| effect of lead and ancylostomiasis on phosphatases of heart in swiss albino mice. | administration of 0.01 and 0.1 mg of lead nitrate for 4 and 7 days and infection of ancylostoma caninum larvae orally altered the activation of alkaline phosphatase and acid phosphatase in the hearts of mice when compared to infected animals and controls. alkaline phosphatase activity increased significantly in all drug-treated + infected mice. the level of acid phosphatase decreased significantly in mice exposed to chronic doses of lead. the altered levels of alkaline phosphatase and acid phosp ... | 2002 | 12568465 |
| [studies on immunological reaction of the antiserum of recombinant secreted protein from ancylostoma caninum]. | to analyze the immunological reaction of the antiserum of recombinant secreted protein from ancylostoma caninum with antigens of various species hookworms at different developmental stages. | 2001 | 12571945 |
| characterisation of humoral immune responses in dogs vaccinated with irradiated ancylostoma caninum. | infection with ancylostoma caninum, an intestinal hookworm of dogs, can cause debilitating and potentially life-threatening disease. in the current study, protective immunity to hookworm infection was induced in dogs following vaccination with irradiation-attenuated third-stage larvae (l3) with significant reductions in both worm (p<0.03) and faecal egg counts (p<0.0004) following a challenge infection. vaccination with irradiated l3 and challenge with infective l3 stimulated a dominant antibody ... | 2003 | 12628766 |
| ability of recombinant factor viia to generate thrombin during inhibition of tissue factor in human subjects. | in view of the central role of the tissue factor-factor viia pathway in the initiation of blood coagulation, novel therapeutic strategies aimed at inhibiting this catalytic complex are currently being evaluated. a limitation of this new class of anticoagulants may be the lack of an appropriate strategy to reverse the effect if a bleeding event occurs. the aim of this study was to investigate the in vivo potential of recombinant factor viia (rviia) to induce thrombin generation in healthy subject ... | 2001 | 11382723 |
| the structure and evolution of penelope in the virilis species group of drosophila: an ancient lineage of retroelements. | the penelope element is the key element responsible for mobilization of other transposable elements in the course of hybrid dysgenesis in drosophila virilis. penelope has an unusually complex, highly variable organization in all studied species of the virlis group. thc bridge1 element from the fish fugu rubripes is homologous to penelope, and database searches detected additional homologous sequences among expressed sequence tags from the flatworm schistosoma mansonii and the nematode ancylostom ... | 2001 | 11443348 |
| effect of short-term coyote removal on populations of coyote helminths. | coyote (canis latrans) removal programs often are initiated despite the potential population regulatory mechanism of parasitism with increased coyote density. we investigated the effect of intensive, short-term coyote removal on population levels of helminths in juvenile and adult coyotes from western texas. coyotes were killed by aerial gunning every 3 mo for 2 yr on two 5,000 ha areas, which reduced the overall coyote density of these areas by about 50%. two other 5,000 ha areas were used as c ... | 2002 | 11838229 |
| time-dependent reversal of sepsis-induced pmn uptake and lung vascular injury by expression of cd18 antagonist. | we determined the time-dependent effects of conditional expression of neutrophil inhibitory factor (nif), a specific 41-kda cd18 integrin antagonist, on the time course of nif expression and lung pmn (polymorphonuclear leukocyte) infiltration and vascular injury in a model of escherichia coli-induced sepsis in mice. studies were made in mice transduced with the e-selectin (es) promoter-nif construct (using liposomes) in which the nif cdna was driven by the inflammation- and endothelial cell-spec ... | 2002 | 11880306 |
| in vitro reactivation of ancylostoma caninum tissue-arrested third-stage larvae by transforming growth factor-beta. | developmental arrest in ancylostoma caninum is associated with preparasitic, free-living third-stage (l3) larvae, as well as anthelmintic-resilient hypobiotic l3 larvae within the tissues of an infected dog. with the tissue-arrested larvae, pregnancy and, more specifically, the hormonal effects of estrogen and prolactin mediate reactivation resulting in transmammary transmission of infection to nursing puppies. estrogen and prolactin have been shown to be critically involved in upregulation of t ... | 2001 | 11534634 |
| immune-mediated haemolytic anaemia: possible association with ancylostoma caninum infection in three dogs. | immune-mediated haemolytic anaemia (imha) may be primary or secondary. in primary imha, no underlying cause can be found, whereas secondary imha is triggered by an underlying cause, such as neoplasia, infectious diseases, or drugs. this paper describes 3 dogs with typical signs of imha that was possibly associated with the intestinal parasite ancylostoma caninum. as intestinal helminths can be difficult to diagnose on faecal examination, it would be pertinent to perform multiple faecal examinati ... | 2001 | 11563722 |
| helminths in dogs belonging to people in a resource-limited urban community in gauteng, south africa. | one hundred and sixty-four faecal samples, 148 adhesive tape swabs and 132 blood samples were collected from dogs in boksburg, gauteng, to assess the prevalence of helminth parasites in the area. sixty-nine of these dogs were also necropsied and helminths recovered from the gastro-intestinal tracts. ancylostoma caninum was the most common helminth and was present in 88% of the dogs, followed by dipylidium caninum in 39% of dogs, toxocara canis in 36%, ancylostoma braziliense in 20%, spirocerca l ... | 2001 | 11585088 |
