| a highly stable yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency. | yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human gi (gastrointestinal) tract have been published. stability of various y. lipolytica lipase powder formulations at various physiological ph values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. ... | 2010 | 20958263 |
| intracellular characterization of aerobic glucose metabolism in seven yeast species by 13c flux analysis and metabolomics. | key distinguishing characteristics of yeast glucose metabolism are the relative proportions of fermentation and respiration. crabtree-positive yeast species exhibit a respirofermentative metabolism, whereas aerobic species respire fully without secretion of fermentation byproducts. physiological data suggest a gradual transition in different species between these two states. here, we investigate whether this gradual transition also occurs at the intracellular level by quantifying the intracellul ... | 2011 | 21205161 |
| candida rugosa: a new fungal pathogen emerging, but from where? | | 2011 | 21212147 |
| improvement of catalytic activity of lipase from candida rugosa via sol-gel encapsulation in the presence of calix(aza)crown. | lipase from candida rugosa (crl) was encapsulated within a chemically inert sol-gel support in the presence of calix(aza)crowns as the new additives. the catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenyl palmitate (p-npp) and the enantioselective hydrolysis of racemic naproxen methyl ester. it has been observed that the percent activity yields of the calix(aza)crown based encapsulated lipases were higher than that of the free lipase. improved en ... | 2011 | 21256747 |
| in vitro activity of fluconazole and voriconazole against clinical isolates of candida spp. by e-test method. | the in vitro susceptibility of clinical candida isolates towards fluconazole and voriconazole was determined using the e-test method. a total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in kuala lumpur, malaysia were used. these comprised candida tropicalis, candida albicans, candida krusei, candida parapsilosis, candida rugosa, candida dubliniensis and candida glabrata. strains from american type culture collection were used as quality control. ... | 2010 | 20962716 |
| biochemical properties of free and immobilized candida rugosa lipase onto al(2)o(3): a comparative study. | abstract: lipase from candida rugosa (crl) was immobilized by physical adsorption onto al(2)o(3). the immobilization yield was more than 95% during 30 min. the properties of free and immobilized enzyme were also searched and compared. the optimum ph was shifted from 7 to 8.5 by immobilization of the enzyme. the maximum activity of the free and the immobilized enzymes occured at 35 and 40°c, respectively. | 2010 | 21117873 |
| candida rugosa: a possible emerging cause of candidaemia in trauma patients. | candida rugosa appears to be emerging as a distinctive cause of candidaemia in recent years. candidaemia due to this species is important to recognise because of its decreased susceptibility to azoles. | 2010 | 20658166 |
| conformational flexibility of lipase lip1 from candida rugosa studied by electronic spectroscopies and thermodynamic approaches. | we have used second-order orthogonal designs to obtain empirical models that describe the combined effect of ph and temperature on the secondary structure of a lipase (lip1) from candida rusosa. the equations that describe lipase conformational flexibility were derivated from the enzyme alpha helix fraction obtained from the experimental matrix. the thermal unfolding of lipase at different ph values was followed by measuring the circular dichroism signal as a function of temperature over a tempe ... | 2011 | 21318273 |
| selective biocatalytic aminolysis of (±)-epichlorohydrin: synthesis and icam-1 inhibitory activity of (s)-(+)-3-arylamino-1-chloropropan-2-ols. | regio- and enantioselective synthesis of (s)-(+)-3-arylamino-1-chloropropan-2-ols has been achieved by the epoxide ring opening of (±)-epichlorohydrin with different aromatic amines in the presence of candida rugosa lipase. activities of seven model (s)-(+)-3-arylamino-1-chloropropan-2-ols, out of 10 compounds synthesized, have been evaluated for the inhibition of tumor necrosis factor-a tnf-a) induced expression of intercellular adhesion molecule-1 (icam-1), which is one of the factors responsi ... | 2011 | 21419631 |
| mechanism of acetaldehyde-induced deactivation of microbial lipases. | microbial lipases represent the most important class of biocatalysts used for a wealth of applications in organic synthesis. an often applied reaction is the lipase-catalyzed transesterification of vinyl esters and alcohols resulting in the formation of acetaldehyde which is known to deactivate microbial lipases, presumably by structural changes caused by initial schiff-base formation at solvent accessible lysine residues. previous studies showed that several lipases were sensitive toward acetal ... | 2011 | 21342514 |
| immobilization of lipase from candida rugosa on sepabeads(®): the effect of lipase oxidation by periodates. | the objective of this paper was the investigation of a suitable sepabeads(®) support and method for immobilization of lipase from candida rugosa. three different supports were used, two with amino groups, (sepabeads(®) ec-ea and sepabeads(®) ec-ha), differing in spacer length (two and six carbons, respectively) and one with epoxy group (sepabeads(®) ec-ep). lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for m ... | 2011 | 21347667 |
| enantioselectivity of candida rugosa lipases (lip1, lip3, and lip4) towards 2-bromo phenylacetic acid octyl esters controlled by a single amino acid. | enantiomer discrimination by enzymes is a very accurate mechanism, which often involves few amino acids located at the active site. lipase isoforms from candida rugosa are very good enzymatic models to study this phenomenon as they display high sequence homology (>80%) and their enantioselectivity is often pointed out. in the present work, we investigated three lipases from c. rugosa (lip1, lip3, and lip4, respectively) towards the resolution of 2-bromo-arylacetic acid esters, an important class ... | 2011 | 21391204 |
| synthesis of terpinyl acetate by lipase-catalyzed esterification in supercritical carbon dioxide. | terpinyl acetate was successfully synthesized from alpha-terpineol and acetic anhydride in supercritical carbon dioxide (sc-co(2)) by enzymatic catalysis. five different lipases (candida rugosa type vii, amano ps, amano ap-6, amano g and lipozyme rm im) as biocatalysts for the terpinyl acetate synthesis were compared. an esterification extent of 53.0% was obtained in continuous operation using acetic anhydride as acyl donor and c. rugosa lipase as enzyme at 10mpa and 50 degrees c for 1.5h. tempe ... | 2010 | 20096575 |
| lipase-catalyzed synthesis of hydrophobically modified dextrans: activity and regioselectivity of lipase from candida rugosa. | vinyl decanoate-modified dextran macromolecules (dext40-vd) were synthesized in dimethyl sulfoxide at 50°c using lipase ay from candida rugosa for catalyzing transesterification between polysaccharide and vinyl fatty esters. the extent of dextran modification (quantified by the molar ratio of attached alkyl tails to sugar repeat units) with native-, ph-adjusted-, 18-crown-6 ether pretreated ph-adjusted-, and stepwise addition of pretreated lipase ay yielded <3%, 49%, 64% and 96% modified dextran ... | 2011 | 21602099 |
