| a vaccination procedure against trypanosoma cruzi infection in mice by nonspecific immunization. | | 1975 | 804010 |
| epidemiological pattern of chagas' disease in an endemic area of costa rica. | triatoma dimidiata was found in association with 34.6% of the houses in a representative town within the dispersion area of the insect. the mean density over the entire study period was 21.5 insects per house, but it tended to be lower in the majority of the houses. insects usually were associated with dirt floors, poor sanitary condition of dwellings, and piles of firewood. the infection rate of 3,276 insects for trypanosoma cruzi was 30.9%. the infection was demonstrated in several synanthropi ... | 1975 | 804266 |
| a direct agglutination test for leishmaniasis. | antigen suspensions of enzyme-treated, formalin-fixed promastigotes of three species of leishmania (l. donovani, l. tropica, and l. braziliensis) were evaluated by using the direct agglutination test with serum samples from healthy individuals and patients with confirmed cases of visceral and cutaneous leishmaniasis. high cross-reactivity was obtained with the three antigens in tests with leishmania sera. sera from patients with kala-azar were 61% to 96% positive when tested with all three antig ... | 1975 | 804268 |
| trypanosoma cruzi: immunoperoxidase antibody test for serologic diagnosis. | | 1975 | 804410 |
| trypanosoma lewisi and t. cruzi: effect of infection on gestation in the rat. | | 1975 | 804411 |
| antiparasitic nitroimidazoles. 8. derivatives of 2-(4-formylstyryl)-5-nitro-1-vinylimidazole. | a series of 33 thioacetals and hydrazones of 2-(4-formylstyryl)-5-nitro-1-vinylimidazole was prepared and examined for antitrypanosomal properties. the thioacetals were inactive as antitrypanosomal agents but three hydrazones derived from n-aminoguanidine, pyridylacethohydrazide chloride (girard reagent p), and dimethylaminoacetohydrazide (girard reagent d) displayed good activity against trypanosoma rhodesiense. | 1975 | 804551 |
| antiparasitic nitroimidazoles. 9. synthesis of some 2-(4-dialkylaminomethylstyryl)-and 2-(4-amidinostyryl)5-nitro-1-vinylimidazoles. | a series of 2-styryl-5-nitro-1-vinylimidazoles carrying alkylaminomethyl or amidino functions in the 4 position of the styryl ring was prepared and evaluated for antitrypanosomal activity in mice infected with trypanosoma rhodesiense. the alkylaminomethyl compounds were found inactive against trypanosoma cruzi infections in mice. one compound, 2-(4-methylamidinostyryl)-5-nitro-1-vinylimidazole hydrochloride, showed antitrypanosomal activity comparable to the standard drugs suramin, pentamidine, ... | 1975 | 804556 |
| trypanosoma cruzi: early immune responses in infected mice. | | 1975 | 805059 |
| the immunology of experimental chagas' disease. ii. delayed hypersensitivity to trypanosoma cruzi antigens. | homogenates of suspensions of the trypomastigote and amastigote forms of the ernestina strain of trypanosoma cruzi, derived from tissue cultures, yielded two subcellular fractions which elicited strong delayed hypersensitivity reactions in rabbits. the 100,000 g times 90 minute fraction of t. cruzi homogenates gave rise to marked cell-mediated immunity. the 30,000 g times 35 minute fraction of these homogenates was also capable of eliciting a marked cell-mediated immune response. cell-mediated i ... | 1975 | 805088 |
| the influence of gonadectomy of host on parasitemia and mortality of mice infected with trypanosoma cruzi. | male cf1 mice were more susceptible to acute infections of trypanosoma cruzi than female mice as evidenced by significantly greater maximum mean parasitemia of approximately 8.9 times 10-6 per ml in males as compared to 1.5 times 10-6 per ml in females. mortality was also greater in males (80 to 90% as compared to 28% in females). ovariectomy of female mice made them more susceptible than unoperated female of similar age and stock to the brazil strain of t. cruzi as indicated by maximum mean par ... | 1975 | 805227 |
| [acute leukomia and chagas' disease. 4 cases]. | | 1975 | 805335 |
| killing in vitro of trypanosoma cruzi by macrophages from mice immunized with t. cruzi or bcg, and absence of cross-immunity on challege in vivo. | peritoneal macrophages from t. cruzi-immune mice were resistant to infection in vitro with culture forms of the parasite. macrophage resistance appeared in infected mice about 21 days postinfection when parasitemia was still rising. resistance in vitro was nonspecific since macrophages from bcg-immune mice were resistant to t. cruzi, and since macrophages from t. cruzi-immune mice were resistant to infection in vitro with listeria were not resistant to challenge with t. cruzi even when the par ... | 1975 | 806649 |
