| localization of viable bacteria and bacterial antigens in arthritic joints of erysipelothrix rhusiopathiae-infected pigs. | chronic polyarthritis was induced in pigs by infection with erysipelothrix rhusiopathiae (serovar 2, strain t28). viable bacteria could be reisolated as long as 5 months post-infection from synovial fluid, synovial tissue and from isolated chondrocytes. the number of viable bacteria could be increased by hypotonic shock of the chondrocytes indicating a substantial intracellular amount of bacteria. bacterial antigens were shown by immunohistochemistry to be present on the surface of both chondroc ... | 1995 | 8589663 |
| intracellular survival and replication of erysipelothrix rhusiopathiae within murine macrophages: failure of induction of the oxidative burst of macrophages. | we investigated the ability of a virulent wild-type parent strain and acapsular avirulent transposon mutants to enter and survive intracellularly within murine peritoneal macrophages. in the presence of normal or immune serum, the parent and mutant strains were both ingested; however, the number of ingested bacteria was three- to fourfold greater in the case of mutant strains than in the case of the parent strain. the parent strain, but not the mutant strains, survived and replicated intracellul ... | 1996 | 8613392 |
| growth ability and immunological properties of erysipelothrix rhusiopathiae serotype 2. | five field strains of erysipelothrix rhusiopathiae belonging to serotype 2 were compared for their growth ability, immunogenicity in mice, sds-page profile of cell surface proteins and their immunoblotting patterns. strain tama-96 showed the most stable growth in feist medium and tryptose phosphate broth with tween 80 (tpb), and its immunogenicity was highest in a mouse protection test using the inactivated vaccines prepared from 20-h tpb culture. the 50% mouse protective dose of the vaccine was ... | 1996 | 8645767 |
| [bacteremia caused by erysipelothrix rhusiopathiae in an hiv-positive patient]. | | 1996 | 8756229 |
| endocarditis and acute renal failure due to erysipelothrix rhusiopathiae. | | 1996 | 8781890 |
| serovars of erysipelothrix strains isolated from pigs affected with erysipelas in japan. | serovars of 1,046 erysipelothrix strains isolated from diseased pigs during a period from 1983 to 1993 in japan were determined by an agar gel double-diffusion precipitation system using typing sera representing all the known serovars, 1 through 23 and type n, of 2 species of erysipelothrix. a total of 943 strains could be serotyped within the serovars. the remaining 103 strains could not be determined for their serovars and were classified as untypable. of the 938 (99.5%) strains serologically ... | 1996 | 8811634 |
| non-purulent arthritis in danish slaughter pigs. i. a study of field cases. | the pathology and microbiology of non-purulent arthritis in danish slaughter pigs were studied in order to draw up an updated picture of the disease. samples of one or more joints, their regional lymph nodes and the heart were collected from 101 arthritic pigs, totalling 137 joints, 86 lymph nodes and 96 hearts (group i). for another selected group including 25 pigs (group ii), the preliminary diagnosis of mycoplasmic arthritis, based on the appearance of the corresponding regional lymph node, w ... | 1995 | 8822187 |
| acute oliguric renal failure in a patient with an erysipelothrix rhusiopathiae bacteremia and endocarditis. | | 1996 | 8883054 |
| erysipelothrix rhusiopathiae septicemia in systemic lupus erythematosus. | | 1996 | 8915741 |
| characterization of spaa, a streptomyces coelicolor gene homologous to a gene involved in sensing starvation in escherichia coli. | a streptomyces coelicolor gene, called spaa, homologous to the stationary phase regulatory gene rspa of escherichia coli [huisman and kolter (1994) science 265, 537-539], was cloned using the streptomyces ambofaciens rspa homologue spa2 [schneider et al. (1993) j. gen. microbiol. 139, 2559-2567] as a probe. considerable differences in sequence and in genetic context were detected between spa2 of s. ambofaciens and spaa of s. coelicolor. a cloned internal fragment of spaa was used to direct integ ... | 1996 | 8921874 |
| [aortic endocarditis following erysipelothrix rhusiopathiae septicemia]. | | 1996 | 8949797 |
| [erysipelothrix rhusiopathiae: plasmids, resistance to antibacterial drugs]. | the plasmid profile, virulence and antibacterial drug susceptibility of various strains of e. rhusiopathiae were determined. no correlations between the virulence of the strains, their antibiotic resistance and the plasmid content were detected. structural and functional analysis of one of the isolated plasmids was carried out to use the plasmid as a vector in the genetic study of e. rhusiopathiae. | 1996 | 8967808 |
| [septic arthritis caused by erysipelothrix rhusiopathiae]. | | 1996 | 8991446 |
| septicaemic erysipelothrix rhusiopathiae infection in the little swift (apus affinis). | erysipelothrix rhusiopathiae was found to be the causal organism of high mortality in a colony of little swifts (apus affinis (gray), occupying the vertical walls of high-rise buildings. the mortality continued for a period of about 4 weeks. negative post-mortem findings necessitated a diagnosis based on bacterial examination during which the causal organism was isolated in pure culture from the liver, spleen and heart blood of affected birds. | 1990 | 9022847 |
| bacterial isolation from slaughtered pigs associated with endocarditis, especially the isolation of streptococcus suis. | bacterial isolation from slaughtered pigs with endocarditis was carried out from 1985 to 1994. a total of 495 (0.025%) out of 2,006,127 pigs were diagnosed as having endocarditis. though bacteria were significantly isolated from 399 of the 495 pigs, bacteria could not be isolated in 96 pigs (19.4%). in 11 pigs, 2 bacterial species were isolated from heart lesion. streptococcus suis was isolated from 127 cases (25.7%), streptococcus dysgalactiae from 75 (15.2%), erysipelothrix rhusiopathiae from ... | 1997 | 9035085 |
| molecular cloning and analysis of a hsp (heat shock protein)-like 42 kda antigen gene of mycoplasma hyopneumoniae. | the recombinant clone expressing the 42 kda protein (p42) of mycoplasma hyopneumoniae in escherichia coli was analyzed. the 4.4 kb hindiii-xmal dna fragment expressing the p42 gene product encodes three orfs: p42 and p16 in the forwarding strand, p24 in the reverse strand. sequence comparisons revealed that p42 could be part of a p65 gene, and has 62% identities with mycoplasma genitalium hsp70 gene and 56% identities with bacillus subtilis dnak gene; p16 and p24 genes share 73% and 47% identiti ... | 1997 | 9111943 |