| c-terminal region of candida rugosa lipases affects enzyme activity and interfacial activation. | candida rugosa contains several lipase (crls) genes, and crls show diverse enzyme activity despite being highly homologous across their entire protein family. previous studies found that lip4 has a high esterase activity and a low lipolytic activity and lacks interfacial activation. to investigate whether the c-terminal region of the crls mediates enzymatic activity, chimeras were generated in which the c-terminus of lip4 from either residue 374, 396, 417, or 444 to residue 534 was swapped with ... | 2011 | 21504227 |
| improvement of catalytic properties of candida rugosa lipase by sol-gel encapsulation in the presence of magnetic calix[4]arene nanoparticles. | candida rugosa lipase (crl) was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane (teos) and octyltriethoxysilane (otes) in the presence of n-methylglucamine based calix[4]arene magnetic nanoparticles. the results indicate that the magnetic calix[4]arene based encapsulated lipase particularly has shown high conversion and enantioselectivity. it has also been noticed that the magnetic calix[4]arene based encapsulated lipase has excellent en ... | 2011 | 21509361 |
| engineering the expression and biochemical characteristics of recombinant candida rugosa lip2 lipase by removing the additional n-terminal peptide and regional codon optimization. | candida rugosa lipase (crl), an important industrial enzyme, has been established, containing several different isoforms which were encoded by the high-identity lip gene family (lip1 to lip7). in this study, we compared the expression and biochemical characterization with three different engineered lip2 constructions in the yeast pichia pastoris. our results showed that lip2 (lip2) has an overall improvement of 50% higher production yield (1.446 u/ml) relative to that of nflip2 (0.964 u/ml) at 7 ... | 2011 | 21561168 |
| cationic polyelectrolytes-lipases complexes formation as tool for recovery of these enzymes from their natural sources. | in the present paper the formation of complexes between positively charge polyelectrolyte (polyethyleneimine and chitosan) and candida rugosa lipase from a crude extract and porcine lipase from pancreas commercial homogenate preparations were analyzed. the solubility of lipases-cationic polyelectrolytes formation was dependent on: polyelectrolyte densities electrical charge, polyelectrolyte and enzyme concentrations and salts present in the solution. the lipase was recovered from the non-soluble ... | 2011 | 21640134 |
| expression and characterization of a novel propionyl-coa dehydrogenase gene from candida rugosa in pichia pastoris. | the propionyl-coa dehydrogenase (pacd) gene was firstly cloned from candida rugosa by the cdna race technique. the 6× his-tagged recombinant pacd gene was expressed in pichia pastoris gs115 and purified with ni-nta affinity chromatography. sds-page analysis and western blotting revealed that the molecular mass of the purified pacd was 49 kda. the results showed that the recombinant protein had the activity of catalyzing propionyl-coa to acrylyl-coa. the k (m), k (cat), and v (max) values of the ... | 2011 | 21960276 |
| epidemiology of candidaemia in critically ill trauma patients: experiences of a level i trauma centre in north india. | there is a need to understand the epidemiology and risk factors associated with candidaemia in critically ill trauma patients. the rise in incidence of non-albicans candidaemia and the emergence of antifungal resistance have made such a study necessary. a prospective laboratory-based surveillance study was performed over a period of 21 months (april 2008-december 2009) at a level i trauma centre in new delhi, india. all blood culture samples positive for candida were processed for microbial iden ... | 2010 | 21127153 |
| phenotypic and genotypic characterisation of two closely related subgroups of candida rugosa in the clinical specimens. | in this study, six clinical isolates (two from blood, two from urine, and one each from bronchoalveolar lavage and vaginal swab) were identified as candida rugosa based on carbohydrate assimilation profiles by api 20c aux and id32 kits. sequence analysis of the d1/d2 domain of the yeasts differentiated the isolates into two subgroups, a and b (three isolates per subgroup) which are closely related (99.1-99.6% similarity) to c. rugosa atcc 10571 strain. comparing to c. rugosa type strain, the its ... | 2011 | 21700741 |
| enhancement of activity and selectivity of candida rugosa lipase and candida antarctica lipase a by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil. | candida rugosa lipase (crl) and candida antarctica lipase a (cala) with improved activity and selectivity were prepared for use in organic solvent media. crl bioimprinted with fatty acids exhibited eightfold enhanced transesterification activity in hexane. combination of bioimprinting and coating with lecithin or with immobilization did not improve the activity further. cala was immobilized with and without bioimprinting, none of which improved the activity. all modified lipases were tested for ... | 2011 | 21695486 |
| Lipases are soluble and active in glycerol carbonate as a novel biosolvent. | Glycerol carbonate was synthesized as biosolvent for the development of soluble enzymatic system. The effects of various reaction parameters on activity and stability of lipases were investigated using the transesterification of ethyl butyrate with n-butanol as a model reaction. Enzymatic activity in glycerol carbonate was compared with that in water and in conventional organic solvents with different ionizing and dissociating abilities. The pK(a) value of trichloroacetic acid and transesterific ... | 2011 | 22112404 |
| Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis. | Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide. The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage. Following immobilization, CRL exhibited improved storage stability and performed better at higher incubation temperatures. In addition, the enzyme retained most of its catalytic efficiency after successive operational cycles. The immobilized derivative was also fully characterized with res ... | 2011 | 21876790 |
| immobilization of candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters. | in this work, mixed-mode silica particles functionalized with octyl and sulfonic acid groups was conveniently prepared by co-bonding a mixture of n-octyltriethoxysilane and 3-mercaptopropyltriethoxysilane and then oxidized with hydrogen peroxide. candida rugosa lipase (crl) was immobilized on the mixed-mode silica particles via hydrophobic and strong cation-exchange interaction. the resulting immobilized crl increased remarkably its stability at high temperature in comparison to free crl. the im ... | 2011 | 22209442 |
| identification of local clinical candida isolates using chromagar candida™ as a primary identification method for various candida species. | the objective of our study was to study the effectiveness of chromagar candida™ as the primary identification method for various clinical candida isolates, other than the three suggested species by the manufacturer. we studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 atcc strains. these strains were first cultured in sabouraud dextrose broth (sdb) for 36 hours at 35ºc, then on chromagar plates at 30ºc, 35ºc and 37ºc. the sensitivity of t ... | 2011 | 22041745 |