| letter: in vitro delayed hypersensitivity in chagas's disease (chronic and acute). | | 1975 | 806998 |
| studies on the selective lysis and purification of trypanosoma cruzi. | the mechanism by which culture forms of trypanosoma cruzi are lysed by normal mammalian sera was examined. lysis is restricted to the epimastigote form of the organism and is not dependent on the presence of agglutinins. lysis is a complement-dependent process, the activity being generated by the alternate pathway. the selective lysis by serum was exploited to purify viable trypomastigotes by means of centrifugation in an albumin column. essentially pure trypomastigote populations are now being ... | 1975 | 807672 |
| antigenic differences among epimastigotes, amastigotes and trypomastigotes of trypanosoma cruzi. | antigenic differences were demonstrated among trypomastigotes, amastigotes, and epimastigotes of trypanosoma cruzi by the indirect fluorescent antibody method. tests using cross-absorbed sera were included in the study. | 1975 | 807718 |
| a new liquid medium for trypanosoma (schizotrypanum) cruzi. | a new liquid medium, with fetal calf serum as the sole undefined component, was devised for the cultivation of trypanosoma cruzi. the need for the serum is ascribed to its mitogenic proteins, which stimulate division of, and the uptake of [3-h-] thymidine by the parasites. in the new medium, t. cruzi has a cycle culminating in the apperance of up to 90 per cent metacyclic forms in the stationary phase. this cycle is repeated on each serial transfer. | 1975 | 807720 |
| [urinary catecholamines excretion in experimental rat chagas' disease (levels under basal conditions, after insulin hypoglycemia and under reserpine action (author's transl)]. | | 1975 | 807956 |
| the immunology of experimental chagas' disease. iv. production of lesions in rabbits similar to those of chronic chagas' disease in man. | eight rabbits that received a single inoculation of trypomastigotes of a virulent strain of trypanosoma cruzi first developed a transient acute illness associated with parasitemia; later, 4 of these rabbits died with chronic myocarditis and/or with megacolon in the absence of demonstrable parasitemia or encysted parasites in tissues. two of these rabbits with chronic myocarditis died unexpectedly. three of the inoculated rabbits that survived the infection were sacrificed 18 months later and s ... | 1975 | 808136 |
| a rapid slide flocculation test for the diagnosis of american trypanosomiasis using trypanosoma cruzi fragments preserved by lyophilization. comparison with hemagglutination, immunofluorescence, and complement fixation tests. | a slide flocculation test for chagas' disease is described, which uses a lyophilized, stable antigen obtained by formalin and ultrasonic treatment of culture forms of trypanosoma cruzi. the test was compared with other tests for the serodiagnosis of american trypanosomiasis and showed a high sensitivity, positive results being obtained in every case of acute or chronic chagas' disease. in sera from blood donors and from normal individuals with negative t. cruzi antigen complement fixation tests ... | 1975 | 808144 |
| chagas' disease in el salvador. | several studies on chagas' disease in el salvador have supplied important epidemiologic information, including the following: (1) rates of house infestation by triatomid vectors have ranged from 26.3 per cent to 100 per cent in various localities studied, higher rates being found in rural than in urban areas. (2) triatoma dimidiata and rhodnius prolixus are the two vectors of t. cruzi in el salvador, with t. dimidiata predominating in localities at elevations above 600 meters and r. prolixus in ... | 1975 | 808244 |
| the effect of bcg on the course of experimental chagas' disease in mice. | | 1975 | 808508 |
| algorithms in the diagnosis and management of exotic diseases. iv. american trypanosomiasis. | | 1975 | 808578 |
| antiprotozoal thiazoles. 2-(5-nitro-2-thienyl)thiazoles. | a series of 2-(5-nitro-2-thienyl)thiazoles and their vinylogs having substituted methylamine side chains has been prepared by halogen displacement on the corresponding 4-chloromethylthiazole. of these, 4-morpholinomethyl-2-(5-nitro-2-thienyl)thiazole showed moderate activity against trypanosoma cruzi and trypanosoma rhodesiense in mice. this compound formed the lead for a series of analogous thiazole-4-carboxaldehyde hydrazones. some of the latter were found active in curing murine tryp. cruzi a ... | 1975 | 808611 |