| erysipelothrix rhusiopathiae septicemia in a neonate. | | 1997 | 9114208 |
| meat safety consequences of implementing visual postmortem meat inspection procedures in danish slaughter pigs. | the consequences of a change from a traditional meat inspection procedure, including manual handling, palpation and incision, to an entirely visual postmortem meat inspection procedure in danish slaughter pigs were assessed by a comparative study of the two methods in 183,383 slaughter pigs. out of 58 lesion codes (selected with a prevalence > or = 5.5 x 10(-5)), 26 (45 per cent) were assessed either as merely aesthetic or as the healed stage of an earlier lesion and nine (15 per cent) as active ... | 1997 | 9160530 |
| [erysipelothrix rhusiopathiae bacteremia after dog bite]. | a case of erysipeloid with bacteraemia caused by erysipelothrix rhusiopathiae (er) in a previously healthy 41-year old man is presented. the bacterium was probably introduced by the bite of a dog. he was treated successfully with penicillin v. the er bacteraemia occurred without complications of endocarditis. | 1997 | 9235737 |
| infective endocarditis caused by erysipelothrix rhusiopathiae in a patient with systemic lupus erythematosus. | | 1996 | 9251330 |
| phenotypic and phylogenetic characterization of some eubacterium-like isolates containing a novel type b wall murein from human feces: description of holdemania filiformis gen. nov., sp. nov. | a group of eubacterium-like strains (designated group s14), isolated from the feces of healthy people, was characterized by biochemical tests, fatty acid analysis, cell wall murein analysis, and 16s rdna analysis. our results indicate that group s14 is phylogenetically a member of the clostridium subphylum of the gram-positive bacteria. despite a phenotypic resemblance to the genus eubacterium, group s14 was shown to be phylogenetically distantly related to the type species of the genus, eubacte ... | 1997 | 9336928 |
| concurrent infection with enteric protozoa and erysipelothrix rhusiopathiae in chicken and pheasant flocks. | | 1997 | 9347625 |
| disseminated intravascular coagulation in chickens inoculated with erysipelothrix rhusiopathiae. | in a first experiment, 28 specific pathogen-free chickens aged 3 weeks showed clinical signs 1 to 5 days after intramuscular inoculation with erysipelothrix rhusiopathiae. twelve of 28 birds died 2 to 4 days after inoculation. macroscopically, the liver, spleen and kidneys were seen to be enlarged and congested. histologically, fibrinous thrombus formation, seen in the hepatic sinusoids, renal glomerular capillaries and small pulmonary blood vessels, was a characteristic feature. in addition, th ... | 1997 | 9352439 |
| erysipelothrix rhusiopathiae endocarditis: clinical features of an occupational disease. | erysipelothrix rhusiopathiae is becoming more commonly recognized in humans and has the potential for significant morbidity and mortality. in this article, we describe one patient's clinical symptoms after occupational exposure to e rhusiopathiae and its sequela. we discuss the natural history of the organism, three major categories of human disease, and treatment options. | 1997 | 9386061 |
| effect of attenuated erysipelothrix rhusiopathiae vaccine in pigs infected with porcine reproductive respiratory syndrome virus. | twenty 2nd specific pathogen-free pigs were divided into 4 groups: group a were infected with porcine reproductive and respiratory syndrome (prrs) virus at 6 weeks of age and treated with available swine erysipelas and swine fever combined vaccine (vaccinated) at 7 weeks of age; group b were vaccinated at 7 weeks of age and infected with prrs virus at 8 weeks of age; group c were vaccinated at 7 weeks of age: group d were neither vaccinated nor infected with prrs virus. all pigs were challenged ... | 1997 | 9409511 |
| use of an enrichment broth cultivation-pcr combination assay for rapid diagnosis of swine erysipelas. | we have previously described the creation by tn916 mutagenesis of avirulent transposition mutants from a highly virulent strain of erysipelothrix rhusiopathiae, the causative agent of swine erysipelas. in this study, we cloned a 2.2-kb dna fragment which flanked the tn916 insertion in an avirulent mutant (strain 33h6) and evaluated the possibility that this region could be used for the specific detection of e. rhusiopathiae. according to the sequences of this region, oligonucleotide primers were ... | 1998 | 9431926 |
| typing of staphylococcus aureus isolated from nasal carriers. | eighteen staphylococcus aureus strains (9 pairs) were isolated from 9 apparently healthy persons (including 5 persons from medical staff) regarded as persistent nasal carriers in a 5-7-day or 3-5-month period, respectively. the 18 s. aureus strains were characterized phenotypically and by genotypic methods. biochemical properties determined with a commercial identification system, production of haemolysins as well as most of the antibiotic resistance date revealed an identity between both strain ... | 1997 | 9440205 |
| protective effect of naoh-extracted erysipelothrix rhusiopathiae vaccine in pigs. | a vaccine was prepared from a naoh-extracted antigen of the kyoto strain (serovar 2) of erysipelothrix rhusiopathiae (e. rhusiopathiae) with an oil adjuvant, and was injected twice at 3-week intervals into spf pigs and conventional pigs with maternal antibodies. after the second vaccination, igg-ga titers of immunized spf pigs were more than 256-fold at 3 weeks, and immunized pigs with maternal antibodies were 64-fold at 7 weeks. the pig with maternal antibodies vaccinated once with live vaccine ... | 1998 | 9492354 |
| fatal erysipelothrix rhusiopathiae septicemia in a captive pacific white-sided dolphin (lagenorhyncus obliquidens). | one male of a group of seven pacific white-sided dolphins (lagenorhynchus obliquidens) died after a brief period of nonspecific clinical signs. four beluga whales (delphinapterus leucas) and four harbor seals (phoca vitulina) were managed in the same water system. gross examination of the dolphin revealed only moderately enlarged mesenteric lymph nodes. histopathology revealed small to massive numbers of gram-positive bacilli, usually intravascular, in all tissues. bacteria were both extracellul ... | 1997 | 9523647 |