| enzyme-linked immunoelectrotransfer blotting analysis of human serologic responses to infective hookworm larval antigen. | to explore the possibility of using specific antigens for immunodiagnosis of hookworm disease in endemic area. | 1999 | 11593560 |
| influence of parasitic life style on the patterns of codon usage and base frequencies of ancylostoma and necator species. | parametric analyses were used to investigate the nucleotide, codon, and amino acid composition of coding sequences corresponding to hook-worms. ancylostoma caninum and necator americanus. although genomic research has become prevalent within the scientific community, few studies have dealt directly with parasitic species. parasites have existed throughout the history of mankind due to their wide range of distribution in nature and their ability to evade immune detection. an at nucleotide bias wa ... | 2002 | 12214942 |
| improved detection of endoparasite dna in soil sample pcr by the use of anti-inhibitory substances. | although there have been numerous microbial examinations of soil for the presence of human pathogenic developmental parasite stages of ancylostoma caninum and toxocara canis, molecular techniques (e.g. dna extraction, purification and subsequent pcr) have scarcely been applied. here, dna preparations of soil samples artificially contaminated with genomic dna or parasite eggs were examined by pcr. a. caninum and t. canis-specific primers based on the its-2 sequence were used for amplification. af ... | 2002 | 12237140 |
| recombinant nematode anticoagulant protein c2, a novel inhibitor of tissue factor-factor viia activity, abrogates endotoxin-induced coagulation in chimpanzees. | systemic activation of coagulation leading to disseminated intra-vascular coagulation (dic) is an important feature in patients with severe sepsis. tissue factor has been shown to play a primary role in this pathological response, as revealed by the use of specific inhibitors and antagonists of the tissue factor/factor viia pathway. this class of agents has been demonstrated to attenuate the coagulation response in human volunteers with induced low-grade endotoxemia and to reduce mortality in pr ... | 2002 | 12362234 |
| [cloning and expression of specific antigen genes of ancylostoma caninum]. | to search for the gene encoding specific antigen of ancylostoma caninum that induces host's protective immunity. | 2003 | 12884580 |
| characterization of a recombinant immunodiagnostic antigen (nie) from strongyloides stercoralis l3-stage larvae. | due to the process of internal autoinfection, even chronic asymptomatic infections with strongyloides stercoralis have the potential to become severe disseminated disease with fatal outcome. intermittent and scanty larval excretion makes parasitologic diagnosis difficult. serodiagnosis is helpful, but antigen preparation from infective larvae requires access to patients or immunosuppressed experimental animals. for these reasons, attention has turned to recombinant antigens for immunodiagnosis. ... | 2002 | 12467975 |
| [an epidemiological study on zoonoses in korea] | helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ... | 1981 | 12902720 |
| a novel repressor of nif and glna expression in the methanogenic archaeon methanococcus maripaludis. | nitrogen assimilation in the methanogenic archaeon methanococcus maripaludis is regulated by transcriptional repression involving a palindromic 'nitrogen operator' repressor binding sequence. here we report the isolation of the nitrogen repressor, nrpr, from m. maripaludis using dna affinity purification. deletion of the nrpr gene resulted in loss of nitrogen operator binding activity in cell extracts and loss of repression of nif (nitrogen-fixation) and glna (glutamine synthetase) gene expressi ... | 2003 | 12492867 |
| nematode anticoagulant protein c2 reveals a site on factor xa that is important for macromolecular substrate binding to human prothrombinase. | the binding of recombinant nematode anticoagulant protein c2 (napc2) to either factor x or xa is a requisite step in the pathway for the potent inhibition of viia tissue factor. we have used napc2 as a tight binding probe of human xa to investigate protein substrate recognition by the human prothrombinase complex. napc2 binds with high affinity (k(d) approximately 1 nm) to both x and xa in a way that does not require or occlude the active site of the enzyme. in contrast, napc2 is a tight binding ... | 2002 | 12011050 |
| experimental human infection with the dog hookworm, ancylostoma caninum. | to investigate possible routes for human infection by the dog hookworm (ancylostoma caninum). design, setting and participant: relatively small numbers of infective larvae were administered orally and percutaneously to an informed healthy volunteer (j k l) under medical supervision, at intervals between may 1998 and may 1999. | 2003 | 12526725 |
| inhibition of factor viia/tissue factor with nematode anticoagulant protein c2: from unique mechanism to a promising new clinical anticoagulant. | recombinant nematode anticoagulant protein c2 (rnapc2) is a potent (k(i) = 10 pm) inhibitor of the factor viia/tissue factor complex (fviia/tf) that involves the pre-requisite binding to either zymogen or activated factor x (fx) prior to the formation of the final quaternary complex with fviia/tf. the formation of the binary complex with circulating fx governs the pharmacokinetic profile of rnapc2 in humans, resulting in a prolonged elimination half-life of >50 h. the clinical antithrombotic pot ... | 2002 | 12536118 |