| survey on experiences with latex-chagas-test in various countries. | the principle of the latex agglutination technique was applied on the serodiagnosis of chagas' disease. the latex particles coated with antigen from trypanosoma cruzi are agglutinated by antibodies against t. cruzi in the serum of patients suffering thereof. in 11600 comparative determinations worked out mainly in a number of south american laboratories, the sensitivity of this test was compared with xenodiagnosis, cf, ift and iha. the latex chagas test shows a mean coincidence of 90% with xenod ... | 1975 | 808885 |
| [study of kinetoplast dna of trypansoma cruzi using restriction endonucleases]. | the cleavage of kinetoplastic dna (kdna) with the restriction endonucleases (eco ri, hind ii + iii, hpa ii) was studied. the quantitative data of electrophoretically separated fragments show that the kdna (free circular genome units and complex network) is composed of distinct populations of circles. kinetic studies of renaturation of kdna previously cleaved by hpa ii into fragments having the length of one genome unit also indicate the presence of at least two and probably three populations of ... | 1975 | 809155 |
| trypanosoma cruzi: new foci of enzootic chagas' disease in california. | | 1975 | 809291 |
| resistance of trypanosoma cruzi to killing by macrophages. | | 1975 | 809716 |
| [silvane reservoirs and vectors of trypanosoma cruzi. 58. natural infection of the rat, akodon lasiotis (lung, 1841) by t. cruzi]. | | 1975 | 810875 |
| [trypanocidal activity of some ellipticine derivatives against trypanosoma cruzi cultured in vitro]. | some ellipticine derivatives showing antitumoral behaviour exhibit a trypanocide activity against in vitro cultures of t. cruzi. 9-methoxy-6-methyl-ellipticine is the most active compound of this series: its lethal effect is comparable with that of ethidium bromide. | 1975 | 811391 |
| enhancement of resistance and suppression of immunization against experimental trypanosoma cruzi infection by corynebacterium parvum. | intravenous but not intraperitoneal injection of killed corynebacterium parvum either before or after intraperitoneal infection with the highly reticulotropic tulahuén strain of trypanosoma cruzi produced enhanced resistance against the infection in mice. in contrast, c. parvum had no effect when the infection was caused with the predominately myotropic y strain of t. cruzi. c. parvum given intravenously before immunization with killed culture forms of the y strain parasite consistently diminish ... | 1975 | 811568 |
| chemotherapy of trypanosoma cruzi infections. | | 1975 | 812348 |
| immunologic and immunopathologic studies in congenital chagas' disease. | | 1975 | 812625 |
| depletion of heart norepinephrine in experimental acute myocarditis caused by trypanosoma cruzi. | the norepinephrine content of the heart was reduced to undetectable values in rats inoculated with trypanosoma cruzi. this fact indicates a massive involvement of the cardiac postganglionic sympathetic fibres in acute chagas disease. | 1975 | 812724 |
| enhanced mononuclear phagocytic activity during trypanosoma cruzi infection in mice. | it has been shown that trypanosoma cruzi-infected mice develop a nonspecific resistance to challenge with an unrelated microorganism, namely, listeria monocytogenes. this increased resistance to listeria was observed on the 4th day of trypanosoma infection and persisted for at least 25 days. it was associated with an increased mononuclear phagocytic activity, as revealed by carbon clearance experiments. the possibility that macrophages of the infected host become activated by a process which app ... | 1976 | 812831 |
| congenital chagas disease. | two different histological types of congenital chagas disease are defined. in one type, parasites were seen within the skeletal and cardiac fibers, and in the other, they are found mostly within the cells of the reticuloendothelial system. the latter was often associated with parasitized giant-cells with a single, lobulated, hyperchromatic nucleus. in 24 placentas, the most consistent findings were villous and intervillous inflammatory infiltrates. amastigotes of trypanosoma cruzi were found mos ... | 1976 | 813519 |
| [reservoirs and wild vectors of trypanosoma cruzi. lxi. behavioral aspects of various samples of t. cruzi in culture media]. | | 1975 | 814587 |
| [importance of synantropic animals in the control of endemic chagas' disease]. | | 1975 | 814610 |
