| viability of brucella suis on storage in liquid culture. | | 1947 | 20251193 |
| effects of penicillin on brucella suis in vitro and in vivo. | | 1947 | 20268038 |
| streptomycin resistance of brucella suis and eberthella typhosa. | | 1947 | 20272834 |
| concentration of brucella suis from broth culture. | | 1946 | 20274686 |
| studies on the nutritional requirements of brucella suis. | | 1947 | 20284942 |
| brucellosis in the united states: role and significance of wildlife reservoirs. | regulatory programs for brucellosis in domestic livestock have been active in the u.s. for almost 80 years. wildlife reservoirs of brucellosis include bison (bison bison) and elk (cervus elaphus nelsonii) for brucella abortus whereas brucella suis is the predominant species infecting feral swine. the persistence of brucellosis in wildlife reservoirs poses a risk for reintroduction of brucella into domestic livestock. reducing the prevalence of brucellosis in wildlife reservoirs is anticipated to ... | 2010 | 20362627 |
| quantitative analysis of virb8-virb9-virb10 interactions provides a dynamic model of type iv secretion system core complex assembly. | type iv secretion systems are multiprotein complexes that translocate macromolecules across the bacterial cell envelope. the type iv secretion system in brucella species encodes 12 virb proteins that permit this pathogen to translocate effectors into mammalian cells, where they contribute to its survival inside the host. the "core" complex proteins are conserved in all type iv secretion systems, and they are believed to form the channel for substrate translocation. we have investigated the in vi ... | 2010 | 20426418 |
| the feasibility of using antigens prepared with rough brucella strains for diagnosis of canine brucellosis. | clinical diagnosis of canine brucellosis is not sensitive enough and a negative blood culture cannot rule out the disease. indirect methods of serological testing such as agar gel immunodiffusion (agid), rapid slide agglutination test (rsat) and indirect enzyme linked immunoassay (ielisa) are preferred for routine diagnosis. since brucella canis shares antigenic components with the brucella ovis and brucella abortus rb51 strain, it would seem that either strain could be used as antigen. we prese ... | 2010 | 20461292 |
| brucella suis infection in domestic pigs and wild boar in croatia. | between the years 2000 and 2004, 93,107 sera from 1,997 pig herds in 11 regions of croatia were tested for the presence of antibodies against brucellosis. positive results were observed in 67 herds from seven regions (mean individual prevalence: approximately 1%; herd prevalence: 3.4%). the herds from all but two of the infected farms were reared outdoors and thus almost certainly came into contact with wildlife. from 2003 to 2004, 424 sera, which were randomly collected from hunted wild boar (s ... | 2009 | 20462164 |
| the new species brucella microti replicates in macrophages and causes death in murine models of infection. | the recent isolation of brucella microti from the common vole, the red fox, and the soil raises the possibility of an eventual reemergence of brucellosis in europe. in this work, the pathogenic potential of this new brucella species in both in vitro and in vivo models of infection was analyzed. | 2010 | 20497040 |
| carbonic anhydrase inhibitors. | carbonic anhydrases (cas, ec 4.2.1.1) are widespread enzymes in all organisms, catalyzing co2 hydration to bicarbonate and protons. their inhibition is exploited clinically for decades for various classes of diuretics and systemically acting antiglaucoma agents. in the last years novel applications of ca inhibitors (cais) emerged, such as topically acting antiglaucoma, anticonvulsants, antiobesity, antipain, and antitumor agents/diagnostic tools. such cais target diverse isozymes of the 13 catal ... | 2010 | 20529676 |
| characterization of novel brucella strains originating from wild native rodent species in north queensland, australia. | we report on the characterization of a group of seven novel brucella strains isolated in 1964 from three native rodent species in north queensland, australia, during a survey of wild animals. the strains were initially reported to be brucella suis biovar 3 on the basis of microbiological test results. our results indicated that the rodent strains had microbiological traits distinct from those of b. suis biovar 3 and all other brucella spp. to reinvestigate these rodent strains, we sequenced the ... | 2010 | 20639360 |
| brucella carbonic anhydrases: new targets for designing anti-infective agents. | the facultative intracellular pathogen and zoonotic agent brucella sp. possesses two carbonic anhydrases (cas, ec 4.2.1.1), termed bsca i and bsca ii (in brucella suis), belonging to the β-class of these metalloproteins. these zinc enzymes, present in many other pathogenic bacteria, have been considered recently as potential antibacterial targets. the catalytic activity of bsca ii is higher than that of bsca i (for the conversion of co(2) to bicarbonate). both enzymes were inhibited by the ... | 2010 | 20819063 |
| carbonic anhydrase inhibition/activation: trip of a scientist around the world in the search of novel chemotypes and drug targets. | carbonic anhydrases (cas, ec 4.2.1.1) are metalloenzymes which catalyze co(2) hydration to bicarbonate and protons. five genetically distinct classes are known, which represent an excellent example of convergent evolution. inhibition of α-cas from vertebrates, including humans, with sulfonamides was exploited clinically for decades for various classes of diuretics and systemically acting antiglaucoma agents, whereas newer inhibitors are used as topically acting antiglaucoma drugs, anticonvulsant ... | 2010 | 20819070 |
| brucellosis in northern australia. | the aim of this study was to define the clinical presentation of brucellosis in northern australia and to assess the long-term impact of brucellosis on individual lives. a retrospective review was conducted to assess 32 patients with brucellosis caused by brucella suis in townsville, australia during 1996-2009. all patients were caucasian males with a mean age of 35 years. the greatest risk factor for brucellosis was feral pig hunting (94%, n = 30). there was one laboratory-acquired case. the mo ... | 2010 | 20889883 |