| cutaneous larva migrans with parts of the larva in the epidermis. | creeping eruption is usually caused by hookworms, most commonly ancylostoma braziliensis and ancylostoma caninum. because lesions of cutaneous larva migrans have a typical clinical appearance, they are rarely biopsied. specimens usually show spongiotic dermatitis with spongiotic vesicles containing neutrophils and eosinophils and a mixed-cell dermal infiltrate with numerous eosinophils. we report a case with parts of the larva migrans in the epidermis on histologic examination. | 2002 | 12041816 |
| vertical migration by the infective larvae of three species of parasitic nematodes: is the behaviour really a response to gravity? | vertical migration by infective larvae (l3) of 3 species of nematodes was investigated. upright truncated agarose cones were used to test upward migration, and comparable truncated cone-shaped agarose hollows were used to test downward migration. flat agarose plates were control surfaces. when placed at the bases of upright cones, 74% of ancylostoma caninum l3 migrated up, whereas only 16.5% migrated down the indented cones; this latter value was not significantly different from larval migration ... | 2002 | 12553574 |
| ac-far-1, a 20 kda fatty acid- and retinol-binding protein secreted by adult ancylostoma caninum hookworms: gene transcription pattern, ligand binding properties and structural characterisation. | antibody against adult ancylostoma caninum excretory-secretory (es) products was used to immunoscreen a cdna expression library leading to the isolation of cdnas encoding putative hookworm fatty-acid and retinol-binding proteins. ac-far-1 and ac-far-2 cdnas encode open reading frames corresponding to approximately 20kda proteins with 91 percent amino acid identity. ac-far-1 and ac-far-2 exhibit clear similarities to other fars of parasitic nematodes, most closely to two of the far proteins of ca ... | 2003 | 12554085 |
| [effect of roussin's salt ([fe4s3no7]me) on eggs and rhabditic larvae of ancylostoma caninum]. | | 1952 | 12994644 |
| differentiation of the ova of ancylostoma caninum and uncinaria stenocephala in dogs. | | 1953 | 13092396 |
| the effect of certain chemicals on the eggs and larvae of the canine hookworm (ancylostoma caninum). | | 1954 | 13148484 |
| proteolytic activity in extracts of the esophagus of adults of ancylostoma caninum and the effect of immune serum on this activity. | | 1956 | 13295886 |
| the effect of extracts of the amphidial glands, excretory glands, and esophagus of adults of ancylostoma caninum on the coagulation of dog's blood. | | 1956 | 13295887 |
| continuous phenothiazine therapy of dogs artificially infected with ancylostoma caninum. | | 1956 | 13302654 |
| the etiology of visceral larva migrans. ii. comparative larval morphology of ascaris lumbricoides, necator americanus, strongyloides stercoralis and ancylostoma caninum. | | 1956 | 13346428 |
| survival of ancylostoma caninum in vitro. i. | | 1956 | 13415770 |
| evaluation of the role of ancylostoma caninum in humans as a cause of acute and recurrent abdominal pain. | ancylostoma caninum is responsible for cases with eosinophilic enteritis (ee) and unexplained abdominal pain with peripheral eosinophilia in man. ninety-five patients with obscure acute or recurrent abdominal pain and ten asymptomatic healthy parasite free were subjected to thorough history taking, clinical examination, sonography, routine laboratory investigations and serotesting by igg elisa to detect antibodies to excretory/secretory (es) antigens of adult a. caninum and by igg and igg4 weste ... | 1999 | 12561926 |
| the ultrastructure of the intestinal wall of ancylostoma caninum. | | 1959 | 13665457 |
| hookworm cathepsin d aspartic proteases: contributing roles in the host-specific degradation of serum proteins and skin macromolecules. | cathepsin d aspartic proteases of hookworms were recently implicated in the host-specific digestion of haemoglobin by adult parasites. ac-apr-1 from the dog hookworm, ancylostoma caninum and na-apr-1 from the human hookworm, necator americanus, were shown to be expressed in the infective larval stage (l3) as well as adult worms. we now show that both proteases degraded skin macromolecules and serum proteins, some of which were cleaved more readily from permissive definitive hosts as opposed to n ... | 2003 | 12636356 |
| activation of coagulation by administration of recombinant factor viia elicits interleukin 6 (il-6) and il-8 release in healthy human subjects. | the activation of coagulation has been shown to contribute to proinflammatory responses in animal and in vitro experiments. here we report that the activation of coagulation in healthy human subjects by the administration of recombinant factor viia also elicits a small but significant increase in the concentrations of interleukin 6 (il-6) and il-8 in plasma. this increase was absent when the subjects were pretreated with recombinant nematode anticoagulant protein c2, the inhibitor of tissue fact ... | 2003 | 12738659 |
| mating of ancylostoma caninum in relation to blood loss in the host. | | 1964 | 14170767 |
| [the puma (felis concolor) as a new host for ancylostoma caninum (ercolani, 1859) linstow 1889]. | | 1964 | 14174786 |
| [basic studies on parasiticides. 16. insecticidal effects of an azobenzene derivative on ancylostoma caninum larvae in vitro and its parasiticidal effect on ancylostoma caninum]. | | 1964 | 14191985 |
| metabolism of hookworms. ii. glucose metabolism and glycogen synthesis in adult female ancylostoma caninum. | | 1964 | 14201360 |
| molecular cloning of a novel multidomain kunitz-type proteinase inhibitor from the hookworm ancylostoma caninum. | degenerate oligonucleotide primers derived from conserved serine protease inhibitors were used to amplify a 90-base pair (bp) amplicon from an ancylostoma caninum adult-stage complementary deoxyribonucleic acid (cdna) library by polymerase chain reaction (pcr). the amplicon was labeled and used as a probe to screen the library, and a 2,300-bp cdna clone was identified. the 5' end of the molecule was obtained from adult cdna by 5'-race. the complete sequence named a. caninum kunitz-type protease ... | 2003 | 12760667 |
