| quantitative culture of rhodococcus equi from the feces of horse. | the selective isolation of r. equi in nanc medium was made by quantitative culture of the organism in the feces. r. equi was observed in the feces of all the mares and foals investigated. the mean viable count of r. equi organisms in 1 gram of feces was 8.42 x 10(2) in the mares and 7.57 x 10(2) in the foals, and its 95% confidence limits were in a range of 6.48 x 10(2) to 1.09 x 10(3) and 4.19 x 10(2) to 1.37 x 10(3), respectively. this result indicates that r. equi is a member of the normal in ... | 1983 | 6680772 |
| ecology of rhodococcus equi. | a selective broth enrichment technique was used to study the distribution of rhodococcus equi in soil and grazing animals. rhodococcus equi was isolated from 54% of soils examined and from the gut contents, rectal faeces and dung of all grazing herbivorous species examined. rhodococcus equi was not isolated from the faeces or dung of penned animals which did not have access to grazing. the isolation rate from dung was much higher than from other samples and this was found to be due to the abilit ... | 1984 | 6719819 |
| isolation and characterization of an intracellular serine protease from rhodococcus erythropolis. | | 1982 | 6758705 |
| [rhodococcus isolated from a gluteal abscess]. | the pathogenic role of the genus rhodococcus as an infectious agent in human beings is very small. its taxonomic allocation may be difficult since there is no systematic description of this bacterial group. this paper summarizes the international literature on the microbiologic diagnosis of rhodococcus and the small number of known cases of rhodococcus infections. a description is given of the first case of a post-injection gluteal abscess caused by a rhodococcus sp. | 1982 | 6759413 |
| numerical analysis of the taxonomy of nocardiae and rhodococci. | the genera nocardia and rhodococcus were clearly differentiated in the present study. eleven characteristics were shown to be useful for differentiation between these two genera. nocardia asteroides sunsu stricto previously defined by tsukamura was divided into two taxa. one contained the type strain and was considered to retain the name nocardia asteroides in a new sense. another was named in the present study as nocardia nova sp. nov. tsukamura. the type strain of this species is atcc 33726. t ... | 1982 | 6763136 |
| [development cycles of coryneform and nocardia-like bacteria]. | the growth cycles and the types of cell separation were studied in a microchamber with the collection strains of brevibacterium ammoniagenes attc 6871, b. helvolum atcc 19239, b. linens ccm 47 and atcc 9174, b. maris vkm b-464 and b. stationis atcc 14403, as well as with the strains of the genus rhodococcus isolated from soils, viz. r. maris sp. nov. imb 283 and r. luteus sp. nov. imb 385. according to the increasing complexity of cellular morphological transformation in the life cycle, the orga ... | 1980 | 6782438 |
| test for susceptibility to mitomycin c as aids for differentiating the genus rhodococcus from the genus nocardia, and for differentiating mycobacterium fortuitum and mycobacterium chelonei from other rapidly growing mycobacteria. | | 1981 | 6799752 |
| routine test for in vitro differentiation of pathogenic and apathogenic listeria monocytogenes strains. | the exosubstance of rhodococcus equi in a prepurified form strongly enhanced the hemolytic effect of certain strains of listeria monocytogenes. the strains which produced positive synergic hemolysis with this exosubstance were also pathogenic for guinea pigs and white mice. the other strains, which remained nonhemolytic in the presence of the r. equi. exosubstance, were apathogenic for those animals. a routine test was devised for the in vitro determination of the pathogenicity of l. monocytogen ... | 1982 | 6804488 |
| numerical classification of some rhodococci, corynebacteria and related organisms. | nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ... | 1982 | 6811696 |
| hemolytic properties of exosubstance of serovar 5 listeria monocytogenes compared with beta toxin of staphylococcus aureus. | hemolytic exosubstance of serovar 5 listeria monocytogenes, tested in native and pre-purified forms, produced identical interactions with exosubstances of certain bacterial species to those of staphylococcus aureus beta toxin on agars with ovine, rabbit, human and equine erythrocytes. in the same way like the staphylococcal beta toxin, it enhanced hemolysis of other l. monocytogenes serovars, though only slightly and after a longer time of incubation. with the hemolysin of corynebacterium pyogen ... | 1982 | 6812312 |
| fatty acid composition of some mycolic acid-containing coryneform bacteria. | the fatty acid profiles of 74 strains of mycolic acid-containing coryneform bacteria were examined by gas-liquid chromatography. all of the strains contained major amounts of straight-chain and monounsaturated fatty acids although some also possessed substantial amounts of 10-methyloctadecanoic acid. iso- and anteiso-branched acids were not present. five distinct fatty acid patterns were evident: (i) corynebacterium diphtheriae, c. pseudotuberculosis and 'c. ulcerans' strains contained major amo ... | 1982 | 6818327 |
| numerical classification of rhodococcus equi and related actinomycetes. | | 1982 | 6819287 |
| is rhodococcus equi a soil organism? | a total of 189 isolates of rhodococcus equi and related organisms and 16 marker strains representing the genera rhodococcus and corynebacterium were screened for 160 unit characters in a numerical taxonomic study. analysis of the data indicated that r. equi forms a relatively homogeneous cluster distinctly separated from the recognized species of rhodococcus and corynebacterium (sensu stricto). other members of the genus rhodococcus are soil organisms and r. equi appears to fit into the genus on ... | 1982 | 6962888 |
| immunity to and immunotherapy for rhodococcus equi. | immune responses to rhodococcus equi were assayed in mares and foals on 7 studs in south-eastern australia using skin test reactivity to the intradermal injection of culture filtrate and an indirect fluorescent antibody test. the prevalence of positive skin-test reactions did not differ between studs with a history of r. equi disease and those without but there were more mares with high antibody titres on studs with a disease history. a leucocyte extract prepared from mares that were skin-test p ... | 1982 | 6962890 |