| short communication: epidemiology and genotyping of candida rugosa strains responsible for persistent intramammary infections in dairy cows. | the present study was undertaken during an outbreak of clinical and subclinical mastitis in 14 dairy cows caused by candida rugosa, in which high somatic cell counts were seen and cases did not respond to antibiotic treatment. intramammary infection cured spontaneously in 10 cows, whereas 4 cows were culled as a result of persistent infections. repeated sampling of these cows and biomolecular analysis of the isolates showed that the infections were caused by the same genotype, even over a period ... | 2011 | 21854930 |
| characterization of codon-optimized recombinant candida rugosa lipase 5 (lip5). | recombinant candida rugosa lipase 5 (lip5) has been functionally expressed along with other isoforms in our laboratory. however, the characterization and codon optimization of lip5 have not been done. in this work, we characterized, codon-optimized and compared lip5 with commercial lipase. lip5 activity on hydrolysis of p-nitrophenyl (p-np) butyrate was optimal at 55 °c as compared with 37 °c of the commercial lipase. several assays were also performed to determine the substrate specificity of l ... | 2011 | 21854055 |
| novel magnetic microspheres of p (gma-b-hema): preparation, lipase immobilization and enzymatic activity in two phases. | magnetic oleic-acid-coated fe(3)o(4) nanoparticles were first introduced into 1, 1-diphenylethylene (dpe)-controlled radical polymerization system to prepare superparamagnetic microspheres for enzyme immobilization by two steps of polymerization. in the presence of dpe, glycidyl methacrylate, 2-hydroxyethyl methacrylate and methacryloxyethyl trimethyl ammonium chloride with charge were selected as copolymering monomers based on their reactive functional group and excellent biocompatibility which ... | 2011 | 22159608 |
| chemoenzymatic synthesis of phytosteryl ferulates and evaluation of their antioxidant activity. | the feasibility of a two-step chemoenzymatic synthesis of phytosteryl ferulates was successfully established in this work. an intermediate vinyl ferulate was first chemically produced and subsequently esterified with phytosterols through alcoholysis with candida rugosa as a catalyst. the structures of phytosteryl ferulates were confirmed by fourier transform infrared (ftir) and high-performance chromatography-mass spectrometry/mass spectrometry (hplc-ms/ms) using atmospheric pressure chemical ... | 2011 | 22023159 |
| Selection of orlistat as a potential inhibitor for lipase from Candida species. | Infections caused by Candida species manifest in a number of diseases, including candidemia, vulvovaginal candidiasis, endocarditis, and peritonitis. Candida species have been reported to possess lipolytic activity due to the secretion of lipolytic enzymes such as esterases, lipases and phospholipases. Extra-cellular hydrolytic enzymes seem to play an important role in Candida overgrowth. Candidiasis is commonly treated with antimycotics such as clotrimazole and nystatin. The antimycotics bind t ... | 2011 | 22125382 |
| immobilization of candida rugosa lipase on poly(3-hydroxybutyrate-co-hydroxyvalerate): a new eco-friendly support. | the overall objective of this study is to evaluate the morphological [scanning electron microscopy (sem)], physicochemical [differential scanning calorimetry (dsc), thermogravimetric analysis (tga), chemical composition analysis, fourier-transform infrared spectroscopy (ftir), nuclear magnetic resonance (nmr)], and biochemical properties of candida rugosa lipase (crl) immobilized on a natural biopolymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (phbv) in aqueous solution. crl was immobilized by ... | 2011 | 21870100 |
| phyto-inspired silica nanowires: characterization and application in lipase immobilization. | silica nanostructures were phyto-fabricated on different surfaces by using pomegranate (punica granatum) leaf extracts. on zinc films, nanowires were obtained. on other surfaces such as silica, alumina, zinc oxide and glass, spherical aggregates, cubic assemblies, micro-flakes and acicular structures, respectively, were observed. the nanowires developed on zn surfaces were characterized by scanning electron microscope-energy dispersive spectrometer (sem-eds), x-ray diffraction (xrd), photolumin ... | 2011 | 22201456 |
| taxonomic and molecular characterization of lactic acid bacteria and yeasts in nunu, a ghanaian fermented milk product. | produced from raw unpasteurized milk, nunu is a spontaneously fermented yoghurt-like product made in ghana and other parts of west africa. despite the importance of nunu in the diet of many africans, there is currently only limited information available on the microorganisms associated with nunu processing. with the aim of obtaining a deeper understanding of the process and as a first step towards developing starter cultures with desired technological properties for nunu production, a microbiolo ... | 2012 | 23541194 |
| enantioselective enzymatic hydrolysis of racemic drugs by encapsulation in sol-gel magnetic sporopollenin. | candida rugosa lipase was encapsulated within a sol-gel procedure and improved considerably by fluoride-catalyzed hydrolysis of mixtures of octyltriethoxysilane and tetraethoxysilane in the presence of magnetic sporopollenin. the catalytic properties of the immobilized lipases were evaluated into model reactions, i.e., the hydrolysis of p-nitrophenylpalmitate (p-npp), and the enantioselective hydrolysis of racemic naproxen methyl ester, mandelic acid methyl ester or 2-phenoxypropionic acid methy ... | 2011 | 21932062 |
| A novel control of enzymatic enantioselectivity through the racemic temperature influenced by reaction media. | The influence of reaction media on the racemic temperature (T(r)) in the lipase-catalyzed resolution of ketoprofen vinyl ester was investigated. An effective approach to the control of the enzymatic enantioselectivity and the prediction of the increasing tendency was developed based on the T(r) influenced by reaction media. The T(r) for the resolution catalyzed by Candida rugosa lipase (CRL) was found at 29 °C in aqueous and S-ketoprofen was obtained predominantly at 40 °C. However, CRL showed R ... | 2011 | 22113016 |
| in silico and experimental characterization of chimeric bacillus thermocatenulatus lipase with the complete conserved pentapeptide of candida rugosa lipase. | lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. to provide chimeric bacillus thermocatenulatus lipase (btl2), the completely conserved pentapeptide (¹¹²ala-his-ser-gln-gly¹¹⁶) was replaced with similar sequences (²⁰⁷gly-glu-ser-ala-gly²¹¹) of candida rugosa lipase (clr) at the nucleophilic elbow region. for this purpose, three mutations including a112g, h113e, and q115a were inserted in the conserve ... | 2012 | 23274720 |
| mechanistic investigations of lipase-catalyzed degradation of polycarbonate in organic solvents. | the biodegradation of an engineering thermoplastic, poly (bisphenol-a carbonate) (bpapc), was carried out using three different lipases from candida antarctica (cal), candida rugosa (crl) and porcine pancreas (ppl) in water-miscible (tetrahydrofuran) and water-immiscible (chloroform) solvents for 10 days. the degradation was monitored by gel permeation chromatography and fourier transform infrared spectroscopy. maximum degradation (ca. 60% reduction in m(n)) of bpapc was observed in thf with ppl ... | 2011 | 22112773 |