| a comparison of rhodnius prolixus, triatoma infestans and panstrongylus megistus in the xenodiagnosis of a chronic trypanosoma (schizotrypanum) cruzi infection in a rhesus monkey (macaca mullatta). | fifth instar rhodnius prolixus, triatoma infestans and panstrongylus megistus were fed on a rhesus monkey (macaca mullatta) with a chronic infection of peru strain trypanosoma cruzi and examined 30 days later for the presence of trypanosomes in the rectum. no correlation could be demonstrated between either blood meal size or sex and the subsequent development of a rectal infection with t. cruzi. as t. infestans and p. megistus were more highly infected than r. prolixus in terms of both percenta ... | 1975 | 814658 |
| proceedings: preliminary observations on countercurrent immuno-electrophoresis of trypanosoma cruzi and leishmania. | | 1975 | 814659 |
| comparison of the complement fixation test and counterelectrophoresis test for the detection of antibodies in chagas disease. | sera from 452 patients and blood donors were tested for antibodies to tryp-anosoma cruzi. the complement fixation test and a counterelectrophoresis test were used. the results showed that both tests agreed in 92% of the sera. however, the counterelectrophoresis test was not as sensitive as the complement fixation test. | 1976 | 815268 |
| protective effects of specific antibodies in trypanosoma cruzi infections. | the action of immune sera from animals with chronic chagas disease on trypanosoma cruzi bloodstream forms (y and cl strains) was investigated. y strain trypomastigotes were agglutinated by anti-cl or anti-y immune sera and their infectivity was decreased when inoculated into normal mice. cl strain trypomastigotes, however, remained apparently unchanged, with neither agglutination nor decline of infectivity being observed after incubation with both immune sera. sera from three patients with chron ... | 1976 | 815433 |
| experimental chagas' disease: studies on the stability of a protective antigen. | | 1976 | 815532 |
| experimental chagas' disease: protective activity in relation with subcellular fractions of the parasite. | | 1976 | 815533 |
| cross-reactivity of lytic antibodies against blood forms of trypanosoma cruzi. | | 1976 | 815534 |
| [reaction of the trypanosoma cruzi strain to the experimental therapeutical response to bay 2502 (results of long term treatment)]. | | 1975 | 815983 |
| an immunocytotoxic assay for trypanosoma cruzi. | | 1976 | 816747 |
| novel heterocyclic nitrofurfural hydrazones. in vivo antirypanosomal activity. | hydrazine derivatives of several pyrazolo[1,5-alpha]pyrimidines (a), pyrazolo[1,5-alpha]-1,3,5-triazines (b), s-triazolo[1,5-alpha]pyrimidines (c), and imidazo[1,2-alpha]pyrimidines (d) were synthesized and condensed with 5-nitrofurfural in order to obtain the corresponding nitrofurfural hydrazones of each heterocycle (1d-14d). each compound was screened for in vitro activity against trypanosoma cruzi. the compounds were then tested in vivo against experimental infections of t. cruzi in laborato ... | 1976 | 817023 |
| mechanisms of resistance against experimental trypanosoma cruzi infection: the importance of antibodies and antibody-forming capacity in the biozzi high and low responder mice. | the role of antibodies and the host's antibody-forming capacity in resistance to trypanosoma cruzi infection has been investigated in the biozzi high and low responder lines of mice. ab/l animals with low antibody-forming capacity were found to be more susceptible to i.p. infection with trypomastigotes than high responders (ab/h), whereas non-selected swiss albino mice showed an intermediate level of susceptibility. the correlation between antibody-forming potentiality and susceptibility was con ... | 1976 | 818311 |
| the use of 51cr-labelled trypanosoma cruzi in agglutination titrations. | | 1976 | 819384 |
| inheritance of susceptibility of trypanosoma cruzi infection in rhodnius prolixus. | | 1976 | 819845 |
| [histopathological changes produced in mice by mexican strains of trypanosoma cruzi]. | | 1976 | 819978 |
| [wild reservoirs and vectors of cruzi. lx. attempts to cross rhodnius prolixus stal, 1859 and rhodnius neglectus lent, 1954 (hemiptera, reduviidae)]. | | 1976 | 819981 |