| acute nonfatal erysipelas in sows in a commercial farrow-to-finish operation. | acute nonfatal erysipelas was diagnosed in 4 sows in a 1,000-sow commercial farrow-to-finish operation in indiana. sows were pyrexic, lethargic, lame, and had multiple, 1.3- to 7.6-cm, erythematous rhomboid skin lesions. outbreak was attributed to failure to properly vaccinate pigs. further morbidity and mortality were prevented by treatment of clinically affected sows and all pigs in close proximity with procaine penicillin g daily for 3 consecutive days and vaccination of all pigs with questio ... | 1998 | 9524646 |
| comparison of the protein patterns of erysipelothrix rhusiopathiae strains by sds-page and autoradiography. | the proteins of 12 erysipelothrix rhusiopathiae strains were radiolabelled with l-[35s] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and autoradiography. the strains were found to differ from each other in their protein patterns; thus, it was concluded that the method applied could provide useful data for the identification of strains. the described procedure was used for determining the percentage contents of protein ... | 1997 | 9557319 |
| in vitro susceptibilities of aerobic and facultative non-spore-forming gram-positive bacilli to hmr 3647 (ru 66647) and 14 other antimicrobials. | the comparative in vitro activity of the ketolide hmr 3647 (ru 66647) and those of structurally related macrolide-lincosamide-streptogramin compounds (erythromycin, roxithromycin, azithromycin, clarithromycin, josamycin, lincomycin, pristinamycin, and quinupristin-dalfopristin) as well as those of benzylpenicillin, doxycycline, vancomycin, teicoplanin, levofloxacin, and rifapentine against 247 aerobic and facultative non-spore-forming gram-positive bacilli were determined by an agar dilution met ... | 1998 | 9593121 |
| superoxide release in human fibroblasts upon treatment with culture supernatants of the arthritogenic bacteria erysipelothrix rhusiopathiae and mycoplasma arthritidis. | culture supernatants of the arthritogenic bacteria mycoplasma pneumonia, mycoplasma arthritidis, borrelia burgdorferi and erysipelothrix rhusiopathiae stimulated primary cultures of human fibroblasts to release reactive oxygen species into the environment, whereas cell walls and membranes of these bacteria had no effects. lipopolysaccharides of various gram-negative bacteria and lipid a, the lipid moiety of endotoxines, also failed to stimulate the release of reactive oxygen species by fibroblas ... | 1998 | 9618939 |
| construction and vaccine potential of acapsular mutants of erysipelothrix rhusiopathiae: use of excision of tn916 to inactivate a target gene. | we previously showed that acapsular transposon tn916 mutants of erysipelothrix rhusiopathiae are avirulent for mice. in this study, we constructed nonreverting acapsular mutants and examined the vaccine potential of the mutants in mice. a representative acapsular transposon mutant, 33h6, was plated on selective agar containing autoclaved chlortetracycline and quinaldic acid, and two tetracycline-sensitive mutants were obtained. sequence analysis of chromosomal regions of the mutants in which tn9 ... | 1998 | 9632592 |
| occurrence of erysipelothrix spp. in broiler chickens at an abattoir. | from september 1995 to august 1996, 750 chickens from 66 farms sent to an abattoir in nagano prefecture, japan, were examined for the presence of erysipelothrix spp. erysipelothrix spp. were isolated from 118 (15.7%) of 750 skin samples, 27 (7.3%) of 372 hypoderm samples, 12 (1.9%) of 630 throat samples, 106 (59.2%) of 179 feather samples, and none of 257 spleen samples. of 66 farms, 55 farms (83.3%) sent erysipelothrix-positive chickens and 11 farms (16.7%) only negative ones. of 297 erysipelot ... | 1998 | 9678179 |
| cross protection against erysipelothrix rhusiopathiae serotype 10 induced by a serotype 1 and 2 vaccine. | | 1998 | 9682422 |
| comparison of the protein composition of erysipelothrix rhusiopathiae strains of subtype 1a isolated from ducks and pigs. | erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate--polyacrylamide gel electrophoresis (sds-page) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular mass. to characterise the similarity of the strains, pearson's correlation coefficient was calculated. the degree of similarity between duck strains originating from the same flock was 980-991, while th ... | 1998 | 9704524 |
| properties of repeat domain found in a novel protective antigen, spaa, of erysipelothrix rhusiopathiae. | erysipelothrix rhusiopathiae is a small gram-positive rod bacterium that causes erysipelas in swine and a variety of diseases in other animals and humans. although live-attenuated or bacterin vaccines are effective in protecting against erysipelas, the genetic construction of their active antigen has not been identified. to clarify the surface antigen(s) involved in protective and arthritic response, using monoclonal antibody i2a against the surface proteins of e. rhusiopathiae, we identified a ... | 1998 | 9712689 |
| an outbreak of urticarial form of swine erysipelas in a medium-scale piggery in kiambu district, kenya. | this report concerns an outbreak that occurred during july/august 1997. ten pigs from a herd of 181 pigs in a medium-scale, semi-closed piggery in kiambu district, kenya, contracted the clinical disease. the main clinical findings in affected pigs included: fever (40.5-41.8 degrees c), prostration, inappetence, dog-sitting posture, abortion, erythema and raised, firm to the touch and easily palpated light pink to dark purple diamond-shaped to square/rectangular spots on the skin around the belly ... | 1998 | 9760400 |
| effects of tamoxifen, melatonin, coenzyme q10, and l-carnitine supplementation on bacterial growth in the presence of mycotoxins. | the involvement of toxic oxygen intermediates in the bacteriostatic effects of mycotoxins (t-2 toxin, deoxynivalenol, ochratoxin a, aflatoxin b1, and fumonisin b1) was investigated by producing bacterial growth curves using turbidimetry assays in the presence and absence of oxygen radical-scavenging substances. the strains used in this study included escherichia coli (ft 101), streptococcus agalactiae (ft 311, ft 313, ft 315), staphylococcus aureus (ft 192), yersinia enterocolitica (ft 430), sal ... | 1998 | 9774492 |
| use of the protective antigen of erysipelothrix rhusiopathiae in the enzyme-linked immunosorbent assay and latex agglutination. | to establish a safe and convenient serodiagnostic method for swine erysipelas, a purified protective protein antigen of erysipelothrix rhusiopathiae, which included a large amount of protective protein (64 kda protein), was used for enzyme-linked immunosorbent assay (elisa) and the latex agglutination (la) test. in the elisa, the antisera to four different serovars (1a, 2, 5 and 20) of e. rhusiopathiae exhibit a positive reaction, while antisera to other species of bacteria (listeria monocytogen ... | 1998 | 9780828 |