| numerical classification of some named strains of nocardia asteroides and related isolates from soil. | one hundred and forty-nine strains of nocardiae, freshly isolated from soil samples obtained from a number of countries with either tropical or temperate climates, and from rubber pipe seals, were compared with appropriate marker cultures in a numerical phenetic study using 156 unit characters. marker strains were chosen to represent the nocardia asteroides complex, other nocardia species and related taxa in an effort both to classify the new soil isolates and, possibly, clarify the structure of ... | 1980 | 7003057 |
| comparison of three techniques for isolation of rhodococcus (corynebacterium) equi from contaminated sources. | inoculation of a liquid medium comprised of trypticase soy broth (bbl microbiology systems), cycloheximide, nalidixic acid, penicillin, and potassium tellurite and subcultured onto m3 medium plus potassium tellurite was highly successful for the isolation of rhodococcus (corynebacterium) equi from soil. | 1981 | 7007424 |
| the distribution of mycobacterium bovis in queensland cattle herds with observations on the laboratory diagnosis of tuberculosis. | of 1,791 tissues submitted from 1975 to 1978 for histological examination for tuberculosis, 78.5% were classified as tuberculosis, 8.1% as club-forming granulomas, 3.3% as hydatid cysts, 3.4% as other granulomas and 2.4% as squamous cell carcinomas. cultural examination of 2,682 tissues for mycobacteria yielded mycobacterium bovis from 42.88%, other mycobacteria from 7.64% and 0.89% rhodococcus (corynebacterium) equi. of 1,478 tissues on which both examinations were performed, culture and histol ... | 1980 | 7018487 |
| differentiation between the genera mycobacterium, rhodococcus and nocardia by susceptibility to 5-fluorouracil. | a test for susceptibility to 5-fluorouracil was useful for differentiating between species of rapidly growing mycobacteria, and for differentiating the genus rhodococcus from the genus nocardia. the majority of rhodococci tested were susceptible to 5-fluorouracil (20 micrograms ml-1), whereas the majority of nocardiae tested were resistant to it. strains of nocardia asteroides sensu stricto could be divided into two subgroups by their reaction to 5-fluorouracil. | 1981 | 7038035 |
| a survey of mycobacteriosis of feral pigs in the northern territory. | seven hundred and fifty-one feral pigs from the subcoastal plains of the northern territory were examined. the sample population consisted of 52.4% females and 47.6% males. they ranged in age from newborn piglets to mature animals of over 72 months. of the pigs examined 47.7% had macroscopic abscesses and of these 80.2% were probably caused by mycobacteria. tissues from 193 pigs were examined bacteriologically and 93 strains of mycobacteria were isolated. these were typed as m. bovis (37 strains ... | 1981 | 7041875 |
| capsular serotypes of rhodococcus equi. | one hundred strains of rhodococcus equi from various animal species and sources in australia were examined for capsular serotype. eighty-four of the strains fell into the existing 7 serotypes, and just under half of the strains belonged to serotype 1. isolates from the intestines and faeces of horses, cattle, pigs and other species, and from soil, were found to belong to the same serotypes as those recovered from the lungs of foals with r. equi pneumonia. there was no clear relationships between ... | 1982 | 7082241 |
| a survey of the structures of mycolic acids in corynebacterium and related taxa. | acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. the mycolic acids of corynebacterium sensu stricto strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of c22 to c36. two species, corynebacterium bovis and 'corynebacterium mycetoides', were particularly distinctive; the former had exceptionally low molecular weight mycolic a ... | 1982 | 7086391 |
| [key rhodococcus enzymes in the catabolism of aromatic compounds]. | the enzyme apparatus involved in the catabolism of aromatic compounds in rhodococci is characterized by the presence of pyrocatechase and protocatechoate-3,4-dioxygenase as principal enzymes cleaving the aromatic cycle. metapyrocatechase was found in about 30% of the rhodococci. all the enzymes are inducible. the inductor of pyrocatechase seems to be cyc-cys-muconate, and that of protocatechase appears to be 3-oxoadipate. the metapyrocatechase of rhodococci, in contrast to that of pseudomonas, i ... | 1982 | 7087811 |
| numerical and chemical classification of nocardia amarae. | twenty-one strains of nocardia amarae and marker cultures of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were subjected to numerical phenetic analyses using 92 unit characters. the data were examined using the simple matching (ssm), jaccard (sj) and pattern (dp) coefficients and clustering was achieved using the unweighted average linkage algorithm. neither cluster nor aggregate cluster composition was markedly affected by the coefficient used or by test error, estimated at 1 ... | 1982 | 7119738 |
| lung infection caused by gordona aurantiaca (rhodococcus aurantiacus). | we report that gordona aurantiaca (rhodococcus aurantiacus) caused a lung infection in humans. the case of a 50-year-old male farmer is shown. this is the first report of infection by this organism. | 1982 | 7153308 |
| in-vitro susceptibility of nocardia and rhodococcus to fourteen beta-lactam antibiotics. | | 1982 | 7161233 |
| the treatment of pneumonia in foals caused by rhodococcus (corynebacterium) equi. | | 1981 | 7259664 |
| rhodococcus (corynebacterium) equi: a possible cause of reactions to the complement fixation test for johne's disease of cattle. | | 1981 | 7271613 |
| porcine abortions associated with fungi, actinomycetes, and rhodococcus sp. | history, lesions, and results of microbiologic examinations are given for four porcine abortions associated with fungi and one each associated with actinomadura madurae, an aerobic actinomycete, and rhodococcus sp. the fungi were gliomastix sp., petriellidium boydii, aspergillus fumigatus, and exophiala jeanselmei. parvovirus, isolated from one fetus, was the only other infectious agent identified. the presence of fungi, actinomadura madurae, and rhodococcus sp. in fetal and placental lesions in ... | 1981 | 7281459 |
| [thin-layer chromatography after incubation with 35s-methionine.--meaning of petroleum ether-soluble fraction as aid to identification of mycobacterium, rhodococcus, and nocardia--(author's transl)]. | | 1980 | 7289348 |
| occurrence of rhodococcus coprophilus and associated actinomycetes in feces, sewage, and freshwater. | freshwater, sewage, and fecal samples from various sources were examined for rhodococcus coprophilus, associated actinomycetes, escherichia coli, and fecal streptococci. rhodococcus coprophilus was isolated consistently from feces of farm animals, poultry reared in proximity to farm animals, freshwater, and wastewater polluted with animal fecal wastes. it was not isolated from samples of human feces. the ratio of r. coprophilus total actinomycetes was higher in feces from cattle, sheep, ducks, a ... | 1981 | 7316510 |