| trypanosome infections in the marmoset (saguinus geoffroyi) from the panama canal zone. | from august 1973 through may 1974 a total of 148 marmosets (saguinus geoffroyi) were examined for blood parasites. parasites were detected in 93.2% of the monkeys. direct examination of blood revealed 82.4% infected with trypanosomes; trypanosoma cruzi was seen in 1.3% of the animals examined t. minasense in 52.7% and t. rangeli in 25%. however, the use of several diagnostic tests (direct microscopic examination, hemoculture, xenodiagnosis, and animal inoculation) in 15 marmosets revealed t. cru ... | 1976 | 820209 |
| immunization of mice with irradiated trypanosoma cruzi growth in cell culture: relation of numbers of parasites, immunizing injections, and route of immunization to resistance. | | 1976 | 821876 |
| evaluation of an autoimmune type antibody in the sera of patients with chagas' disease. | utilizing cryostat sections of mouse heart as antigen, antibodies in the sera of patients with chagas' disease were investigated for autoimmune-type interation. sera from 47 normal, 70 patients with various contagious diseases, 116 sera from persons with leishmaniasis, and 90 from persons with chagas' disease were evaluated. antibodies in 44% of the sera tested from persons from chile with xenodiagnostically proven infections of chagas' disease showed antibodies that interacted with the heart se ... | 1976 | 822145 |
| nonspecific resistance against trypanosoma cruzi enhanced by corynebacterium parvum. | | 1976 | 822147 |
| letter: antibodies to neurons in chronic chagas' disease. | | 1976 | 822542 |
| the epidemiology and household distribution of seroreactivity to trypanosoma cruzi in a rural community in northeast brazil. | the prevalence rates and household distribution of seroreactivity to trypanosoma cruzi by complement fixation (cf) and indirect immunofluorescent antibody methods were determined in a population of 1,087 persons living in a rural area endemic for chagas' disease in northeast brazil. there was a gradual rise in the rate of seropositivity to 60% by age 20. between ages 20 and 55 the prevalence rate remained at about 60%, but declined thereafter. the decline in the older age groups was not accompan ... | 1976 | 822736 |
| comparison of counterimmunoelectrophoresis (cie) with other serologic tests in the detection of antibodies to trypanosoma cruzi. | application of counterimmunoelectrophoresis (cie) in detection of antibodies against trypanosoma cruzi in sera of 180 subjects from an endemic area (bolivia), and 50 subjects from a nonendemic area (u.s.a.) was carried out, and the results were compared to ones obtained with latex agglutination (la) and indirect hemagglutination (iha). the optimal conditions for cie were determined to be as follows: barbitone buffer of ph 8.2 and ionic strength 0.05 m was used in the chambers and in preparing th ... | 1976 | 822737 |
| trypanosoma cruzi: lactate dehydrogenase isoenzymes and infections in mice. | | 1976 | 824154 |
| experimental trypanosoma cruzi infections in rhesus monkeys. ii. the early chronic phase. | information on the acute and early chronic phases of infection with trypanosoma cruzi in eight rhesus monkeys is reported with particular reference to the pattern of subpatent parasitaemia and serology. xenodiagnosis was superior to animal inoculation and blood culture in its capacity to detect subpatent parasitaemia. each monkey showed an individual, relatively constant pattern of this not related to the nature of the inoculum. levels of indirect fluorescent and indirect haemagglutinating antib ... | 1976 | 824765 |
| effect of chagasic sera on the rat isolated atrial preparation: immunological, morphological and function aspects. | an antibody reacting with the plasma membrane of working myocardial cells, skeletal muscle fibres, and endothelial cells (evi antibody) has been described in the sera of patients with chagas' disease. in the present study of rat isolated atrial preparations beating in ddifferent media, direct immunofluorescence and ultrastructural immunohistochemical procedures indicate that the antibody can interact with the living tissue, becoming fixed to the plasma membranes. transmission electronmicroscopy ... | 1976 | 825225 |
| role of activated macrophages in resistance of mice to infection with trypanosoma cruzi. | the role of the activated macrophage in resistance of mice to infection with trypanosoma cruzi was investigated with use of mice that had not been infected with t. cruzi but whose macrophages were activated by toxoplasma gondii or besnoitia jellisoni. mice with activated macrophages were significantly more resistant to intra-peritoneal challenge with the tulahuen strain of t. cruzi than were controls. the role of serum factors in resistance to t. cruzi was excluded by the demonstration of no dif ... | 1976 | 826595 |