| isolation of acriflavine resistant erysipelothrix rhusiopathiae from slaughter pigs in japan. | erysipelothrix rhusiopathiae is the causative agent of swine erysipelas. although an attenuated vaccine is used in japan, recent increases in disease occurrence have cast doubts on its efficacy. we investigated the similarity between the vaccine strain and e. rhusiopathiae field isolates by the analysis of acriflavine resistance (the vaccine strain marker), serotype, dna fingerprinting and pathogenicity to mice. although 7 acriflavine resistant e. rhusiopathiae isolates were separated from arthr ... | 1998 | 9795903 |
| measurement of antibacterial activities of t-2 toxin, deoxynivalenol, ochratoxin a, aflatoxin b1 and fumonisin b1 using microtitration tray-based turbidimetric techniques. | various mycotoxins were tested for their antibacterial activity by evaluating growth delays using a fully automated microturbidmetric method. ten different strains of the genera escherichia, streptococcus, staphylococcus, yersinia, salmonella, erysipelothrix and lactobacillus were used as test micro-organisms. t-2 toxin, deoxynivalenol (don), ochratoxin a (ota), aflatoxin b1 (afb1) and fumonisin b1 (fb1) were used as representative mycotoxins. the inhibitory effect in vitro was defined as the di ... | 1998 | 9838857 |
| erysipelothrix rhusiopathiae: an uncommon but ever present zoonosis. | erysipelothrix rhusiopathiae is an important animal pathogen with a worldwide distribution, yet this zoonotic infection is rarely reported in humans. three cases of e. rhusiopathiae infection, which illustrate the varied clinical presentations of this pathogen in humans, are presented together with the pathological findings and treatment regimens. | 1998 | 9839315 |
| computerized evaluation procedure for comparing the electrophoretic protein patterns of bacterial strains. | the advantages and drawbacks of different methods of evaluating electrophoretic (sds-page) protein patterns were studied by comparing erysipelothrix rhusiopathiae strains. the computerized evaluation procedure recommended by the authors renders it possible to determine objectively the degree of similarity of the protein composition of the different strains according to molecular mass. the degree of similarity obtained in four parallel analyses of a given strain was 988 +/- 0.55 on a scale on whi ... | 1998 | 9867344 |
| bacteriologic analysis of infected dog and cat bites. emergency medicine animal bite infection study group. | to define better the bacteria responsible for infections of dog and cat bites, we conducted a prospective study at 18 emergency departments. to be eligible for enrollment, patients had to meet one of three major criteria for infection of a bite wound (fever, abscess, and lymphangitis) or four of five minor criteria (wound-associated erythema, tenderness at the wound site, swelling at the site, purulent drainage, and leukocytosis). wound specimens were cultured for aerobic and anaerobic bacteria ... | 1999 | 9887159 |
| immunological characterization of a protective antigen of erysipelothrix rhusiopathiae: identification of the region responsible for protective immunity. | the gene encoding a protective protein antigen of the gram-positive bacterium erysipelothrix rhusiopathiae, an important veterinary pathogen responsible for erysipelas in swine and a variety of diseases in animals, was cloned and sequenced. the gene encodes a polypeptide of 597 amino acids plus a putative signal sequence of 29 amino acids, resulting in a mature protein with a molecular mass of 69,017 da. sequence analysis of the gene product revealed a c-terminal region composed of nine tandem r ... | 1999 | 10084998 |
| protective activity of the purified protein antigen of erysipelothrix rhusiopathiae in pigs. | we purified the protein antigen (p64), which contains 66 and 64 kda proteins, from the alkaline extract (ae) of whole cells of erysipelothrix rhusiopathiae strain agata (serovar 5) to determine the protective activity of the antigen against e. rhusiopathiae infection in pigs. the serum titre of antibody against p64 rapidly increased in pigs immunized with 500 and 100 micrograms of p64 and reached maximum values at 3 weeks after the first immunization (1 week after the second immunization). howev ... | 1999 | 10085773 |
| isolation and purification of a protective protein antigen of erysipelothrix rhusiopathiae. | the rapid growth and high survival rate of erysipelothrix rhusiopathiae was determined using a culture of the bacterium in tryptic soy broth supplemented with 0.3% tris-hydroxymethyl aminomethane and 0.1% tween 80 (tt-ts broth). high concentrations of 64, 66 and 43 kda proteins, which are associated with protection against e. rhusiopathiae infection in mice, were obtained by alkaline treatment of whole cells using 0.05-1 n naoh. the supernatant of alkaline treated cells (alkaline extract; ae) wa ... | 1999 | 10216450 |
| preparation and partial characterization of monoclonal antibodies against the protective protein antigen of erysipelothrix rhusiopathiae. | thirteen mouse monoclonal antibodies (mabs) against the protective protein antigen (p64) of erysipelothrix rhusiopathiae were prepared and partially characterized. the titres of the mabs varied from 200 to 1,638,400 as determined by enzyme-linked immunosorbent assay (elisa). of the 13 mabs 10, two and one belonged to the igg2a, igg1 and igm subclasses, respectively. all mabs reacted strongly with the 64 kda protein and weakly with the 43 kda protein upon western blotting of the alkaline extract ... | 1999 | 10216451 |
| [comparative investigations of a combined vaccine against parvovirus and erysipelas and corresponding monovaccines in different vaccination schedules. 1: field trial]. | in a field trial, the development of antibodies of a combined vaccine against the porcine parvovirus (ppv) as well as against swine erysipelas was compared with corresponding mono vaccines. furthermore, these vaccines were used in different vaccination schedules. the tests were carried out on 109 gilts in three closed farms. in all gilts, a basic immunization repeated twice was carried out at the age of six months and at intervals of three weeks. the revaccination was carried out four months aft ... | 1999 | 10384706 |