| immune responses and protection against experimental brucella suis biovar 1 challenge in nonvaccinated or b. abortus strain rb51-vaccinated cattle. | twenty hereford heifers approximately 9 months of age were vaccinated with saline (control) or 2 × 10(10) cfu of the brucella abortus strain rb51 (rb51) vaccine. immunologic responses after inoculation demonstrated significantly greater (p < 0.05) antibody and proliferative responses to rb51 antigens in cattle vaccinated with rb51 than in the controls. pregnant cattle received a conjunctival challenge at approximately 6 months of gestation with 10(7) cfu of b. suis bv. 1 strains isolated from na ... | 2010 | 20943881 |
| genomic island 2 is an unstable genetic element contributing to brucella lipopolysaccharide spontaneous smooth-to-rough dissociation. | brucella is a gram-negative bacterium that causes a worldwide-distributed zoonosis. the genus includes smooth (s) and rough (r) species that differ in the presence or absence, respectively, of the o-polysaccharide of lipopolysaccharide. in s brucellae, the o-polysaccharide is a critical diagnostic antigen and a virulence determinant. however, s brucellae spontaneously dissociate into r forms, a problem in antigen and s vaccine production. spontaneous r mutants of brucella abortus, brucella melit ... | 2010 | 20952568 |
| a selective medium for the isolation of brucella suis. | | 1946 | 20984363 |
| brucella suis in aerated broth culture; preliminary studies on growth assays, inoculum, and growth characteristics in an improved medium. | | 1946 | 20998715 |
| brucella suis in aerated broth culture; aeration studies. | | 1946 | 20998716 |
| brucella suis in aerated broth culture; continuous culture studies. | | 1946 | 20998717 |
| studies on brucellosis of swine; exposure and reexposure experiments with brucella suis. | | 1946 | 21013307 |
| orchitis in swine due to brucella suis. | | 1946 | 21020428 |
| aeration in the cultivation of brucella suis. | | 1946 | 21064678 |
| isolation of brucella suis from boar's semen. | | 1945 | 21065726 |
| immunization of mice with gamma-irradiated brucella neotomae and its recombinant strains induces protection against virulent b. abortus, b. melitensis, and b. suis challenge. | human brucellosis, a zoonotic disease of major public health concern in several developing countries, is primarily caused by brucella abortus, brucella melitensis, and brucella suis. no brucellosis vaccine is available for human use. the aim of this study was to determine if brucella neotomae, a bacterium not known to cause disease in any host, can be used for developing brucellosis vaccines. b. neotomae and its recombinant strains overexpressing superoxide dismutase and a 26 kda periplasmic pro ... | 2010 | 21109033 |
| gene expression changes in spleens of the wildlife reservoir species, eurasian wild boar (sus scrofa), naturally infected with brucella suis biovar 2. | brucella suis is responsible for swine brucellosis worldwide. of the five different b. suis biovars (bv.), bv. 2 appears restricted to europe where it is frequently isolated from wild boar and hares, can infect pigs and can cause human brucellosis. in this study, the differential gene expression profile was characterized in spleens of eurasian wild boar naturally infected with b. suis bv. 2. of the 20,201 genes analyzed in the microarray, 633 and 1,373 were significantly (fold change > 1.8; p < ... | 2010 | 21115167 |
| ribosomal rna sequence analysis of brucella infection misidentified as ochrobactrum anthropi infection. | a brucella isolate was identified from purulent material collected during a hip surgery. two previous blood cultures from the same patient yielded ochrobactrum anthropi. after rrna sequencing, all the isolates were identified as brucella species and subsequently serotyped as brucella suis. misidentification of brucella species remains a problem with bacterial identification systems. | 2011 | 21209167 |
| a new β-carbonic anhydrase from brucella suis, its cloning, characterization, and inhibition with sulfonamides and sulfamates, leading to impaired pathogen growth. | a β-carbonic anhydrase (ca, ec 4.2.1.1) from the bacterial pathogen brucella suis, bsca ii, has been cloned, purified, and characterized kinetically. bsca ii showed high catalytic activity for the hydration of co(2) to bicarbonate, with a k(cat) of 1.1×10(6), and k(cat)/k(m) of 8.9×10(7)m(-1)s(-1). a panel of sulfonamides and sulfamates have been investigated for inhibition of this enzyme. all types of activities, from the low nanomolar to the micromolar, have been detected for these derivatives ... | 2010 | 21251841 |
| b. melitensis rough strain b115 is protective against heterologous brucella spp. infections. | brucellosis is one of the most serious zoonoses all over the world, with b. melitensis, b. abortus and b. suis being the most pathogenic species for humans. vaccination of domesticated livestock still represents the most efficient way to prevent human infection. however, the available brucella vaccines retain an important residual virulence and induce antibodies interfering with surveillance programs. moreover, each vaccine shows different protective effects versus different brucella species and ... | 2011 | 21300102 |
| over-expression of homologous antigens in a leucine auxotroph of brucella abortus strain rb51 protects mice against a virulent b. suis challenge. | infection by members of the gram-negative bacterial genus brucella causes brucellosis in a variety of mammals. brucellosis in swine remains a challenge, as there is no vaccine in the usa approved for use in swine against brucellosis. here, we developed an improved recombinant brucella abortus vaccine strain rb51 that could afford protection against brucella suis infection by over-expressing genes encoding homologous proteins: l7/l12 ribosomal protein, cu/zn superoxide dismutase [sod] and glycosy ... | 2011 | 21376799 |
| anti-virulence strategy against brucella suis: synthesis, biological evaluation and molecular modeling of selective histidinol dehydrogenase inhibitors. | in the facultative intracellular pathogen brucella suis, histidinol dehydrogenase (hdh) activity, catalyzing the last step in histidine biosynthesis, is essential for intramacrophagic replication. the inhibition of this virulence factor by substituted benzylic ketones was a proof of concept that disarming bacteria leads to inhibition of intracellular bacterial growth in macrophage infection. this work describes the design, synthesis and evaluation of 19 new potential hdh inhibitors, using a comb ... | 2011 | 21461427 |