| the attraction of trypanosoma cruzi to vertebrate cells in vitro. | a video technic is described that permits a quantification of the degree of attraction of trypanosoma cruzi trypomastigotes to vertebrate cells in vitro. bovine embryo skeletal muscle cells (besm), hela cells and vero cells all attract a myotropic strain of t. cruzi trypomastigotes. besm cells, however, are 2-fold more attractive to trypomastigotes than hela cells and 10-fold more attractive than vero cells. heat-inactivation of besm cells abolishes their ability to respire and also to attract t ... | 1976 | 826623 |
| effect of fetal calf serum fractions and proteins on division and transformation of trypanosoma (schizotrypanum) cruzi in vitro. | division and epimastigote-to-trypomastigote transformation of trypanosoma cruzi were observed in o'daly's sm medium supplemented, in place of whole fetal calf serum, with fractions of this serum, its partially purified proteins, or with mixtures of these fractions and proteins. in addition to their division-promoting effects, most but not all serum fractions stimulated [3h]thymidine uptake by the flagellates. as revealed by teae-cellulose column chromatography and immunoelectrophoresis, the seru ... | 1976 | 826625 |
| the protective effect of the epimastigote forms of the pf strain of trypanosoma cruzi against a virulent homologous infection. | epimastigotes are assumed to be non-infective forms of trypanosomes. however, epimastigotes of the pf strain of the trypanosoma cruzi when injected in mice, in a single low dose, conferred on the animals a very good protection against challenge of a virulent homologous strain. | 1976 | 827037 |
| [1st finding of triatoma tibiamaculata pinto, 1926 naturally infected by trypanosoma cruzi in ubatuba, são paulo, brazil]. | | 1976 | 828291 |
| cellular immunity to trypanosoma cruzi infection in mice. | | 1976 | 828292 |
| immunology of chagas' disease i. circulating antigens in mice experimentally infected with trypanosoma cruzi. | | 1976 | 828294 |
| differences in the susceptibility of trypanosoma cruzi strains to active chemotherapeutic agents. | | 1976 | 828295 |
| [study on the use of rhodnius neglectus in xenodiagnosis in opossums (didelphis)]. | | 1976 | 828296 |
| [non-virulence in mice of pf branch of trypanosoma cruzi. evaluation through xenodiagnosis, tissue culture and histopathology]. | | 1976 | 829996 |
| [megavisceral syndrome. report of 2 cases]. | two patients with splanchnomegaly are presented, and it is proved through experimental, clinical, anatomicopathological, and epidemiological investigations that trypanosoma cruzi--the etiological agent of chagas' disease--causes splanchnomegaly in those countries where this disease is endemic. it is neither assured nor denied that trypanosome cruzi has been the causal agent of splanchnomegaly in the studied patients. it is suggested that in further investigations this problem may be cleared up. | 1976 | 830046 |
| [triatominae capable of transmitting trypanosoma cruzi in cuba]. | a brief review of the species of the genus triatoma that have been described in some epidemiology books is made. triatoma rubrofasciata has been found at the oriente province in our country. triatoma flavida has been found at the pinar del río, las villas, camagüey, and oriente provinces. both insects transmit the chagas' disease. | 1976 | 830048 |
| diagnosis of experimental encephalitozoonosis in rabbits by complement fixation. | a complement-fixation (cf) test has been developed for detection of experimental encephalitozoonosis in rabbits. the antigen consisted of disrupted homogenates of encephalitozoon cuniculi spores grown in and released from rabbit choroid plexus tissue culture cells. the test was sensitive and capable of detecting experimental encephalitozoonosis in rabbits as early as 15 days after intracerebral infection. the test was specific for infected animals, and no cross-reactivity was demonstrated betwee ... | 1977 | 864288 |
| investigation of the evi antibody in parasitic diseases other than american trypanosomiasis. an anti-skeletal muscle antibody in leishmaniasis. | the specificity of a circulating antibody observed in american trypanosomiasis and reacting with endocardium, blood vessels, and the interstitium of striated muscle (evi factor) was evaluated in the indirect fluorescent antibody test with 60 sera from patients with malaria, leishmaniasis, echinococcosis, amebiasis, african trypanosomiasis, toxoplasmosis, and trichinosis, collected from areas where chagas' disease is not endemic. two sera, 1 from a patient with plasmodium falciparum malaria and 1 ... | 1975 | 1089366 |
