| humoral response and memory formation in carp after injection of aeromonas hydrophila bacterin. | the humoral immune response of carp (cyprinus carpio) upon a bacterial fish pathogen aeromonas hydrophila was studied in relation to memory formation. after a single intramuscular (i.m.) injection of formalin killed a. hydrophila cells (f-ah), maximum serum ab (ab) titers were observed at day 20. distinct titers were still seen at day 360 in the groups injected with a medium or high antigen (ag) dose (10(7) respectively 10(9) f-ah). the effect of a second immunization with a high ag dose was stu ... | 1985 | 3996709 |
| pkx, the causative agent of proliferative kidney disease (pkd) in pacific salmonid fishes and its affinities with the myxozoa. | proliferative kidney disease (pkd), caused by an unclassified protozoan (pkx), is reported from pacific salmon, oncorhynchus tshawytscha (walbaum) and o. kisutch (walbaum), and steelhead trout, salmo gairdneri richardson, held at the mad river hatchery in california, usa. the cumulative mortality attributed to the disease was 95, 13, and 18% respectively. the mortalities were greatest at mean water temperatures of 12-14 degrees c during july 1983. the ultrastructure of the pkx organism and its a ... | 1985 | 4009511 |
| quaternary structure has little influence on spin states in mixed-spin human methemoglobins. | a key feature of the perutz stereochemical model for cooperativity in hemoglobin is a strong coupling between quaternary structure and the spin state of the heme iron [perutz, m. f. (1979) annu. rev. biochem. 48, 327-386]. while this coupling appears to be present for carp azide methemoglobin, it should also be present for all liganded forms of human methemoglobin that exhibit a thermal high-spin in equilibrium low-spin equilibrium. to test this hypothesis, we have measured the changes in spin e ... | 1985 | 4016083 |
| antibody diversity in fish. isoelectrofocalisation study of individually-purified specific antibodies in three teleost fish species: tench, carp and goldfish. | natural anti-dnp antibodies were isolated by affinity chromatography from individual sera of three cyprinid fish species (carp, goldfish and tench) and their electrofocusing (ief) spectra were analysed in reducing conditions. in addition, immune anti-penicillin and anti-bsa antibodies were isolated from individual and pooled tench sera, and studied by ief techniques on reduced samples. diversity rates appeared to be rather low in the three fish species, and striking similarities arose between in ... | 1985 | 4018343 |
| development and validation of a salmon prolactin radioimmunoassay. | a highly specific radioimmunoassay (ria) for the measurement of prolactin (prl) in the plasma and pituitary of salmonid fishes was developed using a rabbit antiserum to chinook salmon (oncorhynchus tschawytscha) prl. the prls purified from chinook salmon and chum salmon (o. keta) pituitaries showed exactly the same competitive inhibition curves in the ria, regardless of iodination of either hormone. the displacement curves for pituitary extracts and plasma from several salmonids, including chum, ... | 1985 | 4018564 |
| [metal concentrations in fishes of the rednitz river]. | we investigated 39 fish species (eel, brown trout, chub, carp bream, roach, perch, pike etc.) from the river rednitz (north bavaria) for content of the metals mercury, lead, cadmium, arsenic, selenium and zinc. the residue tolerance level for mercury (1 mg/kg) and the standard value of the federal public health office for lead (0.5 mg/kg) and cadmium (0.05 mg/kg) have not been exceeded in any case. because a small selenium concentration gives protection against the toxicity of mercury, we also h ... | 1985 | 4024745 |
| a comparative study of tropomyosin from vertebrate ventricles. | a comparative study of vertebrate ventricle tropomyosin has been carried out from the viewpoint of molecular evolution. the ventricles containing one-component tropomyosin were generally known, and in this paper those containing two components were also found in 8 species among mammals, reptiles, amphibia, and fish, but not among birds. the two components were concluded to be authentic tropomyosin and not artifacts since they showed lower electrophoretic mobilities in the presence of urea, and t ... | 1985 | 4030730 |
| [phylo- and ontogenic characteristics of lipid peroxidation in the retina of vertebrates]. | phylo- and ontogenetic aspects of lipid peroxidation and antioxidative enzyme system in the retina of vertebrates were studied. it was established that both the intensity of lipid peroxidation and the activity of glutathione peroxidase in the retina of different vertebrate animals (carp, frog, tortoise, pigeon, rabbit) considerably diminished with evolution. the differences in the intensity of lipid peroxidation and the activity of glutathione peroxidase between dark- and light-adapted retinas a ... | 1985 | 4052609 |