| tissue factor/factor viia inhibitors block angiogenesis and tumor growth through a nonhemostatic mechanism. | an association between cancer and thrombosis has been recognized for more than a century. however, the manner by which tumor growth is regulated by coagulation in vivo remains unclear. to assess the role of coagulation on tumor growth, in vivo, we tested coagulation inhibitors specific for either tissue factor (tf)/factor viia (fviia) complexes or factor xa (fxa) for antitumor activity. here, we show that two inhibitors of tf/fviia, tf pathway inhibitor (tfpi) and the nematode anticoagulant prot ... | 2003 | 12782609 |
| a cathepsin l protease essential for caenorhabditis elegans embryogenesis is functionally conserved in parasitic nematodes. | proteolytic enzymes are involved in processes important to development and survival of many organisms. parasite proteases are considered potential targets of parasite control yet, for most, their precise physiological functions are unknown. validation of potential targets requires analysis of function. we have recently identified a cathepsin l (cpl) cysteine protease, ce-cpl-1, which is essential for embryonic development of the free-living nematode caenorhabditis elegans. we now show that cpl g ... | 2002 | 12076767 |
| evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, gpa2 and gpb5. | the canonical members of the human glycoprotein hormone subunit family of cystine knot-forming polypeptides include the common alpha-subunit, and four beta-subunit genes, fshbeta, lhbeta, tshbeta, and hcgbeta. using pairwise sequence analysis of the complete human genome, we have identified two novel glycoprotein hormone subunit-related genes. based on unique sequence similarity to the alpha- and beta-subunits of glycoprotein hormones, they were named glycoprotein-alpha2 (gpa2) and glycoprotein- ... | 2002 | 12089349 |
| molecular cloning and purification of ac-tmp, a developmentally regulated putative tissue inhibitor of metalloprotease released in relative abundance by adult ancylostoma hookworms. | a cdna encoding a putative tissue inhibitor of metalloprotease was cloned from an ancylostoma caninum adult hookworm cdna library by immunoscreening with anti-hookworm secretory products antiserum. ac-tmp (a. caninum tissue inhibitor of metalloproteinase) is encoded by a 480-bp mrna with a predicted open reading frame of 140 amino acids (molecular weight, 16,100 da) that contains one potential n-linked glycosylation site and an n-terminal cys-x-cys consensus sequence. the open reading frame corr ... | 2002 | 12139214 |
| effect of route of administration of vaccine and challenge on the immunogenic efficiency of double vaccination with irradiated ancylostoma caninum larvae. | | 1965 | 14275207 |
| effect of vaccinations with recombinant fusion proteins on ancylostoma caninum habitat selection in the canine intestine. | laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. the vaccinated dogs were then challenged orally with 400 third-stage infective larvae (l3) of the canine hookworm, ancylostoma caninum. the 3 a. caninum antigens selected were ac-tmp, an adult-specific secreted tissue inhibitor of metalloproteases; ac-ap, an adult-specific secreted factor xa serine protease inhibitor anticoagulant; and ac-arr-1, a catheps ... | 2002 | 12197114 |
| [anatomical studies on the development of ancylostoma caninum]. | | 1961 | 14457904 |
| eosinophil relationship in gut anaphylaxis during experimental ancylostomosis. | changes induced in the circulating and intestinal eosinophil levels and worm load were studied in female swiss albino mice infected with multiple doses of ancylostoma caninum larvae. there was a significant rise of both circulating and intestinal eosinophils in all the infected animals when compared with controls. in all the experimental groups of animals, peak eosinophilia was observed during the third week of post-infection and sustained upto 30th day with slight alterations. these studies ind ... | 2003 | 12860065 |
| [histochemical studies on ancylostoma caninum. (i-ii)]. | | 1961 | 14477283 |
| electroesophagogram of individual hookworm (ancylostoma caninum). | a device for recording regular rhythmic electrical phenomena that are synchronous with esophageal contractions in ancylostoma cantinum is described. | 1962 | 14493038 |
| the second messenger cyclic gmp mediates activation in ancylostoma caninum infective larvae. | the developmentally arrested infective larva (l(3)) of hookworms encounters a host-specific signal during infection that initiates previously suspended developmental pathways. activated l(3) express a parasitic gene set that encodes proteins involved in moulting, growth and development to the adult stage. early events in this activation to parasitism can be investigated using an in vitro larval feeding assay. when ancylostoma caninum l(3) are exposed to a host-like stimulus, they resume feeding ... | 2003 | 12865078 |
| [helminthes infections in the small intestine of stray dogs in ejungbu city, kyunggi do, korea] | one hundred and two stray dogs in ejungbu city of kyunggi-do, korea were examined to reveal out the degree of natural helminthic infection of small intestine. helminthes were collected at autopsy, by scraping the intestinal contents. the collected worms were classified by their morphological characteristics. out of 102 examined, 72 dogs were infected with any helminthes. the common helminthes were dipylidium caninum (47%), ancylostoma caninum (26%), toxascaris leonina (16%) and toxocara canis (1 ... | 1981 | 12902719 |