| [difference of uptake of 2,6-diamino (g-3h) pimelic acid dihydrochloride among mycobacterium, rhodococcus, and nocardia (author's transl)]. | | 1980 | 7373966 |
| response of developing branched bacteria to adverse environments. ii. micromorphological effects of lysozyme on some aerobic actinomycetes. | an early symptom of lysozyme treatment of developing actinomycete microcolonies is hyphal tip swelling, illustrating the plasticity of this region in relation to filament extension. the reaction is not limited to true mycelia of streptomyces, streptoverticillium and rhodococcus but is also characteristic of unbranched filaments of actinomycetes exposed during their elongation stage. on the other hand rods whose extension has ceased and which are undergoing fragmentation do not show any localized ... | 1980 | 7424249 |
| septic arthritis and osteomyelitis caused by an organism of the genus rhodococcus. | we describe a previously healthy, immunologically normal young girl who presented painless swelling of fingers, a toe, and one knee. roentgenograms were consistent with osteomyelitis of the phalanges and knee effusion. rice bodies (corpora oryzoidea) were identified in viscous fluid obtained from the knee during arthroscopy. culture of this fluid grew an organism initially believed to be a member of the genus nocardia but which was later presumptively identified as a member of the genus rhodococ ... | 1981 | 7462414 |
| reclassification of mycobacterium phlei pn-bb as rhodococcus bronchialis pn-bb. | mycobacterium phlei pn-bb, induced in the parental strain pn by gamma-radiation and ethyl methanesulfonate, should be reclassified as rhodococcus bronchialis pn-bb. the reclassification is based on the results of lipid analysis, dna-dna hybridization and several biochemical tests, performed in comparison with the parental strain pn, earlier reclassified as r. bronchialis, and r, bronchialis (n654). | 1980 | 7469254 |
| rhodococcus equi-associated necrotizing lymphadenitis in a llama. | a case of rhodococcus equi-associated necrotizing lymphadenitis in a 2-year-old male llama is described. caseous necrosis, resembling macroscopically that seen in ovine caseous lymphadenitis, was observed diffusely in the tracheobronchial and mediastinal lymph nodes, and in an extensive lesion in the lungs. necrosis was present to a lesser extent in the spleen and hepatic and gastric lymph nodes. numerous bacteria-laden macrophages were present around the necrotic areas. the findings suggest tha ... | 1995 | 7490342 |
| rhodococcus equi pneumonia and pulmonary malakoplakia in acquired immunodeficiency syndrome. pathologic features. | pulmonary infection with rhodococcus equi is rare, and most cases are seen in immunocompromised patients, particularly those with acquired immunodeficiency syndrome. we describe the pathologic features in four cases of culture-positive r equi pneumonia occurring in patients infected with human immunodeficiency virus. all four patients had a solitary cavitary pulmonary mass that was resected (n = 3) or had undergone biopsy (n = 1). pathologically, all specimens revealed sheets of histiocytes with ... | 1994 | 7517660 |
| phylogenetic evidence for transfer of pentachlorophenol-mineralizing rhodococcus chlorophenolicus pcp-i(t) to the genus mycobacterium. | we determined the nucleotide sequence of a 16s rrna gene of rhodococcus chlorophenolicus pcp-i(t) (= dsm 43826t) (t = type strain). sequence comparisons revealed that there was a close relationship between strain pcp-i(t) and strains belonging to the genus mycobacterium. the sequence data were used to construct a phylogenetic tree, which showed that mycobacterium chubuense is the closest relative of strain pcp-i(t). we propose that strain pcp-i(t) should be transferred to the genus mycobacterium ... | 1994 | 7520738 |
| a phylogenetic analysis of the genus nocardia with 16s rrna gene sequences. | partial sequences of the 16s rrna genes of the type strains of nine species of the genus nocardia were determined following the isolation and cloning of the amplified genes. these sequences were aligned with the sequences of representatives of the genera corynebacterium, gordona, mycobacterium, rhodococcus, and tsukamurella, and phylogenetic trees were inferred by using the fitch-margoliash and neighbor-joining methods. the genus nocardia formed a distinct clade that was most closely associated ... | 1995 | 7537058 |
| a phylogeny of the genus nocardia deduced from the analysis of small-subunit ribosomal dna sequences, including transfer of nocardia amarae to the genus gordona as gordona amarae comb. nov. | according to phylogenetic analyses of nearly complete small-subunit ribosomal dna sequences, the genus nocardia should not comprise the two species nocardia petroleophila and nocardia amarae. n. amarae should be reassigned to the genus gordona as gordona amarae. all of the other nocardia species form a monophyletic unit, closely related to species of the genus rhodococcus. it is proposed to revive the name 'cmn' to comprise the genera corynebacterium, tsukamurella, mycobacterium, gordona, rhodoc ... | 1994 | 7545965 |
| phylogenetic analysis of mycolic acid-containing wall-chemotype iv actinomycetes and allied taxa by partial sequencing of ribosomal protein at-l30. | the phylogenetic relationships among 30 mycolic acid-containing wall chemotype iv actinomycete strains and 12 strains belonging to allied taxa were examined by determining the amino acid sequences of the ribosomal at-l30 proteins of these organisms. sequencing 20 n-terminal amino acids of at-l30 preparations revealed that the members of the genera containing mycolic acid-containing actinomycetes form two clusters; the first cluster contains the genera nocardia, rhodococcus, gordona, and tsukamur ... | 1995 | 7547284 |
| phylogenetic analysis of the genus corynebacterium based on 16s rrna gene sequences. | the 16s rrna gene sequences of 30 strains representing 23 validated corynebacterium species and 7 currently non-valid corynebacterium species were determined. these sequences were aligned with the sequences of other corynebacterium species and related actinomycete taxa. a comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus corynebacterium. turicella otitidis and the amycolate species corynebacterium amycolatum were located at t ... | 1995 | 7547291 |