| chemoenzymatic synthesis with lipase catalyzed resolution and evaluation of antitumor activity of (r/s)-2-[2-hydroxy-3-(4-phenylpiperazin-1-yl)propyl]-1h-pyrrolo[3,4-b]quinolin-3(2h)-one. | synthesis, characterization, resolution and evaluation of novel (r/s)-2-[2-hydroxy-3-(4-phenylpiperazin-1-yl)propyl]-1h-pyrrolo[3,4-b]quinolin-3(2h)-one derivatives are described. enantiomerically pure compounds were isolated in good to excellent yield with high enantiomeric excess under mild reaction conditions by using candida antarctica b (cal-b) and candida rugosa (crl) lipases. newly synthesized and resolved compounds were screened for their antitumor activity against cancer cells such as h ... | 2011 | 21440337 |
| lipase-immobilized biocatalytic membranes for biodiesel production. | microbial lipase from candida rugosa (amano ay-30) has good transesterification activity and can be used for biodiesel production. in this study, polyvinylidene fluoride (pvdf) membrane was grafted with 1,4-diaminobutane and activated by glutaraldehyde for c. rugosa lipase immobilization. after immobilization, the biocatalytic membrane was used for producing biodiesel from soybean oil and methanol via transesterification. response surface methodology (rsm) in combination with a 5-level-5-factor ... | 2012 | 23357586 |
| biochemical methane potential of raw and pre-treated meat-processing wastes. | raw and pre-treated greaves and rinds, two meat-processing wastes, were assessed for biochemical methane potential (bmp). combinations of temperature (25, 55, 70 and 120 °c), naoh (0.3 g g(-1) waste volatile solids) and lipase from candida rugosa (10 u g(-1) fat) were applied to promote wastes hydrolysis, and the effect on bmp was evaluated. cod solubilisation was higher (66% for greaves; 55% for rinds) when greaves were pre-treated with naoh at 55 °c and lipase was added to rinds after autoclav ... | 2012 | 23266854 |
| new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation. | the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ... | 2016 | 27236224 |
| recombinant candida rugosa lipase 2 from pichia pastoris: immobilization and use as biocatalyst in a stereoselective reaction. | the characterization of the recombinant candida rugosa lip2 (r-lip2) isoenzyme obtained from fed-batch cultures of pichia pastoris under paox promoter was carried out, determining the optimal ph and temperature as well as their catalytic performance in both hydrolysis and synthesis reactions comparing with purified native lip2 (n-lip2) previously determined. the substrate specificity of r-lip2 in hydrolysis reactions was determined with a series of triacylglycerols and p-nitrophenyl esters of va ... | 2010 | 20945483 |
| promotion of the lipase-catalyzed hydrolysis of conjugated linoleic acid l-menthyl ester by addition of an organic solvent. | conjugated linoleic acid l-menthyl ester was hydrolyzed in water by the lipase from candida rugosa with the addition of an organic solvent. the degree of hydrolysis (yield) greatly improved when a tertiary alcohol, such as t-butyl alcohol, was added. however, the addition of a less polar solvent, such as hexane, decreased the degree of hydrolysis, and some water-miscible solvents, such as acetone, caused inactivation of the lipase. with the addition of t-butyl alcohol, the reaction mixture forme ... | 2012 | 23420098 |
| pcr-sscp-based reconstruction of the original fungal flora of heat-processed meat products. | food processing of spoiled meat is prohibited by law, since it is a deception and does not comply with food safety aspects. in general, spoilage of meat is mostly caused by bacteria. however, a high contamination level of fungi could be also found in some meat or meat products with certain preserving conditions. in case that unhygienic meat is used to produce heat processed products, the microorganisms will be deactivated by heat, so that they cannot be detected by a standard cultivation method. ... | 2013 | 23361099 |
| maldi-tof mass spectrometry for rapid identification of clinical fungal isolates based on ribosomal protein biomarkers. | this study aimed to evaluate the identification of clinical fungal isolates (yeast and molds) by protein profiling using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof/ms). a total of 125 clinical fungal culture isolates (yeast and filamentous fungi) were collected. the test set included 88 yeast isolates (candida albicans, candida glabrata, candida guilliermondii, candida kefyr, candida krusei, candida parapsilosis, candida rugosa, candida tropicalis and ... | 2015 | 25541362 |
| demonic axe-like conjugated alkynes in combating microbes. | a new series of disubstituted alkynes was obtained by microwave induced internal splitting of the corresponding β-oxo-alkylidenetriphenylphosphoranes. the antimicrobial potential of these conjugated alkynes and phosphoranes was assayed in vitro against three gram-positive bacteria (staphylococcus aureus, bacillus subtilis, staphylococcus epidermidis), three gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae) and five fungal strains (aspergillus niger, candida ... | 2013 | 23973825 |
| expression and properties of three novel fungal lipases/sterol esterases predicted in silico: comparison with other enzymes of the candida rugosa-like family. | lipases from the candida rugosa-like family are enzymes with great biotechnological interest. in a previous work, several enzymes from this family were identified by in silico mining of fungal genomes. here, we describe the cloning, expression, and characterization of putative lipases from the genomes of nectria haematococca, trichoderma reesei, and aspergillus niger and compared their catalytic properties with those of ope, a well-characterized sterol esterase/lipase from ophiostoma piceae. all ... | 2015 | 26272094 |
| occurrence of mycotoxins and yeasts and moulds identification in corn silages in tropical climate. | this study was aimed to identify yeasts and moulds as well as to detect mycotoxin in corn silages in southern minas gerais, brazil. | 2016 | 26787003 |
| production of biodiesel from plant oil hydrolysates using an aspergillus oryzae whole-cell biocatalyst highly expressing candida antarctica lipase b. | for enzymatic biodiesel production from plant oil hydrolysates, an aspergillus oryzae whole-cell biocatalyst that expresses candida antarctica lipase b (r-calb) with high esterification activity was developed. each of soybean and palm oils was hydrolyzed using candida rugosa lipase, and the resultant hydrolysates were subjected to esterification where immobilized r-calb was used as a catalyst. in esterification, r-calb afforded a methyl ester content of more than 90% after 6 h with the addition ... | 2013 | 22850174 |
| microbiological and chemical characteristics of tarubá, an indigenous beverage produced from solid cassava fermentation. | the aim of this work was to identify and characterize the microbiota present during fermentation and in the final beverage, tarubá, by culture-dependent and -independent methods. in addition, target chemical compounds (carbohydrates, organic acids, and ethanol) were evaluated. lactic acid bacteria (lab) and mesophilic bacteria were the predominant microorganisms. among them, lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides, and bacillus subtilis were frequently isolated a ... | 2015 | 25846929 |