| ca2+-specific fluorescence changes in n-dansylaminoethyl-labelled oncomodulin. | rat oncomodulin (isoelectric point (pi) = 3.9) and homologous carp parvalbumin (pi = 3.95) was labelled at cys-18 which is located in the ab loop, with the fluorescent reagent n-dansylaziridine. the fluorescence spectrum of the labelled parvalbumin was insensitive to both mg2+ and ca2+ binding. in contrast the dansyl fluorescence emission spectrum of labelled oncomodulin was quenched approximately 20% by ca2+ in the presence of physiological concentrations of mg2+. approximately 90% of the ca2+- ... | 1985 | 4075234 |
| antigen localization in the lymphoid organs of carp (cyprinus carpio). | a brief morphological description is given of the spleen, head kidney and trunk kidney, the three peripheral lymphoid organs of carp. an antigen-localization study was carried out using aeromonas hydrophila (a cellular bacterial antigen) and an indirect immunofluorescence test. examination of the lymphoid organs at various times after injection (up to 12 months) showed that antigen was at first present in splenic ellipsoids and in solitary phagocytic cells in the spleen, head and trunk kidney. l ... | 1985 | 4075373 |
| spectroscopic studies of protein-heme interactions accompanying the allosteric transition in methemoglobins. | resonance raman, optical absorption, and circular dichroism spectroscopic techniques have been used to examine the effect of the addition of inositol hexaphosphate (ihp) to a series of carp and human methemoglobin derivatives. markers of spin equilibrium in the high-frequency region (1450-1650 cm-1) of the resonance raman spectrum yield high/low-spin ratios consistent with direct magnetic susceptibility measurements. changes in the low-frequency region (100-600 cm-1) of the resonance raman spect ... | 1985 | 4084499 |
| purification and partial characterization of the glutathione s-transferases in carp liver, and their interaction with 2,4-dichlorophenoxyacetic acid and 1,4-benzoquinone. | the hepatic glutathione s-transferase (gst) activity in the cytosol of the freshwater fish carp (cyprinus carpio) was enriched by glutathione affinity chromatography. the anionic (gst a1-a3) and cationic (gst c1-c3) isoenzymes were then separated in two chromatofocusing steps. sds electrophoresis showed gst c1 to be a heterodimer with subunits of mr 25,000 and 28,000, and all other isoenzymes to be homodimers with subunits of mr 25,400. they were partially characterized by different biochemical ... | 1985 | 4091850 |
| electrophoretic and immunological study of myosin light chains from freshwater teleost fishes. | white muscle myosin light chains from nine freshwater teleosts show a qualitative and quantitative variability on page without phylogenetic correlation. they look different from their higher vertebrate counterparts mainly with regard to electric charge and relative amounts of alkali light chains corresponding to various contents of isoenzymic forms of white muscle myosin. antibodies against carp white myosin lc1 recognize almost entirely white muscle lc1 from the other fishes and to a lesser deg ... | 1985 | 4092438 |
| ["competition of antigens" in carps (cyprinus carpio l.)]. | | 1972 | 4121719 |
| [studies on the antigenic similarity of immunoglobulins. i. cross reaction of carp immunoglobulins with immunoglobulins of representatives of various classes of vertebrates]. | | 1973 | 4126200 |
| the structure, composition and elastic properties of the teleost bulbus arteriosus in the carp, cyprinus carpio. | | 1973 | 4148163 |
| [structural and immunochemical studies on carp immunoglobulins (cyprinus carpio l.). i. analysis of the total molecule]. | | 1973 | 4200275 |
| fish rhabdovirus replication in non-piscine cell culture: new system for the study of rhabdovirus-cell interaction in which the virus and cell have different temperature optima. | the replication of three rhabdoviruses associated with diseases of fish has been demonstrated in cells of continuously cultivated non-piscine cell lines. spring viremia of carp (svc) virus and the salmonid fish viruses, egtved and infectious hematopoietic necrosis virus, all replicated in mammalian wi-38 (human diploid cell strain) and bhk/21 cells and in cells of one or more reptilian cell lines at the temperatures commonly used to propagate these viruses in fish cells. the infections were cyto ... | 1974 | 4210338 |
| [on the proof oa a protectin anti-e streptococcus cypr against glucose in the roe of cyprinus carpio l]. | | 1968 | 4233178 |
| [studies for the demonstration of sessile antibodies in the carp (cyprinus carpio l.). i. demonstration of sessile antibodies in carp immunized with horse serum]. | | 1968 | 4234956 |
| [studies concerning the demonstration of sessile antibodies in carp (cyprinus carpio l.). ii. demonstration of sessile antibodies to aeromonas punctata in carp adapted under natural conditions]. | | 1969 | 4239396 |
| [hemorrhagic septicemia of sliver carp]. | | 1974 | 4281147 |
