference method. these evaluations were conducted as a single laboratory validation at an iso 17025 accredited third-party laboratory. validations were conducted on five soy ingredients: isolated ... | 2014 | 25285497 |
| studies on the antibacterial activity of khaya senegalensis [(desr.) a. juss)] stem bark extract on salmonella enterica subsp. enterica serovar typhi [(ex kauffmann and edwards) le minor and popoff]. | to study the phytochemical screening and antibacterial activity of the stem bark extracts of khaya senegalensis (k. senegalensis) against salmonella enterica subsp. enterica serovar typhi. | 2014 | 25183098 |
| characterization of an unusual salmonella phage type dt7a and report of a foodborne outbreak of salmonellosis. | salmonella enterica subsp. enterica serovar 4,[5],12,i:- is a monophasic variant of salmonella typhimurium and its occurrence has markedly increased in several european countries in the last ten years. in june 2011, an outbreak of salmonella 4,[5],12,i:- was reported among attendees of a wedding reception in the north-east of italy. the source of this outbreak was identified as a cooked pork product served during the wedding reception. all salmonella isolates from humans and the contaminated por ... | 2014 | 25108760 |
| trs-based pcr as a potential tool for inter-serovar discrimination of salmonella enteritidis, s. typhimurium, s. infantis, s. virchow, s. hadar, s. newport and s. anatum. | salmonella enterica subsp. enterica comprises a number of serovars, many of which pose an epidemiological threat to humans and are a worldwide cause of morbidity and mortality. most reported food infection outbreaks involve the serovars salmonella enteritidis and salmonella typhimurium. rapid identification to determine the primary sources of the bacterial contamination is important to the improvement of public health. in recent years, many dna-based techniques have been applied to genotype salm ... | 2014 | 25063578 |
| phylogenetic and population genetic analysis of salmonella enterica subsp. enterica serovar infantis strains isolated in japan using whole genome sequence data. | salmonella enterica subsp. enterica serovar infantis has been reported to be carried asymptomatically in chickens and isolated from some human patients with diarrhea. the aim of this study was to investigate the phylogeny of s. infantis strains isolated in japan from chicken meat, chicken egg shells, environmental samples from a grading and packing center for chicken eggs (gp center), diarrhea patients, and asymptomatic carriers based on whole genome sequence data. the s. infantis strains were i ... | 2014 | 24999237 |
| development of multiplex pcr for rapid identification of four salmonella serovars most commonly isolated in japan. | more than 2,500 serovars of salmonella species have been reported to date. a multiplex-pcr method was developed and evaluated for discriminating the four salmonella enterica subsp enterica serovars, namely, s. enteritidis, s. typhimurium, s. thompson and s. infantis, most commonly isolated in japan. twenty-two serovars of 84 salmonella strains and 7 species of non-salmonella strains were evaluated using primer pairs specific for the detection of salmonella spp. multiplex pcr generated, with 100% ... | 2014 | 24974650 |
| genome analysis and crispr typing of salmonella enterica serovar virchow. | salmonella enterica subsp. enterica serovar virchow has been recognized as a significant health burden in asia, australia and europe. in addition to its global distribution, s. virchow is clinically significant due to the frequency at which it causes invasive infections and its association with outbreaks arising from food-borne transmission. here, we examine the genome of an invasive isolate of s. virchow svq1 (phage type 8) from an outbreak in southeast queensland, australia. in addition to ide ... | 2014 | 24885207 |
| identification and characterization of salmonella enterica subsp. enterica serovar derby isolates carrying a new aada26 gene cassette in a class 1 integron obtained at pig slaughterhouses. | salmonella enterica subsp. enterica (s.) serovar derby is one of the most prevalent serovars in pigs. twenty-seven multiresistant s. derby isolates, obtained at two pig slaughterhouses in southern brazil, were investigated for their molecular relationships, genotypic resistance and presence of class 1 and 2 integrons. all isolates shared the same xbai-macrorestriction pattern and showed a common resistance genotype with resistance to streptomycin/spectinomycin (aada variant), sulphonamides (sul1 ... | 2014 | 24839926 |
| clonal distribution of monophasic salmonella enterica subsp. enterica serotype 4,[5],12:i:- in the czech republic. | abstract salmonella 4,[5],12:i:- has become the third most common serotype in europe, including the czech republic. in this study, phenotypic and genotypic methods for a more detailed description of this serotype were used. analysis of a limited number of isolates revealed that 76% of them belonged to phage type dt193. also, rare phage types dt208 and u311 were identified. in total, 88.6% of the isolates were resistant to at least four antimicrobial agents. in this study, 24 multilocus variable- ... | 2014 | 24823892 |