| phylogeny of the genus corynebacterium deduced from analyses of small-subunit ribosomal dna sequences. | we determined almost complete small-subunit ribosomal dna sequences of 50 reference strains belonging to the genera corynebacterium, rhodococcus, and gordona and compared these sequences with previously published sequences. three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. the results of comparative phylogenetic analyses confirmed that the ge ... | 1995 | 7547293 |
| comparison of mycobacterium 23s rrna sequences by high-temperature reverse transcription and pcr. | we describe a modified rrna sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus mycobacterium. we combined the sensitivity of the reverse transcriptase pcr for amplifying nanogram amounts of template rrna material with the elevated extension temperatures used for the thermostable dna polymerase from thermus thermophilus. a 70 degrees c reverse transcription extension step permitted improved read-through of highly structured r ... | 1995 | 7547304 |
| assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16s rrna genes of corynebacteria. | comparative 16s rrna gene sequencing was used to assign four isolates of spontaneous urinary calculus in young laboratory rats. the phylogenetic relationships among the rat isolates and selected species of corynebacteria were also inferred. based on the homology and evolutionary distance analysis, the 16s rrna genes of the rat isolates were almost identical with that of corynebacterium renale atcc 19412. also the results of the phylogenetic analysis showed a close relationship among the isolates ... | 1995 | 7548409 |
| quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from comamonas testosteroni 63. the first two enzymes in quinoline and 3-methylquinoline degradation. | the enzymes catalysing the first two steps of quinoline and 3-methylquinoline degradation by comamonas testosteroni 63 were investigated. quinoline 2-oxidoreductase, which catalyses the hydroxylation of (3-methyl-)quinoline to (3-methyl-)2-oxo-1,2-dihydroquinoline, was purified to apparent homogeneity. the native enzyme, with a molecular mass of 360 kda, is composed of three non-identical subunits (87, 32, and 22 kda), occurring in a ratio of 1.16:1:0.83. containing fad, molybdenum, iron, and ac ... | 1995 | 7556204 |
| activation of protein kinase c by mycobacterial cord factor, trehalose 6-monomycolate, resulting in tumor necrosis factor-alpha release in mouse lung tissues. | cord factors are mycoloyl glycolipids in cell walls of bacteria belonging to actinomycetales, such as mycobacterium, nocardia and rhodococcus. they induce granuloma formation in the lung and interstitial pneumonitis, associated with production of macrophage-derived cytokines. we studied how cord factors induce biological activities in the cells. cord factors isolated from m. tuberculosis, trehalose 6-monomycolate (mtmm) and trehalose 6,6'-dimycolate (mtdm), enhanced protein kinase c (pkc) activa ... | 1995 | 7559098 |
| plasmid prtl1 controlling 1-chloroalkane degradation by rhodococcus rhodochrous ncimb13064. | rhodococcus rhodochrous ncimb13064 can dehalogenate and use a wide range of 1-haloalkanes as sole carbon and energy source. the 1-chloroalkane degradation phenotype may be lost by cells spontaneously or after treatment with mitomycin c. two laboratory derivatives of the original strain exhibited differing degrees of stability of the chloroalkane degradation marker. plasmids of approximately 100 kbp (prtl1) and 80 kbp (prtl2) have been found in r. rhodochrous ncimb13064. prtl1 was shown to be car ... | 1995 | 7568468 |
| the sulphydryl-activated cytolysin and a sphingomyelinase c are the major membrane-damaging factors involved in cooperative (camp-like) haemolysis of listeria spp. | the negative mutant approach was used in this study to identify listerial cytolytic factors involved in cooperative haemolysis (camp-like phenomenon) with staphylococcus aureus and rhodococcus equi. a listeria monocytogenes non-haemolytic mutant specifically impaired in listeriolysin o (llo) production gave no camp reaction with s. aureus, and was virtually camp-negative with r. equi, indicating that the listerial sulphydryl-activated toxin played a major role in cooperative haemolysis. this was ... | 1995 | 7569324 |
| sequence and molecular characterization of a dna region encoding the dibenzothiophene desulfurization operon of rhodococcus sp. strain igts8. | dibenzothiophene (dbt), a model compound for sulfur-containing organic molecules found in fossil fuels, can be desulfurized to 2-hydroxybiphenyl (2-hbp) by rhodococcus sp. strain igts8. complementation of a desulfurization (dsz) mutant provided the genes from rhodococcus sp. strain igts8 responsible for desulfurization. a 6.7-kb taqi fragment cloned in escherichia coli-rhodococcus shuttle vector prr-6 was found to both complement this mutation and confer desulfurization to rhodococcus fascians, ... | 1995 | 7574582 |
| multiple genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase in the gram-positive polychlorinated biphenyl-degrading bacterium rhodococcus erythropolis ta421, isolated from a termite ecosystem. | rhodococcus erythropolis ta421 was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (pcb) congeners. genetic and biochemical analyses of the pcb catabolic pathway of this organism revealed that there are four different bphc genes (bphc1, bphc2, bphc3, and bphc4) which encode 2,3-dihydroxybiphenyl dioxygenases. as determined by southern hybridization, none of the bphc genes exhibits homology to any other bphc gene. bphc1, bphc2, and bphc4 encode en ... | 1995 | 7574595 |
| characterization of the rhodococcus sp. ni86/21 gene encoding alcohol: n,n'-dimethyl-4-nitrosoaniline oxidoreductase inducible by atrazine and thiocarbamate herbicides. | a protein with a mol.mass of 51,000 (thce) that was induced in rhodococcus sp. ni86/21 during assimilation of thiocarbamate herbicides, atrazine, ethanol, propanol, glycerol, propionaldehyde or ethanolamine was identified by two-dimensional electrophoresis. the thce gene was cloned and sequenced. the deduced amino acid sequence revealed thce as a member of group iii alcohol dehydrogenases. thce displayed strong homology with sequenced subunit fragments of the homodecameric n,n'-dimethyl-4-nitros ... | 1995 | 7575099 |
| an improved escherichia coli-rhodococcus shuttle vector and plasmid transformation in rhodococcus spp. using electroporation. | the genetic studies of metabolically diverse rhodococcus spp. have been hampered by the lack of a system of introducing exogenous dna. the authors improved an existing escherichia coli-rhodococcus shuttle vector (pmvs301) by removing much of the dna not needed for replication and adding a multicloning site. this improved vector (pbs305) is 7.9 kb in length. its ability to transform rhodococcus was tested using electroporation parameters optimized for introduction of pmvs301 into rhodococcus. tra ... | 1995 | 7576519 |