| ecological analyses of the intestinal helminth communities of the wolf, canis lupus, in spain. | this work describes the ecological characteristics of the intestinal helminth communities of 50 wolves (canis lupus l.) from spain. the species found were classified into three groups according to prevalence, intensity and intestinal distribution. taenia hydatigena pallas, 1766 and uncinaria stenocephala (railliet, 1884) are the core species of the community. taenia multiceps (leske, 1780) is a secondary species. the rest of the species, alaria alata (goeze, 1782), taenia serialis (gervais, 1847 ... | 2003 | 14535350 |
| molecular characterisation of the ancylostoma-secreted protein family from the adult stage of ancylostoma caninum. | the ancylostoma-secreted proteins are a family of nematode-specific cysteine-rich secreted proteins belonging to the pathogenesis-related protein superfamily. previously we reported that third stage infective larvae of ancylostoma caninum produce two different ancylostoma-secreted proteins, a single and double-domain ancylostoma-secreted protein, designated as ancylostoma-secreted protein-1 and ancylostoma-secreted protein-2, respectively. here we report that adult a. caninum hookworms produce a ... | 2003 | 12906874 |
| [the influence of ultraviolet irradiation upon the development and infectivity of hookworm larvae] | the eggs and rhabditoid larvae of canine hookworm were irradiated with ultraviolet rays for one hour at a distance of 10, 20, 30, and 40 cm. the infective stage larvae of the same parasites were irradiated for l, 3, 5 and 14 hours from the same distances. the infective larvae were also exposed under direct sunlight for l, 2, 3 and 4 hours. parasites: ancylostoma caninum was used. eggs were collected in vitro from female adult worms. the worms were kept at 37 degrees c in petri-dish filled with k ... | 1968 | 12913550 |
| [studies on the transmissibility of pathogenic-organisms to liver by migrating larvae of liver fluke and hookworm] | in order to confirm whether the migrating larvae of parasites could carry pathogenic organisms into liver and cause hepatitis, a series of experiments has been carried out. a. clonorchis sinensis: recovery rate of larvae in the abdominal cavity of rabbits: one to seven days after the administration adolescariae were recovered from the abdominal cavity in less than l % of the total number of metacercariae given. generally, 1~6 larvae were found from each animal which was given 900~1,000 metacerca ... | 1968 | 12913551 |
| acquired resistance in mice to the dog hookworm, ancylostoma caninm. | the present study attempted to induce an acquired immunity against the dog hookworm, ancylostoma caninum. the experiments were carried out to demonstrate and confirm whether an acquired resistance could be developed by the repeated lowgrade infections with the infective larvae of a. caninum in the abnormal host of mouse. 1. in order to determined the distribution of hookworm larvae in the lungs and liver of mice after inoculation of infective larvae, 54 mice were inoculated with 1,000 larvae and ... | 1967 | 12913562 |
| [experimental studies on the effects in helminthic infection, with special reference to the gonadectomy of the host] | introduction: concerning the influences of the host's hormone in helminthic infection, solomon(1963) reported that castrated hamsters were less susceptible to nippostrongylus brasiliensis infection than the sham-operated or normal control groups, and addis(1946) demonstrated that testosterone in the male host was necessary for normal growth of hymenolepis diminuta and the egg productive ability was also effected by the hormone. beck(1950, 1952) noted a significant reduction in egg production in ... | 1966 | 12913569 |
| cutaneous larva migrans: the creeping eruption. | cutaneous larva migrans (clm) is the most common tropically acquired dermatosis. it is caused by hookworm larvae, which are in the feces of infected dogs and cats. the condition occurs mainly in the caribbean and new world, and anyone walking barefoot or sitting on a contaminated beach is at risk. ancylostoma braziliense and ancylostoma caninum are the most common hookworms responsible for clm. the lesions, called creeping eruptions, are characteristically erythematous, raised and vesicular, lin ... | 2003 | 12953933 |
| recombinant nematode anticoagulant protein c2 and other inhibitors targeting blood coagulation factor viia/tissue factor. | originally isolated from a haematophagous hookworm, recombinant nematode anticoagulant protein c2 (rnapc2) is an 85-amino acid protein with potent anticoagulant properties. unlike conventional anticoagulants that attenuate blood coagulation via inhibition of thrombin or activated factor x (fxa) at the downstream portion of the cascade, rnapc2 is a potent inhibitor of the activated factor vii/tissue factor complex (fviia/tf), the key physiological initiator of blood coagulation. its mechanism of ... | 2003 | 12974870 |
| the anthelmintic effect of bephenium on ancylostoma caninum. | | 1958 | 13621319 |
| chemical control of larvae of the dog hookworm ancylostoma caninum (ercolani). | | 1951 | 14819522 |
| the effect of ancylostoma caninum infection on erythropoiesis in dogs. | | 1961 | 13754611 |
| infections of ancylostoma caninum in abnormal hosts. | | 1952 | 14928159 |
| investigations of hookworm larvae. i. on the phototropism of infective larvae of ancylostoma caninum. | | 1952 | 14951409 |
| comparative morphology of ancylostoma tubaeforme (zeder, 1800) and ancylostoma caninum (ercolani, 1859). | | 1962 | 14017192 |
| in vitro studies of larvicidal agents against the larvae of ancylostoma caninum. | | 1964 | 14209495 |