| proinflammatory caspase-2-mediated macrophage cell death induced by a rough attenuated brucella suis strain. | brucella spp. are intracellular bacteria that cause an infectious disease called brucellosis in humans and many domestic and wildlife animals. b. suis primarily infects pigs and is pathogenic to humans. the macrophage-brucella interaction is critical for the establishment of a chronic brucella infection. our studies showed that smooth virulent b. suis strain 1330 (s1330) prevented programmed cell death of infected macrophages and rough attenuated b. suis strain vtrs1 (a vaccine candidate) induce ... | 2011 | 21464087 |
| role of nkg2d and its ligands in the anti-infectious activity of vγ9vδ2 t cells against intracellular bacteria. | human vγ9vδ2 t cells play a crucial role in early immune response to intracellular pathogens. their number is drastically increased in the peripheral blood of patients during the acute phase of brucellosis. in vitro, vγ9vδ2 t cells exhibit strong cytolytic activity against brucella-infected cells and impair intracellular growth of brucella suis in autologous macrophages. vγ9vδ2 t cells use cell contact-dependent mechanisms such as the release of lytic granules and fas-mediated signals to lyse in ... | 2011 | 21469127 |
| a novel isolation method of brucella species and molecular tracking of brucella suis biovar 2 in domestic and wild animals. | brucella suis biovar 2 is the most common aetiological agent of porcine brucellosis in europe. b. suis biovar 2 is considered to have low zoonotic potential, but is a causative agent of reproductive losses in pigs, and it is thus economically important. the multilocus variable-number of tandem repeats genotyping analysis of 16 loci (mlva-16) has proven to be highly discriminatory and is the most suitable assay for simultaneously identifying b. suis and tracking infections. the aim of this study ... | 2011 | 21474256 |
| brucellosis of the european brown hare (lepus europaeus). | the european brown hare (lepus europaeus) is an important reservoir of brucella suis biovar 2 and also of the life-threatening zoonotic agent francisella tularensis. since both bacteria can produce similar gross pathological lesions in this species, laboratory tests are necessary for the final diagnosis. the aim of the present study was to develop an immunohistochemical method for the detection of b. suis infection and to describe the pathological and histological lesions caused by b. suis in eu ... | 2011 | 21511271 |
| the whole genome sequences of four brucella strains. | brucella melitensis and brucella suis are intracellular pathogens to livestock and humans. here we report four genome sequences, the virulent strain b. melitensis m28-12 and vaccine strains b. melitensis m5, m111 and b. suis s2 that show varied virulence and pathogenicity, which will help to design more effective brucellosis vaccine. | 2011 | 21602346 |
| proinflammatory response of human endothelial cells to brucella infection. | although vascular pathologies such as vasculitis, endocarditis and mycotic aneurysms have been described in brucellosis patients, the interaction of brucella with the endothelium has not been characterized. in this study we show that brucella abortus and brucella suis can infect and replicate in primary human umbilical vein endothelial cells (huvec) and in the microvascular endothelial cell line hmec-1. infection led to an increased production of il-8, mcp-1 and il-6 in huvec and hmec-1 cells, a ... | 2011 | 21621633 |
| molecular typing of brucella species isolates from livestock and human. | although host specificity has been observed in different species of brucella, crossing the animal host boundary is likely to occur at any time. in this study, bruce ladder pcr and abortus-melitensis-ovis-suis (amos) pcr assays were used to characterize 47 brucella isolates from indian origin in order to know exact species for understanding epidemiology of brucellosis. out of them, 28, 14, and 5 isolates were found to be brucella abortus, brucella melitensis, and brucella suis, respectively. furt ... | 2011 | 21647774 |
| advanced multiplex pcr assay for differentiation of brucella species. | two new primer sets of a 766- and a 344-bp fragment were introduced into the conventional bruce-ladder pcr assay. this novel multiplex pcr assay rapidly and concisely discriminates brucella canis and brucella microti from brucella suis strains and also may differentiate all of the 10 brucella species. | 2011 | 21666028 |
| new bruce-ladder multiplex pcr assay for the biovar typing of brucella suis and the discrimination of brucella suis and brucella canis. | rapid and specific identification of brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. none of the molecular typing methods described so far are able to discriminate b. suis biovars in a single test and differentiation of b. suis from brucella canis by molecular approaches can be difficult. this article describes a new multiplex pcr assay, suis-ladder, for fast and accurate identification of b. suis at the biovar level and t ... | 2011 | 21782356 |
| a single amino acid change in the transmembrane domain of the virb8 protein affects dimerization, interaction with virb10 and brucella suis virulence. | virb8 is a critical component of the brucella suis type iv secretion system (t4ss). we previously showed that the transmembrane (tm) domain plays an essential role in interactions of this protein with itself and the other proteins of the t4ss. we report that a point mutation in this tm domain stabilizes homodimers of virb8 and heterodimers with virb10. a similar variant of agrobacterium tumefaciens virb8 showed the same phenotype. the b. suis virb8 variant was unable to complement a virb8 mutant ... | 2011 | 21763312 |
| epidemiological and clinical aspects of human brucella suis infection in polynesia. | summaryhigh brucellosis seroprevalence rates in domestic swine herds have been reported in wallis and futuna islands and are associated with a significant burden of human infection by brucella suis, a species that is rarely incriminated in human disease. between 2003 and 2010, seven patients had a positive blood culture for b. suis biovar 1, 11 symptomatic patients had a positive rose bengal test (rbt) and a positive serum agglutination test (sat) and three asymptomatic cases were found to be po ... | 2011 | 21733252 |