| dehalogenation of haloalkanes by rhodococcus erythropolis y2. the presence of an oxygenase-type dehalogenase activity complements that of an halidohydrolase activity. | rhodococcus erythropolis y2 produced two types of dehalogenase: a hydrolytic enzyme, that is an halidohydrolase, which was induced by c3 to c6 1-haloalkane substrates, and at least one oxygenase-type dehalogenase induced by c7 to c16 1-haloalkanes and n-alkanes. the oxygenase-type activity dehalogenated c4 to c18 1-chloroalkanes with an optimum activity towards 1-chlorotetradecane. the halidohydrolase catalysed the dehalogenation of a wide range of 1- and alpha,omega-disubstituted haloalkanes an ... | 1995 | 7579998 |
| pathogenesis of rhodococcus equi infection in mice: roles of virulence plasmids and granulomagenic activity of bacteria. | virulence of rhocococcus equi atcc 33701 and its plasmid-cured derivative atcc 33701p- was compared in balb/c and c3h/hej mice in terms of bacterial growth kinetics and histological changes in the liver, spleen and lungs, and humoral immune responses. injection with a sublethal dose of 10(6) atcc 33701 in mice resulted in microabscess formation after rapid multiplication in the liver and spleen by day 4, and then the bacteria were gradually eliminated with the formation of granuloma and the prod ... | 1995 | 7581269 |
| the first characterization of a eubacterial proteasome: the 20s complex of rhodococcus. | the 26s proteasome is the central protease of the ubiquitin-dependent pathway of protein degradation. the proteolytic core of the complex is formed by the 20s proteasome, a cylinder-shaped particle that in archaebacteria contains two different subunits (alpha and beta) and in eukaryotes contains fourteen different subunits (seven of the alpha-type and seven of the beta-type). | 1995 | 7583123 |
| antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens). | the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ... | 1995 | 7587049 |
| sequence and expression of the bpdc1c2bade genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by rhodococcus sp. m5. | the nucleotide (nt) sequence of the bpdc1c2bade genes which encode the first three enzymes in the biphenyl (bp) degradation pathway of gram+ rhodococcus sp. m5 (formerly arthrobacter m5) was determined. except for the ferredoxin component (bpdb) of the initial bp dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (todc1c2bade) present in the toluene-degrader, pseudomonas putida f1, than those of three bp ... | 1995 | 7590299 |
| cloning and expression of the s-triazine hydrolase gene (trza) from rhodococcus corallinus and development of rhodococcus recombinant strains capable of dealkylating and dechlorinating the herbicide atrazine. | we used degenerate oligodeoxyribonucleotides derived from the n-terminal sequence of the s-triazine hydrolase from rhodococcus corallinus nrrl b-15444r in an amplification reaction to isolate a dna segment containing a 57-bp fragment from the trza gene. by using the nucleotide sequence of this fragment, a nondegenerate oligodeoxyribonucleotide was synthesized and used to screen a genomic library of r. corallinus dna for fragments containing trza. a 5.3-kb psti fragment containing trza was cloned ... | 1995 | 7592318 |
| identification of virulence-associated antigens and plasmids in rhodococcus equi from patients with aids. | rhodococcus equi is an emerging opportunistic pathogen of human immunodeficiency virus-infected patients. however, little is known about the characteristics of r. equi isolates from humans. this study characterized the plasmid content, expression of a virulence-associated antigen, and mouse virulence of 19 r. equi isolates from patients with and without aids. ecori digestion patterns and southern, western, and virulence analyses of these isolates with cryptic plasmids allowed definition of a new ... | 1995 | 7594668 |
| case reports: pericarditis and lymphadenitis due to rhodococcus equi. | most patients with rhodococcus equi infection are immunocompromised by either hiv infection, malignancy, or medication. diagnosis is frequently missed or delayed because the organisms, resembling diphtheroids on smears, may be regarded as contaminants. their clinical, pathologic, histochemical, and microbiologic resemblance to mycobacteria can result in misdiagnosis. two cases were seen recently in our institution. r. equi pericarditis developed in a 29-year-old woman with failed renal transplan ... | 1995 | 7604837 |
| pseudomonas aeruginosa aliphatic amidase is related to the nitrilase/cyanide hydratase enzyme family and cys166 is predicted to be the active site nucleophile of the catalytic mechanism. | a database search indicated homology between some members of the nitrilase/cyanide hydratase family, pseudomonas aeruginosa and rhodococcus erythropolis amidases and several other proteins, some of unknown function. block and profile searches confirmed these relationships and showed that four regions of the p. aeruginosa amidase had significant homology with corresponding regions of nitrilases. a phylogenetic tree placed the p. aeruginosa and r. erythropolis amidases in a group with nitrilases b ... | 1995 | 7607322 |
| cytokine modulation alters pulmonary clearance of rhodococcus equi and development of granulomatous pneumonia. | rhodococcus equi, a facultative intracellular bacterium, causes chronic, often fatal granulomatous pneumonia in young horses and in humans with aids. the inability of host alveolar macrophages to kill intracellular r. equi results in the development of granulomas and progressive loss of pulmonary parenchyma. clearance of the organism from the lung requires functional cd4+ t cells. the purpose of this study was to identify the cytokine effector mechanisms that mediate clearance of r. equi from th ... | 1995 | 7622227 |
| [pneumonia and bone marrow infection with rhodococcus equi in an aids patient]. | | 1995 | 7623501 |
| [susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine and combinations of these compounds]. | using a broth microdilution technique, the in vitro susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine, and combinations of these compounds was determined. the bacterial strains (n = 88) isolated recently from horses with respiratory symptoms belonged to the following species: streptococcus equi subsp. zooepidemicus (n = 34), streptococcus equi subsp. equi (n = 22), staphylococcus aureus (n = 9), klebsiella pneumoniae (n = 7), rh ... | 1995 | 7624856 |