| erysipelas in a free-ranging hawaiian crow (corvus hawaiiensis). | we describe a case of erysipelas in a free-ranging endangered hawaiian crow. the partially scavenged carcass exhibited gross emaciation and petechial hemorrhages in both lungs. microscopy revealed multiple necrotic foci associated with gram-positive rods in the liver and adrenal, diffuse acute proximal tubular necrosis of kidney, diffuse necrosis and inflammation of proventricular mucosa associated with gram-positive rods, and multiple intravascular aggregates of gram-positive rods associated wi ... | 1999 | 10396650 |
| a novel protein of erysipelothrix rhusiopathiae that confers haemolytic activity on escherichia coli. | erysipelothrix rhusiopathiae, the cause of swine erysipelas and human erysipeloid, produces a haemolysin. a recombinant plasmid, phly, conferring haemolytic activity on escherichia coli was isolated from a genomic library of ery. rhusiopathiae strain tama-96. this plasmid was stable in reca- e. coli, but unstable in a reca+ strain. a spontaneous deletion plasmid, pmini-hly, also conferring haemolytic activity was derived from phly. two orfs were detected in phly. analysis of pmini-hly and other ... | 1999 | 10411263 |
| home intravenous antibiotic therapy for patients with infective endocarditis. | although home intravenous antibiotic therapy (hiat) is increasingly being used for various infectious diseases, outpatient treatment of infective endocarditis (ie) is still uncommon. recently, the american heart association recommended outpatient treatment of endocarditis only for infections with streptococci that are highly susceptible to penicillin. herein, the experience with hiat in patients with ie due to a diversity of pathogens is presented. during a 3-year period, 37 patients with ie who ... | 1999 | 10421039 |
| truncated surface protective antigen (spaa) of erysipelothrix rhusiopathiae serotype 1a elicits protection against challenge with serotypes 1a and 2b in pigs. | erysipelothrix rhusiopathiae is a causal agent of swine erysipelas, which is of economic importance in the swine industry by virtue of causing acute septicemia, chronic arthritis, and endocarditis. however, little is known about the genetic properties of its protective antigens. recently, a surface protective antigen (spaa) gene was identified from serotype 2 in a mouse model. we cloned spaa from virulent strain fujisawa (serotype 1a) and determined that the n-terminal 342 amino acids without c- ... | 1999 | 10456877 |
| erysipelothrix rhusiopathiae: bacteriology, epidemiology and clinical manifestations of an occupational pathogen. | erysipelothrix rhusiopathiae has been recognised as a cause of infection in animals and man since the late 1880s. it is the aetiological agent of swine erysipelas, and also causes economically important diseases in turkeys, chickens, ducks and emus, and other farmed animals such as sheep. the organism has the ability to persist for long periods in the environment and survive in marine locations. infection in man is occupationally related, occurring principally as a result of contact with animals ... | 1999 | 10482289 |
| valvular and myocardial abscesses due to erysipelothrix rhusiopathiae. | | 1999 | 10525004 |
| serotyping and pathogenicity of erysipelothrix strains isolated from tonsils of slaughter pigs in thailand. | erysipelothrix strains were isolated from the tonsils of 46 (15.0%) of 307 apparently healthy slaughter pigs in thailand during the period of august to september, 1997. a total of 27 of the 46 erysipelothrix isolates could be classified into 5 serovars but the remaining 19 were untypable in this study. of the 25 isolates serologically identified as erysipelothrix rhusiopathiae, 20, 4, and 1 isolates belonged to serovars 2, 12, and 17, respectively. only 2 isolates from the tonsils belonged to er ... | 1999 | 10535518 |
| direct and rapid detection by pcr of erysipelothrix sp. dnas prepared from bacterial strains and animal tissues. | a pcr method for rapid screening of erysipelothrix spp. in the slaughterhouse was carried out by using four species-specific sets of oligonucleotide primers after initial amplification with the primer set mo101-mo102, which amplifies the 16s rrna sequences of all four erysipelothrix species. the dna sequences coding for the rrna gene cluster, including 16s rrna, 23s rrna, and the noncoding region downstream of 5s rrna, were determined in order to design primers for the species-specific pcr detec ... | 1999 | 10565937 |
| [erysipelothrix rhusiopathiae]. | | 1996 | 10605243 |
| serovar, pathogenicity and antimicrobial susceptibility of erysipelothrix rhusiopathiae isolates from farmed wild boars (sus scrofa) affected with septicemic erysipelas in japan. | six strains of erysipelothrix rhusiopathiae were isolated from farmed wild boars with acute septicemic erysipelas during the period from 1983 to 1998 in japan. all isolates belonged to serovar 1a or 2 (predominant serovars in swine). the 50 per cent lethal dose values of those isolates ranged from 10(1.3)to 10(6.2)colony forming units in mice. in swine, all isolates were virulent, capable of inducing localized or generalized urticarial lesions after intradermal inoculation. all of the isolates w ... | 1999 | 10607512 |
| [erysipelas of the skin in fattening swine: assessment from the view of meat hygiene]. | nine pigs with acute erysipelas in skin manifestation have been subjected in 1998 to a bacteriological examination. eight animals proved to have erysipelothrix rhusiopathiae in muscles and/or organs. in the part of the well-defined rhomboid, bluish-red skin lesions, histology showed a disseminated acute vasculitis and a perivascular suppurating dermatitis and panniculitis. the actually valid swiss meat inspection ordinance which in case of erysipelas in skin manifestation requires only the elimi ... | 1999 | 10624152 |
| potential errors in recognition of erysipelothrix rhusiopathiae. | here we describe four isolations of erysipelothrix rhusiopathiae associated with polyarthralgia and renal failure, septic arthritis, classic erysipeloid, and peritonitis. although the biochemical identification was straightforward in each case, recognition presented a challenge to the clinical microbiologist, since in three cases e. rhusiopathiae was not initially considered due to unusual clinical presentations, in two cases the significance might not have been appreciated because growth was in ... | 2000 | 10699048 |
