| analysis of the culture-derived soluble babesia canis canis antigens derived from the polish strains of the parasites. | objective, material and methods: the aim of this study was to analyse the protein fractions of the soluble parasitic antigen (spa) from in vitro cultures of the native polish strains of babesia canis canis and to determine their immunogenicity through western blotting using the sera of dogs vaccinated with this antigen. | 2012 | 23242220 |
| increased ast/alt ratio in azotaemic dogs infected with babesia canis. | the ast/alt ratio was estimated in 182 dogs infected with babesia canis. among these dogs 65 had anaemia and 68 were azotaemic. student's t test was used to compare means of the ast/alt ratio in anaemic and non-anaemic dogs, and in azotaemic and non-azotaemic dogs (p < 0.05). the differences in ast/alt ratio between anaemic (1.52 +/- 1.15) and non-anaemic (1.76 +/- 1.34) dogs were statistically insignificant (p = 0.23), however, the comparison of ast/alt ratio between azotaemic (2.68 +/- 1.52) a ... | 2012 | 23214368 |
| prevention of transmission of babesia canis by dermacentor reticulatus ticks to dogs treated with an imidacloprid/flumethrin collar. | a group of 8 dogs was treated with an imidacloprid/flumethrin collar (seresto(®)) 28 days prior to infestation with adult dermacentor reticulatus ticks, infected with babesia canis. the ability of the collar to prevent transmission of b. canis in the treated group was compared to an untreated control group. all 8 dogs in the untreated control group became infected with b. canis parasites, which were detected in blood smears as early as day 6 post tick-application. all control dogs developed clin ... | 2013 | 23158840 |
| conflicting results of serological, pcr and microscopic methods clarify the various risk levels of canine babesiosis in slovakia: a complex approach to babesia canis diagnostics. | we have performed a survey of babesia canis prevalence within group of dogs living in southern and western slovakia. blood samples and sera from 217 dogs, including individuals suspected of having babesiosis, were examined by nested pcr-rflp, light microscopy and indirect fluorescence antibody test (ifat). the detection of b. canis dna revealed the highest number of infected dogs in the region of nové zámky, with 23 b. canis-positive blood samples (35.4%, n=65), followed by an area close to komá ... | 2013 | 23040770 |
| molecular detection and characterization of potentially new babesia and theileria species/variants in wild felids from kenya. | piroplasms frequently infect domestic and wild carnivores. at present, there is limited information on the occurrence and molecular identity of these tick-borne parasites in wild felids in kenya. in 2009, a pair of captive lions (panthare leo) was diagnosed with suspected babesiosis and mineral deficiency at an animal orphanage on the outskirts of nairobi, kenya. blood smears indicated presences of haemoparasites in the erythrocytes, however, no further investigations were conducted to identify ... | 2012 | 22796449 |
| seroprevalence of babesia canis infection in clinically healthy dogs from western romania. | serum samples from 197 clinically healthy dogs residing in the banat region, the western historical part of romania, were assayed by an indirect fluorescent antibody test for the presence of anti-babesia canis antibodies. overall, the seroprevalence was 19.8% (39/197). the percent of seropositive dogs in rural areas (28.4%; 19/67) was significantly higher (p < 0.05) compared to dogs living in urban areas (15.4%; 20/130). seroprevalence of b. canis infection in hunting dogs was also found to be s ... | 2013 | 22681255 |
| in-clinic laboratory diagnosis of canine babesiosis (babesia canis canis) for veterinary practitioners in central europe. | haematological changes in dogs and climatic conditions favourable for the vector may assist in the quick in-house diagnosis of canine babesiosis. | 2012 | 22526812 |
| use of a real time pcr for detecting subspecies of babesia canis. | this paper reports the development and use of a real time pcr for detection of babesia canis canis, b. canis rossi, and b. canis vogeli in endemic areas of brazil. the sequences of the internal transcribed spacer (its) of several organisms were aligned and five primers and four probes were designed for amplification of a fragment (around 125 bp) which differentiates subspecies of b. canis. blood samples collected from dogs living in farms in three distinct rural regions within the state of minas ... | 2012 | 22516643 |
| [the prevalence of babesia canis canis in marsh ticks (dermacentor reticulatus) in the saarland]. | an accumulation of autochthonous cases of canine babesiosis caused by babesia canis has been registered in a small animal clinic in the saarland since the beginning of 2006, some cases with fatal outcome. this study aims to contribute to the explanation of strong focal occurrence of infections with b. canis in this region.therefore, patient owners who had presented their dogs in the years 2006 and 2007 because of babesiosis and who had claimed not having left the saarland with their dogs at leas ... | 2012 | 22515037 |