| Blood, guts and knife cuts: reducing the risk of swine brucellosis in feral pig hunters in north-west New South Wales, Australia. | Humans who have close contact with livestock, wild or feral animals can risk acquiring zoonotic infections such as brucellosis, Q fever, and leptospirosis. Human infection with <em>Brucella suis</em> (swine brucellosis) usually follows occupational or recreational exposure to infected animals. Worldwide, many cases of human infection follow contact with infected feral pigs. In Australia there is a growing market for the export of 'wild boar' and a considerable number of people are involved in fe ... | 2011 | 22182348 |
| Development and evaluation of a selective medium for Brucella suis. | A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter- ... | 2011 | 21968103 |
| revised genome sequence of brucella suis 1330. | brucella suis is a causative agent of porcine brucellosis. we report the resequencing of the original sample upon which the published sequence of brucella suis 1330 is based and describe the differences between the published assembly and our assembly at 12 loci. | 2011 | 22038969 |
| experimental infection of rabbits (cuniculus orictolagus) with brucella suis biovar 1 isolated from wild hares (lepus europaeus). | brucella suis biovar 1 is the causative agent of brucellosis in several domestic and wild animals and it is a common agent of human brucellosis. european hares (lepus europaeus) have been shown to be infected by b. suis biovar 1 and the transmission to other animals has been suggested. in this work, experimental rabbits (cuniculus orictolagus) were infected with b. suis biovar 1 isolated from wild hares. infected rabbits showed high serological response in 2 weeks after discharge and typical gra ... | 2011 | 22137773 |
| Free-ranging wild boar: a disease threat to domestic pigs in Switzerland? | The risk of transmission of pathogens from free-ranging wild boars (Sus scrofa scrofa) to outdoor domestic pigs (S. scrofa domesticus) is of increasing concern in many European countries. We assess this risk, using Switzerland as an example. We estimated 1) the prevalence of important pathogens in wild boars and 2) the risk of interactions between wild boars and outdoor pigs. First, we tested 252 wild boars from selected areas between 2008 and 2010 for infection with Brucella spp. Bacterial prev ... | 2011 | 22102657 |
| Brucella suis Infection in Dogs, Georgia, USA. | TO THE EDITOR: Brucellosis is a serious, globally distributed zoonotic disease. Humans are susceptible to infection by Brucella suis, B. abortus, B. melitensis, and B. canis and can have lifelong symptoms of undulating fever, enlarged lymph nodes, malaise, and arthritis (1). In 2009, the United States was officially classified free of B. abortus. All states except Texas are classified as stage III (free) for swine brucellosis caused by B. suis (2). | 2011 | 22172146 |
| complete genome sequence of brucella suis vbi22, isolated from bovine milk. | brucella suis is the causative agent of swine brucellosis and is known to be able to infect several different hosts, including cattle, dogs, and horses, without causing disease symptoms. here we report the complete genome sequence of brucella suis vbi22, which was isolated from raw milk from an infected cow. | 2012 | 22275106 |
| identification of the virb operon genes encoding the type iv secretion system, in colombian brucella canis isolates. | canine brucellosis is a zoonotic disease caused by brucella canis. the establishment of intracellular replicative niches of b. canis is mediated by proteins secreted by the type iv secretion system, which is encoded by the virb operon. the characterization of such genes has been conducted in other species of the genus, but not in b. canis. we report the design of a multiplex pcr test for the detection of the virb operon genes of b. canis. primers for each of the 12 genes were designed and evalua ... | 2012 | 23290573 |
| isolation of brucella suis biovar 2 from a wild boar in the abruzzo region of italy. | a female wild boar, aged approximately two years, was found dead by local veterinary services in pianola di roio in l'aquila province situated in the abruzzo region of central italy. the carcass was submitted to the istituto zooprofilattico sperimentale dell'abruzzo e del molise 'g. caporale' in teramo for necropsy. brucella suis biovar 2 was isolated from submandibular lymph nodes. this is the first report of isolation of b. suis in the abruzzo region. several authors agreed in the past on the ... | 2012 | 23277120 |
| bacteraemias in tropical australia: changing trends over a 10-year period. | bacteraemia is an important cause of morbidity and mortality worldwide. this is the largest reported study of bacteraemias in australia. the presence of organisms endemic to the tropical region and the changing trends described have significant implications for empirical antibiotic therapy. this retrospective study examined 8976 blood cultures from townsville hospital, a regional australian hospital located in the tropics over a 10-year period. the rate of bacteraemic episodes during the study p ... | 2012 | 23276769 |
| a bacterial glycoengineered antigen for improved serodiagnosis of porcine brucellosis. | brucellosis is a highly zoonotic disease that affects animals and human beings. brucella suis is the etiological agent of porcine brucellosis and one of the major human brucellosis pathogens. laboratory diagnosis of porcine brucellosis mainly relies on serological tests, and it has been widely demonstrated that serological assays based on the detection of anti o-polysaccharide antibodies are the most sensitive tests. here, we validate a recombinant glycoprotein antigen, an n-formylperosamine o-p ... | 2016 | 26984975 |
| improved variation calling via an iterative backbone remapping and local assembly method for bacterial genomes. | sequencing data analysis remains limiting and problematic, especially for low complexity repeat sequences and transposon elements due to inherent sequencing errors and short sequence read lengths. we have developed a program, reviseq, which uses a hybrid method composed of iterative remapping and local assembly upon a bacterial sequence backbone. application of this method to six brucella suis field isolates compared to the newly revised b. suis 1330 reference genome identified on average 13, 15 ... | 2012 | 22967795 |