| enterococcal-type glycopeptide resistance genes in non-enterococcal organisms. | although the emergence of vancomycin-resistant enterococci can be attributed, in part, to the increasing use of vancomycin in clinical practice, and glycopeptide use in animal husbandry, the origins of the enterococcal vancomycin resistance genes are not clear. the vancomycin resistance-associated genes in enterococcus gallinarum, enterococcus casseliflavus/flavescens, lactobacillus spp., leuconostoc spp., pediococcus spp., and erysipelothrix rhusiopathiae, are not the source of the high-level v ... | 2000 | 10731599 |
| [erysipelothrix rhusiopathiae bacteremia in a child with acute leukemia]. | the systemic infections caused by e. rhusiopathiae and reported in literature are very rare, majority evolving with endocarditis. the authors present a child with acute leukemia, to whom the blood culture during a high temperature episode allows to isolate this bacterium, without valvular lesions. the case is interesting because of the arisen bacteriological diagnosis problems and because of the pathogenic aspects of the infection. an animal source contact being absent, we took in account the in ... | 1997 | 10756759 |
| comparison of methods for detection of erysipelothrix spp. and their distribution in some australasian seafoods. | for many years, erysipelothrix rhusiopathiae has been known to be the causative agent of the occupationally related infection erysipeloid. a survey of the distribution of erysipelothrix spp. in 19 australasian seafoods was conducted, and methodologies for the detection of erysipelothrix spp. were evaluated. twenty-one erysipelothrix spp. were isolated from 52 seafood parts. primary isolation of erysipelothrix spp. was most efficiently achieved with brain heart infusion broth enrichment followed ... | 2000 | 10788383 |
| surface antigen, spaa, of erysipelothrix rhusiopathiae binds to gram-positive bacterial cell surfaces. | in a previous study, we isolated the spaa gene encoding the surface protective antigen a, spaa, of erysipelothrix rhusiopathiae, and found that the n-terminal region of spaa was responsible for protective immunity against erysipelas and that the c-terminal region contained eight repeat units consisting of 20 amino acids comprising the binding domain on the erysipelothrix cell surface. in this study, using recombinant spaa proteins, we showed that the repeat region bound to the cell surfaces of v ... | 2000 | 10802190 |
| phylogenic and phenotypic characterization of some eubacterium-like isolates from human feces: description of solobacterium moorei gen. nov., sp. nov. | three isolated strains from human feces were characterized by biochemical tests and 16s rdna analysis. phylogenetic analysis revealed that these isolated strains were members of the clostridium subphylum of gram-positive bacteria. the phenotypic characters resembled those of the genus eubacterium, but these strains were shown to be phylogenetically distant from the type species of the genus, eubacterium limosum. the strains showed a specific phylogenetic association with holdemania filiformis an ... | 2000 | 10832964 |
| bulleidia extructa gen. nov., sp. nov., isolated from the oral cavity. | five strains of anaerobic non-sporing gram-positive bacilli isolated from advanced periodontitis (four strains) and a dentoalveolar abscess (one strain) that did not correspond to existing species were subjected to phenotypic and genetic characterization. following 16s rdna sequence analysis, they were found to constitute a novel branch of the low g+c gram-positive division of the phylogenetic tree related to erysipelothrix rhusiopathiae and holdemania filiformis. a new genus bulleidia, and the ... | 2000 | 10843035 |
| taxonomic evidence that serovar 7 of erysipelothrix strains isolated from dogs with endocarditis are erysipelothrix tonsillarum. | the levels of relatedness among strains of erysipelothrix serovar 7 isolated from dogs with endocarditis were estimated by performing dna-dna hybridization experiments with the type strains of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum. all the canine strains exhibited more than 81% hybridization with the type strain of e. tonsillarum but less than 13% hybridization with the type strain of e. rhusiopathiae. based on dna-dna hybridization results we confirmed that serovar 7 of th ... | 2000 | 10861200 |
| fatal outcome of erysipelothrix rhusiopathiae bacteremia in a patient with oropharyngeal cancer. | bacteremia due to erysipelothrix rhusiopathiae is rare; the most common presentation reported in the literature is endocarditis. we report a 32-year-old man with oropharyngeal cancer who developed aspiration pneumonia and e. rhusiopathiae bacteremia, and presented with fever, chills, dyspnea, and productive cough with purulent sputum. despite treatment with amoxicillin/clavulanate and nutritional support for 9 days, he died of respiratory failure. he had no clinical evidence of endocarditis. he ... | 2000 | 10870336 |
| [endocarditis caused by erysipelothrix rhusiopathiae]. | | 2000 | 10893780 |
| pathogenicity of erysipelothrix rhusiopathiae: virulence factors and protective immunity. | erysipelothrix rhusiopathiae is the causative agent of erysipelas in animals and erysipeloid in humans. in the absence of specific antibodies, the organism evades phagocytosis by phagocytic cells, but even if phagocytized, it is able to replicate intracellulary in these cells. in this review, recent advances in our understanding of the pathogenicity of e. rhusiopathiae and its protective immunity are described. | 2000 | 10962280 |
| an outbreak of erysipelas in 2-day-old poults. | systemic erysipelas infection was seen in 2-to-4-day-old poults from three separate ranches owned by the same company. the affected poults were all from the same breeder source; poults from other breeder sources were seemingly unaffected. mortality increased on days 2 and 3, ranging from 2% to 8.5%. birds submitted were dehydrated and very weak, with one half of the poults submitted having died during transport to the lab. gross lesions included swollen, congested livers and spleens, as well as ... | 2000 | 11007027 |
| influence of antibiotics used as feed additives on the immune effect of erysipelas live vaccine in swine. | to investigate the influence of antibiotics used as feed additives on the immune response to erysipelas live vaccine, the pig inoculation test was applied. avilamycin, oxytetracycline quaternary salt, enramycin, virginiamycin and tylosin phosphate were selected as test antibiotics. five experimental feeds containing each antibiotic at the highest concentration permitted for feed additives in japan, and the basal diet lacking antibiotics were examined. twenty-nine pigs were divided into six group ... | 2000 | 11014067 |