| another cause of endobronchial lesions found in hiv patients. | | 1995 | 7634912 |
| characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (pcb)-degrading strains of comamonas testosteroni isolated from pcb-contaminated soil. | in this study, we isolated and characterized biphenyl (bp) and polychlorinated biphenyl (pcb) degrading bacterial strains found in pcb-contaminated soil from an auto manufacturing plant located in syracuse, new york. twenty-one bp and pcb-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. of the 21 bacteria, 13 were identified as comamonas testosteroni, constituting about 60% of the bacterial population examined. other pcb d ... | 1995 | 7641143 |
| aliphatic nitrilase from a soil-isolated comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure. | an aliphatic nitrilase, active on adiponitrile and cyanovaleric acid, was identified and purified from comamonas testosteroni sp. (ct). oligodeoxyribonucleotide probes were designed from limited amino acid (aa) sequence information and used to clone the corresponding gene, named nita. high homologies were found at the aa level between ct nitrilase and the sequences of known nitrilases. multi-alignment of sequenced nitrilases suggests that cys163 of ct plays an essential role in the active site. ... | 1995 | 7642130 |
| identification and activity of two insertion sequence elements in rhodococcus sp. strain igts8. | two putative insertion sequence (is) elements, is1166 and is1295, were identified on a plasmid present in rhodococcus sp. igts8. four copies of is1166 were present in strain igts8: one copy on each of two separate plasmids and two copies on a third plasmid or in the chromosome. of eight rhodococci tested, only r. zopfii contained a copy of an is1166-like element. two mutants of strain igts8 were isolated in which an additional copy of is1166 was present, suggesting that at least one copy of this ... | 1995 | 7642133 |
| identification of two new genes in the pseudomonas aeruginosa amidase operon, encoding an atpase (amib) and a putative integral membrane protein (amis). | the nucleotide sequence of the amidase operon of pseudomonas aeruginosa has been completed and two new genes identified amib and amis. the complete gene order for the operon is thus amiebcrs. the amib gene encodes a 42-kda protein containing an atp binding motif that shares extensive homology with the clp family of proteins and also to an open reading frame adjacent to the amidase gene from rhodococcus erythropolis. deletion of the amib gene has no apparent effect on inducible amidase expression ... | 1995 | 7642533 |
| a single cytochrome p-450 system is involved in degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine by rhodococcus sp. strain ni86/21. | during atrazine degradation by rhodococcus sp. strain n186/21, n-dealkylated metabolites and an hydroxyisopropyl derivative are produced. the cytochrome p-450 system that is involved in degradation of thiocarbamate herbicides by strain n186/21 (i. nagy, g. schoofs, f. compernolle, p. proost, j. vanderleyden, and r. de mot, j. bacteriol. 177:676-687, 1995) is also required for atrazine degradation. atrazine-degrading activity was conferred on the atrazine-negative strains, mutant faj2027 of rhodo ... | 1995 | 7646049 |
| cloning of the genes for degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine from rhodococcus sp. strain te1. | the degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in rhodococcus sp. strain te1. plasmid dna libraries of rhodococcus sp. strain te1 were constructed in a rhodococcus-escherichia coli shuttle vector, pbs305, and transferred into rhodococcus sp. strain te3, a derivative of rhodococcus sp. strain te1 lacking herbicide degradation activity, to select transformants capa ... | 1995 | 7646050 |
| identification of virulent rhodococcus equi by amplification of gene coding for 15- to 17-kilodalton antigens. | during a survey of the prevalence of virulent rhodococcus equi at horse-breeding farms by plasmid and protein profiles, cryptic plasmids of various sizes were found in 66 (3.8%) of 1,725 isolates from feces of horses and 129 (5.9%) of 2,200 isolates from soil. twenty-two isolates, which contained cryptic plasmids of different sizes, were found by plasmid profiles, and their protein profiles and mouse pathogenicities were examined. of the 22 isolates, 7 were virulent r. equi, contained both virul ... | 1995 | 7650199 |
| development of a new selective plating medium for rhodococcus equi. | a new selective plating medium for rhodococcus equi containing ceftazidime (20 mg/l) and novobiocin (25 mg/l) on a mueller-hinton agar basis is described. it proved to be less inhibitory for r. equi than selective plating media devised earlier and grew only very few other nocardioform bacteria. | 1995 | 7651242 |
| machine operator's lung. a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols. | six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. two workers also reported fever and weight loss. all six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. chest radiographs revealed pulmonary interstitial infiltrates in four workers. lung function tests showed that four workers had decreased diffusing capacity. after removal ... | 1995 | 7656609 |
| clinical pharmacologic aspects of cefixime in dogs. | the minimal inhibitory concentration (mic) of cefixime, a new third-generation orally administered caphalosporin, was determined for reference and clinical isolates from dogs. the mic of the drug for all but 1 of the 18 enterobacteriaceae isolates tested, 1 pasteurella canis, 1 rhodococcus equi, 1 streptococcus canis, and 1 streptococcus group g isolate, was less than 1.0 microgram/ml. the mic for 9 staphylococcus intermedius isolates ranged from 1.56 to 6.25 micrograms/ml and, for 8 sta aureus ... | 1995 | 7661460 |
| bacterial steroid monooxygenase catalyzing the baeyer-villiger oxidation of c21-ketosteroids from rhodococcus rhodochrous: the isolation and characterization. | steroid monooxygenase from rhodococcus rhodochrous, isolated in homogeneity with a high yield, catalyzes baeyer-villiger oxidation of progesterone to produce testosterone acetate with the stoichiometric consumptions of nadph and molecular oxygen. it is a flavoenzyme with the molecular size of 60 kda in the monomeric form and the isoelectric point of 4.9. the absorption spectrum has the maxima at 278, 376, and 439 nm and the shoulders at 360 and 465 nm, indicating a strong hypsochromic shift (blu ... | 1995 | 7669800 |