| effects of cycloheximide and puromycin on synthesis of simian virus 40 t antigen in green monkey kidney cells. | gilden, r. v. (wistar institute, philadelphia, pa.), and r. i. carp. effects of cycloheximide and puromycin on synthesis of simian virus 40 t antigen in green monkey kidney cells. j. bacteriol. 91:1295-1297. 1966.-synthesis of the simian virus 40 (sv40) t antigen in primary african green monkey kidney cells was abolished when cycloheximide was added up to 10 hr postinfection. in contrast, puromycin, another inhibitor of protein synthesis, did not suppress antigen production. the basis of this di ... | 1966 | 4286450 |
| demonstration by immunofluorescence of the fixation of perfused antibody to human collagen in human kidney. | rabbit serum containing antibody to human collagen, perfused through human infant kidneys obtained at autopsy, gives an immunofluorescent reaction with an antigen in the basement membranes of the glomeruli and the tubules. this reaction was shown to be specific by the absence of reaction with normal rabbit serum, antibody to carp collagen, or anti-human collagen serum absorbed with human collagen. slight cross-reactions were found in the human kidneys with antibody to chicken or rat collagen. th ... | 1967 | 4289739 |
| [complementary study of the "developmental thermosensitivity" factor of poliomyelitis virus using the method of carp and koprowski]. | | 1967 | 4294745 |
| fish viruses: buffers and methods for plaquing eight agents under normal atmosphere. | a universal procedure was sought for plaque assay of eight fish viruses (bluegill myxovirus, channel catfish virus, eel virus, egtved virus, infectious hematopoietic necrosis virus, infectious pancreatic necrosis virus, lymphocystis virus, and the agent of spring viremia of carp (rhabdovirus carpio), in dish cultures of various fish cells. eagle minimal essential medium with sodium bicarbonate-co(2) buffer (earle's salt solution) was compared with minimal essential medium buffered principally wi ... | 1973 | 4349252 |
| [seeding of ponds and carp with clostridium perfringens bacilli]. | | 1972 | 4354424 |
| phosphate acceptor amino acid residues in structural proteins of rhabdoviruses. | partial acid hydrolysates of the [(32)p]phosphate- or [(3)h]serine-labeled proteins of purified vesicular stomatitis, rabies, lagos bat, mokola, or spring viremia of carp virions and of purified intracellular nucleocapsids of these viruses have been analyzed by paper electrophoresis for the presence of phosphorylated amino acids. both phosphoserine and phosphothreonine, with the former predominant, were present in virion and nucleocapsid preparations that contained phosphoproteins. an exception ... | 1974 | 4365328 |
| gyrodactylus kherulensis sp. n. (monogenoidea) from the carp. | | 1974 | 4448438 |
| [use of antibiotics in hemorrhagic septicemia of carp]. | | 1974 | 4450497 |
| [change in the composition of the fatty acids in hemorrhagic septicemia of carp]. | | 1974 | 4450498 |
| biosynthesis of proinsulin in islets of langerhans of the carp (cyprinus carpio). | | 1974 | 4604163 |
| the coordination of calcium ions by carp muscle calcium binding proteins a, b and c. | | 1974 | 4611157 |
| [reciprocal activity of hypothalamic factors of rams (ovis aries) and of teleostean fishes on the secretion in vitro of gonadotropic hormones c-hg and lh, respectively, by carp and ram hypophyses]. | | 1972 | 4624834 |
| comparison of thermal susceptibility and some other properties of hen and carp muscle amp-aminohydrolase. | | 1972 | 4659570 |
| [oral and parenteral, active immunization of carp against aeromonas punctata]. | | 1972 | 4665867 |
| [control of erysipelas in carp]. | | 1972 | 4667546 |
| natural occurrence of allocholic acid in ox and carp bile. | | 1972 | 4673765 |
| [in vitro glycogen phosphorylase activity of the carp (cyprinus carpio l.)]. | | 1973 | 4729446 |
| the amino acid sequence of rabbit skeletal muscle troponin c: gene replication and homology with calcium-binding proteins from carp and hake muscle. | | 1973 | 4797035 |
| [physiologic characteristics of the slime forming bacteria bacterium aceris (edson et carp.)]. | | 1974 | 4828754 |
| [comparative immunological studies by use of poikilothermal animals. first report: passive immunity of the carp employing the toxin of clostridium botulinus]. | | 1968 | 4871845 |
| the role of virus in septicemia of carp (cyprinus carpio) and pox of carp. influence of environment on infection. | | 1965 | 4957060 |
| [treatment of carp in khawia sinensis hsu infection]. | | 1971 | 5140684 |
| [blood changes in the carp following infection with aeromonas punctata]. | | 1971 | 5158662 |
| [biological tests in carp erysipelas]. | | 1971 | 5166698 |
| [septic pseudomonas in carp and silver carp]. | | 1971 | 5168331 |
| [experience in controlling carp erysepelas]. | | 1971 | 5170270 |
| investigation of the cause of the infectious dropsy (id) of carp. | | 1965 | 5220180 |
| etiology of infectious carp dropsy. | | 1965 | 5220598 |