| strong monovalent electrolyte imbalances in serum of dogs infected with babesia canis. | canine babesiosis is a systemic tick-borne protozoan disease caused by infection with parasites of the genus babesia. acid-base disorders and ion imbalances have been described in dogs infected with babesia rossi in south africa. in this paper, the authors describe changes to monovalent ion concentrations and calculated parameters of monovalent ions in 70 dogs naturally infected with b. canis, a species occurring in europe. hyponatraemia, hypokalaemia, hyperchloraemia, decrease of chloride gap, ... | 2012 | 22463923 |
| electrolyte level and blood ph in dogs infected by various 18s rna strains of babaesia canis canis on the early stage of babesiosis. | the purpose of the studies was to determine electrolyte disturbances and blood ph changes in dogs with babesiosis and possibly show a connection between the babesia (b.) canis strain causing the infection and the intensity of these irregularities. 40 animals (group 1) with early babesiosis and 40 healthy dogs (group 2) were studied and their blood ph and blood levels of potassium, chlorides; calcium and sodium were determined. at the same time, molecular typing of parasites was carried out to de ... | 2012 | 22372324 |
| structural and functional characterization of bc28.1, major erythrocyte-binding protein from babesia canis merozoite surface. | babesiosis (formerly known as piroplasmosis) is a tick-borne disease caused by the intraerythrocytic development of protozoa parasites from the genus babesia. like plasmodium falciparum, the agent of malaria, or toxoplasma gondii, responsible for human toxoplasmosis, babesia belongs to the apicomplexa family. babesia canis is the agent of the canine babesiosis in europe. clinical manifestations of this disease range from mild to severe and possibly lead to death by multiple organ failure. the id ... | 2012 | 22294693 |
| molecular and serological detection of babesia spp. in neotropical and exotic carnivores in brazilian zoos. | large and small piroplasms have been observed in the blood smears of various wild carnivores, but few studies utilizing molecular characterization have been done. the goal of this present study was to investigate the presence of babesia sp. by molecular and serologic techniques in exotic and neotropical carnivores maintained in captivity at brazilian zoos. blood and sera samples were collected from 146 brazilian wild felids, 21 exotic felids, 1 genet (genetta tigrina), 3 european wolves (canis l ... | 2011 | 22946386 |
| relation of antioxidant status at admission and disease severity and outcome in dogs naturally infected with babesia canis canis. | canine babesiosis is caused by species of the babesia genus and has become an emerging disease worldwide. to the authors' knowledge there are no reports in which antioxidants have been analyzed in different presentations of canine babesiosis or in which the prognostic value of antioxidants has been studied. the aim of this study was to evaluate whether oxidative stress could be related to the severity and outcome of canine babesiosis. for this purpose a profile consisting of four antioxidant bio ... | 2017 | 28438201 |
| a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli. | babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ... | 2017 | 28739301 |
| a pcr survey of vector-borne pathogens in different dog populations from turkey. | in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ... | 2017 | 28682765 |
| a survey of canine haemoprotozoan parasites from turkey, including molecular evidence of an unnamed babesia. | canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. a reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as hepatozoon canis. ten samples hybridized to the theileria/babesia and babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrec ... | 2017 | 28673460 |
| first molecular detection of piroplasm infection in pet dogs from gansu, china. | babesiosis, the hemolytic disease caused by babesia, which is a tick-transmitted obligate intraerythrocytic protozoan parasite. this disease is responsible for significant mortality and morbidity rates and enormous economic losses to the livestock industry in tropical and subtropical regions worldwide. in this study, blood samples were collected from 141 pet dogs from gansu, china, and analyzed for babesia or theileria spp. infection using specific pcr and sequencing based on 18s rrna gene fragm ... | 2017 | 28638376 |
| molecular identification of babesia spp. in naturally infected dogs of kerala, south india. | canine babesiosis is a tick borne haemoprotozoan disease caused by large and small intraerythrocytic apicomplexan piroplasms of the genus babesia spp. the clinical manifestations of the disease vary from inapparent subclinical form to hyperacute shock related haemolytic crisis. microscopic examination of blood smears from suspected dogs revealed babesia canis and b. gibsoni in 3.45 and 25.86 % of samples respectively. a seminested pcr based on previously published species specific primers target ... | 2017 | 28615859 |
| prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in croatia. | vector-borne pathogens (vbps) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. the aim of the present study was to investigate the seroprevalence against babesia canis, anaplasma phagocytophilum, borrelia burgdorferi sensu lato, leishmania infantum, dirofilaria immitis, and ehrlichia canis in dogs in croatia. we investigated 435 randomly selected apparently healthy dogs in 13 different locations of croatia for antibodies to b. ca ... | 2017 | 28448211 |