| infections due to rhodococcus equi in three hiv-infected patients: microbiological findings and antibiotic susceptibility. | infections of rhodococcus equi, a well-known pathogen in animals which causes cavitated pneumonia similar to that caused by mycobacteria, were studied in three hiv-infected patients. this microorganism was isolated in the bronchoalveolar washings of two patients and in the sputum of the third. in two patients, rh. equi represented the first clinical opportunistic manifestation of hiv disease. one patient died of concomitant pneumocystis infection. the eradication of the microorganism occurred in ... | 1994 | 7672872 |
| cloning, sequence analysis, and expression of the flavobacterium pentachlorophenol-4-monooxygenase gene in escherichia coli. | the pcpb gene of flavobacterium sp. strain atcc 39723 was cloned by using a degenerate primer designed from the n-terminal sequence of the purified enzyme. the nucleotide sequence of pcpb was determined and found to encode an open reading frame of 1,614 nucleotides, yielding a predicted translation product of 538 amino acids, in agreement with the estimated size of the purified protein analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the transcriptional start of pcpb was fo ... | 1993 | 7678243 |
| rhodococcus species fatal infection in an immunocompetent host. | a 24-year-old woman had fatal pneumonia-associated adult respiratory distress syndrome caused by rhodococcus species. histological examination of lung biopsy tissue showed intracellular coccobacillary microorganisms. antimicrobial susceptibility tests on the patient's blood isolate showed that it was resistant to clindamycin and norfloxacin but susceptible to several other antimicrobial agents. also, the isolate's biochemical reactions and pattern of rna gene-containing restriction fragments wer ... | 1993 | 7683869 |
| sequence of the rhodococcus equi gene encoding the virulence-associated 15-17-kda antigens. | the nucleotide sequence of the rhodococcus equi gene encoding the virulence-associated 15-17-kda antigens, located on plasmid preat701, has been determined. the gene encodes a 19-kda protein of 189 amino acids, with an ala-rich leader signal sequence (ss). at least five ss peptidase cleavage sites were found in this region. the molecular diversity of 15-17-kda antigens might be attributed to the multiple ss peptidase cleavage sites. | 1995 | 7698659 |
| establishment of oil-degrading bacteria associated with cyanobacteria in oil-polluted soil. | a unique natural microbial cocktail with promising potential for remediating oil-polluted desert in the gulf region is reported. oil-degrading micro-organisms immobilized within dense cyanobacterial mats on oily coasts of the arabian gulf were successfully established in oil-contaminated sand. those micro-organisms biodegraded 50% of the oil within 10-20 weeks. nocardioforms belonging to the genus rhodococcus predominated in the first few weeks, but after 22 weeks pseudomonas spp. increased, sha ... | 1995 | 7698954 |
| molecular characterization of a lipid-modified virulence-associated protein of rhodococcus equi and its potential in protective immunity. | virulent strains of rhodococcus equi produce plasmid-mediated 15- and 17-kda proteins, which are thermoregulated and apparently surface-expressed. we demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) that r. equi produce three antigenically-related virulence-associated proteins, a diffuse 18-22-kda, a 17.5-kda and a 15-kda protein. phase partitioning of whole cells of r. equi strain 103 with triton x-114 (tx-114) and labelling with [3h]-labelled palmitic acid s ... | 1995 | 7704843 |
| alpha-mannosidase: a rapid test for identification of arcanobacterium haemolyticum. | a 4-h alpha-mannosidase test for identification of arcanobacterium haemolyticum strains (n = 139) and differentiation of a. haemolyticum from actinomyces pyogenes strains (n = 30) and other gram-positive rods was evaluated. practically all a. haemolyticum strains (138 of 139) and the listeria monocytogenes type strain were alpha-mannosidase positive, while all a. pyogenes strains and corynebacterium (n = .25) strains as well as the rhodococcus equi and erysipelothrix rhusiopathiae type strains w ... | 1994 | 7710479 |
| rhodococcus meningitis in an immunocompetent host. | rhodococcus species are increasingly being recognized as pathogens, especially in patients infected with the human immunodeficiency virus. most cases of rhodococcus infection in these patients are due to rhodococcus equi and involve the lungs. cns infections due to rhodococcus species are rare, and meningitis due to non-equi rhodococcus has never been reported in a healthy host. we report a case of meningitis due to non-equi rhodococcus in a previously healthy 24-year-old woman. we also review a ... | 1995 | 7727645 |
| analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in alcaligenes eutrophus. | a 5.0-kbp genomic ecori restriction fragment which complemented a third subclass of polyhydroxyalkanoic acid (pha)-leaky mutants of a. eutrophus that accumulated pha at a lower rate than the wild type was cloned from alcaligenes eutrophus h16. a 687-bp phapae gene on this fragment encoded a 24-kda protein (m(r) = 23,963), which was referred to as the ga24 protein. the ga24 protein was solubilized from the granules and purified to electrophoretic homogeneity, and antibodies against the ga24 prote ... | 1995 | 7730274 |
| identification of the region of a 14-kilodalton protein of rhodococcus ruber that is responsible for the binding of this phasin to polyhydroxyalkanoic acid granules. | the function of the polyhydroxyalkanoic acid (pha) granule-associated ga14 protein of rhodococcus ruber was investigated in escherichia coli xl1-blue, which coexpressed this protein with the polyhydroxybutyric acid (phb) biosynthesis operon of alcaligenes eutrophus. the ga14 protein had no influence on the biosynthesis rate of phb in e. coli xl1-blue(pskco7), but this recombinant e. coli strain formed smaller phb granules than were formed by an e. coli strain that expressed only the phb operon. ... | 1995 | 7730285 |
| the evolutionary relationship of biphenyl dioxygenase from gram-positive rhodococcus globerulus p6 to multicomponent dioxygenases from gram-negative bacteria. | the gram+ bacterium rhodococcus globerulus p6 (rgp6) catabolizes a range of polychlorinated biphenyl (pcb) congeners, thus being of interest in bioelimination processes for pcb. the first step in the pathway, a dioxygenase attack of one of the biphenyl (bp) rings, is catalyzed by biphenyl dioxygenase (bdo). in this study, the nucleotide (nt) sequences of the four clustered cistrons, bpha1a2a3a4, encoding the subunits of bdo and forming part of the bph operon of rgp6 for bp degradation, were dete ... | 1995 | 7737502 |