| genome sequence of salmonella enterica subsp. enterica strain durban. | we report the genome sequence of salmonella enterica subsp. enterica strain durban, isolated from a patient with salmonellosis and typhoid fever. the strain is closely related to s. enterica subsp. enterica strain p125109 but differs in loss of the se20 prophage and acquisition of a prophage similar to elphis. | 2014 | 24812224 |
| intestinal mucus affinity and biological activity of an orally administered antibacterial and anti-inflammatory peptide. | antimicrobial peptides (amp) provide protection from infection by pathogenic microorganisms and restrict bacterial growth at epithelial surfaces to maintain mucosal homeostasis. in addition, they exert a significant anti-inflammatory activity. here we analysed the anatomical distribution and biological activity of an orally administered amp in the context of bacterial infection and host-microbial homeostasis. | 2015 | 24811998 |
| genome sequences of eight salmonella enterica subsp. enterica serovars isolated from a single dairy farm. | here, we report draft genome sequences of 26 isolates of salmonella enterica subsp. enterica, representing eight serotypes, which were isolated from cows in a pennsylvania dairy herd, the farm on which they were reared, and the associated off-site heifer-raising facility over an 8-year sampling period. | 2014 | 24762931 |
| quinolone resistance mechanisms in salmonella enterica serovars hadar, kentucky, virchow, schwarzengrund, and 4,5,12:i:-, isolated from humans in switzerland, and identification of a novel qnrd variant, qnrd2, in s. hadar. | human isolates of salmonella enterica serovars hadar, kentucky, virchow, schwarzengrund, and the monophasic variant of s. typhimurium, salmonella enterica subsp. enterica serovar 4,5,12:i:- were examined for mutations within the quinolone resistance target genes gyra, gyrb, parc, and pare and for plasmid-mediated resistance genes. differences were observed among the serovars. a novel variant of qnrd, qnrd2, was detected in an s. hadar isolate. | 2014 | 24733466 |
| comparative genomic analysis and virulence differences in closely related salmonella enterica serotype heidelberg isolates from humans, retail meats, and animals. | salmonella enterica subsp. enterica serovar heidelberg (s. heidelberg) is one of the top serovars causing human salmonellosis. recently, an antibiotic-resistant strain of this serovar was implicated in a large 2011 multistate outbreak resulting from consumption of contaminated ground turkey that involved 136 confirmed cases, with one death. in this study, we assessed the evolutionary diversity of 44 s. heidelberg isolates using whole-genome sequencing (wgs) generated by the 454 gs flx (roche) pl ... | 2014 | 24732280 |
| genome sequence of the salmonella enterica subsp. enterica serovar namur strain 05-2929, lacking the salmonella atypical fimbrial operon. | this paper announces the genome sequence and annotation of salmonella enterica subsp. enterica serovar namur strain 05-2929. s. namur is a new serovar (39:z4,z23:-) that was isolated from a patient with salmonellosis in 2005 in namur, belgium, and has been identified as lacking the salmonella atypical fimbrial (saf) operon. | 2014 | 24723724 |
| fully assembled genome sequence for salmonella enterica subsp. enterica serovar javiana cfsan001992. | | 2014 | 24699966 |
| draft genome sequence of salmonella enterica subsp. enterica serovar gallinarum biovar pullorum strain fcav198, a brazilian chicken pathogen. | salmonella enterica subsp. enterica serovar gallinarum biovar pullorum is a bird-restricted pathogen which causes pullorum disease. the strain fcav198 was isolated from a pool of chicken ovaries in brazil, and its genome may be helpful for studies involving molecular mechanisms related to pathogenesis and other related applications. | 2014 | 24558231 |
| multiple-locus variable-number tandem repeat analysis of salmonella enterica subsp. enterica serovar dublin. | salmonella serovar dublin causes disease in cattle and leads to considerable production losses. in humans, severe invasive disease and high mortality rates are reported. the presently available typing methods provide insufficient discrimination within salm. dublin for epidemiological investigations. in this study, we developed a multiple-locus variable-number tandem repeat analysis (mlva) scheme for high discriminatory typing of salm. dublin. | 2014 | 24517207 |
| whole-genome sequencing of salmonella enterica subsp. enterica serovar cubana strains isolated from agricultural sources. | we report the draft genomes of salmonella enterica subsp. enterica serovar cubana strain cvm42234, isolated from chick feed in 2012, and s. cubana strain 76814, isolated from swine in 2004. the genome sizes are 4,975,046 and 4,936,251 bp, respectively. | 2014 | 24459266 |