| studies of in vitro infection by trypanosoma cruzi. i. ultrastructural studies on the invasion of macrophages and l-cells. | the interactions of trypanosoma cruzi with l-cells, and with normal and activated macrophages in vitro were studied by ultrastructural techniques. t. cruzi actively invades cultured l-cells and uniformly destroys them. normal macrophages could control a 1:1 (parasite to host cell) infection, but were destroyed by a 10:1 infection. bcg-activated macrophages, however, controlled a 10:1 infection but not one at a ratio of 100:1. it appears that parasites that survive within host cells do so outsid ... | 1975 | 1089367 |
| trypanosoma cruzi: fatty acid metabolism in vitro. | | 1975 | 1090440 |
| the immunological response of cba mice to trypanosoma musculi: mechanisms of protective immunity. | | 1975 | 1090551 |
| axenic culture of trypanosoma cruzi in a chemically defined medium. | | 1975 | 1090715 |
| a macromolecule-free partially defined medium for trypanosoma cruzi. | a macromolecule-free medium, containing in its defined part 3 salts, glucose, hemin, 21 amino acids, 3 lipids, and some undefined components obtained by dialysis of liver infusion, was developed for serial cultivation of trypanosoma cruzi at 28 c. the medium allows prolonged cultivation of t. cruzi by serial transfers and growth comparable to that obtained in more complex media, including those containing blood serum. | 1975 | 1090736 |
| electron-microscopic mapping of at-rich regions and of e. coli rna polymerase-binding sites on the circular kinetoplast dna of trypanosoma cruzi. | partial alkaline denaturation of the circular kinetoplast dna (kdna) of trypanosoma cruzi has shown the existence of 4 small, well-defined at-rich regions with an average size of about 200 base pairs. they are almost equally distributed, separated by approximately 90 degrees on the circular molecule. all minicircles, whether free or linked in networks, have the same denaturation pattern and, therefore, seem to contain the same information. the long linear molecules present in low amounts in the ... | 1975 | 1092704 |
| glycoproteins from trypanosoma cruzi: partial purification by gel chromatography. | | 1975 | 1093868 |
| synthesis and antitrichomonal activity of certain pyrazolo (1,5-a) pyrimidines. | several bridgehead nitrogen heterocycles were synthesized to be screened as antimicrobial agents, modeled after nalidixic acid. the activity of these new compounds, all derivatives of 3-nitro-4,6-disubstituted pyrazolo (1,5-a)pyrimidin-7-ones (3,7,8, and 9), however, was found to be highly specific for trichomonas foetus and completely lacking in activity against bacteria, fungi, and parasites other than trichomonas. of the nine componds synthesized, including the intermediate 4,6-disubstituted ... | 1975 | 1094116 |
| the chondriome of selected trypanosomatids. a three-dimensional study based on serial thick sections and high voltage electron microscopy. | the unitary nature of the chondriome of two species of trypanosomatids, blastocrithidia culicis and trypanosoma cruzi, has been demonstrated by utilizing serial thick-sectioning techniques combined with high voltage electron microscopy. profiles of mitochondrial elements seen in thin sections and suspected to be parts of a continuum were confirmed by serial thick sectioning (0.25-0.50 mum thick) and stereopair analysis to be parts of the same mitochondrion. three-dimensional models obtained from ... | 1975 | 1095599 |
| letter: concomitant infection by toxoplasma gondii and trypanosoma cruzi. | | 1975 | 1096376 |
| an electron microscopic and cytochemical study of the cell coat of trypanosoma cruzi in tissue cultures. | using the cytochemical method of thiery, a polysaccharide surface coat was demonstrated with the electron microscope in the various developmental stages of trypanosoma cruzi maintained in tissue cultures. the cell coat was observed on the whole surface membrane system of t. cruzi. a positive reaction, similar to that observed in the cell membrane, was also obtained in the membranes of some vacuoles in the cytoplasm of the parasites. no granules were found in the parasites. no granules were found ... | 1975 | 1096482 |