| a rare case of seronegative culture--proven infection with brucella suis. | brucellosis is a chronic infection produced by members of the brucella family. diagnosis of this condition requires either isolation of the organism in culture or positive serological tests.we describe a 27-year-old male admitted as a case of pyrexia of unknown origin (puo), who tested negative for brucella igm elisa test on preliminary evaluation but was subsequently diagnosed on the strength of positive blood and bone marrow cultures to be a case of brucellosis secondary to brucella suis infec ... | 2012 | 22905059 |
| fatal case of brucellosis misdiagnosed in early stages of brucella suis infection in a 46-year-old patient with marfan syndrome. | we report a fatal case of brucella suis endocarditis initially misdiagnosed by automated identification systems as ochrobactrum anthropi infection in a patient with a history of marfan syndrome and recreational feral swine hunting. this report emphasizes the need to consider brucellosis as a part of the differential diagnosis of acute febrile illness, particularly in patients with known risk of exposure. | 2012 | 22495564 |
| unexpected brucella suis biovar 2 infection in a dairy cow, belgium. | | 2013 | 24274041 |
| the btaf trimeric autotransporter of brucella suis is involved in attachment to various surfaces, resistance to serum and virulence. | the adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. several evidences have recently shown that this rule is also truth for the intracellular pathogen brucella. brucella suis displays the unipolar bmac and btae adhesins, which belong to the monomeric and trimeric autotransporter (ta) families, respectively. it was previously shown that these adhesins are involved in bacterial adhesion to host cells and ... | 2013 | 24236157 |
| mlva genotyping of brucella melitensis and brucella abortus isolates from different animal species and humans and identification of brucella suis vaccine strain s2 from cattle in china. | in china, brucellosis is an endemic disease and the main sources of brucellosis in animals and humans are infected sheep, cattle and swine. brucella melitensis (biovars 1 and 3) is the predominant species, associated with sporadic cases and outbreak in humans. isolates of b. abortus, primarily biovars 1 and 3, and b. suis biovars 1 and 3 are also associated with sporadic human brucellosis. in this study, the genetic profiles of b. melitensis and b. abortus isolates from humans and animals were a ... | 2013 | 24124546 |
| quantitative analysis of the brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation. | during the infection process, bacteria are confronted with various stress factors including nutrient starvation. in an in vitro model, adaptation strategies of nutrient-starved brucellae, which are facultative intracellular pathogens capable of long-term persistence, were determined. | 2013 | 24007556 |
| entry and elimination of marine mammal brucella spp. by hooded seal (cystophora cristata) alveolar macrophages in vitro. | a high prevalence of brucellapinnipedialis serology and bacteriology positive animals has been found in the northeast atlantic stock of hooded seal (cystophoracristata); however no associated gross pathological changes have been identified. marine mammal brucellae have previously displayed different infection patterns in human and murine macrophages. to investigate if marine mammal brucella spp. are able to invade and multiply in cells originating from a presumed host species, we infected alveol ... | 2013 | 23936159 |
| global rsh-dependent transcription profile of brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses. | in the intracellular pathogen brucella spp., the activation of the stringent response, a global regulatory network providing rapid adaptation to growth-affecting stress conditions such as nutrient deficiency, is essential for replication in the host. a single, bi-functional enzyme rsh catalyzes synthesis and hydrolysis of the alarmone (p)ppgpp, responsible for differential gene expression under stringent conditions. | 2013 | 23834488 |
| btae, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of brucella suis. | brucella is responsible for brucellosis, one of the most common zoonoses worldwide that causes important economic losses in several countries. increasing evidence indicates that adhesion of brucella spp. to host cells is an important step to establish infection. we have previously shown that the bmac unipolar monomeric autotransporter mediates the binding of brucella suis to host cells through cell-associated fibronectin. our genome analysis shows that the b. suis genome encodes several addition ... | 2013 | 23319562 |
| peptide nucleic acids inhibit growth of brucella suis in pure culture and in infected murine macrophages. | peptide nucleic acids (pnas) are single-stranded, synthetic nucleic acid analogues containing a pseudopeptide backbone in place of the phosphodiester sugar-phosphate. when pnas are covalently linked to cell-penetrating peptides (cpps) they readily penetrate the bacterial cell envelope, inhibit expression of targeted genes and cause growth inhibition both of gram-positive and gram-negative bacteria. however, the effectiveness of pnas against brucella, a facultative intracellular bacterial pathoge ... | 2013 | 23305655 |
| thermostable cross-protective subunit vaccine against brucella species. | a subunit vaccine candidate was produced from brucella suis 145 (biovar 4; expressing both the a antigen of brucella abortus and the m antigen of brucella melitensis). the preparation consisted mostly of polysaccharide (ps; >90% [wt/wt]; both cell-associated ps and exo-ps were combined) and a small amount of protein (1 to 3%) with no apparent nucleic acids. vaccinated mice were protected (these had a statistically significant reduction in bacterial colonization compared to that of unvaccinated c ... | 2014 | 25320267 |