| cloning and transcription analysis of the candida rugosa propionyl-coa dehydrogenase gene and its expression in pichia pastoris. | the propionyl-coa dehydrogenase (pacd) gene was cloned from candida rugosa by the cdna race technique. the full cdna of the pacd gene has a length of 1408 bp, which contains a complete open reading frame (orf) of 1329 bp, coding for 442 amino acids. the cdna of pacd was cloned into the expression plasmid ppic9k and transformed into pichia pastoris gs115. the recombinant protein was purified by ni-nta affinity chromatography, and its size was observed to be approximately 49 kda as estimated by sd ... | 2011 | 21780146 |
| regioselective deprotection of the monosaccharide-bearing thiocyanomethyl group at the anomeric position monitored by reversed-phase hplc method. | in the current work, the investigation and development of a chemo-enzymatic approach for the synthesis of neo-glycoproteins have been studied. this strategy is based on the regioselective enzymatic hydrolysis of peracetylated monosaccharide, functionalized at the anomeric position (c1) as 1-thio-(s-cyanomethyl) group, a precursor of the 2- iminomethoxyethyl thioglycosides-linker for protein glycosylation, catalyzed by immobilized enzymes to obtain selectively monodeprotected compounds. the use o ... | 2016 | 26864255 |
| selective production of 9r-hydroxy-10e,12z,15z-octadecatrienoic acid from α-linolenic acid in perilla seed oil hydrolyzate by a lipoxygenase from nostoc sp. sag 25.82. | hydroxy fatty acids (hfas) derived from omega-3 polyunsaturated fatty acids have been known as versatile bioactive molecules. however, its practical production from omega-3 or omega-3 rich oil has not been well established. in the present study, the stereo-selective enzymatic production of 9r-hydroxy-10e,12z,15z-octadecatrienoic acid (9r-hote) from α-linolenic acid (ala) in perilla seed oil (po) hydrolyzate was achieved using purified recombinant 9r-lipoxygenase (9r-lox) from nostoc sp. sag 25.8 ... | 2015 | 26379279 |
| quantitative structure-activity relationship correlation between molecular structure and the rayleigh enantiomeric enrichment factor. | it was recently demonstrated that under environmentally relevant conditions the rayleigh equation is valid to describe the enantiomeric enrichment - conversion relationship, yielding a proportional constant called the enantiomeric enrichment factor, εer. in the present study we demonstrate a quantitative structure-activity relationship model (qsar) that describes well the dependence of εer on molecular structure. the enantiomeric enrichment factor can be predicted by the linear hansch model, whi ... | 2015 | 26153539 |
| chiral and isotope analyses for assessing the degradation of organic contaminants in the environment: rayleigh dependence. | the rayleigh equation is frequently used to describe isotope fractionation as a function of conversion. in this article we propose to draw a parallel between isotope and enantiomeric enrichments and derive a set of conditions that allow the use of the rayleigh approach to describe the enantiomeric enrichment-conversion dependencies. we demonstrate an implementation of the rayleigh equation for the enantioselective enzymatic hydrolysis of mecoprop-methyl, dichlorprop-methyl, and dimethyl-methylsu ... | 2014 | 24471759 |
| utilization of size polymorphism in its1 and its2 regions for identification of pathogenic yeast species. | despite the existence of a variety of available yeast-identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. the internal transcribed spacer (its) regions of fungal rrna genes exhibit variable sizes depending on the yeast species. in the present study, fragment size polymorphism (fsp) analysis of the its1 and its2 regions for identification of the clinically most important yeast species was assessed. | 2017 | 28260588 |
| utilization of size polymorphism in its1 and its2 regions for identification of pathogenic yeast species. | despite the existence of a variety of available yeast identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. the internal transcribed spacer (its) regions of fungal rrna genes exhibit variable sizes depending on the yeast species. in the present study, fragment size polymorphism (fsp) analysis of the its1 and its2 regions for identification of the clinically most important yeast species was assessed. the its1 and its2 regions of 1 ... | 2017 | 28068213 |
| an improved amperometric triglyceride biosensor based on co-immobilization of nanoparticles of lipase, glycerol kinase and glycerol 3-phosphate oxidase onto pencil graphite electrode. | nanoparticles (nps) of commercial lipase from candida rugosa, of glycerol kinase (gk) from cellulomonas species, of glycerol-3- phosphate oxidase (gpo) from aerococcus viridans were prepared, characterized and co-immobilized onto a pencil graphite (pg) electrode. the morphological and electrochemical characterization of pg electrode was performed by scanning electron microscopy (sem), cyclic voltammetry (cv) and electrochemical impedance spectroscopy (eis) before and after co-immobilization of e ... | 2017 | 28284306 |
| adhesion and biofilm formation in artificial saliva and susceptibility of yeasts isolated from chronic kidney patients undergoing haemodialysis. | yeasts of the genera candida and saccharomyces are opportunist pathogens and cause oral lesions, especially in immunocompromised patients. this study assessed yeasts isolated from chronic kidney patients undergoing haemodialysis for their adhesion capacity, biofilm formation and susceptibility to antifungal agents. ten isolates of candida spp. and one isolate of saccharomyces cerevisiae were tested for adhesion to buccal epithelial cells (becs), adhesion and formation of biofilm in artificial sa ... | 2015 | 26297016 |
| study of microwave effects on the lipase-catalyzed hydrolysis. | the effect of microwave heating on lipase-catalyzed reaction remains controversial. it is not clear whether the reaction rate enhancements are purely due to thermal/heating effects or to non-thermal effects. therefore, quantitative mass spectrometry was used to conduct accurate kinetic analysis of lipase-catalyzed hydrolysis of triolein by microwave and conventional heating. commercial lipases from candida rugosa (crl), porcine pancreas (ppl), and burkholderia cepacia (bcl) were used. hydrolysis ... | 2016 | 26672464 |
| candida/candida biofilms. first description of dual-species candida albicans/c. rugosa biofilm. | denture liners have physical properties that favour plaque accumulation and colonization by candida species, irritating oral tissues and causing denture stomatitis. to isolate and determine the incidence of oral candida species in dental prostheses, oral swabs were collected from the dental prostheses of 66 patients. all the strains were screened for their ability to form biofilms; both monospecies and dual-species combinations were tested. candida albicans (63 %) was the most frequently isolate ... | 2016 | 27020154 |