| effect of the antiphagocytic agent cytochalasin b on macrophage invasion by leishmania mexicana promastigotes and trypanosoma cruzi epimastigotes. | unstimulated mouse peritoneal exudate cells were cultured on coverslips in medium 199 containing 10 per cent (v/v) calf serum. cytochalasin b dissolved in dimethyl sulphoxide (dmso) and diluted in medium 199 was added to cultures to give final concentrations of 1, 5 and 10 mug/ml. equal numbers of leishmania mexicana promastigotes, trypanosoma cruzi epimastigotes and sheep red cells were added to 24 hr cultures incubated at 37 c. the macrophage monolayers were fixed and stained at various time i ... | 1975 | 1097661 |
| cryopreservation of trypanosoma cruzi bloodstream forms. | recovery rates of t. cruzi bloodstream forms subjected to several methods of cryopreservation in liquid nitrogen and at -73 c are reported. inoculations of animals with cryopreserved and nonpreserved trypomastigotes revealed that prolonged storage at -196 c apparently did not change the biologic characteristics of different t. cruzi strains. the reproducibility and consistency of results suggest that "cryobanks" or "reference centers" may be established. | 1975 | 1099192 |
| [comparative morphological studies on the kinetoplast of various species of trypanosomes, with special reference to trypanosoma cruzi (author's transl)]. | comparative electron microscope studies on the morphology of the kinetoplast dna (k-dna) of the epimastigotes in many trypanosome species were carried out under standardized conditions. the k-dna shows a morphological variation during the cell cycle of culture forms of the trypanosome species under study. in longitudinal sections of the kinetoplast, the k-dna of t. cruzi appears as a compact trabecular structure; as a relatively disaggregated unit, with a central band or in a transitional stage ... | 1975 | 1099748 |
| a serological study on human trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay. | a micro-scale enzyme linked immunosorbent assay for african trypanosomiasis is described. the test uses very small quantities of sera and with t. brucei antigen is sensitive in detecting t. rhodesiense infections in man. cross reactions occur with antisera to, and antigens of, leishmania donovani and trypanosoma cruzi. | 1975 | 1099749 |
| trypanosoma cruzi: antimicrobial activity and strain differentiating properties of some five- and six-membered heterocyclic compounds on trypomastigotes. | | 1975 | 1100423 |
| trypanosoma cruzi: comparative fatty acid metabolism of the epimastigotes and trypomastigotes in vitro. | | 1975 | 1100424 |
| [silvan vectors and reservoirs of trypanosoma cruzi. lix. geografic distribution of triatominae in the rio grande hydrographic basin, brazil (hemiptera, reduviidae)]. | | 1975 | 1101317 |
| [ behaviour of a mexican strain of trypanosoma cruzi of particular virulence in the mouse]. | | 1975 | 1101332 |
| letter: trypanosoma cruzi--prenatal transfer of maternal antibody in man. | | 1975 | 1101461 |
| rat liver alcohol dehydrogenase isozymes: influence of infection with trypanosoma. | a study has been made of changes in alcohol dehydrogenase (adh) isozymes in livers of rats infected with the following trypanosomes: trypanosoma cruzi, t. rhodesiense, t. congolense, and six geographical isolates of t. lewisi. a control group of the same age was injected with saline, and another left uninjected. liver homogenates were electrophoresed in noble agar gels followed by formazan staining according to the method of ursprung and leone (1965). of a total of 25 replicates, 15 showed that ... | 1975 | 1101546 |
| epimastigotes and trypomastigotes of trypanosoma cruzi revealed by scanning electron microscopy. | epimastigotes and trypomastigotes of trypanosoma cruzi were observed by scanning electron microscopy. these two forms could easily be differentiated by examination of the undulating membrane and kinetoplast. subpellicular microtubules form parallel helices in epimastigotes while they are arranged parallel to the logitudinal axis in trypomastigotes. some epimastigotes and trypomastigotes showed a "knob-like" swelling at the end of their bodies. | 1975 | 1103885 |
| studies of ribosomal rna of trypanosoma cruzi. | | 1975 | 1104797 |
| serum proteins promoting [3h]thymidine uptake by trypanosoma (schizotrypanum) cruzi (chagas) in vitro. | five proteins capable of stimulating [3h]thymidine uptake by trypanosoma cruzi in vitro were isolated from fetal calf serum by (nh4)2so4 precipitation and ion exchange column chromatography. the proteins were partially characterized by immunodiffusion, immunoelectrophoresis, polyacrylamide gel disc electrophoresis, and sds electrophoresis. as estimated by sds electrophoresis, using 4 standards, the molecular weight of protein 1 was 100,000, that of protein 2 was 76,000, and that of proteins 3-5 ... | 1975 | 1104817 |