| the rate of molecular evolution considered from the standpoint of population genetics. | the rate of amino acid substitutions in the evolution of homologous proteins is remarkably constant. furthermore, estimated rates of amino acid substitutions based on comparisons of the alpha hemoglobin chains of various mammals with that of the carp are about the same as those based on comparisons of the carp alpha and mammalian beta or the alpha and beta chains in mammals. these uniformities are regarded as evidence for the hypothesis that a majority of amino acid substitutions that occurred i ... | 1969 | 5260917 |
| immunologic relations among various animal collagens. | by the use of complement fixation and in vivo immunofluorescence with cross-absorption studies it was shown that acid soluble collagens prepared from rat, mouse, guinea pig, chicken, carp, and man exhibit species specificity. rat and mouse collagens were found to be indistinguishable and to cross-react with guinea pig collagen. cross-reactions also occurred between the collagens of rat and man and chicken and man. tissue specificity, or an antigen common to all of the collagens, was not demonstr ... | 1965 | 5320302 |
| enzymes of the pentose phosphate cycle in muscles of rat, ox, frog, lobster, chicken, northern pike and carp, and ehrlich ascites cells. | | 1969 | 5367359 |
| temperature effect on antibody production and immunological memory, in carp (cyprinus carpio) immunized against bovine serum albumin (bsa). | | 1969 | 5386638 |
| isolation and molecular properties of creatine kinase from carp white muscle. | | 1970 | 5490232 |
| the ultrastructure of carp (cyprinus carpio) immunoglobulin: a tetrameric macroglobulin. | | 1970 | 5492023 |
| normal stages in the development of cyprinus carpio var. communis l. | | 1970 | 5504578 |
| [studies on circular dichroism of some methemoglobin complexes of human, carp, and chironomus, as well as of horse myoglobin]. | | 1970 | 5530588 |
| [seasonal variations of the thyroxine effect on carp glucide metabolism]. | | 1971 | 5561570 |
| [study of the pathogenic properties of various strains of aeromonas and pseudomonas and their effect on healthy carp]. | | 1968 | 5720507 |
| yeast dosage level and the postembryonic survival and growth of the carp labeo rohita--ham. | | 1965 | 5884315 |
| [general considerations of the problem of the etiology of infectious dropsy of carp]. | | 1966 | 5963318 |
| the serologic specificity of tropocollagen telopeptides. | tropocollagen preparations from carp, buffalo fish, rats, calves, sheep, and humans have been studied by electron microscopy and serologic methods. tropocollagens from each species appeared identical by electron microscopy but they were readily distinguished (except between sheep and calves) by c'-fixation tests with rabbit antisera against the various tropocollagens. tests with calf tropocollagen antiserum showed no distinction between tropocollagen isolated from different tissues nor between i ... | 1967 | 6028490 |
| cloning, physical mapping and genome organization of mitochondrial dna from cyprinus carpio oocytes. | the mitochondrial genome from cyprinus carpio oocytes is a 10.5 megadalton, circular dna molecule. the carp mitochondrial dna was cloned in pbr325. three recombinant plasmids accounted for the entire genome. mapping of this dna using 11 different restriction endonucleases is reported here. both the large and small rrna genes were then localized using southern blot analysis. the subunit i of the cytochrome oxidase, the cytochrome b, the trnaglu and the urf 4 genes were localized by nucleotide seq ... | 1984 | 6090866 |
| synthesis of 4-substituted 2h-naphth[1,2-b]-1,4-oxazines, a new class of dopamine agonists. | a series of tricyclic oxazines, namely, the 4-substituted 2h-naphth[1,2-b]-1,4-oxazines, have been synthesized and assayed for dopamine agonist activity. one of the members of this series, compound (+)vii-15, was found to be a remarkably potent agonist in vivo when tested in the standard 6-hydroxydopamine lesioned rat assay. the absolute configuration of the compound corresponds to that found in the active isomer of apomorphine. its activity at the alpha 2 receptor (vs. [3h]clonidine) is relativ ... | 1984 | 6094811 |
| some properties of brain opioid receptors in membrane bound and solubilized state. | in brain membrane preparations, 3h-naloxone binding to bullfrog brain gave the highest values versus other species such as carp, chicken, rat and mouse. scatchard analysis revealed that the bmax value of bullfrog brain membranes was three fold greater than that of rat brain membranes. opioid receptors were solubilized from bullfrog brain membranes using digitonin or 3-(cholamidopropyl)-dimethylammonio-1-propane sulfonate (chaps) as the detergent. the properties of solubilized opioid receptors we ... | 1984 | 6099495 |
| the effect of temperature on the inhibition of trout, carp and human red cell hexokinase by triethyltin bromide. | | 1981 | 6118226 |
| the photoresponses of structurally identified amacrine cells in the turtle retina. | intracellular recordings were obtained from amacrine cells afterwards identified morphologically by horseradish peroxidase injection. there is a correlation between the time course of the photoresponses and the distribution of the cell processes across the inner plexiform layer (i.p.l.). cells producing the shortest duration, transient 'on-off' photoresponses branched in a single, narrow stratum of the i.p.l. (3-7 microns across). transient photoresponses with a longer time course were recorded ... | 1982 | 6127689 |