| studies on the life history of the hookworms. 8. the morphological studies on the development of the 3rd stage larvae of ancylostoma caninum in the normal host. | | 1964 | 14225788 |
| studies on the life history of the hookworms. ix. the morphological studies on the development of the 4th stage larvae of ancylostoma caninum in the normal host. | | 1964 | 14225792 |
| uk-279,276, a neutrophil inhibitory glycoprotein, in acute stroke: tolerability and pharmacokinetics. | uk-279,276, a recombinant glycoprotein, binds selectively to the cd11b/cd18 integrin on neutrophils and has the potential to modulate the neuroinflammation associated with acute stroke. after preclinical evidence of neuroprotection, uk-279,276 has entered clinical development. the purposes of this study were to evaluate the safety and tolerability of uk-279,276 and to examine its pharmacokinetics and pharmacodynamics (binding to neutrophil cd11b) in patients with acute stroke. | 2003 | 12805489 |
| inhibition of the tissue factor/factor viia pathway does not influence the inflammatory or antibacterial response to abdominal sepsis induced by escherichia coli in mice. | anticoagulants have gained increasing attention for the treatment of sepsis. inhibition of the tissue factor (tf)/factor (f) viia pathway has been shown to attenuate the activation of coagulation and to prevent death in a primate model of sepsis caused by gram-negative bacteria. | 2004 | 15181580 |
| effects of a selective cd11b/cd18 antagonist and recombinant human tissue plasminogen activator treatment alone and in combination in a rat embolic model of stroke. | we evaluated the neuroprotective effect of uk-279,276 (also referred to as recombinant neutrophil inhibitory factor), a selective cd11b/cd18 antagonist, in combination with thrombolytic therapy on focal cerebral ischemia. | 2003 | 12805500 |
| phosphoinositide-3-oh-kinase inhibitor ly294002 prevents activation of ancylostoma caninum and ancylostoma ceylanicum third-stage infective larvae. | the developmentally arrested hookworm infective larva resumes development only after encountering specific host-mediated cues during invasion. these cues activate a signaling pathway that culminates in the resumption of development. in ancylostoma caninum, activation is characterised by the resumption of feeding and the release of excretory/secretory products required for infection. the dauer stage of the free-living nematode caenorhabditis elegans is a developmentally arrested stage analogous t ... | 2004 | 15217729 |
| intestinal obstruction by eosinophilic jejunitis. | eosinophilic enteritis is an uncommon disease that rarely develops as a surgical emergency. although it may be associated with infestation by ancylostoma caninum, its etiology is unknown and often related to a personal or family history of atopy. a transmural involvement may cause intestinal obstruction--more frequently in the jejunum--or even acute abdomen, which may or may not be accompanied by intestinal perforation. the latter two conditions tend to be more commonly associated with ileum dis ... | 2004 | 15259143 |
| ancylostoma ceylanicum anticoagulant peptide-1: role of the predicted reactive site amino acid in mediating inhibition of coagulation factors xa and viia. | hookworm infection is a leading cause of gastrointestinal blood loss and iron deficiency anemia in developing countries. ancylostoma hookworms secrete potent anticoagulants, which have been shown to target coagulation factors xa and the factor viia/tissue factor complex, respectively. the goal of these experiments was to determine the mechanism of action of three recombinant hookworm anticoagulants using in vitro assays. three hookworm coagulation inhibitors were expressed and purified, along wi ... | 2004 | 15279961 |
| e. coli pneumonia induces cd18-independent airway neutrophil migration in the absence of increased lung vascular permeability. | we examined the relationship between neutrophil [polymorphonuclear leukocyte (pmn)] influx and lung vascular injury in response to escherichia coli pneumonia. we assessed lung tissue pmn uptake by measuring myeloperoxidase and transvascular pmn migration by determining pmn counts in lung interstitium and bronchoalveolar lavage fluid (balf) in mice challenged intratracheally with e. coli. lung vascular injury was quantified by determining microvessel filtration coefficient (kf,c), a measure of va ... | 2003 | 12818890 |
| recombinant nematode anticoagulant protein c2, an inhibitor of the tissue factor/factor viia complex, in patients undergoing elective coronary angioplasty. | we investigated the safety and pharmacodynamics of escalating doses of recombinant nematode anticoagulant protein c2 (rnapc2) in patients undergoing elective coronary angioplasty. | 2003 | 12821239 |
| isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult ancylostoma caninum. | hookworms, bloodfeeding intestinal nematodes, are a leading cause of iron deficiency anemia in the developing world. these parasites have evolved potent mechanisms of interfering with mammalian hemostasis, presumably for the purpose of facilitating bloodfeeding. adult ancylostoma caninum worm extracts contain an activity that inhibits platelet aggregation and adhesion by blocking the function of two cell surface integrin receptors, glycoprotein iib/iiia and gpia/iia. using rphplc, the hookworm p ... | 2003 | 12850261 |
| evaluation of the role of the asialoglycoprotein receptor in the clearance of uk-279,276 (recombinant neutrophil inhibitory factor). | 1. uk-279,276 or recombinant neutrophil inhibitory factor (rnif) is a glycoprotein that has been investigated as an intravenous treatment for stroke. previous data indicate that the asialoglycoprotein receptor (asgpr) in the liver might have a role in the uptake and clearance of uk-279,276 from plasma in rats and dogs. 2. biliary elimination of sialo uk-279,276 can be inhibited by the co-administration of asialo fetuin, a known substrate for asgpr. these data are in keeping with sialo uk-279,276 ... | 2003 | 14514443 |