| quantitative diversity of 67 kda protective antigen among serovar 2 strains of erysipelothrix rhusiopathiae and its implication in protective immune response. | mouse monoclonal antibodies (mabs), raised against the naoh-extracted antigen of erysipelothrix rhusiopathiae strain kyoto (serovar 2), recognized two different epitopes on a single protein of molecular weight 67 kda. the mabs were classified as protective or non-protective against strain fujisawa (serovar 1). in immunoblotting analysis using the mabs, fifteen wild strains were shown to contain different amounts of 67 kda protective antigen. each formalin-killed whole cell vaccine (bacterin) pre ... | 2000 | 11073078 |
| phylogenetic analysis of the erysipelothrix rhusiopathiae and erysipelothrix tonsillarum based upon 16s rrna. | the nucleotide sequences of 16s rrna genes of erysipelothrix rhusiopathine and erysipelothrix tonsillarum were determined. the sequences are almost similar (99.8%) with only three nucleotides mismatched. | 2000 | 11092736 |
| topically acquired bacterial zoonoses from fish: a review. | the main pathogens acquired topically from fish (through spine puncture or open wounds) are aeromonas hydrophila, edwardsiella tarda, erysipelothrix rhusiopathiae, mycobacterium marinum, streptococcus iniae, vibrio vulnificus and vibrio damsela. s. iniae has recently emerged as a public health hazard associated with aquaculture, and m. marinum often infects home aquarium hobbyists. with the expansion of aquaculture and popularity of recreational fishing in australia, medical practitioners can ex ... | 2000 | 11130351 |
| comparison of dna sequencing of the protein a gene polymorphic region with other molecular typing techniques for typing two epidemiologically diverse collections of methicillin-resistant staphylococcus aureus. | the aim of this study was to compare the recently developed typing approach for methicillin-resistant staphylococcus aureus (mrsa) based on the dna sequencing of the protein a gene polymorphic region (spaa typing) with a combination of three well-established molecular typing techniques: clai-meca vicinity polymorphisms, clai-tn554 insertion patterns, and smai pulsed-field gel electrophoresis (pfge) profiles. in order to evaluate the applicability of this typing technique in different types of st ... | 2001 | 11158109 |
| [newer knowledge concerning a protective antigen of erysipelothrix rhusiopathiae] | investigations on extracts from erysipelothrix (e.) rhusiopathiae carried out within the last few years yielded the identification of a species-specific proteinaceous antigen of 66-64 kda. the protective properties of this protein presented in particular in crude naoh and naoh-edta but not in acid and heat extracts could be demonstrated in mice and pigs vaccinated with the electroeluted 66-64 kda antigen or treated with polyclonal and monoclonal antibodies, respectively. the identification and c ... | 1994 | 11178405 |
| [potency testing of erysipelas vaccines in mice. elisa vs. challenge] | vaccines are widely used for the prophylaxis of swine erysipelas. according to national pharmacopoiea requirements, potency of these biologicals has to be tested in a mouse challenge model. many animals are needed and the test causes much suffering. the development of an enzyme linked immunosorbet assay (elisa) using an alkaline extract of erysipelothrix rhusiopathiae as antigen for coating the microtitre plate allows us to measure antibody titres in the serum of vaccinated mice. by comparing th ... | 1994 | 11178406 |
| a serological survey on erysipelas in chickens by growth agglutination test. | from march to april 1992, 200 culled chickens sent to an abattoir in tokyo, japan were examined for the presence of growth agglutinating antibody in the serum. in the serological survey, 11 (5.5%) of 200 chicken sera had high growth agglutination titres against erysipelothrix rhusiopathiae, ranging from 1:16 to 1:128. these findings indicate a high rate of erysipelas infection among chickens in the field. | 2000 | 11204134 |
| computerized comparison of the protein compositions of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum strains. | protein profiles of six erysipelothrix rhusiopathiae strains, five erysipelothrix tonsillarum strains and three erysipelothrix strains of uncertain taxonomic position were studied by sodium dodecyl sulphate-polyactylamide gel electrophoresis (sds-page). in a computerized comparison of the protein patterns of the strains, the level of similarity between the strains was determined. the sds-page protein bands were divided into 14 groups based on molecular weight. the relative distribution of protei ... | 2001 | 11254101 |
| antimicrobial susceptibilities of erysipelothrix rhusiopathiae isolated from pigs with swine erysipelas in japan, 1988-1998. | the susceptibility to 21 antimicrobial agents of 214 strains of erysipelothrix rhusiopathiae isolated from pigs affected with swine erysipelas in japan between 1988 and 1998 was determined. ampicillin, cloxacillin, benzylpenicillin, ceftiofur, tylosin, enrofloxacin and danofloxacin were the most active agents [minimum inhibitory concentrations (mics); < or = 0.025-0.78 microgram/ml], followed by cefazolin, virginiamycin, tiamulin, chloramphenicol, florphenicol and oxolinic acid (mics; 0.1-25 mic ... | 2001 | 11315521 |
| distribution of antibody against erysipelothrix rhusiopathiae in cattle. | serum samples collected from 854 cattle in nine prefectures of japan, from hokkaido to okinawa, between 1988 and 1992 were examined for presence of antibodies against erysipelothrix rhusiopathiae by growth agglutination test. most of the sera showed positive reactions, and the antibody titers ranged from below 4 to above 128. seventy-six percent of the sera showed titers of 32 or above, and 34% showed titers of 128 or above. the titers had a tendency to be higher in the south and lower in the no ... | 2001 | 11329469 |
| induction of cross-protection in mice against dolphin erysipelothrix rhusiopathiae isolates with a swine commercial vaccine. | erysipelothrix rhusiopathiae is well known to cause disease in dolphins. this disease occurs either in an peracute way, leading to mortality even before clinical signs are observed or in a sub-acute way, characterized by rhomboidal skin lesions, that can be treated with penicillin or its derivatives. commercial swine vaccines, containing inactivated serotype 2 strains, are currently used for vaccination but it is not known whether these vaccines induce protection against e. rhusiopathiae isolate ... | 2001 | 11337140 |