| molecular bioimprinting of lipases with surfactants and its functional consequences in low water media. | lipases from thermomyces lanuginosa (tll), candida rugosa (crl) and burkholderia cepacia (bcl) were obtained in the 'open lid' form by adding surfactant molecules like n-octyl-β-d-glucopyranoside (og), hexadecyl trimethyl ammonium bromide (ctab), bis(2-ethylhexyl) sulfosuccinate sodium salt (aot) and triton x-100 for this purpose. the enzymes were 'dried' by precipitating with 4× (v/v) excess of organic solvents. the imprint surfactant molecules were removed by extensive washing with organic sol ... | 2015 | 26306412 |
| chemical composition profiling and antifungal activity of the essential oil and plant extracts of mesembryanthemum edule (l.) bolus leaves. | essential oil from mesembryanthemum edule leaves have been used by the eastern cape traditional healers for the treatment of respiratory tract infections, tuberculosis, dysentery, diabetic mellitus, laryngitis and vaginal infections. the investigation of bioactive compounds in the essential oil of this plant could help to verify the efficacy of the plant in the management or treatment of these illnesses. | 2014 | 25392576 |
| candida albicans isolates from a malaysian hospital exhibit more potent phospholipase and haemolysin activities than non-albicans candida isolates. | this study was aimed at determining the phospholipase and haemolysin activity of candida isolates in malaysia. a total of 37 candida clinical isolates representing seven species, candida albicans (12), candida tropicalis (8), candida glabrata (4), candida parapsilosis (1), candida krusei (4), candida orthopsilosis (1) and candida rugosa (7) were tested. in vitro phospholipase activity was determined by using egg yolk plate assay whereas in vitro haemolysin activity was tested by using blood plat ... | 2013 | 24522136 |
| rapid identification of yeast isolates from clinical specimens in critically ill trauma icu patients. | the purpose was to evaluate the performance of a commercially available chromogenic candida speciation media and the vitek 2 id system for the identification of medically important yeasts and yeast-like organisms in a routine clinical microbiology laboratory. | 2012 | 22923919 |
| oral microbial colonization in children with sickle cell anaemia under long-term prophylaxis with penicillin. | sickle cell anaemia (sca) is the most frequent haematological hereditary disease. children with sca are submitted to long-term prophylactic therapy with penicillin, but little is known about its impact on oral microflora. the aim of this study was to evaluate the oral microbial colonization of paediatric patients with sca. | 2014 | 24967510 |
| diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |
| biodiversity and probiotic potential of yeasts isolated from fura, a west african spontaneously fermented cereal. | fura is a spontaneously fermented pearl millet product consumed in west africa. the yeast species involved in the fermentation were identified by pheno- and genotypic methods to be candida krusei, kluyveromyces marxianus, candida tropicalis, candida rugosa, candida fabianii, candida norvegensis and trichosporon asahii. c. krusei and k. marxianus were found to be the dominant species. survival in ph 2.5 or in the presence of bile salts (0.3% (w/v) oxgall) and growth at 37°c were independently det ... | 2012 | 23072700 |
| screening of invasive fungal infections by a real-time panfungal (pan-acf) polymerase chain reaction assay in patients with haematological malignancy. | invasive fungal infection (ifi) is a fatal infection in haematology patients. there is an urgent need for reliable screening methods facilitating timely diagnosis and treatment. a real-time panfungal polymerase chain reaction (pcr) assay based on taqman technology targeting 18s ribosomal rna gene was used to screen whole blood specimen obtained from series of haematology malignancy patients for ifis. | 2017 | 28303817 |
| investigation of deactivation thermodynamics of lipase immobilized on polymeric carrier. | in the present work, we have investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material. immobilization of lipase candida rugosa (crl) was carried out on biocompatible blend of poly vinyl alcohol (pva) and chitosan (chy) support via entrapment and glutardehyde (glu) cross-linking method to produce pva:chy:crl and pva:chy:glu:crl as robust biocatalyst. these immobilized lipases were characterized by various physico-biochemical characterization t ... | 2017 | 28265745 |
| regio-selective lipase catalyzed hydrolysis of oxanorbornane-based sugar-like amphiphiles at air-water interface: a polarized ft-irras study. | interfacial hydrolysis of oxanorbornane-based amphiphile (triol c16) by candida rugosa lipase was investigated using real-time polarized fourier transform-infrared reflection absorption spectroscopy (ft-irras). the kinetics of hydrolysis was studied by analyzing the ester carbonyl ν(co) stretching vibration band across the two dimensional (2d) array of molecules at the confined interface. in particular, we demonstrate triol c16 to form michaelis-menten type complex, like that of lipid-substrate ... | 2017 | 28235449 |
| co-immobilization of enzymes and magnetic nanoparticles by metal-nucleotide hydrogelnanofibers for improving stability and recycling. | in this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (mnps) using metal coordinated hydrogel nanofibers. candida rugosa lipase (crl) was selected as guest protein. for good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (ca-fe₃o₄ nps) have been widely used for immobilizing enzymes. as a result, the relative activity of ca-fe₃o₄@zn/amp nanofiber-immobilized crl increased by 8-fold ... | 2017 | 28125003 |
| evolutionary history of versatile-lipases from agaricales through reconstruction of ancestral structures. | fungal "versatile carboxylic ester hydrolases" are enzymes with great biotechnological interest. here we carried out a bioinformatic screening to find these proteins in genomes from agaricales, by means of searching for conserved motifs, sequence and phylogenetic analysis, and three-dimensional modeling. moreover, we reconstructed the molecular evolution of these enzymes along the time by inferring and analyzing the sequence of ancestral intermediate forms. | 2017 | 28049436 |
| development of a novel immobilization method by using microgels to keep enzyme in hydrated microenvironment in porous hydrophobic membranes. | using colloidal polyacrylamide (paam) microgels as carriers, a novel strategy for covalent immobilization of enzymes maintained in hydrated microenvironment on/in a macroporous surface-functionalized hydrophobic polyvinylidene fluoride (pvdf) membrane is developed. the paam microgels are synthesized by inverse miniemulsion polymerization, and first the parameters are investigated which are suited to obtain particles in the desired size range, 100-200 nm, with narrow size distribution. amino func ... | 2016 | 28026147 |
| collagen-immobilized lipases show good activity and reusability for butyl butyrate synthesis. | candida rugosa lipases were immobilized onto collagen fibers through glutaraldehyde cross-linking method. the immobilization process has been optimized. under the optimal immobilization conditions, the activity of the collagen-immobilized lipase reached 340 u/g. the activity was recovered of 28.3 % by immobilization. the operational stability of the obtained collagen-immobilized lipase for hydrolysis of olive oil emulsion was determined. the collagen-immobilized lipase showed good tolerance to t ... | 2016 | 27188972 |