| a cell-free soluble-phase radioimmunoassay for thy-1 antigen. | a cell-free, soluble-phase, radioimmunoassay has been developed for thy-1 antigen. the method is based on immunoprecipitation of radiolabelled thy-1 molecules with specific antibodies, anti-immunoglobulin serum and polyethyleneglycol (peg). the method can be used with convenience to screen for the presence of thy-1 in various fluids as well as on cell surfaces for qualitative or quantitative purposes. presence of antibodies or autoantibodies against thy-1 can also be detected specifically. evide ... | 1983 | 6140218 |
| interaction of the component enantiomers of the putative dopamine autoreceptor agonist, tl-99 (6,7-dihydroxy-2-dimethylamino tetralin) with dopaminergic systems in mammalian brain and teleost retina. | the enantiomers of the putative dopamine autoreceptor agonist, tl-99 (6,7-dihydroxy-2-dimethylaminotetralin) were examined in a number of in vivo and in vitro test paradigms to further examine the reported autoreceptor selectivity of this compound. the (+)-isomer of the aminotetralin was more active as a dopamine agonist than either the racemate or the (-)-enantiomer. in addition to this dopaminergic activity, tl-99 was found to be a potent alpha 2-adrenoceptor agonist, this activity being more ... | 1983 | 6141532 |
| [comparative characteristics of the properties of erythrocyte na,k-atpase in man and the carp cyprinus carpio]. | studies have been made on some of the properties of na,k-atpase of a nuclear erythrocytes of man and nuclear erythrocytes of the carp cyprinus carpio. human erythrocytes yielded the enzymic activity only after their treatment by a detergent twin-20; under optimal conditions, it amounted to 2.6 mcmole /ml of erythrocytes per 1 h. in carp erythrocytes, na,k-atpase activity could be detected without detergent treatment, being 10-fold higher under optimal conditions than that in human erythrocytes. ... | 1984 | 6145272 |
| [evolutionary origin of the t-lymphocyte receptor. ii. production and partial characterization of antisera to the immunoglobulin-like cell membrane protein from thymocytes of the carp]. | an immunoglobulin-like protein from triton x-100 solubilized cell surface proteins from thymocytes can be isolated by specific precipitation using anti-igm antibodies. immunizing rabbits with those immune complexes we succeeded to elicit anti-thymocyte mig sera reacting with both thymocyte mig and igm. sds-page analysis revealed that anti-igm and anti-thymocyte mig sera recognize the same thymocytic surface protein. the cross-reactivity between thymocyte mig and igm is caused by protein determin ... | 1980 | 6163269 |
| substance p activity in the bullfrog retina: localization and identification in several vertebrate species. | immunoreactive substance p is present in the bullfrog retina, possibly in two types of stratified amacrine cells, with their somas in the inner nuclear layer and their neuronal processes entering the inner plexiform layer and ramifying in sublayers 3 or 4 (or both). occasionally, polygonal somas positive for substance p were found in the ganglion cell layer. approximately 75 percent of the cell bodies positive for substance p and 65 percent of the radioimmunoassayable substance p were found in t ... | 1981 | 6165081 |
| correspondence of homologies in amino acid sequence and tertiary structure of protein molecules. | according to the method developed previously (kubota, y., takahashi, s., nishikawa, k. and ooi, t. (1981) j. theor, biol. 91, 347-361), homology among proteins may be estimated quantitatively. we extended the method to investigate the relationship of an amino acid sequence to its teritary structure and identify homologous segments which have homologous native conformations in proteins. first, we selected proper indices for the computation of correlation coefficients from 32 properties inherent t ... | 1982 | 6176273 |
| the effect of primary and secondary immunization on the lymphoid tissues of the carp, cyprinus carpio l. | mirror carp immunized with human gamma globulin (hgg) in freund's complete adjuvant (fca) show a proliferative response involving cells whose cytoplasm stains deep red with methyl green-pyronin (pyroninophilic cells). this response occurs particularly in the haemopoietic parenchyma of the pronephros and mesonephros. it peaks at week 3, with the formation of clusters of pyroninophilic cells in the pronephros. immunization with aeromonas salmonicida elicited a less intense pyroninophilic response ... | 1982 | 6177828 |
| localization of immune complexes and heat-aggregated immunoglobulin in the carp cyprinus carpio l. | in carp cyprinus carpio l. preimmunized with a protein antigen, human gamma globulin (hgg), rapid localization of antigen occurred in the splenic ellipsoids within 1 day of a secondary immunization given 8 weeks later. furthermore, after injection of immune complexes or heat-aggregated hgg, antigen was trapped more quickly in the pronephros than when antigen was injected alone. these results support the conclusion that in fish, as in homoiotherms, this form of antigen trapping is antibody-depend ... | 1982 | 6180972 |