| genome sequences of brucella abortus and brucella suis strains isolated from bovine in zimbabwe. | this is a report of whole-genome sequences of a brucella abortus strain and two brucella suis strains isolated from bovine in zimbabwe. these strains were selected based on their origin and data obtained when using multiplex pcr assays, then sequenced using next-generation sequencing technologies. | 2014 | 25342680 |
| multiple-locus variable-number tandem-repeat analysis (mlva) genotyping of human brucella isolates in malaysia. | brucellosis is one of the most common zoonotic diseases worldwide. it can cause acute febrile illness in human and is a major health problem. studies in human brucellosis in malaysia is limited and so far no genotyping studies has been done on brucella isolates. the aim of the study was to determine the genetic diversity among brucella species isolated from human brucellosis, obtained over a 6-year period (2009-2014). | 2015 | 26033227 |
| brucella suis vaccine strain s2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic er-stress. | brucella, which is regarded as an intracellular pathogen responsible for a zoonotic disease called brucellosis, survives and proliferates within several types of phagocytic and non-phagocytic cells. brucella infects not only their preferred hosts but also other domestic and wild animal species, inducing abortion and infertility. therefore, the interaction between uterine cells and brucella is important for understanding the pathogenesis of this disease. in this study, we describe the brucella su ... | 2015 | 25633898 |
| extended multilocus sequence analysis to describe the global population structure of the genus brucella: phylogeography and relationship to biovars. | an extended multilocus sequence analysis (mlsa) scheme applicable to the brucella, an expanding genus that includes zoonotic pathogens that severely impact animal and human health across large parts of the globe, was developed. the scheme, which extends a previously described nine locus scheme by examining sequences at 21 independent genetic loci in order to increase discriminatory power, was applied to a globally and temporally diverse collection of over 500 isolates representing all 12 known b ... | 2016 | 28066370 |
| serological and molecular investigation for brucellosis in swine in selected districts of uganda. | brucellosis is a notifiable zoonotic disease affecting livestock, humans, and wildlife in uganda. pigs can be infected with human pathogenic brucella suis biovars 1 and 3 and can be a significant source of brucellosis for humans. uganda has a rapidly growing pig population, and the pork consumption per capita is the highest in east africa. the objective of this work was to determine the seroprevalence of brucellosis in ugandan pigs. a cross-sectional serosurvey of pigs was conducted in three of ... | 2016 | 27142028 |
| marine mammal brucella reference strains are attenuated in a balb/c mouse model. | brucellosis is a zoonosis of worldwide distribution with numerous animal host species. since the novel isolation of brucella spp. from marine mammals in 1994 the bacteria have been isolated from various marine mammal hosts. the marine mammal reference strains brucella pinnipedialis 12890 (harbour seal, phoca vitulina) and brucella ceti 12891 (harbour porpoise, phocoena phocoena) were included in genus brucella in 2007, however, their pathogenicity in the mouse model is pending. herein this is ev ... | 2016 | 26959235 |
| the complete genome of brucella suis 019 provides insights on cross-species infection. | brucella species are the most important zoonotic pathogens worldwide and cause considerable harm to humans and animals. in this study, we presented the complete genome of b. suis 019 isolated from sheep (ovine) with epididymitis. b. suis 019 has a rough phenotype and can infect sheep, rhesus monkeys and possibly humans. the comparative genome analysis demonstrated that b. suis 019 is closest to the vaccine strain b. suis bv. 1 str. s2. further analysis associated the rsh gene to the pathogenicit ... | 2016 | 26821047 |
| comparative genomic analysis between newly sequenced brucella suis vaccine strain s2 and the virulent brucella suis strain 1330. | brucellosis is a bacterial disease caused by brucella infection. in the late fifties, brucella suis vaccine strain s2 with reduced virulence was obtained by serial transfer of a virulent b. suis biovar 1 strain in china. it has been widely used for vaccination in china since 1971. until now, the mechanisms underlie virulence attenuation of s2 are still unknown. | 2016 | 27645563 |
| novel real-time pcr detection assay for brucella suis. | brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in europe. both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. available serological or pcr based methods lack sensitivity and specificity. | 2015 | 26392898 |
| transcriptome-wide identification of hfq-associated rnas in brucella suis by deep sequencing. | recent breakthroughs in next-generation sequencing technologies have led to the identification of small noncoding rnas (srnas) as a new important class of regulatory molecules. in prokaryotes, srnas are often bound to the chaperone protein hfq, which allows them to interact with their partner mrna(s). we screened the genome of the zoonotic and human pathogen brucella suis 1330 for the presence of this class of rnas. we designed a coimmunoprecipitation strategy that relies on the use of hfq as a ... | 2016 | 26553849 |
| structural basis for the inhibition of helicobacter pylori α-carbonic anhydrase by sulfonamides. | periplasmic α-carbonic anhydrase of helicobacter pylori (hpαca), an oncogenic bacterium in the human stomach, is essential for its acclimation to low ph. it catalyses the conversion of carbon dioxide to bicarbonate using zn(ii) as the cofactor. in h. pylori, neisseria spp., brucella suis and streptococcus pneumoniae this enzyme is the target for sulfonamide antibacterial agents. we present structural analysis correlated with inhibition data, on the complexes of hpαca with two pharmacological inh ... | 2015 | 26010545 |
| the 2.5 å structure of the enterococcus conjugation protein tram resembles virb8 type iv secretion proteins. | conjugative plasmid transfer is the most important means of spreading antibiotic resistance and virulence genes among bacteria and therefore presents a serious threat to human health. the process requires direct cell-cell contact made possible by a multiprotein complex that spans cellular membranes and serves as a channel for macromolecular secretion. thus far, well studied conjugative type iv secretion systems (t4ss) are of gram-negative (g-) origin. although many medically relevant pathogens ( ... | 2012 | 23188825 |