| pcr-denaturing gradient gel electrophoresis analysis of microbial community in soy-daddawa, a nigerian fermented soybean (glycine max (l.) merr.) condiment. | soy-daddawa, a fermented soybean (glycine max (l.) merr.) condiment, plays a significant role in the culinary practice of west africa. it is essential to understand the microbial community of soy-daddawa for a successful starter culture application. this study investigated the microbial community structure of soy-daddawa samples collected from nigerian markets, by pcr-denaturing gradient gel electrophoresis (dgge) targeting the v3-v5 region of the 16s rrna gene of bacteria and internal transcrib ... | 2016 | 26796580 |
| determination of yeast diversity in ogi, mawè, gowé and tchoukoutou by using culture-dependent and -independent methods. | the maize based ogi and mawè and the sorghum based gowé and tchoukoutou are traditional, spontaneously fermented products widely consumed by the population of benin (west africa). yeast occurrence in the products, as sold on local markets at different locations, was studied using a combination of culture-dependent and independent methods. number of yeasts is varied from 3.75 log10 colony forming units (cfu)/g for ogi to 5.60 log10 cfu/g for tchoukoutou. isolated yeasts (236) were identified base ... | 2013 | 23727651 |
| role of plasmid in diesel oil degradation by yeast species isolated from petroleum hydrocarbon-contaminated soil. | five yeast species, namely candida tropicalis, cryptococcus laurentii, trichosporon asahii, rhodotorula mucilaginosa and candida rugosa isolated from hydrocarbon-contaminated soil were found to be potent degraders of diesel oil. these microorganisms showed the presence of enzymes cytochrome p450, nadph cytochrome c reductase, aminopyrine n demethylase, alcohol dehydrogenase, aldehyde dehydrogenase, naphthalene dioxygenase, catalase and glutathione s transferase when the cells were incubated for ... | 2012 | 22629639 |
| highly productive and enantioselective enzyme catalysis under continuous supported liquid-liquid conditions using a hybrid monolithic bioreactor. | enzyme-containing ionic liquids (ils) were immobilized in cellulose-2.5-acetate microbeads particles embedded in a porous monolithic polyurethane matrix. this bioreactor was used under continuous liquid-liquid conditions by dissolving the substrates in a nonpolar organic phase immiscible with the ils, thereby creating a biphasic system. lipases (candida antarctica lipase b, calb, candida rugosa lipase, crl) were used to catalyze the enantioselective transesterification of racemic (r,s)-1-phenyle ... | 2016 | 27650312 |
| ultrasonic enhancement of lipase-catalysed transesterification for biodiesel synthesis. | the production of biodiesel was carried out from canola oil and methanol catalysed by lipase from candida rugosa under different ultrasonic experimental conditions using horn (20khz) and plate (22, 44, 98 and 300khz) transducers. the effects of experimental conditions such as horn tip diameter, ultrasonic power, ultrasonic frequency and enzyme concentrations on biodiesel yield were investigated. the results showed that the application of ultrasound decreased the reaction time from 22-24h to 1.5h ... | 2017 | 27773250 |
| synthesis of medium-chain length capsinoids from coconut oil catalyzed by candida rugosa lipases. | a commercial preparation of candida rugosa lipases (crl) was tested for the production of capsinoids by esterification of vanillyl alcohol (va) with free fatty acids (fa) and coconut oil (co) as acyl donors. screening of fa chain length indicated that c8-c12 fa (the most common fa found in co triglycerides) are the best acyl-donors, yielding 80-85% of their specific capsinoids. hence, when co, which is rich in these fa, was used as the substrate, a mixture of capsinoids (vanillyl caprylate, vani ... | 2017 | 27719942 |
| physical and covalent immobilization of lipase onto amine groups bearing thiol-ene photocured coatings. | in this study, amine groups containing thiol-ene photocurable coating material for lipase immobilization were prepared. lipase (ec 3.1.1.3) from candida rugosa was immobilized onto the photocured coatings by physical adsorption and glutaraldehyde-activated covalent bonding methods, respectively. the catalytic efficiency of the immobilized and free enzymes was determined for the hydrolysis of p-nitrophenyl palmitate and also for the synthesis of p-nitrophenyl linoleate. the storage stability and ... | 2017 | 27704477 |
| a general and efficient strategy for generating the stable enzymes. | the local flexibility of an enzyme's active center plays pivotal roles in catalysis, however, little is known about how the flexibility of these flexible residues affects stability. in this study, we proposed an active center stabilization (acs) strategy to improve the kinetic thermostability of candida rugosa lipase1. based on the b-factor ranking at the region ~10 å within the catalytic ser209, 18 residues were selected for site-saturation mutagenesis. based on three-tier high-throughput scree ... | 2016 | 27667190 |
| deciphering the toxicity of bisphenol a to candida rugosa lipase through spectrophotometric methods. | bisphenol a is widely used in the manufacture of food packaging and beverage containers and can invade our food and cause contamination. candida rugose lipase has been a versatile enzyme for biocatalysis and biotransformations to produce useful materials for food, pharmaceutical and flavor. the interactions between bisphenol a and candida rugosa lipase in vitro were studied by uv-vis, steady-state fluorescence, circular dichroism, synchronous fluorescence, light scattering spectra, molecular doc ... | 2016 | 27529468 |
| efficacy of echinocandins against murine infections by diutina (candida) rugosa. | echinocandins are recommended as a first-line therapy for invasive candidiasis. candida rugosa was recently transferred to the new genus diutina. we have determined the in vitro killing kinetics of two echinocandins, anidulafungin, and caspofungin and their in vivo efficacy, administering doses of 5 or 10 mg/kg, and 1 or 5 mg/kg, respectively against 2 clinical strains of d. rugosa. both drugs showed a fungicidal concentration-dependent activity and, in a neutropenic murine model of disseminated ... | 2016 | 27342787 |
| collaborative study of an indirect enzymatic method for the simultaneous analysis of 3-mcpd, 2-mcpd, and glycidyl esters in edible oils. | a collaborative study was conducted to evaluate an indirect enzymatic method for the analysis of fatty acid esters of 3-monochloro-1,2-propanediol (3-mcpd), 2-monochloro-1,3-propanediol (2-mcpd), and glycidol (gly) in edible oils and fats. the method is characterized by the use of candida rugosa lipase, which hydrolyzes the esters at room temperature in 30 min. hydrolysis and bromination steps convert esters of 3-mcpd, 2-mcpd, and glycidol to free 3-mcpd, 2-mcpd, and 3-monobromo-1,2-propanediol, ... | 2016 | 27321120 |
| composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: magnetic material for bioapplications. | a simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. this ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, tem, sem), scattering (static and dynamic light scattering, sans) and spectroscopy (ftir) techniques. magnetic chitosan was found to be a perspec ... | 2016 | 27287146 |