| [tolerance induction and production of anti-idiotypic antisera in carp]. | tolerance in carps can be induced by intracardial injection of 10-30 mg of ultracentrifuged human gamma globulin (hggu), with strong individual variation being observable. out of 21 carps pretreated with 10 mg hggu, 10 animals showed 14 days after i.p. test immunization with 1 mg hggu a log2 anti-hgg titre of 1.55 +/- 0.60 (control group - log2 7.33 +/- 1.47), while 11 animals exhibited a log2 titre of 7.45 +/- 2.75. low doses of hggu (0.01-1.0 mg) led to stimulation of immune response. if 1-2 m ... | 1982 | 6183853 |
| ontogeny of the immune system in carp (cyprinus carpio l.). the appearance of antigenic determinants on lymphoid cells detected by mouse anti-carp thymocyte monoclonal antibodies. | mouse anti-carp thymocyte monoclonal antibodies were used to investigate the appearance of lymphoid cell determinants in young carp. determinants detected by clone wct 4 were present on thymocytes as soon as the thymus was visible histologically, day 4 after fertilization (a.f.) at 21 degrees c, but wct 4-positive lymphocytes were not present in the pronephros until day 7 a.f.. in contrast, determinants detected by monoclonal antibodies from clones wct 8 and wct 10 appeared on thymocytes from da ... | 1983 | 6196239 |
| sulfomucins and sialomucins in the mucus cells of the alimentary canal in an indian freshwater major carp, labeo rohita (hamilton): a fluorescence microscopic study. | the localization of sulfomucins and sialomucins present in the various types of mucus cells--secretory (smc) and mature non-secretory mucus cells (mmc) of the different regions of the alimentary canal in labeo rohita (ham.) has been studied by fluorescence microscopical techniques (viz., ferric alum coriphosphine--fac, coriphosphine thiazol yellow--cty and dische reaction--dr). high degree of fluorescence activity confirms the presence of relatively high amount of sulfomucins in the mucus cells ... | 1983 | 6199266 |
| radioimmunochemical studies on 7.8s and 5.7s duck immunoglobulins in comparison with fab and fc fragments of chicken igy. | in ducks two forms of low molecular weight ig's +), 7.8s and 5.7s, have been described. to compare their antigenicity with fab and fc fragments of chicken igy a double-antibody ria technique was used in which the binding of 125i-chicken igy or 125i-chicken igy (fc) to rabbit and carp anti-chicken igy (fab) and anti-chicken igy (fc) antibodies, respectively, was inhibited by duck 7.8s and 5.7s ig's. chicken igy and duck 7.8s ig ( igy -like protein) are highly cross-reactive with respect to their ... | 1984 | 6202568 |
| specific activity of carp antisera against beta 2-microglobulin (beta 2m) and evidence for a beta 2m homologue in carp (cyprinus carpio). | antisera against rabbit and human beta 2-microglobulin were produced in carp (cyprinus carpio). these antisera were found to be specific for beta 2m, and detected beta 2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. in addition, these antisera were also inhibited by an extract from oyster. the differences between the reactivity of these two antisera and the ability of the anti-rabbit beta 2m to distinguish between ... | 1984 | 6209173 |
| immunoreactive opsin in the pineal organ of reptiles and birds. | the presence of opsin was investigated with light microscopic immunocytochemistry in pinealocytes of reptiles and birds (emys orbicularis, pseudemys scripta elegans, lacerta agilis et viridis, gallus domesticus, columba livia, melopsittacus undulatus, serinus canaria, taeniopyga punctate). the outer segments of pinealocytes selectively bound antiopsin antibody as revealed by indirect immunocytochemical techniques, indicating the occurrence of a rhodopsin-like photopigment in these structures. th ... | 1982 | 6213109 |
| ouabain-insensitive mechanism of cell volume maintenance in the carp kidney. | 1) an ouabain-insensitive mechanism of cell volume maintenance, similar to that operating in the kidney tissue of mammals and in epithelial cells of the frog urinary bladder, has been demonstrated in the kidney tissue of carp (cyprinus carpio)--there is no significant swelling even after 4-hour incubation with 10(-4) mol/l-1 ouabain. 2) the mechanism is not affected by 2-hour incubation with sodium in the saline replaced with tris or choline and, unlike in rabbit kidney cortex, cannot be inhibit ... | 1982 | 6214809 |
| [effects of a gonadotropic hormone of teleost fish on the adenylate cyclase of rat ovary and on its stimulation by ovine gonadotropins]. | carp gonadotropin (cgth) stimulated adenylate cyclase from 20-24 day old (but not 16-18 day old) rat ovaries; its subunits were inactive. carp gth (but not its subunits) increased the apparent kd for ofsh but not for olh. these results suggest that cgth mainly interacts with fsh sensitive adenylate cyclase-receptor systems, with an efficiency about 6 x 10(3) times lower than ofsh itself. | 1982 | 6220787 |