| rhodococcus equi--an increasingly recognized opportunistic pathogen. report of 12 cases and review of 65 cases in the literature. | rhodococcus equi, a gram-positive, weakly acid-fast coccobacillus, initially isolated from horses, is becoming increasingly recognized as an important pathogen for immunosuppressed human hosts since the first human case was reported in 1967. a review of the english medical literature yielded 53 cases. during the last 11 years, the microbiology laboratories of the authors isolated the organism from 12 patients. of the total 65 cases, 60 occurred in immunosuppressed patients with hiv infection, ma ... | 1995 | 7741114 |
| disseminated abscesses caused by rhodococcus equi in a patient with chronic lymphocytic leukemia. | | 1995 | 7742468 |
| characterization of the bacillus stearothermophilus br219 phenol hydroxylase gene. | the catabolic genes phea and pheb, coding for the conversion of phenol to catechol and catechol to 2-hydroxymuconic semialdehyde, respectively, have been cloned from bacillus stearothermophilus br219 into escherichia coli. following its localization on the 11-kb b. stearothermophilus dna insert by deletion and expression analysis, the phenol hydroxylase gene phea was subcloned as a 2-kb hindiii fragment, whose transformant expressed the enzyme after phenol induction and even more strongly after ... | 1995 | 7747948 |
| cauda equina syndrome, diskospondylitis, and a paravertebral abscess caused by rhodococcus equi in a foal. | a 4-month-old male quarter horse was referred for evaluation of urinary incontinence. physical examination revealed clinical signs consistent with cauda equina syndrome. radiography revealed diskospondylitis of s2 through s4. infected bone was surgically curretted, and drainage was established for an associated paravertebral abscess. rhodococcus equi was isolated from specimens of bone and from fluid samples obtained from the paravertebral abscess. bethanechol was administered to stimulate urina ... | 1995 | 7751225 |
| characterization of muconate and chloromuconate cycloisomerase from rhodococcus erythropolis 1cp: indications for functionally convergent evolution among bacterial cycloisomerases. | muconate cycloisomerase (ec 5.5.1.1) and chloromuconate cycloisomerase (ec 5.5.1.7) were purified from extracts of rhodococcus erythropolis 1cp cells grown with benzoate or 4-chlorophenol, respectively. both enzymes discriminated between the two possible directions of 2-chloro-cis, cis-muconate cycloisomerization and converted this substrate to 5-chloromuconolactone as the only product. in contrast to chloromuconate cycloisomerases of gram-negative bacteria, the corresponding r. erythropolis enz ... | 1995 | 7751292 |
| chronic osteitis due to rhodococcus equi in an immunocompetent patient. | | 1995 | 7756506 |
| [the fist detection of giardia spp. in horses in the czech republic]. | the first occurrence of giardia spp. in horses in the czech republic is reported. during preventive examination of 360 five-month up to 14-year horses from various parts of the region of central bohemia carried out from january 1993 to june 1994 in the parasitological laboratory of the state veterinary institute in prague, the giardia cysts were detected in the excrements of 18 (5%) horses, mostly 2-4 years of age, and in two foals 3 and 6 weeks old. during the period between march 1993 and june ... | 1995 | 7762123 |
| development of an enzymatic process for manufacturing acrylamide and recent progress. | | 1993 | 7763351 |
| characterization of nitrile hydratase genes cloned by dna screening from rhodococcus erythropolis. | southern hybridization analysis using the genes encoding the alpha- and beta-subunits of nitrile hydratase (nhase) from rhodococcus sp. n-774 as probe suggested that two r. erythropolis strains, jcm6823 and jcm2892, among 31 strains mainly from japan culture of microorganisms (jcm) have nhase genes. restriction analysis of dna fragments showing positive hybridization showed that each fragment carried a nucleotide sequence very similar to that of the nhase genes from rhodococcus sp. n-774. nucleo ... | 1993 | 7764017 |
| production of s-(+)-2-phenylpropionic acid from (r,s)-2-phenylpropionitrile by the combination of nitrile hydratase and stereoselective amidase in rhodococcus equi tg328. | a new soil isolate, tentatively identified as rhodococcus equi tg328, was found to be effective in the production of s-(+)-2-phenylpropionic acid from (r,s)-2-phenylpropionitrile. the conversion is catalysed by two enzymes. first, a nitrile hydratase converts the (r,s)-nitrile to (r,s)-2-phenylpropionamide. second, a stereoselective amidase converts the s-(+)-amide to s-(+)-2-phenylpropionic acid. conditions for optimal enzyme production and accumulation of s-(+)-2-phenylpropionic acid by restin ... | 1993 | 7764117 |
| enantioselective hydrolysis of racemic naproxen nitrile and naproxen amide to s-naproxen by new bacterial isolates. | bacteria were enriched from soil samples with succinate as a carbon source and racemic naproxen nitrile [2-(6-methoxy-2-naphthyl)propionitrile] as sole source of nitrogen. since naproxen nitrile was only poorly soluble in water media amended with different water-immiscible organic phases were used for the enrichments. with pristane (2,6,10,14-tetramethylpentadecane) as the organic phase two bacterial strains were isolated (strain c3ii and strain mp50) which were identified as rhodococci. cells o ... | 1994 | 7764731 |
| purification and characterization of a novel carbonyl reductase isolated from rhodococcus erythropolis. | during growth on n-tetradecane a novel nadh-dependent carbonyl reductase is induced in the gram-positive bacterium rhodococcus erythropolis (peters, p., zelinski, t. and kula, m.r. (1992) appl. microbiol. biotechnol. 38, 334-340). the enzyme has been purified to homogeneity using fractional ph precipitation, anion exchange chromatography and affinity chromatography. the isoelectric point of the oxidoreductase is 4.4. the apparent molecular mass of the native enzyme is 161 kda, that of the subuni ... | 1994 | 7764739 |
| production of a bioflocculant by mixed culture. | colony groups that form large amounts of slime externally were obtained from activated sludge among phthalate-assimilating microbes. that slime, which r-3 mixed microbes produced externally, had a high level of flocculation activity. r-3 mixed strains, one of such colony groups, efficiently produced a bioflocculant (apr-3) in liquid cultures of production medium, especially that containing starch and glucose (1:1) as carbon sources. identification of this group of bioflocculant-producing microbe ... | 1994 | 7765478 |