| production of 3-hydroxypropionic acid via the propionyl-coa pathway using recombinant escherichia coli strains. | our study aimed to produce the commercially promising platform chemical 3-hydroxypropionic acid (3-hp) via the propionyl-coa pathway in genetically engineered escherichia coli. recombinant e. coli ec-p overexpressing propionyl-coa dehydrogenase (pacd, encoded by the pacd gene from candida rugosa) under the t7 promoter produced 1.33 mm of 3-hp in a shake flask culture supplemented with 0.5% propionate. when propionate coa-transferase (pct, encoded by the pct gene from megasphaera elsdenii) and 3- ... | 2016 | 27227837 |
| reversible immobilization of lipases on heterofunctional octyl-amino agarose beads prevents enzyme desorption. | two different heterofunctional octyl-amino supports have been prepared using ethylenediamine and hexylendiamine (oceda and ochda) and utilized to immobilize five lipases (lipases a (cala) and b (calb) from candida antarctica, lipases from thermomyces lanuginosus (tll), from rhizomucor miehei (rml) and from candida rugosa (crl) and the phospholipase lecitase ultra (lu). using ph 5 and 50 mm sodium acetate, the immobilizations proceeded via interfacial activation on the octyl layer, after some ion ... | 2016 | 27196882 |
| [structure and activity of fungal lipases in bile salt solutions]. | the changes in structure and catalytic properties of fungal lipases (candida rugosa, rhizomucor miehei, mucor javanicus) were investigated in micellar solutions of bile salts that differ in hydrophilic-lypophilic balance and reaction medium properties. the methods of circular dichroism and tryptophan fluorescence were applied to estimate the changes in peptide structure within complexes with bile salt micelles. bile salts do not exert a significant influence on the structure of the enzymes under ... | 2016 | 27192825 |
| structural traits and catalytic versatility of the lipases from the candida rugosa-like family: a review. | lipases and sterol esterases are enzymes with broad biotechnological applications, which catalyze the hydrolysis or synthesis of long-chain acylglycerols and sterol esters, respectively. in this paper, we review the current knowledge on the so-called candida rugosa-like family of enzymes, whose members display in most cases affinity against the two substrates mentioned above. the family includes proteins with the α/β-hydrolase folding, sharing conserved motifs in their sequences, and common stru ... | 2016 | 27188926 |
| optimization of the production and characterization of lipase from candida rugosa and geotrichum candidum in soybean molasses by submerged fermentation. | this present work describes the production and biochemical characterization of lipase by candida rugosa and geotrichum candidum in a culture supplemented with soybean molasses. after optimizing the fermentation times for both microorganisms, the effects of changing the soybean molasses concentration, the fermentative medium ph and the fermentation temperature were evaluated using the central composite planning. when soybean molasses was used at a concentration of 200 g/l at 27 ± 1 °c and ph 3.5, ... | 2016 | 27057641 |
| counteraction of trehalose on n, n-dimethylformamide-induced candida rugosa lipase denaturation: spectroscopic insight and molecular dynamic simulation. | candida rugosa lipase (crl) has been widely used as a biocatalyst for non-aqueous synthesis in biotechnological applications, which, however, often suffers significant loss of activity in organic solvent. experimental results show that trehalose could actively counteract the organic-solvent-induced protein denaturation, while the molecular mechanisms still don't unclear. herein, crl was used as a model enzyme to explore the effects of trehalose on the retention of enzymatic activity upon incubat ... | 2016 | 27031946 |
| influence of protein bulk properties on membrane surface coverage during immobilization. | biomolecules immobilization is a key factor for many biotechnological applications. for this purpose, the covalent immobilization of bovine serum albumin (bsa), lipase from candida rugosa and protein g on differently functionalized regenerated cellulose membranes was investigated. dynamic light scattering and electrophoresis measurements carried out on biomolecules in solution indicated the presence of monomers, dimers and trimers for both bsa and protein g, while large aggregates were observed ... | 2016 | 27022871 |
| co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection. | preserving enzyme activity and promoting synergistic activity via co-localization of multiple enzymes are key topics in bionanotechnology, materials science, and analytical chemistry. this study reports a facile method for co-immobilizing multiple enzymes in metal coordinated hydrogel nanofibers. specifically, four types of protein enzymes, including glucose oxidase, candida rugosa lipase, α-amylase, and horseradish peroxidase, were respectively encapsulated in a gel nanofiber made of zn(2+) and ... | 2016 | 26932320 |
| facilitation as attenuating of environmental stress among structured microbial populations. | there is currently an intense debate in microbial societies on whether evolution in complex communities is driven by competition or cooperation. since darwin, competition for scarce food resources has been considered the main ecological interaction shaping population dynamics and community structure both in vivo and in vitro. however, facilitation may be widespread across several animal and plant species. this could also be true in microbial strains growing under environmental stress. pure and m ... | 2016 | 26904719 |
| optimization of the hydrolysis of safflower oil for the production of linoleic acid, used as flavor precursor. | commercial lipases, from porcine pancreas (ppl), candida rugosa (crl), and thermomyces lanuginosus (lipozyme tl im), were investigated in terms of their efficiency for the hydrolysis of safflower oil (so) for the liberation of free linoleic acid (la), used as a flavor precursor. although ppl, under the optimized conditions, showed a high degree of hydrolysis (91.6%), its low tolerance towards higher substrate concentrations could limit its use for so hydrolysis. in comparison to the other invest ... | 2015 | 26904663 |
| design of biocompatible immobilized candida rugosa lipase with potential application in food industry. | biocatalysts are a promising alternative for the production of natural flavor compounds. candida rugosa lipase (crl) is a particularly important biocatalyst owing to its remarkable efficiency in both hydrolysis and synthesis. however, additional stabilization is necessary for successful industrial implementation. this study presents an easy and time-saving method for immobilizing this valuable enzyme on hydroxyapatite (hap), a biomaterial with high protein-binding capacity. | 2016 | 26801832 |
| optimization and modeling for the synthesis of sterol esters from deodorizer distillate by lipase-catalyzed esterification. | in this paper, cotton seed oil deodorizer distillate (csodd), was recovered to obtain fatty acid sterol ester (fase), which is one of the biological activated substances added as human therapeutic to lower cholesterol. esterification reactions were carried out using candida rugosa lipase (crl) as catalyst, and the conversion of phytosterol was optimized using response surface methodology (rsm). the highest conversion (90.8±0.4%) was reached at 0.84 wt% enzyme load, 1:25 solvent/csodd mass ratio, ... | 2016 | 26757412 |