| effect of synthetic lys-trp on adenosine triphosphatase activity of carp and rabbit myosin bs. | 1. with carp myosin b atpase, the acceleration effect was found at peptide concentration ranging between 1 nm and 1 mm with a maximum at 10 nm (30% acceleration) at the low myosin b concentration (0.2 mg/ml). at the high myosin b concentration (0.8 mg/ml) a maximum was observed at 1-10 mm (10% acceleration). 2. with rabbit myosin b atpase, the acceleration (10%) was found only at a microm of lys-trp in the case of low myosin b concentration (0.2 mg/ml). under the high concentration of rabbit myo ... | 1983 | 6221861 |
| morphological and biochemical studies on liver, kidney and gill of fishes affected by pesticides. | effect of a herbicide, paraquat (1,1'-dimethyl-4,4'-bipyridilium-dichloride), the fungicide copper sulphate, and zinc chloride was studied on the histological structure of liver, kidney and gill of three fish species with different feeding habits, viz.: a herbivorous, silver carp (hypophthalmichthys molitrix); an omnivorous, common carp (cyprinus carpio l.) and a carnivorous, sheatfish (silurus glanis l.). the organs were studied electron microscopically after fixation according to karnovsky. th ... | 1983 | 6230560 |
| aporphine enantiomers. interactions with d-1 and d-2 dopamine receptors. | the r(-) and the s(+) enantiomers of apomorphine (apo) and n-n-propyl norapomorphine (npa) interact with both the d-1 and the d-2 dopamine receptors. r(-)-apo, as well as r(-)- and s(+)-npa, stimulates the d-1 dopamine receptor in carp retina; s(+)-apo blocks this dopamine receptor. similarly, r(-)-apo, as well as r(-)- and s(+)-npa, stimulates the d-2 dopamine receptor in the intermediate lobe of the rat pituitary gland; s(+)-apo blocks the intermediate lobe d-2 receptor. the interactions betwe ... | 1984 | 6231468 |
| [a further confirmation of the high idiotypic specificity of carp anti-human myeloma protein sera]. | previous investigations about the specificity of carp anti-idiotypic sera could be confirmed by comparison with corresponding antisera of guinea pigs. 3 carp antihuman myeloma-protein sera rendered as idiotypic specific after an appropriate absorption were tested in the passive hemagglutination inhibition test. they showed no difference to the parallel tested guinea pig antisera. it can be assumed that between fish antibodies and antibodies of mammals no functional difference exists in respect o ... | 1984 | 6240937 |
| [glycogen content and activities of phosphorylase and glucose-6-phosphatase in the fast and slow muscles of representatives of different classes of vertebrates]. | studies have been made on glycogen content as well as on the activity of phosphorylase and glucose-6-phosphatase in fast and slow muscles from representatives of 6 classes of vertebrates (lampetra fluviatilis, cyprinus carpio, rana temporaria, rana ridibunda, emys orbicularis, hen, rat). glycogen level and glucose-6-phosphatase activity are either higher in slow muscles, or practically identical in both types of muscles (glucose-6-phosphatase is absent from the fast muscles of hens and rats). on ... | 1980 | 6247864 |
| demonstration of specific receptors of the rough endoplasmic membrane for the signal sequence of carp preproinsulin. | 1. evidence is given for the cotranslational processing in a wheat germ cell-free system of carp preproinsulin to proinsulin by dog pancreatic rough endoplasmic membranes stripped of ribosomes by edta. concomitantly with processing there is a translocation of the protein across the membrane as shown by the accessibility to proteases. conversion of preproinsulin to proinsulin occurs post-translationally only in the presence of detergent. 2. stripped rough endoplasmic membranes bind a significant ... | 1980 | 6249584 |
| [subunits of lutropins: an intrinsic gonadotropic activity of alpha and the role of beta in the determination of the zoological specificity of action]. | pieces of eel ovary were incubated in presence of various gonadotropic hormones or their subunits (alpha, beta) and cyclic adenosine monophosphate tissue concentration was measured. carp gonadotropin (c-gth) and a hybrid (bovine lutropin alpha-c-gth beta) exhibited a very similar, intense, dose-dependent activity in the range 0.002-1 microgram/ml. mammalian lutropins (lh) and their subunits became active in the range 1-100 microgram/ml: a strong stimulation (#30x) was attained with bovine lh alp ... | 1981 | 6263510 |
| electron microscopy of aminergic retinal neurons. | the synaptic connections of the dopaminergic retinal neurons of cynomolgus monkeys and rabbits were studied in the electron microscope. in the cynomolgus monkeys, a labelling method with 5,6-dihydroxytryptamine was used, whereas an autoradiographic technique with a monoamine oxidase inhibitor and (3h)-dopamine was used in the rabbits. the labelling method with 5,6-dihydroxytryptamine altered the normal ultrastructure of the dopaminergic neurons, but their synaptic structures were still easily re ... | 1982 | 6291309 |