| brucella suis vaccine strain 2 induces endoplasmic reticulum stress that affects intracellular replication in goat trophoblast cells in vitro. | brucella has been reported to impair placental trophoblasts, a cellular target where brucella efficiently replicates in association with the endoplasmic reticulum (er), and ultimately trigger abortion in pregnant animals. however, the precise effects of brucella on trophoblast cells remain unclear. here, we describe the infection and replication of brucella suis vaccine strain 2 (b.suis.s2) in goat trophoblast cells (gtcs) and the cellular and molecular responses induced in vitro. our studies de ... | 2016 | 26904517 |
| risk factors for contacts between wild boar and outdoor pigs in switzerland and investigations on potential brucella suis spill-over. | due to the parallel increase of the number of free-ranging wild boar and domestic pigs reared outdoor, the risk that they interact has become higher. contacts with wild boar can be the origin of disease outbreaks in pigs, as it has been documented for brucellosis in some european countries. this study aimed at quantifying the occurrence of contacts between wild boar and outdoor domestic pigs in switzerland, and identifying risk factors for these contacts. furthermore, exposed pigs were tested fo ... | 2012 | 22817843 |
| a serological and bacteriological survey of brucellosis in wild boar (sus scrofa) in belgium. | brucellosis is frequently reported among wild boar populations in europe. the aim of the study was to assess the epidemiological situation in belgium, regarding the steady increase of wild boar populations over the last decades. several serological tests were used and compared with culture and is711 polymerase chain reaction (pcr), to determine the most suitable combination of diagnostic tools for conducting a successful prevalence study in wildlife. | 2012 | 22709889 |
| complete genome sequences of two central european brucella suis bv. 2 haplotype 2c strains isolated from wild boars. | the brucella suis haplotype 2c is commonly isolated from wild boars and domestic pigs across central europe, though it is rarely described in the iberian peninsula. we report here the complete and annotated genome sequences of two haplotype 2c strains isolated from wild boars in the northeast region of spain, above the ebro river. | 2014 | 25013144 |
| complete genome sequences of three iberian brucella suis biovar 2 strains isolated from wild boars. | brucella suis biovar 2 is the most common biovar isolated from wild boars (sus scrofa) associated with transmission to outdoor-reared pigs in europe. we report here the complete and annotated genome sequences of three strains isolated from wild boars in portugal and spain and belonging to the iberian clone (haplotypes 2d and 2e). | 2014 | 24994794 |
| emergence of brucella suis in dogs in new south wales, australia: clinical findings and implications for zoonotic transmission. | animal reservoirs of brucellosis constitute an ongoing threat to human health globally, with foodborne, occupational and recreational exposures creating opportunities for transmission. in australia and the united states, hunting of feral pigs has been identified as the principal risk factor for human brucellosis due to brucella suis. following increased reports of canine b. suis infection, we undertook a review of case notification data and veterinary records to address knowledge gaps about tran ... | 2016 | 27613248 |
| pathogen presence in feral pigs and their movement around two commercial piggeries in queensland, australia. | feral pigs are wild animal reservoirs of infectious pathogens transmissible to other species, all of which are transmissible to domestic pigs. the objective of this study was to detect the presence of harmful production-limiting pathogens; brucella suis, leptospira species, lawsonia intracellularis, mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae in a feral pig population within a 10 km radius of two large-scale commercial piggeries in southern queensland, australia. the movement pa ... | 2014 | 24572722 |
| structural analysis and inhibition of trae from the pkm101 type iv secretion system. | gram-negative bacteria use type iv secretion systems (t4sss) for a variety of macromolecular transport processes that include the exchange of genetic material. the pkm101 plasmid encodes a t4ss similar to the well-studied model systems from agrobacterium tumefaciens and brucella suis here, we studied the structure and function of trae, a homolog of virb8 that is an essential component of all t4sss. analysis by x-ray crystallography revealed a structure that is similar to other virb8 homologs but ... | 2016 | 27634044 |
| the brucella suis ibpa heat-shock chaperone is not required for virulence or for expression of the virb type iv secretion system virb8 protein. | brucella suis, facultative intracellular bacterial pathogen of mammals, and agrobacterium tumefaciens, a plant pathogen, both use a virb type iv secretion system (t4ss) to translocate effector molecules into host cells. hspl, an α-crystalline-type small heat-shock protein, acts as a chaperone for the agrobacterium virb8 protein, an essential component of the virb system. an agrobacterium mutant lacking hspl is attenuated due to a misfunctional t4ss. we have investigated whether ibpa (bra0051), t ... | 2014 | 24517122 |
| type iv secretion machinery: molecular architecture and function. | bacteria have evolved several secretion machineries to bring about transport of various virulence factors, nutrients, nucleic acids and cell-surface appendages that are essential for their pathogenesis. t4s (type iv secretion) systems are versatile secretion systems found in various gram-negative and gram-positive bacteria and in few archaea. they are large multisubunit translocons secreting a diverse array of substrates varying in size and nature from monomeric proteins to nucleoprotein complex ... | 2013 | 23356253 |