| [mitral valve endocarditis caused by erysipelothrix rhusiopathiae]. | a 67 year-old country woman was admitted to the hospital because of a four weeks history of continuous catarrh, arthralgia and fever. recently, she had also developed upper abdominal pain after oral ibuprofen treatment. the clinical examination showed a patient of impaired general condition. the heart and lungs were auscultatory normal and there were no signs of dyspnea, cyanosis or inflammatory skin lesions. | 2001 | 11347005 |
| structural investigations on the cell surface of erysipelothrix rhusiopathiae. | the cell wall of erysipelothrix rhusiopathiae was investigated. the corrected structure of the murein, which was published earlier, is reported here. the murein belongs to the b1delta type. it has l-alanine in position three of the peptide subunit and is crosslinked via a glycine-l-lysine-l-lysine interpeptide bridge. as expected by the complex serological situation in e. rhusiopathiae the carbohydrate moiety of the cell wall proofed to be very intricate. as accessory polymers polysaccharide(s) ... | 2001 | 11403395 |
| serovars of erysipelothrix species isolated from the tonsils of healthy cattle in japan. | serovars of 79 erysipelothrix isolates recovered from the tonsils of healthy slaughtered cattle over a 1-year period in japan were determined by an agar double-diffusion precipitation system using typing sera representing all the known serovars, 1 through 23 and type n, of erysipelothrix. a total of 43 out of the 79 erysipelothrix isolates could be classified into nine serovars but the remaining 36 isolates were untypable. of 42 isolates identified as erysipelothrix rhusiopathiae, 4, 6, 2, 3, 1, ... | 2001 | 11423200 |
| aortic valve endocarditis with paravalvular abscesses caused by erysipelothrix rhusiopathiae. | | 2001 | 11681442 |
| [endocarditis caused by erysipelothrix rhusiopathiae: a rare professional disease difficult to diagnose]. | | 2001 | 11709133 |
| erysipelothrix rhusiopathiae ys-1 as a live vaccine vehicle for heterologous protein expression and intranasal immunization of pigs. | we have developed a system in which a foreign antigen is delivered and expressed on the surface of an attenuated strain of erysipelothrix rhusiopathiae ys-1 and have examined the ability of a such recombinant e. rhusiopathiae strain to function as a mucosal vaccine vector. the c-terminal portion, including two repeat regions, r1 and r2, of the p97 adhesin of mycoplasma hyopneumoniae strain e-1 was successfully translocated and expressed on the e. rhusiopathiae ys-1 cell surface after it was fuse ... | 2002 | 11748187 |
| tiamulin activity against fastidious and nonfastidious veterinary and human bacterial isolates: initial development of in vitro susceptibility test methods. | tiamulin is a pleuromutilin derivative used in veterinary practice for the control and specific therapy of infections in swine. this report summarizes studies to establish standardized susceptibility testing methods, interpretive criteria, and reagent details for use in veterinary methods recently developed by the national committee for clinical laboratory standards (nccls) (standards m31-a and m37-a, nccls, wayne, pa., 1999). a total of 636 fastidious and nonfastidious animal and human pathogen ... | 2002 | 11825957 |
| [erysipelothrix rhusiopathiae bacteremia without endocarditis or evidence of the entry point in an immunocompet woman]. | | 2002 | 11904094 |
| hyaluronidase is not essential for the lethality of erysipelothrix rhusiopathiae infection in mice. | to investigate the role of hyaluronidase in the pathogenicity of erysipelothrix rhusiopathiae, transposon tn916 was transferred from enterococcus faecalis cg110 to a virulent strain of e. rhusiopathiae, and hyaluronidase-deficient mutants were isolated. a virulence assay in the mice showed that of the seven hyaluronidase-deficient mutants tested, six mutants were avirulent, but that one mutant, designated ast121, was as virulent as its parental strain. western immunoblotting with a monoclonal an ... | 2002 | 11913558 |
| the detection and recovery of erysipelothrix spp. in meat and abattoir samples in western australia. | to investigate the occurrence of erysipelothrix rhusiopathiae and other erysipelothrix spp. in abattoir and meat samples in western australia. | 2002 | 11972687 |
| case study: chronic erysipelas of the sow--a subclinical manifestation of reproductive problems. | systemic infections with erysipelothrix rhusiopathiae are usually associated with skin lesions, vegetative endocarditis and arthritis, but they can also cause reproductive symptoms such as abortion, increased stillbirths, and smaller litter size. in a large hungarian breeding unit that had ceased to vaccinate the sows against erysipelas, an increased incidence of pre- and post-partal vulval discharge, increased weaning-to-oestrus intervals, decreased farrowing rates, and reductions in total numb ... | 2002 | 11975752 |
| mucosal immunoadjuvant activity of the low toxic recombinant escherichia coli heat-labile enterotoxin produced by bacillus brevis for the bacterial subunit or component vaccine in pigs and cattle. | a gene encoding the mature escherichia coli heat-labile enterotoxin (lt) lacking the nick site in the a subunit by deleting tripeptides was introduced in a vector pnh301 and expressed extracellularly as mutant molecule of holotoxin at high levels in bacilus brevis hpd31-s5 of the host bacterium. the mucosal adjuvant activities of the produced mutant lt (mlt) preparation were studied in pigs and cattle. intranasal immunization of pigs with the recombinant subunit vaccine of erysipelothrix rhusiop ... | 2002 | 12072249 |
| [infective endocarditis with perivalvular abscess in a patient with erysipelothrix rhusiopathiae bacteremia]. | the bacterial species erysipelothrix rhusiopathiae is found worldwide as a commensal or a pathogen in a variety of animals. one well-defined pattern of human infection is an uncommon bacteremic form, with or without cutaneous involvement, usually complicated by endocarditis. we report the case of a 38-year-old male butcher with e. rhusiopathiae bacteremia, native aortic valve endocarditis and perivalvular abscess. the patient was released after six weeks of intravenous ceftriaxone and aortic val ... | 2002 | 12150010 |
| [do arthralgia and arthritis manifest during or after an infection with erysipelothrix rhusiopathiae (erysipelas)]. | | 2002 | 12219640 |