| [interaction of spin-labelled phosphatidyl choline with ca-dependent atpase from sarcoplasmic reticulum]. | the interaction between 3-acyl-2-(6-doxylpalmitoyl) phosphatidyl choline used as a hydrophobic spin probe and ca2+-dependent atpase from sarcoplasmic reticulum membranes of rabbit and carp white skeletal muscles was studied. the spin label incorporation into atpase preparations was performed at initial steps of atpase isolation by incubating reticulum membranes with the spin probe in the presence of cholic acid. a comparison of the molecular mobility of the probe incorporated into atpase prepara ... | 1982 | 6297625 |
| carp preproinsulin cdna sequence and evolution of insulin genes. | the nucleic acid sequence of the preproinsulin cdna of carp (cyprinus carpio), cloned in the psti-site of pbr322 (1), has been determined. the sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. the nucleotide sequence confirms the previously established amino acid sequence of carp insulin (2) and determines those of the signal (21 aa 1) and c-peptide (35 aa 1). ... | 1983 | 6306593 |
| isolation, analysis of structure, synthesis, and biological actions of urotensin i neuropeptides. | the 41-residue neuropeptide urotensin i (ui), from the urophyses of two teleost fish species (cyprinus carpio and catostomus commersoni), was isolated and purified, and its amino acid sequence was determined and confirmed by synthesis of a fully active peptide. the ui peptide was found to be a close structural and biological homologue of the ovine hypothalamic corticotropin-releasing factor (crf) and the frog skin peptide sauvagine; ui is, therefore, a phylogenetic prototype of this group of pep ... | 1983 | 6313156 |
| poly(adp-ribose) synthetase associated to cytoplasmic structures of meiotic cells. | an active poly(adp-ribose) synthetase has been found to be associated to mitochondria of frog oocytes and of testis from various species. the enzyme was also found in association with the microsomal-ribosomes fraction of the testis. both enzymes are strongly inhibited by nicotinamide, thymidine, and theophylline, and they synthesize oligomers of adp-ribose of an average chain length of 2-6 residues. the apparent km for nad+ of the mitochondrial enzyme is 22 micro m which contrasts with the corre ... | 1983 | 6317634 |
| stimulation in vitro of estradiol secretion by the ovary of a cichlid fish, sarotherodon aureus. | the objective of the present study was to investigate the nature of the gonadotrophic stimulation of estradiol (e2) secretion by the ovary of a teleost fish in vitro. spontaneous output of e2 decreased to a low baseline after 3 hr of superfusion and increased to a maximal level 2-3 hr after the introduction of homologous pituitary extract (t-pe) or human chorionic gonadotrophin into the superfusion system. addition of the phosphodiesterase inhibitor, isobutyl methylxanthine (ibmx, 0.5 mm), into ... | 1984 | 6321293 |
| comparison of the primary sequence of spring viremia of carp virus m protein with that of vesicular stomatitis virus. | the nucleotide sequence of spring viremia of carp virus mrna coding for the matrix protein (m) has been determined from a complete dna copy cloned in the escherichia coli plasmid pbr322. the clone is 710 nucleotides long and, excluding the 3' homopolymeric end (representing the mrna polyadenylic acid sequence), encodes a protein of some 223 amino acids (25,600 da). the protein has more basic than acidic amino acids and no significant amino- or carboxy-terminal hydrophobic domains. the predicted ... | 1984 | 6324471 |
| synthetic competitive antagonists of corticotropin-releasing factor: effect on acth secretion in the rat. | polypeptide analogs of the known members of the corticotropin-releasing factor (crf) family were synthesized and tested in vitro and in vivo for enhanced potency or competitive antagonism. predictive methods and physicochemical measurements had suggested an internal secondary alpha-helical conformation spanning about 25 residues for at least three members of the crf family. maximization of alpha-helix-forming potential by amino acid substitutions from the native known sequences (rat/human and ov ... | 1984 | 6326264 |
| [test systems for biomonitoring based on membrane-bound enzyme complexes. iv. assessment of genotoxic effects in an ames test system with metabolic activation by the microsomal mono-oxygenases from fish livers]. | the study of mutagenic effect of 2-aminoantracene and benz(alpha)pyrene on salmonella triphimurium ta 100 in the ames test-system in the presence of postmitochondrial fractions s-9 from carp liver with 3-methylcholantrene induced by microsomal oxidation system has been carried out. the metabolic activity and cytochrome p450 contence in carp liver microsomes have been shown to concede considerably those in rats liver. but these characteristics are sufficient for the use of fraction s-9 from carp ... | 1984 | 6329328 |