| effect of vaccination with a recombinant fusion protein encoding an astacinlike metalloprotease (mtp-1) secreted by host-stimulated ancylostoma caninum third-stage infective larvae. | laboratory dogs were vaccinated intramuscularly with a recombinant fusion protein (expressed and isolated from escherichia coli) formulated with the glaxo smithkline adjuvant system 02 (as02). the fusion protein encoded ac-mtp-1, a developmentally regulated astacinlike metalloprotease secreted by host-stimulated ancylostoma caninum third-stage larvae (l3). control dogs were injected intramuscularly with an equivalent amount of as02 adjuvant alone. the vaccinated and control dogs were then challe ... | 2003 | 14533704 |
| infective larvae of the human hookworms necator americanus and ancylostoma duodenale differ in their orientation behaviour when crawling on surfaces. | the infective third stage larvae of hookworms infect their hosts by active skin invasion, and they find and recognize their hosts by the behavioural phases of activation, directed crawling, and penetration. here we analyse the orientation of the infective larvae of the human hookworms necator americanus and ancylostoma duodenale when crawling on surfaces. their behaviour differed from that of the larvae of the dog hookworm ancylostoma caninum, but the two species also differed from each other. n ... | 2004 | 15614586 |
| ecological analysis and description of the intestinal helminths present in dogs in mexico city. | the objective of the present study was to determine the prevalence of parasitoses in stray dogs from mexico city, as well as to describe the structure of the intestinal helminth communities present in said hosts. one hundred and twenty dog intestines were collected and dissected to evaluate their parasitic load; finding parasites in 102 dogs (85%). the cestodes collected were: dipylidium caninum in 72 dogs (60%), taenia hydatigena in three (2.5%), taenia pisiformis in two (1.6%), echinococcus gr ... | 2004 | 15631907 |
| haemonchus contortus: molecular characterisation of a small heat shock protein. | a cdna encoding a predicted small heat shock protein, hsp20, was isolated from the parasitic nematode haemonchus contortus. this cdna encoded a predicted protein of 156 amino acids, which had high sequence identity with other nematode small heat shock proteins. southern blot analysis of genomic dna suggested that in h. contortus hsp20 is encoded by a single copy gene. the hsp20 transcript and protein were expressed in the infective larvae (l3), early l4 and adult stages, but expression was not i ... | 2003 | 14552856 |
| [biology and physiopathology of tissue factor and its relevance for cardiovascular diseases]. | tissue factor (tf) is a transmembrane glycoprotein, currently considered as being the major regulator of the coagulation cascade and the initiator of thrombogenesis in vivo. when tf comes in contact with blood, it forms a high-affinity complex with factors vii/viia, activating factors ix and x and thus leading to the formation of an insoluble fibrin clot. the regulation of tf-viia activity plays a key role in blood-vessel wall interactions. selective patterns of cellular expression of tf are obs ... | 2003 | 14558284 |
| pharmacokinetics and anticoagulant properties of the factor viia-tissue factor inhibitor recombinant nematode anticoagulant protein c2 following subcutaneous administration in man. dependence on the stoichiometric binding to circulating factor x. | recombinant nematode anticoagulant protein c2 (rnapc2) is a potent (k(i) =10 pm), inhibitor of the factor viia/tissue factor (fviia/tf) complex that requires the prerequisite binding to zymogen or activated factor x (fx). in two double blind, place-bo-controlled, sequential dose-escalation phase i studies, rnapc2 was found to be safe and well tolerated following single and repeat subcutaneous administrations in healthy human male volunteers at doses ranging from 0.3 to 5 micro g/kg. there was a ... | 2003 | 14597974 |
| virology. new vaccine and treatment excite ebola researchers. | | 2003 | 14615510 |
| inactivation of cd11b in a mouse transgenic model protects against sepsis-induced lung pmn infiltration and vascular injury. | to inactivate chronically the beta2-integrin cd11b (mac-1), we made a transgenic model in mice in which we expressed the cd11b antagonist polypeptide neutrophil inhibitory factor (nif). using these mice, we determined the in vivo effects of cd11b inactivation on polymorphonuclear leukocyte (pmn) function and acute lung injury (ali) induced by escherichia coli septicemia. in wild-type pmns, cd11b expression was induced within 1 h after e. coli challenge, whereas this response was significantly re ... | 2005 | 15831844 |
| treatment of ebola virus infection with a recombinant inhibitor of factor viia/tissue factor: a study in rhesus monkeys. | infection with the ebola virus induces overexpression of the procoagulant tissue factor in primate monocytes and macrophages, suggesting that inhibition of the tissue-factor pathway could ameliorate the effects of ebola haemorrhagic fever. here, we tested the notion that blockade of fviia/tissue factor is beneficial after infection with ebola virus. | 2003 | 14683653 |
| anticoagulant peptides from ancylostoma caninum are immunologically distinct and localize to separate structures within the adult hookworm. | | 2004 | 14698444 |