| eurasian golden jackal as host of canine vector-borne protists. | jackals are medium-sized canids from the wolf-like clade, exhibiting a unique combination of ancestral morphotypes, broad trophic niches, and close phylogenetic relationships with the wolf and dog. thus, they represent a potential host of several pathogens with diverse transmission routes. recently, populations of the eurasian golden jackal canis aureus have expanded into the western palaearctic, including most of europe. the aim of our study was to examine eurasian golden jackals from romania, ... | 2017 | 28410591 |
| occurrence and diversity of arthropod-transmitted pathogens in red foxes (vulpes vulpes) in western austria, and possible vertical (transplacental) transmission of hepatozoon canis. | red fox (vulpes vulpes) is the most abundant wild canid species in austria, and it is a well-known carrier of many pathogens of medical and veterinary concern. the main aim of the present study was to investigate the occurrence and diversity of protozoan, bacterial and filarial parasites transmitted by blood-feeding arthropods in a red fox population in western austria. blood (n = 351) and spleen (n = 506) samples from foxes were examined by pcr and sequencing and the following pathogens were id ... | 2017 | 28835291 |
| new wide dynamic range assays for quantification of anti-leishmania igg2 and iga antibodies in canine serum. | the aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-leishmania igg2 and iga antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-leishmania antibodies in leishmania-seronegative and seropositive dogs, determined by a commercial elisa assay, and between different clinical stages according to leishvet guidelines. the analytical validation showed that the assays had a go ... | 2017 | 28669382 |
| prevalence and distribution of borrelia and babesia species in ticks feeding on dogs in the u.k. | ticks were collected during march-july 2015 from dogs by veterinarians throughout the u.k. and used to estimate current prevalences and distributions of pathogens. dna was extracted from 4750 ticks and subjected to polymerase chain reaction and sequence analysis to identify borrelia burgdorferi sensu lato (spirochaetales: spirochaetaceae) and babesia (piroplasmida: babesiidae) species. of 4737 ticks [predominantly ixodes ricinus linneaus (ixodida: ixodidae)], b. burgdorferi s.l. was detected in ... | 2017 | 28846148 |
| an elisa for the early diagnosis of acute canine babesiosis detecting circulating antigen of large babesia spp. | babesia canis is the predominant babesia species in dogs in europe and is responsible for a severe and fatal disease. an increase in global pet tourism and a widening of the geographic distribution of the tick vector has led to the emergence of infections in areas where previously only imported cases have been reported. due to the potential for rapid and serious disease progression, direct parasite detection by stained blood smears and light microscopy or dna-based methods have traditionally bee ... | 2017 | 28807287 |
| acute-phase response in babesia canis and dirofilaria immitis co-infections in dogs. | babesia canis and dirofilaria immitis are emerging and geographically overlapping vector-borne pathogens in dogs. infection with b. canis leads to acute-phase response (apr) that can be mild to severe and results in either non-complicated or complicated forms of the disease. the aim of this study was to determine whether acute b. canis infection is more severe in dogs with underlying asymptomatic d. immitis infection. dogs of both sexes, different ages and breeds, with naturally occurring mono-i ... | 2017 | 28802807 |
| high-resolution melting pcr assay, applicable for diagnostics and screening studies, allowing detection and differentiation of several babesia spp. infecting humans and animals. | the goal of the study was to design a single tube pcr test for detection and differentiation of babesia species in dna samples obtained from diverse biological materials. a multiplex, single tube pcr test was designed for amplification of approximately 400 bp region of the babesia 18s rrna gene. universal primers were designed to match dna of multiple babesia spp. and to have low levels of similarity to dna sequences of other intracellular protozoa and babesia hosts. the pcr products amplified f ... | 2017 | 28795223 |
| genome-wide analysis of gene expression and protein secretion of babesia canis during virulent infection identifies potential pathogenicity factors. | infections of dogs with virulent strains of babesia canis are characterized by rapid onset and high mortality, comparable to complicated human malaria. as in other apicomplexan parasites, most babesia virulence factors responsible for survival and pathogenicity are secreted to the host cell surface and beyond where they remodel and biochemically modify the infected cell interacting with host proteins in a very specific manner. here, we investigated factors secreted by b. canis during acute infec ... | 2017 | 28611446 |
| emergence of babesia canis in southern england. | the united kingdom is considered free of autochthonous transmission of canine babesiosis although cases are reported in dogs associated with recent travel abroad. during the winter months of 2015/16, a cluster of cases of disease in dogs with signs suggestive of canine babesiosis were reported in harlow, essex. | 2017 | 28514953 |