| highly potent silver-organoalkoxysilane antimicrobial porous nanomembrane. | we used a simple electrospinning technique to fabricate a highly potent silver-organoalkoxysilane antimicrobial composite from agno3-polyvinylpyrrolidone (pvp)/3-aminopropyltrimethoxysilane (aptms)/tetraethoxysilane (teos) solution. spectroscopic and microscopic analyses of the composite showed that the fibers contain an organoalkoxysilane 'skeleton,' 0.18 molecules/nm2 surface amino groups, and highly dispersed and uniformly distributed silver nanoparticles (5 nm in size). incorporation of orga ... | 2013 | 23574791 |
| in this issue - january/february 2017: experimental hendra virus infection of dogs · brucella suis in dogs · cytokines in stored packed red blood cells · transitional lumbosacral vertebrae in labradors · chemotherapy for histiocytic sarcomas · pyothorax and oesophageal foreign bodies · osteochondroma in a horse · detection of bovine ephemeral fever virus. | | 2017 | 28124421 |
| evaluating the risk of pathogen transmission from wild animals to domestic pigs in australia. | wild animals contribute to endemic infection in livestock as well as the introduction, reintroduction and maintenance of pathogens. the source of introduction of endemic diseases to a piggery is often unknown and the extent of wildlife contribution to such local spread is largely unexplored. the aim of the current study was to quantitatively assess the probability of domestic pigs being exposed to different pathogens from wild animals commonly found around commercial piggeries in australia. spec ... | 2016 | 26711303 |
| activation of bovine neutrophils by brucella spp. | brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus brucella. while many species of brucella exist, brucella melitensis, brucella abortus, and brucella suis are the most common pathogens of humans and livestock. the virulence of brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. the aim of this study was to characterize the bo ... | 2016 | 27436438 |
| omics of brucella: species-specific srna-mediated gene ontology regulatory networks identified by computational biology. | brucella is an intracellular bacterium that causes the zoonotic infectious disease, brucellosis. brucella species are currently intensively studied with a view to developing novel global health diagnostics and therapeutics. in this context, small rnas (srnas) are one of the emerging topical areas; they play significant roles in regulating gene expression and cellular processes in bacteria. in the present study, we forecast srnas in three brucella species that infect humans, namely brucella melit ... | 2016 | 27223678 |
| delta-pgm, a new live-attenuated vaccine against brucella suis. | brucellosis is one of the most widespread zoonosis in the world affecting many domestic and wild animals including bovines, goats, pigs and dogs. each species of the brucella genus has a particular tropism toward different mammals being the most relevant for human health brucella abortus, brucella melitensis and brucella suis that infect bovines, goats/camelids and swine respectively. although for b. abortus and b. melitensis there are vaccines available, there is no efficient vaccine to protect ... | 2016 | 26899373 |
| brucella suis strain 2 vaccine is safe and protective against heterologous brucella spp. infections. | brucellosis is a wide spread zoonotic disease that causes abortion and infertility in mammals and leads to debilitating, febrile illness in humans. brucella abortus, brucella melitensis and brucella suis are the major pathogenic species to humans. vaccination with live attenuated b. suis strain 2 (s2) vaccine is an essential and critical component in the control of brucellosis in china. the s2 vaccine is very effective in preventing brucellosis in goats, sheep, cattle and swine. however, there a ... | 2016 | 26626213 |
| clinical and histological features of brucellin skin test responses in brucella suis biovar 2 infected pigs. | current serological tests for swine brucellosis detect antibodies to the brucella o-polysaccharide (o/ps). however, when infections by bacteria carrying cross-reacting o/ps occur, these tests suffer from false positive serological reactions (fpsr), and the skin test with brucella soluble protein extracts is the best diagnostic alternative to differentiate true brucella suis infections from fpsr in pigs. since this test has been seldom used in b. suis infected swine, the clinical and histological ... | 2015 | 25480681 |
| in vitro bactericidal activity of aminoglycosides, including the next-generation drug plazomicin, against brucella spp. | plazomicin is a next-generation aminoglycoside with a potentially unique set of clinical characteristics compared with other aminoglycosides. this study assessed the minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs) of plazomicin against 15 clinical isolates as well as three reference strains representing brucella abortus, brucella melitensis and brucella suis. these data were compared with those obtained for six other aminoglycosides and two aminocyclitols. ... | 2015 | 25459738 |
| advancement of knowledge of brucella over the past 50 years. | fifty years ago, bacteria in the genus brucella were known to cause infertility and reproductive losses. at that time, the genus was considered to contain only 3 species: brucella abortus, brucella melitensis, and brucella suis. since the early 1960s, at least 7 new species have been identified as belonging to the brucella genus (brucella canis, brucella ceti, brucella inopinata, brucella microti, brucella neotomae, brucella ovis, and brucella pinnipedialis) with several additional new species u ... | 2014 | 24981716 |
| a repa-based elisa for discriminating cattle vaccinated with brucella suis 2 from those naturally infected with brucella abortus and brucella melitensis. | the commonest ways of diagnosing brucellosis in animals include the rose-bengal plate agglutination test, the buffered plate agglutination test (bpa), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (i-elisa). however, these methods cannot discriminate the brucella vaccine strain (brucella suis strain 2; b. suis s2) from naturally acquired virulent strains. of the six common brucella species, brucella melitensis, brucella abortus, an ... | 2014 | 24941369 |
| serological survey of brucella canis in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 24830899 |