| rag1 core and v(d)j recombination signal sequences were derived from transib transposons. | the v(d)j recombination reaction in jawed vertebrates is catalyzed by the rag1 and rag2 proteins, which are believed to have emerged approximately 500 million years ago from transposon-encoded proteins. yet no transposase sequence similar to rag1 or rag2 has been found. here we show that the approximately 600-amino acid "core" region of rag1 required for its catalytic activity is significantly similar to the transposase encoded by dna transposons that belong to the transib superfamily. this supe ... | 2005 | 15898832 |
| a serologically diagnosed human case of cutaneous larva migrans caused by ancylostoma caninum. | a 15-year-old boy, who had recently arrived back from a trip to cambodia for a missionary camp, presented with several serpiginous thread-like skin lesions that began as small papules on the left upper extremities 2 weeks before his visit to hospital. the skin lesions were pruritic and erythematous, and had migrated to the chest and abdomen. the histopathological findings showed only lymphocytic and eosinophilic infiltrations in the dermis of the biopsied skin lesion. the patient's serum reacted ... | 2003 | 14699264 |
| recombinant nematode anticoagulant protein c2, an inhibitor of tissue factor/factor viia, attenuates coagulation and the interleukin-10 response in human endotoxemia. | the tissue factor-factor (f)viia complex (tf/fviia) is responsible for the initiation of blood coagulation under both physiological and pathological conditions. recombinant nematode anticoagulant protein c2 (rnapc2) is a potent inhibitor of tf/fviia, mechanistically distinct from tissue factor pathway inhibitor. the first aim of this study was to elucidate the pharmacokinetics and pharmacodynamics of a single intravenous (i.v.) dose of rnapc2. the second aim was to study its effect on endotoxin- ... | 2004 | 14717968 |
| recombinant factor viia as an antidote for anticoagulant treatment. | increasing knowledge on the function of the hemostatic system in vivo and limitations of currently available anticoagulant agents have led to the development of a new generation of anticoagulants. these new agents have a greater specificity towards activated coagulation pathways and factors and are presently being evaluated in clinical studies. the new generation anticoagulants include specific inhibitors of factor iia (melagatran), factor xa (pentasaccharides), and agents that interfere with ti ... | 2004 | 14872424 |
| parasitic helminths of black bear cubs (ursus americanus) from florida. | twenty-two florida black bear (ursus americanus floridanus) cubs (< or = 12 mo old) from florida were examined for endoparasites between 1998 and 2003. eleven species of helminths were found (8 nematodes, 2 trematodes, and 1 acanthocephalan). the most prevalent helminths were ancylostoma caninum (64%), macracanthorhynchus ingens (36%), strongyloides sp. (27%), baylisascaris transfuga (23%), and a. tubaeforme (14%). new host records include a. tubaeforme and brachylaima virginianum. this is the f ... | 2004 | 15040687 |
| prevalence of dogs with intestinal parasites in tochigi, japan in 1979, 1991 and 2002. | a total of 772 canine fecal specimens were collected in tochigi in 1979, 1991 and 2001. the frequency of dogs that were kept indoor in 2002 (72.8%) was significantly higher than that for 1979 (21.2%, p < 0.001) and 1991 (40.6%, p < 0.01). the frequency of parasitized dogs in 2002 (18.8%) was significantly lower than that for 1979 (41.9%, p < 0.01). the findings of this study confirm the declining trend in japan in the prevalence of helminth parasites in dogs, particularly ancylostoma caninum and ... | 2004 | 15041099 |
| cloning and characterisation of genes encoding two transforming growth factor-beta-like ligands from the hookworm, ancylostoma caninum. | to elucidate the role of transforming growth factor beta (tgf-beta) signalling in the arrest/reactivation pathway of the ancylostoma caninum hookworm, two parasite-encoded tgf-beta-like ligands were cloned and characterised. ac-dbl-1 showed 60% amino acid identity to the caenorhabditis elegansdbl-1 gene, which regulates growth while ac-daf-7 showed 46% amino acid identity to ce-daf-7 which regulates arrested development. exon/intron organisation of the genes for ac-dbl-1 and ac-daf-7 were differ ... | 2005 | 16140304 |
| rnapc2. nuvelo. | recombinant nematode anticoagulant protein-2 (rnapc2) is an antithrombotic protein under development by nuvelo as a potential therapeutic agent for the treatment of major acute cardiovascular disease such as unstable angina and myocardial infarction. phase ii clinical trials were ongoing in january 2004, although development for deep vein thrombosis had been suspended by dendreon due to a requirement for a partner to assist with additional phase ii clinical trials. | 2004 | 15083600 |
| biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm ancylostoma caninum. | we report the cloning and expression of ac-gst-1, a novel glutathione s-transferase from the adult hookworm ancylostoma caninum, and its possible role in parasite blood feeding and as a vaccine target. the predicted ac-gst-1 open reading frame contains 207 amino acids (mass, 24 kda) and exhibited up to 65% amino acid identity with other nematode gsts. mrna encoding ac-gst-1 was detected in adults, eggs, and larval stages, but the protein was detected only in adult hookworm somatic extracts and e ... | 2005 | 16177370 |
| vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms. | we expressed a catalytically active cysteine protease, ac-cp-2, from the blood-feeding stage of the canine hookworm ancylostoma caninum and vaccinated dogs with the purified protease. dogs acquired high-titer, antigen-specific antibody responses, and adult hookworms recovered from the intestines of vaccinated dogs were significantly smaller than hookworms from control dogs. there was also a marked decrease in fecal egg counts and the number of female hookworms in vaccinated dogs. ac-cp-2 is expr ... | 2004 | 15122534 |